Spg021099 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg021099
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Location	scaffold9: 1519572 .. 1520102 (-)
RNA-Seq Expression	Spg021099
Synteny	Spg021099

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGTTTTGAATCTAGAACTTGGATGGGCAGTGCGTGACTCCTCCGGGTCTTTGGTTATAGCCGGCTGCAAGAGGGTGGAGAGGAATTGGAGCATTCTGACGGCAGAAGCTTCTGCTATGAGAGAAGGCTTGTCCAACTTTCTGAAATCTAGCACATGCAACTCCCTCCCGCTCATCGTGGAGTCTGATTCGAGCGCTTTAGTGAAGATTCTGCAAGGGCATGAAGTCGATCTCTCTGAGGCAAAGAACATTGTGGACGATATCGAGGATTTGGCTAGAGAGGTGACCGAAATTTGTTTCGAGTGGTGTCCGAGGAGCTGCAATCAAGCAGCGCATACTCTCGCTCAAAAAGTGGGGAGCGTTGGTTCGATGTTCTTCTCATCGAATGATTTTTGTATGAACTCTGTAAATGATTTTGTGTGTTTTTCTCCTATGATGAAGGAGAATGGTTTTGGGCAAACGGTTTTACCTGAGTGGTTTTCCTCTTTATTGCATGAGGAAGTTAGTTGTATTGGTGTTGGCAGTTGA

mRNA sequence

ATGAGAGTTTTGAATCTAGAACTTGGATGGGCAGTGCGTGACTCCTCCGGGTCTTTGGTTATAGCCGGCTGCAAGAGGGTGGAGAGGAATTGGAGCATTCTGACGGCAGAAGCTTCTGCTATGAGAGAAGGCTTGTCCAACTTTCTGAAATCTAGCACATGCAACTCCCTCCCGCTCATCGTGGAGTCTGATTCGAGCGCTTTAGTGAAGATTCTGCAAGGGCATGAAGTCGATCTCTCTGAGGCAAAGAACATTGTGGACGATATCGAGGATTTGGCTAGAGAGGTGACCGAAATTTGTTTCGAGTGGTGTCCGAGGAGCTGCAATCAAGCAGCGCATACTCTCGCTCAAAAAGTGGGGAGCGTTGGTTCGATGTTCTTCTCATCGAATGATTTTTGTATGAACTCTGTAAATGATTTTGTGTGTTTTTCTCCTATGATGAAGGAGAATGGTTTTGGGCAAACGGTTTTACCTGAGTGGTTTTCCTCTTTATTGCATGAGGAAGTTAGTTGTATTGGTGTTGGCAGTTGA

Coding sequence (CDS)

ATGAGAGTTTTGAATCTAGAACTTGGATGGGCAGTGCGTGACTCCTCCGGGTCTTTGGTTATAGCCGGCTGCAAGAGGGTGGAGAGGAATTGGAGCATTCTGACGGCAGAAGCTTCTGCTATGAGAGAAGGCTTGTCCAACTTTCTGAAATCTAGCACATGCAACTCCCTCCCGCTCATCGTGGAGTCTGATTCGAGCGCTTTAGTGAAGATTCTGCAAGGGCATGAAGTCGATCTCTCTGAGGCAAAGAACATTGTGGACGATATCGAGGATTTGGCTAGAGAGGTGACCGAAATTTGTTTCGAGTGGTGTCCGAGGAGCTGCAATCAAGCAGCGCATACTCTCGCTCAAAAAGTGGGGAGCGTTGGTTCGATGTTCTTCTCATCGAATGATTTTTGTATGAACTCTGTAAATGATTTTGTGTGTTTTTCTCCTATGATGAAGGAGAATGGTTTTGGGCAAACGGTTTTACCTGAGTGGTTTTCCTCTTTATTGCATGAGGAAGTTAGTTGTATTGGTGTTGGCAGTTGA

Protein sequence

MRVLNLELGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSALVKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSMFFSSNDFCMNSVNDFVCFSPMMKENGFGQTVLPEWFSSLLHEEVSCIGVGS

Homology

[Close]

BLAST of Spg021099 vs. NCBI nr
Match: CAB4279811.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 74.7 bits (182), Expect = 8.8e-10
Identity = 48/119 (40.34%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 8    LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
            +GW VRDS G +V AGCK   R  S L  EA A+RE L   ++S       L VESDS  
Sbjct: 1084 VGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACVQSGLSK---LEVESDSLQ 1143

Query: 68   LVKILQGH-EVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSM 126
            L+++L G   +D++  + IV DI+ L  +     F + PR+CN+AAH +   V  VG +
Sbjct: 1144 LIRMLGGEWNIDIA-VETIVFDIKQLVCQFQHCVFLYTPRNCNKAAHKITAFVSRVGGV 1198

[Close]

BLAST of Spg021099 vs. NCBI nr
Match: ONI22498.1 (hypothetical protein PRUPE_2G133200 [Prunus persica])

HSP 1 Score: 72.8 bits (177), Expect = 3.4e-09
Identity = 42/119 (35.29%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 9   GWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSAL 68
           GW +RD  G ++ AG KR  R  S L AE  A+R  +    +    +   +IVESDS + 
Sbjct: 78  GWVIRDFVGWMLQAGGKRDRRYGSALIAEVDAIRAAVVACQQGGFSH---IIVESDSLSA 137

Query: 69  VKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSMFF 128
           +++++G  V  +E + ++ DI  + RE+ E+ F + PRSCN AAH +A      G +F+
Sbjct: 138 IQMVKGDRVVDAEVEGLLFDIHAVTRELQEVTFTYAPRSCNMAAHEVAAFACRNGGLFW 193

[Close]

BLAST of Spg021099 vs. NCBI nr
Match: CAB4273082.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 69.3 bits (168), Expect = 3.7e-08
Identity = 46/119 (38.66%), Postives = 66/119 (55.46%), Query Frame = 0


Query: 8    LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
            +GW VRDS G +V AG K   R  S L  EA A+RE L   ++S       L VESDS  
Sbjct: 1172 VGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIREALVACVQSGLSK---LEVESDSLQ 1231

Query: 68   LVKILQGH-EVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSM 126
            +++++ G   +D++    IV DI+ L  +     F + PR+CN+AAH +A  V  VG +
Sbjct: 1232 VIRMIGGEWNMDIA-VDTIVFDIKQLVGQFQHCVFLYTPRNCNKAAHKIAAFVSRVGGV 1286

[Close]

BLAST of Spg021099 vs. NCBI nr
Match: CAB4303756.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 68.6 bits (166), Expect = 6.3e-08
Identity = 42/116 (36.21%), Postives = 62/116 (53.45%), Query Frame = 0


Query: 8   LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
           +GW +RDS G L+ AG +      S +  E  A+R  LS   + +  +   +IVESDS  
Sbjct: 821 VGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAIRHALS---ECANFHLTDIIVESDSQK 880

Query: 68  LVKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVG 124
            + +L G     S+ + IV DI  L   ++ + F + PRSCNQAAH++A  V   G
Sbjct: 881 GILMLNGRATVDSDLEGIVFDIRQLVASLSRVSFVFAPRSCNQAAHSVAGFVSKQG 933

[Close]

BLAST of Spg021099 vs. NCBI nr
Match: CAB4274760.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-07
Identity = 40/109 (36.70%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 8    LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
            +GW +RDS G L+ AG +      S +  E  A+R  LS   + +  +   +IVESDS  
Sbjct: 1152 VGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAIRHALS---ECANFHLTDIIVESDSQK 1211

Query: 68   LVKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLA 117
             + +L G     S+ + IV DI  L   ++ + F + PRSCNQAAH++A
Sbjct: 1212 GILMLNGRATVDSDLEGIVFDIRQLVASLSRVSFVFAPRSCNQAAHSVA 1257

[Close]

BLAST of Spg021099 vs. ExPASy TrEMBL
Match: A0A6J5UV01 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS32434 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 4.3e-10
Identity = 48/119 (40.34%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 8    LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
            +GW VRDS G +V AGCK   R  S L  EA A+RE L   ++S       L VESDS  
Sbjct: 1084 VGWVVRDSYGWMVQAGCKGDMRGGSALAMEAKAIREALLACVQSGLSK---LEVESDSLQ 1143

Query: 68   LVKILQGH-EVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSM 126
            L+++L G   +D++  + IV DI+ L  +     F + PR+CN+AAH +   V  VG +
Sbjct: 1144 LIRMLGGEWNIDIA-VETIVFDIKQLVCQFQHCVFLYTPRNCNKAAHKITAFVSRVGGV 1198

[Close]

BLAST of Spg021099 vs. ExPASy TrEMBL
Match: A0A251QFD7 (RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G133200 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.6e-09
Identity = 42/119 (35.29%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 9   GWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSAL 68
           GW +RD  G ++ AG KR  R  S L AE  A+R  +    +    +   +IVESDS + 
Sbjct: 78  GWVIRDFVGWMLQAGGKRDRRYGSALIAEVDAIRAAVVACQQGGFSH---IIVESDSLSA 137

Query: 69  VKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSMFF 128
           +++++G  V  +E + ++ DI  + RE+ E+ F + PRSCN AAH +A      G +F+
Sbjct: 138 IQMVKGDRVVDAEVEGLLFDIHAVTRELQEVTFTYAPRSCNMAAHEVAAFACRNGGLFW 193

[Close]

BLAST of Spg021099 vs. ExPASy TrEMBL
Match: M5X3Z4 (RNase H domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa020941mg PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 4.7e-09
Identity = 40/108 (37.04%), Postives = 63/108 (58.33%), Query Frame = 0


Query: 9   GWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSAL 68
           GW +RD  G ++ AG KR  R  S L AE  A+R  +    +    +   +IVESDS + 
Sbjct: 73  GWVIRDFVGWMLQAGGKRDRRYGSALIAEVDAIRAAVVACQQGGFSH---IIVESDSLSA 132

Query: 69  VKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLA 117
           +++++G  V  +E + ++ DI  + RE+ E+ F + PRSCN AAH +A
Sbjct: 133 IQMVKGDRVVDAEVEGLLFDIHAVTRELQEVTFTYAPRSCNMAAHEVA 177

[Close]

BLAST of Spg021099 vs. ExPASy TrEMBL
Match: A0A6J5UAY2 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS20161 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.8e-08
Identity = 46/119 (38.66%), Postives = 66/119 (55.46%), Query Frame = 0


Query: 8    LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
            +GW VRDS G +V AG K   R  S L  EA A+RE L   ++S       L VESDS  
Sbjct: 1172 VGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIREALVACVQSGLSK---LEVESDSLQ 1231

Query: 68   LVKILQGH-EVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSM 126
            +++++ G   +D++    IV DI+ L  +     F + PR+CN+AAH +A  V  VG +
Sbjct: 1232 VIRMIGGEWNMDIA-VDTIVFDIKQLVGQFQHCVFLYTPRNCNKAAHKIAAFVSRVGGV 1286

[Close]

BLAST of Spg021099 vs. ExPASy TrEMBL
Match: A0A6J5WPU6 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS20372 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.1e-08
Identity = 42/116 (36.21%), Postives = 62/116 (53.45%), Query Frame = 0


Query: 8   LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
           +GW +RDS G L+ AG +      S +  E  A+R  LS   + +  +   +IVESDS  
Sbjct: 821 VGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAIRHALS---ECANFHLTDIIVESDSQK 880

Query: 68  LVKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVG 124
            + +L G     S+ + IV DI  L   ++ + F + PRSCNQAAH++A  V   G
Sbjct: 881 GILMLNGRATVDSDLEGIVFDIRQLVASLSRVSFVFAPRSCNQAAHSVAGFVSKQG 933

[Close]

BLAST of Spg021099 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 3.0e-08
Identity = 38/124 (30.65%), Postives = 65/124 (52.42%), Query Frame = 0


Query: 8   LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
           +GW +R+ SG ++  G + + R  ++L AE  A+R  +   L  S  N   +I ESD+ A
Sbjct: 162 IGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAV---LTMSRFNYKRIIFESDAQA 221

Query: 68  LVKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQKVGSVGSMFF 127
           LV +L   +      +  ++DI+ L     E+ FE+ PR  N+ A  +A++     S+ F
Sbjct: 222 LVNLLNSDDF-WPTLQPALEDIQQLLHHFEEVKFEFTPRGGNKVADRIARE-----SISF 276

Query: 128 SSND 132
           S+ D
Sbjct: 282 SNYD 276

[Close]

BLAST of Spg021099 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 4.4e-07
Identity = 34/111 (30.63%), Postives = 56/111 (50.45%), Query Frame = 0


Query: 8   LGWAVRDSSGSLVIAGCKRVERNWSILTAEASAMREGLSNFLKSSTCNSLPLIVESDSSA 67
           +GW +R+  G +   G + + +  S+L AE  AMR  +   L  S      +I ESDS  
Sbjct: 445 IGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAV---LSLSRFQYNYVIFESDSQV 504

Query: 68  LVKILQGHEVDLSEAKNIVDDIEDLAREVTEICFEWCPRSCNQAAHTLAQK 119
           L++IL   E+     K  + D++ L  + TE+ F + PR  N  A  +A++
Sbjct: 505 LIEILNNDEI-WPSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLAERVARE 551

The following BLAST results are available for this feature:

BLAST of Spg021099 vs. NCBI nr


Match Name	E-value	Identity	Description
CAB4279811.1	8.8e-10	40.34	unnamed protein product [Prunus armeniaca]	[more]
ONI22498.1	3.4e-09	35.29	hypothetical protein PRUPE_2G133200 [Prunus persica]	[more]
CAB4273082.1	3.7e-08	38.66	unnamed protein product [Prunus armeniaca]	[more]
CAB4303756.1	6.3e-08	36.21	unnamed protein product [Prunus armeniaca]	[more]
CAB4274760.1	1.8e-07	36.70	unnamed protein product [Prunus armeniaca]	[more]

BLAST of Spg021099 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg021099 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J5UV01	4.3e-10	40.34	Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=...	[more]
A0A251QFD7	1.6e-09	35.29	RNase H domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G133200 PE...	[more]
M5X3Z4	4.7e-09	37.04	RNase H domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_...	[more]
A0A6J5UAY2	1.8e-08	38.66	Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=...	[more]
A0A6J5WPU6	3.1e-08	36.21	Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=...	[more]

BLAST of Spg021099 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	3.0e-08	30.65	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	4.4e-07	30.63	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 72..92
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 7..128
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 7..128
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 8..118 e-value: 6.8E-23 score: 80.8
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 5..122 e-value: 1.9E-13 score: 52.5
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 9..116 e-value: 1.836E-17 score: 72.3468
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 9..119

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg021099.1	Spg021099.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity