Spg020948 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg020948
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationscaffold9: 394836 .. 395510 (+)
RNA-Seq ExpressionSpg020948
SyntenySpg020948
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAAGGTTGCCCGCCGTTTCGCCAAAGAACTACGCGCCGTCGTAATCTCTGTCAACTACCGCCTCGCTCCGGAGTTCCGATTCCCTTGCCAATACGACGACGGATTCGACGCCTTGAAATTCATCGACGAAATGGACGACGACGCATTGCCGGCGAGGGCAGATCTGAGCCGTTGCTTCCTTTTGGGGGAAAGCGCCGGGGGGAATCTGGGGCACCACGTGGCGGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTCGGACTCATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGCTGCCGTTGACTCTGAAAATAACGGATTGGTTCTGGAAGGCGTTTTTGTCGGCCGGAGAAGATCGAGACCACACGACGGTGAATGTGAATGGACCAAATGAAAGGGATCTTTCGGGATTGAGGAAGTTTCCGGCGACGTTGATTTTCGCCGGAGGATTAGATCTGCTGATAGATCGGCGGAGACGGTATTCTAAGGGGCTGAAGAGAATGGGAAAGGGTGTGGAGCTGGTGGAATTTTCGAACAGCGTCCATGGATTCTTTGGCTTCACAGATCTGCCGGAATATTCTTTGATGATCAAGAAGATGAGGGATTTCATTGCCAAGCTGAGATAG

mRNA sequence

ATGGACAAGGTTGCCCGCCGTTTCGCCAAAGAACTACGCGCCGTCGTAATCTCTGTCAACTACCGCCTCGCTCCGGAGTTCCGATTCCCTTGCCAATACGACGACGGATTCGACGCCTTGAAATTCATCGACGAAATGGACGACGACGCATTGCCGGCGAGGGCAGATCTGAGCCGTTGCTTCCTTTTGGGGGAAAGCGCCGGGGGGAATCTGGGGCACCACGTGGCGGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTCGGACTCATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGCTGCCGTTGACTCTGAAAATAACGGATTGGTTCTGGAAGGCGTTTTTGTCGGCCGGAGAAGATCGAGACCACACGACGGTGAATGTGAATGGACCAAATGAAAGGGATCTTTCGGGATTGAGGAAGTTTCCGGCGACGTTGATTTTCGCCGGAGGATTAGATCTGCTGATAGATCGGCGGAGACGGTATTCTAAGGGGCTGAAGAGAATGGGAAAGGGTGTGGAGCTGGTGGAATTTTCGAACAGCGTCCATGGATTCTTTGGCTTCACAGATCTGCCGGAATATTCTTTGATGATCAAGAAGATGAGGGATTTCATTGCCAAGCTGAGATAG

Coding sequence (CDS)

ATGGACAAGGTTGCCCGCCGTTTCGCCAAAGAACTACGCGCCGTCGTAATCTCTGTCAACTACCGCCTCGCTCCGGAGTTCCGATTCCCTTGCCAATACGACGACGGATTCGACGCCTTGAAATTCATCGACGAAATGGACGACGACGCATTGCCGGCGAGGGCAGATCTGAGCCGTTGCTTCCTTTTGGGGGAAAGCGCCGGGGGGAATCTGGGGCACCACGTGGCGGTGAGAGCGAGCGAGTACGAGTTCAAGAGAGTGAAGGTGGTCGGACTCATAGCGTCGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGCTGCCGTTGACTCTGAAAATAACGGATTGGTTCTGGAAGGCGTTTTTGTCGGCCGGAGAAGATCGAGACCACACGACGGTGAATGTGAATGGACCAAATGAAAGGGATCTTTCGGGATTGAGGAAGTTTCCGGCGACGTTGATTTTCGCCGGAGGATTAGATCTGCTGATAGATCGGCGGAGACGGTATTCTAAGGGGCTGAAGAGAATGGGAAAGGGTGTGGAGCTGGTGGAATTTTCGAACAGCGTCCATGGATTCTTTGGCTTCACAGATCTGCCGGAATATTCTTTGATGATCAAGAAGATGAGGGATTTCATTGCCAAGCTGAGATAG

Protein sequence

MDKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCFLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLKITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKGLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIAKLR
Homology
BLAST of Spg020948 vs. NCBI nr
Match: XP_008459713.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 379.4 bits (973), Expect = 2.1e-101
Identity = 181/221 (81.90%), Postives = 197/221 (89.14%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCF 61
           D  ARRFAK+LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDD+L  R DLS CF
Sbjct: 117 DMAARRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCF 176

Query: 62  LLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLKI 121
           +LGESAGGNLGHHVAVRASEYEFKRVK++G IASQPFFGG+ERTESE RL  QLPLTL +
Sbjct: 177 ILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDM 236

Query: 122 TDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKGL 181
           TDWFW+AFL AGEDRDH   NVNGPN RD+SGL  FPATLIFAGGLDLLIDR++ Y + L
Sbjct: 237 TDWFWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERL 296

Query: 182 KRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIAK 223
           KRMGK V+LV FSN+VHG+FGF DLPEYSLMIK+MRDFIAK
Sbjct: 297 KRMGKDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of Spg020948 vs. NCBI nr
Match: KAA0039340.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 375.9 bits (964), Expect = 2.4e-100
Identity = 180/221 (81.45%), Postives = 196/221 (88.69%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCF 61
           D  ARRFAK+L AVVISVNYRLAPEFRFPCQ DDGFD LKFIDEMDDD+L  R DLSRCF
Sbjct: 117 DMAARRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCF 176

Query: 62  LLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLKI 121
           +LGESAGGNLGHHVAVRASEYEFKRVK++G IASQPFFGG+ERTESE RL  QLPLTL +
Sbjct: 177 ILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDM 236

Query: 122 TDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKGL 181
           TDWFW+AFL AGEDRDH T NVNGPN RD+SGL  FPATLIFAGGLDLLIDR++ Y + L
Sbjct: 237 TDWFWRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERL 296

Query: 182 KRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIAK 223
           KRMGK V+LV FSN+VHG+FGF DLPEYSLMIK+MRDFIAK
Sbjct: 297 KRMGKDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of Spg020948 vs. NCBI nr
Match: XP_023536262.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375.9 bits (964), Expect = 2.4e-100
Identity = 181/222 (81.53%), Postives = 196/222 (88.29%), Query Frame = 0

Query: 1   MDKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDDALPARADLSR 60
           MD  ARRF+KEL AV+ISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DDD L  +ADLSR
Sbjct: 121 MDMAARRFSKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 180

Query: 61  CFLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTL 120
           CF+LGESAGGNLGHHVAVRASEY FKRVKV+G IASQPFFGGEERTESEIRL NQ PLTL
Sbjct: 181 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 240

Query: 121 KITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSK 180
           K+ DWFWKAFL AGEDRDH   NVNGPN RD+SGLR FPAT+I AGGLDLLIDR+RRY +
Sbjct: 241 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 300

Query: 181 GLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIA 222
            LKRMGK V+LV F+N VHGFF F DLPEYSLMIK+MRDFIA
Sbjct: 301 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 342

BLAST of Spg020948 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 375.6 bits (963), Expect = 3.1e-100
Identity = 181/222 (81.53%), Postives = 196/222 (88.29%), Query Frame = 0

Query: 1   MDKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDDALPARADLSR 60
           MD  ARRF+KEL AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DDD L  +ADLSR
Sbjct: 113 MDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 172

Query: 61  CFLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTL 120
           CF+LGESAGGNLGHHVAVRASEY FKRVKV+G IASQPFFGGEERTESEIRL NQ PLTL
Sbjct: 173 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 232

Query: 121 KITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSK 180
           K+ DWFWKAFL AGEDRDH   NVNGPN RD+SGLR FPAT+I AGGLD+LIDR+RRY +
Sbjct: 233 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYE 292

Query: 181 GLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIA 222
            LKRMGK V+LV F+N VHGFF F DLPEYSLMIK+MRDFIA
Sbjct: 293 ELKRMGKEVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 334

BLAST of Spg020948 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 373.6 bits (958), Expect = 1.2e-99
Identity = 181/222 (81.53%), Postives = 195/222 (87.84%), Query Frame = 0

Query: 1   MDKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDDALPARADLSR 60
           MD  ARRF+KEL AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DDD L  +ADLSR
Sbjct: 113 MDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 172

Query: 61  CFLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTL 120
           CF+LGESAGGNLGHHVAVRASEY FKRVKV+G IASQPFFGGEERTESEIRL NQ PLTL
Sbjct: 173 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 232

Query: 121 KITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSK 180
           K+ DWFWKAFL AGEDRDH   NVNGPN RD+SGLR FPAT+I AGGLDLLIDR+RRY +
Sbjct: 233 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 292

Query: 181 GLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIA 222
            LKRMGK V+LV F+N VHGFF F D PEYSLMIK+MRDFIA
Sbjct: 293 ELKRMGKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of Spg020948 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 8.0e-59
Identity = 116/222 (52.25%), Postives = 148/222 (66.67%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCF 61
           D V RRFA++L A VISVNYRLAPE R+P QYDDGFDALK+I+E     LPA ADLSRCF
Sbjct: 109 DNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCF 168

Query: 62  LLGESAGGNLGHHVAV---RASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLT 121
             G+SAGGN+ H+VA+   R     F  VK++GLI+ QPFFGGEERTE+E +L     ++
Sbjct: 169 FAGDSAGGNIAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVS 228

Query: 122 LKITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYS 181
              TDW WKA    G +RDH  VNV GPN  D+SGL  +P T++   G D L D +R Y 
Sbjct: 229 PDRTDWCWKAM---GLNRDHEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYY 288

Query: 182 KGLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
           + LK  GK   L+E+ N  H F+ F +LPE   +I +++DF+
Sbjct: 289 EWLKLCGKKATLIEYPNMFHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Spg020948 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.8e-45
Identity = 98/220 (44.55%), Postives = 135/220 (61.36%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADL-SRC 61
           D + RRF K  + VV+SVNYR APE R+PC YDDG+ ALK++  M    + +  D  +R 
Sbjct: 135 DSLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARV 194

Query: 62  FLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLK 121
           FL G+S+GGN+ HHVAVRA++   + VKV G I     FGG ERTESE RL  +  +TL+
Sbjct: 195 FLSGDSSGGNIAHHVAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQ 254

Query: 122 ITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKG 181
             DW+WKA+L    DRDH   N  GPN R L GL  F  +LI   GLDL  DR+  Y+  
Sbjct: 255 DRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGL-PFAKSLIIVSGLDLTCDRQLAYADA 314

Query: 182 LKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
           L+  G  V++V+  N+  GF+   +   Y  +++++ DF+
Sbjct: 315 LREDGHHVKVVQCENATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Spg020948 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 4.0e-42
Identity = 91/220 (41.36%), Postives = 135/220 (61.36%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLS-RC 61
           D + RR      AVV+SVNYR APE R+PC YDDG+  LK+++      L ++ D   R 
Sbjct: 126 DTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRI 185

Query: 62  FLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLK 121
           FL G+S+GGN+ H+VAVRA E    R+ V+G I   P FGG ERTESE RL  +  +T++
Sbjct: 186 FLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVR 245

Query: 122 ITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKG 181
             DW+W+AFL  GEDR+H   +  GP  + L GL  FP +L+   GLDL+ D + +Y++G
Sbjct: 246 DRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEG 305

Query: 182 LKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
           LK+ G+ V+L+    +  GF+   +   +  ++ ++  F+
Sbjct: 306 LKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Spg020948 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 9.2e-39
Identity = 84/201 (41.79%), Postives = 121/201 (60.20%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLS-RC 61
           D + RR     + VV+SVNYR APE  +PC YDDG+ AL +++      L ++ D     
Sbjct: 128 DTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHI 187

Query: 62  FLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLK 121
           FL G+S+GGN+ H+VA+RA E     + V+G I   P FGG ERTESE  L  +  +T++
Sbjct: 188 FLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVR 247

Query: 122 ITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKG 181
             DW+WKAFL  GEDR+H   N   P  + L G+  FP +L+   GLDL+ D +  Y++G
Sbjct: 248 DRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEG 307

Query: 182 LKRMGKGVELVEFSNSVHGFF 202
           LK+ G+ V+L+    +  GF+
Sbjct: 308 LKKAGQEVKLMHLEKATVGFY 322

BLAST of Spg020948 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 4.5e-38
Identity = 84/220 (38.18%), Postives = 129/220 (58.64%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLS-RC 61
           D   RR       VV+SV+YR +PE R+PC YDDG++AL ++       L +  D +   
Sbjct: 128 DTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSR--VWLQSGKDSNVYV 187

Query: 62  FLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLK 121
           +L G+S+GGN+ H+VAVRA+    + VKV+G I   P FGG+ERT+SE  L  +  +T++
Sbjct: 188 YLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQ 247

Query: 122 ITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKG 181
             DW+W+A+L  GEDRDH   N  GP  + L G+  FP +L+   GLDL+ D +  Y  G
Sbjct: 248 DRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDG 307

Query: 182 LKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
           LK+ G  V L+    +  GF+   +   +  +++++  F+
Sbjct: 308 LKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of Spg020948 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.0e-101
Identity = 181/221 (81.90%), Postives = 197/221 (89.14%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCF 61
           D  ARRFAK+LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDD+L  R DLS CF
Sbjct: 117 DMAARRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCF 176

Query: 62  LLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLKI 121
           +LGESAGGNLGHHVAVRASEYEFKRVK++G IASQPFFGG+ERTESE RL  QLPLTL +
Sbjct: 177 ILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDM 236

Query: 122 TDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKGL 181
           TDWFW+AFL AGEDRDH   NVNGPN RD+SGL  FPATLIFAGGLDLLIDR++ Y + L
Sbjct: 237 TDWFWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERL 296

Query: 182 KRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIAK 223
           KRMGK V+LV FSN+VHG+FGF DLPEYSLMIK+MRDFIAK
Sbjct: 297 KRMGKDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of Spg020948 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.1e-100
Identity = 180/221 (81.45%), Postives = 196/221 (88.69%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCF 61
           D  ARRFAK+L AVVISVNYRLAPEFRFPCQ DDGFD LKFIDEMDDD+L  R DLSRCF
Sbjct: 117 DMAARRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCF 176

Query: 62  LLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLKI 121
           +LGESAGGNLGHHVAVRASEYEFKRVK++G IASQPFFGG+ERTESE RL  QLPLTL +
Sbjct: 177 ILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDM 236

Query: 122 TDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKGL 181
           TDWFW+AFL AGEDRDH T NVNGPN RD+SGL  FPATLIFAGGLDLLIDR++ Y + L
Sbjct: 237 TDWFWRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERL 296

Query: 182 KRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIAK 223
           KRMGK V+LV FSN+VHG+FGF DLPEYSLMIK+MRDFIAK
Sbjct: 297 KRMGKDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of Spg020948 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.7e-100
Identity = 181/222 (81.53%), Postives = 195/222 (87.84%), Query Frame = 0

Query: 1   MDKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDDALPARADLSR 60
           MD  ARRF+KEL AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DDD L  +ADLSR
Sbjct: 113 MDMAARRFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSR 172

Query: 61  CFLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTL 120
           CF+LGESAGGNLGHHVAVRASEY FKRVKV+G IASQPFFGGEERTESEIRL NQ PLTL
Sbjct: 173 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 232

Query: 121 KITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSK 180
           K+ DWFWKAFL AGEDRDH   NVNGPN RD+SGLR FPAT+I AGGLDLLIDR+RRY +
Sbjct: 233 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYE 292

Query: 181 GLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIA 222
            LKRMGK V+LV F+N VHGFF F D PEYSLMIK+MRDFIA
Sbjct: 293 ELKRMGKEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of Spg020948 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 7.4e-100
Identity = 180/221 (81.45%), Postives = 194/221 (87.78%), Query Frame = 0

Query: 1   MDKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD-DALPARADLSR 60
           MD  ARRF+KEL AVVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD D L  RADLSR
Sbjct: 113 MDMAARRFSKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSR 172

Query: 61  CFLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTL 120
           CF+LGESAGGNLGHHVAVRASEY FKRVKV+G IASQPFFGGEERTESEIRL NQ PLTL
Sbjct: 173 CFILGESAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTL 232

Query: 121 KITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSK 180
           K+ DWFWKAFL AGEDRDH   NVNGPN RD+SGLR FPAT+I AGGLD LIDR+RRY +
Sbjct: 233 KLADWFWKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYE 292

Query: 181 GLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
            LKRMGK V+LV F+N VHGFF F DLPEYSLMIK+MRDFI
Sbjct: 293 ELKRMGKDVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of Spg020948 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.3e-99
Identity = 177/222 (79.73%), Postives = 196/222 (88.29%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCF 61
           D  AR FA++LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDD+L  R DLSRCF
Sbjct: 117 DMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCF 176

Query: 62  LLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLKI 121
           +LGESAGGNLGHHVAVRASEYEFKRVK++G IASQPFFGG+ERTESE RL  QLPLTL +
Sbjct: 177 ILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYM 236

Query: 122 TDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKGL 181
           TDWFW+AFL AGEDRDH   NVNGPN RD+SGL  FPAT+IFAGGLDLL+DR++ Y + L
Sbjct: 237 TDWFWRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERL 296

Query: 182 KRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFIAKL 224
           KRMGK V+LV FSN+ HGFFGF DLPEYSLMI++M DFIAKL
Sbjct: 297 KRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of Spg020948 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 228.4 bits (581), Expect = 5.7e-60
Identity = 116/222 (52.25%), Postives = 148/222 (66.67%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCF 61
           D V RRFA++L A VISVNYRLAPE R+P QYDDGFDALK+I+E     LPA ADLSRCF
Sbjct: 109 DNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCF 168

Query: 62  LLGESAGGNLGHHVAV---RASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLT 121
             G+SAGGN+ H+VA+   R     F  VK++GLI+ QPFFGGEERTE+E +L     ++
Sbjct: 169 FAGDSAGGNIAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVS 228

Query: 122 LKITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYS 181
              TDW WKA    G +RDH  VNV GPN  D+SGL  +P T++   G D L D +R Y 
Sbjct: 229 PDRTDWCWKAM---GLNRDHEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYY 288

Query: 182 KGLKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
           + LK  GK   L+E+ N  H F+ F +LPE   +I +++DF+
Sbjct: 289 EWLKLCGKKATLIEYPNMFHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Spg020948 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.8e-43
Identity = 91/220 (41.36%), Postives = 135/220 (61.36%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLS-RC 61
           D + RR      AVV+SVNYR APE R+PC YDDG+  LK+++      L ++ D   R 
Sbjct: 126 DTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRI 185

Query: 62  FLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLK 121
           FL G+S+GGN+ H+VAVRA E    R+ V+G I   P FGG ERTESE RL  +  +T++
Sbjct: 186 FLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVR 245

Query: 122 ITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKG 181
             DW+W+AFL  GEDR+H   +  GP  + L GL  FP +L+   GLDL+ D + +Y++G
Sbjct: 246 DRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEG 305

Query: 182 LKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
           LK+ G+ V+L+    +  GF+   +   +  ++ ++  F+
Sbjct: 306 LKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Spg020948 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 6.5e-40
Identity = 84/201 (41.79%), Postives = 121/201 (60.20%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLS-RC 61
           D + RR     + VV+SVNYR APE  +PC YDDG+ AL +++      L ++ D     
Sbjct: 128 DTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHI 187

Query: 62  FLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLK 121
           FL G+S+GGN+ H+VA+RA E     + V+G I   P FGG ERTESE  L  +  +T++
Sbjct: 188 FLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVR 247

Query: 122 ITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKG 181
             DW+WKAFL  GEDR+H   N   P  + L G+  FP +L+   GLDL+ D +  Y++G
Sbjct: 248 DRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEG 307

Query: 182 LKRMGKGVELVEFSNSVHGFF 202
           LK+ G+ V+L+    +  GF+
Sbjct: 308 LKKAGQEVKLMHLEKATVGFY 322

BLAST of Spg020948 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 3.2e-39
Identity = 84/220 (38.18%), Postives = 129/220 (58.64%), Query Frame = 0

Query: 2   DKVARRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLS-RC 61
           D   RR       VV+SV+YR +PE R+PC YDDG++AL ++       L +  D +   
Sbjct: 128 DTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSR--VWLQSGKDSNVYV 187

Query: 62  FLLGESAGGNLGHHVAVRASEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLK 121
           +L G+S+GGN+ H+VAVRA+    + VKV+G I   P FGG+ERT+SE  L  +  +T++
Sbjct: 188 YLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQ 247

Query: 122 ITDWFWKAFLSAGEDRDHTTVNVNGPNERDLSGLRKFPATLIFAGGLDLLIDRRRRYSKG 181
             DW+W+A+L  GEDRDH   N  GP  + L G+  FP +L+   GLDL+ D +  Y  G
Sbjct: 248 DRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDG 307

Query: 182 LKRMGKGVELVEFSNSVHGFFGFTDLPEYSLMIKKMRDFI 221
           LK+ G  V L+    +  GF+   +   +  +++++  F+
Sbjct: 308 LKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of Spg020948 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 143.7 bits (361), Expect = 1.8e-34
Identity = 83/197 (42.13%), Postives = 115/197 (58.38%), Query Frame = 0

Query: 9   AKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDALPARADLSRCFLLGESAG 68
           A++L A+V+S +YRLAPE R P  YDDG +AL +I   DD+ + + AD S  FL+G SAG
Sbjct: 109 ARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDWIKTSDDEWIKSHADFSNVFLMGTSAG 168

Query: 69  GNLGHHVAVRA--SEYEFKRVKVVGLIASQPFFGGEERTESEIRLRNQLPLTLKITDWFW 128
           GNL ++V +R+  S  +   +++ GLI   PFFGGEER+ESEIRL N       +TD  W
Sbjct: 169 GNLAYNVGLRSVDSVSDLSPLQIRGLILHHPFFGGEERSESEIRLMNDQVCPPIVTDVMW 228

Query: 129 KAFLSAGEDRDHTTVNVN-GPNERDLSGLRKFPATLIFAGGL-DLLIDRRRRYSKGLKRM 188
              L  G DRDH   N   G     L  + +    ++  GG  D +ID ++  +K +K+ 
Sbjct: 229 DLSLPVGVDRDHEYSNPTVGDGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKK- 288

Query: 189 GKGVELVEF--SNSVHG 200
            KGVE+VE      VHG
Sbjct: 289 -KGVEVVEHYTGGHVHG 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008459713.12.1e-10181.90PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
KAA0039340.12.4e-10081.45putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00523.1 putative car... [more]
XP_023536262.12.4e-10081.53probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
KAG6592331.13.1e-10081.53putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022925491.11.2e-9981.53probable carboxylesterase 18 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LT108.0e-5952.25Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q6L5453.8e-4544.55Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q940G64.0e-4241.36Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q9MAA79.2e-3941.79Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC14.5e-3838.18Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CBA41.0e-10181.90probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1[more]
A0A5D3BL911.1e-10081.45Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1ECC85.7e-10081.53probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ... [more]
A0A6J1IER77.4e-10081.45probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV... [more]
A0A0A0KXS81.3e-9979.73Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23530.15.7e-6052.25carboxyesterase 18 [more]
AT5G27320.12.8e-4341.36alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.16.5e-4041.79alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.13.2e-3938.18alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.11.8e-3442.13carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..223
e-value: 1.7E-58
score: 200.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..222
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 2..201
e-value: 6.8E-46
score: 156.8
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 1..221
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 1..221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg020948.1Spg020948.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity