Spg021283 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021283
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	scaffold6: 47597417 .. 47599146 (-)
RNA-Seq Expression	Spg021283
Synteny	Spg021283

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTATTAAAAAAAAAAAAAAAAAATCTCTTTAATTTTTCAATTTCATATTCCCCAAACCAAAATCAAAACCAACCTCAAAGTTTCAGCCTCTTCCATTTCCATGCTTGAAATCTTCAAGTACCTCGTCGGTTCCGCCGGAGCAAGCGGCTACGGTTCCAAATCCACCGCCGACGAGGTCACTCAAGCATGTTCTCATCTGCACTCCGTCACCGCCATAATCACAGGTGATTTTCTTTGAATTCAGAATTCAGAATTTCGATTGCATCATATTAAACTGAATTCTGTCGCTAACAAATTTTGGCGCAGGAGCGACGTCCGGAATCGGAGAGGAGACGGCGAGAGTATTAGCCAAGCGTGGCGCCAGGATCGTCATTCCGGCTCGCGACCTCAAAGCCGCGGAGGAAGCGAAGGCGCGAATCGTGTCGGAATCGGAATGTATCGATCATTCACGAATCACGGTGATGGCTCTCGATCTTAGTTCTCTGAATTCCGTCACGAACTTCGTCTCCGAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATGTAATTCATCCAAAACTTCGCTAATTCACTCCGTTGAATCGAACGAACTAACGACATTAATTGATTTTGAACAAAACTAAAAGCCGATTTCGTTATTTTTATCAGAAACAATGCCGGTAGGTTCTGTTACGAACATGCGATTTCTGAAGACGGAATTGAGATGACGTTCGCTACTAATTATCTAGGTAAGTACGTAGAACAAAATTTATGTTCATCTCTAATTCATGAAGCCTTTTACCATTATTGATCGAATCGAGCATGCAGGTCATTTCCTGTTGACGAAACTTTTGCTGAATAAGATGATCGAAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTCTCCGGCGACATATTTGAATATCTCGACCAGATAAGCTCGAACAACAGGTGTGATTAATTTTCCTTTGCTTACGTATTAATGGCGGTCTGATAGCGATACCGCTTTTCACTCGCAGCAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCCAATGTCTTGCACGCCATCCAACTCGCTCACAGACTCCAGGTTCTTCCATATAGATATCAATTGGTATTAATTTTCATAGAATGATTTCACGTACGAGTTGGTTTTTGACAAAATCAAAACAGGAAATGGAGGCAAATGTTACGGTAAACTGTGTTCATCCGGGCGTAGTGAAGACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGGTATTGATCAAAGCTCCTGCATGGAAATTAATCAATCTAAAACGTATTAAAGTCTATATAATTAATTAATTGATGAGAAAATACAGATTTGATTTTCTACATGGCGTCCAAATCAAAGTTGTTGAAGACAATTCCTCAAGGAGCAGCAACAAGTTGTTACGTAGCCGCAAATCGGAAAGTGGAAAATGTGAATGGAAAATACTTTGCGGATTGCAACGAACAAGAAAGCTGCGAATCCAAATTAGGTACCAAATCCACTCACTTGGCTGCACGCCTATGGTCCGCCTCTGAAACCATCGTCTCCACTCGTCCAGGTAACCCTGAAGAACTCTAATCTCAACAATTTGATCAACTCAATTCAACGAATCAATTGGATTGAGTTTGTTTTATGAATTTTCATATTGATTTGATTTGAGTTCAAATAAATAATAAACACAT

mRNA sequence

ATGCTTGAAATCTTCAAGTACCTCGTCGGTTCCGCCGGAGCAAGCGGCTACGGTTCCAAATCCACCGCCGACGAGGTCACTCAAGCATGTTCTCATCTGCACTCCGTCACCGCCATAATCACAGGAGCGACGTCCGGAATCGGAGAGGAGACGGCGAGAGTATTAGCCAAGCGTGGCGCCAGGATCGTCATTCCGGCTCGCGACCTCAAAGCCGCGGAGGAAGCGAAGGCGCGAATCGTGTCGGAATCGGAATGTATCGATCATTCACGAATCACGGTGATGGCTCTCGATCTTAGTTCTCTGAATTCCGTCACGAACTTCGTCTCCGAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATAAACAATGCCGGTAGGTTCTGTTACGAACATGCGATTTCTGAAGACGGAATTGAGATGACGTTCGCTACTAATTATCTAGGTCATTTCCTGTTGACGAAACTTTTGCTGAATAAGATGATCGAAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTCTCCGGCGACATATTTGAATATCTCGACCAGATAAGCTCGAACAACAGCAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCCAATGTCTTGCACGCCATCCAACTCGCTCACAGACTCCAGGAAATGGAGGCAAATGTTACGGTAAACTGTGTTCATCCGGGCGTAGTGAAGACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAAGTTGTTGAAGACAATTCCTCAAGGAGCAGCAACAAGTTGTTACGTAGCCGCAAATCGGAAAGTGGAAAATGTGAATGGAAAATACTTTGCGGATTGCAACGAACAAGAAAGCTGCGAATCCAAATTAGGTACCAAATCCACTCACTTGGCTGCACGCCTATGGTCCGCCTCTGAAACCATCGTCTCCACTCGTCCAGGTAACCCTGAAGAACTCTAA

Coding sequence (CDS)

ATGCTTGAAATCTTCAAGTACCTCGTCGGTTCCGCCGGAGCAAGCGGCTACGGTTCCAAATCCACCGCCGACGAGGTCACTCAAGCATGTTCTCATCTGCACTCCGTCACCGCCATAATCACAGGAGCGACGTCCGGAATCGGAGAGGAGACGGCGAGAGTATTAGCCAAGCGTGGCGCCAGGATCGTCATTCCGGCTCGCGACCTCAAAGCCGCGGAGGAAGCGAAGGCGCGAATCGTGTCGGAATCGGAATGTATCGATCATTCACGAATCACGGTGATGGCTCTCGATCTTAGTTCTCTGAATTCCGTCACGAACTTCGTCTCCGAATTCGAGTCCTTACATTTGCCTCTTAATCTCCTCATAAACAATGCCGGTAGGTTCTGTTACGAACATGCGATTTCTGAAGACGGAATTGAGATGACGTTCGCTACTAATTATCTAGGTCATTTCCTGTTGACGAAACTTTTGCTGAATAAGATGATCGAAACGGCCAAATCGACGGGAATCCAAGGCCGAATAGTGAATGTGACGTCCAACATCCACAGCTGGTTCTCCGGCGACATATTTGAATATCTCGACCAGATAAGCTCGAACAACAGCAGAGAGTACGACGCGACACGTGCTTACGCGTTCTCCAAGCTCGCCAATGTCTTGCACGCCATCCAACTCGCTCACAGACTCCAGGAAATGGAGGCAAATGTTACGGTAAACTGTGTTCATCCGGGCGTAGTGAAGACCAAATTGAACAGAGATCGTGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAAGTTGTTGAAGACAATTCCTCAAGGAGCAGCAACAAGTTGTTACGTAGCCGCAAATCGGAAAGTGGAAAATGTGAATGGAAAATACTTTGCGGATTGCAACGAACAAGAAAGCTGCGAATCCAAATTAGGTACCAAATCCACTCACTTGGCTGCACGCCTATGGTCCGCCTCTGAAACCATCGTCTCCACTCGTCCAGGTAACCCTGAAGAACTCTAA

Protein sequence

MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFADCNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPEEL

Homology

[Close]

BLAST of Spg021283 vs. NCBI nr
Match: XP_011650304.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >KAE8650011.1 hypothetical protein Csa_011335 [Cucumis sativus])

HSP 1 Score: 566.2 bits (1458), Expect = 1.9e-157
Identity = 292/340 (85.88%), Postives = 312/340 (91.76%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGSKSTA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAK GA
Sbjct: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKGGA 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAAEEAKARIVSE EC DHSRITVM LDLSSLNSV NFVSEFESL+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDHSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETA+STGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAESTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNS--REYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVN 240
           IHSWFSGD+FEYL QIS NN+  REYDATRAYAFSKLAN+LH +QLAHRLQEM+ANVTVN
Sbjct: 181 IHSWFSGDVFEYLSQISRNNNKYREYDATRAYAFSKLANLLHTVQLAHRLQEMKANVTVN 240

Query: 241 CVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYF 300
           CVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA +RKVENVNGKYF
Sbjct: 241 CVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKYF 300

Query: 301 ADCNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           ADCNEQ SCES   TKS HLA RLWS SE IVS   G P+
Sbjct: 301 ADCNEQGSCES--NTKSAHLAERLWSTSEIIVSNLTGKPQ 338

[Close]

BLAST of Spg021283 vs. NCBI nr
Match: KAA0052978.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 564.3 bits (1453), Expect = 7.1e-157
Identity = 291/342 (85.09%), Postives = 313/342 (91.52%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAAEEAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS----SNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVT 240
           IHSWFSGDIFEYL QIS    +NNSREYDATRAY+FSKLAN+LH +QLAHRLQEM+ANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGK 300
           VNCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA +RKVENVNGK
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFADCNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           YF DCNEQ SCES   TKS HLA RLWSASE I+S   GNP+
Sbjct: 301 YFEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 340

[Close]

BLAST of Spg021283 vs. NCBI nr
Match: XP_022923250.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6577763.1 Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 563.9 bits (1452), Expect = 9.3e-157
Identity = 287/338 (84.91%), Postives = 312/338 (92.31%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAAEEAKARIVSES+C DHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLH +QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFAD 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLKTIP+GAATSCYVA NR VENVNG YFAD
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVST  G P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Spg021283 vs. NCBI nr
Match: XP_008449162.1 (PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 290/341 (85.04%), Postives = 313/341 (91.79%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAAEEAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS---SNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTV 240
           IHSWFSGDIFEYL QIS   +NN+REYDATRAY+FSKLAN+LH +QLAHRLQEM+ANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKY 300
           NCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA +RKVENVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           F DCNEQ SCES   TKS HLA RLWSASE I+S   GNP+
Sbjct: 301 FEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 339

[Close]

BLAST of Spg021283 vs. NCBI nr
Match: XP_023552711.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 561.2 bits (1445), Expect = 6.0e-156
Identity = 285/338 (84.32%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAAEEAKARIVSES+C DHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLH +QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFAD 300
           HPGVVKT LNR+REGFVTDLIFYM SKS+LLKTIP+GAAT+CYVA NR VENVNG YFAD
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVST  G P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Spg021283 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.8e-68
Identity = 153/335 (45.67%), Postives = 214/335 (63.88%), Query Frame = 0


Query: 2   LEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGAR 61
           +EI+  + G AG SGYGS STA++VT +    H +TAIITG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKH-LTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLL 121
           ++I +R+ KAA ++K  I+   +   ++RI  + LDLSS+ SV +F+ +F +L++PLN+L
Sbjct: 61  VIIASRNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 181
           INNAG       +SEDGIE  FATN++GHFLLT LLL+KM  +A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNC 241
           H  ++  G +F+Y+     N+   Y   +AY  SKLAN+LH+  L+ +LQE   N+T+N 
Sbjct: 181 HTYTYTEGIMFDYI-----NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINS 240

Query: 242 VHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFA 301
           VHPG++ T L R        +    A    L K IPQGAAT+CYVA +  +++V GKYFA
Sbjct: 241 VHPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFA 300

Query: 302 DCNEQESCESKLGTKSTHLAARLWSASETIVSTRP 335
           DCN        +    T LA +LW  S  +V + P
Sbjct: 301 DCNVTTPSNFAI---DTTLADKLWDFSIKLVESLP 320

[Close]

BLAST of Spg021283 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.4e-68
Identity = 153/335 (45.67%), Postives = 212/335 (63.28%), Query Frame = 0


Query: 2   LEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGAR 61
           +EI+  + G AG SGYGS STA++VT +    H +TAIITG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKH-LTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLL 121
           ++I AR+ KAA ++K  I+   +   ++RI  + LDLSS+ SV +F+ +F +L++PLN+L
Sbjct: 61  VIIAARNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 181
           INNAG       +SEDGIE  FATN++GHFLLT LLL+KM  +A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNC 241
           H  ++  G +F+Y+     N+   Y   +AY  SKLAN+LH+  L+ +LQE   N+T+N 
Sbjct: 181 HTYTYTEGIMFDYI-----NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINS 240

Query: 242 VHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFA 301
           VHPG++ T L R        +    A    L K IPQGAAT+CYVA +  ++ V GKYF 
Sbjct: 241 VHPGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFT 300

Query: 302 DCNEQESCESKLGTKSTHLAARLWSASETIVSTRP 335
           DCN        +    T LA +LW  S  +V + P
Sbjct: 301 DCNVTTPSNFAI---DTTLADKLWDFSIKLVDSLP 320

[Close]

BLAST of Spg021283 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.9e-62
Identity = 145/329 (44.07%), Postives = 203/329 (61.70%), Query Frame = 0


Query: 7   YLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIVIPA 66
           +  GS GASG+ S+STA+EVT        +TAI+TGA+SGIG ETARVL+ RG  +V+  
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTHGVDGT-GLTAIVTGASSGIGVETARVLSLRGVHVVMAV 61

Query: 67  RDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAG 126
           R+  +  + K  IV +   +  +++ VM LDLSS+ SV  F SE++S  LPLNLLINNAG
Sbjct: 62  RNTDSGAKVKEDIVKQ---VPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAG 121

Query: 127 RFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIHSWFS 186
                  +S+D IE+ FATN+LGHFLLTKLLL+ M  T++ +  +GRIVN++S  H  FS
Sbjct: 122 IMACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHR-FS 181

Query: 187 GDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCVHPGVVK 246
                  D+I  N+   Y + RAY  SKL NVLHA +L  +L+E   N+T N +HPG + 
Sbjct: 182 YPEGVRFDKI--NDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIM 241

Query: 247 TKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFADCNEQES 306
           T L R    ++   +  +A    +LK++PQGAAT+CYVA N +V  V+G+YF D N  + 
Sbjct: 242 TNLGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKP 301

Query: 307 CESKLGTKSTHLAARLWSASETIVSTRPG 336
                  K T LA ++W  S  +  ++ G
Sbjct: 302 LPL---VKDTELAKKVWDFSTKLTDSQSG 318

[Close]

BLAST of Spg021283 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 5.1e-57
Identity = 139/321 (43.30%), Postives = 195/321 (60.75%), Query Frame = 0


Query: 13  GASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIVIPARDLKAA 72
           G SG+   STA++VT        +TAI+TGA+SGIG ET RVLA RGA +++  R++ AA
Sbjct: 9   GVSGFSGSSTAEQVTHGID-ATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  EEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAGRFCYEH 132
           ++ K  I+ +   I  +++  + LDLSSL+SV  F SEF S   PLN+LINNAG      
Sbjct: 69  KDVKDTILKD---IPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPF 128

Query: 133 AISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIHSWFSGDIFEY 192
            +S+D IE+ FATN++GHFLLT LLL+ M +T + +  +GRIVNV S  H +   +   +
Sbjct: 129 KLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRF 188

Query: 193 LDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCVHPGVVKTKLNRD 252
            D+I  N+   Y+  RAY  SKLANVLHA QL   L+E   N+T N +HPG + T L R 
Sbjct: 189 -DKI--NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRH 248

Query: 253 REGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFADCNEQESCESKLG 312
               V  LI  +     +LK + QGAAT+CYVA + +V+ V+G+YF+D N  ++      
Sbjct: 249 NSA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPH--- 308

Query: 313 TKSTHLAARLWSASETIVSTR 334
            K   LA +LW  S  +V  +
Sbjct: 309 GKDVDLAKKLWDFSINLVKQK 316

[Close]

BLAST of Spg021283 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.9e-41
Identity = 122/310 (39.35%), Postives = 173/310 (55.81%), Query Frame = 0


Query: 33  LHSVTAIITGATSGIGEETARVLAKRGARIVIPARDLKAAEEAKARIVSE----SECIDH 92
           +H  T +ITGA SG+G  TA  L + GAR+++  RD   AEEA  ++  E    +EC   
Sbjct: 41  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPE 100

Query: 93  ------SRITVMALDLSSLNSVTNFVSEFESLHLPLNLLINNAGRFCYEHAISEDGIEMT 152
                   + V  LDL+SL SV  F  E       L++LINNAG F   +  +EDG EM 
Sbjct: 101 PGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQ 160

Query: 153 FATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNIHSWFSGDI-FEYLDQISSNNS 212
           F  N+LGHFLLT LLL  +  +A S     RIV V+S ++ +  GDI F+ L     N+ 
Sbjct: 161 FGVNHLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GDINFDDL-----NSE 220

Query: 213 REYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCVHPGVVKTKLNRD-REGFVTDL 272
           + Y+ +  Y+ SKLAN+L   +LA RL+    NVTVN +HPG+V+T L R      +   
Sbjct: 221 QSYNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKP 280

Query: 273 IFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFADCNEQESCESKLGTKSTHLAA 331
           +F + S +   KT  +GA TS Y+A++ +VE V+G+YF DC E+E     +      +A 
Sbjct: 281 LFNLVSWA-FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAM---DESVAR 332

[Close]

BLAST of Spg021283 vs. ExPASy TrEMBL
Match: A0A5A7UCR1 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00880 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.4e-157
Identity = 291/342 (85.09%), Postives = 313/342 (91.52%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAAEEAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS----SNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVT 240
           IHSWFSGDIFEYL QIS    +NNSREYDATRAY+FSKLAN+LH +QLAHRLQEM+ANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGK 300
           VNCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA +RKVENVNGK
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFADCNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           YF DCNEQ SCES   TKS HLA RLWSASE I+S   GNP+
Sbjct: 301 YFEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 340

[Close]

BLAST of Spg021283 vs. ExPASy TrEMBL
Match: A0A6J1EB90 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.5e-157
Identity = 287/338 (84.91%), Postives = 312/338 (92.31%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAAEEAKARIVSES+C DHSRITVMALDLSS+NSV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLH +QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFAD 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLKTIP+GAATSCYVA NR VENVNG YFAD
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVST  G P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Spg021283 vs. ExPASy TrEMBL
Match: A0A1S3BLF4 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491110 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.9e-157
Identity = 290/341 (85.04%), Postives = 313/341 (91.79%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+ KYLVGSAGASGYGS STA+EVTQ+ +HL+S+TAI+TGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RIVIPAR+LKAAEEAKARIVSE EC D+SRITVM LDLSSLNSV NFVSEFE+L+LPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQIS---SNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTV 240
           IHSWFSGDIFEYL QIS   +NN+REYDATRAY+FSKLAN+LH +QLAHRLQEM+ANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKY 300
           NCVHPGVV+T LNRDREGF+ DLIF+MASKSKLLKTIPQ AATSCYVA +RKVENVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           F DCNEQ SCES   TKS HLA RLWSASE I+S   GNP+
Sbjct: 301 FEDCNEQGSCES--NTKSAHLAERLWSASEIIISNLTGNPQ 339

[Close]

BLAST of Spg021283 vs. ExPASy TrEMBL
Match: A0A6J1CWG8 (short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014858 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 8.5e-156
Identity = 296/340 (87.06%), Postives = 307/340 (90.29%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLEI KYLVGSAGASGYGSKSTA EVTQ+C HL SVTAIITGATSGIGEETARVLAKRGA
Sbjct: 1   MLEILKYLVGSAGASGYGSKSTAVEVTQSCPHLCSVTAIITGATSGIGEETARVLAKRGA 60

Query: 61  RIVIPARDLKAAEEAKARI--VSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPL 120
           RIV+PAR+LKAAEEAKARI   SESEC DHSRITVM LDLSSLNSV NFVS+FESL+LPL
Sbjct: 61  RIVLPARNLKAAEEAKARIKSESESECSDHSRITVMELDLSSLNSVRNFVSKFESLNLPL 120

Query: 121 NLLINNAGRFCYEHAISEDGIEMTFATNYL-----------GHFLLTKLLLNKMIETAKS 180
           NLLINNAGRFCYEHAISEDGIEMTF TNYL           GHFLLTKLLLNKMIETAK 
Sbjct: 121 NLLINNAGRFCYEHAISEDGIEMTFTTNYLGQYIGEQILFSGHFLLTKLLLNKMIETAKL 180

Query: 181 TGIQGRIVNVTSNIHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHR 240
           TGIQGRIVN+TSNIHSWFSGDIFEYL +IS +NSREYDATRAYA SKLANVLH +QLAHR
Sbjct: 181 TGIQGRIVNLTSNIHSWFSGDIFEYLGRISRSNSREYDATRAYALSKLANVLHTVQLAHR 240

Query: 241 LQEMEANVTVNCVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAAN 300
           LQE EANVTVNCVHPGVV+TKLNRDREGFVTDLIFYMASK+KLLKTIPQGAATSCYVA N
Sbjct: 241 LQETEANVTVNCVHPGVVRTKLNRDREGFVTDLIFYMASKTKLLKTIPQGAATSCYVATN 300

Query: 301 RKVENVNGKYFADCNEQESCESKLGTKSTHLAARLWSASE 328
           R VENVNGKYFADCNEQ SCESKLGTKS  LAARLWSASE
Sbjct: 301 RNVENVNGKYFADCNEQGSCESKLGTKSAPLAARLWSASE 340

[Close]

BLAST of Spg021283 vs. ExPASy TrEMBL
Match: A0A6J1HN85 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.5e-155
Identity = 285/338 (84.32%), Postives = 310/338 (91.72%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           MLE+FKYLVGSAGASGYGSKSTA+EV+++C  ++S+TAI+TGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           RI+IPAR+LKAAEEAKARIVSES C D SRITVMALDLSS+ SV NFVS FESL+LPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAGRFCYEHA+SEDGIEMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCV 240
           IHSWFSGDIFEYL QIS  NSR YD+TRAYAFSKLANVLH +QLAH+LQEM+ANVTVNCV
Sbjct: 181 IHSWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFAD 300
           HPGVVKT LNR+REGFVTDLIFYMASKS+LLK IP+GAATSCYVA NR VENVNGKYFAD
Sbjct: 241 HPGVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFAD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVSTRPGNPE 339
           CN+Q SCE K  TKS HLA  LWSASETIVST  G P+
Sbjct: 301 CNDQGSCECK--TKSAHLAEGLWSASETIVSTLTGKPQ 334

[Close]

BLAST of Spg021283 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 424.5 bits (1090), Expect = 8.2e-119
Identity = 226/331 (68.28%), Postives = 272/331 (82.18%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           M+E  KYL+G+AGASG+GSKSTA+EVT+ C  L S+TA+ITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCD-LRSITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           R++ PAR++KAAEEAK RIVSE      + I VM LDLSS+ SV NFV++FESL LPLNL
Sbjct: 61  RLIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAG+  +EHAISEDGIEMTFATNYLGHFLLT LLLNKMI+TA+ TG+QGRIVNVTS 
Sbjct: 121 LINNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSG 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCV 240
           IH WFSGD+ EYL  IS     ++DATRAYA SKLANVLH  +L+ RLQ++ ANVTVNCV
Sbjct: 181 IHGWFSGDLIEYLRLISQPKC-QFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFAD 300
           HPGVV+T+L RDREG +TDL+F++A  SKL+KT+PQ AAT+CYVA N ++ NV+GKYF D
Sbjct: 241 HPGVVRTRLTRDREGLLTDLVFFLA--SKLVKTVPQAAATTCYVATNPRLVNVSGKYFTD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVS 332
           CN  E+  S LGT S+  A +LW+ASE +V+
Sbjct: 301 CN--ETTPSGLGTNSSE-ATKLWAASEILVT 321

[Close]

BLAST of Spg021283 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 412.9 bits (1060), Expect = 2.5e-115
Identity = 220/331 (66.47%), Postives = 266/331 (80.36%), Query Frame = 0


Query: 1   MLEIFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGA 60
           M+E  K+L+GS G SG+GS+STAD VT   S L S+TAIITGATSGIG ETARVLAKRGA
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVT-CNSDLRSLTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNL 120
           R+V+PAR +K AEE KARI+SE      + I VM LDLSSL SV  FV +FESL+LPLN+
Sbjct: 61  RLVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNI 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180
           LINNAG++ ++HA+SEDG+EMTFATNYLGHFLLTKLLL KMIETA  TG+QGRIVNVTS 
Sbjct: 121 LINNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSV 180

Query: 181 IHSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCV 240
           +HSWFSGD+ +YL  IS NN R YDATRAYA SKLANVLH ++L+  L +M+ANVT NCV
Sbjct: 181 VHSWFSGDMLQYLADISRNN-RNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCV 240

Query: 241 HPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFAD 300
           HPG+VKT+L RDREG VTDL+F++   SKLLK++PQ AAT+CYVA + ++ NV GKYF+D
Sbjct: 241 HPGIVKTRLTRDREGVVTDLVFFLT--SKLLKSVPQAAATTCYVATSPRLRNVCGKYFSD 300

Query: 301 CNEQESCESKLGTKSTHLAARLWSASETIVS 332
           CNE  S  SK G+ +   A RLW+AS+ +VS
Sbjct: 301 CNEARS--SKSGSCNLK-AQRLWTASDLLVS 321

[Close]

BLAST of Spg021283 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.2e-80
Identity = 170/332 (51.20%), Postives = 228/332 (68.67%), Query Frame = 0


Query: 6   KYLVGSAGASGYGSKSTADEVTQ----ACSHLHSVTAIITGATSGIGEETARVLAKRGAR 65
           +YL G AG +G+GS+STA++VTQ     CSHL   TAIITG TSGIG ETARVLAKRG R
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHL---TAIITGGTSGIGAETARVLAKRGVR 65

Query: 66  IVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLL 125
           +V+  RD+K AE  K RI+ E+   D   I +  +DLSSL+SV  F S+F S  LPLN+L
Sbjct: 66  VVMAVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNIL 125

Query: 126 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 185
           INNAG F      SE+ IE+TFATN+LGH+LLT++L+ KMI+TA+ +GI+GRI+N++S I
Sbjct: 126 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 185

Query: 186 HSWFSGDIFEY---LDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVN 245
           H+W   D F +   L  IS      Y+ TRAYA SKLA +LHA  L+ +L++  ANVT+N
Sbjct: 186 HNWVKPDCFSFPKLLHPIS-----RYNGTRAYAQSKLATILHAKALSKQLKDRNANVTIN 245

Query: 246 CVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYF 305
            VHPG+VKT + R  +G  TD +F +A  SKLLK+I QGAAT+CYVA + + + ++GKYF
Sbjct: 246 AVHPGIVKTGIIRAHKGLFTDSLFLIA--SKLLKSISQGAATTCYVALSNETKGLSGKYF 305

Query: 306 ADCNEQESCESKLGTKSTHLAARLWSASETIV 331
           ADCNE    +        ++A +L + S  ++
Sbjct: 306 ADCNETNCSDL---ANDEYVALKLCTQSRALI 321

[Close]

BLAST of Spg021283 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 4.7e-74
Identity = 163/331 (49.24%), Postives = 222/331 (67.07%), Query Frame = 0


Query: 4   IFKYLVGSAGASGYGSKSTADEVTQACSHLHSVTAIITGATSGIGEETARVLAKRGARIV 63
           ++  + G  G SG+GS STA+EVTQ      ++TAIITG T GIG ETARVL+KRGA +V
Sbjct: 3   LYSLITGRRGPSGFGSASTAEEVTQGID-ATNLTAIITGGTGGIGMETARVLSKRGAHVV 62

Query: 64  IPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLLIN 123
           I AR++ AAE AK  I+ ++    ++R+T++ LDLSS+ S+  FV EF +LHLPLNLLIN
Sbjct: 63  IGARNMGAAENAKTEILRQNA---NARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLIN 122

Query: 124 NAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTS--NI 183
           NAG     + +SEDGIE+ FATN++GHFLLT LLL+ M  TAK++G++GRI+NV+S  +I
Sbjct: 123 NAGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHI 182

Query: 184 HSWFSGDIFEYLDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVNCVH 243
           +++  G  F+ ++ I S     Y   RAY  SKLAN+LHA +L+ +LQE   N+T N VH
Sbjct: 183 YTYQEGIQFDSINDICS-----YSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVH 242

Query: 244 PGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQGAATSCYVAANRKVENVNGKYFADC 303
           PG++ T L +     +  L F+      L K IPQGAAT+CYVA +  V+ V GKYFADC
Sbjct: 243 PGLILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADC 302

Query: 304 NEQESCESKLGTKSTHLAARLWSASETIVST 333
           N  E   SKL    T LA +LW  S  ++++
Sbjct: 303 N--EVTPSKLARDET-LAQKLWDFSVKLINS 318

[Close]

BLAST of Spg021283 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.5e-69
Identity = 150/278 (53.96%), Postives = 195/278 (70.14%), Query Frame = 0


Query: 6   KYLVGSAGASGYGSKSTADEVTQ----ACSHLHSVTAIITGATSGIGEETARVLAKRGAR 65
           +YL G AG +G+GS+STA++VTQ     CSHL   TAIITG TSGIG ETARVLAKRG R
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHL---TAIITGGTSGIGAETARVLAKRGVR 65

Query: 66  IVIPARDLKAAEEAKARIVSESECIDHSRITVMALDLSSLNSVTNFVSEFESLHLPLNLL 125
           +V+  RD+K AE  K RI+ E+   D   I +  +DLSSL+SV  F S+F S  LPLN+L
Sbjct: 66  VVMAVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNIL 125

Query: 126 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSNI 185
           INNAG F      SE+ IE+TFATN+LGH+LLT++L+ KMI+TA+ +GI+GRI+N++S I
Sbjct: 126 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 185

Query: 186 HSWFSGDIFEY---LDQISSNNSREYDATRAYAFSKLANVLHAIQLAHRLQEMEANVTVN 245
           H+W   D F +   L  IS      Y+ TRAYA SKLA +LHA  L+ +L++  ANVT+N
Sbjct: 186 HNWVKPDCFSFPKLLHPIS-----RYNGTRAYAQSKLATILHAKALSKQLKDRNANVTIN 245

Query: 246 CVHPGVVKTKLNRDREGFVTDLIFYMASKSKLLKTIPQ 277
            VHPG+VKT + R  +G  TD +F +A  SKLLK+I Q
Sbjct: 246 AVHPGIVKTGIIRAHKGLFTDSLFLIA--SKLLKSISQ 270

The following BLAST results are available for this feature:

BLAST of Spg021283 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650304.1	1.9e-157	85.88	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >...	[more]
KAA0052978.1	7.1e-157	85.09	short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa]	[more]
XP_022923250.1	9.3e-157	84.91	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch...	[more]
XP_008449162.1	1.2e-156	85.04	PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo]	[more]
XP_023552711.1	6.0e-156	84.32	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo ...	[more]

BLAST of Spg021283 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	3.8e-68	45.67	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	6.4e-68	45.67	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	6.9e-62	44.07	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	5.1e-57	43.30	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	3.9e-41	39.35	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of Spg021283 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UCR1	3.4e-157	85.09	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1EB90	4.5e-157	84.91	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]
A0A1S3BLF4	5.9e-157	85.04	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1CWG8	8.5e-156	87.06	short-chain dehydrogenase TIC 32, chloroplastic isoform X2 OS=Momordica charanti...	[more]
A0A6J1HN85	2.5e-155	84.32	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]

BLAST of Spg021283 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	8.2e-119	68.28	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	2.5e-115	66.47	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	1.2e-80	51.20	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	4.7e-74	49.24	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	3.5e-69	53.96	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 116..127 score: 42.55 coord: 37..54 score: 44.95 coord: 233..250 score: 36.72 coord: 166..182 score: 27.32
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..180 e-value: 3.0E-26 score: 92.1
None	No IPR available	GENE3D	3.40.50.720		coord: 23..333 e-value: 1.6E-70 score: 239.5
None	No IPR available	PANTHER	PTHR24320:SF210	MITOCHONDRIAL PYRUVATE CARRIER	coord: 4..334
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..334
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 37..323 e-value: 9.674E-91 score: 270.635
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..303

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021283.1	Spg021283.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity