Spg020950 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg020950
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionprotein NRT1/ PTR FAMILY 5.10-like
Locationscaffold9: 1234332 .. 1237170 (+)
RNA-Seq ExpressionSpg020950
SyntenySpg020950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAGGTTCAGTCCATGTGCAAGCCAAGTGTAGAAATCAAATTGTTGTGATAGCAGGCAGAGTTGTTAGTTTTGTTTTTATTATCCGTTGGTGTTCTTAGTCTAGATAAGGAGTTTTTCATCTCTATTTTTACCAGTATATATGTAGCTGCATCCAAGTAAATTGATGGAGCTCAATTATTGAATGGGATAGAGATTTTCTCCGTCCATTTACTCTGTTCTTCTCTTTTATTCGTTTCGAGGGACTCTGTAGATCTCACCGAGGCGGCGCTGTTCGTCTGATTTGGGTAGATTGGCAAATGCGGTACTGTTTAGACATATTAACAAAGAGTGCGCCAAAAGCATCCATGGAAGGCGTTGATGGCATTTGGAAATGGTTACTCTCTTGCACGCGGAGAGATGGATGGAGTGGTAGTGGTTGGCTAGTGGTTTAATGTCTTCTTCTTTTGTTTTTATTGTCTTTTGTTGCCTTTCTGTTTCTCTATGATCAAAAAATTAAATAAGCTTGATTGCAAACGGAGCCAATAATTGGGAGATTGGGAGTAGAGGCGGCTCAGCCAAATGGAGGTTCCTCTGTTGGAGGAGACGGTGGAGGGCGCAGTCGACTACAGTGGCCGGCCAGTCCGCAGATCCAACTCCGGCGGCTGGAGATCCGCCTCACTCATCATAGGTAAATCTAAATATCATCAATCGCCCCATCTCTTTCTTCTGATTCGCTGTCCAATATTTGTATCCCTCTCGAATTGTGAGTAGGAGTGGAGGCTGCCGAGAGATTCGCCTACTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCATCAGTCCACGGCCACTGCAGCGGAGAACGTCAATGCCTGGTCCGGCGCCGCCTCCTTGCTACCTCTTCTCGGCGCCTTTCTCGCCGATTCCTTCCTTGGACGATACCGCACTATTGCTCTTTCCTCTGCCCTCTACATCCTGGTTCCTCCACTACTCTATTCTCCTTTAATTAGGGCTCTCCTAATTCGGGATTATTCTAATTAGGGCTCTACAAACTGGATAATTTCACAATATAGTTTTCTTTTGGAATTCAGGGGCTTGGGTTGTTGACTGTGTCTGCAACATTACCTTCTCCCAGCACTTCTGCCTGCCAACCGACGGAAAAATCCGTAGCATGTTCCCCCAGTCTTGTCCAACTAGTTTCATTTTTCTTCTCCTTATATCTGGTGGCATTTGCTCAAGGCGGACACAAGCCTTGCGTCCAGGCTTTCGGAGCCGACCAATTTGATGGACAACATCCAGAAGAGAACAAAGCTAAAAGCTCTTTCTTTAATTGGTGGTACTTTGGTCTCTCTCTGGCCACTCTGGCAACTGTTAATATCTTGAACTATGTGCAAGATAACCTTAGCTGGAGTCTTGGGTTTGGAATTTCTTGTACTGCTATGGTCTTTGCATTCCTAGTCTTCTTGCTTGGAACAAGGACGTACAGGTACAGTAACATAACAGATGAGAAAAACCCATTGGTGAGGATTGGACGGGTGTTCATCACGGCAGTAAGAAATTGGCATGTAAAATCTTCAGAAATTGCTCATGAAGAGGAAATCCGTGGCCTTTTACCTCACCATACCTCTCAACAATTAAGGTGATACTCATTACATGGATCTAAAACGCAACTTTTCTTTATAAACCATTTTAATTTGCTTCTATCATTATAATCCTTTTCAGCCTTTGGATCCTCTAATTTGGCAGGTTCCTGGACAAAGCTTTAGTTGCACCTAATGGTTCAAAGGAAGACAGTCACGCATGTAGTGTCGGCGAAGTTGAGGAAGCAAAAGCAGTACTTAGGCTTGCTCCCATATGGGTTACATGCTTAGCATATGCTATTGTATTCTCTCAGTTTTCTACTTTCTTTACAAAACAAGGAGTCACAATGGACAGATCAATAGTTCCTGGATTTGAAATACCTGCTGCTTCCCTTCAATCCTTTATCGGTCTGTCCATTGTCATTTCCCTCCCGATATATGATCGCATACTCATACTGATTGCGAGAAATTTCACTGGAAAACCTTCTGGAATTACAATGCTACAGAGAATTGGATTTGGGATGTTAGTATCTGTCCTTTCCATGATCGTTGCTGCAGTGGTCGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCGAAGGCGACTGTTCCTCTGAGTATATGGTGGCTGGTTCCTCAATATGTACTGTTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGATCAGGTTCCAAGTGGATTAAGAAGCATCGGTCTGTCCCCGTACCTGAGTATTTTTGGCGTTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAGGTTAACCAGTGGGGATGGCAGAGAAAGCTGGTTTGACAATAACCTCAACAAAGCTCATCTTGATTACTTCTACTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTTCTATGTGTTGCAAGAACTTACATCTACAATAAGGGGAACACCATATGAATACAACCGTACTATGGGATTGGAAATGCATTATTATGTATTGATATTGAACCATTTCAAACTTCATTTCATTATGAGGAATTCCAGTTTTAGAATCGTCCTCAGATTTTCAGGATGGTTGTTCACAAAGCTTTCCTACCATTTGCAGAAACTAACATCCACAGTAGGAGGAATACCACATGAATTATTATTTTTATTTTTTTTTTTTTTGAGTACATCACCTACATGTTAATTACCGCTGAGCTAAGCTCGCTTTGGGAAAATGAAATCTGAAAAATTTGGTA

mRNA sequence

ATGGAGGTTCCTCTGTTGGAGGAGACGGTGGAGGGCGCAGTCGACTACAGTGGCCGGCCAGTCCGCAGATCCAACTCCGGCGGCTGGAGATCCGCCTCACTCATCATAGTGGAGGCTGCCGAGAGATTCGCCTACTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCATCAGTCCACGGCCACTGCAGCGGAGAACGTCAATGCCTGGTCCGGCGCCGCCTCCTTGCTACCTCTTCTCGGCGCCTTTCTCGCCGATTCCTTCCTTGGACGATACCGCACTATTGCTCTTTCCTCTGCCCTCTACATCCTGGGGCTTGGGTTGTTGACTGTGTCTGCAACATTACCTTCTCCCAGCACTTCTGCCTGCCAACCGACGGAAAAATCCGTAGCATGTTCCCCCAGTCTTGTCCAACTAGTTTCATTTTTCTTCTCCTTATATCTGGTGGCATTTGCTCAAGGCGGACACAAGCCTTGCGTCCAGGCTTTCGGAGCCGACCAATTTGATGGACAACATCCAGAAGAGAACAAAGCTAAAAGCTCTTTCTTTAATTGGTGGTACTTTGGTCTCTCTCTGGCCACTCTGGCAACTGTTAATATCTTGAACTATGTGCAAGATAACCTTAGCTGGAGTCTTGGGTTTGGAATTTCTTGTACTGCTATGGTCTTTGCATTCCTAGTCTTCTTGCTTGGAACAAGGACGTACAGGTACAGTAACATAACAGATGAGAAAAACCCATTGGTGAGGATTGGACGGGTGTTCATCACGGCAGTAAGAAATTGGCATGTAAAATCTTCAGAAATTGCTCATGAAGAGGAAATCCGTGGCCTTTTACCTCACCATACCTCTCAACAATTAAGGTTCCTGGACAAAGCTTTAGTTGCACCTAATGGTTCAAAGGAAGACAGTCACGCATGTAGTGTCGGCGAAGTTGAGGAAGCAAAAGCAGTACTTAGGCTTGCTCCCATATGGGTTACATGCTTAGCATATGCTATTGTATTCTCTCAGTTTTCTACTTTCTTTACAAAACAAGGAGTCACAATGGACAGATCAATAGTTCCTGGATTTGAAATACCTGCTGCTTCCCTTCAATCCTTTATCGGTCTGTCCATTGTCATTTCCCTCCCGATATATGATCGCATACTCATACTGATTGCGAGAAATTTCACTGGAAAACCTTCTGGAATTACAATGCTACAGAGAATTGGATTTGGGATGTTAGTATCTGTCCTTTCCATGATCGTTGCTGCAGTGGTCGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCGAAGGCGACTGTTCCTCTGAGTATATGGTGGCTGGTTCCTCAATATGTACTGTTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGATCAGGTTCCAAGTGGATTAAGAAGCATCGGTCTGTCCCCGTACCTGAGTATTTTTGGCGTTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAGGTTAACCAGTGGGGATGGCAGAGAAAGCTGGTTTGACAATAACCTCAACAAAGCTCATCTTGATTACTTCTACTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTTCTATGTGTTGCAAGAACTTACATCTACAATAAGGGGAACACCATATGA

Coding sequence (CDS)

ATGGAGGTTCCTCTGTTGGAGGAGACGGTGGAGGGCGCAGTCGACTACAGTGGCCGGCCAGTCCGCAGATCCAACTCCGGCGGCTGGAGATCCGCCTCACTCATCATAGTGGAGGCTGCCGAGAGATTCGCCTACTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCATCAGTCCACGGCCACTGCAGCGGAGAACGTCAATGCCTGGTCCGGCGCCGCCTCCTTGCTACCTCTTCTCGGCGCCTTTCTCGCCGATTCCTTCCTTGGACGATACCGCACTATTGCTCTTTCCTCTGCCCTCTACATCCTGGGGCTTGGGTTGTTGACTGTGTCTGCAACATTACCTTCTCCCAGCACTTCTGCCTGCCAACCGACGGAAAAATCCGTAGCATGTTCCCCCAGTCTTGTCCAACTAGTTTCATTTTTCTTCTCCTTATATCTGGTGGCATTTGCTCAAGGCGGACACAAGCCTTGCGTCCAGGCTTTCGGAGCCGACCAATTTGATGGACAACATCCAGAAGAGAACAAAGCTAAAAGCTCTTTCTTTAATTGGTGGTACTTTGGTCTCTCTCTGGCCACTCTGGCAACTGTTAATATCTTGAACTATGTGCAAGATAACCTTAGCTGGAGTCTTGGGTTTGGAATTTCTTGTACTGCTATGGTCTTTGCATTCCTAGTCTTCTTGCTTGGAACAAGGACGTACAGGTACAGTAACATAACAGATGAGAAAAACCCATTGGTGAGGATTGGACGGGTGTTCATCACGGCAGTAAGAAATTGGCATGTAAAATCTTCAGAAATTGCTCATGAAGAGGAAATCCGTGGCCTTTTACCTCACCATACCTCTCAACAATTAAGGTTCCTGGACAAAGCTTTAGTTGCACCTAATGGTTCAAAGGAAGACAGTCACGCATGTAGTGTCGGCGAAGTTGAGGAAGCAAAAGCAGTACTTAGGCTTGCTCCCATATGGGTTACATGCTTAGCATATGCTATTGTATTCTCTCAGTTTTCTACTTTCTTTACAAAACAAGGAGTCACAATGGACAGATCAATAGTTCCTGGATTTGAAATACCTGCTGCTTCCCTTCAATCCTTTATCGGTCTGTCCATTGTCATTTCCCTCCCGATATATGATCGCATACTCATACTGATTGCGAGAAATTTCACTGGAAAACCTTCTGGAATTACAATGCTACAGAGAATTGGATTTGGGATGTTAGTATCTGTCCTTTCCATGATCGTTGCTGCAGTGGTCGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCGAAGGCGACTGTTCCTCTGAGTATATGGTGGCTGGTTCCTCAATATGTACTGTTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGATCAGGTTCCAAGTGGATTAAGAAGCATCGGTCTGTCCCCGTACCTGAGTATTTTTGGCGTTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAGGTTAACCAGTGGGGATGGCAGAGAAAGCTGGTTTGACAATAACCTCAACAAAGCTCATCTTGATTACTTCTACTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTTCTATGTGTTGCAAGAACTTACATCTACAATAAGGGGAACACCATATGA

Protein sequence

MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIVEAAERFAYYGVSSNLINFLTDQLHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVAPNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPGFEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIVAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSAIGLAAFLCVARTYIYNKGNTI
Homology
BLAST of Spg020950 vs. NCBI nr
Match: XP_023535812.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 963.0 bits (2488), Expect = 1.1e-276
Identity = 484/562 (86.12%), Postives = 521/562 (92.70%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LII VE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S+CQ TEKS+ CSP L Q++ FF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW + SSEIAHEEEIRGLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN SKE  H C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           FE+PAASLQS IGLSIV+SLPIYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SA+GLAAFLC ARTYIY KGNT
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNT 562

BLAST of Spg020950 vs. NCBI nr
Match: XP_023535811.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 962.2 bits (2486), Expect = 1.9e-276
Identity = 483/562 (85.94%), Postives = 519/562 (92.35%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LII VE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVN WSG ASLLPLLGAFLADSF+GRYRTIALSS LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S+CQ TEKS+ CSP L Q++ FF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW + SSEIAHEEEIRGLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN SKE  H C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           FE+PAASLQS IGLSIV+SLPIYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SA+GLAAFLC ARTYIY KGNT
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNT 562

BLAST of Spg020950 vs. NCBI nr
Match: XP_023535809.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535810.1 protein NRT1/ PTR FAMILY 5.10-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023535814.1 protein NRT1/ PTR FAMILY 5.10-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 959.5 bits (2479), Expect = 1.3e-275
Identity = 484/562 (86.12%), Postives = 520/562 (92.53%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LII VE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S+CQ TEKS+ CSP L Q++ FF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW V SSEIAHEEE RGLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEETRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN SKE  H C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLLPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           F++PAASLQSFIGLSIV+SLPIYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSSLSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SAIGLAAFLC ARTYIY+K NT
Sbjct: 541 SAIGLAAFLCFARTYIYSKKNT 562

BLAST of Spg020950 vs. NCBI nr
Match: XP_023535815.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 956.4 bits (2471), Expect = 1.1e-274
Identity = 481/562 (85.59%), Postives = 515/562 (91.64%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLL+ETVEGAVDY+GRPV RS  GGWRSAS II VE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLDETVEGAVDYNGRPVLRSKVGGWRSASFIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVN WSG ASLLPLLGAFLADSF+GRYRTIALSS LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S+CQ TEKS+ CSP L Q++ FF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW + SSEIAHEEEIRGLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN SKE  H C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           FE+PAASLQS IGLSIV+SLPIYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SA+GLAAFLC ARTYIY KGNT
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNT 562

BLAST of Spg020950 vs. NCBI nr
Match: XP_022973964.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima])

HSP 1 Score: 956.1 bits (2470), Expect = 1.4e-274
Identity = 483/562 (85.94%), Postives = 518/562 (92.17%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LII VE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SP  S CQ TEKS+ CSP L Q++ FF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW V SSEIAHEEEIRGLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN SKE  H CS+ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           F++PAASLQSFI LSIV+SL IYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF++NLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SAIGLAAFLC ARTYIY+KGNT
Sbjct: 541 SAIGLAAFLCFARTYIYSKGNT 562

BLAST of Spg020950 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 4.1e-205
Identity = 368/559 (65.83%), Postives = 446/559 (79.79%), Query Frame = 0

Query: 4   PLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQLHQ 63
           PLL  T    VDY  +P  +S+SGGWRSA  II VE AERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSP 123
           STA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +SALYI+GLG+LT+SA +PS 
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSD 132

Query: 124 STSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKA 183
               C+ +    +CSP   Q+++FF +LYLVA AQGGHKPCVQAFGADQFD + PEE KA
Sbjct: 133 ----CKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKA 192

Query: 184 KSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYS 243
           KSSFFNWWYFG+   TL T+ +LNY+QDNLSW+LGFGI C AMV A +V LLGT TYR+S
Sbjct: 193 KSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFS 252

Query: 244 NITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVAPNG 303
              ++++P VRIG V++ AV+NW V + ++A  EE  GL+   +SQQ  FL+KALVA NG
Sbjct: 253 IRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNG 312

Query: 304 SKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPGFEI 363
           S      CS+ E+EEAK+VLRLAPIW+TCL YA+VF+Q  TFFTKQG TM+RSI PG++I
Sbjct: 313 S------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKI 372

Query: 364 PAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIVAAV 423
             A+LQSFI LSIVI +PIYDR+LI IAR+FT KP GITMLQRIG G+ +S L+M+VAA+
Sbjct: 373 SPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAAL 432

Query: 424 VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 483
           VE+KRLKTA +YGLVD P ATVP+S+WWLVPQYVLFG+ DVF MVGLQEFFYDQVP+ LR
Sbjct: 433 VEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELR 492

Query: 484 SIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 543
           S+GL+ YLSIFG+GNFLSSF+IS IE+ TS  G+ SWF NNLN+AHLDYFYWLLA LS I
Sbjct: 493 SVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFI 552

Query: 544 GLAAFLCVARTYIYNKGNT 562
           GLA++L VA++Y+  + +T
Sbjct: 553 GLASYLYVAKSYVSKRLDT 556

BLAST of Spg020950 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 604.4 bits (1557), Expect = 1.3e-171
Identity = 309/560 (55.18%), Postives = 405/560 (72.32%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQL 61
           E+ L EE V  AVD+ G   RRSN+G WR+A  II VE AERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            +STA AA NVNAWSG A+LLP+LGAF+AD+FLGRYRTI +SS +Y+LGL  LT+SA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFL- 125

Query: 122 SPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
                   P    V  S S    V FFFSLYLVA  Q GHKPCVQAFGADQFD +  +E 
Sbjct: 126 -------IPNTTEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 182 KAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
             +SSFFNWWY  LS      + ++ Y+Q+  SW+ GFGI C  MV + ++F+ G R YR
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 242 YSNITDEK--NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALV 301
           YS    E+  NP  RIGRVF  A++N  + SS++   E    L  + + ++  F +KAL+
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALL 305

Query: 302 APNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVP 361
            PN S +  +A    +VE+A A++RL P+W T LAYAI ++Q+ TFFTKQGVTMDR+I+P
Sbjct: 306 VPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILP 365

Query: 362 GFEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMI 421
           G +IP ASLQ FIG+SIV+ +PIYDR+ + IAR  T +P GIT L+RIG G+++S ++M+
Sbjct: 366 GVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMV 425

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           +AA+VE KRL+TA+E+GL+D P+AT+P+SIWWL+PQY+L G+ADV+T+VG+QEFFY QVP
Sbjct: 426 IAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVP 485

Query: 482 SGLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRSIGL+ YLS  GVG+ LSS LIS I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAI 545

Query: 542 LSAIGLAAFLCVARTYIYNK 559
           +SA+G   FL ++++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 553

BLAST of Spg020950 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 604.0 bits (1556), Expect = 1.8e-171
Identity = 309/561 (55.08%), Postives = 403/561 (71.84%), Query Frame = 0

Query: 5   LLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQLHQS 64
           L EE V  AVD+ G   RRS +G WR+A  II VE AERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPS 124
           TA AA NVNAWSG +++LPLLGAF+AD+FLGRY TI ++S +Y+LGL  LT+SA L    
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFL---- 128

Query: 125 TSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAK 184
                P    V  SPS      FFFSLYLVA  Q GHKPCVQAFGADQFD ++P+EN  +
Sbjct: 129 ----IPNNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDR 188

Query: 185 SSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSN 244
           SSFFNWWY  +       + ++ Y+Q+N+SW+LGFGI C  MV + ++F+LG ++YR+S 
Sbjct: 189 SSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSK 248

Query: 245 I--TDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVAPN 304
               +E NP  RIGRVF  A +N  + SS++   E I       + ++L FL+KAL+ PN
Sbjct: 249 TRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPN 308

Query: 305 GSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPGFE 364
            S E   AC   +VE+A A++RL P+W+T LAYAI F+Q+ TFFTKQGVTM+R+I PG E
Sbjct: 309 DSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVE 368

Query: 365 IPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIVAA 424
           IP ASLQ  I +SIV+ +PIYDR+L+ I R+ T  P GIT L+RIG GM+++ L+M+VAA
Sbjct: 369 IPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAA 428

Query: 425 VVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 484
           +VE KRL+TA+EYGL+D PK T+P+SIWWL PQY+L G+ADV T+VG+QEFFY QVP+ L
Sbjct: 429 LVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTEL 488

Query: 485 RSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSA 544
           RS+GL+ YLS  GVG+ LSS LI  I+  T GD   SWF++NLN+AHLDYFYWLLA +SA
Sbjct: 489 RSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSA 548

Query: 545 IGLAAFLCVARTYIYNKGNTI 563
           +G   FL ++++YIY + + +
Sbjct: 549 VGFFTFLFISKSYIYRRVDVV 561

BLAST of Spg020950 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 9.9e-167
Identity = 304/560 (54.29%), Postives = 400/560 (71.43%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQL 61
           E  L+E++V  +VD+ G P  +S++GGWRSA  II VE  ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S +YILGLGLLT+S+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 122 SPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
               S  +   ++ +  P     + FF SLYLVA  QGGHKPCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 182 KAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
            ++ SFFNWW+  LS     ++ ++ YVQDN++W+LGFGI C  MV A  +FL G +TYR
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 242 Y--SNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALV 301
           Y   +   + N   RIGRVF+ A +N  +K   + H  ++          QL FL KAL+
Sbjct: 247 YPRGDREGKNNAFARIGRVFLVAFKNRKLK---LTHSGQLEVGSYKKCKGQLEFLAKALL 306

Query: 302 APNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVP 361
              G  E    CS  +VE+A A++RL PIW+T +   I ++Q++TFFTKQGVT+DR I+P
Sbjct: 307 PGEGGVE---PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILP 366

Query: 362 GFEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMI 421
           GFEIP AS Q+ IGLSI IS+P Y+R+ + +AR  T KPSGITMLQRIG GM++S L+M+
Sbjct: 367 GFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMV 426

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           VAA+VE+KRL+TA+E+GLVD P AT+P+SIWW VPQY+L G+ DVF++VG QEFFYDQVP
Sbjct: 427 VAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVP 486

Query: 482 SGLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRSIGL+  LS  G+ +FLS FLI+ I   T  +G +SWF+ NLN+AH+DYFYWLLA 
Sbjct: 487 TELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAA 546

Query: 542 LSAIGLAAFLCVARTYIYNK 559
            +AIG  AFL ++R Y+Y +
Sbjct: 547 FTAIGFLAFLLLSRLYVYRR 560

BLAST of Spg020950 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.1e-165
Identity = 302/557 (54.22%), Postives = 402/557 (72.17%), Query Frame = 0

Query: 8   ETVEGAVDYSGRPVRRSNSGGWRSASL-IIVEAAERFAYYGVSSNLINFLTDQLHQSTAT 67
           E VE +VD+ G P  RS+SG W+S+   +  E AE+FAY+G++SNLI + T+ L +STA 
Sbjct: 17  ENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAV 76

Query: 68  AAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPSTSA 127
           AA NVN W G A+ LPL+   +ADSFLGR+RTI L+S+ YI+GLGLLT SAT+P    S 
Sbjct: 77  AASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SL 136

Query: 128 CQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAKSSF 187
           C   E   +C  S V+++ FF +LYL+A  +GG K C++AFGADQFD Q P E+KAKSS+
Sbjct: 137 CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSY 196

Query: 188 FNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSNITD 247
           FNW YF +S+  L T  + NYVQ+NLSW+LG+ I C +M+ A  +FLLG +TYR+S   +
Sbjct: 197 FNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGE 256

Query: 248 EK------NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVAP 307
            +      NP VRIGRVF+ A RN     S+         LLP+ ++++ RFLD+A++  
Sbjct: 257 GRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCL------LLPNESTKKFRFLDRAVI-- 316

Query: 308 NGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPGF 367
                   +C   EVEEAKAVL L PIW+  L + IVF+Q  TFFTKQG TMDRSI    
Sbjct: 317 --------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTL 376

Query: 368 EIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIVA 427
           ++PAA+LQ FI L+I++ +PIYDR+ + IAR+ T KP+GIT LQRI  G+ +S++SM++A
Sbjct: 377 QVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIA 436

Query: 428 AVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSG 487
           A+VE+KRLKTA+++GLVD PKATVP+S+ WL+PQY+LFGV+DVFTMVGLQEFFY +VP  
Sbjct: 437 ALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQ 496

Query: 488 LRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLS 547
           LRS+GL+ YLSI G+GNFLSSF++S IE  TS  G+ SWF NNLN+AHLDYFYWLLA LS
Sbjct: 497 LRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLS 552

Query: 548 AIGLAAFLCVARTYIYN 558
           ++     +  A++Y+YN
Sbjct: 557 SLAFIFTVYFAKSYLYN 552

BLAST of Spg020950 vs. ExPASy TrEMBL
Match: A0A6J1IG54 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 6.7e-275
Identity = 483/562 (85.94%), Postives = 518/562 (92.17%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LII VE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SP  S CQ TEKS+ CSP L Q++ FF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW V SSEIAHEEEIRGLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN SKE  H CS+ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           F++PAASLQSFI LSIV+SL IYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF++NLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SAIGLAAFLC ARTYIY+KGNT
Sbjct: 541 SAIGLAAFLCFARTYIYSKGNT 562

BLAST of Spg020950 vs. ExPASy TrEMBL
Match: A0A6J1EHW2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 483/562 (85.94%), Postives = 517/562 (91.99%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVE AVDY+GRPVRRS+SGGWRSA+LII VE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQ TEKS+ CSP L Q++ FFFSLY+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW V SSEIAHEEEIRGLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALLV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN SKE  H C++ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           F++PAASLQSFI LSIV+SLPIYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FDVPAASLQSFISLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SAIGLAAFLC ARTYIY+K NT
Sbjct: 541 SAIGLAAFLCFARTYIYSKKNT 562

BLAST of Spg020950 vs. ExPASy TrEMBL
Match: A0A6J1EC56 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.4e-274
Identity = 481/562 (85.59%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY GRPVRRS+SGGWRSA+LII VE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVN WS AASLLPLLGAFLADSFLGRYRTIALSS LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQ TEKS+ CSP L Q++ FF S+Y+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLAT ATVNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+ N  DE+NP VRIGRVFITAVRNW V SSEIAHEEEI GLLPHH+SQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           P  SKE  H C++ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGVTM+RSIVPG
Sbjct: 301 PYSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           F++PAASLQSFIGLSIV+SLPIYDRILI IARNFTGKPSGITMLQRIGFGML+S LSM++
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFGVGNFLSSFLISAIE L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNT 562
           SA+GLAAFLC ARTYIY KGNT
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNT 562

BLAST of Spg020950 vs. ExPASy TrEMBL
Match: A0A1S3ATI3 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 4.5e-271
Identity = 477/563 (84.72%), Postives = 513/563 (91.12%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           MEVPLL+ETVEGAVDY+G PV RSNSGGWRSASLII VE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LSSALYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPSTSACQ TEKS+ CSP+L Q++ FFFSLYLV  AQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           + +KSSFFNWWYFG+SLAT ATVNILNYVQDNLSWSLGFGI C AMV A +VF LGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+SN  DE+NP VRIGRVFITAV+NW V SSE+A EEEIRGLLPHH+S+QLRFLDKAL+A
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN  KED  ACS+ EVEEAKAVLRL PIWVTCLA+AIVFSQ STFFTKQGVTMDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           FE+PAASLQSFI L+IVISL IYDRILI IARNFTGKPSGIT LQRIGFGML+S +SM++
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFG+GNFLSSFLISAIE+LTSG+G++SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNTI 563
           SA+GLAAFL   RTYIYNKGNT+
Sbjct: 541 SALGLAAFLFSTRTYIYNKGNTM 563

BLAST of Spg020950 vs. ExPASy TrEMBL
Match: A0A5A7TJ76 (Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00110 PE=3 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 4.5e-271
Identity = 477/563 (84.72%), Postives = 513/563 (91.12%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQ 60
           MEVPLL+ETVEGAVDY+G PV RSNSGGWRSASLII VE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LSSALYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 121 PSPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPSTSACQ TEKS+ CSP+L Q++ FFFSLYLV  AQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           + +KSSFFNWWYFG+SLAT ATVNILNYVQDNLSWSLGFGI C AMV A +VF LGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 241 RYSNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVA 300
           R+SN  DE+NP VRIGRVFITAV+NW V SSE+A EEEIRGLLPHH+S+QLRFLDKAL+A
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 301 PNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPG 360
           PN  KED  ACS+ EVEEAKAVLRL PIWVTCLA+AIVFSQ STFFTKQGVTMDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 361 FEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIV 420
           FE+PAASLQSFI L+IVISL IYDRILI IARNFTGKPSGIT LQRIGFGML+S +SM++
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 481 GLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRSIGLS YLSIFG+GNFLSSFLISAIE+LTSG+G++SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

Query: 541 SAIGLAAFLCVARTYIYNKGNTI 563
           SA+GLAAFL   RTYIYNKGNT+
Sbjct: 541 SALGLAAFLFSTRTYIYNKGNTM 563

BLAST of Spg020950 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 715.7 bits (1846), Expect = 2.9e-206
Identity = 368/559 (65.83%), Postives = 446/559 (79.79%), Query Frame = 0

Query: 4   PLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQLHQ 63
           PLL  T    VDY  +P  +S+SGGWRSA  II VE AERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSP 123
           STA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +SALYI+GLG+LT+SA +PS 
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSD 132

Query: 124 STSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKA 183
               C+ +    +CSP   Q+++FF +LYLVA AQGGHKPCVQAFGADQFD + PEE KA
Sbjct: 133 ----CKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKA 192

Query: 184 KSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYS 243
           KSSFFNWWYFG+   TL T+ +LNY+QDNLSW+LGFGI C AMV A +V LLGT TYR+S
Sbjct: 193 KSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFS 252

Query: 244 NITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVAPNG 303
              ++++P VRIG V++ AV+NW V + ++A  EE  GL+   +SQQ  FL+KALVA NG
Sbjct: 253 IRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNG 312

Query: 304 SKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPGFEI 363
           S      CS+ E+EEAK+VLRLAPIW+TCL YA+VF+Q  TFFTKQG TM+RSI PG++I
Sbjct: 313 S------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKI 372

Query: 364 PAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIVAAV 423
             A+LQSFI LSIVI +PIYDR+LI IAR+FT KP GITMLQRIG G+ +S L+M+VAA+
Sbjct: 373 SPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAAL 432

Query: 424 VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 483
           VE+KRLKTA +YGLVD P ATVP+S+WWLVPQYVLFG+ DVF MVGLQEFFYDQVP+ LR
Sbjct: 433 VEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELR 492

Query: 484 SIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 543
           S+GL+ YLSIFG+GNFLSSF+IS IE+ TS  G+ SWF NNLN+AHLDYFYWLLA LS I
Sbjct: 493 SVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFI 552

Query: 544 GLAAFLCVARTYIYNKGNT 562
           GLA++L VA++Y+  + +T
Sbjct: 553 GLASYLYVAKSYVSKRLDT 556

BLAST of Spg020950 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 604.4 bits (1557), Expect = 9.5e-173
Identity = 309/560 (55.18%), Postives = 405/560 (72.32%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQL 61
           E+ L EE V  AVD+ G   RRSN+G WR+A  II VE AERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            +STA AA NVNAWSG A+LLP+LGAF+AD+FLGRYRTI +SS +Y+LGL  LT+SA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFL- 125

Query: 122 SPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
                   P    V  S S    V FFFSLYLVA  Q GHKPCVQAFGADQFD +  +E 
Sbjct: 126 -------IPNTTEVTSSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 182 KAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
             +SSFFNWWY  LS      + ++ Y+Q+  SW+ GFGI C  MV + ++F+ G R YR
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 242 YSNITDEK--NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALV 301
           YS    E+  NP  RIGRVF  A++N  + SS++   E    L  + + ++  F +KAL+
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALL 305

Query: 302 APNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVP 361
            PN S +  +A    +VE+A A++RL P+W T LAYAI ++Q+ TFFTKQGVTMDR+I+P
Sbjct: 306 VPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILP 365

Query: 362 GFEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMI 421
           G +IP ASLQ FIG+SIV+ +PIYDR+ + IAR  T +P GIT L+RIG G+++S ++M+
Sbjct: 366 GVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMV 425

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           +AA+VE KRL+TA+E+GL+D P+AT+P+SIWWL+PQY+L G+ADV+T+VG+QEFFY QVP
Sbjct: 426 IAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVP 485

Query: 482 SGLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRSIGL+ YLS  GVG+ LSS LIS I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAI 545

Query: 542 LSAIGLAAFLCVARTYIYNK 559
           +SA+G   FL ++++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 553

BLAST of Spg020950 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 604.0 bits (1556), Expect = 1.2e-172
Identity = 309/561 (55.08%), Postives = 403/561 (71.84%), Query Frame = 0

Query: 5   LLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQLHQS 64
           L EE V  AVD+ G   RRS +G WR+A  II VE AERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPS 124
           TA AA NVNAWSG +++LPLLGAF+AD+FLGRY TI ++S +Y+LGL  LT+SA L    
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFL---- 128

Query: 125 TSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAK 184
                P    V  SPS      FFFSLYLVA  Q GHKPCVQAFGADQFD ++P+EN  +
Sbjct: 129 ----IPNNTEVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDR 188

Query: 185 SSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSN 244
           SSFFNWWY  +       + ++ Y+Q+N+SW+LGFGI C  MV + ++F+LG ++YR+S 
Sbjct: 189 SSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSK 248

Query: 245 I--TDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVAPN 304
               +E NP  RIGRVF  A +N  + SS++   E I       + ++L FL+KAL+ PN
Sbjct: 249 TRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPN 308

Query: 305 GSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPGFE 364
            S E   AC   +VE+A A++RL P+W+T LAYAI F+Q+ TFFTKQGVTM+R+I PG E
Sbjct: 309 DSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVE 368

Query: 365 IPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIVAA 424
           IP ASLQ  I +SIV+ +PIYDR+L+ I R+ T  P GIT L+RIG GM+++ L+M+VAA
Sbjct: 369 IPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAA 428

Query: 425 VVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 484
           +VE KRL+TA+EYGL+D PK T+P+SIWWL PQY+L G+ADV T+VG+QEFFY QVP+ L
Sbjct: 429 LVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTEL 488

Query: 485 RSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSA 544
           RS+GL+ YLS  GVG+ LSS LI  I+  T GD   SWF++NLN+AHLDYFYWLLA +SA
Sbjct: 489 RSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSA 548

Query: 545 IGLAAFLCVARTYIYNKGNTI 563
           +G   FL ++++YIY + + +
Sbjct: 549 VGFFTFLFISKSYIYRRVDVV 561

BLAST of Spg020950 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 588.2 bits (1515), Expect = 7.1e-168
Identity = 304/560 (54.29%), Postives = 400/560 (71.43%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLII-VEAAERFAYYGVSSNLINFLTDQL 61
           E  L+E++V  +VD+ G P  +S++GGWRSA  II VE  ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S +YILGLGLLT+S+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 122 SPSTSACQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
               S  +   ++ +  P     + FF SLYLVA  QGGHKPCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 182 KAKSSFFNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
            ++ SFFNWW+  LS     ++ ++ YVQDN++W+LGFGI C  MV A  +FL G +TYR
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 242 Y--SNITDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALV 301
           Y   +   + N   RIGRVF+ A +N  +K   + H  ++          QL FL KAL+
Sbjct: 247 YPRGDREGKNNAFARIGRVFLVAFKNRKLK---LTHSGQLEVGSYKKCKGQLEFLAKALL 306

Query: 302 APNGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVP 361
              G  E    CS  +VE+A A++RL PIW+T +   I ++Q++TFFTKQGVT+DR I+P
Sbjct: 307 PGEGGVE---PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILP 366

Query: 362 GFEIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMI 421
           GFEIP AS Q+ IGLSI IS+P Y+R+ + +AR  T KPSGITMLQRIG GM++S L+M+
Sbjct: 367 GFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMV 426

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           VAA+VE+KRL+TA+E+GLVD P AT+P+SIWW VPQY+L G+ DVF++VG QEFFYDQVP
Sbjct: 427 VAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVP 486

Query: 482 SGLRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRSIGL+  LS  G+ +FLS FLI+ I   T  +G +SWF+ NLN+AH+DYFYWLLA 
Sbjct: 487 TELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAA 546

Query: 542 LSAIGLAAFLCVARTYIYNK 559
            +AIG  AFL ++R Y+Y +
Sbjct: 547 FTAIGFLAFLLLSRLYVYRR 560

BLAST of Spg020950 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 584.7 bits (1506), Expect = 7.8e-167
Identity = 302/557 (54.22%), Postives = 402/557 (72.17%), Query Frame = 0

Query: 8   ETVEGAVDYSGRPVRRSNSGGWRSASL-IIVEAAERFAYYGVSSNLINFLTDQLHQSTAT 67
           E VE +VD+ G P  RS+SG W+S+   +  E AE+FAY+G++SNLI + T+ L +STA 
Sbjct: 17  ENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAV 76

Query: 68  AAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPSTSA 127
           AA NVN W G A+ LPL+   +ADSFLGR+RTI L+S+ YI+GLGLLT SAT+P    S 
Sbjct: 77  AASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SL 136

Query: 128 CQPTEKSVACSPSLVQLVSFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAKSSF 187
           C   E   +C  S V+++ FF +LYL+A  +GG K C++AFGADQFD Q P E+KAKSS+
Sbjct: 137 CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSY 196

Query: 188 FNWWYFGLSLATLATVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSNITD 247
           FNW YF +S+  L T  + NYVQ+NLSW+LG+ I C +M+ A  +FLLG +TYR+S   +
Sbjct: 197 FNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGE 256

Query: 248 EK------NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHTSQQLRFLDKALVAP 307
            +      NP VRIGRVF+ A RN     S+         LLP+ ++++ RFLD+A++  
Sbjct: 257 GRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCL------LLPNESTKKFRFLDRAVI-- 316

Query: 308 NGSKEDSHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVTMDRSIVPGF 367
                   +C   EVEEAKAVL L PIW+  L + IVF+Q  TFFTKQG TMDRSI    
Sbjct: 317 --------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTL 376

Query: 368 EIPAASLQSFIGLSIVISLPIYDRILILIARNFTGKPSGITMLQRIGFGMLVSVLSMIVA 427
           ++PAA+LQ FI L+I++ +PIYDR+ + IAR+ T KP+GIT LQRI  G+ +S++SM++A
Sbjct: 377 QVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIA 436

Query: 428 AVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSG 487
           A+VE+KRLKTA+++GLVD PKATVP+S+ WL+PQY+LFGV+DVFTMVGLQEFFY +VP  
Sbjct: 437 ALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQ 496

Query: 488 LRSIGLSPYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLS 547
           LRS+GL+ YLSI G+GNFLSSF++S IE  TS  G+ SWF NNLN+AHLDYFYWLLA LS
Sbjct: 497 LRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLS 552

Query: 548 AIGLAAFLCVARTYIYN 558
           ++     +  A++Y+YN
Sbjct: 557 SLAFIFTVYFAKSYLYN 552

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023535812.11.1e-27686.12protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo][more]
XP_023535811.11.9e-27685.94protein NRT1/ PTR FAMILY 5.10-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_023535809.11.3e-27586.12protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_023535815.11.1e-27485.59protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo][more]
XP_022973964.11.4e-27485.94protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q0WP014.1e-20565.83Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q8VZE21.3e-17155.18Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q0WSZ61.8e-17155.08Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q9SK969.9e-16754.29Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Q9C7U11.1e-16554.22Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1IG546.7e-27585.94protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 P... [more]
A0A6J1EHW21.1e-27485.94protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710... [more]
A0A6J1EC567.4e-27485.59protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731... [more]
A0A1S3ATI34.5e-27184.72protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 ... [more]
A0A5A7TJ764.5e-27184.72Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT1G22540.12.9e-20665.83Major facilitator superfamily protein [more]
AT1G72120.19.5e-17355.18Major facilitator superfamily protein [more]
AT1G72125.11.2e-17255.08Major facilitator superfamily protein [more]
AT1G22550.17.1e-16854.29Major facilitator superfamily protein [more]
AT1G72140.17.8e-16754.22Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 5..561
e-value: 1.1E-193
score: 646.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 29..552
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 96..512
e-value: 8.2E-92
score: 308.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 16..559
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 16..559
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 85..109
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 29..556
e-value: 0.0
score: 691.317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg020950.1Spg020950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity