Spg020133 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg020133 vs. NCBI nr
Match: XP_038883574.1 (E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida])

HSP 1 Score: 105.5 bits (262), Expect = 7.2e-19
Identity = 55/137 (40.15%), Postives = 78/137 (56.93%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHSWMRLIPV-------RYAVIVEDA 210
           AC+++   +A Y+ +M+ ENRE NF ++A VD     WM    +          V+ ++A
Sbjct: 89  ACEIMARQIASYIDRMINENRESNFRIIAEVDFSRVIWMEEHSIYGGDAMAEETVVFDEA 148

Query: 211 RRPR-------MEKLEEV--GEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWL 270
             PR       +E++ E+   E+LG C ICL+E  S  K +V++IPC HVY   C+ KWL
Sbjct: 149 PPPRRGVEKVAVERVRELKSQEELGDCCICLEEL-SCEKREVIRIPCGHVYHESCVFKWL 208

Query: 271 ENKNSCPLCRSPLPLED 272
              NSCPLCR PLPL D
Sbjct: 209 RKNNSCPLCRKPLPLND 224

BLAST of Spg020133 vs. NCBI nr
Match: XP_016899530.1 (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29842.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-18
Identity = 57/137 (41.61%), Postives = 76/137 (55.47%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHSWMRL------IPVRYAVIVEDA- 210
           A + +   +A +++QM   N + NFH++A VD I   W+ L            V+ ++A 
Sbjct: 88  ASQSMARQIASHILQMPDRNGDRNFHIIAEVDFIRVIWLELEEQSMGAGAEETVVFDEAP 147

Query: 211 ---------RRPRMEKLEEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWL 270
                     R    K EE  EDLG CSICLDE  S  K +V++IPC HVY   CI KWL
Sbjct: 148 PALKRGVGKARGERLKSEEKVEDLGDCSICLDEL-SCEKREVIRIPCGHVYHESCIFKWL 207

Query: 271 ENKNSCPLCRSPLPLED 272
           +N NSCPLCR PLPL++
Sbjct: 208 KNHNSCPLCRKPLPLKE 223

BLAST of Spg020133 vs. NCBI nr
Match: XP_023512244.1 (RING-H2 finger protein ATL16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 100.9 bits (250), Expect = 1.8e-17
Identity = 57/136 (41.91%), Postives = 80/136 (58.82%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHS--W-MRLIPV---------RYAV 210
           A ++I+  +A + +Q+  E+ + N H+VA VD +  S  W ++ +PV         R  V
Sbjct: 88  ASEIISAQIASFTLQLAEEHNDTNVHVVAVVDNVMESRFWRLQAVPVEQGGSAAVERREV 147

Query: 211 IVEDAR---RPRMEKL--EEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKW 270
           + E A    R RMEK+  EE  EDLG CSICLDE K     +V++ PC HVY   CI +W
Sbjct: 148 VEEVAAANGRYRMEKVEAEEEEEDLGNCSICLDEMKCEG-SEVIRTPCGHVYHESCIFRW 207

BLAST of Spg020133 vs. NCBI nr
Match: XP_022944063.1 (RING-H2 finger protein ATL16-like [Cucurbita moschata])

HSP 1 Score: 100.9 bits (250), Expect = 1.8e-17
Identity = 58/137 (42.34%), Postives = 80/137 (58.39%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHS--W-MRLIPV---------RYAV 210
           A +VI+  +A + +Q+  E+ + N H+VA VD +  S  W ++ +PV         R  V
Sbjct: 88  ASEVISAQIASFTLQLAEEHNDTNVHVVAVVDNVMESRFWRLQAVPVVQGGSAAVERREV 147

Query: 211 IVEDAR---RPRMEKL---EEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILK 270
           + E A    R RMEK+   EE  EDLG CSICLDE K     +V++ PC HVY   CI +
Sbjct: 148 VEEVAAANGRYRMEKVEAEEEEEEDLGNCSICLDEMKCEG-SEVIRTPCGHVYHESCIFR 207

BLAST of Spg020133 vs. NCBI nr
Match: KAG6571005.1 (E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 100.5 bits (249), Expect = 2.3e-17
Identity = 58/138 (42.03%), Postives = 80/138 (57.97%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHS--W-MRLIPV---------RYAV 210
           A +VI+  +A + +Q+  E+ + N H+VA VD +  S  W ++ +PV         R  V
Sbjct: 89  ASEVISAQIASFTLQLAEEHNDTNVHVVAVVDNVMESRFWRLQAVPVVQGGSAAVERREV 148

Query: 211 IVEDAR---RPRMEKL----EEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCIL 270
           + E A    R RMEK+    EE  EDLG CSICLDE K     +V++ PC HVY   CI 
Sbjct: 149 VEEVAAANGRYRMEKVEAEEEEEEEDLGNCSICLDEMKCEG-SEVIRTPCGHVYHESCIF 208

BLAST of Spg020133 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.9e-10
Identity = 25/55 (45.45%), Postives = 38/55 (69.09%), Query Frame = 0

Query: 217 DLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPLED 272
           ++  C++C+DEF+  +  DV ++PC HV+   C+L WLE  NSCP+CR  LP +D
Sbjct: 212 EMNQCAVCMDEFEDGS--DVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDD 264

BLAST of Spg020133 vs. ExPASy Swiss-Prot
Match: Q9CY62 (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.1e-09
Identity = 37/99 (37.37%), Postives = 51/99 (51.52%), Query Frame = 0

Query: 173 MNFHLVATVDQIHHSWMRLIPVRYAVIVEDARRPRMEKLEEVGEDLGLCSICLDEFKSNN 232
           M+F  +  VD  HH    L P     +VE   R     +     DL  C +CL EF++  
Sbjct: 48  MDFEDLGLVDWEHH----LPPPAAKAVVESLPR---TVISSAKADL-KCPVCLLEFEAEE 107

Query: 233 KGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPLED 272
              V+++PC H++ + CIL WL   NSCPLCR  LP +D
Sbjct: 108 --TVIEMPCHHLFHSNCILPWLSKTNSCPLCRHELPTDD 136

BLAST of Spg020133 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 5.4e-09
Identity = 38/101 (37.62%), Postives = 50/101 (49.50%), Query Frame = 0

Query: 173 MNFHLVATVDQIHHSWMRLIPVRYAVIVEDARRP--RMEKLEEVGEDLGLCSICLDEFKS 232
           M+F  +  VD  HH    L P     +VE   R   R  K E        C +CL EF+ 
Sbjct: 48  MDFEDLGLVDWEHH----LPPPAAKAVVESLPRTVIRSSKAEL------KCPVCLLEFEE 107

Query: 233 NNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPLED 272
                V+++PC H++ + CIL WL   NSCPLCR  LP +D
Sbjct: 108 EE--TVIEMPCHHLFHSNCILPWLSKTNSCPLCRHELPTDD 136

BLAST of Spg020133 vs. ExPASy Swiss-Prot
Match: Q9Y7K6 (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC2A9.04c PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 7.0e-09
Identity = 22/50 (44.00%), Postives = 31/50 (62.00%), Query Frame = 0

Query: 221 CSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPLE 271
           C IC D+   N++    K+PC H++   C+ KWLEN  +CPLCR  +P E
Sbjct: 107 CPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRKEVPHE 156

BLAST of Spg020133 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 9.2e-09
Identity = 22/58 (37.93%), Postives = 36/58 (62.07%), Query Frame = 0

Query: 214 VGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPLED 272
           +  +   C++C+D+F+     +  ++PC H+Y   C+L WLE  NSCP+CR  LP +D
Sbjct: 217 LNSEFNQCAVCMDDFEEGT--EAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDD 272

BLAST of Spg020133 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 7.8e-19
Identity = 57/137 (41.61%), Postives = 76/137 (55.47%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHSWMRL------IPVRYAVIVEDA- 210
           A + +   +A +++QM   N + NFH++A VD I   W+ L            V+ ++A 
Sbjct: 88  ASQSMARQIASHILQMPDRNGDRNFHIIAEVDFIRVIWLELEEQSMGAGAEETVVFDEAP 147

Query: 211 ---------RRPRMEKLEEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWL 270
                     R    K EE  EDLG CSICLDE  S  K +V++IPC HVY   CI KWL
Sbjct: 148 PALKRGVGKARGERLKSEEKVEDLGDCSICLDEL-SCEKREVIRIPCGHVYHESCIFKWL 207

Query: 271 ENKNSCPLCRSPLPLED 272
           +N NSCPLCR PLPL++
Sbjct: 208 KNHNSCPLCRKPLPLKE 223

BLAST of Spg020133 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 7.8e-19
Identity = 57/137 (41.61%), Postives = 76/137 (55.47%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHSWMRL------IPVRYAVIVEDA- 210
           A + +   +A +++QM   N + NFH++A VD I   W+ L            V+ ++A 
Sbjct: 88  ASQSMARQIASHILQMPDRNGDRNFHIIAEVDFIRVIWLELEEQSMGAGAEETVVFDEAP 147

Query: 211 ---------RRPRMEKLEEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWL 270
                     R    K EE  EDLG CSICLDE  S  K +V++IPC HVY   CI KWL
Sbjct: 148 PALKRGVGKARGERLKSEEKVEDLGDCSICLDEL-SCEKREVIRIPCGHVYHESCIFKWL 207

Query: 271 ENKNSCPLCRSPLPLED 272
           +N NSCPLCR PLPL++
Sbjct: 208 KNHNSCPLCRKPLPLKE 223

BLAST of Spg020133 vs. ExPASy TrEMBL
Match: A0A6J1FXN0 (RING-H2 finger protein ATL16-like OS=Cucurbita moschata OX=3662 GN=LOC111448613 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 8.6e-18
Identity = 58/137 (42.34%), Postives = 80/137 (58.39%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHS--W-MRLIPV---------RYAV 210
           A +VI+  +A + +Q+  E+ + N H+VA VD +  S  W ++ +PV         R  V
Sbjct: 88  ASEVISAQIASFTLQLAEEHNDTNVHVVAVVDNVMESRFWRLQAVPVVQGGSAAVERREV 147

Query: 211 IVEDAR---RPRMEKL---EEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILK 270
           + E A    R RMEK+   EE  EDLG CSICLDE K     +V++ PC HVY   CI +
Sbjct: 148 VEEVAAANGRYRMEKVEAEEEEEEDLGNCSICLDEMKCEG-SEVIRTPCGHVYHESCIFR 207

BLAST of Spg020133 vs. ExPASy TrEMBL
Match: A0A6J1J8T7 (RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111483640 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.5e-17
Identity = 58/137 (42.34%), Postives = 79/137 (57.66%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHS--W-MRLIPV---------RYAV 210
           A +VI   +A + +Q+  E+ + N H+VA VD +  S  W ++ +PV         R  V
Sbjct: 89  ASEVIAAQIASFTLQLAEEHNDTNVHVVAVVDNVVESRFWRLQAVPVMQGGRAAEERREV 148

Query: 211 IVEDAR---RPRMEKLEEVGE---DLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILK 270
           + E A    R RMEK+EE  E   DLG CSICLDE K     +V++ PC HVY   CI +
Sbjct: 149 VEEVAAANGRYRMEKVEEKEEEEVDLGNCSICLDEMKCEG-SEVIRTPCGHVYHESCIFR 208

BLAST of Spg020133 vs. ExPASy TrEMBL
Match: A0A6J1EKL4 (E3 ubiquitin-protein ligase arkadia-like OS=Cucurbita moschata OX=3662 GN=LOC111435522 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.3e-17
Identity = 58/142 (40.85%), Postives = 79/142 (55.63%), Query Frame = 0

Query: 151 ACKVITGHVALYVVQMVAENREMNFHLVATVDQIHHSWMRLIP------------VRYAV 210
           AC+VI   +A  + Q+ A NRE +FH+V  VD I + WM  +             V   V
Sbjct: 61  ACEVIGWKIASQIAQLSAGNRETSFHIVVEVDFIQNIWMGQVAGMRQGQGNGGGLVEETV 120

Query: 211 IVED--ARR-------PRMEKLEEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPC 270
           ++++  ARR        R+E  EE  ++LG C ICLDE   N K +V++ PC HVY   C
Sbjct: 121 VLDELPARRRQRRGVVARVEVEEE--KELGDCCICLDEM-MNKKREVIRTPCGHVYHESC 180

Query: 271 ILKWLENKNSCPLCRSPLPLED 272
           I +WL   NSCPLC+ PLPLE+
Sbjct: 181 IFQWLNLHNSCPLCKRPLPLEE 199

BLAST of Spg020133 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 3.4e-11
Identity = 25/55 (45.45%), Postives = 38/55 (69.09%), Query Frame = 0

Query: 217 DLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPLED 272
           ++  C++C+DEF+  +  DV ++PC HV+   C+L WLE  NSCP+CR  LP +D
Sbjct: 212 EMNQCAVCMDEFEDGS--DVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDD 264

BLAST of Spg020133 vs. TAIR 10
Match: AT1G18770.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-10
Identity = 31/78 (39.74%), Postives = 41/78 (52.56%), Query Frame = 0

Query: 193 PVRYAVIVEDARRPRMEKLEEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILK 252
           P    V+   AR+         GE   +C ICL+EF    +  VV +PC H +D  C+LK
Sbjct: 34  PASKLVVKSLARKIYKMTTSSTGE---MCIICLEEFSEGRR--VVTLPCGHDFDDECVLK 93

Query: 253 WLENKNSCPLCRSPLPLE 271
           W E  +SCPLCR  LP +
Sbjct: 94  WFETNHSCPLCRFKLPYQ 106

BLAST of Spg020133 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-10
Identity = 24/58 (41.38%), Postives = 36/58 (62.07%), Query Frame = 0

Query: 212 EEVGEDLGLCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPL 270
           + +  D+  C++C++EF     GD  ++PC H+Y   CI+ WL   NSCP+CR  LPL
Sbjct: 215 QHLTNDMSQCTVCMEEFIVG--GDATELPCKHIYHKDCIVPWLRLNNSCPICRRDLPL 270

BLAST of Spg020133 vs. TAIR 10
Match: AT1G18760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.7e-10
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 0

Query: 221 CSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLPLED 272
           C+ICL+EF    K  V+ +PC H +D  C+L W E  + CPLCR  LP ED
Sbjct: 175 CTICLEEFNDGTK--VMTLPCGHEFDDECVLTWFETNHDCPLCRFKLPCED 223

BLAST of Spg020133 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.9e-10
Identity = 27/63 (42.86%), Postives = 41/63 (65.08%), Query Frame = 0

Query: 211 LEEVGEDLG--LCSICLDEFKSNNKGDVVKIPCDHVYDAPCILKWLENKNSCPLCRSPLP 270
           LE   +D G  +C++C DE    NK   V++PC+H Y + CI+ WL+ +N+CP+CR  LP
Sbjct: 297 LEGENDDDGGLVCAVCKDEMNIGNK--AVQLPCNHKYHSECIVPWLKVRNTCPVCRYELP 356

Query: 271 LED 272
            +D
Sbjct: 357 TDD 357