Spg020896 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg020896 vs. NCBI nr
Match: KAG6592376.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 666.4 bits (1718), Expect = 2.1e-187
Identity = 362/542 (66.79%), Postives = 427/542 (78.78%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHEL 60
           MAGTS  N V+E+ +LLRRD L++ALETPTSIF LPDS+TK++PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGMNWVVEVGELLRRDGLRMALETPTSIFMLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRI 120
             ELHKMEIFKLE AK  KE+FQLPDLT MV+QLKE EV IRA YG NLA+GS+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPDLTLMVDQLKELEVPIRASYGANLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITT-TQNEI 180
           LIDGLFL+RLL I + +  ++    +     +++ELPVFNE++LYL LE N+ T TQNEI
Sbjct: 121 LIDGLFLIRLLQIFADEKNDQGSSDRLHNRRHKLELPVFNEKELYLKLERNMATMTQNEI 180

Query: 181 VKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDE---EDDIPFD 240
           +KDLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L P +D+    DD P  
Sbjct: 181 LKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSL-PSADKFTTSDDNP-- 240

Query: 241 LFPDYRRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATI 300
                   F  L EILD SQHL+HF YALL+YQRF AY      RS ++ VELLNL+A +
Sbjct: 241 --------FLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKV 300

Query: 301 LGIGLLKQLVEALGLIQTLIGFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGG 360
           + +G+++QLVEA+GLIQ+LIG +GK+  SSP ET+ SP FPSA+KL+KVGVKF  GDGGG
Sbjct: 301 VQVGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGG 360

Query: 361 FDEESTTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTIL 420
           FD+ES TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YVKLM+GLIAT KDV IL
Sbjct: 361 FDDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGIL 420

Query: 421 KRAGIIAGGRSGDEDDVVKMFKELGNVVNVSS-DDGESRANFKWYMNFEVDIIEKIKVYY 480
           KRAGII GGRSGDEDDVVK+FK+LGNVV     D  E R+NF+ YMNFE  I E+I  YY
Sbjct: 421 KRAGIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRIGEEIMKYY 480

Query: 481 ESRWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIINSGYY 538
           ESRW+VKAERF KRYI PV Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II+S Y 
Sbjct: 481 ESRWMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYD 531

BLAST of Spg020896 vs. NCBI nr
Match: XP_022925375.1 (putative UPF0481 protein At3g02645 [Cucurbita moschata])

HSP 1 Score: 664.1 bits (1712), Expect = 1.0e-186
Identity = 360/539 (66.79%), Postives = 426/539 (79.04%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LLRRD L++ALETPTSIFKLPDS+TK++PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRI 120
             ELHKMEIFKLE AK  KE+FQLP+LT MV+QLKE EV IRA YG  LA+GS+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITT-TQNEI 180
           LIDGLFL+RLL I + +  ++    +     +++ELPVF+EQ+LYL LEGN+ T T+NEI
Sbjct: 121 LIDGLFLIRLLQIFADEKNDQGSSDRLHNRRHKLELPVFDEQELYLKLEGNMATMTRNEI 180

Query: 181 VKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFP 240
           +KDLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L P +D+     F    
Sbjct: 181 LKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSL-PSADK-----FTTSN 240

Query: 241 DYRRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATILGI 300
           D    F  L EILD SQHL+HFLYALL+YQRF AY      RS ++ VELLNL+A ++ +
Sbjct: 241 D--NPFLNLSEILDQSQHLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVVQV 300

Query: 301 GLLKQLVEALGLIQTLIGFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGGFDE 360
           G+++QLVEA+GLIQ+LIG +GK+  SSP ET+ SP  PSA KL+KVGVKF  GDGGGFD+
Sbjct: 301 GIVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGLPSAMKLRKVGVKFHGGDGGGFDD 360

Query: 361 ESTTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRA 420
           ES TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YV LM+GLIAT KDV ILKRA
Sbjct: 361 ESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILKRA 420

Query: 421 GIIAGGRSGDEDDVVKMFKELGNVVNVSS-DDGESRANFKWYMNFEVDIIEKIKVYYESR 480
           GII GGRSGDEDDVVK+FK+LGNVV     D  E R+NF+ YMNFE  I E+I  YYESR
Sbjct: 421 GIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRISEEIMKYYESR 480

Query: 481 WVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIINSGYYFL 538
           W+VKAERF KRYI PV Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II+S Y  L
Sbjct: 481 WMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYDML 531

BLAST of Spg020896 vs. NCBI nr
Match: XP_022973973.1 (UPF0481 protein At3g47200-like [Cucurbita maxima])

HSP 1 Score: 640.6 bits (1651), Expect = 1.2e-179
Identity = 356/542 (65.68%), Postives = 423/542 (78.04%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHEL 60
           MA TS TN V+E+ +LLRRD L++ALETPTSIFKLPDS+T  +PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAETSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVT--NPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRI 120
             ELHKMEIFKLE AK  KE+FQLP+LT MV+QLKE EV IRA YG NLA+ S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIDSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSEL---PYEVELPVFNEQQLYLNLEGNITT-TQ 180
           LIDGLFL++LL I +    EK ++G +  L    +++ELPVFNEQ+LYL LEGN+ T T+
Sbjct: 121 LIDGLFLIQLLQISA---DEKNDEGSSDWLHNRRHKLELPVFNEQELYLKLEGNMATLTR 180

Query: 181 NEIVKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFD 240
           NEI+KDLLKLENQIPIQ+LKRVLP++  ENL + FYKFCV VSPL L P +D+     F 
Sbjct: 181 NEILKDLLKLENQIPIQVLKRVLPKDFTENLPILFYKFCVFVSPLSL-PSADK-----FT 240

Query: 241 LFPDYRRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATI 300
              D    F  L EILD SQHL+HF YALL+YQRF AY      RS ++ VELLNL+A +
Sbjct: 241 TSND--NVFLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKV 300

Query: 301 LGIGLLKQLVEALGLIQTLIGFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGG 360
           + +G+++QLVEA+GLIQ+LIG +GK+  SSP ET+ SP FPSA+KL+KVGVKF  GDGGG
Sbjct: 301 VQVGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGG 360

Query: 361 FDEESTTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTIL 420
           F++ES TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YVKLM+GLIAT KDV IL
Sbjct: 361 FNDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGIL 420

Query: 421 KRAGIIAGGRSGDEDDVVKMFKELGN-VVNVSSDDGESRANFKWYMNFEVDIIEKIKVYY 480
           KRAGII  GRSGDEDDVVK+FK+LGN VV    D  E R+NF+ +MNFE  I E+I  YY
Sbjct: 421 KRAGIIVSGRSGDEDDVVKLFKDLGNVVVKGGFDSEEGRSNFESFMNFERRIGEEIVKYY 480

Query: 481 ESRWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIINSGYY 538
           E RW+VK ERF KRY+ PV Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II+S Y 
Sbjct: 481 EGRWMVKGERFVKRYVFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYD 529

BLAST of Spg020896 vs. NCBI nr
Match: XP_023535329.1 (putative UPF0481 protein At3g02645 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 613.2 bits (1580), Expect = 2.1e-171
Identity = 350/564 (62.06%), Postives = 415/564 (73.58%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LLRRD L++ALETPTSIFKLPDS+TK++PQ+YSPSRL LGPFHEL
Sbjct: 1   MAGTSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYSPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRI 120
             ELHKMEIFKLE AK  KE+FQLP+LT MV+QLKE EV IRA YG NLA+GS+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIGSDALASRI 120

Query: 121 LIDGLFLLRLL----------GIISPDDKEKE----------EKGKNSELP--------- 180
           LIDGLFL+ LL          GI+     E E            G+   +P         
Sbjct: 121 LIDGLFLIWLLQIYADEKNYPGILQGLSTEDELGPKIRPKGVVDGQQVNIPVLPLVGPEG 180

Query: 181 --------------YEVELPVFNEQQLYLNLEGNITT-TQNEIVKDLLKLENQIPIQILK 240
                         Y++ELPVF+EQ+LYL LEGN+ T T+NEI+KDLLKLENQIPIQ+LK
Sbjct: 181 RRRLGSSDRLHNRRYKLELPVFDEQELYLKLEGNMATMTRNEILKDLLKLENQIPIQVLK 240

Query: 241 RVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDYRRSFPKLPEILDGSQ 300
           RVLP++L+ENL + FYKFCV VSPL L P +D+     F    D    F  L EILD SQ
Sbjct: 241 RVLPKDLIENLPILFYKFCVFVSPLSL-PSADK-----FTTSND--NPFLNLSEILDQSQ 300

Query: 301 HLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATILGIGLLKQLVEALGLIQTLI 360
           HL+HFLYALL+YQRF AY      RS ++ VELLNL+A ++ +G+++QLVEA+GLIQ+LI
Sbjct: 301 HLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVVQVGIVQQLVEAVGLIQSLI 360

Query: 361 GFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGGFDEESTTLKLPTITMNAFSE 420
           G +GK+  SSP ET+ SP  PSA+KL+KVGVKF  GDGGGFD+ES TL LP++T+N F E
Sbjct: 361 GMLGKIHHSSPFETETSPGLPSATKLRKVGVKFHGGDGGGFDDESATLTLPSVTINDFYE 420

Query: 421 VILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIIAGGRSGDEDDVVKM 480
           VILRNLVAFEVEAK+NP  LS YV LM+GLIAT KDV ILKRAGII GGRSGDEDDVVK+
Sbjct: 421 VILRNLVAFEVEAKMNPSSLSHYVTLMNGLIATTKDVGILKRAGIIVGGRSGDEDDVVKL 480

Query: 481 FKELGN-VVNVSSDDGESRANFKWYMNFEVDIIEKIKVYYESRWVVKAERFGKRYIVPVF 520
           FK+LGN VV    D  E R+NF+ +MNFE  I E+I  YYESRW+VKAERF KRYI PV 
Sbjct: 481 FKDLGNVVVKGGFDSEEGRSNFESFMNFEGRIGEEIMKYYESRWMVKAERFVKRYIFPVL 540

BLAST of Spg020896 vs. NCBI nr
Match: KAG7025194.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 576.2 bits (1484), Expect = 2.8e-160
Identity = 330/526 (62.74%), Postives = 385/526 (73.19%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LLRRD L++ALETPTSIF LPDS+TK++PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFMLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRI 120
             ELHKMEIFKLE AK  KE+FQLPDLT MV+QLKE EV IRA YG  LA+GS+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPDLTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIV 180
           LIDGLFL+RLL I + D+K                    N+Q                  
Sbjct: 121 LIDGLFLIRLLQIFA-DEK--------------------NDQ------------------ 180

Query: 181 KDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDE---EDDIPFDL 240
            DLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L P +D+    DD P   
Sbjct: 181 -DLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSL-PSADKFTTSDDNP--- 240

Query: 241 FPDYRRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATIL 300
                  F  L EILD SQHL+HF YALL+YQRF AY      RS ++ VELLNL+A ++
Sbjct: 241 -------FLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKVV 300

Query: 301 GIGLLKQLVEALGLIQTLIGFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGGF 360
            +G+++QLVEA+GLIQ+LIG +GK+  SSP ET+ SP FPSA+KL+KVGVKF  GDGGGF
Sbjct: 301 QVGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGGF 360

Query: 361 DEESTTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILK 420
           D+ES TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YV LM+GLIAT KDV ILK
Sbjct: 361 DDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILK 420

Query: 421 RAGIIAGGRSGDEDDVVKMFKELGNVVNVSS-DDGESRANFKWYMNFEVDIIEKIKVYYE 480
           RAGII GGRSGDEDDVVK+FK+LGNVV     D  E R+NF+ YMNFE  I E+I  YYE
Sbjct: 421 RAGIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRIGEEIMKYYE 475

Query: 481 SRWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCP 523
           SRW+VKAERF KRYI PV Q L+ILV VVLILIVA+RTVC     P
Sbjct: 481 SRWMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCELHKTP 475

BLAST of Spg020896 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 7.8e-36
Identity = 144/552 (26.09%), Postives = 241/552 (43.66%), Query Frame = 0

Query: 8   NCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHELKPELHKM 67
           N    +D  L   DL+   E   SIF +P +L    P SY+P R+ +GP+H LKPELH+M
Sbjct: 24  NVQKSLDAELEEHDLE---EVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEM 83

Query: 68  EIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRILIDGLFL 127
           E +KL  A+K + ++       +VE+L+  E++IRACY   +    E L W + +D  FL
Sbjct: 84  ERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFL 143

Query: 128 LRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIVKDLLKLE 187
           +  L I S                       F + +  +N  G+     NEI++D++ +E
Sbjct: 144 IEFLKIYS-----------------------FRKVETLINRVGH-----NEILRDIMMIE 203

Query: 188 NQIPIQILKRVLPRNL-----VENLHLSFYK-FCVSVSPLPLFPGSDEEDDIPFDLFPDY 247
           NQIP+ +L++ L   L      ++L LS     C  +SPL           I FD     
Sbjct: 204 NQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLV----------IKFDDDQIL 263

Query: 248 RRSFPKLPEILDGSQHLVHFLYALLFYQ----------RFSAYEQRGSERSLNILVELLN 307
           +  F +   ILD       FLY ++  +            +  ++ G  R++  + E+ +
Sbjct: 264 KAQFQECNHILD-------FLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKH 323

Query: 308 --------------------LMATILGIGLLKQLVEALGLIQTLIGFIGKVQQSSPSETD 367
                               +++ + G   LK   + L   Q       + +  S  + +
Sbjct: 324 QFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIE 383

Query: 368 GSP-----RFPSASKLKKVGVKFDAGDGGG-----FDEESTTLKLPTITMNAFSEVILRN 427
             P       PS S L K GV+F     G      FD  S    LP I ++  +E +LRN
Sbjct: 384 KPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRN 443

Query: 428 LVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIIAGGRSGDEDDVVKMFKELG 487
           LVA+E      P   + Y +L++G+I + +DV +L+  G++      D+ +  +M+  + 
Sbjct: 444 LVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQ-EAAEMWNGMS 503

Query: 488 NVVNVSSDDGESRANFKWYMNFEVDIIEKIKVYYESRWVVKAERFGKRYIVPVFQALLIL 514
             V ++             + F    IE +  YY  RW VK  R  + Y+   +Q L  L
Sbjct: 504 KSVRLTK------------VGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFL 514

BLAST of Spg020896 vs. ExPASy TrEMBL
Match: A0A6J1EHS6 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111432682 PE=4 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 5.0e-187
Identity = 360/539 (66.79%), Postives = 426/539 (79.04%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHEL 60
           MAGTS TN V+E+ +LLRRD L++ALETPTSIFKLPDS+TK++PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAGTSGTNWVVEVGELLRRDGLRMALETPTSIFKLPDSVTKENPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRI 120
             ELHKMEIFKLE AK  KE+FQLP+LT MV+QLKE EV IRA YG  LA+GS+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGTKLAIGSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITT-TQNEI 180
           LIDGLFL+RLL I + +  ++    +     +++ELPVF+EQ+LYL LEGN+ T T+NEI
Sbjct: 121 LIDGLFLIRLLQIFADEKNDQGSSDRLHNRRHKLELPVFDEQELYLKLEGNMATMTRNEI 180

Query: 181 VKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFP 240
           +KDLLKLENQIPIQ+LKRVLP++L+ENL + FYKFCV VSPL L P +D+     F    
Sbjct: 181 LKDLLKLENQIPIQVLKRVLPKDLIENLPILFYKFCVFVSPLSL-PSADK-----FTTSN 240

Query: 241 DYRRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATILGI 300
           D    F  L EILD SQHL+HFLYALL+YQRF AY      RS ++ VELLNL+A ++ +
Sbjct: 241 D--NPFLNLSEILDQSQHLLHFLYALLYYQRFLAYRPSQDYRSGSLFVELLNLLAKVVQV 300

Query: 301 GLLKQLVEALGLIQTLIGFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGGFDE 360
           G+++QLVEA+GLIQ+LIG +GK+  SSP ET+ SP  PSA KL+KVGVKF  GDGGGFD+
Sbjct: 301 GIVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGLPSAMKLRKVGVKFHGGDGGGFDD 360

Query: 361 ESTTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRA 420
           ES TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YV LM+GLIAT KDV ILKRA
Sbjct: 361 ESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVTLMNGLIATTKDVGILKRA 420

Query: 421 GIIAGGRSGDEDDVVKMFKELGNVVNVSS-DDGESRANFKWYMNFEVDIIEKIKVYYESR 480
           GII GGRSGDEDDVVK+FK+LGNVV     D  E R+NF+ YMNFE  I E+I  YYESR
Sbjct: 421 GIIVGGRSGDEDDVVKLFKDLGNVVMKGGFDSEEGRSNFESYMNFERRISEEIMKYYESR 480

Query: 481 WVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIINSGYYFL 538
           W+VKAERF KRYI PV Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II+S Y  L
Sbjct: 481 WMVKAERFVKRYIFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYDML 531

BLAST of Spg020896 vs. ExPASy TrEMBL
Match: A0A6J1IEU8 (UPF0481 protein At3g47200-like OS=Cucurbita maxima OX=3661 GN=LOC111472588 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 6.0e-180
Identity = 356/542 (65.68%), Postives = 423/542 (78.04%), Query Frame = 0

Query: 1   MAGTSRTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHEL 60
           MA TS TN V+E+ +LLRRD L++ALETPTSIFKLPDS+T  +PQ+Y+PSRL LGPFHEL
Sbjct: 1   MAETSETNWVVEVGELLRRDGLRMALETPTSIFKLPDSVT--NPQAYTPSRLLLGPFHEL 60

Query: 61  KPELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRI 120
             ELHKMEIFKLE AK  KE+FQLP+LT MV+QLKE EV IRA YG NLA+ S+ALA RI
Sbjct: 61  SHELHKMEIFKLEQAKVTKERFQLPELTLMVDQLKELEVPIRASYGANLAIDSDALASRI 120

Query: 121 LIDGLFLLRLLGIISPDDKEKEEKGKNSEL---PYEVELPVFNEQQLYLNLEGNITT-TQ 180
           LIDGLFL++LL I +    EK ++G +  L    +++ELPVFNEQ+LYL LEGN+ T T+
Sbjct: 121 LIDGLFLIQLLQISA---DEKNDEGSSDWLHNRRHKLELPVFNEQELYLKLEGNMATLTR 180

Query: 181 NEIVKDLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFD 240
           NEI+KDLLKLENQIPIQ+LKRVLP++  ENL + FYKFCV VSPL L P +D+     F 
Sbjct: 181 NEILKDLLKLENQIPIQVLKRVLPKDFTENLPILFYKFCVFVSPLSL-PSADK-----FT 240

Query: 241 LFPDYRRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATI 300
              D    F  L EILD SQHL+HF YALL+YQRF AY      RS ++ VELLNL+A +
Sbjct: 241 TSND--NVFLNLSEILDQSQHLLHFFYALLYYQRFLAYMPSQDYRSGSLFVELLNLLAKV 300

Query: 301 LGIGLLKQLVEALGLIQTLIGFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGG 360
           + +G+++QLVEA+GLIQ+LIG +GK+  SSP ET+ SP FPSA+KL+KVGVKF  GDGGG
Sbjct: 301 VQVGVVQQLVEAVGLIQSLIGMLGKIHHSSPFETETSPGFPSATKLRKVGVKFRGGDGGG 360

Query: 361 FDEESTTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTIL 420
           F++ES TL LP +T+N F EVILRNLVAFEVEAKLNP  LS YVKLM+GLIAT KDV IL
Sbjct: 361 FNDESATLTLPIVTINDFYEVILRNLVAFEVEAKLNPSSLSHYVKLMNGLIATTKDVGIL 420

Query: 421 KRAGIIAGGRSGDEDDVVKMFKELGN-VVNVSSDDGESRANFKWYMNFEVDIIEKIKVYY 480
           KRAGII  GRSGDEDDVVK+FK+LGN VV    D  E R+NF+ +MNFE  I E+I  YY
Sbjct: 421 KRAGIIVSGRSGDEDDVVKLFKDLGNVVVKGGFDSEEGRSNFESFMNFERRIGEEIVKYY 480

Query: 481 ESRWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIMGHSIINSGYY 538
           E RW+VK ERF KRY+ PV Q L+ILV VVLILIVA+RTVCGWFSCPKIMGH II+S Y 
Sbjct: 481 EGRWMVKGERFVKRYVFPVLQGLMILVVVVLILIVAIRTVCGWFSCPKIMGHRIISSAYD 529

BLAST of Spg020896 vs. ExPASy TrEMBL
Match: A0A6J1IEU4 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111472585 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 3.2e-109
Identity = 242/533 (45.40%), Postives = 342/533 (64.17%), Query Frame = 0

Query: 6   RTNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHELKPELH 65
           +TN V++I+QLL  ++LQ AL+TPTSIFKLP S+T   PQ+Y P  + LGP+H  K EL+
Sbjct: 14  KTNWVVQINQLLDPNNLQTALQTPTSIFKLPHSITNGMPQAYFPCHIALGPYHHFKNELY 73

Query: 66  KMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRILIDGL 125
           +ME++KL+NA  A E F+LP L Q+   L++F+++IR+CY   L  G+E LAW +LIDGL
Sbjct: 74  QMELYKLDNAINAIESFKLPQLHQIAADLEQFDIKIRSCYDKFLDFGAEVLAWIMLIDGL 133

Query: 126 FLLRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIVKDLLK 185
           FL++LL I + +  E     ++  LP     P+F EQ+LY +L      TQ+E+V D+LK
Sbjct: 134 FLVQLLAISNHEKDELRTMRQDLYLP--SPFPLFVEQKLYGSLIEANLMTQDELVNDILK 193

Query: 186 LENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDYRR-- 245
           LENQIPI +LK++LP+NL  NLH+ F++FC  VSP  L P             P + R  
Sbjct: 194 LENQIPIFVLKKILPQNLSNNLHILFFRFCEVVSPKKLPPHDS----------PQFMRYS 253

Query: 246 SFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSL-NILVELLNLMATILGIGLL 305
            +  L +IL+ S HL+HFLY L+     S     G      + LVELLN +A++L I LL
Sbjct: 254 GYTDLYQILEQSHHLLHFLYLLILDSSLSIDRPVGMALLRGSFLVELLNALASVLQIALL 313

Query: 306 KQLVEALGLIQTLIGFIGKVQQSSPS---ETDGSPRFPSASKLKKVGVKF-----DAGDG 365
           +QL EA+GLIQ+L+G +G+V  SS S   + +G P  PSA +L++VGVK      D  + 
Sbjct: 314 QQLSEAVGLIQSLLGLLGRVGSSSSSTGEDMNGPPLIPSAEQLRRVGVKLEGNYEDFHES 373

Query: 366 GGFDEE--STTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKD 425
             F++E     LKLPTI +NAFSEV+ RNLVAFE   KLNPPC S+YV L+SGLI T KD
Sbjct: 374 ISFEKERREPCLKLPTIVINAFSEVVYRNLVAFEGATKLNPPCFSNYVALISGLITTTKD 433

Query: 426 VTILKRAGIIAGGRSGDEDDVVKMFKELGNVVNVSSDDGESRANFKWYMNFEVDIIEKIK 485
           V ILK+A II    +G E++VVK+F  L +V+ + +               E+ +++ I 
Sbjct: 434 VKILKKASIIE-SHAGSEEEVVKLFDGLRSVLELKN-------------MAELQMVKDIN 493

Query: 486 VYYESRWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIM 526
            YYES W VKA+RF ++YI P+ + ++++V ++LI +V VRT CGWF C +I+
Sbjct: 494 SYYESCWKVKAKRFIQKYINPMLKVIVLVVVILLIGVVVVRTFCGWFGCSRIL 520

BLAST of Spg020896 vs. ExPASy TrEMBL
Match: A0A6J1DUS9 (putative UPF0481 protein At3g02645 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023673 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 4.7e-108
Identity = 241/528 (45.64%), Postives = 350/528 (66.29%), Query Frame = 0

Query: 10  VLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHELKPELHKMEI 69
           +++I QLL  ++LQ AL+TPTSIFK+ DS+TK++P++Y P R+ LGP+H  K +LHKM++
Sbjct: 35  LVQISQLLEPNNLQTALQTPTSIFKIADSITKNNPEAYFPRRVALGPYHHFKNDLHKMQL 94

Query: 70  FKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRILIDGLFLLR 129
           FKL N  KA+E F L +L+ ++++LK  E++IR+C+   L +GSEALA  +LIDGLFL++
Sbjct: 95  FKLHNTIKARETFHLQELSHIMDELKHLEIKIRSCFDHFLDIGSEALARIMLIDGLFLIQ 154

Query: 130 LLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNL-EGNITTTQNEIVKDLLKLEN 189
           LL +IS D+K+ E +    +L      P+F EQQLY +L EGN+  TQ+E VKD+LKLEN
Sbjct: 155 LL-VISTDEKD-ELRSARPDLCLPSPFPLFVEQQLYSHLIEGNL-MTQDEFVKDILKLEN 214

Query: 190 QIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDYRRSFPKL 249
           QIP+ +LK +LP N   NLHL F+KFC  VSP  L P      D+P DL       +  L
Sbjct: 215 QIPLFVLKTILPENFGNNLHLLFFKFCNFVSPKQLPP-----HDMP-DLMS--LSGYTNL 274

Query: 250 PEILDGSQHLVHFLYALLFYQRFSAYEQRGSER-SLNILVELLNLMATILGIGLLKQLVE 309
            +I+D S HL+HFLY L+  +R S  +         + LVELLN+++++L IG L+QL E
Sbjct: 275 SQIIDQSHHLLHFLYLLILDRRLSVDQAVAMALFRTSFLVELLNVLSSVLQIGFLQQLSE 334

Query: 310 ALGLIQTLIGFIGKVQQSSPS---ETDGSPRFPSASKLKKVGVKFDA-----GDGGGFDE 369
           A GL+Q+L G +G+V  SS S   + D  P  PSAS+LK+V VKFD       +   F++
Sbjct: 335 AAGLLQSLFGLLGRVGSSSSSSGEDMDSPPLIPSASELKRVRVKFDGRYNDFHESMRFEQ 394

Query: 370 ES--TTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILK 429
           ES    L++P++ +N FSEV+ RNLVAFE   K N    + YV LMSGLI TAKDV ILK
Sbjct: 395 ESKKVCLEMPSMAINVFSEVVFRNLVAFEAATKKNATWFAFYVALMSGLITTAKDVKILK 454

Query: 430 RAGIIAGGRSGDEDDVVKMFKELGNVVNVSSDDGESRANFKWYMNFEVDIIEKIKVYYES 489
           +A II     G E++VVK+F  L N++ + +    S ++ +     E+ I++ I  YYES
Sbjct: 455 QAEIIE-SHWGSEEEVVKLFDGLRNLLELHNQQ-MSYSSGRIMEMAEIQIVKDINFYYES 514

Query: 490 RWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIM 526
            W VKA+RF KR++ P+ + + +++ ++LI++V +RT CGWFSC +++
Sbjct: 515 CWKVKAKRFIKRFVNPILKVIFLILVILLIVVVVLRTSCGWFSCSRVL 549

BLAST of Spg020896 vs. ExPASy TrEMBL
Match: A0A6J1EBZ5 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111432681 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 3.9e-107
Identity = 238/532 (44.74%), Postives = 338/532 (63.53%), Query Frame = 0

Query: 7   TNCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHELKPELHK 66
           TN V++I+QLL  ++LQ AL+TPTSIFKLP+S+T   PQ+Y P  + LGP+H  + EL++
Sbjct: 15  TNWVVQINQLLDPNNLQTALQTPTSIFKLPNSITNSMPQAYFPRHIALGPYHHFQNELYQ 74

Query: 67  MEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRILIDGLF 126
           ME++KL+NA  A E F+LP L Q+   L++F+++IR+CY   L  G+EALAW +LIDGLF
Sbjct: 75  MELYKLDNAINAIESFKLPQLHQIAADLEQFDIKIRSCYNKFLDFGAEALAWIMLIDGLF 134

Query: 127 LLRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIVKDLLKL 186
           L+++L I + ++ E     ++  LP     P+F EQ+LY +L      TQ+E+V D+LKL
Sbjct: 135 LVQILAISNDENDELRRMRQDLYLP--SPFPLFVEQKLYCSLIEANLMTQDELVNDILKL 194

Query: 187 ENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDYRR--S 246
           ENQIP  +LK++LP+NL  NLH+ F++FC  VSP  L P             P + R   
Sbjct: 195 ENQIPSFVLKKILPQNLSNNLHILFFRFCEVVSPKKLPPHDS----------PQFMRYSG 254

Query: 247 FPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSL-NILVELLNLMATILGIGLLK 306
           +  L +IL+ S HL+HFLY L+     S     G      + LVELLN +A++L I  L+
Sbjct: 255 YTDLYQILEQSHHLLHFLYLLILDSSLSIDRPVGMALLRGSFLVELLNALASVLQIAFLQ 314

Query: 307 QLVEALGLIQTLIGFIGKVQQSSPS---ETDGSPRFPSASKLKKVGVKF-----DAGDGG 366
           QL EA+GLIQ+L+G   +V  SS S   + +G P  PSA +L++VGVK      D  +  
Sbjct: 315 QLSEAVGLIQSLLGLFRRVGSSSSSIGEDMNGPPLIPSAEQLRRVGVKLEGNYEDFHESI 374

Query: 367 GFDEE--STTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDV 426
            F++E   T LK PTI +NAFSEV+  NLVAFE   KLNPPC S+YV LMSGLI T KDV
Sbjct: 375 SFEKERRETCLKFPTIVINAFSEVVYSNLVAFEGATKLNPPCFSNYVALMSGLITTIKDV 434

Query: 427 TILKRAGIIAGGRSGDEDDVVKMFKELGNVVNVSSDDGESRANFKWYMNFEVDIIEKIKV 486
            ILK A II     G+E++VVK+F  L +V+ + +               E+ +++ I  
Sbjct: 435 KILKEASIIE-SHIGNEEEVVKLFDGLRSVLELKN-------------MAEIQMVKDINS 494

Query: 487 YYESRWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTVCGWFSCPKIM 526
           YYES W VKA+RF ++YI P+ + ++++V ++LI +V VRT CGWF C +I+
Sbjct: 495 YYESCWKVKAKRFIQKYINPMLKVIVLVVVILLIGVVVVRTFCGWFGCLRIL 520

BLAST of Spg020896 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 153.3 bits (386), Expect = 5.6e-37
Identity = 144/552 (26.09%), Postives = 241/552 (43.66%), Query Frame = 0

Query: 8   NCVLEIDQLLRRDDLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHELKPELHKM 67
           N    +D  L   DL+   E   SIF +P +L    P SY+P R+ +GP+H LKPELH+M
Sbjct: 24  NVQKSLDAELEEHDLE---EVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEM 83

Query: 68  EIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRILIDGLFL 127
           E +KL  A+K + ++       +VE+L+  E++IRACY   +    E L W + +D  FL
Sbjct: 84  ERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFL 143

Query: 128 LRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIVKDLLKLE 187
           +  L I S                       F + +  +N  G+     NEI++D++ +E
Sbjct: 144 IEFLKIYS-----------------------FRKVETLINRVGH-----NEILRDIMMIE 203

Query: 188 NQIPIQILKRVLPRNL-----VENLHLSFYK-FCVSVSPLPLFPGSDEEDDIPFDLFPDY 247
           NQIP+ +L++ L   L      ++L LS     C  +SPL           I FD     
Sbjct: 204 NQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLV----------IKFDDDQIL 263

Query: 248 RRSFPKLPEILDGSQHLVHFLYALLFYQ----------RFSAYEQRGSERSLNILVELLN 307
           +  F +   ILD       FLY ++  +            +  ++ G  R++  + E+ +
Sbjct: 264 KAQFQECNHILD-------FLYQMIVPRIEEEELEEDDEENRADENGGNRAIRFMDEIKH 323

Query: 308 --------------------LMATILGIGLLKQLVEALGLIQTLIGFIGKVQQSSPSETD 367
                               +++ + G   LK   + L   Q       + +  S  + +
Sbjct: 324 QFKRVFASRPADLILRFPWRIISNLPGFMALKLSADYLFTRQENEATTTRQESVSILDIE 383

Query: 368 GSP-----RFPSASKLKKVGVKFDAGDGGG-----FDEESTTLKLPTITMNAFSEVILRN 427
             P       PS S L K GV+F     G      FD  S    LP I ++  +E +LRN
Sbjct: 384 KPPLVEELTIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRN 443

Query: 428 LVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIIAGGRSGDEDDVVKMFKELG 487
           LVA+E      P   + Y +L++G+I + +DV +L+  G++      D+ +  +M+  + 
Sbjct: 444 LVAYEATNTSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQ-EAAEMWNGMS 503

Query: 488 NVVNVSSDDGESRANFKWYMNFEVDIIEKIKVYYESRWVVKAERFGKRYIVPVFQALLIL 514
             V ++             + F    IE +  YY  RW VK  R  + Y+   +Q L  L
Sbjct: 504 KSVRLTK------------VGFLDKTIEDVNRYYTGRWKVKIGRLVEVYVYGSWQILAFL 514

BLAST of Spg020896 vs. TAIR 10
Match: AT5G22550.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 61.6 bits (148), Expect = 2.2e-09
Identity = 102/438 (23.29%), Postives = 174/438 (39.73%), Query Frame = 0

Query: 32  IFKLPDSLTKDSPQSYSPSRLFLGPFHELKPELHKMEIFKLENAKK-------AKEKFQL 91
           I+++P +L + + ++Y+P  + +GP+H    + H   +  +E  KK       +K K   
Sbjct: 46  IYRIPHTLKQVNDKAYAPKIVSIGPYHHSSDKQH---LKMIEEHKKRYLEMFVSKTKENG 105

Query: 92  PDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRILIDGLFLLRLLGIISPDDKEKEEK 151
             L  +V+ +   E +IR  Y  NL    + L   +L+DG F+L L  ++S         
Sbjct: 106 VYLIHLVDLVSGLEQKIRDSYSENLEFSQQKLIKVMLLDGCFILMLFLVVS--------- 165

Query: 152 GKNSELPY-EVELPVFNEQQLYLNLEGNITTTQNEIVKDLLKLENQIPIQILKRVLPRNL 211
               ++ Y  ++ P+F  + +   L             DLL LENQ+P+ +LK +L  + 
Sbjct: 166 ---QKIEYTNLKDPIFKLRWILPTLR-----------SDLLLLENQVPLFLLKVLLETSK 225

Query: 212 V---ENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDYRRSF-PKLPEILDGSQHLV 271
           +    +L++  +KF       P        +     L    R++F P  P      Q  +
Sbjct: 226 LAPSTSLNMLAFKFFDYSIKKPEGFWEKHNNLRAKHLLDLIRKTFIPAPPPSTTPRQCCI 285

Query: 272 HFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATILGIGLLKQLVEALGLIQTLIGFI 331
           +       Y R        +E S NI +  ++    I G                     
Sbjct: 286 NIFNGPREYSR--------TETSKNICLGKISCSKEITG--------------------- 345

Query: 332 GKVQQSSPSETDGSP---RFPSASKLKKVGVKFDAGDGG----GFDEESTTLKLPTITMN 391
              Q SSP      P      SA KL+  G+KF   +          +S  +++P +  +
Sbjct: 346 --AQTSSPPPPPRRPFLGLIVSARKLRLRGIKFMRKENVETPLDISFKSGLVEIPLLVFD 405

Query: 392 AFSEVILRNLVAFEVEAKLNPPC---LSDYVKLMSGLIATAKDVTILKRAGIIAGGRSGD 448
            F   +L N VAFE   + N  C   ++ +V  M  LI T  D T L   GI+     G 
Sbjct: 406 DFISNLLINCVAFE---QFNMSCSTEITSFVIFMGCLINTEDDATFLIEKGILE-NYFGT 422

BLAST of Spg020896 vs. TAIR 10
Match: AT3G60470.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 60.1 bits (144), Expect = 6.4e-09
Identity = 122/539 (22.63%), Postives = 224/539 (41.56%), Query Frame = 0

Query: 32  IFKLPDSLTKDSPQSYSPSRLFLGPFH-----------------ELKPELHKMEIFKLEN 91
           I+++PD L + +P++Y+P  L +GP H                 +L   L+  E  K+  
Sbjct: 34  IYRVPDRLRRVNPEAYTPQLLLIGPLHHSLKFQALNSRGDITNTKLMGYLNMEEHKKIYL 93

Query: 92  AKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLA-VGSEALAWRILIDGLFLLRLLGI 151
           A+ ++       + ++  ++KE E  IRA Y  + A + S      IL D +F++  L +
Sbjct: 94  ARFSERLAGQKTIDELRRRIKEEEDIIRASYSESTAWIESPKFVDMILHDSVFIIEFL-L 153

Query: 152 ISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIVKDLLKLENQIPIQ 211
            S D    E++G   +  ++   P+F +  L           Q  + +DL+ LENQ+P  
Sbjct: 154 RSKDYTLLEDQGSCIKKTWD---PIFEQPCL-----------QTTVDEDLILLENQLPYF 213

Query: 212 ILKRVL--------PRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDYRRSF 271
           IL+++         P   +  + +++++F  ++       G D       DLF   R   
Sbjct: 214 ILEKLFDPIVPILRPNQTLRQIVITYFRFQGNI-------GVDSRFRHFTDLFRCVRVET 273

Query: 272 --PKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNL----------M 331
             PK+P      ++   +L  L  +     +  R  E+S     + L +           
Sbjct: 274 LRPKMP------KNKFRYLVGLFRFVHLGTHGSRELEKSRPQEHKALRMEKTHGSGEPTK 333

Query: 332 ATILGIGLLKQLV--EALGLIQTLIGFIGKVQQSSPSETD---GSPR---------FPSA 391
           +T   I  L + V  E LG  +     + +++     ET    GS +           +A
Sbjct: 334 STFRYIADLFRCVPMETLGSKREEKSRLERLKTHRMEETSHGHGSKKTKELPIIKHMYNA 393

Query: 392 SKLKKVGVKFDA-GDGGGFDE--ESTTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCL 451
           +KL+  GVKF A  D    D   E+  LK+P + +   +E+ LRN++A E         +
Sbjct: 394 AKLQSAGVKFKAVTDEFSIDVTFENGCLKIPCLWVPDDAEITLRNIMALEQCHYPFKAYV 453

Query: 452 SDYVKLMSGLIATAKDVTILKRAGIIAGGRSGDEDDVVKMFKELGNVVNVSSDDGESRAN 511
            ++V  +  LI T KDV +L   GI+          V +M   L + V       ESR  
Sbjct: 454 CNFVSFLDFLIDTDKDVDLLMENGILNNWGEKSSVAVAEMVNTLFSGV------VESR-- 513

Query: 512 FKWYMNFEVDIIEKIKVYYESRWVVKAERFGKRYIVPVFQALLILVAVVLILIVAVRTV 516
                ++   I  ++  YYE+         G++Y   +++    + A +L+++  ++TV
Sbjct: 514 -----SYYAGIASRVNAYYENPVNRSRTILGRQYFGNLWRGTATVAAALLLVMTLIQTV 531

BLAST of Spg020896 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 54.3 bits (129), Expect = 3.5e-07
Identity = 94/496 (18.95%), Postives = 187/496 (37.70%), Query Frame = 0

Query: 25  ALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFHELKPELHKMEIFKLENAKKAKEKFQL 84
           +L T   I+K+P+ L + +P +Y+P  +  GP H  K EL  ME  K         +   
Sbjct: 288 SLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQKYRYLLSFIPRTN- 347

Query: 85  PDLTQMVEQLKEFEVQIRACYGGNLAVGSEALAWRILIDGLFLLRLLGIISPDDKEKEEK 144
             L  +V   + +E   R+CY  ++ + S+     +++DG FL+ LL            +
Sbjct: 348 SSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLVELL----LRSHYPRLR 407

Query: 145 GKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIVKDLLKLENQIPIQILKRVLPRNLV 204
           G+N          +F    +             ++ +D++ +ENQ+P  ++K +      
Sbjct: 408 GENDR--------IFGNSMMI-----------TDVCRDMILIENQLPFFVVKEIF----- 467

Query: 205 ENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDYRRSFPKLPEILDGSQHLVHFLYA 264
             L L++Y+                                   P I+  +Q   HF Y 
Sbjct: 468 -LLLLNYYQ--------------------------------QGTPSIIQLAQR--HFSYF 527

Query: 265 LLFYQRFSAYEQRGSERSLNILVELLNLMATILGIGLLKQLVEALGLIQTLIGFIGKVQQ 324
           L          +   E+ +      ++L+ +                   L  F  K++ 
Sbjct: 528 L---------SRIDDEKFITEPEHFVDLLRSCY-----------------LPQFPIKLEY 587

Query: 325 SSPSETDGSPRFPSASKLKKVGVKFDAGDGGG----FDEESTTLKLPTITMNAFSEVILR 384
           ++    +     P A++L   GV+F   +              LK+PTI ++  +E + +
Sbjct: 588 TTVKVDNA----PEATELHTAGVRFKPAETSSCLLDISFADGVLKIPTIVVDDLTESLYK 647

Query: 385 NLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGIIAGGRSGDEDDVVKMFKEL 444
           N++ FE + + +     DY+ L+   I +  D  +L  +GII     G+  DV  +F  +
Sbjct: 648 NIIGFE-QCRCSNKNFLDYIMLLGCFIKSPTDADLLIHSGIIV-NYLGNSVDVSNLFNSI 675

Query: 445 GNVVNVSSDDGESRANFKWYMNFEVDIIEKIKVYYESRWVVKAERFGKRYIVPVFQALLI 504
              V             ++Y +    + E ++ Y  + W        + Y    +    +
Sbjct: 708 SKEVIYDR---------RFYFSM---LSENLQAYCNTPWNRWKAILRRDYFHNPWAVASV 675

Query: 505 LVAVVLILIVAVRTVC 517
             A++L+L+  +++VC
Sbjct: 768 FAALLLLLLTFIQSVC 675

BLAST of Spg020896 vs. TAIR 10
Match: AT1G65985.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 51.2 bits (121), Expect = 3.0e-06
Identity = 113/514 (21.98%), Postives = 203/514 (39.49%), Query Frame = 0

Query: 21  DLQLALETPTSIFKLPDSLTKDSPQSYSPSRLFLGPFH------------------ELKP 80
           DL   ++ P  I+++P+ +    P++Y+P  + +GP H                   LK 
Sbjct: 6   DLDEKVQEP-CIYRVPNRMRVVKPEAYTPKVVVIGPLHRSLKSTAKDGAETSSNPWHLKK 65

Query: 81  ELHKMEIFKLENAKKAKEKFQLPDLTQMVEQLKEFEVQIRACYGGNLA-VGSEALAWRIL 140
           +  KME+ K    +   ++     + +M + ++  E  IR CY  +   + SE     IL
Sbjct: 66  DYIKMEVTKKTYFESFTKRVGEDTIEEMRKTIQTEEKNIRNCYEESTEWIPSELFVDLIL 125

Query: 141 IDGLFLLRLLGIISPDDKEKEEKGKNSELPYEVELPVFNEQQLYLNLEGNITTTQNEIVK 200
            D +F++ L+  +S  D+   +                       NL+  I      ++ 
Sbjct: 126 QDSIFIMELIIRLSGFDRIHSDD---------------------RNLDFAI------VMN 185

Query: 201 DLLKLENQIPIQILKRVLPRNLVENLHLSFYKFCVSVSPLPLFPGSDEEDDIPFDLFPDY 260
           DLL LENQ+P  IL  +   ++ + L        +    L  F    EE      L   Y
Sbjct: 186 DLLLLENQLPYFILDSLFSFSMTKYLD----SHTLDSFILRCFGLKIEEKKNFMHLTDMY 245

Query: 261 RRSFPKLPEILDGSQHLVHFLYALLFYQRFSAYEQRGSERSLNILVELLNLMATILGIGL 320
           R  + +  + +   ++ ++ +       ++S  E R +E   N  V   +       + L
Sbjct: 246 RCVYKESLDYIPPREYSLNRV-------KWSITELRNAENLSNAGVNFKSYDY----LDL 305

Query: 321 LKQLVEALGLIQTLIGFIGKVQQSSPSETDGSPRFPSASKLKKVGVKFDAGDGGGFDEES 380
            KQ  E L +  T        +  S SE     + P+  + +  GV          + + 
Sbjct: 306 SKQ-DEKLDIKIT--------RDKSDSEL---TKLPTNKRERVEGVSLHV------ELKK 365

Query: 381 TTLKLPTITMNAFSEVILRNLVAFEVEAKLNPPCLSDYVKLMSGLIATAKDVTILKRAGI 440
             L +P+   N  +++ILRN++AFE       P  S Y++ ++ LI + +DV +L   G+
Sbjct: 366 GCLSMPSFWANDRTDMILRNVIAFEQCQHGIIPFTSHYIQFLNALIVSDRDVELLSEKGV 425

Query: 441 IAGGRSGDEDDVVKMFKELGNVVNVSSDDGESRANFKWYMNFEVDIIEKIKVYYESRWVV 500
           +     G    VV M  +L   V            ++ Y +   DI   +K +Y+SR   
Sbjct: 426 VM-NNMGRPSLVVDMVNKLKVGV------------YEGYTSQYYDIAIDLKAHYKSRRKR 445

Query: 501 KAERFGKRYIVPVFQALLILVAVVLILIVAVRTV 516
                 K Y   ++     L AV+L+L+  V TV
Sbjct: 486 CWATLRKVYFSDLWTGTATLAAVLLLLLTLVGTV 445