Spg020233 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg020233
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	E3 ubiquitin-protein ligase RNF181-like
Location	scaffold1: 27415872 .. 27416603 (-)
RNA-Seq Expression	Spg020233
Synteny	Spg020233

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTCGCTACTTCCAGACCGATCCAATATCGTCTTTACGGCGACAGCAACGCCGCTTCCGGCGAGCAACCCTATCAAGTTCTGCAACTCCGATTGCGCTACGTCCACCGTCTGATCGCTGGATCCCCGTCCGTCGGAGTTCCGTACCGAATCCTCCATGAAACTGCGCCGGCTCCACTAGACGATCTTCTGTTCCCGCTCCCTCTGCGCGAACTCCCTGACCCGGTGTTCTTTCAGATCTACGTGGAGGAGTTGCTGTCCAGCGTCGGCATCCGCGCCGCCGCTCGCGAAGTCATGGCCCGGCAGATCGCGTCGTACGTGTTCGGAATGGCGGGCGGAGACAGAGAGATGAACTTCCTCATCGTGGCGGTGGTGAATAACATCCAACAGACGTGGATGATGGAGGCAAACCCGGAGGAAGAGAGCAATGGCGGTGCAGTGGGGGAGGAGACGGTGATTCTGGAAGAAGCTGCTAGGCCGGCGAGGAGGAGGGGAGTGGCGGAGTGGGCGATGGAGAGGCTGAGGAAGGAGAAATTGGAAGAGAATGAGAATGGAGAAGAATTGGGAGATTGCAGTATCTGTTTGGATGAAATGAATTGCAATTGCAAGAAGAAGGAGGTGATAAAGATCCCCTGCGGCCATGCCTATCATCATTCATGCATCTCCAAATGGTTCCACAATCACAATTCATGCCCCTTGTGCAGAAGCCCACTCCCATTGGAGGACTGA

mRNA sequence

ATGTCTTTCGCTACTTCCAGACCGATCCAATATCGTCTTTACGGCGACAGCAACGCCGCTTCCGGCGAGCAACCCTATCAAGTTCTGCAACTCCGATTGCGCTACGTCCACCGTCTGATCGCTGGATCCCCGTCCGTCGGAGTTCCGTACCGAATCCTCCATGAAACTGCGCCGGCTCCACTAGACGATCTTCTGTTCCCGCTCCCTCTGCGCGAACTCCCTGACCCGGTGTTCTTTCAGATCTACGTGGAGGAGTTGCTGTCCAGCGTCGGCATCCGCGCCGCCGCTCGCGAAGTCATGGCCCGGCAGATCGCGTCGTACGTGTTCGGAATGGCGGGCGGAGACAGAGAGATGAACTTCCTCATCGTGGCGGTGGTGAATAACATCCAACAGACGTGGATGATGGAGGCAAACCCGGAGGAAGAGAGCAATGGCGGTGCAGTGGGGGAGGAGACGGTGATTCTGGAAGAAGCTGCTAGGCCGGCGAGGAGGAGGGGAGTGGCGGAGTGGGCGATGGAGAGGCTGAGGAAGGAGAAATTGGAAGAGAATGAGAATGGAGAAGAATTGGGAGATTGCAGTATCTGTTTGGATGAAATGAATTGCAATTGCAAGAAGAAGGAGGTGATAAAGATCCCCTGCGGCCATGCCTATCATCATTCATGCATCTCCAAATGGTTCCACAATCACAATTCATGCCCCTTGTGCAGAAGCCCACTCCCATTGGAGGACTGA

Coding sequence (CDS)

ATGTCTTTCGCTACTTCCAGACCGATCCAATATCGTCTTTACGGCGACAGCAACGCCGCTTCCGGCGAGCAACCCTATCAAGTTCTGCAACTCCGATTGCGCTACGTCCACCGTCTGATCGCTGGATCCCCGTCCGTCGGAGTTCCGTACCGAATCCTCCATGAAACTGCGCCGGCTCCACTAGACGATCTTCTGTTCCCGCTCCCTCTGCGCGAACTCCCTGACCCGGTGTTCTTTCAGATCTACGTGGAGGAGTTGCTGTCCAGCGTCGGCATCCGCGCCGCCGCTCGCGAAGTCATGGCCCGGCAGATCGCGTCGTACGTGTTCGGAATGGCGGGCGGAGACAGAGAGATGAACTTCCTCATCGTGGCGGTGGTGAATAACATCCAACAGACGTGGATGATGGAGGCAAACCCGGAGGAAGAGAGCAATGGCGGTGCAGTGGGGGAGGAGACGGTGATTCTGGAAGAAGCTGCTAGGCCGGCGAGGAGGAGGGGAGTGGCGGAGTGGGCGATGGAGAGGCTGAGGAAGGAGAAATTGGAAGAGAATGAGAATGGAGAAGAATTGGGAGATTGCAGTATCTGTTTGGATGAAATGAATTGCAATTGCAAGAAGAAGGAGGTGATAAAGATCCCCTGCGGCCATGCCTATCATCATTCATGCATCTCCAAATGGTTCCACAATCACAATTCATGCCCCTTGTGCAGAAGCCCACTCCCATTGGAGGACTGA

Protein sequence

MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAPLDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNFLIVAVVNNIQQTWMMEANPEEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRKEKLEENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLPLED

Homology

[Close]

BLAST of Spg020233 vs. NCBI nr
Match: XP_023518048.1 (uncharacterized protein LOC111781594 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.5 bits (589), Expect = 7.8e-57
Identity = 135/247 (54.66%), Postives = 169/247 (68.42%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSF TSRPIQYR+YG ++AASGEQPYQVLQL+L Y+ R I+ SP  GV Y  L ETAP  
Sbjct: 1   MSFITSRPIQYRIYGYTSAASGEQPYQVLQLQLHYIRRDISVSPLSGVTY--LQETAPIL 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
           L +LLFPLPL+EL DPV  Q+YV +LLS + +R+ A E +A +I SY   MAGG+RE +F
Sbjct: 61  LRNLLFPLPLQELHDPVILQVYVAQLLSLLNVRSPACEAIAERIVSYTILMAGGNRETSF 120

Query: 121 LIVAVVNNIQQ-TWMMEANP--EEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRK 180
             V  ++ I+  TWM E +   +  SNG A GEETVILEE ARP                
Sbjct: 121 HTVVDLDFIRLITWMEENHGLFQGWSNGSAEGEETVILEE-ARPV--------------T 180

Query: 181 EKLEENENGEELGDCSICLDEMNCN-----CKKKEVIKIPCGHAYHHSCISKWFHNHNSC 240
           EK+E  ENGEE+GDCSICL+E+N N      K++EVI++ CGH YH SCI  W H+++SC
Sbjct: 181 EKVE--ENGEEMGDCSICLEELNGNGNGEGKKREEVIRMQCGHVYHESCIFNWLHHNSSC 228

[Close]

BLAST of Spg020233 vs. NCBI nr
Match: KAG6595648.1 (hypothetical protein SDJN03_12201, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 229.2 bits (583), Expect = 3.9e-56
Identity = 134/249 (53.82%), Postives = 168/249 (67.47%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSF TSRPIQYR+YG ++AASGEQPYQVLQL+LRY  R I+ SP  GV Y  L ETAP  
Sbjct: 1   MSFVTSRPIQYRIYGYTSAASGEQPYQVLQLQLRYFRREISVSPLSGVTY--LQETAPIL 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
           L +LLFPL L+EL DPVF Q+YV +LLS + +R+  REV+A +I SY   +AGG+RE +F
Sbjct: 61  LRNLLFPLRLQELHDPVFLQVYVAQLLSLLNVRSPVREVIAERIVSYTLLVAGGNRETSF 120

Query: 121 LIVAVVNNIQQ-TWMMEANP--EEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRK 180
             V  ++ I+  TWM E +   +  SN  A GEET ILEE ARP                
Sbjct: 121 HTVLELDYIRVITWMEENHGLFQGWSNDNAAGEETAILEE-ARPV--------------T 180

Query: 181 EKLEENENGEELGDCSICLDEMNCN-------CKKKEVIKIPCGHAYHHSCISKWFHNHN 240
           EK+E  ENGEE+GDCSICL+E+N N        K++EVI++ CGH YH SCI  W H+++
Sbjct: 181 EKVE--ENGEEMGDCSICLEELNGNGNGNGEGKKREEVIRMQCGHVYHESCIFNWLHHNS 230

[Close]

BLAST of Spg020233 vs. NCBI nr
Match: KAG6595650.1 (hypothetical protein SDJN03_12203, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 229.2 bits (583), Expect = 3.9e-56
Identity = 134/249 (53.82%), Postives = 168/249 (67.47%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSF TSRPIQYR+YG ++AASGEQPYQVLQL+LRY  R I+ SP  GV Y  L ETAP  
Sbjct: 1   MSFVTSRPIQYRIYGYTSAASGEQPYQVLQLQLRYFRREISVSPLSGVTY--LQETAPIL 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
           L +LLFPL L+EL DPVF Q+YV +LLS + +R+  REV+A +I SY   +AGG+RE +F
Sbjct: 61  LRNLLFPLRLQELHDPVFLQVYVAQLLSLLNVRSPVREVIAERIVSYTLLVAGGNRETSF 120

Query: 121 LIVAVVNNIQQ-TWMMEANP--EEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRK 180
             V  ++ I+  TWM E +   +  SN  A GEET ILEE ARP                
Sbjct: 121 HTVLELDYIRLITWMEENHGLFQGWSNDNAAGEETAILEE-ARPV--------------T 180

Query: 181 EKLEENENGEELGDCSICLDEMNCN-------CKKKEVIKIPCGHAYHHSCISKWFHNHN 240
           EK+E  ENGEE+GDCSICL+E+N N        K++EVI++ CGH YH SCI  W H+++
Sbjct: 181 EKVE--ENGEEMGDCSICLEELNGNGNGKGEGKKREEVIRMQCGHVYHESCIFNWLHHNS 230

[Close]

BLAST of Spg020233 vs. NCBI nr
Match: XP_016899530.1 (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29842.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 210.3 bits (534), Expect = 1.9e-50
Identity = 116/243 (47.74%), Postives = 158/243 (65.02%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSFA S PIQYRLYG     +G     +L++R+ +  ++I+ SP  G P++ILH+T   P
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNG-----ILEVRISFFEQMISLSPVTGRPHQILHQTPRIP 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
             + L  L LR+L  P+F   Y+ + LSS+ IR AA + MARQIAS++  M   + + NF
Sbjct: 61  YHNALVYLTLRQLQSPLF---YITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNF 120

Query: 121 LIVAVVNNIQQTWMMEANPEEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRKEKL 180
            I+A V+ I+  W+      EE + GA  EETV+ +EA  PA +RGV      + R E+L
Sbjct: 121 HIIAEVDFIRVIWL----ELEEQSMGAGAEETVVFDEAP-PALKRGVG-----KARGERL 180

Query: 181 EENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLP 240
           +  E  E+LGDCSICLDE+  +C+K+EVI+IPCGH YH SCI KW  NHNSCPLCR PLP
Sbjct: 181 KSEEKVEDLGDCSICLDEL--SCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLP 223

Query: 241 LED 244
           L++
Sbjct: 241 LKE 223

[Close]

BLAST of Spg020233 vs. NCBI nr
Match: XP_004144954.1 (uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical protein Csa_020356 [Cucumis sativus])

HSP 1 Score: 207.2 bits (526), Expect = 1.6e-49
Identity = 117/243 (48.15%), Postives = 154/243 (63.37%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSFA S PIQYRLY      +G     VLQ+ + ++ ++I+ SP  G P+ +LH+T   P
Sbjct: 1   MSFAISNPIQYRLYASGTGGNG-----VLQVNISFIQQIISVSPLTGRPHEVLHQTPRIP 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
             + LF L LR+L  P+F   Y+ ++LSS+ IR AA + MAR+IAS++  M   + E NF
Sbjct: 61  YHNALFYLSLRQLQSPLF---YITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNF 120

Query: 121 LIVAVVNNIQQTWMMEANPEEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRKEKL 180
            I+A V+ I+  W+          GGA  EETV+L+EA  PA +RGV     ERLR E  
Sbjct: 121 HILAEVDFIRLIWL-----PSMGGGGAGAEETVVLDEAP-PAVKRGVGVARGERLRSE-- 180

Query: 181 EENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLP 240
              E  EELGDCSICLDE+  +C+K+EV++IPCGH YH SCI KW  NHNSCPLCR PL 
Sbjct: 181 ---EKMEELGDCSICLDEL--SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLH 222

Query: 241 LED 244
            +D
Sbjct: 241 HDD 222

[Close]

BLAST of Spg020233 vs. ExPASy Swiss-Prot
Match: Q9Y7K6 (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC2A9.04c PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.8e-09
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 0


Query: 192 CSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLPLE 243
           C IC D+MN N  +K+  K+PCGH +  +C+ KW  NH +CPLCR  +P E
Sbjct: 107 CPICYDDMNEN-DEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRKEVPHE 156

[Close]

BLAST of Spg020233 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.1e-08
Identity = 31/98 (31.63%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 149 GEETVILEEAARPARRRG---VAEWAMERLRKEKLEENENGEELGDCSICLDEMNCNCKK 208
           G E +I + A     R G    ++ A++ L   K+ ++    E+  C++C+DE       
Sbjct: 170 GLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE---DG 229

Query: 209 KEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLPLED 244
            +V ++PC H +H  C+  W   HNSCP+CR  LP +D
Sbjct: 230 SDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Spg020233 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.9e-08
Identity = 31/98 (31.63%), Postives = 49/98 (50.00%), Query Frame = 0


Query: 149 GEETVILEEAARPARRRG---VAEWAMERLRKEKLEENENGEELGDCSICLDEMNCNCKK 208
           G E +I + A     R G    ++ A+E L    + ++    E   C++C+D+     + 
Sbjct: 178 GLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEA 237

Query: 209 KEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLPLED 244
           K+   +PC H YH  C+  W   HNSCP+CR  LP +D
Sbjct: 238 KQ---MPCKHLYHKDCLLPWLELHNSCPVCRHELPTDD 272

[Close]

BLAST of Spg020233 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-07
Identity = 24/54 (44.44%), Postives = 34/54 (62.96%), Query Frame = 0


Query: 190 GDCSICLDEMNCNCKKKEVIK-IPCGHAYHHSCISKWFHNHNSCPLCRSPLPLE 243
           G+C ICL+E     K +E +K +PC H +H  CI KW   H SCP+CR  +P++
Sbjct: 111 GECVICLEEW----KSEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVD 160

[Close]

BLAST of Spg020233 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.5e-07
Identity = 23/52 (44.23%), Postives = 32/52 (61.54%), Query Frame = 0


Query: 192 CSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLPLED 244
           C +CL E     +++ VI++PC H +H +CI  W    NSCPLCR  LP +D
Sbjct: 88  CPVCLLEFE---EEETVIEMPCHHLFHSNCILPWLSKTNSCPLCRHELPTDD 136

[Close]

BLAST of Spg020233 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 9.0e-51
Identity = 116/243 (47.74%), Postives = 158/243 (65.02%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSFA S PIQYRLYG     +G     +L++R+ +  ++I+ SP  G P++ILH+T   P
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNG-----ILEVRISFFEQMISLSPVTGRPHQILHQTPRIP 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
             + L  L LR+L  P+F   Y+ + LSS+ IR AA + MARQIAS++  M   + + NF
Sbjct: 61  YHNALVYLTLRQLQSPLF---YITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNF 120

Query: 121 LIVAVVNNIQQTWMMEANPEEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRKEKL 180
            I+A V+ I+  W+      EE + GA  EETV+ +EA  PA +RGV      + R E+L
Sbjct: 121 HIIAEVDFIRVIWL----ELEEQSMGAGAEETVVFDEAP-PALKRGVG-----KARGERL 180

Query: 181 EENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLP 240
           +  E  E+LGDCSICLDE+  +C+K+EVI+IPCGH YH SCI KW  NHNSCPLCR PLP
Sbjct: 181 KSEEKVEDLGDCSICLDEL--SCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLP 223

Query: 241 LED 244
           L++
Sbjct: 241 LKE 223

[Close]

BLAST of Spg020233 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 9.0e-51
Identity = 116/243 (47.74%), Postives = 158/243 (65.02%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSFA S PIQYRLYG     +G     +L++R+ +  ++I+ SP  G P++ILH+T   P
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNG-----ILEVRISFFEQMISLSPVTGRPHQILHQTPRIP 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
             + L  L LR+L  P+F   Y+ + LSS+ IR AA + MARQIAS++  M   + + NF
Sbjct: 61  YHNALVYLTLRQLQSPLF---YITQFLSSLNIRPAASQSMARQIASHILQMPDRNGDRNF 120

Query: 121 LIVAVVNNIQQTWMMEANPEEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRKEKL 180
            I+A V+ I+  W+      EE + GA  EETV+ +EA  PA +RGV      + R E+L
Sbjct: 121 HIIAEVDFIRVIWL----ELEEQSMGAGAEETVVFDEAP-PALKRGVG-----KARGERL 180

Query: 181 EENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLP 240
           +  E  E+LGDCSICLDE+  +C+K+EVI+IPCGH YH SCI KW  NHNSCPLCR PLP
Sbjct: 181 KSEEKVEDLGDCSICLDEL--SCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLP 223

Query: 241 LED 244
           L++
Sbjct: 241 LKE 223

[Close]

BLAST of Spg020233 vs. ExPASy TrEMBL
Match: A0A0A0K696 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 7.6e-50
Identity = 117/243 (48.15%), Postives = 154/243 (63.37%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSFA S PIQYRLY      +G     VLQ+ + ++ ++I+ SP  G P+ +LH+T   P
Sbjct: 1   MSFAISNPIQYRLYASGTGGNG-----VLQVNISFIQQIISVSPLTGRPHEVLHQTPRIP 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
             + LF L LR+L  P+F   Y+ ++LSS+ IR AA + MAR+IAS++  M   + E NF
Sbjct: 61  YHNALFYLSLRQLQSPLF---YITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNF 120

Query: 121 LIVAVVNNIQQTWMMEANPEEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRKEKL 180
            I+A V+ I+  W+          GGA  EETV+L+EA  PA +RGV     ERLR E  
Sbjct: 121 HILAEVDFIRLIWL-----PSMGGGGAGAEETVVLDEAP-PAVKRGVGVARGERLRSE-- 180

Query: 181 EENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLP 240
              E  EELGDCSICLDE+  +C+K+EV++IPCGH YH SCI KW  NHNSCPLCR PL 
Sbjct: 181 ---EKMEELGDCSICLDEL--SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLH 222

Query: 241 LED 244
            +D
Sbjct: 241 HDD 222

[Close]

BLAST of Spg020233 vs. ExPASy TrEMBL
Match: A0A0A0K2W2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 7.6e-50
Identity = 117/243 (48.15%), Postives = 154/243 (63.37%), Query Frame = 0


Query: 1   MSFATSRPIQYRLYGDSNAASGEQPYQVLQLRLRYVHRLIAGSPSVGVPYRILHETAPAP 60
           MSFA S PIQYRLY      +G     VLQ+ + ++ ++I+ SP  G P+ +LH+T   P
Sbjct: 1   MSFAISNPIQYRLYASGTGGNG-----VLQVNISFIQQIISVSPLTGRPHEVLHQTPRIP 60

Query: 61  LDDLLFPLPLRELPDPVFFQIYVEELLSSVGIRAAAREVMARQIASYVFGMAGGDREMNF 120
             + LF L LR+L  P+F   Y+ ++LSS+ IR AA + MAR+IAS++  M   + E NF
Sbjct: 61  YHNALFYLSLRQLQSPLF---YITQILSSLNIRPAASQNMARRIASHILQMPDRNSETNF 120

Query: 121 LIVAVVNNIQQTWMMEANPEEESNGGAVGEETVILEEAARPARRRGVAEWAMERLRKEKL 180
            I+A V+ I+  W+          GGA  EETV+L+EA  PA +RGV     ERLR E  
Sbjct: 121 HILAEVDFIRLIWL-----PSMGGGGAGAEETVVLDEAP-PAVKRGVGVARGERLRSE-- 180

Query: 181 EENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLP 240
              E  EELGDCSICLDE+  +C+K+EV++IPCGH YH SCI KW  NHNSCPLCR PL 
Sbjct: 181 ---EKMEELGDCSICLDEL--SCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRKPLH 222

Query: 241 LED 244
            +D
Sbjct: 241 HDD 222

[Close]

BLAST of Spg020233 vs. ExPASy TrEMBL
Match: A0A6J1EKL4 (E3 ubiquitin-protein ligase arkadia-like OS=Cucurbita moschata OX=3662 GN=LOC111435522 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.2e-39
Identity = 102/214 (47.66%), Postives = 136/214 (63.55%), Query Frame = 0


Query: 33  LRYVHRLIAGSPSVGVPYRILHETAPAPLDDLLFPLPLRELPDPVFFQIYVEELLSSVGI 92
           +R  +RLI+ S   GVP +IL ET P P++D LF L LR+L DP+F   ++  LLS++ I
Sbjct: 1   MRSFNRLISASSFPGVPPQILFETRPTPVNDALFHLSLRQLHDPLF---HIPRLLSTLDI 60

Query: 93  RAAAREVMARQIASYVFGMAGGDREMNFLIVAVVNNIQQTWM--MEANPEEESNGGAVGE 152
            AAA EV+  +IAS +  ++ G+RE +F IV  V+ IQ  WM  +    + + NGG + E
Sbjct: 61  HAAACEVIGWKIASQIAQLSAGNRETSFHIVVEVDFIQNIWMGQVAGMRQGQGNGGGLVE 120

Query: 153 ETVILEE-AARPARRRGVAEWAMERLRKEKLEENENGEELGDCSICLDEMNCNCKKKEVI 212
           ETV+L+E  AR  +RRGV              E E  +ELGDC ICLDEM    KK+EVI
Sbjct: 121 ETVVLDELPARRRQRRGVVARV----------EVEEEKELGDCCICLDEM--MNKKREVI 180

Query: 213 KIPCGHAYHHSCISKWFHNHNSCPLCRSPLPLED 244
           + PCGH YH SCI +W + HNSCPLC+ PLPLE+
Sbjct: 181 RTPCGHVYHESCIFQWLNLHNSCPLCKRPLPLEE 199

[Close]

BLAST of Spg020233 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-09
Identity = 31/98 (31.63%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 149 GEETVILEEAARPARRRG---VAEWAMERLRKEKLEENENGEELGDCSICLDEMNCNCKK 208
           G E +I + A     R G    ++ A++ L   K+ ++    E+  C++C+DE       
Sbjct: 170 GLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFE---DG 229

Query: 209 KEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSPLPLED 244
            +V ++PC H +H  C+  W   HNSCP+CR  LP +D
Sbjct: 230 SDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of Spg020233 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.1e-08
Identity = 24/54 (44.44%), Postives = 34/54 (62.96%), Query Frame = 0


Query: 190 GDCSICLDEMNCNCKKKEVIK-IPCGHAYHHSCISKWFHNHNSCPLCRSPLPLE 243
           G+C ICL+E     K +E +K +PC H +H  CI KW   H SCP+CR  +P++
Sbjct: 111 GECVICLEEW----KSEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVD 160

[Close]

BLAST of Spg020233 vs. TAIR 10
Match: AT1G21960.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.4e-08
Identity = 33/91 (36.26%), Postives = 47/91 (51.65%), Query Frame = 0


Query: 150 EETVILEEAARPARRRGVAEWAMERLRKEKLEENENGEELGDCSICLDEMNCNCKKKEVI 209
           +ET    E  RPA +  V   A +   K+K    ++  +   C+ICL+E     K + V+
Sbjct: 120 DETTNNIELIRPANKLVVDRLARKVYNKKK----KSNSDASVCTICLEEFE---KGEIVV 179

Query: 210 KIPCGHAYHHSCISKWFHNHNSCPLCRSPLP 241
            +PCGH +   CI KWF   + CPLCR  LP
Sbjct: 180 TLPCGHEFDDGCIGKWFLKDHVCPLCRFELP 203

[Close]

BLAST of Spg020233 vs. TAIR 10
Match: AT1G18770.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.9e-08
Identity = 25/64 (39.06%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 179 KLEENENGEELGDCSICLDEMNCNCKKKEVIKIPCGHAYHHSCISKWFHNHNSCPLCRSP 238
           K+  +  GE    C ICL+E +   + + V+ +PCGH +   C+ KWF  ++SCPLCR  
Sbjct: 49  KMTTSSTGEM---CIICLEEFS---EGRRVVTLPCGHDFDDECVLKWFETNHSCPLCRFK 106

Query: 239 LPLE 243
           LP +
Sbjct: 109 LPYQ 106

[Close]

BLAST of Spg020233 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 55.1 bits (131), Expect = 9.3e-08
Identity = 29/90 (32.22%), Postives = 42/90 (46.67%), Query Frame = 0


Query: 154 ILEEAARPARRRG---VAEWAMERLRKEKLEENENGEELGDCSICLDEMNCNCKKKEVIK 213
           + E+ +    RRG       A++ L   K+ +        +C +C DE     + K+   
Sbjct: 149 LFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQ--- 208

Query: 214 IPCGHAYHHSCISKWFHNHNSCPLCRSPLP 241
           +PC H YH  CI  W   HNSCP+CR  LP
Sbjct: 209 MPCNHIYHSDCIVPWLVQHNSCPVCRQELP 235

The following BLAST results are available for this feature:

BLAST of Spg020233 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023518048.1	7.8e-57	54.66	uncharacterized protein LOC111781594 [Cucurbita pepo subsp. pepo]	[more]
KAG6595648.1	3.9e-56	53.82	hypothetical protein SDJN03_12201, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6595650.1	3.9e-56	53.82	hypothetical protein SDJN03_12203, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_016899530.1	1.9e-50	47.74	PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 ...	[more]
XP_004144954.1	1.6e-49	48.15	uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical ...	[more]

BLAST of Spg020233 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y7K6	2.8e-09	49.02	Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai...	[more]
Q8LPN7	4.1e-08	31.63	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	6.9e-08	31.63	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q9LQX2	1.5e-07	44.44	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q6AXU4	4.5e-07	44.23	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]

BLAST of Spg020233 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E1S5	9.0e-51	47.74	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S4DUZ4	9.0e-51	47.74	E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 ...	[more]
A0A0A0K696	7.6e-50	48.15	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G026750 P...	[more]
A0A0A0K2W2	7.6e-50	48.15	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G025210 P...	[more]
A0A6J1EKL4	1.2e-39	47.66	E3 ubiquitin-protein ligase arkadia-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Spg020233 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	2.9e-09	31.63	RING/U-box superfamily protein	[more]
AT1G26800.1	1.1e-08	44.44	RING/U-box superfamily protein	[more]
AT1G21960.1	1.4e-08	36.26	RING/U-box superfamily protein	[more]
AT1G18770.1	1.9e-08	39.06	RING/U-box superfamily protein	[more]
AT2G40830.2	9.3e-08	32.22	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 192..235 e-value: 4.7E-6 score: 36.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 191..236 e-value: 5.8E-11 score: 42.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 192..236 score: 12.99088
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 155..241 e-value: 1.3E-18 score: 68.4
None	No IPR available	PANTHER	PTHR45931:SF9	E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKE	coord: 83..239
None	No IPR available	PANTHER	PTHR45931	SI:CH211-59O9.10	coord: 83..239
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 175..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg020233.1	Spg020233.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044267	cellular protein metabolic process