Spg021248 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021248
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	vacuolar protein sorting-associated protein 25
Location	scaffold6: 47238167 .. 47243200 (-)
RNA-Seq Expression	Spg021248
Synteny	Spg021248

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATTCAAACCTCTGACCTCATGGTCGAGTGTACATGCCAATTACCGCTGAGCTTTGACATAGTTTTACCTATTTTTAAAAAGAAAAAAAAGTATTATAAAAAAAAGAAAAAGAAAAAAGGTTATTTGAAAATAAAAAAAGAAAAAAAGAAAAAAAGGTAAAAGTGAAGGCTAACAGGCCGACGAGATTCGTATATTGGGCCGGAAGTGGGCTCGAGTTTGCACTTGCGAACCGGACTATATCGTCTTGGTTAGCCGATTGAGGAAGCCGAAATTCGATTTTGTTCTTCATTTTTCGAATCGAAATTCGATACTTGCGTGCCATTCACGATTGATCGATCACAGGTACAATTCATTTTTCCTTTCCTGTCTCTAAATTTTCATTTCGCTTCCACTGTTTGATCTCAATATCGTAACACATCAATTGAAAATCATGACAACACCGCGGTTAAGGGGAATTTTGAAAGATTTTTGTATCTCTGCATCGTTTGAATACTTAATTTTCAAGTTTGGAGTTCTCGATTCTCAGTTTGTTGCTGTTGTTTTTCCCCTGAAATCAAGTGATCGACTGTTCCGAACGTGGCTGCATATGTTCAGTTCTAAACTTCCAACTTCGAAAATTTCAGCTACCAAATCGCGTTTTGACCGTGCTTCGTTGGTTATCTGTCTTCATATCCATTTTCAAGCATTAGATCGTAGATAATGGTTCTCTCACTAAAGAAAGGATGAGTTTAGGATGAATTTTGAAAATATTCGTAGCGTTCTGACCATTTTGTTACTCTTTTCATTCTTCTCAATATCTCCCTCTCTACAACCCACGTCGGCACGCCGGCACGCCGGCAGACCTCTTTCGGCAACGCCCATTTCTTTCTTCTCTCCCTATCTCGCTCTCTAATGCAACTGCGCCAGCTCACACGATTGCCCAACCTTCTGCTCGTCGCCGATTGGCCACTGCATTCTTCTACTCGTCGCAGGGATTAATTTTTTTTTCCTTATTTTTTCCTGTTTATTTTGCTTTCTTCTATTCAGACAATTCCAAAATATGGTAAAATTGGAATGAGGTCAAATTTAATCTAAAATCCTGCCTAATCTGTCCAATTTTTTTTGGACAAAGTGGGGTTTCAAACCCTTTTTTTTTTAATTCAAACCAAACCCTGGAAATAAATACAATTCCTTTCACATTTGGGGGTTCCAAACAGAGGGTAAAGTATTGTTTAGGAATAGTTGATTAGTTTCTTCCAGAAGTGTTCTTAAGAGCACTTTTTTTGTAATTCCAAACTCTCCTGAAGTCATCATGTCTGTTTATTTCCAGTGAAGTGCTGTTCTGTCTCGGTTAATTTCATAAGCCTTTTTCTTTTAGCCTTGTAGGCAGTAGCATACGAGTTATGAAGTTGTTCAGTGTTTCTTCAACAGTTCTGCTTGGTCAAGTAGCTGGTTAATTACACATTGACTATAAAAACGCACAAAAGGTGCTCAATCATTAATATCATTCAAGATATTTCCAACGAGGCCTCCTAACTTCATACCATGGTTATTTCAGTCTGAGATTTTTTGGTTGTGAAGTTTTCATGCCATACGGTGTTTTATAGCTATAGTTTCACCTTATATCAAGTTTATTTTTCAGTTCTCACCATTGATATGATTCATAAAAACACAGAAACTTGATTCTACTTTGACATGCTCATTTTCTATTACAATCATCCACAGGATGAAAATTAGAAAAGGTGCACAACTTGTTTTCCCGTTCAAGTTTACATTGTTTTGTAATGATAAGCTCGTATTAGTTTAGTTTGCAAGTCTCTATTTATTTTTTTATTTTTAGGGGTGGGAGATTCAAACCTTTGAGCTATGTTCATATCTTAACTAGTTGAGCTATGTTCAGGTTGGCTAGTTTACAAGTATCTAGTCTCATCAAATTATTAACAACTCAAAAATAGTTATGCATTGACAGAAAATGGCTAGGTTTGTCATGTTTATGAATTACTGGTTATTTTCTTTCTTCCCATTCCTCTGAATTTCTCAGTTCTATAGAAAAGATGCAGCCATAGGATTCACTTACAAAGGAAGCAATAAAGAAAATGCAGAAATTGGGGGATTTCAAGTTACCACATTTCTTCAATTATCCACCATATTTCACGTAATTTTCCTACTCTGTCTTAGTTACCGTTGTTTTACGTCATTATAAGCTACAACAAGTAACTTGGAAGTTGTCTTTGTCAATTTCTGTTGATAATGATATCTTGGTTAGAATGGCTGTTTTATGAGTGAATGAATGAAACTTACACTACATTACTCAACTAGTATTTACGTAACACTAACAGGATACTGGATCACTTTACTTTCCCCATAAGCCTCATAAATATGAACGTAATAATGCTATCTTGCTGCAATACCTGCAATTGTTATCGATAGCTTTGGTTGTTTCACTTTTTGCAAGCTTTTCTTTTGTCTTGAGTTGCATATGTAACCTTTTATTGCATGTCTTCTCAGTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAGCTCTGGAAGGAACTAATTCTCGACTATTGCCGAACACAAAAGATATTTGTCATTGGACTCGAAGAAGAGTTTCCTCTATTTGCAAATTCTGTCATTGAGAGTAAATTCATGAACTTGTTTGGTCCTAATTGGTTGATATAATATAACCTATTTATAAATCATTCATGCATAAATATCAACCTTAGAAAGGCCATTGACATTAGCATCATGAACAGTGTGAAAAAGATTAAGGCATATCATTTTATGCAACCTTTTCACTCTACGAAGCATGGCATATCATTGGGATTGGTGTTTAGGACTCCAGTTTTTTGATGTATTTTACAGCGTGGTTGTGATGCTACAGGATCTCTTAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTTAGGCCCTGCTAGTTTAGTTCTATAATTCTATTTGTTTACAGTTTCCATTCATTCCCGAGGGAAAAACTGATATTTGGTACTTTAATTGTCCAATAGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGACTAAATACTTGTTAACTTTCATAGTTATGTAAAAGTTGATTGGTTTAGGGTGGGTTTAGAAGGACTTTTGAAATGGTTAAAGTTGCTATTATCCCATTAAAAGCAATTTTCAAAATTCATATAATATTTATTTAGATAACCAAAAGTGCTTTGAAAAGTTCTTATTCTAAAACTATTGCCTTGTTGAATAGGTGAAAAATAATGGACTTGAAGATAGTGTTATGACAGTCGAGGAAATGCGTTCGGGAGTTGAATCTAGAGGAACAGGTACTTCCCTTGCTTCTACTGAGTTGATTTAGATCTTCAAACGATTTCTAAATTTATTTTTTCATATGCTACATTTTTTTAAGTACAACAAAGGTGTGGTAAGGAGATTTGACCCTCCAACTTCAAGGAAAGAAGTATATATCAATTACGATTGAGTTATGCTCGCGTTGACTTTCATATACTACATTGATATTGGTTTTAAAGTGACTCTGGTAGGTTAACTATTAATTAAATTAGGTGGGCGCAAAAGCATCTTGGTGCTTAATAAATTCAGGAAATATGTAGATTGGCTTAAAGGGAAACGAAACTCTGGCATAAACTGTTTATGTGAATGTATATCTTTTTAGTTTAAATCTCATTTAGACTTTTAAACTTCCAGACTTGTTCATATATGTCAACTTGAGTATAGTTCAACTGGTTAAGATATATATTATTGTTCAAGAGGTTAGAGGTTTAAATCCTCCCATCTCACATGTTATTGAACTCAAAAGAAAACTTGTTCTTATATTTTTTAAAATTTTCTACTTTGAATTCTTTGGTCCTTATCAAATCTGTAGATAATATTTCGCAAATTTGATGCTAAACCGAATGCATGAGACTTGAACATGAATTTAATTTATGTGATGTCATGTACGAATTTTGTGGAATGCTCTAAAAAGTTACATGCTAAAGACAAATAATTTTCTACGCAAAATTCATAAACCGAATTGGAACATTTGTCCAAGGACCAAAACAAATAGATGTAAACATTCTTGGACCACATTTTCTTCTTGAATGATGATATGTGCTTGCTGCTTGCGTTTGGTAATGATTTTGGAATGACAAAAATCACTTTTTTCATATCTTTTTAGTTATTTTGGACATGACAAAAGTGTTTTTAGTCATTTCAAAATACCTTTATGTTTGTGTTTAAATACTAGGTTAAAATACCATTTGGTCCCTATACGTCGAGTGTTATTCCATATTAGTCCCTGTACTTTCAAATGTCCAATTTTAGTCACTGTACTTTCAATAAAGTTTAAAATTAGTTCATACCATAAGTTTATTATTATTTAACCGATTTTGAAATTGGACATTTGAGAGTATAGTGACTAAAATGAAACAAAACTCAAAGTACCGGGACCAAGATGATATTTTAACCTAAATACTACAACATGCAATCACATCCAAACTATCTTCACAAACCAGTATATTTATTTTTCTTAAATGTCTGTTGTATGCATCAGAGCTTCAAGGGATTGATCGTACAATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGGAAGCTCGCAATTTTCAAGGGTTCTTCAACAGACGACGAAGGCATTAAATTCTCTGTATAACTACAATAATTATTCACATTGCCACTCATAGTTTTTGAACAGTCTCAATGTGAATTTTCCAGATCAATCTTCTAAATGCTACTTTTCTTTAGTTTCCCTGCCAATTCAAACTGGGACTGTAGTTTTGTTTTTGGCCAAGTTCTGGTGCCGTACTTCAGTAAGCCTTCAAATTACTTAAATTTTCTTTGGGAGTGCTTTAGAAGATATTTTGTCAAGGAATGTCCCCACCCACCAGGATGACTTCCCCGTTCCTGTCCCCATCCCCCATTTTAATCTTCAGCCTGCAAAATTTTCCGTTA

mRNA sequence

ATGCAGAAATTGGGGGATTTCAAGTTACCACATTTCTTCAATTATCCACCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAGCTCTGGAAGGAACTAATTCTCGACTATTGCCGAACACAAAAGATATTTGTCATTGGACTCGAAGAAGAGTTTCCTCTATTTGCAAATTCTGTCATTGAGAGATCTCTTAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTTATGACAGTCGAGGAAATGCGTTCGGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACAATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGGAAGCTCGCAATTTTCAAGGGTTCTTCAACAGACGACGAAGGCATTAAATTCTCTGTATAA

Coding sequence (CDS)

ATGCAGAAATTGGGGGATTTCAAGTTACCACATTTCTTCAATTATCCACCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAGCTCTGGAAGGAACTAATTCTCGACTATTGCCGAACACAAAAGATATTTGTCATTGGACTCGAAGAAGAGTTTCCTCTATTTGCAAATTCTGTCATTGAGAGATCTCTTAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTTATGACAGTCGAGGAAATGCGTTCGGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACAATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGGAAGCTCGCAATTTTCAAGGGTTCTTCAACAGACGACGAAGGCATTAAATTCTCTGTATAA

Protein sequence

MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLFANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV

Homology

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BLAST of Spg021248 vs. NCBI nr
Match: XP_022949655.1 (vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_023005573.1 vacuolar protein sorting-associated protein 25 [Cucurbita maxima] >KAG6596709.1 Vacuolar protein sorting-associated protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 359.0 bits (920), Expect = 2.4e-95
Identity = 175/179 (97.77%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. NCBI nr
Match: XP_008448627.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo] >KAA0053011.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa] >TYK11467.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 357.8 bits (917), Expect = 5.3e-95
Identity = 174/179 (97.21%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR+AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. NCBI nr
Match: XP_022145259.1 (vacuolar protein sorting-associated protein 25 [Momordica charantia])

HSP 1 Score: 357.8 bits (917), Expect = 5.3e-95
Identity = 174/179 (97.21%), Postives = 176/179 (98.32%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
            N+ IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEMRSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. NCBI nr
Match: XP_023540532.1 (vacuolar protein sorting-associated protein 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357.5 bits (916), Expect = 6.9e-95
Identity = 174/179 (97.21%), Postives = 177/179 (98.88%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR  FLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. NCBI nr
Match: XP_004146105.1 (vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hypothetical protein Csa_009640 [Cucumis sativus])

HSP 1 Score: 355.9 bits (912), Expect = 2.0e-94
Identity = 172/179 (96.09%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR+AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. ExPASy Swiss-Prot
Match: Q8VZC9 (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 GN=VPS25 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 1.5e-87
Identity = 152/179 (84.92%), Postives = 168/179 (93.85%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EE+FPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NS I+RSLSHEARE FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSG ES GTELQGIDRTILMRALK LE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of Spg021248 vs. ExPASy Swiss-Prot
Match: Q6AX45 (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps25 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 9.3e-34
Identity = 70/173 (40.46%), Postives = 105/173 (60.69%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPLFANSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  E PLF N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           I+R LS E+ +  L  L   G  EW+DK   + LI+W R  +W  +I  +V  NG+ +SV
Sbjct: 63  IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSV 122

Query: 127 MTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 123 FTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

[Close]

BLAST of Spg021248 vs. ExPASy Swiss-Prot
Match: Q6NWF4 (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 PE=2 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 1.0e-32
Identity = 69/173 (39.88%), Postives = 106/173 (61.27%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPLFANSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  E P+F N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           IER LS EA +     L   G  EWLDK   +CLI+W R ++W  +I  +V  NG+ +SV
Sbjct: 63  IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSV 122

Query: 127 MTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 123 FTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

[Close]

BLAST of Spg021248 vs. ExPASy Swiss-Prot
Match: Q55GD9 (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44689 GN=vps25 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 67/188 (35.64%), Postives = 114/188 (60.64%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFP----LFAN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       LF N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  SVIERSLSHEAREAFLSALVSDGRAEWLDK-------------GHRKCLILWHRIQDWAD 126
             I R LS EA ++ +  ++ +G AEW+DK              + + LI+W +  +WA 
Sbjct: 66  EKINRKLSREALKSIIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWAS 125

Query: 127 IILHFVKNNGLEDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSS 178
           +I  +V + GL ++V+TV E+++G +S+  E   ++ TILM++LK LE++ K   F  S 
Sbjct: 126 LIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQ 185

[Close]

BLAST of Spg021248 vs. ExPASy Swiss-Prot
Match: Q9CQ80 (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.6e-28
Identity = 65/173 (37.57%), Postives = 98/173 (56.65%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCR--TQKIFVIGLEEEFPLFANSV 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR   Q    +   +E PLF N  
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           ++R L  E+ +  L  L   G  EWLDK     LI+W R ++W  +I  +V  +G  +SV
Sbjct: 65  LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSV 124

Query: 127 MTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 125 FTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

[Close]

BLAST of Spg021248 vs. ExPASy TrEMBL
Match: A0A6J1L2J9 (vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LOC111498518 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.2e-95
Identity = 175/179 (97.77%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. ExPASy TrEMBL
Match: A0A6J1GDD3 (vacuolar protein sorting-associated protein 25 OS=Cucurbita moschata OX=3662 GN=LOC111452977 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.2e-95
Identity = 175/179 (97.77%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. ExPASy TrEMBL
Match: A0A5A7UHJ5 (Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001240 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.6e-95
Identity = 174/179 (97.21%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR+AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. ExPASy TrEMBL
Match: A0A6J1CUP8 (vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN=LOC111014756 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.6e-95
Identity = 174/179 (97.21%), Postives = 176/179 (98.32%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
            N+ IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEMRSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. ExPASy TrEMBL
Match: A0A1S3BKR8 (vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490745 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.6e-95
Identity = 174/179 (97.21%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR+AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg021248 vs. TAIR 10
Match: AT4G19003.2 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 323.6 bits (828), Expect = 1.0e-88
Identity = 152/179 (84.92%), Postives = 168/179 (93.85%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EE+FPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NS I+RSLSHEARE FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSG ES GTELQGIDRTILMRALK LE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of Spg021248 vs. TAIR 10
Match: AT4G19003.1 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 323.6 bits (828), Expect = 1.0e-88
Identity = 152/179 (84.92%), Postives = 168/179 (93.85%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EE+FPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NS I+RSLSHEARE FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEE+RSG ES GTELQGIDRTILMRALK LE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

The following BLAST results are available for this feature:

BLAST of Spg021248 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949655.1	2.4e-95	97.77	vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_02300557...	[more]
XP_008448627.1	5.3e-95	97.21	PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me...	[more]
XP_022145259.1	5.3e-95	97.21	vacuolar protein sorting-associated protein 25 [Momordica charantia]	[more]
XP_023540532.1	6.9e-95	97.21	vacuolar protein sorting-associated protein 25 [Cucurbita pepo subsp. pepo]	[more]
XP_004146105.1	2.0e-94	96.09	vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hyp...	[more]

BLAST of Spg021248 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZC9	1.5e-87	84.92	Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q6AX45	9.3e-34	40.46	Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps2...	[more]
Q6NWF4	1.0e-32	39.88	Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 P...	[more]
Q55GD9	1.1e-29	35.64	Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44...	[more]
Q9CQ80	2.6e-28	37.57	Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25...	[more]

BLAST of Spg021248 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L2J9	1.2e-95	97.77	vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GDD3	1.2e-95	97.77	vacuolar protein sorting-associated protein 25 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A5A7UHJ5	2.6e-95	97.21	Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. m...	[more]
A0A6J1CUP8	2.6e-95	97.21	vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN...	[more]
A0A1S3BKR8	2.6e-95	97.21	vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=365...	[more]

BLAST of Spg021248 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19003.2	1.0e-88	84.92	E2F/DP family winged-helix DNA-binding domain	[more]
AT4G19003.1	1.0e-88	84.92	E2F/DP family winged-helix DNA-binding domain	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 103..178 e-value: 1.1E-24 score: 87.9
IPR014041	ESCRT-II complex, Vps25 subunit, N-terminal winged helix	GENE3D	1.10.10.570		coord: 5..101 e-value: 4.3E-31 score: 109.2
IPR008570	ESCRT-II complex, Vps25 subunit	PFAM	PF05871	ESCRT-II	coord: 13..145 e-value: 7.0E-43 score: 146.2
IPR008570	ESCRT-II complex, Vps25 subunit	PANTHER	PTHR13149	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25	coord: 1..179
None	No IPR available	PANTHER	PTHR13149:SF1	SUBFAMILY NOT NAMED	coord: 1..179
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 102..177
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 7..107

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021248.1	Spg021248.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071985	multivesicular body sorting pathway
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component	GO:0000814	ESCRT II complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0005198	structural molecule activity