Spg020674 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg020674
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Chitotriosidase-1-like
Location	scaffold9: 15691036 .. 15692085 (-)
RNA-Seq Expression	Spg020674
Synteny	Spg020674

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGGCGTTTACTGGTTATGCGGCTCCGATGTTTCAGTGGCCGACATAGAATCTTCTCTTTTCACCCATATTTTCTGTGCCTTCGCCAATCTCGATAAAACCACAAACAAAGTCACAATTCCTTCTCAATTCTCCGCCCAATTCTCAACCTTCACTGAAACCGTTCGACGAAAAAATCCCGATGTGAAAACACTCTTGTCCATCGGCGGAGGCAACGCCACAGCTGACGATTTCGCTTCAATGGCCAGCCAGCCCAATTCCCGCCAATCCTTCATCCATTCGTCCATTTCCCTGGCCCGATCCTACAATTTCCACGGCCTCGATCTCGATTGGGAATACCCAAAAACCGAAAGTCAAATGGCCGCGTTCTCCACTCTTCTCACTGAGTGGCGCGCCGCCGTGGAGAGAGAATCCCGATCCTCCCGCATGCCCCAGCTTCTTCTCTCCGCCGCGGTTTTTCGGTCCTCTGACTATTACAGAGTTCCAATCCCCACTTCATCTCTAGCCCTAAATTTGGATTGGATCAATGTTATGACTTATGATTTTTATGGCCCCCTTTGGTCTGCGGACTCGACGGGGGCGCCGGCAGCGCTGTACAGATCCCCCATGCAATGGCAAGTGAACTGTGACGAGGCTATAATGTCGTGGATTCATTCGGGTTTTCCCGCAAGTAAAATTGTGTTGGGGATTCCGTTTTACGGGTGGGCGTGGCAGCTGCAAAATCCCAATCCTAACAATAATGGGTTGTATGCTCCGGCAAATGGACCGGCAGCCATACCTGAAACTGGCAACGACGGATCGATAACTTATAAACAAATAAAGAGGTCATTTATAGAGACGAACAGAGTAAATCCAATGTACGATAGCGAGATTGTTTCCAATTATGTGCACTCGGGAACGACTTGGATTGGGTATGATGATACGCAGAGTGTTGCGGCAAAGGTTTCTTATGCTAAGAGGATGGAATTGTTTGGTTATTTTGCTTGGCAGGTTGCTGCCGATGATGATACCTGGACTCTGTCTCGAACCGGTACTTATTCTCGATAA

mRNA sequence

ATGAAGGGCGTTTACTGGTTATGCGGCTCCGATGTTTCAGTGGCCGACATAGAATCTTCTCTTTTCACCCATATTTTCTGTGCCTTCGCCAATCTCGATAAAACCACAAACAAAGTCACAATTCCTTCTCAATTCTCCGCCCAATTCTCAACCTTCACTGAAACCGTTCGACGAAAAAATCCCGATGTGAAAACACTCTTGTCCATCGGCGGAGGCAACGCCACAGCTGACGATTTCGCTTCAATGGCCAGCCAGCCCAATTCCCGCCAATCCTTCATCCATTCGTCCATTTCCCTGGCCCGATCCTACAATTTCCACGGCCTCGATCTCGATTGGGAATACCCAAAAACCGAAAGTCAAATGGCCGCGTTCTCCACTCTTCTCACTGAGTGGCGCGCCGCCGTGGAGAGAGAATCCCGATCCTCCCGCATGCCCCAGCTTCTTCTCTCCGCCGCGGTTTTTCGGTCCTCTGACTATTACAGAGTTCCAATCCCCACTTCATCTCTAGCCCTAAATTTGGATTGGATCAATGTTATGACTTATGATTTTTATGGCCCCCTTTGGTCTGCGGACTCGACGGGGGCGCCGGCAGCGCTGTACAGATCCCCCATGCAATGGCAAGTGAACTGTGACGAGGCTATAATGTCGTGGATTCATTCGGGTTTTCCCGCAAGTAAAATTGTGTTGGGGATTCCGTTTTACGGGTGGGCGTGGCAGCTGCAAAATCCCAATCCTAACAATAATGGGTTGTATGCTCCGGCAAATGGACCGGCAGCCATACCTGAAACTGGCAACGACGGATCGATAACTTATAAACAAATAAAGAGGTCATTTATAGAGACGAACAGAGTAAATCCAATGTACGATAGCGAGATTGTTTCCAATTATGTGCACTCGGGAACGACTTGGATTGGGTATGATGATACGCAGAGTGTTGCGGCAAAGGTTTCTTATGCTAAGAGGATGGAATTGTTTGGTTATTTTGCTTGGCAGGTTGCTGCCGATGATGATACCTGGACTCTGTCTCGAACCGGTACTTATTCTCGATAA

Coding sequence (CDS)

ATGAAGGGCGTTTACTGGTTATGCGGCTCCGATGTTTCAGTGGCCGACATAGAATCTTCTCTTTTCACCCATATTTTCTGTGCCTTCGCCAATCTCGATAAAACCACAAACAAAGTCACAATTCCTTCTCAATTCTCCGCCCAATTCTCAACCTTCACTGAAACCGTTCGACGAAAAAATCCCGATGTGAAAACACTCTTGTCCATCGGCGGAGGCAACGCCACAGCTGACGATTTCGCTTCAATGGCCAGCCAGCCCAATTCCCGCCAATCCTTCATCCATTCGTCCATTTCCCTGGCCCGATCCTACAATTTCCACGGCCTCGATCTCGATTGGGAATACCCAAAAACCGAAAGTCAAATGGCCGCGTTCTCCACTCTTCTCACTGAGTGGCGCGCCGCCGTGGAGAGAGAATCCCGATCCTCCCGCATGCCCCAGCTTCTTCTCTCCGCCGCGGTTTTTCGGTCCTCTGACTATTACAGAGTTCCAATCCCCACTTCATCTCTAGCCCTAAATTTGGATTGGATCAATGTTATGACTTATGATTTTTATGGCCCCCTTTGGTCTGCGGACTCGACGGGGGCGCCGGCAGCGCTGTACAGATCCCCCATGCAATGGCAAGTGAACTGTGACGAGGCTATAATGTCGTGGATTCATTCGGGTTTTCCCGCAAGTAAAATTGTGTTGGGGATTCCGTTTTACGGGTGGGCGTGGCAGCTGCAAAATCCCAATCCTAACAATAATGGGTTGTATGCTCCGGCAAATGGACCGGCAGCCATACCTGAAACTGGCAACGACGGATCGATAACTTATAAACAAATAAAGAGGTCATTTATAGAGACGAACAGAGTAAATCCAATGTACGATAGCGAGATTGTTTCCAATTATGTGCACTCGGGAACGACTTGGATTGGGTATGATGATACGCAGAGTGTTGCGGCAAAGGTTTCTTATGCTAAGAGGATGGAATTGTTTGGTTATTTTGCTTGGCAGGTTGCTGCCGATGATGATACCTGGACTCTGTCTCGAACCGGTACTTATTCTCGATAA

Protein sequence

MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKNPDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQMAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMTYDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQLQNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSGTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGTYSR

Homology

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BLAST of Spg020674 vs. NCBI nr
Match: XP_038879961.1 (class V chitinase-like [Benincasa hispida])

HSP 1 Score: 459.9 bits (1182), Expect = 1.9e-125
Identity = 238/346 (68.79%), Postives = 275/346 (79.48%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           MKG+YW  GS   V+DI+SSLFTHIFCAFA+L   T +VTI    SA+F TFT+TV+RKN
Sbjct: 28  MKGIYWFSGSGFPVSDIDSSLFTHIFCAFADLAPNTFQVTISPSNSAKFKTFTQTVQRKN 87

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P+VKTLLSIGGG A AD FA+MASQP+SR+SFI SSISLARS  F GLDLDWEYP  ++Q
Sbjct: 88  PNVKTLLSIGGGGANADTFAAMASQPDSRKSFISSSISLARSNGFLGLDLDWEYPSNQNQ 147

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           +++FSTL TEWRAA E+ES+SS  P+LLLSAAVFRSS+YY +P+P S LA  LDWINVM 
Sbjct: 148 ISSFSTLCTEWRAAAEKESQSSGKPRLLLSAAVFRSSNYYGLPLPASVLATKLDWINVMC 207

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS +ST  PAAL+ S    QVNCD  I SWI SGFPASKIVLGIPFYGWAW+L
Sbjct: 208 YDFYGPGWSPNSTAPPAALFGS--AGQVNCDAGITSWIQSGFPASKIVLGIPFYGWAWRL 267

Query: 241 QNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSG 300
              N NNNGLYAPANG AA     +DG+ITYK I  +F E NRVN +Y+  +V+NYV SG
Sbjct: 268 --VNQNNNGLYAPANG-AATGAGIDDGAITYKGI-NAFKENNRVNSVYNGMVVTNYVSSG 327

Query: 301 TTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           TTWIGYDD QSVAAKV+YAK+  LFGYFAWQV ADD+ WTLSRT +
Sbjct: 328 TTWIGYDDKQSVAAKVAYAKKKGLFGYFAWQVGADDN-WTLSRTAS 366

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BLAST of Spg020674 vs. NCBI nr
Match: XP_023529986.1 (chitotriosidase-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438.0 bits (1125), Expect = 7.9e-119
Identity = 222/345 (64.35%), Postives = 274/345 (79.42%), Query Frame = 0


Query: 2   KGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKNP 61
           KGVYW  GS+  VA+I S+LFTHIFCAFA+L+  TN+VTI S  +A+FSTFT+TVR+KNP
Sbjct: 33  KGVYWFSGSEFPVANINSALFTHIFCAFADLNTNTNQVTISSSNAAKFSTFTQTVRQKNP 92

Query: 62  DVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQM 121
           +VKT+LSIGGG++   +FASMASQ  SR+SFI SSI+LAR+  F GLDLDWEYP T ++M
Sbjct: 93  NVKTMLSIGGGSSNPTNFASMASQSGSRKSFIDSSITLARNNGFDGLDLDWEYPSTPTEM 152

Query: 122 AAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMTY 181
            +F+TLLTEWRAAV +ES SS  P+LLLSAAVFRSS+YY  P+P SS++ +LDWINVM+Y
Sbjct: 153 NSFATLLTEWRAAVVKESVSSGKPRLLLSAAVFRSSNYYGTPLPVSSISNSLDWINVMSY 212

Query: 182 DFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQLQ 241
           DFYGP WSA ST  PAAL+   +  QVN +  I  WI SG PA+KI +G PFYGWAW+L 
Sbjct: 213 DFYGPGWSASSTAPPAALF--GLGGQVNGNAGIREWIQSGMPANKIAIGFPFYGWAWRL- 272

Query: 242 NPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSGT 301
             + N NGLYAPANG AA     + G+ITY+ IK +FI TNRV P+Y++ +VSNYV+SGT
Sbjct: 273 -VDQNKNGLYAPANG-AATGAGISGGAITYRDIK-TFIATNRVTPVYNATVVSNYVYSGT 332

Query: 302 TWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           TWIGYDD QS+AAKVSYA    L+GYFAWQ+AADDD++TLSRT +
Sbjct: 333 TWIGYDDKQSIAAKVSYANANGLWGYFAWQIAADDDSFTLSRTAS 371

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BLAST of Spg020674 vs. NCBI nr
Match: KAG6587842.1 (Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 438.0 bits (1125), Expect = 7.9e-119
Identity = 223/345 (64.64%), Postives = 272/345 (78.84%), Query Frame = 0


Query: 2   KGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKNP 61
           KGVYW  GS+  VA+I S+LFTHIFCAFA+L+  TN+VTI S  +A+FSTFT+TVR+KNP
Sbjct: 32  KGVYWFSGSEFPVANINSALFTHIFCAFADLNTNTNQVTISSSNAAKFSTFTQTVRQKNP 91

Query: 62  DVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQM 121
           +VKTLLSIGGG++   +FASMASQ  SR+SFI SSI+LAR+  F GLDLDWEYP T ++M
Sbjct: 92  NVKTLLSIGGGSSNPTNFASMASQSGSRKSFIDSSITLARNNGFDGLDLDWEYPSTPTEM 151

Query: 122 AAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMTY 181
            +F TLLTEWRAAV +ES SS  P+LLLSAAVFRSS+YY  P+P SS++ +LDWINVM+Y
Sbjct: 152 NSFGTLLTEWRAAVVKESVSSGKPRLLLSAAVFRSSNYYGTPLPVSSISNSLDWINVMSY 211

Query: 182 DFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQLQ 241
           DFYGP WSA ST  PAAL+ S    QVN +  I  WI SG PA+KI +G PFYGWAW+L 
Sbjct: 212 DFYGPGWSASSTAPPAALFGS--GGQVNGNAGIREWIQSGMPANKIAIGFPFYGWAWRL- 271

Query: 242 NPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSGT 301
             + N NG YAPANG AA     + G+ITY+ IK +FI TNRV P+Y++ +VSNYV+SGT
Sbjct: 272 -VDQNKNGFYAPANG-AATGAGISGGAITYRDIK-TFIATNRVTPVYNATVVSNYVYSGT 331

Query: 302 TWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           TWIGYDD QS+AAKVSYA    L+GYFAWQ+AADDD++TLSRT +
Sbjct: 332 TWIGYDDKQSIAAKVSYANANGLWGYFAWQIAADDDSFTLSRTAS 370

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BLAST of Spg020674 vs. NCBI nr
Match: KAA0035051.1 (chitotriosidase-1-like [Cucumis melo var. makuwa])

HSP 1 Score: 436.4 bits (1121), Expect = 2.3e-118
Identity = 225/347 (64.84%), Postives = 268/347 (77.23%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           MKG+YW  GS+  ++DI+SSLFTHI+CAFA+L+  TN+VTI S  S  F +FT  +RRKN
Sbjct: 32  MKGIYWFSGSEFPLSDIDSSLFTHIYCAFADLNPNTNQVTISSSNSESFKSFTPILRRKN 91

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P+VKTLLSIGGG A A+ FASMASQPNSR+SFI SSISLARSYN  GLD+DWEYP  ++Q
Sbjct: 92  PNVKTLLSIGGGGADANAFASMASQPNSRKSFIDSSISLARSYNLSGLDIDWEYPSNQNQ 151

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           + +F TL +EWRAAVE ES+S+  P+L LSAAVFRSS+YY  P+P S LA  LDWINVM 
Sbjct: 152 INSFKTLCSEWRAAVENESQSTGKPRLFLSAAVFRSSNYYGTPLPASDLATKLDWINVMC 211

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS + T  PAALY S    QVNC+  I SWI SGFPA+KIV+G+PFYGWAW+L
Sbjct: 212 YDFYGPGWSPNFTAPPAALYGS--SGQVNCNTGITSWIQSGFPANKIVIGMPFYGWAWRL 271

Query: 241 QNPNPNNNGLYAPANGPAAIPETGND-GSITYKQIKRSFIETNRVNPMYDSEIVSNYVHS 300
              + + NGLYAPANG A    TG D G+ITYK I   F + N VN +Y+  +V+NYV S
Sbjct: 272 --VSQSKNGLYAPANGAAT--GTGIDGGAITYKGI-NEFKKRNGVNGVYNGTVVTNYVRS 331

Query: 301 GTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           GTTWIGYDD +SVAAKV YAK+  LFGYFAWQVAADD+ +TLSR GT
Sbjct: 332 GTTWIGYDDKESVAAKVGYAKKKGLFGYFAWQVAADDN-FTLSRLGT 370

[Close]

BLAST of Spg020674 vs. NCBI nr
Match: XP_022973419.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 435.6 bits (1119), Expect = 3.9e-118
Identity = 220/345 (63.77%), Postives = 271/345 (78.55%), Query Frame = 0


Query: 2   KGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKNP 61
           KGVYW  GS+  VA+I S+LFTHIFCAFA+L+  TN+VTI S  +A+FSTFT+TVR+KNP
Sbjct: 33  KGVYWFSGSEFPVANINSALFTHIFCAFADLNTNTNQVTISSSNAAKFSTFTQTVRQKNP 92

Query: 62  DVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQM 121
           +VKT+LSIGGG++   +FASMASQPNSR+SF+ SSI+LAR+  F GLDLDWEYP T ++M
Sbjct: 93  NVKTMLSIGGGSSNPTNFASMASQPNSRKSFVDSSITLARNNGFDGLDLDWEYPSTPTEM 152

Query: 122 AAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMTY 181
            +F TLLTEWRAAV +ES SS  P+LLLSAAVFRSS+YY  P+P SS++ +LDWINVM+Y
Sbjct: 153 NSFGTLLTEWRAAVVKESVSSGKPRLLLSAAVFRSSNYYGTPLPVSSISNSLDWINVMSY 212

Query: 182 DFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQLQ 241
           DFYGP WSA ST  PAAL+ S    QVN +  I  WI SG PA+KI +G PFYGWAW+L 
Sbjct: 213 DFYGPGWSASSTAPPAALFGS--GGQVNGNAGIREWIQSGMPANKIAIGFPFYGWAWRL- 272

Query: 242 NPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSGT 301
             + N NG YAPANG AA     + G+I+Y+ IK +FI  NRV P+Y++ +VSNYV+SGT
Sbjct: 273 -VDQNKNGFYAPANG-AATGAGISGGAISYRDIK-TFIAANRVTPVYNATVVSNYVYSGT 332

Query: 302 TWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           TWIGYDD QS+AAKVSYA    L+GYF WQ+AADDD+ TLSRT +
Sbjct: 333 TWIGYDDKQSIAAKVSYANANGLWGYFGWQIAADDDSSTLSRTAS 371

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BLAST of Spg020674 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.4e-99
Identity = 186/338 (55.03%), Postives = 234/338 (69.23%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           +K  YW   S+  V DI+SSLFTH+FCAFA+L+  TN+VT+ S    +FSTFT+TV+R+N
Sbjct: 28  VKASYWFPASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQRRN 87

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P VKTLLSIGGG A    +ASMAS P SR+SFI SSI +ARSY FHGLDLDWEYP + ++
Sbjct: 88  PSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSATE 147

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           M  F TLL EWR+AV  E+ SS  P+LLL+AAVF S++YY V  P S++A +LDW+N+M 
Sbjct: 148 MTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYYSVLYPVSAVASSLDWVNLMA 207

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS   TG PAAL+  P     + D    SWI +G PA K VLG P+YG+AW+L
Sbjct: 208 YDFYGPGWSR-VTGPPAALF-DPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYGYAWRL 267

Query: 241 QNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSG 300
              N N++  YAP  G A  P    DGSI Y QI R FI  N    +Y+S +V +Y ++G
Sbjct: 268 --TNANSHSYYAPTTGAAISP----DGSIGYGQI-RKFIVDNGATTVYNSTVVGDYCYAG 327

Query: 301 TTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDT 339
           T WIGYDD QS+  KV YAK+  L GYF+W V ADD++
Sbjct: 328 TNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNS 356

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BLAST of Spg020674 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 247.7 bits (631), Expect = 2.0e-64
Identity = 138/350 (39.43%), Postives = 205/350 (58.57%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFS----AQFSTFTETV 60
           +KG+YW+       + I++SLFTHIF AF + +K T K+    + S       +TFT T 
Sbjct: 37  VKGIYWIENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEEDSTTVATSLTTFTNTF 96

Query: 61  RRKNPDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPK 120
           + K P + TLLSIGG  + +  FA +AS P +R +FI+S+I +AR++ F G+D DWE+P 
Sbjct: 97  KTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVARTFGFDGIDFDWEFPT 156

Query: 121 TESQMAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYY----RVPIPTSSLALN 180
           T  +M     LL +WR A+  E+ S+  P LLL+AAV+ + +++    R   P  S+  N
Sbjct: 157 TTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFLSGERRMYPVDSINKN 216

Query: 181 LDWINVMTYDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIP 240
           LDW+NVM+YD  G    ++ TGAP+ ++ S  +  V+    + SWI  G    KIV+G+P
Sbjct: 217 LDWVNVMSYDLRGS--GSNVTGAPSGMFDS--KSNVSVVSGLFSWIRGGVAPEKIVMGMP 276

Query: 241 FYGWAWQLQNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEI 300
            YG +W+LQ+PN   +G+ AP  GP      G DG + Y Q+   F +      +YD E 
Sbjct: 277 LYGKSWKLQDPNV--HGIGAPNVGPG----PGVDGGMAYFQVV-DFNKQMGAKVVYDKET 336

Query: 301 VSNYVHSGTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLS 343
            S Y +SG+TWIGYDD  +V+ KV +A+ ++L GYF W    D   W +S
Sbjct: 337 GSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFWAAGYDTSDWKVS 375

[Close]

BLAST of Spg020674 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.0e-60
Identity = 133/349 (38.11%), Postives = 205/349 (58.74%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           ++  YW  G D S + I+++ FTHI  AF   +  + K+ I          F + +R ++
Sbjct: 40  VRSAYWPAGDDFSPSLIDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRHRS 99

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P VKTLLSIGGG + +  F+ +AS   +R+ FI+S+I +AR Y F G+DLDWE+P+T+  
Sbjct: 100 PPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQQD 159

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRV------PIPTSSLALNLD 180
           M     L  EW  A+  E++  R P+LLL++AV+ +S    +        PT ++   LD
Sbjct: 160 MFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAVYYNSTVRLIGKHGPRSYPTQAINKYLD 219

Query: 181 WINVMTYDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFY 240
           W + M +D++G  W  ++T   AALY S  + +++ +  + SWI SG    K+V+G+  Y
Sbjct: 220 WASPMCFDYHG-TWD-NNTDFNAALYDS--KSEISTNFGLHSWIKSGVRPEKLVMGLALY 279

Query: 241 GWAWQLQNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVS 300
           G AW+L++PN   NG+ A A GPA    T  DGS+ Y +I + F + +  N +YD   +S
Sbjct: 280 GRAWELKDPNV--NGVGAEAVGPA----TDTDGSMNYNEILK-FNKQSGANVVYDKVAIS 339

Query: 301 NYVHSGTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSR 344
            Y ++GTTWIGYDD  S+  KV +AK + L GYF W +  D D W++S+
Sbjct: 340 FYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKD-WSISK 376

[Close]

BLAST of Spg020674 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.9e-60
Identity = 127/346 (36.71%), Postives = 200/346 (57.80%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           +KG+YWL         +++SLFTHIF +F   +  T K+ I S      +TFT+T + K+
Sbjct: 35  VKGIYWLENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKTKS 94

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P   TL SIGG  + +   A +AS P +  +FI+S+I +AR++ F G+DLDWE+PK   +
Sbjct: 95  PPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNTKE 154

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDY--YRVP--IPTSSLALNLDWI 180
           M     +L +WR A+  E  ++  P LLL+AAV+ + ++  Y  P   P +S+  NLDW+
Sbjct: 155 MNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLDWV 214

Query: 181 NVMTYDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGW 240
           NVM+Y+  GP   ++ TGAP+  +    +  V+    ++SWIHSG    K+V+G+P YG 
Sbjct: 215 NVMSYELRGP--RSNKTGAPSGTF--DPKSNVSVVSGLLSWIHSGVVPEKLVMGMPLYGK 274

Query: 241 AWQLQNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNY 300
           +W+L++PN +  G  +  +GP      G +G + Y Q+   F         YD +  S Y
Sbjct: 275 SWKLRDPNVHGIGAPSVGSGP------GVNGLMAYFQV-LDFNRQKSAKVEYDVDTASVY 334

Query: 301 VHSGTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLS 343
            +SG+TWIGYD+  +V+ KV +A+ ++L GYF W    D   W ++
Sbjct: 335 SYSGSTWIGYDNPFTVSIKVGFAQALKLRGYFFWVAGLDTLDWKIA 369

[Close]

BLAST of Spg020674 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 227.3 bits (578), Expect = 2.8e-58
Identity = 137/348 (39.37%), Postives = 199/348 (57.18%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           ++  YW   SD S + I+++ FTHI+ AF      +  + I   +  +++   + +    
Sbjct: 55  VRAAYWPAWSDFSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYK-KWAPKYDGIHNIR 114

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P V TLLSIGGG   A  F+ MAS   +R SFI+S+I +AR + F+GLDLDWE+P  E  
Sbjct: 115 PRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDWEWPGDEKD 174

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRV-----PIPTSSLALNLDW 180
           M+  + LL EW  A+  E+ +SR  +LLL++AV+ +S    +       P  ++   LDW
Sbjct: 175 MSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVRAIRKYLDW 234

Query: 181 INVMTYDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYG 240
            + M +D+ G  W A+ TG  AALY       ++    I SWI SG PA K+V+G+P YG
Sbjct: 235 ASPMCFDYNG-AW-ANETGFNAALY--DPNSNISTKYGIGSWIGSGVPAEKLVMGLPLYG 294

Query: 241 WAWQLQNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSN 300
            AW+L+  +PN++G+ A A GPA       DGS+ Y +I   F +      +YD   VS 
Sbjct: 295 RAWELK--DPNDHGVGAKAVGPA----VDTDGSMDYDEI-LVFNKDTGAKVVYDEVAVSF 354

Query: 301 YVHSGTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSR 344
           Y +SGTTWIGYDD  S+  KV +A+ M L GYF W +  D D WT+S+
Sbjct: 355 YSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKD-WTISK 389

[Close]

BLAST of Spg020674 vs. ExPASy TrEMBL
Match: A0A5A7T0C8 (Chitotriosidase-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G001070 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 1.1e-118
Identity = 225/347 (64.84%), Postives = 268/347 (77.23%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           MKG+YW  GS+  ++DI+SSLFTHI+CAFA+L+  TN+VTI S  S  F +FT  +RRKN
Sbjct: 32  MKGIYWFSGSEFPLSDIDSSLFTHIYCAFADLNPNTNQVTISSSNSESFKSFTPILRRKN 91

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P+VKTLLSIGGG A A+ FASMASQPNSR+SFI SSISLARSYN  GLD+DWEYP  ++Q
Sbjct: 92  PNVKTLLSIGGGGADANAFASMASQPNSRKSFIDSSISLARSYNLSGLDIDWEYPSNQNQ 151

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           + +F TL +EWRAAVE ES+S+  P+L LSAAVFRSS+YY  P+P S LA  LDWINVM 
Sbjct: 152 INSFKTLCSEWRAAVENESQSTGKPRLFLSAAVFRSSNYYGTPLPASDLATKLDWINVMC 211

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS + T  PAALY S    QVNC+  I SWI SGFPA+KIV+G+PFYGWAW+L
Sbjct: 212 YDFYGPGWSPNFTAPPAALYGS--SGQVNCNTGITSWIQSGFPANKIVIGMPFYGWAWRL 271

Query: 241 QNPNPNNNGLYAPANGPAAIPETGND-GSITYKQIKRSFIETNRVNPMYDSEIVSNYVHS 300
              + + NGLYAPANG A    TG D G+ITYK I   F + N VN +Y+  +V+NYV S
Sbjct: 272 --VSQSKNGLYAPANGAAT--GTGIDGGAITYKGI-NEFKKRNGVNGVYNGTVVTNYVRS 331

Query: 301 GTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           GTTWIGYDD +SVAAKV YAK+  LFGYFAWQVAADD+ +TLSR GT
Sbjct: 332 GTTWIGYDDKESVAAKVGYAKKKGLFGYFAWQVAADDN-FTLSRLGT 370

[Close]

BLAST of Spg020674 vs. ExPASy TrEMBL
Match: A0A6J1IEI8 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471964 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.9e-118
Identity = 220/345 (63.77%), Postives = 271/345 (78.55%), Query Frame = 0


Query: 2   KGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKNP 61
           KGVYW  GS+  VA+I S+LFTHIFCAFA+L+  TN+VTI S  +A+FSTFT+TVR+KNP
Sbjct: 33  KGVYWFSGSEFPVANINSALFTHIFCAFADLNTNTNQVTISSSNAAKFSTFTQTVRQKNP 92

Query: 62  DVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQM 121
           +VKT+LSIGGG++   +FASMASQPNSR+SF+ SSI+LAR+  F GLDLDWEYP T ++M
Sbjct: 93  NVKTMLSIGGGSSNPTNFASMASQPNSRKSFVDSSITLARNNGFDGLDLDWEYPSTPTEM 152

Query: 122 AAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMTY 181
            +F TLLTEWRAAV +ES SS  P+LLLSAAVFRSS+YY  P+P SS++ +LDWINVM+Y
Sbjct: 153 NSFGTLLTEWRAAVVKESVSSGKPRLLLSAAVFRSSNYYGTPLPVSSISNSLDWINVMSY 212

Query: 182 DFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQLQ 241
           DFYGP WSA ST  PAAL+ S    QVN +  I  WI SG PA+KI +G PFYGWAW+L 
Sbjct: 213 DFYGPGWSASSTAPPAALFGS--GGQVNGNAGIREWIQSGMPANKIAIGFPFYGWAWRL- 272

Query: 242 NPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSGT 301
             + N NG YAPANG AA     + G+I+Y+ IK +FI  NRV P+Y++ +VSNYV+SGT
Sbjct: 273 -VDQNKNGFYAPANG-AATGAGISGGAISYRDIK-TFIAANRVTPVYNATVVSNYVYSGT 332

Query: 302 TWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           TWIGYDD QS+AAKVSYA    L+GYF WQ+AADDD+ TLSRT +
Sbjct: 333 TWIGYDDKQSIAAKVSYANANGLWGYFGWQIAADDDSSTLSRTAS 371

[Close]

BLAST of Spg020674 vs. ExPASy TrEMBL
Match: A0A6J1EJC0 (chitotriosidase-1-like OS=Cucurbita moschata OX=3662 GN=LOC111433121 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.9e-118
Identity = 221/345 (64.06%), Postives = 271/345 (78.55%), Query Frame = 0


Query: 2   KGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKNP 61
           KGVYW  GS+  VA+I S+LFTHIFCAFA+L+  TN+VTI S  +A+FSTFT+TVR+KNP
Sbjct: 31  KGVYWFSGSEFPVANINSALFTHIFCAFADLNTNTNQVTISSSNAAKFSTFTQTVRQKNP 90

Query: 62  DVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQM 121
           +VKT+LSIGGG++   +FASMASQ  SR+SFI SSI+LAR+  F GLDLDWEYP T ++M
Sbjct: 91  NVKTMLSIGGGSSNPTNFASMASQSGSRKSFIDSSITLARNNGFDGLDLDWEYPSTPTEM 150

Query: 122 AAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMTY 181
            +F TLLTEWRAAV +ES SS  P+LLLSAAVFRSS+YY  P+P SS++ +LDWINVM+Y
Sbjct: 151 NSFGTLLTEWRAAVVKESVSSGKPRLLLSAAVFRSSNYYGTPLPVSSISNSLDWINVMSY 210

Query: 182 DFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQLQ 241
           DFYGP WSA ST  PAAL+ S    QVN +  I  WI SG PA+KI +G PFYGWAW+L 
Sbjct: 211 DFYGPGWSASSTAPPAALFGS--GGQVNGNAGIREWIQSGMPANKIAIGFPFYGWAWRL- 270

Query: 242 NPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSGT 301
             + N NG YAPANG AA     + G+ITY+ IK +FI TNR  P+Y++ +VSNYV+SGT
Sbjct: 271 -VDQNKNGFYAPANG-AATGAGISGGAITYRDIK-TFIATNRATPVYNATVVSNYVYSGT 330

Query: 302 TWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           TWIGYDD QS+AAKVSYA    L+GYFAWQ+AADDD++TLSRT +
Sbjct: 331 TWIGYDDKQSIAAKVSYANANGLWGYFAWQIAADDDSFTLSRTAS 369

[Close]

BLAST of Spg020674 vs. ExPASy TrEMBL
Match: A0A5D3BVX4 (Chitotriosidase-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00540 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 5.5e-118
Identity = 224/347 (64.55%), Postives = 267/347 (76.95%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           MKG+YW  GS+  ++DI+SSLFTHI+CAFA+L+  TN+VTI S  S  F +FT  +RRKN
Sbjct: 32  MKGIYWFSGSEFPLSDIDSSLFTHIYCAFADLNPNTNQVTISSSNSESFKSFTPILRRKN 91

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P+VKTLLSIGGG A A+ FASMASQPNSR+SFI SSISLARSYN  GLD+DWEYP  ++Q
Sbjct: 92  PNVKTLLSIGGGGAEANAFASMASQPNSRKSFIDSSISLARSYNLSGLDIDWEYPSNQNQ 151

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           + +F TL +EWRAAVE ES+S+  P+L LSAAVFRSS+YY  P+P S LA  LDWINVM 
Sbjct: 152 INSFKTLCSEWRAAVENESQSTGKPRLFLSAAVFRSSNYYGTPLPASDLATKLDWINVMC 211

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS + T  PAALY S    QVNC+  I SWI SGFPA+KIV+G+PFYGWAW+L
Sbjct: 212 YDFYGPGWSPNFTAPPAALYGS--SGQVNCNTGITSWIQSGFPANKIVIGMPFYGWAWRL 271

Query: 241 QNPNPNNNGLYAPANGPAAIPETGND-GSITYKQIKRSFIETNRVNPMYDSEIVSNYVHS 300
              + + NGLYAPANG A    TG D G+ITYK I   F + N VN +Y+  +V+NYV S
Sbjct: 272 --VSQSKNGLYAPANGAAT--GTGIDGGAITYKGI-NEFKKRNGVNGVYNGTVVTNYVRS 331

Query: 301 GTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           G TWIGYDD +SVAAKV YAK+  LFGYFAWQVAADD+ +TLSR GT
Sbjct: 332 GRTWIGYDDKESVAAKVGYAKKKGLFGYFAWQVAADDN-FTLSRLGT 370

[Close]

BLAST of Spg020674 vs. ExPASy TrEMBL
Match: A0A1S3B8L9 (chitotriosidase-1-like OS=Cucumis melo OX=3656 GN=LOC103487371 PE=4 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.2e-117
Identity = 223/344 (64.83%), Postives = 266/344 (77.33%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           MKG+YW  GS+  ++DI+SSLFTHI+CAFA+L+  TN+VTI S  S  F +FT  +RRKN
Sbjct: 32  MKGIYWFSGSEFPLSDIDSSLFTHIYCAFADLNPNTNQVTISSSNSESFKSFTPILRRKN 91

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P+VKTLLSIGGG A A+ FASMASQPNSR+SFI SSISLARSYN  GLD+DWEYP  ++Q
Sbjct: 92  PNVKTLLSIGGGGADANAFASMASQPNSRKSFIDSSISLARSYNLSGLDIDWEYPSNQNQ 151

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           + +F TL +EWRAAVE ES+S+  P+L LSAAVFRSS+YY  P+P S LA  LDWINVM 
Sbjct: 152 INSFKTLCSEWRAAVENESQSTGKPRLFLSAAVFRSSNYYGTPLPASDLATKLDWINVMC 211

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS + T  PAALY S    QVNC+  I SWI SGFPA+KIV+G+PFYGWAW+L
Sbjct: 212 YDFYGPGWSPNFTAPPAALYGS--SGQVNCNTGITSWIQSGFPANKIVIGMPFYGWAWRL 271

Query: 241 QNPNPNNNGLYAPANGPAAIPETGND-GSITYKQIKRSFIETNRVNPMYDSEIVSNYVHS 300
              + + NGLYAPANG A    TG D G+ITYK I   F + N VN +Y+  +V+NYV S
Sbjct: 272 --VSQSKNGLYAPANGAAT--GTGIDGGAITYKGI-NEFKKRNGVNGVYNGTVVTNYVRS 331

Query: 301 GTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSR 344
           GTTWIGYDD +SVAAKV YAK+  LFGYFAWQVAADD+ +TLSR
Sbjct: 332 GTTWIGYDDKESVAAKVGYAKKKGLFGYFAWQVAADDN-FTLSR 367

[Close]

BLAST of Spg020674 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 361.7 bits (927), Expect = 6.7e-100
Identity = 186/338 (55.03%), Postives = 234/338 (69.23%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           +K  YW   S+  V DI+SSLFTH+FCAFA+L+  TN+VT+ S    +FSTFT+TV+R+N
Sbjct: 28  VKASYWFPASEFPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQRRN 87

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P VKTLLSIGGG A    +ASMAS P SR+SFI SSI +ARSY FHGLDLDWEYP + ++
Sbjct: 88  PSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSSATE 147

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           M  F TLL EWR+AV  E+ SS  P+LLL+AAVF S++YY V  P S++A +LDW+N+M 
Sbjct: 148 MTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYYSVLYPVSAVASSLDWVNLMA 207

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS   TG PAAL+  P     + D    SWI +G PA K VLG P+YG+AW+L
Sbjct: 208 YDFYGPGWSR-VTGPPAALF-DPSNAGPSGDAGTRSWIQAGLPAKKAVLGFPYYGYAWRL 267

Query: 241 QNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSG 300
              N N++  YAP  G A  P    DGSI Y QI R FI  N    +Y+S +V +Y ++G
Sbjct: 268 --TNANSHSYYAPTTGAAISP----DGSIGYGQI-RKFIVDNGATTVYNSTVVGDYCYAG 327

Query: 301 TTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDT 339
           T WIGYDD QS+  KV YAK+  L GYF+W V ADD++
Sbjct: 328 TNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDNS 356

[Close]

BLAST of Spg020674 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 338.6 bits (867), Expect = 6.1e-93
Identity = 175/346 (50.58%), Postives = 232/346 (67.05%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           +K  YW   +D    DI+SSLFTH+FC FA+L+  + ++TI +   A F  FTETV+++N
Sbjct: 7   VKASYWFPATDFPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTETVQQRN 66

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P VKTLLSIGGGNA  D FASMAS P+SR SFI S+I++ARSY FHGLDLDWEYP+ E +
Sbjct: 67  PHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYPRNEEE 126

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           M  F  LL EWR+AVE ES SS    L+L+AAV+ SS+Y  VP P  +++ +LDWIN+M 
Sbjct: 127 MYDFGKLLEEWRSAVEAESNSSGTTALILTAAVYYSSNYQGVPYPVLAISNSLDWINLMA 186

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFYGP WS   TG PA+LY  P   + + D  +  W  +G PA K VLG P+YGWAW L
Sbjct: 187 YDFYGPGWST-VTGPPASLY-LPTDGR-SGDSGVRDWTEAGLPAKKAVLGFPYYGWAWTL 246

Query: 241 QNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSG 300
            +P+   NG  A   GPA      +DG I+Y+Q+ +++I  N    ++D  +V +Y ++G
Sbjct: 247 ADPDV--NGYDANTTGPA----ISDDGEISYRQL-QTWIVDNGATKVHDDMMVGDYCYAG 306

Query: 301 TTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           TTWIGYD  +S+  KV YAK+  L GYF+W V  DD++  LS  G+
Sbjct: 307 TTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDNS-ELSSAGS 341

[Close]

BLAST of Spg020674 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 323.6 bits (828), Expect = 2.0e-88
Identity = 174/346 (50.29%), Postives = 229/346 (66.18%), Query Frame = 0


Query: 1   MKGVYWLCGSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKN 60
           +K  YW   S+  +A I+SSLFTH+FCAFA+++  T +V + S+   +FSTFT+TVRR+N
Sbjct: 26  VKATYWFAESESPLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVRRRN 85

Query: 61  PDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQ 120
           P VKTLLSIGG       FASMAS P SR+ FI SSI LARS  FHGLDL+W+YP   ++
Sbjct: 86  PTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSITTE 145

Query: 121 MAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMT 180
           M  F  LL EWR AVE E+RSS  P+LLL+AAVF S  YY V  P +++A +LDW+N++ 
Sbjct: 146 MDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVFYSYSYYSVLHPVNAVADSLDWVNLVA 205

Query: 181 YDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAWQL 240
           YDFY    S+  T +PA LY  P+    + D  + +W  +G PA K VLG P YG+AW L
Sbjct: 206 YDFY-ESGSSRVTCSPAPLY-DPITTGPSGDAGVRAWTQAGLPAKKAVLGFPLYGYAWCL 265

Query: 241 QNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSG 300
              +  N+  YA ++GPA  P    DGSI Y QI+R FI  N+   +Y+S +V NY ++ 
Sbjct: 266 --TDAKNHNYYANSSGPAISP----DGSIGYDQIRR-FIVDNKATMVYNSNLVQNYCYAK 325

Query: 301 TTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
            TWIGYDD QS+  KV YAK+  L GYF+W + ADD++  LSR G+
Sbjct: 326 KTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDNS-RLSRAGS 361

[Close]

BLAST of Spg020674 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 297.7 bits (761), Expect = 1.2e-80
Identity = 163/350 (46.57%), Postives = 220/350 (62.86%), Query Frame = 0


Query: 1   MKGVYWLC--GSDVSVADIESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRR 60
           +K  YW+    +D    +I+S+ FTH+FCAFA++D +T++VTI +  S Q S+FT TV+ 
Sbjct: 14  VKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTVKD 73

Query: 61  KNPDVKTLLSIGGGNATADDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTE 120
           KN DV+TLLSIGG +A     ASMAS   +R++FI SSI +AR  +F+GLDL WEYP  +
Sbjct: 74  KNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSND 133

Query: 121 SQMAAFSTLLTEWRAAVERESRSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINV 180
            +MA F  L+ EWRAAV  ES  +    LLL+AAV+ S DYY    P  ++A NLD++N+
Sbjct: 134 VEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDYYGEEYPVQAIADNLDFVNI 193

Query: 181 MTYDFYGPLWSADSTGAPAALYRSPMQWQVNCDEAIMSWIHSGFPASKIVLGIPFYGWAW 240
           M YDFYGP WS   TG PAAL+        + D  +  W+ +  PA K VLG  + GWAW
Sbjct: 194 MAYDFYGPGWS-PVTGPPAALFDPSNPAGRSGDSGLSKWLEAKLPAKKAVLGFSYCGWAW 253

Query: 241 QLQNPNPNNNGLYAPANGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVH 300
            L+  +  NNG  A  +G A      +DGSITY +I R++I  N     +D  ++  Y +
Sbjct: 254 TLE--DAENNGYDAATDGAA----ISSDGSITYAKI-RNYIIDNGAATFHDPAVIGFYCY 313

Query: 301 SGTTWIGYDDTQSVAAKVSYAKRMELFGYFAWQVAADDDTWTLSRTGTYS 349
            GTTWIGYDD QS+ +KV YAK   L GYF+W V AD +   LSR G++S
Sbjct: 314 VGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGADYNC-GLSRAGSFS 354

[Close]

BLAST of Spg020674 vs. TAIR 10
Match: AT4G19720.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 286.2 bits (731), Expect = 3.6e-77
Identity = 159/332 (47.89%), Postives = 209/332 (62.95%), Query Frame = 0


Query: 17  IESSLFTHIFCAFANLDKTTNKVTIPSQFSAQFSTFTETVRRKNPDVKTLLSIGGGNATA 76
           I+S+LFTH+FCAFA+LD  TN V +      +FS FT+ V++KNP V+TLLSIGG NA  
Sbjct: 33  IDSTLFTHLFCAFADLDPQTNSVVVSGAHEQEFSNFTKIVKKKNPHVQTLLSIGGRNADK 92

Query: 77  DDFASMASQPNSRQSFIHSSISLARSYNFHGLDLDWEYPKTESQMAAFSTLLTEWRAAVE 136
             FASMAS P SR+SFI S+IS AR Y F GLDL W+YPK + +M  F  LL +WR A+E
Sbjct: 93  SAFASMASNPTSRKSFIWSAISSARYYRFDGLDLVWKYPKDDVEMRNFGQLLEQWREAIE 152

Query: 137 RES-RSSRMPQLLLSAAVFRSSDYYRVPIPTSSLALNLDWINVMTYDFYGPLWSADSTGA 196
            ++ R+ RMP LLL+AAV+ S  Y  V  P   +   LDW+N++ YDFY    S+ + G 
Sbjct: 153 DDAERTERMP-LLLTAAVYYSPVYDSVSYPIREIKKKLDWVNLIAYDFYS---SSTTIGP 212

Query: 197 PAALYRSPMQWQVNC-DEAIMSWIHSGFPASKIVLGIPFYGWAWQLQNPNPNNNGLYAPA 256
           PAAL+  P   +  C D  +  WI +G PA K VLG P+ GW W L + N       A  
Sbjct: 213 PAALF-DPSNPKGPCGDYGLKEWIKAGLPAKKAVLGFPYVGWTWSLGSGNDAATSRVA-- 272

Query: 257 NGPAAIPETGNDGSITYKQIKRSFIETNRVNPMYDSEIVSNYVHSGTTWIGYDDTQSVAA 316
                   T  +GSI Y QIKR  ++ ++  P++DS +V +Y  +GT+ IGYDD QSV A
Sbjct: 273 --------TSAEGSINYDQIKRLIVD-HKARPVFDSTVVGDYCFAGTSLIGYDDHQSVVA 332

Query: 317 KVSYAKRMELFGYFAWQVAADDDTWTLSRTGT 347
           KV YAK+  L GYF+W V ADD+ + LSR G+
Sbjct: 333 KVKYAKQKGLLGYFSWHVGADDN-FGLSRAGS 347

The following BLAST results are available for this feature:

BLAST of Spg020674 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879961.1	1.9e-125	68.79	class V chitinase-like [Benincasa hispida]	[more]
XP_023529986.1	7.9e-119	64.35	chitotriosidase-1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6587842.1	7.9e-119	64.64	Class V chitinase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAA0035051.1	2.3e-118	64.84	chitotriosidase-1-like [Cucumis melo var. makuwa]	[more]
XP_022973419.1	3.9e-118	63.77	chitotriosidase-1-like [Cucurbita maxima]	[more]

BLAST of Spg020674 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81862	9.4e-99	55.03	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1	[more]
U5N4E3	2.0e-64	39.43	Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2	[more]
A0A072VEP0	3.0e-60	38.11	Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1	[more]
A0A072UR65	3.9e-60	36.71	Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1	[more]
A0A1B1J8Z2	2.8e-58	39.37	Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2	[more]

BLAST of Spg020674 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T0C8	1.1e-118	64.84	Chitotriosidase-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5...	[more]
A0A6J1IEI8	1.9e-118	63.77	chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111471964 PE=3 SV=1	[more]
A0A6J1EJC0	1.9e-118	64.06	chitotriosidase-1-like OS=Cucurbita moschata OX=3662 GN=LOC111433121 PE=3 SV=1	[more]
A0A5D3BVX4	5.5e-118	64.55	Chitotriosidase-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1...	[more]
A0A1S3B8L9	1.2e-117	64.83	chitotriosidase-1-like OS=Cucumis melo OX=3656 GN=LOC103487371 PE=4 SV=1	[more]

BLAST of Spg020674 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19810.1	6.7e-100	55.03	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19800.1	6.1e-93	50.58	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19820.1	2.0e-88	50.29	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19750.1	1.2e-80	46.57	Glycosyl hydrolase family protein with chitinase insertion domain	[more]
AT4G19720.1	3.6e-77	47.89	Glycosyl hydrolase family protein with chitinase insertion domain	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011583	Chitinase II	SMART	SM00636	2g34	coord: 1..335 e-value: 3.7E-94 score: 328.8
IPR029070	Chitinase insertion domain superfamily	GENE3D	3.10.50.10		coord: 233..306 e-value: 8.8E-108 score: 362.5
IPR029070	Chitinase insertion domain superfamily	SUPERFAMILY	54556	Chitinase insertion domain	coord: 234..307
IPR001223	Glycoside hydrolase family 18, catalytic domain	PFAM	PF00704	Glyco_hydro_18	coord: 17..335 e-value: 8.1E-81 score: 272.1
IPR001223	Glycoside hydrolase family 18, catalytic domain	PROSITE	PS51910	GH18_2	coord: 1..349 score: 63.153793
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 15..336 e-value: 8.8E-108 score: 362.5
None	No IPR available	PANTHER	PTHR11177:SF351	CHITINASE-LIKE PROTEIN-RELATED	coord: 2..345
None	No IPR available	PANTHER	PTHR11177	CHITINASE	coord: 2..345
None	No IPR available	CDD	cd02879	GH18_plant_chitinase_class_V	coord: 2..341 e-value: 2.73875E-130 score: 372.468
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 11..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg020674.1	Spg020674.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006032	chitin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding