Spg021136 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021136
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Protease inhibitor protein
Location	scaffold6: 48138827 .. 48139342 (-)
RNA-Seq Expression	Spg021136
Synteny	Spg021136

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGAATGTCCTCCAGGTATGTTCATTTCATATCGCTTAATCACTTGTGAATATGTTTAATTTGATTGTAAGTTGTGTATATTAGCAAATTTAATATGAATTACGTGTTTCTATATAGGAAAAAGCCAGTGGCCGGAGCTTGTTTTTGTAGAATATGATGTTGCTGCTGCGATCATTGAAAATGAGAATTCCAATGTCAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTCGATTGCACACGAGTTTGGGTTGATTGCAATATTAAAGATTATGTTGTTGAAATTCCTAAGGTTGGTTAAAGATTGTCCATGGCTATGTATGTATAAGTTATATGTATGTATATATGCATACATATAATGCATTTCAAATTACTAATAAGGTAGTATCTATTAACCTACCACTTCAATTTCAATTGAATTGTTCCAACCTGTCCCATTATTATCAAGCATTTAATTTTAGTTCAATTGAATTAAATTGTATCTAGAAAAAATTTAAAGATA

mRNA sequence

ATGTCTGAATGTCCTCCAGGAAAAAGCCAGTGGCCGGAGCTTGTTTTTGTAGAATATGATGTTGCTGCTGCGATCATTGAAAATGAGAATTCCAATGTCAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTCGATTGCACACGAGTTTGGGTTGATTGCAATATTAAAGATTATGTTGTTGAAATTCCTAAGGTTGGTTAA

Coding sequence (CDS)

ATGTCTGAATGTCCTCCAGGAAAAAGCCAGTGGCCGGAGCTTGTTTTTGTAGAATATGATGTTGCTGCTGCGATCATTGAAAATGAGAATTCCAATGTCAAAGCACACAAGATTCTAGCTGGGAGTCCGAGAATTTTGAACTTCGATTGCACACGAGTTTGGGTTGATTGCAATATTAAAGATTATGTTGTTGAAATTCCTAAGGTTGGTTAA

Protein sequence

MSECPPGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVEIPKVG

Homology

[Close]

BLAST of Spg021136 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 98.6 bits (244), Expect = 2.3e-17
Identity = 44/68 (64.71%), Postives = 56/68 (82.35%), Query Frame = 0


Query: 3  ECPPGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDY 62
          E  P K++WPELVFV+Y +AAAIIE EN +V+A KIL+G+PRILNFD  RVWVD N+++ 
Sbjct: 4  ESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEV 63

Query: 63 VVEIPKVG 71
          VV+ PKVG
Sbjct: 64 VVKKPKVG 71

[Close]

BLAST of Spg021136 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-16
Identity = 41/64 (64.06%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVE 65
          P K+QWPELVF++Y +AAAIIE EN +V+A KIL+G+PRILNFD  RVW D N++D +V+
Sbjct: 8  PRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVK 67

Query: 66 IPKV 70
          +PKV
Sbjct: 68 MPKV 71

[Close]

BLAST of Spg021136 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.1 bits (235), Expect = 2.5e-16
Identity = 43/63 (68.25%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 8  KSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVEIP 67
          K++WPELVFVE+  AA IIE EN +VKA KILA SPRI NFD +RVWVDCN++D VV++P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 68 KVG 71
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of Spg021136 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 91.7 bits (226), Expect = 2.8e-15
Identity = 43/65 (66.15%), Postives = 50/65 (76.92%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVE 65
          P K+QWPELVFV+Y  AAAIIE EN +VKA KIL+G  RI NFD  RVW D N++D VV+
Sbjct: 8  PRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVEDVVVK 67

Query: 66 IPKVG 71
           PKVG
Sbjct: 68 TPKVG 72

[Close]

BLAST of Spg021136 vs. NCBI nr
Match: KAE8650339.1 (hypothetical protein Csa_009729 [Cucumis sativus])

HSP 1 Score: 87.0 bits (214), Expect = 6.8e-14
Identity = 42/62 (67.74%), Postives = 48/62 (77.42%), Query Frame = 0


Query: 8  KSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVEIP 67
          KS WPELV V++  AA IIE EN NVKA KIL GSP ILN+D TRVWV CN +D VV+IP
Sbjct: 9  KSIWPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNTEDKVVDIP 68

Query: 68 KV 70
          +V
Sbjct: 69 RV 70

[Close]

BLAST of Spg021136 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.3e-09
Identity = 33/69 (47.83%), Postives = 41/69 (59.42%), Query Frame = 0


Query: 2  SECPPGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKD 61
          S C PGKS WP LV V   VA AIIE +N NVKA  +  G+P   +F C RV +  N + 
Sbjct: 1  SSC-PGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRG 60

Query: 62 YVVEIPKVG 71
           VV  P++G
Sbjct: 61 LVVSPPRIG 68

[Close]

BLAST of Spg021136 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.0e-08
Identity = 32/65 (49.23%), Postives = 40/65 (61.54%), Query Frame = 0


Query: 7   GKSQWPELVFVEYDVAAAIIENENSNV-KAHKILAGSPRILNFDCTRVWVDCNIKDYVVE 66
           GK +WPEL+ V   +A  IIE ENS +   H +L GSP  L+  C RV +  NI  YVV+
Sbjct: 43  GKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVD 102

Query: 67  IPKVG 71
           IP VG
Sbjct: 103 IPVVG 107

[Close]

BLAST of Spg021136 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.0e-08
Identity = 31/71 (43.66%), Postives = 42/71 (59.15%), Query Frame = 0


Query: 2  SECPPGKSQ-WPELVFVEYDVAAAIIENENSNV-KAHKILAGSPRILNFDCTRVWVDCNI 61
          S CP  K + WPEL+ V    A  II+ ENS +     +L GSP   +F C RV +  N+
Sbjct: 24 SSCPGNKKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNV 83

Query: 62 KDYVVEIPKVG 71
           D+VV+IP+VG
Sbjct: 84 LDFVVQIPRVG 94

[Close]

BLAST of Spg021136 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.4e-08
Identity = 28/64 (43.75%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVE 65
          PGK+ WPELV    ++AAA +E EN NV A  +  GS    +F C RVWV  N    V  
Sbjct: 5  PGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTS 64

Query: 66 IPKV 70
          +P +
Sbjct: 65 VPHI 68

[Close]

BLAST of Spg021136 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 4.5e-08
Identity = 33/71 (46.48%), Postives = 45/71 (63.38%), Query Frame = 0


Query: 2  SECPPGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSP--RILNFDCTRVWVDCNI 61
          S+CP  K+ WPELV    D+AA II+ EN+NVKA  +  G P  + LNF+  RV+VD N 
Sbjct: 3  SQCPV-KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDEN- 62

Query: 62 KDYVVEIPKVG 71
             V ++P +G
Sbjct: 63 -RVVTQVPAIG 70

[Close]

BLAST of Spg021136 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-17
Identity = 44/68 (64.71%), Postives = 56/68 (82.35%), Query Frame = 0


Query: 3  ECPPGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDY 62
          E  P K++WPELVFV+Y +AAAIIE EN +V+A KIL+G+PRILNFD  RVWVD N+++ 
Sbjct: 4  ESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEV 63

Query: 63 VVEIPKVG 71
          VV+ PKVG
Sbjct: 64 VVKKPKVG 71

[Close]

BLAST of Spg021136 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 7.1e-17
Identity = 41/64 (64.06%), Postives = 54/64 (84.38%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVE 65
          P K+QWPELVF++Y +AAAIIE EN +V+A KIL+G+PRILNFD  RVW D N++D +V+
Sbjct: 8  PRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVK 67

Query: 66 IPKV 70
          +PKV
Sbjct: 68 MPKV 71

[Close]

BLAST of Spg021136 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-16
Identity = 43/63 (68.25%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 8  KSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVEIP 67
          K++WPELVFVE+  AA IIE EN +VKA KILA SPRI NFD +RVWVDCN++D VV++P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 68 KVG 71
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of Spg021136 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-15
Identity = 43/65 (66.15%), Postives = 50/65 (76.92%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVE 65
          P K+QWPELVFV+Y  AAAIIE EN +VKA KIL+G  RI NFD  RVW D N++D VV+
Sbjct: 8  PRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVEDVVVK 67

Query: 66 IPKVG 71
           PKVG
Sbjct: 68 TPKVG 72

[Close]

BLAST of Spg021136 vs. ExPASy TrEMBL
Match: A0A0A0L785 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.0e-15
Identity = 41/63 (65.08%), Postives = 50/63 (79.37%), Query Frame = 0


Query: 8  KSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVEIP 67
          K++WPELVFV +  AA IIE EN +VKA KIL  SPRI NFD +RVWVDCNI++ VV++P
Sbjct: 9  KTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEERVVKVP 68

Query: 68 KVG 71
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of Spg021136 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 57.4 bits (137), Expect = 5.4e-09
Identity = 32/69 (46.38%), Postives = 38/69 (55.07%), Query Frame = 0


Query: 2  SECPPGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKD 61
          +EC P K+ WPEL     D AA +IE EN  V A  IL GSP   +F C RV V  +   
Sbjct: 3  TEC-PRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNR 62

Query: 62 YVVEIPKVG 71
           VV+ PK G
Sbjct: 63 IVVKTPKSG 70

[Close]

BLAST of Spg021136 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 53.5 bits (127), Expect = 7.8e-08
Identity = 29/65 (44.62%), Postives = 39/65 (60.00%), Query Frame = 0


Query: 6  PGKSQWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDYVVE 65
          PGKS WPEL+  + + A  +IE EN  +KA  IL G+     F C+RV+V  N    VV+
Sbjct: 35 PGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQ 94

Query: 66 IPKVG 71
          IP +G
Sbjct: 95 IPIIG 99

[Close]

BLAST of Spg021136 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 49.7 bits (117), Expect = 1.1e-06
Identity = 28/68 (41.18%), Postives = 34/68 (50.00%), Query Frame = 0


Query: 4  CPPGKS-QWPELVFVEYDVAAAIIENENSNVKAHKILAGSPRILNFDCTRVWVDCNIKDY 63
          C  GK  +WPELV     +A   IE EN NV A  +  G  RI NF C RV+V       
Sbjct: 18 CAIGKKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQ 77

Query: 64 VVEIPKVG 71
          V + P +G
Sbjct: 78 VADAPMIG 85

The following BLAST results are available for this feature:

BLAST of Spg021136 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN56897.1	2.3e-17	64.71	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049288.1	1.5e-16	64.06	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049286.1	2.5e-16	68.25	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KAA0049287.1	2.8e-15	66.15	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]
KAE8650339.1	6.8e-14	67.74	hypothetical protein Csa_009729 [Cucumis sativus]	[more]

BLAST of Spg021136 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	5.3e-09	47.83	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q00783	2.0e-08	49.23	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
Q02214	2.0e-08	43.66	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P82381	3.4e-08	43.75	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
Q6XNP7	4.5e-08	46.48	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]

BLAST of Spg021136 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8E6	1.1e-17	64.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A5A7U228	7.1e-17	64.06	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4U0	1.2e-16	68.25	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	1.3e-15	66.15	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L785	3.0e-15	65.08	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1	[more]

BLAST of Spg021136 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	5.4e-09	46.38	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	7.8e-08	44.62	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	1.1e-06	41.18	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..69 e-value: 6.5E-20 score: 73.2
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 3..70
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 8..70 e-value: 6.6E-17 score: 61.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 3..70
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 5..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021136.1	Spg021136.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity