Spg021172 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg021172
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionBOI-related E3 ubiquitin-protein ligase 1-like
Locationscaffold6: 45830471 .. 45831877 (-)
RNA-Seq ExpressionSpg021172
SyntenySpg021172
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGCTTCCATAAAATCAATCCTCCATTTCTCTCTCTTCTCTCTCTCTCTCTCTCATTGATTCATTTCAATGGCTGTTCAAGCTCAATACCCTTCCAATGTCCTTCTCCTCAACAGGTTATTCCCTAAATTATCCTCTGTTTTTCATCTGTTTCTCTTCTCAATCTCTTTTGTTTGTTGATTTTTCTCTTGAATTTGCAGAAACATCCAAGATTCTGACCATGATTATTCATTGCAACCTCAGCCTGGTGGAGGAGGAGGAGTTCTTCAACAGTCTCATCTTCTGTTCAACAATGGAGGTTAGATTCAATTTTAGTTTTGGTTTTGGTGTTTTTGGATTGACCCATTGGAATTGAAGGGATTTTGATGATGGGTTTTGATTTTGGAAACCAGGTGGGAATCATTTGAGTAATTTGAGGAAAAGAGGGAGAGATGTCTCTGTTTCTGTCTCTGTTTCAGCTCCCTTAATCAATCCTCTCTGTTTTCAGCCTCAGATTATTGATGTTTCTCAGCTTCATAACCACCACCACCCTTCCAATGTCGTCTCCACCGGCCTCCGGTTGTCCTCCGGCGACCAACCCAATAGCCTCCACCACCATCCGCCGCCGCCGCCGCCGCAGCAGGACGATACAAATCTCGTTTCTCTCTCCTCCTCTGTTTTCTTCTCCGATGACTTTTCGTCTCAAATCAAACAGCACCGTGATGAAATTGACCAATTCCTTCAAGCCCAGGTACGGATTTGAGAAACCCAGATGGTATTTTGGAGGAAAGAGAAGAAAAGGTTTGATTTTGTTTAAATTTTGAGTTTTTTTTTTTTGGGTTATGATTAGGAGGAGGAGCTGCGGCGGACGTTGGCGGAGAAACGGCAGAGGCACTACCGGGAACTGCTGGGGGTGGCGGAGGAGACGGCGGCGAGGAGGTTGAGGGAGAAGGAGGTGGAGGTGGAGAAGGCCACGCGCCGCCACGCTGAGTTGGAAGCACGCGCTGCCAGGCTGACCATGGAGGCGCAGGCGTGGCAGGCGAAGGCCAGGGCGGAGGAGGCGGCGGCGGCGGCTCTGCAGGCCCAATTGCAGCAGGCGATAATGAGCGGCGCTGGAATTGGCGGCAGCGGCGACGGCGGAGGAGACTGCACGGCGGCGGCGGCAGAGGATGCCGAGTCGGGTTTTGTTGACCCGGGCCGAGTCGCCACCGAGTTTGGACCGAGTTGCAAAGCGTGTCGGAAGCGAGTCGCGTCGGTGGTTTTGCTGCCGTGTCGGCATTTGTGCGTTTGTACGGATTGCGATCACGTGGTTCGCACGTGCCCGCTTTGTCGGGCCCTGAGGAATTCCAGTGTTGAAGTTTATCTCTCTTGAGGCCCAATAAGGCCCAACGGCCCACATCTGACTGCCCCAGGCCCAATGGTAAAACCC

mRNA sequence

ATGGCTGTTCAAGCTCAATACCCTTCCAATGTCCTTCTCCTCAACAGAAACATCCAAGATTCTGACCATGATTATTCATTGCAACCTCAGCCTGGTGGAGGAGGAGGAGTTCTTCAACAGTCTCATCTTCTGTTCAACAATGGAGGTGGGAATCATTTGAGTAATTTGAGGAAAAGAGGGAGAGATGTCTCTGTTTCTGTCTCTGTTTCAGCTCCCTTAATCAATCCTCTCTGTTTTCAGCCTCAGATTATTGATGTTTCTCAGCTTCATAACCACCACCACCCTTCCAATGTCGTCTCCACCGGCCTCCGGTTGTCCTCCGGCGACCAACCCAATAGCCTCCACCACCATCCGCCGCCGCCGCCGCCGCAGCAGGACGATACAAATCTCGTTTCTCTCTCCTCCTCTGTTTTCTTCTCCGATGACTTTTCGTCTCAAATCAAACAGCACCGTGATGAAATTGACCAATTCCTTCAAGCCCAGGAGGAGGAGCTGCGGCGGACGTTGGCGGAGAAACGGCAGAGGCACTACCGGGAACTGCTGGGGGTGGCGGAGGAGACGGCGGCGAGGAGGTTGAGGGAGAAGGAGGTGGAGGTGGAGAAGGCCACGCGCCGCCACGCTGAGTTGGAAGCACGCGCTGCCAGGCTGACCATGGAGGCGCAGGCGTGGCAGGCGAAGGCCAGGGCGGAGGAGGCGGCGGCGGCGGCTCTGCAGGCCCAATTGCAGCAGGCGATAATGAGCGGCGCTGGAATTGGCGGCAGCGGCGACGGCGGAGGAGACTGCACGGCGGCGGCGGCAGAGGATGCCGAGTCGGGTTTTGTTGACCCGGGCCGAGTCGCCACCGAGTTTGGACCGAGTTGCAAAGCGTGTCGGAAGCGAGTCGCGTCGGTGGTTTTGCTGCCGTGTCGGCATTTGTGCGTTTGTACGGATTGCGATCACGTGGTTCGCACGTGCCCGCTTTGTCGGGCCCTGAGGAATTCCAGTGTTGAAGTTTATCTCTCTTGA

Coding sequence (CDS)

ATGGCTGTTCAAGCTCAATACCCTTCCAATGTCCTTCTCCTCAACAGAAACATCCAAGATTCTGACCATGATTATTCATTGCAACCTCAGCCTGGTGGAGGAGGAGGAGTTCTTCAACAGTCTCATCTTCTGTTCAACAATGGAGGTGGGAATCATTTGAGTAATTTGAGGAAAAGAGGGAGAGATGTCTCTGTTTCTGTCTCTGTTTCAGCTCCCTTAATCAATCCTCTCTGTTTTCAGCCTCAGATTATTGATGTTTCTCAGCTTCATAACCACCACCACCCTTCCAATGTCGTCTCCACCGGCCTCCGGTTGTCCTCCGGCGACCAACCCAATAGCCTCCACCACCATCCGCCGCCGCCGCCGCCGCAGCAGGACGATACAAATCTCGTTTCTCTCTCCTCCTCTGTTTTCTTCTCCGATGACTTTTCGTCTCAAATCAAACAGCACCGTGATGAAATTGACCAATTCCTTCAAGCCCAGGAGGAGGAGCTGCGGCGGACGTTGGCGGAGAAACGGCAGAGGCACTACCGGGAACTGCTGGGGGTGGCGGAGGAGACGGCGGCGAGGAGGTTGAGGGAGAAGGAGGTGGAGGTGGAGAAGGCCACGCGCCGCCACGCTGAGTTGGAAGCACGCGCTGCCAGGCTGACCATGGAGGCGCAGGCGTGGCAGGCGAAGGCCAGGGCGGAGGAGGCGGCGGCGGCGGCTCTGCAGGCCCAATTGCAGCAGGCGATAATGAGCGGCGCTGGAATTGGCGGCAGCGGCGACGGCGGAGGAGACTGCACGGCGGCGGCGGCAGAGGATGCCGAGTCGGGTTTTGTTGACCCGGGCCGAGTCGCCACCGAGTTTGGACCGAGTTGCAAAGCGTGTCGGAAGCGAGTCGCGTCGGTGGTTTTGCTGCCGTGTCGGCATTTGTGCGTTTGTACGGATTGCGATCACGTGGTTCGCACGTGCCCGCTTTGTCGGGCCCTGAGGAATTCCAGTGTTGAAGTTTATCTCTCTTGA

Protein sequence

MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGGVLQQSHLLFNNGGGNHLSNLRKRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHHHPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHYRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQLQQAIMSGAGIGGSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVASVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS
Homology
BLAST of Spg021172 vs. NCBI nr
Match: XP_004147249.1 (BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN55804.1 hypothetical protein Csa_010249 [Cucumis sativus])

HSP 1 Score: 540.0 bits (1390), Expect = 1.4e-149
Identity = 289/336 (86.01%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGGVLQQSHLLFNNGGGNHLSNLRKRG 60
           MAVQAQYPSNVLLLNRNIQD++HDYSLQPQPGGGGG+LQQSH LFNNGGGNHLSNLRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNIQDAEHDYSLQPQPGGGGGILQQSHALFNNGGGNHLSNLRKRG 60

Query: 61  RDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHHHPPP 120
           RD   SVSVS P INPLCFQPQIID+SQLHNHHHPSNVVSTGLRLSSGDQP +L+HHPPP
Sbjct: 61  RD--NSVSVSPPTINPLCFQPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQPLNLYHHPPP 120

Query: 121 PPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHYREL 180
           PP  Q+  +LVSLSSSVF SDDFSSQIKQHR+EIDQFLQ QEEELRRTLAEKRQRHYREL
Sbjct: 121 PPSSQNHASLVSLSSSVFISDDFSSQIKQHREEIDQFLQTQEEELRRTLAEKRQRHYREL 180

Query: 181 LGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQ 240
           L  AEE A RRLREKEVEVEKATRRHAELEARAARL+MEA AWQ KARAEEAAAAALQAQ
Sbjct: 181 LAAAEERAVRRLREKEVEVEKATRRHAELEARAARLSMEAAAWQEKARAEEAAAAALQAQ 240

Query: 241 LQQAIMSGAGIGGSGDGG--GDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVASVV 300
           LQQAIM G GIGGSGDGG  GDCT   AEDAESG++DP RV  E GPSCK+CRKRVASVV
Sbjct: 241 LQQAIMRGTGIGGSGDGGVVGDCT---AEDAESGYIDPERVLAESGPSCKSCRKRVASVV 300

Query: 301 LLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           LLPCRH CVC+ CDHVVRTCPLCRA RNSSVEVYLS
Sbjct: 301 LLPCRHFCVCSVCDHVVRTCPLCRASRNSSVEVYLS 331

BLAST of Spg021172 vs. NCBI nr
Match: XP_008448752.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo])

HSP 1 Score: 528.5 bits (1360), Expect = 4.2e-146
Identity = 287/339 (84.66%), Postives = 303/339 (89.38%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQP--GGGGGVLQQSHLLFNNGGGNHL-SNLR 60
           MAVQAQYPSNVLLLNRNIQD++HDYSLQPQP  GGGGG+LQQSH LFNNGGGNHL SNLR
Sbjct: 1   MAVQAQYPSNVLLLNRNIQDAEHDYSLQPQPGGGGGGGILQQSHALFNNGGGNHLSSNLR 60

Query: 61  KRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHHH 120
           KRGRD   SVSVS P INPLCFQPQIID+SQLHNHHHPSNVVSTGLRL SGDQP +L+HH
Sbjct: 61  KRGRD--NSVSVSPPTINPLCFQPQIIDLSQLHNHHHPSNVVSTGLRLFSGDQPLNLYHH 120

Query: 121 PPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHY 180
           PPPPP  Q+D +LVSLSSSVF SDDFSSQIKQHR+EIDQFLQ QEEELRR LAEKRQRHY
Sbjct: 121 PPPPPSSQNDASLVSLSSSVFVSDDFSSQIKQHREEIDQFLQTQEEELRRALAEKRQRHY 180

Query: 181 RELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAAL 240
           RE+L  AEE AARRLREKEVEVEKATRRHAELEARAARL+MEA AWQ KARAEEAAAAAL
Sbjct: 181 REILAAAEERAARRLREKEVEVEKATRRHAELEARAARLSMEAAAWQEKARAEEAAAAAL 240

Query: 241 QAQLQQAIMSGAGIGGSGDGG--GDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVA 300
           QAQLQQAIM G GIGGSGDGG  GDCT   AEDAESG++DP RV  E GPSCK+CRKRVA
Sbjct: 241 QAQLQQAIMRGTGIGGSGDGGVVGDCT---AEDAESGYIDPDRVIAESGPSCKSCRKRVA 300

Query: 301 SVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           SVVLLPCRH C+C+ CDHVVRTCPLCRA RNSSVEVYLS
Sbjct: 301 SVVLLPCRHFCLCSVCDHVVRTCPLCRASRNSSVEVYLS 334

BLAST of Spg021172 vs. NCBI nr
Match: XP_023005462.1 (E3 ubiquitin-protein ligase BOI-like [Cucurbita maxima])

HSP 1 Score: 519.2 bits (1336), Expect = 2.6e-143
Identity = 281/338 (83.14%), Postives = 298/338 (88.17%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGG----VLQQSHLLFNNGGGNHLSNL 60
           MAVQAQYPSNVL LNRNIQDS+HDYSLQPQPGGGGG    V QQSH LFNNGGGNH +NL
Sbjct: 1   MAVQAQYPSNVLFLNRNIQDSEHDYSLQPQPGGGGGGGGDVFQQSHHLFNNGGGNHFNNL 60

Query: 61  RKRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHH 120
           RKRGRDVSVS+SVS PLINPLCF PQIID+SQLHNH+HPSNVVSTGLRLSS DQ  +LH+
Sbjct: 61  RKRGRDVSVSLSVSPPLINPLCFHPQIIDLSQLHNHYHPSNVVSTGLRLSSDDQQPNLHY 120

Query: 121 HPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRH 180
           H  PPPP ++DTNLVS SSSVF SDDFSSQ +Q RDEID+FLQ QEEELRR LAEKRQRH
Sbjct: 121 H--PPPPARNDTNLVSFSSSVFLSDDFSSQTRQQRDEIDRFLQTQEEELRRALAEKRQRH 180

Query: 181 YRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAA 240
           YRELLG AEET ARRL EKE+EVEKATRRHAELEARAARL +EAQAWQAKA+AEEAAAAA
Sbjct: 181 YRELLGAAEETVARRLLEKEMEVEKATRRHAELEARAARLCVEAQAWQAKAKAEEAAAAA 240

Query: 241 LQAQLQQAIMSGAGIGGSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVAS 300
           LQAQLQQAI  GAGI   G+GGGDCT AAAEDA S +VDP RV TEFGPSCK C KRVAS
Sbjct: 241 LQAQLQQAITRGAGISSGGEGGGDCT-AAAEDAGSCYVDPERVGTEFGPSCKVCWKRVAS 300

Query: 301 VVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           VVLLPCRHLCVCTDCD VVRTCPLC A+RNSSVEVYLS
Sbjct: 301 VVLLPCRHLCVCTDCDQVVRTCPLCPAVRNSSVEVYLS 335

BLAST of Spg021172 vs. NCBI nr
Match: XP_023539664.1 (E3 ubiquitin-protein ligase BOI-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 512.7 bits (1319), Expect = 2.4e-141
Identity = 281/349 (80.52%), Postives = 297/349 (85.10%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGG----------VLQQSHLLFNNGGG 60
           MAVQAQYPSNVL LNRNIQDS+HDYSLQPQPGGGGG          V QQSH LFNNGGG
Sbjct: 1   MAVQAQYPSNVLFLNRNIQDSEHDYSLQPQPGGGGGGGGGGGGGGDVFQQSHHLFNNGGG 60

Query: 61  NHLSNLRKRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHN----HHHPSNVVSTGLRLS 120
           NH +NLRKRGRDVSVS+SVS P INPLCF PQIID SQLHN    HHHPSNVVSTGLRLS
Sbjct: 61  NHFNNLRKRGRDVSVSLSVSPPFINPLCFHPQIIDFSQLHNHHHHHHHPSNVVSTGLRLS 120

Query: 121 SGDQPNSLHHHPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELR 180
           S DQ  +LHHH  PPPP ++DTNLVSLSSSVF S+DFSSQ +Q RDEID+FLQ QEEELR
Sbjct: 121 SDDQQPNLHHH--PPPPARNDTNLVSLSSSVFLSEDFSSQTRQQRDEIDRFLQTQEEELR 180

Query: 181 RTLAEKRQRHYRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAK 240
           R LAEKRQRHYRELLG AEET ARRL EKE+EVEKATRRHAELEARAARL +EAQAWQAK
Sbjct: 181 RALAEKRQRHYRELLGAAEETVARRLLEKEMEVEKATRRHAELEARAARLCVEAQAWQAK 240

Query: 241 ARAEEAAAAALQAQLQQAIMSGAGIGGSGDGGGDC-TAAAAEDAESGFVDPGRVATEFGP 300
           A+AEEAAAAALQAQLQQAIM GAGI   G+GGGDC  AAAAEDA S +VDP R+  EFGP
Sbjct: 241 AKAEEAAAAALQAQLQQAIMRGAGISSGGEGGGDCMAAAAAEDAGSCYVDPERIGAEFGP 300

Query: 301 SCKACRKRVASVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           SCK C KRVASVVLLPCRHLCVCTDCD VVRTCPLC A+RNSSVEVYLS
Sbjct: 301 SCKVCWKRVASVVLLPCRHLCVCTDCDQVVRTCPLCPAVRNSSVEVYLS 347

BLAST of Spg021172 vs. NCBI nr
Match: XP_022965101.1 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 2.4e-141
Identity = 283/338 (83.73%), Postives = 294/338 (86.98%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGGV----LQQSHLLFNNGGGNHLSNL 60
           MAVQAQYPSNVLL NRNIQDS HDYSLQPQPGGGGGV    L+QSH LFNNGGGN+LSNL
Sbjct: 1   MAVQAQYPSNVLLHNRNIQDSGHDYSLQPQPGGGGGVGGGDLEQSHALFNNGGGNYLSNL 60

Query: 61  RKRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHH 120
           RKRGRD    VSVSAP+INPLCFQPQIID+SQLHNHHHPSNVVSTGLRLSSGDQ  + HH
Sbjct: 61  RKRGRD----VSVSAPIINPLCFQPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQGLNPHH 120

Query: 121 HPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRH 180
           HPPP    Q+D NLVSLSSSVF SD+FSSQIK  RDEIDQFLQ+QEEELRR LAEKR RH
Sbjct: 121 HPPP----QNDDNLVSLSSSVFISDEFSSQIKVQRDEIDQFLQSQEEELRRALAEKRHRH 180

Query: 181 YRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAA 240
           YRELLG  E TA RRLREKEVEVEKATRRHAELEARAARL+MEAQAWQ KARAEEAAAA 
Sbjct: 181 YRELLGAMEATAVRRLREKEVEVEKATRRHAELEARAARLSMEAQAWQEKARAEEAAAAT 240

Query: 241 LQAQLQQAIMSGAGIGGSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVAS 300
           LQAQLQQAIM GAGIG    GGGDCT   AEDAESG+VDP RV  E GPSCKACRKRVAS
Sbjct: 241 LQAQLQQAIMKGAGIG----GGGDCT---AEDAESGYVDPDRVIAESGPSCKACRKRVAS 300

Query: 301 VVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           VVLLPCRH C+CT CDHVVRTCPLCRALRNSSVEVYLS
Sbjct: 301 VVLLPCRHFCICTVCDHVVRTCPLCRALRNSSVEVYLS 323

BLAST of Spg021172 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-14
Identity = 67/208 (32.21%), Postives = 106/208 (50.96%), Query Frame = 0

Query: 131 VSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHYRELLGVAEETAAR 190
           ++ S +     +  SQI+Q   EID+F+  Q E LR  L  +++   R L    +    +
Sbjct: 90  IAFSPASLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILK 149

Query: 191 RLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQLQQAIMSGAG 250
           +L+ K+ E+ +  + +  L+ R   L +E Q W+  A+  EA A  L++ L+Q +     
Sbjct: 150 KLKAKDEEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDD 209

Query: 251 IGGSGDGGGDCTAAAAEDAES--GFVDPGRVATEFGPSCKACRKRVASVVLLPCRHLCVC 310
           +    D         A+DAES  G  D G V       CK C +  ASV++LPCRHLC+C
Sbjct: 210 L----DAFRRPLVEEADDAESSCGSCDGGDVTAVVNGGCKRCGELTASVLVLPCRHLCLC 269

Query: 311 TDC--DHVVRTCPLCRALRNSSVEVYLS 335
           T C    ++RTCP+C  +  +SV V +S
Sbjct: 270 TVCGSSALLRTCPVCDMVMTASVHVNMS 293

BLAST of Spg021172 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.4e-14
Identity = 70/209 (33.49%), Postives = 107/209 (51.20%), Query Frame = 0

Query: 134 SSSVFFSDDFSSQIK-QHRDEIDQFLQAQEEELRRTLAEKRQRHYRELLGVAEETAARRL 193
           SS    + +  SQI+ Q + EID+F+  Q E+LR  +  ++Q   R L    +   A++L
Sbjct: 102 SSPSLINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKL 161

Query: 194 REKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQLQQAIMSGAGIG 253
           +EK+ E+ +    +  L+ R   L +E Q W+  A+  EA A  L+  L Q +       
Sbjct: 162 KEKDDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVL------- 221

Query: 254 GSGDGGGDCTAAAAEDAESGFVD-----PGRVATEFGPSCKACRKRVASVVLLPCRHLCV 313
              +     +A   +DAES          G   T  G  CK C +R ASV++LPCRHLC+
Sbjct: 222 AQLETFPTASAVVEDDAESSCGSCCGDGGGEAVTAVGGGCKRCGEREASVLVLPCRHLCL 281

Query: 314 CTDC--DHVVRTCPLCRALRNSSVEVYLS 335
           CT C    ++RTCP+C  + N+SV V +S
Sbjct: 282 CTVCGGSALLRTCPVCDMVMNASVHVNMS 303

BLAST of Spg021172 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.6e-13
Identity = 63/216 (29.17%), Postives = 104/216 (48.15%), Query Frame = 0

Query: 137 VFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHYRELLGVAEETAARRLREKE 196
           +F   D SS ++QH  +ID+ +    E +R  + EKR+   R ++   E+   + LR K+
Sbjct: 124 MFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKD 183

Query: 197 VEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQLQQAI----------- 256
            E+    + +  LE +   L +E Q W+  A++ EA   AL++ LQQ +           
Sbjct: 184 DEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEP 243

Query: 257 -------MSGAGIGGSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVASVV 316
                   S  G    GD   +    A E       D  ++       C++C K  ASV+
Sbjct: 244 PTVADDAQSCCGSNDEGDSEEERWKLAGEAQ-----DTKKMCRVGMSMCRSCGKGEASVL 303

Query: 317 LLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           LLPCRH+C+C+ C   + TCP+C++ + +S+ V LS
Sbjct: 304 LLPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

BLAST of Spg021172 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.4e-13
Identity = 80/261 (30.65%), Postives = 119/261 (45.59%), Query Frame = 0

Query: 104 RLSSGDQPNSLHHHPPPPPPQQDD-------TNLVSLSSSVFFSDDFSSQIKQHRDEIDQ 163
           R  S D   S +HH     P+          T   +L S +    D SS + Q + EID+
Sbjct: 99  RKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDR 158

Query: 164 FLQA---QEEELRRTLAEKRQRHYRELLGVAEETAARRLREKEVEVEKATRRHAELEARA 223
           F+     Q E ++  + EKR+R  R ++   E+   +RLR KE E E+  + +  LE R 
Sbjct: 159 FVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERV 218

Query: 224 ARLTMEAQAWQAKARAEEAAAAALQAQLQQA------IMSGAGI--------------GG 283
             L++E Q W+  A+  EA A  L+  L+        +  GAG+              G 
Sbjct: 219 KSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCGS 278

Query: 284 SGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVASVVLLPCRHLCVCTDCDH 335
           S  GGG+ T       E    D  +        C+ C +  + V+LLPCRHLC+C  C  
Sbjct: 279 SCGGGGEETVRRRVGLEREAQD--KAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGS 338

BLAST of Spg021172 vs. ExPASy Swiss-Prot
Match: O08863 (Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 5.2e-06
Identity = 22/48 (45.83%), Postives = 28/48 (58.33%), Query Frame = 0

Query: 287 CKACRKRVASVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           CK C  R  S+V +PC HL VC DC   +R CP+CR     +V  +LS
Sbjct: 553 CKVCMDREVSIVFIPCGHLVVCKDCAPSLRKCPICRGTIKGTVRTFLS 600

BLAST of Spg021172 vs. ExPASy TrEMBL
Match: A0A0A0L434 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G016980 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 6.8e-150
Identity = 289/336 (86.01%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGGVLQQSHLLFNNGGGNHLSNLRKRG 60
           MAVQAQYPSNVLLLNRNIQD++HDYSLQPQPGGGGG+LQQSH LFNNGGGNHLSNLRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNIQDAEHDYSLQPQPGGGGGILQQSHALFNNGGGNHLSNLRKRG 60

Query: 61  RDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHHHPPP 120
           RD   SVSVS P INPLCFQPQIID+SQLHNHHHPSNVVSTGLRLSSGDQP +L+HHPPP
Sbjct: 61  RD--NSVSVSPPTINPLCFQPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQPLNLYHHPPP 120

Query: 121 PPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHYREL 180
           PP  Q+  +LVSLSSSVF SDDFSSQIKQHR+EIDQFLQ QEEELRRTLAEKRQRHYREL
Sbjct: 121 PPSSQNHASLVSLSSSVFISDDFSSQIKQHREEIDQFLQTQEEELRRTLAEKRQRHYREL 180

Query: 181 LGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQ 240
           L  AEE A RRLREKEVEVEKATRRHAELEARAARL+MEA AWQ KARAEEAAAAALQAQ
Sbjct: 181 LAAAEERAVRRLREKEVEVEKATRRHAELEARAARLSMEAAAWQEKARAEEAAAAALQAQ 240

Query: 241 LQQAIMSGAGIGGSGDGG--GDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVASVV 300
           LQQAIM G GIGGSGDGG  GDCT   AEDAESG++DP RV  E GPSCK+CRKRVASVV
Sbjct: 241 LQQAIMRGTGIGGSGDGGVVGDCT---AEDAESGYIDPERVLAESGPSCKSCRKRVASVV 300

Query: 301 LLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           LLPCRH CVC+ CDHVVRTCPLCRA RNSSVEVYLS
Sbjct: 301 LLPCRHFCVCSVCDHVVRTCPLCRASRNSSVEVYLS 331

BLAST of Spg021172 vs. ExPASy TrEMBL
Match: A0A1S3BL24 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo OX=3656 GN=LOC103490824 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.1e-146
Identity = 287/339 (84.66%), Postives = 303/339 (89.38%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQP--GGGGGVLQQSHLLFNNGGGNHL-SNLR 60
           MAVQAQYPSNVLLLNRNIQD++HDYSLQPQP  GGGGG+LQQSH LFNNGGGNHL SNLR
Sbjct: 1   MAVQAQYPSNVLLLNRNIQDAEHDYSLQPQPGGGGGGGILQQSHALFNNGGGNHLSSNLR 60

Query: 61  KRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHHH 120
           KRGRD   SVSVS P INPLCFQPQIID+SQLHNHHHPSNVVSTGLRL SGDQP +L+HH
Sbjct: 61  KRGRD--NSVSVSPPTINPLCFQPQIIDLSQLHNHHHPSNVVSTGLRLFSGDQPLNLYHH 120

Query: 121 PPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHY 180
           PPPPP  Q+D +LVSLSSSVF SDDFSSQIKQHR+EIDQFLQ QEEELRR LAEKRQRHY
Sbjct: 121 PPPPPSSQNDASLVSLSSSVFVSDDFSSQIKQHREEIDQFLQTQEEELRRALAEKRQRHY 180

Query: 181 RELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAAL 240
           RE+L  AEE AARRLREKEVEVEKATRRHAELEARAARL+MEA AWQ KARAEEAAAAAL
Sbjct: 181 REILAAAEERAARRLREKEVEVEKATRRHAELEARAARLSMEAAAWQEKARAEEAAAAAL 240

Query: 241 QAQLQQAIMSGAGIGGSGDGG--GDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVA 300
           QAQLQQAIM G GIGGSGDGG  GDCT   AEDAESG++DP RV  E GPSCK+CRKRVA
Sbjct: 241 QAQLQQAIMRGTGIGGSGDGGVVGDCT---AEDAESGYIDPDRVIAESGPSCKSCRKRVA 300

Query: 301 SVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           SVVLLPCRH C+C+ CDHVVRTCPLCRA RNSSVEVYLS
Sbjct: 301 SVVLLPCRHFCLCSVCDHVVRTCPLCRASRNSSVEVYLS 334

BLAST of Spg021172 vs. ExPASy TrEMBL
Match: A0A6J1KT70 (E3 ubiquitin-protein ligase BOI-like OS=Cucurbita maxima OX=3661 GN=LOC111498441 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.2e-143
Identity = 281/338 (83.14%), Postives = 298/338 (88.17%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGG----VLQQSHLLFNNGGGNHLSNL 60
           MAVQAQYPSNVL LNRNIQDS+HDYSLQPQPGGGGG    V QQSH LFNNGGGNH +NL
Sbjct: 1   MAVQAQYPSNVLFLNRNIQDSEHDYSLQPQPGGGGGGGGDVFQQSHHLFNNGGGNHFNNL 60

Query: 61  RKRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHH 120
           RKRGRDVSVS+SVS PLINPLCF PQIID+SQLHNH+HPSNVVSTGLRLSS DQ  +LH+
Sbjct: 61  RKRGRDVSVSLSVSPPLINPLCFHPQIIDLSQLHNHYHPSNVVSTGLRLSSDDQQPNLHY 120

Query: 121 HPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRH 180
           H  PPPP ++DTNLVS SSSVF SDDFSSQ +Q RDEID+FLQ QEEELRR LAEKRQRH
Sbjct: 121 H--PPPPARNDTNLVSFSSSVFLSDDFSSQTRQQRDEIDRFLQTQEEELRRALAEKRQRH 180

Query: 181 YRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAA 240
           YRELLG AEET ARRL EKE+EVEKATRRHAELEARAARL +EAQAWQAKA+AEEAAAAA
Sbjct: 181 YRELLGAAEETVARRLLEKEMEVEKATRRHAELEARAARLCVEAQAWQAKAKAEEAAAAA 240

Query: 241 LQAQLQQAIMSGAGIGGSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVAS 300
           LQAQLQQAI  GAGI   G+GGGDCT AAAEDA S +VDP RV TEFGPSCK C KRVAS
Sbjct: 241 LQAQLQQAITRGAGISSGGEGGGDCT-AAAEDAGSCYVDPERVGTEFGPSCKVCWKRVAS 300

Query: 301 VVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           VVLLPCRHLCVCTDCD VVRTCPLC A+RNSSVEVYLS
Sbjct: 301 VVLLPCRHLCVCTDCDQVVRTCPLCPAVRNSSVEVYLS 335

BLAST of Spg021172 vs. ExPASy TrEMBL
Match: A0A6J1HKS9 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465067 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.2e-141
Identity = 283/338 (83.73%), Postives = 294/338 (86.98%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGGV----LQQSHLLFNNGGGNHLSNL 60
           MAVQAQYPSNVLL NRNIQDS HDYSLQPQPGGGGGV    L+QSH LFNNGGGN+LSNL
Sbjct: 1   MAVQAQYPSNVLLHNRNIQDSGHDYSLQPQPGGGGGVGGGDLEQSHALFNNGGGNYLSNL 60

Query: 61  RKRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHH 120
           RKRGRD    VSVSAP+INPLCFQPQIID+SQLHNHHHPSNVVSTGLRLSSGDQ  + HH
Sbjct: 61  RKRGRD----VSVSAPIINPLCFQPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQGLNPHH 120

Query: 121 HPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRH 180
           HPPP    Q+D NLVSLSSSVF SD+FSSQIK  RDEIDQFLQ+QEEELRR LAEKR RH
Sbjct: 121 HPPP----QNDDNLVSLSSSVFISDEFSSQIKVQRDEIDQFLQSQEEELRRALAEKRHRH 180

Query: 181 YRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAA 240
           YRELLG  E TA RRLREKEVEVEKATRRHAELEARAARL+MEAQAWQ KARAEEAAAA 
Sbjct: 181 YRELLGAMEATAVRRLREKEVEVEKATRRHAELEARAARLSMEAQAWQEKARAEEAAAAT 240

Query: 241 LQAQLQQAIMSGAGIGGSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVAS 300
           LQAQLQQAIM GAGIG    GGGDCT   AEDAESG+VDP RV  E GPSCKACRKRVAS
Sbjct: 241 LQAQLQQAIMKGAGIG----GGGDCT---AEDAESGYVDPDRVIAESGPSCKACRKRVAS 300

Query: 301 VVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           VVLLPCRH C+CT CDHVVRTCPLCRALRNSSVEVYLS
Sbjct: 301 VVLLPCRHFCICTVCDHVVRTCPLCRALRNSSVEVYLS 323

BLAST of Spg021172 vs. ExPASy TrEMBL
Match: A0A6J1GEA7 (E3 ubiquitin-protein ligase BOI-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453365 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.3e-140
Identity = 280/343 (81.63%), Postives = 294/343 (85.71%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGG------VLQQSHLLFNNGGGNHLS 60
           MAVQAQYPSNVL LNRNIQDS+HDYSLQPQPGGGGG      V QQSH LFNNGGGNH +
Sbjct: 1   MAVQAQYPSNVLFLNRNIQDSEHDYSLQPQPGGGGGGGGGEDVFQQSHHLFNNGGGNHFN 60

Query: 61  NLRKRGRDVSVSVSVSAPLINPLCFQPQIIDVSQLHN-HHHPSNVVSTGLRLSSGDQPNS 120
           NLRKRGRD    VSVS PLINPLCF PQIID SQLHN HHHPSNVVSTGLRLSS DQ  +
Sbjct: 61  NLRKRGRD----VSVSPPLINPLCFHPQIIDFSQLHNHHHHPSNVVSTGLRLSSDDQQPN 120

Query: 121 LHHHPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKR 180
           LHHH   PPP ++DTNLVSLSSSVF SDDFSSQ +Q RDEID+FLQ QEEELRR LAEKR
Sbjct: 121 LHHH---PPPARNDTNLVSLSSSVFLSDDFSSQTRQQRDEIDRFLQTQEEELRRALAEKR 180

Query: 181 QRHYRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAA 240
           QRHYRELLG AEET ARRL EKE+EVEKATRRHAELEARAARL +EAQAWQAKA+AEEAA
Sbjct: 181 QRHYRELLGAAEETVARRLLEKEMEVEKATRRHAELEARAARLCVEAQAWQAKAKAEEAA 240

Query: 241 AAALQAQLQQAIMSGAGIGGSGDGGGDCT--AAAAEDAESGFVDPGRVATEFGPSCKACR 300
           AAALQAQLQQAIM GAGI   G+GGGDCT  AAAAEDA S +VDP R+  EFGPSCK C 
Sbjct: 241 AAALQAQLQQAIMRGAGISSGGEGGGDCTSAAAAAEDAGSCYVDPERIGAEFGPSCKVCW 300

Query: 301 KRVASVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           KRVASVVLLPCRHLCVCTDCD VVRTCPLC A+RNSSVEVYLS
Sbjct: 301 KRVASVVLLPCRHLCVCTDCDQVVRTCPLCPAVRNSSVEVYLS 336

BLAST of Spg021172 vs. TAIR 10
Match: AT1G32740.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.9e-59
Identity = 163/343 (47.52%), Postives = 213/343 (62.10%), Query Frame = 0

Query: 1   MAVQAQ-YPSNVLLLN-RNIQDSDH-DYSLQPQPGGGGGVLQQSHLLFNNGGGNHLSNLR 60
           MAVQAQ + SN+L LN RN ++ +H +++LQ Q    G  L Q+++LFNNG     SN R
Sbjct: 1   MAVQAQHHSSNLLFLNKRNGKEKEHSNFTLQSQ--AAGDFLDQTNMLFNNGS----SNQR 60

Query: 61  KRGRDVSVSVSVSAPLINPLCFQ-PQIIDVSQLHNHHH-PSNVVSTGLRLSSG-DQPNSL 120
           KR R+ +     +  L+     Q PQ+ID+S LHN++H PSN+V TGLRL SG DQ   +
Sbjct: 61  KRRRETN-----NHQLLPMQSHQFPQVIDLSLLHNYNHPPSNMVHTGLRLFSGEDQAQKI 120

Query: 121 HHHPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQ 180
            H                        D F++ I +  +E+D+FL AQ EELRRTLAEKR+
Sbjct: 121 SH---------------------LSEDVFAAHINRQSEELDEFLHAQAEELRRTLAEKRK 180

Query: 181 RHYRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAA 240
            HY+ LLG  EE+  R+LREKEVE+E+ATRRH EL AR ++L  E Q WQ +A+A E AA
Sbjct: 181 MHYKALLGAVEESLVRKLREKEVEIERATRRHNELVARDSQLRAEVQVWQERAKAHEDAA 240

Query: 241 AALQAQLQQAIMSGAG------IGGSGDGGGDCTA-AAAEDAESGFVDPGRVATEFGPSC 300
           A+LQ+QLQQA+   AG         + + G  CT  +  +DAES +VDP RV     P+C
Sbjct: 241 ASLQSQLQQAVNQCAGGCVSAQDSRAAEEGLLCTTISGVDDAESVYVDPERVKR---PNC 300

Query: 301 KACRKRVASVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVE 331
           KACR+R A+VV+LPCRHL +C  CD     CPLC  LRNSSVE
Sbjct: 301 KACREREATVVVLPCRHLSICPGCDRTALACPLCLTLRNSSVE 308

BLAST of Spg021172 vs. TAIR 10
Match: AT5G47050.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 195.7 bits (496), Expect = 6.1e-50
Identity = 147/347 (42.36%), Postives = 192/347 (55.33%), Query Frame = 0

Query: 1   MAVQA-QYPSNVLLLNRNIQDSDHDYSLQPQPGGGGGVLQQSHLLFNNGGGNHLSNLRKR 60
           MAVQA  +PS +  LN   + +D  +              Q H +  N G     + RKR
Sbjct: 1   MAVQAHHHPSTLFFLNNGQEGNDQPHK------------SQFHSI--NAG----VDTRKR 60

Query: 61  GRDVSVSVSV---SAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHH 120
            R+VS  + +   +AP+  P    PQ+I            NVVSTGLRLS          
Sbjct: 61  AREVSSVIDLDITAAPMNPPPQTPPQVI------GRRQNPNVVSTGLRLS---------- 120

Query: 121 HPPPPPPQQDDTNLVSLSSSVFFSDDFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRH 180
                  ++   N      S   + D + +IK   DE+++FLQ Q E+L+R LAE  +R+
Sbjct: 121 -------REQSQNQEQRFLSFPITGDVAGEIKSQTDELNRFLQIQGEQLKRMLAENSERN 180

Query: 181 YRELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAA 240
           YRELL   EE+  RRLREKE E+EKATRRH ELEARA ++  EA+AWQ +A A EA A +
Sbjct: 181 YRELLRTTEESVRRRLREKEAEIEKATRRHVELEARATQIETEARAWQMRAAAREAEATS 240

Query: 241 LQAQLQQAIMSGAGIG---------GSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSC 300
           LQAQL QA++   G G         GS DG  +     AEDAES +VDP RV    GP C
Sbjct: 241 LQAQLHQAVVVAHGGGVITTVEPQSGSVDGVDE-----AEDAESAYVDPDRVEM-IGPGC 300

Query: 301 KACRKRVASVVLLPCRHLCVCTDCDHVVRTCPLCRALRNSSVEVYLS 335
           + CR+R A+V+ LPCRHL +CT+CD  VR CPLC + +NSSVEV+ S
Sbjct: 301 RICRRRSATVLALPCRHLVMCTECDGSVRICPLCLSTKNSSVEVFYS 300

BLAST of Spg021172 vs. TAIR 10
Match: AT4G17680.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.3e-48
Identity = 148/339 (43.66%), Postives = 190/339 (56.05%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNIQDSDHDYSLQPQPGGGGGVLQQSHLLFNNGGGNHLSNLRKRG 60
           MA+QAQ+PS     N N Q++  D SLQPQ              F   G     + RKR 
Sbjct: 18  MAIQAQHPSRFFFNNSNGQEAS-DCSLQPQ--------DTPFTNFTKAG----VDSRKRS 77

Query: 61  RDVSVSVSVSAPLINPLCFQPQIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHHHPPP 120
           R+V  S  ++ P   P     Q+ID+++L       NVVSTGLRL      N        
Sbjct: 78  REVYSSALMNPPPPKP----SQVIDITEL--LQKTPNVVSTGLRLFHDQSQN-------- 137

Query: 121 PPPQQDDTNLVSLSSSVFFSD---DFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHY 180
              QQ            FFS    D + +IK+ RDE+D+F+Q Q EELRRTLA+ R+R Y
Sbjct: 138 ---QQQ-----------FFSSLPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRY 197

Query: 181 RELLGVAEETAARRLREKEVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAAL 240
            ELL  AEE   R+LR+KE E+EKATRRHAELEAR A +  EA+ WQ +A   EA  ++L
Sbjct: 198 VELLCAAEEIVGRKLRKKEAELEKATRRHAELEARVAHIVEEARNWQLRAATREAEVSSL 257

Query: 241 QAQLQQAIM----SGAGIGGSGDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKR 300
            A LQQAI     + A     G+ GGD  A  AEDAES +VDP R+    GPSC+ CR++
Sbjct: 258 HAHLQQAIANRLDTAAKQSTFGEDGGD--AEEAEDAESVYVDPERIEL-IGPSCRICRRK 312

Query: 301 VASVVLLPCRHLCVCTDCD-HVVRTCPLCRALRNSSVEV 332
            A+V+ LPC+HL +C  CD   VR CP+C A++ S VEV
Sbjct: 318 SATVMALPCQHLILCNGCDVGAVRVCPICLAVKTSGVEV 312

BLAST of Spg021172 vs. TAIR 10
Match: AT4G17680.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 169.5 bits (428), Expect = 4.7e-42
Identity = 122/258 (47.29%), Postives = 156/258 (60.47%), Query Frame = 0

Query: 82  QIIDVSQLHNHHHPSNVVSTGLRLSSGDQPNSLHHHPPPPPPQQDDTNLVSLSSSVFFSD 141
           Q+ID+++L       NVVSTGLRL      N           QQ            FFS 
Sbjct: 10  QVIDITEL--LQKTPNVVSTGLRLFHDQSQN-----------QQQ-----------FFSS 69

Query: 142 ---DFSSQIKQHRDEIDQFLQAQEEELRRTLAEKRQRHYRELLGVAEETAARRLREKEVE 201
              D + +IK+ RDE+D+F+Q Q EELRRTLA+ R+R Y ELL  AEE   R+LR+KE E
Sbjct: 70  LPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRYVELLCAAEEIVGRKLRKKEAE 129

Query: 202 VEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQLQQAIM----SGAGIGGS 261
           +EKATRRHAELEAR A +  EA+ WQ +A   EA  ++L A LQQAI     + A     
Sbjct: 130 LEKATRRHAELEARVAHIVEEARNWQLRAATREAEVSSLHAHLQQAIANRLDTAAKQSTF 189

Query: 262 GDGGGDCTAAAAEDAESGFVDPGRVATEFGPSCKACRKRVASVVLLPCRHLCVCTDCD-H 321
           G+ GGD  A  AEDAES +VDP R+    GPSC+ CR++ A+V+ LPC+HL +C  CD  
Sbjct: 190 GEDGGD--AEEAEDAESVYVDPERIEL-IGPSCRICRRKSATVMALPCQHLILCNGCDVG 240

Query: 322 VVRTCPLCRALRNSSVEV 332
            VR CP+C A++ S VEV
Sbjct: 250 AVRVCPICLAVKTSGVEV 240

BLAST of Spg021172 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 113.2 bits (282), Expect = 4.0e-25
Identity = 99/325 (30.46%), Postives = 165/325 (50.77%), Query Frame = 0

Query: 40  QSHLLFNNGGG------NHLSN--------LRKRGRDVSVSVSVSAPLINPLCFQPQIID 99
           Q HLL N GGG      N+ +N          KRGR+     S+S    N +  Q Q + 
Sbjct: 30  QLHLLGNMGGGCTVDPVNYFANDNLVPMIRPNKRGREAE---SIS----NNIQRQQQKLQ 89

Query: 100 VSQLHNHHHPS--------NVVSTGLRLSSGDQPNSLHHHPPPPPPQQDDTNLVSLSSSV 159
           +S  +N+++ S        N+VSTGLRLS  D  ++              +  +  +S +
Sbjct: 90  MSLNYNYNNTSVREEVPKENLVSTGLRLSYDDDEHN--------SSVTSASGSILAASPI 149

Query: 160 FFSDDFSSQIKQHR--DEIDQFLQAQEEELRRTLAEKRQRHYRELLGVAEETAARRLREK 219
           F S D S +I  HR  DE DQF++ Q  ++ + + + +QRH    L   E+  +++L+EK
Sbjct: 150 FQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIASFLTTLEKGVSKKLQEK 209

Query: 220 EVEVEKATRRHAELEARAARLTMEAQAWQAKARAEEAAAAALQAQLQQAIMSGAGIGGSG 279
           + E+    +++ EL  R  ++ MEAQ W  +A+  E+    L+A LQQA+     +  + 
Sbjct: 210 DHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKANLQQAMSHNNSVIAAA 269

Query: 280 DGGGDCTA-AAAEDAESGFVDP-----GRVATEFGPSCKACRKRVASVVLLPCRHLCVCT 335
           D G +    +  +DA S ++DP       +       CK C  +  SV+++PCRHL +C 
Sbjct: 270 DQGKEGFGDSEIDDAASSYIDPNNNNNNNMGIHQRMRCKMCNVKEVSVLIVPCRHLSLCK 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147249.11.4e-14986.01BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN55804.1 hypothet... [more]
XP_008448752.14.2e-14684.66PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo][more]
XP_023005462.12.6e-14383.14E3 ubiquitin-protein ligase BOI-like [Cucurbita maxima][more]
XP_023539664.12.4e-14180.52E3 ubiquitin-protein ligase BOI-like [Cucurbita pepo subsp. pepo][more]
XP_022965101.12.4e-14183.73BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FHE41.1e-1432.21BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
O818511.4e-1433.49E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Q9LDD12.6e-1329.17Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
F4IDI63.4e-1330.65Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
O088635.2e-0645.83Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0L4346.8e-15086.01RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G016980 P... [more]
A0A1S3BL242.1e-14684.66probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1KT701.2e-14383.14E3 ubiquitin-protein ligase BOI-like OS=Cucurbita maxima OX=3661 GN=LOC111498441... [more]
A0A6J1HKS91.2e-14183.73BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita maxima OX... [more]
A0A6J1GEA78.3e-14081.63E3 ubiquitin-protein ligase BOI-like isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G32740.11.9e-5947.52SBP (S-ribonuclease binding protein) family protein [more]
AT5G47050.16.1e-5042.36SBP (S-ribonuclease binding protein) family protein [more]
AT4G17680.14.3e-4843.66SBP (S-ribonuclease binding protein) family protein [more]
AT4G17680.24.7e-4247.29SBP (S-ribonuclease binding protein) family protein [more]
AT1G10650.14.0e-2530.46SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 185..240
NoneNo IPR availableCOILSCoilCoilcoord: 147..175
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 286..323
e-value: 4.6E-8
score: 32.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 95..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 95..129
NoneNo IPR availablePANTHERPTHR42647:SF30BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1-LIKEcoord: 1..333
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 1..334
e-value: 2.6E-90
score: 301.4
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 1..333
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 280..334
e-value: 3.5E-11
score: 44.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 287..322
score: 10.246822

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg021172.1Spg021172.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity