Spg020777 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg020777
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Location	scaffold10: 27541595 .. 27545590 (+)
RNA-Seq Expression	Spg020777
Synteny	Spg020777

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGGACTTTGGTCACACTCCCAACACTCCATGTCCTGGGTGTCCACCTCAGTTTCTACTCCCTGATCACTACAGGATCCCTTCCGGCAAACAAATGGTTGCCCATTTCCGTCCATGCATGGTTGGTGCTCCTCCTAGAGAGAACCCGGAACCAGGACCAGGAGCATGTGTTCTGGAATTGTAAATTGGTAAGGTATATTTGGGGGAAAGTCTTTCCAATCCTAAATCAAATTTTTTTGATTTGCAGGGAATGGTGGAAATTCAAGGACTTTTGGTCCTGTGCTTTGAATCTGCTGGAGAAGCATGAAGCAGGGGTGGCTGGACACATTATTTGGGAGCTCTGGACCATGAGGAACAAATTGAAGCAGACCAACAACAGGCAAAATCTCACACAAATCTCAGAGGATATTTTCTATGCTATTGACGAATACATCTCAGAGATGGAGAATTCTAACCAGCACAGGGAGTCGGAGAACCTTCTGAGTCCCGATGTTTGGAAGCCGCCGGTTGACCTTCAGTGGAAGATGAACGTTGATGCGGCCTGGTTCGAAGCCTCGAATTCAGGGGGCCTGGGGTGGATCATTCGAGACTCAGTAGGATCTCTGATAGGAGCTGGAAGCAAGAAGATTGATAGATTTTCGGAGATTAAAATCTTGGAAGCCATGGCCATTATAGAAGGTCTTTCTAGCATCTTTGAAAGATCCAGATCTTACCCGGAAACGTTGGTGCACGACGTGGTGCTCGAATCTGATGCCGCCGAGGTCGTGAAGCTGTTTAATGGAGAAGTCGATGATCTATCGGATATCTCCTTCCTGATCGACGAAGCCCGTGAGATCGCGTCCAAGCTGTCTTTGGTCTCCTTCGTCCATTGCCCAAGGGAGTCGAATTTTTTGGCGCACTATCTGGCACGCAAAGTTGTTAGGAATTTTGGTTTTGTGCATTTCGTTTTTGATCCTTCTCTTTGCTCGAGAATGGATAGCCCTGCTGGTGAAGGGAATGTTTTACCCCTTTGGGCTACCTCCCTGTTGCGGGAGTTTGTTTGTACCTAACTTTGTGCTTTTCTTTATAATATTTCTCTTTAAAAAAAAAAATCATTTTGAAAAAAATTCTAGTGTTTGACAACCACTAAAAATGACTTATTAAATAATGAGATTAAAAATTAAACCATTTTGAAAAAAATACTTGAGAGCAAATTTTTTTAAAAGAGTTTTTAAGTATATAATAACTTGTGTATAAAAACACTGAAATGTAACCAAACATATGAGTGTTTAAATTCTAAACGATTTTTATATAAAATGTTTTTTTAAAAAAGACATTCTTGAAAAATATTTAAATTAAAATGTATTCCAAACGGACCCTTATTGTACTAAAAAGAAAACTAGAGTGCATCAAAAGTTGTTATAAAACTTTGAAATCACTTTTTCAATATCATAGTGAATAATAAATTCATTTAAAATCACTTTTCAAAACACATTTAATTTTGAGAGAAACACTTGAAACCAACTTTTTAAAAAGAGTTTTTAAGTATACAATAACTTGTGTATAAAAACACTTACGAGGAAATGTAACCAAACATATGAGTGTTTAAATTCTAAACAATTTTTAGATAAATTGTTTTTAAAAAATGCATTCTTGAAAAACATTTAAATTAAAATGTATTCCAAATGGACCCTTTGAATATTTTTTAGTTTAAGTGTTTTTCAAGATGCATTTTTTATAAACATTTTGTATAAAAATGGTTTAAAATTTAAATACTCATATGTTTGGTTACATGTCCTCATAAGTGCTTTTATGCACAAATTATTATATACTAAAAACTATTTTTAAAAAATTTGCCTTCAATGTTTCTCTCAAAATGATTTATTTTTCAATCTAATTTTTTAAGGCCCCGTTTGATAACCATTTCGTTTTTGGTTTTTGGTTTTTGAAAATTAAACCTATAAACACTATTTTTACCCATAACCTTCTTTGTTTTTTATGTACATCCTTTCCCATGTTTTAAAAAACCAAGCCAATTTTTGAAAACAAAAAAAAGTAGTTTTCAAAAACTTGTTTCTGTTTTTGGAATTTGGCTAGGAACTCAAATGTCTCCTTAACAAAAATGAAAATCATGGTAGGAAGATTGAGAGAAAACAAGCACAATTTTCAAAAACCAAAAACAAAAAACGAAATGGTTATCAAATGGGGTCTTAATAAGTCATTTTTAGTGGTTGCCAAACACTAGAATTTTTTTTCAAAATGATTTTTTTTTTAAAAAATTAAACACTTGAAAAGTTAAACCAAACACGCCCTTATAGGTTGTAACACCCATAATTTAAGGAAAATTCAAATCTCCTTAGTTTGGAGTTGATTTGGAGTTAAGATGAAAATTTTCAAAGATTAAAAGCAGGGAAATTGGTGGAGTTTTAATAATTCAAAGCTGGAGTGGAATTTTTGAAATTTAGGTGAATTAAGAGCCAAGCCCAAGCCCATTAGGTCTTCATTTAGCCACTCACGCAGCCCTAGGTTAGTACCTTCTTCATCCCTTCTTTCTTCTCCACGCAACTTCAACCCCCTTTCTTCTTCCTTCTCCACGAGCAGTCGCACAGGGGAGGGCTTCTTCGGCGCCTTCTTCTTCAATTTTTGGACGGCAGCGCGGCGTCCTCCAGCGACGGCAGGCAACGACGACAACTACTTCCGGCGGTAGCAGAACAGTCCGACGACGCGCGACACTTCCAGCCGAGTCTCCCTCTCTCTCTCTCTCGCGTCGACTGGTGCAGCGAAGGCCTGCGTTCGTCCTTCCGGTGCGTGGCGGCGTCGCGTGGGGAGCAGCAGCGAGCGGCGATTTCGTTTGTGGTTGTCTCTTCCGGCCGTGCAGTGTGTAGCAGTGATTTCACACGACCGGCAGCGTTCTCGGCGTGGGTGCATAGTGGGTCGGCGACCAGCAACTTCTTTCGACGATTTGAGGTTTTTTGAGTGTGGGTAAGCAAGATTTTTAGTCCCTCAATTTGTTTTGTTGTTGTGTTGAATGCCCATTGATTTGTGGCTTTGGTTATGAGTATCCTAAGCGAGTTCAAGGCGTTTTCCGACGGAGTTCGTGTGGGTATCTTCCTTTGGCATTTTCGGCGAATTTAGCTTTGGGTATGTGAGATTTAAGATTATTTGGACCAATTTCACTCACTAATCAAATACCCATTGATTTTAGTTTTCAATTTCAAGGTACTCATAGCGTTTGGAGGTTTAATCGTCGAATTTGAGCGATATTAGAACCCAACTAGCAAGCTTAAGTTCTGTTTTGGATCGATCATTTAATTAAGGTTGAGTTTGACCCTTTTGTTGAGCTGATTAGGCAGCTTAGAATAAAGACAATTAAGCCAAGGTAAGTGAACTTACTATTGAATCTTTTAATTGGGTGCGCGTGTTTGATTTTCCGAGATTCTCGTGCTTTATGATTTGTTTGATTATATTTTCATTTATGAAAATTATTGAAAGCATGAAATTTATTATTATTGCTTTTAATCGTTAATATTAAAATAATCGAGTTGGTGGTCTCTTGAAATTCCATTAAAGGAATTAGTTTAAAATTCCATTAAAGGAATTAGTTTAAGTTATTTATGAGTTTTATAAGTGTACATGAAATTGATGTACATTCCGAGATAGGACTTAGAGACATGAAATTGATGTACCTAAGTCGAGGTTCGTGAAATTGACGACCTCGAGGTTTCGTGAAATTGACCTCGAGGTTTCGTGAAATTGACGGCCTCCATAGAAGACACATGAAATTGATGTGTCTAGGATATGCATGAAATTGATGTATATCCTTAGTAAATTTAAAAATAAAGTTTCGAGCCACCAAAATTATGTTAGTTGTTGATTTTTAAAATGTTTTTAAAGCATGACCCAACCTCAATAGTAGGGTCACTTACTGAGTATTTAAAATACTCATTCTTTTCAAAAATATTTTTTTCAGGTAAAGGCAAGGGCAGATGAAAGGCGATTGATGAGAATCCGTTAG

mRNA sequence

ATGTGGGACTTTGGTCACACTCCCAACACTCCATGTCCTGGGTGTCCACCTCAGTTTCTACTCCCTGATCACTACAGGATCCCTTCCGGCAAACAAATGGTTGCCCATTTCCGTCCATGCATGGTTGGTGCTCCTCCTAGAGAGAACCCGGAACCAGGACCAGGAGCATGTGTTCTGGAATTGGAATGGTGGAAATTCAAGGACTTTTGGTCCTGTGCTTTGAATCTGCTGGAGAAGCATGAAGCAGGGGTGGCTGGACACATTATTTGGGAGCTCTGGACCATGAGGAACAAATTGAAGCAGACCAACAACAGGCAAAATCTCACACAAATCTCAGAGGATATTTTCTATGCTATTGACGAATACATCTCAGAGATGGAGAATTCTAACCAGCACAGGGAGTCGGAGAACCTTCTGAGTCCCGATGTTTGGAAGCCGCCGGTTGACCTTCAGTGGAAGATGAACGTTGATGCGGCCTGGTTCGAAGCCTCGAATTCAGGGGGCCTGGGGTGGATCATTCGAGACTCAGTAGGATCTCTGATAGGAGCTGGAAGCAAGAAGATTGATAGATTTTCGGAGATTAAAATCTTGGAAGCCATGGCCATTATAGAAGGTCTTTCTAGCATCTTTGAAAGATCCAGATCTTACCCGGAAACGTTGGTGCACGACGTGGTGCTCGAATCTGATGCCGCCGAGGTCGTGAAGCTGTTTAATGGAGAAGTCGATGATCTATCGGATATCTCCTTCCTGATCGACGAAGCCCGTGAGATCGCGTCCAAGCTGTCTTTGGTCTCCTTCGTCCATTGCCCAAGGGAGTCGAATTTTTTGGCGCACTATCTGGCACGCAAAGTTGTTAGGAATTTTGGTTTTGTGCATTTCGTTTTTGATCCTTCTCTTTGCTCGAGAATGGATAGCCCTGCTGGTGAAGGGAATGTTTTACCCCTTTGGGCTACCTCCCTGTTGCGGGAGGGAGGGCTTCTTCGGCGCCTTCTTCTTCAATTTTTGGACGGCAGCGCGGCGTCCTCCAGCGACGGCAGGCAACGACGACAACTACTTCCGGCGGTAGCAGAACAGTCCGACGACGCGCGACACTTCCAGCCGAGTCTCCCTCTCTCTCTCTCTCGCGTCGACTGGTGCAGCGAAGGCCTGCGTTCGTCCTTCCGGTGCGTGGCGGCGTCGCGTGGGGAGCAGCAGCGAGCGGCGATTTCGTTTGTGGTTGTCTCTTCCGGCCGTGCAGTGTGTAGCAGTGATTTCACACGACCGGCAGCGTTCTCGGCGTGGGTGCATAGTGGGTCGGCGACCAGCAACTTCTTTCGACGATTTGAGGTAAAGGCAAGGGCAGATGAAAGGCGATTGATGAGAATCCGTTAG

Coding sequence (CDS)

ATGTGGGACTTTGGTCACACTCCCAACACTCCATGTCCTGGGTGTCCACCTCAGTTTCTACTCCCTGATCACTACAGGATCCCTTCCGGCAAACAAATGGTTGCCCATTTCCGTCCATGCATGGTTGGTGCTCCTCCTAGAGAGAACCCGGAACCAGGACCAGGAGCATGTGTTCTGGAATTGGAATGGTGGAAATTCAAGGACTTTTGGTCCTGTGCTTTGAATCTGCTGGAGAAGCATGAAGCAGGGGTGGCTGGACACATTATTTGGGAGCTCTGGACCATGAGGAACAAATTGAAGCAGACCAACAACAGGCAAAATCTCACACAAATCTCAGAGGATATTTTCTATGCTATTGACGAATACATCTCAGAGATGGAGAATTCTAACCAGCACAGGGAGTCGGAGAACCTTCTGAGTCCCGATGTTTGGAAGCCGCCGGTTGACCTTCAGTGGAAGATGAACGTTGATGCGGCCTGGTTCGAAGCCTCGAATTCAGGGGGCCTGGGGTGGATCATTCGAGACTCAGTAGGATCTCTGATAGGAGCTGGAAGCAAGAAGATTGATAGATTTTCGGAGATTAAAATCTTGGAAGCCATGGCCATTATAGAAGGTCTTTCTAGCATCTTTGAAAGATCCAGATCTTACCCGGAAACGTTGGTGCACGACGTGGTGCTCGAATCTGATGCCGCCGAGGTCGTGAAGCTGTTTAATGGAGAAGTCGATGATCTATCGGATATCTCCTTCCTGATCGACGAAGCCCGTGAGATCGCGTCCAAGCTGTCTTTGGTCTCCTTCGTCCATTGCCCAAGGGAGTCGAATTTTTTGGCGCACTATCTGGCACGCAAAGTTGTTAGGAATTTTGGTTTTGTGCATTTCGTTTTTGATCCTTCTCTTTGCTCGAGAATGGATAGCCCTGCTGGTGAAGGGAATGTTTTACCCCTTTGGGCTACCTCCCTGTTGCGGGAGGGAGGGCTTCTTCGGCGCCTTCTTCTTCAATTTTTGGACGGCAGCGCGGCGTCCTCCAGCGACGGCAGGCAACGACGACAACTACTTCCGGCGGTAGCAGAACAGTCCGACGACGCGCGACACTTCCAGCCGAGTCTCCCTCTCTCTCTCTCTCGCGTCGACTGGTGCAGCGAAGGCCTGCGTTCGTCCTTCCGGTGCGTGGCGGCGTCGCGTGGGGAGCAGCAGCGAGCGGCGATTTCGTTTGTGGTTGTCTCTTCCGGCCGTGCAGTGTGTAGCAGTGATTTCACACGACCGGCAGCGTTCTCGGCGTGGGTGCATAGTGGGTCGGCGACCAGCAACTTCTTTCGACGATTTGAGGTAAAGGCAAGGGCAGATGAAAGGCGATTGATGAGAATCCGTTAG

Protein sequence

MWDFGHTPNTPCPGCPPQFLLPDHYRIPSGKQMVAHFRPCMVGAPPRENPEPGPGACVLELEWWKFKDFWSCALNLLEKHEAGVAGHIIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDEYISEMENSNQHRESENLLSPDVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIKILEAMAIIEGLSSIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAREIASKLSLVSFVHCPRESNFLAHYLARKVVRNFGFVHFVFDPSLCSRMDSPAGEGNVLPLWATSLLREGGLLRRLLLQFLDGSAASSSDGRQRRQLLPAVAEQSDDARHFQPSLPLSLSRVDWCSEGLRSSFRCVAASRGEQQRAAISFVVVSSGRAVCSSDFTRPAAFSAWVHSGSATSNFFRRFEVKARADERRLMRIR

Homology

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BLAST of Spg020777 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 116.7 bits (291), Expect = 5.2e-22
Identity = 74/213 (34.74%), Postives = 111/213 (52.11%), Query Frame = 0


Query: 88  IIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDEYI--SEMENSNQHRESENL------- 147
           I W++W MRNK    +  + +   + DI   ID YI  S   ++N   +S N        
Sbjct: 15  IAWQIWEMRNK----SIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDLHLIRR 74

Query: 148 ---LSPDVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIK 207
               +   WKPP    WK+N DAAW   +N+GG+GWI+RD  G +I A  + I     I 
Sbjct: 75  IGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNIT 134

Query: 208 ILEAMAIIEGLSSI-FERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEA 267
            LE MAI EGL +I  E  R   +     + LESD+ E + L + +  D ++I +L++E 
Sbjct: 135 YLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEI 194

Query: 268 REIASKLSLVSFVHCPRESNFLAHYLARKVVRN 288
            ++   + +VS  H  RE+N +AH LAR+ + N
Sbjct: 195 WQMMEDMKIVSMRHISREANKVAHDLARRAMEN 223

[Close]

BLAST of Spg020777 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 114.8 bits (286), Expect = 2.0e-21
Identity = 73/212 (34.43%), Postives = 110/212 (51.89%), Query Frame = 0


Query: 88  IIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDEYI--SEMENSNQHRESENL------- 147
           I W++W MRNK    +  + +   + DI  AID YI  S   N+N   +S N        
Sbjct: 15  IAWQIWEMRNK----SIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKSTNKDLHLIRR 74

Query: 148 ---LSPDVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIK 207
               +   WKPP    WK+N +AAW   +N+GG+GWI+RD  G +I A  + I     I 
Sbjct: 75  IEDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNIT 134

Query: 208 ILEAMAIIEGLSSIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAR 267
            LE MAI EGL +I +            + LESD+ E + L + +  D ++I +L++E  
Sbjct: 135 YLEVMAICEGLRAIRQEH-------CRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIW 194

Query: 268 EIASKLSLVSFVHCPRESNFLAHYLARKVVRN 288
           ++   + +VS  H  RE+N +AH LAR+ + N
Sbjct: 195 QMMKDMEIVSMRHISREANKVAHGLARRAMEN 215

[Close]

BLAST of Spg020777 vs. NCBI nr
Match: XP_030969743.1 (uncharacterized protein LOC115990020 [Quercus lobata])

HSP 1 Score: 104.0 bits (258), Expect = 3.5e-18
Identity = 69/230 (30.00%), Postives = 104/230 (45.22%), Query Frame = 0


Query: 56   ACVLELEWWKFKDFWSCALNLLEKHEAGVAGHIIWELWTMRNKLKQTNNRQNLTQISEDI 115
            A + E     F D +   L L           I W LW  RN L++      L  +S   
Sbjct: 1293 AALYEKHHGSFMDLFESVLKLNSSFNVAWFATIAWSLWKRRNNLRECQPTWPLLDVSRRA 1352

Query: 116  FYAIDEYISEMENSNQHRESENLLSPDVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRD 175
               ++E+    E  N+ R+ + +  P  WKPP +  +K N DAA FE     GLG ++RD
Sbjct: 1353 CNLVEEF---FEVQNRTRKPDIIPPPVKWKPPPEPVYKANFDAALFEQLGLAGLGVVLRD 1412

Query: 176  SVGSLIGAGSKKIDRFSEIKILEAMAIIEGLSSIFERSRSYPETLVHDVVLESDAAEVVK 235
            S G +I A S+KI     +++ EA+A    +    E S       +  V++E D   V++
Sbjct: 1413 SGGHIIAALSEKIKLPQTVELAEALAARRAVLFATELS-------IFKVMVEGDCLRVIQ 1472

Query: 236  LFNGEVDDLSDISFLIDEAREIASKLSLVSFVHCPRESNFLAHYLARKVV 286
                +   L+    +++E R + S L L SF H  RE N LAH L  + V
Sbjct: 1473 ALKEQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKLAHSLVHRAV 1512

[Close]

BLAST of Spg020777 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 102.8 bits (255), Expect = 7.8e-18
Identity = 57/196 (29.08%), Postives = 105/196 (53.57%), Query Frame = 0


Query: 90  WELWTMRNKLKQTNNRQNLTQISEDI--FYAIDEYISEMENSNQHRESENLLSPDVWKPP 149
           W +W  RN +       + + + + +  F     Y SE   S  H+   N L    W+PP
Sbjct: 18  WVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQSETSLSMLHKTLNNKLK---WEPP 77

Query: 150 VDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIKILEAMAIIEGLS 209
               W +N DA+W ++++ GG+GWIIR   G ++ AG++ ++  + +K+LEA AI+EGL 
Sbjct: 78  PMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLR 137

Query: 210 SIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAREIASKLSLVSFV 269
           ++          ++  + +E+D+AEV  L N + +DL+   ++++E   +     +++F 
Sbjct: 138 NLTNLG------VLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFA 197

Query: 270 HCPRESNFLAHYLARK 284
              RE+N  AH LA++
Sbjct: 198 KVGRETNGCAHSLAQR 204

[Close]

BLAST of Spg020777 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 99.4 bits (246), Expect = 8.7e-17
Identity = 66/195 (33.85%), Postives = 93/195 (47.69%), Query Frame = 0


Query: 64  WKFKDFWSCALNLLEKHEAGVAGHIIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDE-- 123
           W  KD W+  +N+L   E   +  I W++W  RN+        +  Q+   I   I+   
Sbjct: 13  WTNKDSWNWLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDEQQLDRSIVLFINSNI 72

Query: 124 ----YISEMENSNQH-------RESENLLSPDVWKPPVDLQWKMNVDAAWFEASNSGGLG 183
                IS+   S Q+       RE+ N+L    W  P    WK+N DA+W E    GG+G
Sbjct: 73  DKGTCISQTRRSQQNDGYLPRGRENLNMLGVR-WSAPPTNCWKLNTDASWSEEREVGGIG 132

Query: 184 WIIRDSVGSLIGAGSKKIDRFSEIKILEAMAIIEGLSSIFERSRSYPETLVHDVVLESDA 243
           WI+ D  G ++ AG+ KI    EI  LE M II GL  I  +SRS        + LESD+
Sbjct: 133 WILCDCRGEIVLAGNCKIREKKEINALELMTIIRGLQFINMQSRS-------PIYLESDS 192

Query: 244 AEVVKLFNGEVDDLS 246
            EV++L   E  DL+
Sbjct: 193 VEVIRLMKKEDVDLT 199

[Close]

BLAST of Spg020777 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.5e-22
Identity = 74/213 (34.74%), Postives = 111/213 (52.11%), Query Frame = 0


Query: 88  IIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDEYI--SEMENSNQHRESENL------- 147
           I W++W MRNK    +  + +   + DI   ID YI  S   ++N   +S N        
Sbjct: 15  IAWQIWEMRNK----SIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDLHLIRR 74

Query: 148 ---LSPDVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIK 207
               +   WKPP    WK+N DAAW   +N+GG+GWI+RD  G +I A  + I     I 
Sbjct: 75  IGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNIT 134

Query: 208 ILEAMAIIEGLSSI-FERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEA 267
            LE MAI EGL +I  E  R   +     + LESD+ E + L + +  D ++I +L++E 
Sbjct: 135 YLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEI 194

Query: 268 REIASKLSLVSFVHCPRESNFLAHYLARKVVRN 288
            ++   + +VS  H  RE+N +AH LAR+ + N
Sbjct: 195 WQMMEDMKIVSMRHISREANKVAHDLARRAMEN 223

[Close]

BLAST of Spg020777 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 9.7e-22
Identity = 73/212 (34.43%), Postives = 110/212 (51.89%), Query Frame = 0


Query: 88  IIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDEYI--SEMENSNQHRESENL------- 147
           I W++W MRNK    +  + +   + DI  AID YI  S   N+N   +S N        
Sbjct: 15  IAWQIWEMRNK----SIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKSTNKDLHLIRR 74

Query: 148 ---LSPDVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIK 207
               +   WKPP    WK+N +AAW   +N+GG+GWI+RD  G +I A  + I     I 
Sbjct: 75  IEDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNIT 134

Query: 208 ILEAMAIIEGLSSIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAR 267
            LE MAI EGL +I +            + LESD+ E + L + +  D ++I +L++E  
Sbjct: 135 YLEVMAICEGLRAIRQEH-------CRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIW 194

Query: 268 EIASKLSLVSFVHCPRESNFLAHYLARKVVRN 288
           ++   + +VS  H  RE+N +AH LAR+ + N
Sbjct: 195 QMMKDMEIVSMRHISREANKVAHGLARRAMEN 215

[Close]

BLAST of Spg020777 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 3.8e-18
Identity = 57/196 (29.08%), Postives = 105/196 (53.57%), Query Frame = 0


Query: 90  WELWTMRNKLKQTNNRQNLTQISEDI--FYAIDEYISEMENSNQHRESENLLSPDVWKPP 149
           W +W  RN +       + + + + +  F     Y SE   S  H+   N L    W+PP
Sbjct: 18  WVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQSETSLSMLHKTLNNKLK---WEPP 77

Query: 150 VDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIKILEAMAIIEGLS 209
               W +N DA+W ++++ GG+GWIIR   G ++ AG++ ++  + +K+LEA AI+EGL 
Sbjct: 78  PMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLR 137

Query: 210 SIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAREIASKLSLVSFV 269
           ++          ++  + +E+D+AEV  L N + +DL+   ++++E   +     +++F 
Sbjct: 138 NLTNLG------VLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFA 197

Query: 270 HCPRESNFLAHYLARK 284
              RE+N  AH LA++
Sbjct: 198 KVGRETNGCAHSLAQR 204

[Close]

BLAST of Spg020777 vs. ExPASy TrEMBL
Match: A0A2N9EZZ2 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS12168 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 8.5e-18
Identity = 65/218 (29.82%), Postives = 106/218 (48.62%), Query Frame = 0


Query: 66  FKDFWSCALNLLEKHEAGVAGHIIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDEYISE 125
           F D  S  L++    +      I W LW  RNKL+     + + Q+       ++EY  E
Sbjct: 236 FADLLSKVLHVGRSSDPETFIVICWALWQRRNKLRIHQEVEPINQVGSKAKCYLEEYNRE 295

Query: 126 MENSNQHRESENLLSPDVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGS 185
            ++SN + ++        W PP  L++K+N D A F+ +N  G+G I+RDS G ++ + +
Sbjct: 296 NDHSNPNPQAAPATR---WMPPKMLRYKINYDGAVFKETNEAGIGVIVRDSQGLVMASLT 355

Query: 186 KKIDRFSEIKILEAMAIIEGLSSIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLS 245
           +K+     +  +EA A+   +    E         + +   E D+  +V   N +   L+
Sbjct: 356 QKVLFPLSVPSIEAWAVKRSIQFALEIG-------ITEAEFEGDSQTIVNALNAQHPSLA 415

Query: 246 DISFLIDEAREIASKLSLVSFVHCPRESNFLAHYLARK 284
               L+ +A+E+ASKL   SF H  RE N LAH LARK
Sbjct: 416 PFGLLLADAKELASKLQNFSFSHVKREGNRLAHALARK 443

[Close]

BLAST of Spg020777 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.2e-17
Identity = 66/195 (33.85%), Postives = 93/195 (47.69%), Query Frame = 0


Query: 64  WKFKDFWSCALNLLEKHEAGVAGHIIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDE-- 123
           W  KD W+  +N+L   E   +  I W++W  RN+        +  Q+   I   I+   
Sbjct: 13  WTNKDSWNWLVNMLSDEEVATSMVIAWQIWESRNRSIFRGETIDEQQLDRSIVLFINSNI 72

Query: 124 ----YISEMENSNQH-------RESENLLSPDVWKPPVDLQWKMNVDAAWFEASNSGGLG 183
                IS+   S Q+       RE+ N+L    W  P    WK+N DA+W E    GG+G
Sbjct: 73  DKGTCISQTRRSQQNDGYLPRGRENLNMLGVR-WSAPPTNCWKLNTDASWSEEREVGGIG 132

Query: 184 WIIRDSVGSLIGAGSKKIDRFSEIKILEAMAIIEGLSSIFERSRSYPETLVHDVVLESDA 243
           WI+ D  G ++ AG+ KI    EI  LE M II GL  I  +SRS        + LESD+
Sbjct: 133 WILCDCRGEIVLAGNCKIREKKEINALELMTIIRGLQFINMQSRS-------PIYLESDS 192

Query: 244 AEVVKLFNGEVDDLS 246
            EV++L   E  DL+
Sbjct: 193 VEVIRLMKKEDVDLT 199

[Close]

BLAST of Spg020777 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-07
Identity = 43/204 (21.08%), Postives = 81/204 (39.71%), Query Frame = 0


Query: 88  IIWELWTMRNKLKQTNNRQNLTQISEDIFYAIDEYI-----SEMENSNQHRESENLLSPD 147
           ++W +W   N L   + R       E       E++     +E +N N++ +        
Sbjct: 47  LMWRIWKSGNDLVFNHTRTKFQTTVEMALNDTKEWLDNTMTNEQQNGNRNADPSRNTK-- 106

Query: 148 VWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIKILEAMAI 207
            W PP   + K N DA+  E +   GLGWI+R+S G++I  G  K       +  E   +
Sbjct: 107 -WSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTL 166

Query: 208 IEGLSSIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAREIASKLS 267
           I  + + +             V+ E D   + ++ N +  +   +   +D  +       
Sbjct: 167 IWAIQASYGFGHK-------KVIFEGDNQTITRMINTKSSN-PRLQHFLDTIQSWIPSFE 226

Query: 268 LVSFVHCPRESNFLAHYLARKVVR 287
            + F    RE N  A +LA++ ++
Sbjct: 227 SIEFSFKHREQNGCADFLAKQAIK 239

[Close]

BLAST of Spg020777 vs. TAIR 10
Match: AT2G13980.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 2.8e-05
Identity = 40/139 (28.78%), Postives = 59/139 (42.45%), Query Frame = 0


Query: 144 WKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIKILEAMAII 203
           WK P D   K N DA +   +      WIIR+  G     GS ++D  S     E  A++
Sbjct: 13  WKAPPDTHIKCNYDAGFNVQNLDSTARWIIRNHDGIAQHWGSLQLDNTSTPLEAETKALL 72

Query: 204 EGLSSIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAREIASKLSL 263
             L   + R           V++E D   +  L +G     + ++ L+D+ R  A K S 
Sbjct: 73  AALQQTWIRG-------YLRVIMEGDCETLTNLVSGS-SSHNHLANLLDDIRIWAKKFSN 132

Query: 264 VSFVHCPRESNFLAHYLAR 283
           V F    R  N +AH LA+
Sbjct: 133 VQFSFVRRGGNKVAHELAK 143

[Close]

BLAST of Spg020777 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 45.4 bits (106), Expect = 1.4e-04
Identity = 34/135 (25.19%), Postives = 61/135 (45.19%), Query Frame = 0


Query: 153 KMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIKILEAMAIIEGLSSIFER 212
           K N DA+  E     GLGW+IR+S G+++  G  K       +  E  A+I  +    + 
Sbjct: 49  KCNYDASHHEGDVVSGLGWLIRNSQGTVLECGMGKFQGRMTPEEAECSALIWAI----QA 108

Query: 213 SRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAREIASKLSLVSFVHCPRE 272
           + ++  T    V+ E D + V +L N + D+   +   +D  +      +   F+   RE
Sbjct: 109 TSAFGYT---KVIFEGDNSNVNRLINTKSDN-PRLKHYLDTIKSWIPSFTSTEFIFTHRE 168

Query: 273 SNFLAHYLARKVVRN 288
            N  A  L +K +++
Sbjct: 169 QNQCADTLVKKAIKS 175

[Close]

BLAST of Spg020777 vs. TAIR 10
Match: AT3G23320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 5.3e-04
Identity = 36/146 (24.66%), Postives = 57/146 (39.04%), Query Frame = 0


Query: 142 DVWKPPVDLQWKMNVDAAWFEASNSGGLGWIIRDSVGSLIGAGSKKIDRFSEIKILEAMA 201
           D W+ P     K N D +        GL WIIR+S G+ +  G  K      IK  E  A
Sbjct: 28  DKWQKPGAEWVKCNYDVSNHAGRQDSGLRWIIRNSQGTCLDCGCGKFQGRQTIKEAECTA 87

Query: 202 IIEGLSSIFERSRSYPETLVHDVVLESDAAEVVKLFNGEVDDLSDISFLIDEAREIASKL 261
           +I  +   ++            V  E D   V +L   + +    + + ++  ++ +   
Sbjct: 88  LIWAIQCAWDLG-------YRRVEFEGDNITVNRLIRNK-ETNPRLRYYLEFIQQWSKAF 147

Query: 262 SLVSFVHCPRESNFLAHYLARKVVRN 288
           + V F    RE N     LA+K V N
Sbjct: 148 TTVKFTFRHREQNVCVDVLAKKAVAN 165

The following BLAST results are available for this feature:

BLAST of Spg020777 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156777.1	5.2e-22	34.74	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143535.1	2.0e-21	34.43	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_030969743.1	3.5e-18	30.00	uncharacterized protein LOC115990020 [Quercus lobata]	[more]
XP_022155262.1	7.8e-18	29.08	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022143317.1	8.7e-17	33.85	uncharacterized protein LOC111013216 [Momordica charantia]	[more]

BLAST of Spg020777 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg020777 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DSV1	2.5e-22	34.74	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CP26	9.7e-22	34.43	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DNV9	3.8e-18	29.08	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A2N9EZZ2	8.5e-18	29.82	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS12168 PE=4 SV=1	[more]
A0A6J1CQG0	4.2e-17	33.85	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]

BLAST of Spg020777 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65005.1	1.3e-07	21.08	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G13980.1	2.8e-05	28.78	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G52990.1	1.4e-04	25.19	thioredoxin family protein	[more]
AT3G23320.1	5.3e-04	24.66	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 150..286 e-value: 4.7E-13 score: 51.3
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 155..283 e-value: 3.1E-21 score: 75.4
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 142..283
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 142..283
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 155..282 e-value: 1.45044E-22 score: 90.4512
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 152..288

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg020777.1	Spg020777.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity