Spg020061 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg020061 vs. NCBI nr
Match: XP_038902824.1 (uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902826.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902827.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902828.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902829.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902830.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902831.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902832.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902833.1 uncharacterized protein LOC120089436 [Benincasa hispida] >XP_038902834.1 uncharacterized protein LOC120089436 [Benincasa hispida])

HSP 1 Score: 1227.6 bits (3175), Expect = 0.0e+00
Identity = 656/911 (72.01%), Postives = 739/911 (81.12%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH Q RQ SNLQFN+NVPGCFWSIF+TIDYH WHNVKKMLPYRKHSRSKGG K T
Sbjct: 1   MFKMEKHIQ-RQDSNLQFNKNVPGCFWSIFNTIDYHGWHNVKKMLPYRKHSRSKGGFKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           LN+HHVAEVPE INDEN+ L+CT+ESCP DR   E +V+EV+T  +S EE Q+YWKL+SN
Sbjct: 61  LNSHHVAEVPELINDENESLMCTAESCPIDRASREAHVNEVITNELSGEEGQKYWKLSSN 120

Query: 121 SKRRLSRTHSIHHLEPSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDY-IQRK 180
           SKRR SRT SIHHLEPSY+SP  NG+  DS+  P +  A  + S+SLNAMD+EDY IQR+
Sbjct: 121 SKRRFSRTQSIHHLEPSYHSPVYNGEKDDSQKPPMKLAASGVSSNSLNAMDSEDYLIQRQ 180

Query: 181 IAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHGN 240
           IAIR TS TEKSNGVKK LETN++ +NISS S+KED+HIQEIF+ANRKLFAELLQGAH  
Sbjct: 181 IAIRFTSLTEKSNGVKKTLETNEIIRNISSRSYKEDTHIQEIFKANRKLFAELLQGAHST 240

Query: 241 HTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSKL 300
           +TLQ  +NKKS ASLAKS SFPAPG+ARKGYKKLSSLQHKQ +SFPK QKSVS PQPSKL
Sbjct: 241 NTLQTSQNKKSPASLAKSMSFPAPGLARKGYKKLSSLQHKQIESFPKVQKSVS-PQPSKL 300

Query: 301 VESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQK 360
           VES SPKNFHEDM PCDSDSTS H+IRQQTTSSSLG NRGLR+GGWNQLV+KRFNFIKQK
Sbjct: 301 VESVSPKNFHEDMTPCDSDSTSNHDIRQQTTSSSLGSNRGLRHGGWNQLVVKRFNFIKQK 360

Query: 361 IRHSFKERKKGNNQKTAKGIPTADPSGHELPLDREEAQESLEITAREDGSGIR------- 420
           I+HSFKERKKGNNQKT+KGI T DPSG EL L REEA ESL  T  EDGSG R       
Sbjct: 361 IKHSFKERKKGNNQKTSKGISTLDPSGPELSLYREEAHESLGTTTNEDGSGTRGYSEISH 420

Query: 421 ------------------------------------GYSETDRSENENLVNGVQTKTGTT 480
                                               GYSETDR+EN+NL + VQTKT T 
Sbjct: 421 SVTDYLSNGGQTKIGIDSLSASRERCPQLSVGSGIIGYSETDRTENDNLSSRVQTKTRTA 480

Query: 481 SLNASLERYSQLAENGSNKNREAKCYHSQSLRLKSEDKISNREQPRKSFGRNLSMPDIDL 540
           SL+ASLERYSQL+E G +KNREAK YHS+SLRL SE+K+ N E+P+K FGRNLS PDIDL
Sbjct: 481 SLSASLERYSQLSEYGFDKNREAKGYHSRSLRLISEEKVPNLERPQKRFGRNLSSPDIDL 540

Query: 541 FCTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHLSNADISEALDSHSQSVTEKS 600
           FCTLFTDP H V  TEKPKR LVHS TDNNI+ D+N  H  +  ISE LDS SQ + E+ 
Sbjct: 541 FCTLFTDP-HVVSRTEKPKRGLVHSSTDNNIRTDENSTH--HTQISEPLDSDSQCMMERG 600

Query: 601 DDNIPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNKHQQVLSGSECRVEVVGETVD 660
           +DN+ VDYSGSLNE+  +EG AWAD L+E+I HLDI D KH QV SG+E  VE   ET D
Sbjct: 601 EDNMHVDYSGSLNEITNDEGIAWADVLKERIPHLDILDGKHHQV-SGNERIVEDASETAD 660

Query: 661 DVTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELEPFDDQPSKASIEALP 720
            V++L H+NQVLEL TCF+DDET KFS+S+GAILN GCSIANELEP DDQ ++A  EALP
Sbjct: 661 QVSELSHVNQVLELGTCFQDDETSKFSDSQGAILNPGCSIANELEPSDDQHNEAGTEALP 720

Query: 721 AFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYMRYILQLSSFIESGHTIDWPLSS 780
           AFETIVNHEI DDAEKISN L LHS+ GR+NNADFNYMR+ILQLSSFIE+GHTID PL+S
Sbjct: 721 AFETIVNHEIVDDAEKISNCLYLHSELGRINNADFNYMRHILQLSSFIENGHTIDRPLNS 780

Query: 781 TIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQLR 840
           +IF+GEEAHFYKKLECYW+KVD+DSDHQLLLDLV ETLHN +EKSFI FLKTFS + Q+R
Sbjct: 781 SIFEGEEAHFYKKLECYWDKVDKDSDHQLLLDLVNETLHNVYEKSFICFLKTFSSRSQIR 840

Query: 841 PMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDLSKGDDWMNLQSETEYISLELED 867
           P+ LGQYLLEEVRE++ WYLCLGPELDQSLDDVVGRDL KGDDWMNLQSETE+I+LELED
Sbjct: 841 PVALGQYLLEEVRERVSWYLCLGPELDQSLDDVVGRDLRKGDDWMNLQSETEHIALELED 900

BLAST of Spg020061 vs. NCBI nr
Match: XP_022943817.1 (uncharacterized protein LOC111448443 isoform X4 [Cucurbita moschata])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 646/874 (73.91%), Postives = 721/874 (82.49%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNR+VPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRHVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +NNHHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q YWK+NSN
Sbjct: 61  MNNHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQNYWKINSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  +R
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTRR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTE+SNGV+K LE+ Q+++NI S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEQSNGVRKTLESKQIDRNILSHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSDS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           LVESESPKNFHED+MPCDSDSTS  NI  QT+SSS GPNRGLR+GGWNQLV+KRFNFIKQ
Sbjct: 301 LVESESPKNFHEDVMPCDSDSTSSSNITSQTSSSSFGPNRGLRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL      DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLSREEAQESLGTATSNDGLGIRGYSE 420

Query: 421 TDRSENENLVNGVQTKTGTTSLNASLERYSQLAENGSNKNREAKCYHSQSLRLKSEDKIS 480
           T  SEN+NL +GVQTKTGT SL A LERYSQ  E      REAKC+HSQSLRL  E+ I 
Sbjct: 421 TGNSENDNLSDGVQTKTGTASLKAPLERYSQQPE------REAKCFHSQSLRLIREETIP 480

Query: 481 NREQPRKSFGRNLSMPDIDLFCTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHL 540
           N E+ RKS+GRNLS+PDIDLFCTLFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL
Sbjct: 481 NIEKRRKSYGRNLSLPDIDLFCTLFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHL 540

Query: 541 SNADISEALDSHSQSVTEKSDDNIPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNK 600
            N  ISE LDS SQSV EKSDDN+PVDYSGSLNEV+ +EGAAWAD+LEE+I HLD SD +
Sbjct: 541 LNEVISEPLDSDSQSVVEKSDDNMPVDYSGSLNEVKNDEGAAWADKLEEKIPHLDFSDGE 600

Query: 601 HQQVLSGSECRVE--VVGETVDDVTKLL-HINQVLELETCFEDDETLKFSNSEGAILNLG 660
           H QVL GSEC +E   V ETVD V  L   ++QV   ETC  DDET K S+SEG+ILN  
Sbjct: 601 HHQVL-GSECVIEDVDVSETVDQVGALSPTVDQV--SETCLRDDETSKLSDSEGSILNRR 660

Query: 661 CSIANELEPFDDQPSKASIEALPAFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNY 720
           CS ANELEP DDQP +AS EAL A ETIVNHEI  DAEKISNYL L+S+ GR+NNADFNY
Sbjct: 661 CSAANELEPSDDQPKEASAEALSASETIVNHEII-DAEKISNYLYLNSELGRINNADFNY 720

Query: 721 MRYILQLSSFIESGHTIDWPLSSTIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYET 780
           MRYILQLSSFIESGHTID PLSS+IF+GEEA FYKKLECYWEKVD+DSDHQLL DLVYET
Sbjct: 721 MRYILQLSSFIESGHTIDRPLSSSIFEGEEAQFYKKLECYWEKVDKDSDHQLLHDLVYET 780

Query: 781 LHNAFEKSFIYFLKTFSLKIQLRPMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRD 840
            HN FEKSF  FLKTFS + Q+RPMPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRD
Sbjct: 781 SHNVFEKSFTSFLKTFSSRTQIRPMPLGQYLLEDVREKVAWYLCLGPELDQSLDDVVGRD 840

Query: 841 LSKGDDWMNLQSETEYISLELEDLILDELLDEVL 867
           L KGDDWMNLQ+E EY +LELEDLILDELL+EV+
Sbjct: 841 LRKGDDWMNLQTEIEYNALELEDLILDELLEEVI 853

BLAST of Spg020061 vs. NCBI nr
Match: XP_023511930.1 (uncharacterized protein LOC111776795 [Cucurbita pepo subsp. pepo] >XP_023511931.1 uncharacterized protein LOC111776795 [Cucurbita pepo subsp. pepo] >XP_023511932.1 uncharacterized protein LOC111776795 [Cucurbita pepo subsp. pepo] >XP_023511933.1 uncharacterized protein LOC111776795 [Cucurbita pepo subsp. pepo] >XP_023511934.1 uncharacterized protein LOC111776795 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1200.3 bits (3104), Expect = 0.0e+00
Identity = 642/873 (73.54%), Postives = 719/873 (82.36%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +N+HHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q+YWKLNSN
Sbjct: 61  MNSHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQKYWKLNSN 120

Query: 121 SKRRLSRTHSIHHLEPSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QRK 180
           SKR LSRT SIHHLE S Y            I+P +  A  +KS+SLNAMD+E+Y  +RK
Sbjct: 121 SKRSLSRTQSIHHLESSSY--------YCRHISPMELGAPGIKSNSLNAMDSENYFTRRK 180

Query: 181 IAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHGN 240
           I  + TSFTEKSNGV+K LE+ Q+++NISS SFKED HIQEIF+ANRKLFAELLQGA   
Sbjct: 181 IDTQLTSFTEKSNGVRKTLESKQIDRNISSHSFKEDVHIQEIFKANRKLFAELLQGAQSK 240

Query: 241 HTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSKL 300
            TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHK N+SFPK +KS S  QPSKL
Sbjct: 241 KTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKLNESFPKVRKSGS-SQPSKL 300

Query: 301 VESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQK 360
           VESESPKNFHED+MPCDSDSTS  NI  QT+SSS GPNRGLR+GGWNQLV+KRFNFIKQK
Sbjct: 301 VESESPKNFHEDVMPCDSDSTSSSNITPQTSSSSFGPNRGLRHGGWNQLVVKRFNFIKQK 360

Query: 361 IRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSET 420
           IRHSFKERKKGNNQKT+KG P  T +PSGHELPL REEAQESL      DG GIRGYSET
Sbjct: 361 IRHSFKERKKGNNQKTSKGTPTQTVNPSGHELPLSREEAQESLGTATSNDGLGIRGYSET 420

Query: 421 DRSENENLVNGVQTKTGTTSLNASLERYSQLAENGSNKNREAKCYHSQSLRLKSEDKISN 480
             SEN+NL +GVQTKTGT SL A LERYSQ  E      REAKC+ SQSLRL  E+ I N
Sbjct: 421 GNSENDNLSDGVQTKTGTASLKAPLERYSQQPE------REAKCFDSQSLRLIREETIPN 480

Query: 481 REQPRKSFGRNLSMPDIDLFCTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHLS 540
            E+ RKS+GRNLS+PDIDLFCTLFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL 
Sbjct: 481 IEKRRKSYGRNLSLPDIDLFCTLFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHLL 540

Query: 541 NADISEALDSHSQSVTEKSDDNIPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNKH 600
           N  ISE LDS SQSV EKSDDN+PVDYSGSLNEV+ +EGAAWAD+LEE+I HLD SD +H
Sbjct: 541 NEVISEPLDSDSQSVVEKSDDNMPVDYSGSLNEVKNDEGAAWADKLEEKIPHLDFSDGEH 600

Query: 601 QQVLSGSECRVE--VVGETVDDVTKLL-HINQVLELETCFEDDETLKFSNSEGAILNLGC 660
            QVL GSEC +E   V +TVD V +L   ++QV   ETCF DDET K S+SEG+ILN  C
Sbjct: 601 HQVL-GSECVIEDVDVSDTVDQVGELSPTVDQV--SETCFRDDETSKLSDSEGSILNRRC 660

Query: 661 SIANELEPFDDQPSKASIEALPAFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYM 720
           S ANELEP DDQP +AS EAL A ETIVNHEI  DAEKISNYL L+S+ GR+NNADFNYM
Sbjct: 661 SAANELEPSDDQPKEASAEALSASETIVNHEII-DAEKISNYLYLNSELGRINNADFNYM 720

Query: 721 RYILQLSSFIESGHTIDWPLSSTIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETL 780
           RYILQLSSFIESGHTID PLSS+IF+GEEA FYKKLECYWEKVD+DSDHQLL DLVYET 
Sbjct: 721 RYILQLSSFIESGHTIDRPLSSSIFEGEEAQFYKKLECYWEKVDKDSDHQLLHDLVYETS 780

Query: 781 HNAFEKSFIYFLKTFSLKIQLRPMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDL 840
           HN FEKSF  FLK FS + Q+RPMPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRDL
Sbjct: 781 HNVFEKSFTSFLKNFSSRSQIRPMPLGQYLLEDVREKVAWYLCLGPELDQSLDDVVGRDL 840

Query: 841 SKGDDWMNLQSETEYISLELEDLILDELLDEVL 867
            KGDDWMNLQ+E EY +LELEDLILDELL+EV+
Sbjct: 841 RKGDDWMNLQTEIEYNALELEDLILDELLEEVI 853

BLAST of Spg020061 vs. NCBI nr
Match: XP_022943805.1 (uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943806.1 uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943808.1 uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943809.1 uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943810.1 uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943811.1 uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943812.1 uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943814.1 uncharacterized protein LOC111448443 isoform X1 [Cucurbita moschata] >XP_022943815.1 uncharacterized protein LOC111448443 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 645/911 (70.80%), Postives = 723/911 (79.36%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNR+VPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRHVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +NNHHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q YWK+NSN
Sbjct: 61  MNNHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQNYWKINSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  +R
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTRR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTE+SNGV+K LE+ Q+++NI S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEQSNGVRKTLESKQIDRNILSHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSDS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           LVESESPKNFHED+MPCDSDSTS  NI  QT+SSS GPNRGLR+GGWNQLV+KRFNFIKQ
Sbjct: 301 LVESESPKNFHEDVMPCDSDSTSSSNITSQTSSSSFGPNRGLRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL      DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLSREEAQESLGTATSNDGLGIRGYSE 420

Query: 421 TDRSENENLVNGVQTKTGTTSLNASLERYSQLAENGS----------------------- 480
           T  SEN+NL +GVQTKTGT    ASLERYS+L++  S                       
Sbjct: 421 TGNSENDNLSDGVQTKTGT----ASLERYSKLSDGSSVRGYSVAYSSENVYLSIGVQTKT 480

Query: 481 --------------NKNREAKCYHSQSLRLKSEDKISNREQPRKSFGRNLSMPDIDLFCT 540
                            REAKC+HSQSLRL  E+ I N E+ RKS+GRNLS+PDIDLFCT
Sbjct: 481 GTASLKAPLERYSQQPEREAKCFHSQSLRLIREETIPNIEKRRKSYGRNLSLPDIDLFCT 540

Query: 541 LFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHLSNADISEALDSHSQSVTEKSDDN 600
           LFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL N  ISE LDS SQSV EKSDDN
Sbjct: 541 LFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHLLNEVISEPLDSDSQSVVEKSDDN 600

Query: 601 IPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNKHQQVLSGSECRVE--VVGETVDD 660
           +PVDYSGSLNEV+ +EGAAWAD+LEE+I HLD SD +H QVL GSEC +E   V ETVD 
Sbjct: 601 MPVDYSGSLNEVKNDEGAAWADKLEEKIPHLDFSDGEHHQVL-GSECVIEDVDVSETVDQ 660

Query: 661 VTKLL-HINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELEPFDDQPSKASIEALP 720
           V  L   ++QV   ETC  DDET K S+SEG+ILN  CS ANELEP DDQP +AS EAL 
Sbjct: 661 VGALSPTVDQV--SETCLRDDETSKLSDSEGSILNRRCSAANELEPSDDQPKEASAEALS 720

Query: 721 AFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYMRYILQLSSFIESGHTIDWPLSS 780
           A ETIVNHEI  DAEKISNYL L+S+ GR+NNADFNYMRYILQLSSFIESGHTID PLSS
Sbjct: 721 ASETIVNHEII-DAEKISNYLYLNSELGRINNADFNYMRYILQLSSFIESGHTIDRPLSS 780

Query: 781 TIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQLR 840
           +IF+GEEA FYKKLECYWEKVD+DSDHQLL DLVYET HN FEKSF  FLKTFS + Q+R
Sbjct: 781 SIFEGEEAQFYKKLECYWEKVDKDSDHQLLHDLVYETSHNVFEKSFTSFLKTFSSRTQIR 840

Query: 841 PMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDLSKGDDWMNLQSETEYISLELED 867
           PMPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRDL KGDDWMNLQ+E EY +LELED
Sbjct: 841 PMPLGQYLLEDVREKVAWYLCLGPELDQSLDDVVGRDLRKGDDWMNLQTEIEYNALELED 892

BLAST of Spg020061 vs. NCBI nr
Match: XP_022943816.1 (uncharacterized protein LOC111448443 isoform X3 [Cucurbita moschata])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 642/910 (70.55%), Postives = 718/910 (78.90%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNR+VPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRHVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +NNHHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q YWK+NSN
Sbjct: 61  MNNHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQNYWKINSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  +R
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTRR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTE+SNGV+K LE+ Q+++NI S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEQSNGVRKTLESKQIDRNILSHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSDS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           LVESESPKNFHED+MPCDSDSTS  NI  QT+SSS GPNRGLR+GGWNQLV+KRFNFIKQ
Sbjct: 301 LVESESPKNFHEDVMPCDSDSTSSSNITSQTSSSSFGPNRGLRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL      DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLSREEAQESLGTATSNDGLGIRGYSE 420

Query: 421 TDRSENENLVNGVQTKTGTTSLNASLERYSQLAENGS----------------------- 480
           T  SEN+NL +GVQTKTGT    ASLERYS+L++  S                       
Sbjct: 421 TGNSENDNLSDGVQTKTGT----ASLERYSKLSDGSSVRGYSVAYSSENVYLSIGVQTKT 480

Query: 481 --------------NKNREAKCYHSQSLRLKSEDKISNREQPRKSFGRNLSMPDIDLFCT 540
                            REAKC+HSQSLRL  E+ I N E+ RKS+GRNLS+PDIDLFCT
Sbjct: 481 GTASLKAPLERYSQQPEREAKCFHSQSLRLIREETIPNIEKRRKSYGRNLSLPDIDLFCT 540

Query: 541 LFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHLSNADISEALDSHSQSVTEKSDDN 600
           LFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL N  ISE LDS SQSV EKSDDN
Sbjct: 541 LFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHLLNEVISEPLDSDSQSVVEKSDDN 600

Query: 601 IPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNKHQQVLSGSECRVE--VVGETVDD 660
           +PVDYSGSLNEV+ +EGAAWAD+LEE+I HLD SD +H QVL GSEC +E   V ETVD 
Sbjct: 601 MPVDYSGSLNEVKNDEGAAWADKLEEKIPHLDFSDGEHHQVL-GSECVIEDVDVSETVDQ 660

Query: 661 VTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELEPFDDQPSKASIEALPA 720
           V            ETC  DDET K S+SEG+ILN  CS ANELEP DDQP +AS EAL A
Sbjct: 661 VG-----------ETCLRDDETSKLSDSEGSILNRRCSAANELEPSDDQPKEASAEALSA 720

Query: 721 FETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYMRYILQLSSFIESGHTIDWPLSST 780
            ETIVNHEI  DAEKISNYL L+S+ GR+NNADFNYMRYILQLSSFIESGHTID PLSS+
Sbjct: 721 SETIVNHEII-DAEKISNYLYLNSELGRINNADFNYMRYILQLSSFIESGHTIDRPLSSS 780

Query: 781 IFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQLRP 840
           IF+GEEA FYKKLECYWEKVD+DSDHQLL DLVYET HN FEKSF  FLKTFS + Q+RP
Sbjct: 781 IFEGEEAQFYKKLECYWEKVDKDSDHQLLHDLVYETSHNVFEKSFTSFLKTFSSRTQIRP 840

Query: 841 MPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDLSKGDDWMNLQSETEYISLELEDL 867
           MPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRDL KGDDWMNLQ+E EY +LELEDL
Sbjct: 841 MPLGQYLLEDVREKVAWYLCLGPELDQSLDDVVGRDLRKGDDWMNLQTEIEYNALELEDL 882

BLAST of Spg020061 vs. ExPASy Swiss-Prot
Match: F4HSD5 (Protein TRM32 OS=Arabidopsis thaliana OX=3702 GN=TRM32 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 7.7e-17
Identity = 98/351 (27.92%), Postives = 163/351 (46.44%), Query Frame = 0

Query: 549 QSVTEKSDDNIPVDYSGSLNEVEKNEGAAWADELEEQILHL-------DISDNKHQQVLS 608
           + V + + D+    YS  L ++ + EG     +L  + L L       D  DNK Q    
Sbjct: 225 KKVLDVAKDDYVGRYSQLLKQISRREGG----DLRSKSLKLSYEEKKSDSRDNKPQFFRR 284

Query: 609 -GSECRVEVVGETVDDVTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELE 668
             S   +EV+G  + D+ +    +     ET   +D+   F   +  + +     A + E
Sbjct: 285 ISSLSSLEVLGSFLTDLPR---DSSTSNQETRISEDQDTNFGAKKSVLSSESPVRAEKEE 344

Query: 669 PFDDQPSKASIEALPAFETIVNHEIFDD-------AEKIS---------NYLLLHSKSGR 728
            ++ Q  ++    L +    +  +  D        AEK           + L ++     
Sbjct: 345 KYEVQEERSQENHLDSSNQRILQQEPDSVPSTNKTAEKTETLLPQGLGLSSLEIYKHEEE 404

Query: 729 LNNADFNYMRYILQLSSFIES------GHTIDWPLSSTIFDGEEAHFYKKLECYWEKVDE 788
             +A F Y++ +L++S F+E+       ++ + PL+ ++            E   ++ + 
Sbjct: 405 DEDAYFCYVKKVLKVSGFLENKDNEEKWYSEEQPLNPSLL----------YELDIQEEET 464

Query: 789 DSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQLRPMPLGQYLLEEVREKIVWYLC-L 848
            +D +LL DLV E +      S IYF KTF         P G+  L+EV  ++ W L  L
Sbjct: 465 VNDKELLFDLVNEAIVETQNHSQIYFPKTF---------PYGKRYLDEVWGRVEWSLSGL 524

Query: 849 GPE-LDQSLDDVVGRD-LSKGDDWMNLQSETEYISLELEDLILDELLDEVL 867
           G E  D+SLDD+VGRD L+K D WMNLQ E+E+++LELEDLI D++LDE+L
Sbjct: 525 GAENRDRSLDDIVGRDLLTKSDGWMNLQGESEWLTLELEDLIFDDVLDELL 549

BLAST of Spg020061 vs. ExPASy TrEMBL
Match: A0A6J1FU32 (uncharacterized protein LOC111448443 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111448443 PE=4 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 646/874 (73.91%), Postives = 721/874 (82.49%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNR+VPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRHVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +NNHHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q YWK+NSN
Sbjct: 61  MNNHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQNYWKINSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  +R
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTRR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTE+SNGV+K LE+ Q+++NI S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEQSNGVRKTLESKQIDRNILSHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSDS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           LVESESPKNFHED+MPCDSDSTS  NI  QT+SSS GPNRGLR+GGWNQLV+KRFNFIKQ
Sbjct: 301 LVESESPKNFHEDVMPCDSDSTSSSNITSQTSSSSFGPNRGLRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL      DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLSREEAQESLGTATSNDGLGIRGYSE 420

Query: 421 TDRSENENLVNGVQTKTGTTSLNASLERYSQLAENGSNKNREAKCYHSQSLRLKSEDKIS 480
           T  SEN+NL +GVQTKTGT SL A LERYSQ  E      REAKC+HSQSLRL  E+ I 
Sbjct: 421 TGNSENDNLSDGVQTKTGTASLKAPLERYSQQPE------REAKCFHSQSLRLIREETIP 480

Query: 481 NREQPRKSFGRNLSMPDIDLFCTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHL 540
           N E+ RKS+GRNLS+PDIDLFCTLFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL
Sbjct: 481 NIEKRRKSYGRNLSLPDIDLFCTLFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHL 540

Query: 541 SNADISEALDSHSQSVTEKSDDNIPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNK 600
            N  ISE LDS SQSV EKSDDN+PVDYSGSLNEV+ +EGAAWAD+LEE+I HLD SD +
Sbjct: 541 LNEVISEPLDSDSQSVVEKSDDNMPVDYSGSLNEVKNDEGAAWADKLEEKIPHLDFSDGE 600

Query: 601 HQQVLSGSECRVE--VVGETVDDVTKLL-HINQVLELETCFEDDETLKFSNSEGAILNLG 660
           H QVL GSEC +E   V ETVD V  L   ++QV   ETC  DDET K S+SEG+ILN  
Sbjct: 601 HHQVL-GSECVIEDVDVSETVDQVGALSPTVDQV--SETCLRDDETSKLSDSEGSILNRR 660

Query: 661 CSIANELEPFDDQPSKASIEALPAFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNY 720
           CS ANELEP DDQP +AS EAL A ETIVNHEI  DAEKISNYL L+S+ GR+NNADFNY
Sbjct: 661 CSAANELEPSDDQPKEASAEALSASETIVNHEII-DAEKISNYLYLNSELGRINNADFNY 720

Query: 721 MRYILQLSSFIESGHTIDWPLSSTIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYET 780
           MRYILQLSSFIESGHTID PLSS+IF+GEEA FYKKLECYWEKVD+DSDHQLL DLVYET
Sbjct: 721 MRYILQLSSFIESGHTIDRPLSSSIFEGEEAQFYKKLECYWEKVDKDSDHQLLHDLVYET 780

Query: 781 LHNAFEKSFIYFLKTFSLKIQLRPMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRD 840
            HN FEKSF  FLKTFS + Q+RPMPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRD
Sbjct: 781 SHNVFEKSFTSFLKTFSSRTQIRPMPLGQYLLEDVREKVAWYLCLGPELDQSLDDVVGRD 840

Query: 841 LSKGDDWMNLQSETEYISLELEDLILDELLDEVL 867
           L KGDDWMNLQ+E EY +LELEDLILDELL+EV+
Sbjct: 841 LRKGDDWMNLQTEIEYNALELEDLILDELLEEVI 853

BLAST of Spg020061 vs. ExPASy TrEMBL
Match: A0A6J1FYA4 (uncharacterized protein LOC111448443 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448443 PE=4 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 645/911 (70.80%), Postives = 723/911 (79.36%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNR+VPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRHVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +NNHHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q YWK+NSN
Sbjct: 61  MNNHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQNYWKINSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  +R
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTRR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTE+SNGV+K LE+ Q+++NI S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEQSNGVRKTLESKQIDRNILSHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSDS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           LVESESPKNFHED+MPCDSDSTS  NI  QT+SSS GPNRGLR+GGWNQLV+KRFNFIKQ
Sbjct: 301 LVESESPKNFHEDVMPCDSDSTSSSNITSQTSSSSFGPNRGLRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL      DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLSREEAQESLGTATSNDGLGIRGYSE 420

Query: 421 TDRSENENLVNGVQTKTGTTSLNASLERYSQLAENGS----------------------- 480
           T  SEN+NL +GVQTKTGT    ASLERYS+L++  S                       
Sbjct: 421 TGNSENDNLSDGVQTKTGT----ASLERYSKLSDGSSVRGYSVAYSSENVYLSIGVQTKT 480

Query: 481 --------------NKNREAKCYHSQSLRLKSEDKISNREQPRKSFGRNLSMPDIDLFCT 540
                            REAKC+HSQSLRL  E+ I N E+ RKS+GRNLS+PDIDLFCT
Sbjct: 481 GTASLKAPLERYSQQPEREAKCFHSQSLRLIREETIPNIEKRRKSYGRNLSLPDIDLFCT 540

Query: 541 LFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHLSNADISEALDSHSQSVTEKSDDN 600
           LFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL N  ISE LDS SQSV EKSDDN
Sbjct: 541 LFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHLLNEVISEPLDSDSQSVVEKSDDN 600

Query: 601 IPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNKHQQVLSGSECRVE--VVGETVDD 660
           +PVDYSGSLNEV+ +EGAAWAD+LEE+I HLD SD +H QVL GSEC +E   V ETVD 
Sbjct: 601 MPVDYSGSLNEVKNDEGAAWADKLEEKIPHLDFSDGEHHQVL-GSECVIEDVDVSETVDQ 660

Query: 661 VTKLL-HINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELEPFDDQPSKASIEALP 720
           V  L   ++QV   ETC  DDET K S+SEG+ILN  CS ANELEP DDQP +AS EAL 
Sbjct: 661 VGALSPTVDQV--SETCLRDDETSKLSDSEGSILNRRCSAANELEPSDDQPKEASAEALS 720

Query: 721 AFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYMRYILQLSSFIESGHTIDWPLSS 780
           A ETIVNHEI  DAEKISNYL L+S+ GR+NNADFNYMRYILQLSSFIESGHTID PLSS
Sbjct: 721 ASETIVNHEII-DAEKISNYLYLNSELGRINNADFNYMRYILQLSSFIESGHTIDRPLSS 780

Query: 781 TIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQLR 840
           +IF+GEEA FYKKLECYWEKVD+DSDHQLL DLVYET HN FEKSF  FLKTFS + Q+R
Sbjct: 781 SIFEGEEAQFYKKLECYWEKVDKDSDHQLLHDLVYETSHNVFEKSFTSFLKTFSSRTQIR 840

Query: 841 PMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDLSKGDDWMNLQSETEYISLELED 867
           PMPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRDL KGDDWMNLQ+E EY +LELED
Sbjct: 841 PMPLGQYLLEDVREKVAWYLCLGPELDQSLDDVVGRDLRKGDDWMNLQTEIEYNALELED 892

BLAST of Spg020061 vs. ExPASy TrEMBL
Match: A0A6J1FSR2 (uncharacterized protein LOC111448443 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111448443 PE=4 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 642/910 (70.55%), Postives = 718/910 (78.90%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNR+VPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRHVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +NNHHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q YWK+NSN
Sbjct: 61  MNNHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQNYWKINSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  +R
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTRR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTE+SNGV+K LE+ Q+++NI S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEQSNGVRKTLESKQIDRNILSHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSDS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           LVESESPKNFHED+MPCDSDSTS  NI  QT+SSS GPNRGLR+GGWNQLV+KRFNFIKQ
Sbjct: 301 LVESESPKNFHEDVMPCDSDSTSSSNITSQTSSSSFGPNRGLRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL      DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLSREEAQESLGTATSNDGLGIRGYSE 420

Query: 421 TDRSENENLVNGVQTKTGTTSLNASLERYSQLAENGS----------------------- 480
           T  SEN+NL +GVQTKTGT    ASLERYS+L++  S                       
Sbjct: 421 TGNSENDNLSDGVQTKTGT----ASLERYSKLSDGSSVRGYSVAYSSENVYLSIGVQTKT 480

Query: 481 --------------NKNREAKCYHSQSLRLKSEDKISNREQPRKSFGRNLSMPDIDLFCT 540
                            REAKC+HSQSLRL  E+ I N E+ RKS+GRNLS+PDIDLFCT
Sbjct: 481 GTASLKAPLERYSQQPEREAKCFHSQSLRLIREETIPNIEKRRKSYGRNLSLPDIDLFCT 540

Query: 541 LFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHLSNADISEALDSHSQSVTEKSDDN 600
           LFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL N  ISE LDS SQSV EKSDDN
Sbjct: 541 LFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHLLNEVISEPLDSDSQSVVEKSDDN 600

Query: 601 IPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNKHQQVLSGSECRVE--VVGETVDD 660
           +PVDYSGSLNEV+ +EGAAWAD+LEE+I HLD SD +H QVL GSEC +E   V ETVD 
Sbjct: 601 MPVDYSGSLNEVKNDEGAAWADKLEEKIPHLDFSDGEHHQVL-GSECVIEDVDVSETVDQ 660

Query: 661 VTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELEPFDDQPSKASIEALPA 720
           V            ETC  DDET K S+SEG+ILN  CS ANELEP DDQP +AS EAL A
Sbjct: 661 VG-----------ETCLRDDETSKLSDSEGSILNRRCSAANELEPSDDQPKEASAEALSA 720

Query: 721 FETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYMRYILQLSSFIESGHTIDWPLSST 780
            ETIVNHEI  DAEKISNYL L+S+ GR+NNADFNYMRYILQLSSFIESGHTID PLSS+
Sbjct: 721 SETIVNHEII-DAEKISNYLYLNSELGRINNADFNYMRYILQLSSFIESGHTIDRPLSSS 780

Query: 781 IFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQLRP 840
           IF+GEEA FYKKLECYWEKVD+DSDHQLL DLVYET HN FEKSF  FLKTFS + Q+RP
Sbjct: 781 IFEGEEAQFYKKLECYWEKVDKDSDHQLLHDLVYETSHNVFEKSFTSFLKTFSSRTQIRP 840

Query: 841 MPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDLSKGDDWMNLQSETEYISLELEDL 867
           MPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRDL KGDDWMNLQ+E EY +LELEDL
Sbjct: 841 MPLGQYLLEDVREKVAWYLCLGPELDQSLDDVVGRDLRKGDDWMNLQTEIEYNALELEDL 882

BLAST of Spg020061 vs. ExPASy TrEMBL
Match: A0A6J1J8H7 (uncharacterized protein LOC111484426 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484426 PE=4 SV=1)

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 638/873 (73.08%), Postives = 708/873 (81.10%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +N+HHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q+YWKLNSN
Sbjct: 61  MNSHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQKYWKLNSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  QR
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTQR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTEKSNGV+K LE+ Q      S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEKSNGVRKTLESKQ------SHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSGS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           L ESESPKNFHED+MPCDSDSTS  N+  QT+SSSLGPNRG+R+GGWNQLV+KRFNFIKQ
Sbjct: 301 LAESESPKNFHEDVMPCDSDSTSSSNMTPQTSSSSLGPNRGIRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL     +DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLRREEAQESLGTATSDDGLGIRGYSE 420

Query: 421 TDRSENENLVNGVQTKTGTTSLNASLERYSQLAENGSNKNREAKCYHSQSLRLKSEDKIS 480
           T  SEN+NL +GVQTKTGT SL A LERYSQ  E      REAKC+HSQSLRL  E+ I 
Sbjct: 421 TGNSENDNLSDGVQTKTGTASLKAPLERYSQQPE------REAKCFHSQSLRLIREETIP 480

Query: 481 NREQPRKSFGRNLSMPDIDLFCTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHL 540
           N E+ RKS+GRNLS+PDIDLFCTLFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL
Sbjct: 481 NIEKRRKSYGRNLSLPDIDLFCTLFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHL 540

Query: 541 SNADISEALDSHSQSVTEKSDDNIPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNK 600
            N  ISE LDS SQSV EKSDDN+PVDYS  LNEV+ +EGAAWAD LEE+I HLD SD +
Sbjct: 541 LNEVISEPLDSDSQSVVEKSDDNMPVDYSCCLNEVKNDEGAAWADRLEEKIPHLDFSDGE 600

Query: 601 HQQVLSGSECRVE--VVGETVDDVTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGC 660
           H QVL GSEC +E   V ETVD V            ETCF DDET K S+SEG+ILN  C
Sbjct: 601 HHQVL-GSECVIEDVDVSETVDQVG-----------ETCFRDDETSKLSDSEGSILNRRC 660

Query: 661 SIANELEPFDDQPSKASIEALPAFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYM 720
           S+ANELEP DDQP +A  EAL A ET VNHEI  DAEKISNYL L+S  GR+NNADFNYM
Sbjct: 661 SVANELEPSDDQPKEARAEALSASETFVNHEII-DAEKISNYLHLNSDLGRINNADFNYM 720

Query: 721 RYILQLSSFIESGHTIDWPLSSTIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETL 780
           RYILQLSSFIESGHTID PLSS+IF+GEEA FYKKLECYWEKVD+ SDHQLL DLVYET 
Sbjct: 721 RYILQLSSFIESGHTIDRPLSSSIFEGEEAQFYKKLECYWEKVDKGSDHQLLHDLVYETS 780

Query: 781 HNAFEKSFIYFLKTFSLKIQLRPMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDL 840
           HN FEKSF  FLKTFS + Q+RPMPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRDL
Sbjct: 781 HNVFEKSFTSFLKTFSSRSQIRPMPLGQYLLEDVREKVSWYLCLGPELDQSLDDVVGRDL 837

Query: 841 SKGDDWMNLQSETEYISLELEDLILDELLDEVL 867
            KGDDWMNLQ+E EY +LELEDLILDELL+EV+
Sbjct: 841 RKGDDWMNLQTEIEYNALELEDLILDELLEEVI 837

BLAST of Spg020061 vs. ExPASy TrEMBL
Match: A0A6J1JH08 (uncharacterized protein LOC111484426 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484426 PE=4 SV=1)

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 639/912 (70.07%), Postives = 708/912 (77.63%), Query Frame = 0

Query: 1   MFKMEKHAQRRQGSNLQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRKHSRSKGGPKLT 60
           MFKMEKH  +RQ SN+QFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYR+HS+SKG PK T
Sbjct: 1   MFKMEKHI-KRQDSNIQFNRNVPGCFWSIFHTIDYHPWHNVKKMLPYRRHSKSKGSPKST 60

Query: 61  LNNHHVAEVPEQINDENKPLLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 120
           +N+HHVAE  EQINDENKPL CT+ESCP   +PGE  V+EV++K +SEEE Q+YWKLNSN
Sbjct: 61  MNSHHVAEATEQINDENKPLWCTAESCPIGSKPGEGYVNEVISKEISEEESQKYWKLNSN 120

Query: 121 SKRRLSRTHSIHHLE-PSYYSPGNNGDIRDSRITPQQRTAI-MKSSSLNAMDTEDYI-QR 180
           SKR LSRT SIHHLE  SYYS           I+P +  A  +KS+SLNAMD+E+Y  QR
Sbjct: 121 SKRSLSRTQSIHHLESSSYYS---------RHISPMELGAPGIKSNSLNAMDSENYFTQR 180

Query: 181 KIAIRTTSFTEKSNGVKKNLETNQVNKNISSCSFKEDSHIQEIFRANRKLFAELLQGAHG 240
           KI  + TSFTEKSNGV+K LE+ Q      S SFKED HIQEIF+ANRKLFAELLQGA  
Sbjct: 181 KIDTQLTSFTEKSNGVRKTLESKQ------SHSFKEDVHIQEIFKANRKLFAELLQGAQS 240

Query: 241 NHTLQPPKNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSK 300
             TLQ P+NKKSSASLAKSRSFPAPG+ARKGYKKLSSLQHKQN+SFPK +KS S PQPSK
Sbjct: 241 KKTLQTPQNKKSSASLAKSRSFPAPGLARKGYKKLSSLQHKQNESFPKVRKSGS-PQPSK 300

Query: 301 LVESESPKNFHEDMMPCDSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 360
           L ESESPKNFHED+MPCDSDSTS  N+  QT+SSSLGPNRG+R+GGWNQLV+KRFNFIKQ
Sbjct: 301 LAESESPKNFHEDVMPCDSDSTSSSNMTPQTSSSSLGPNRGIRHGGWNQLVVKRFNFIKQ 360

Query: 361 KIRHSFKERKKGNNQKTAKGIP--TADPSGHELPLDREEAQESLEITAREDGSGIRGYSE 420
           KIRHSFKERKKGNNQKT+KG P  T DPSGHELPL REEAQESL     +DG GIRGYSE
Sbjct: 361 KIRHSFKERKKGNNQKTSKGTPTQTVDPSGHELPLRREEAQESLGTATSDDGLGIRGYSE 420

Query: 421 TDR---------------------------------------SENENLVNGVQTKTGTTS 480
           T                                         SEN+NL NGVQTKTGT S
Sbjct: 421 TGNSENDNLSDGVQTKTRTASLERYSKLSDGSGIRGYSEAYSSENDNLSNGVQTKTGTAS 480

Query: 481 LNASLERYSQLAENGSNKNREAKCYHSQSLRLKSEDKISNREQPRKSFGRNLSMPDIDLF 540
           L A LERYSQ  E      REAKC+HSQSLRL  E+ I N E+ RKS+GRNLS+PDIDLF
Sbjct: 481 LKAPLERYSQQPE------REAKCFHSQSLRLIREETIPNIEKRRKSYGRNLSLPDIDLF 540

Query: 541 CTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPAHLSNADISEALDSHSQSVTEKSD 600
           CTLFTDP+  V  TEKPKR +VHS TDNN++ D+NPAHL N  ISE LDS SQSV EKSD
Sbjct: 541 CTLFTDPSPAVSRTEKPKRGVVHSSTDNNMRIDENPAHLLNEVISEPLDSDSQSVVEKSD 600

Query: 601 DNIPVDYSGSLNEVEKNEGAAWADELEEQILHLDISDNKHQQVLSGSECRVE--VVGETV 660
           DN+PVDYS  LNEV+ +EGAAWAD LEE+I HLD SD +H QVL GSEC +E   V ETV
Sbjct: 601 DNMPVDYSCCLNEVKNDEGAAWADRLEEKIPHLDFSDGEHHQVL-GSECVIEDVDVSETV 660

Query: 661 DDVTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELEPFDDQPSKASIEAL 720
           D V            ETCF DDET K S+SEG+ILN  CS+ANELEP DDQP +A  EAL
Sbjct: 661 DQVG-----------ETCFRDDETSKLSDSEGSILNRRCSVANELEPSDDQPKEARAEAL 720

Query: 721 PAFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYMRYILQLSSFIESGHTIDWPLS 780
            A ET VNHEI  DAEKISNYL L+S  GR+NNADFNYMRYILQLSSFIESGHTID PLS
Sbjct: 721 SASETFVNHEII-DAEKISNYLHLNSDLGRINNADFNYMRYILQLSSFIESGHTIDRPLS 780

Query: 781 STIFDGEEAHFYKKLECYWEKVDEDSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQL 840
           S+IF+GEEA FYKKLECYWEKVD+ SDHQLL DLVYET HN FEKSF  FLKTFS + Q+
Sbjct: 781 SSIFEGEEAQFYKKLECYWEKVDKGSDHQLLHDLVYETSHNVFEKSFTSFLKTFSSRSQI 840

Query: 841 RPMPLGQYLLEEVREKIVWYLCLGPELDQSLDDVVGRDLSKGDDWMNLQSETEYISLELE 867
           RPMPLGQYLLE+VREK+ WYLCLGPELDQSLDDVVGRDL KGDDWMNLQ+E EY +LELE
Sbjct: 841 RPMPLGQYLLEDVREKVSWYLCLGPELDQSLDDVVGRDLRKGDDWMNLQTEIEYNALELE 876

BLAST of Spg020061 vs. TAIR 10
Match: AT5G02390.1 (Protein of unknown function (DUF3741) )

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-21
Identity = 214/945 (22.65%), Postives = 366/945 (38.73%), Query Frame = 0

Query: 20  RNVPGCFWSIFHTIDYHPWHNVKKMLPYRKH--SRSKGGPKLTLNNHHVAE-----VP-- 79
           +N PG  W +F  + ++ W  +KK LP+++    R+  G K  +NN   +      VP  
Sbjct: 15  QNHPGFMWGLFDILKHNHWRYIKKRLPHKRPVCRRTAPGTKNEVNNTIPSSPDGIPVPKI 74

Query: 80  --------EQINDENKP--LLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 139
                   E +    KP   + + ES     +  + +  E  +K ++ EER +  K +S 
Sbjct: 75  KLEDKTDVESVQRPKKPSSAVKSKESSNSGEKTKKKHNPEEKSKKLNSEERSR--KTHSE 134

Query: 140 SKRRL------------SRTHSIHHLEPSYYSPGN------------------NGDIRDS 199
            KR +            S+    HH   +Y    N                  NGD R+ 
Sbjct: 135 IKRSVKALIKALVIEDKSKKKGRHHRSCTYPVQSNSKEKDSLSEVGSSDKNSSNGDERNR 194

Query: 200 ------RITPQQRTA----IMKSSSLNAMDTEDYIQRKIAIRTTSFTEKSNGVKKNLETN 259
                  I+P   +     +M   S N+   E  +   I +   +   KS+  +K  +  
Sbjct: 195 VFNKKVGISPAVGSLNPLYLMSEESSNSDSEEFRVDNNIQVDDNTDGSKSDFDEKEFKKK 254

Query: 260 QVNKNISSCSFKEDSHIQ-------EIFRANRKLFAELLQGAHGNH-----TLQPP---- 319
           + + +  +    +  H +       +      K   + L   H N       LQ P    
Sbjct: 255 EKSDDEEAWFDPKMRHTKGFSENDDDTSPRRSKACLDALNLIHMNRNFLLKVLQDPGSPL 314

Query: 320 ------KNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSKL 379
                 +   SS ++ K+ SFP  G+ R+ + K         D  P    S++       
Sbjct: 315 ARHFQSQQSFSSKTMTKAGSFPTHGINREDHNKAFD-PVGDVDKIPMSPPSIAAEH---- 374

Query: 380 VESESPKNFHEDMMPC-DSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 439
             ++  +  +E ++   D DS+     R++               G NQ+VIKRF  ++Q
Sbjct: 375 -RADGIQKMNETVLKLGDEDSSGSGYARKR---------------GKNQVVIKRFKDLRQ 434

Query: 440 KIRHSFKERKKGNNQKTAKGIPTADPSGHELPLD-REEAQESLEITAREDGSGIRGYSET 499
           KI+H   E K   ++ T   +    P  +    D R++  E L+                
Sbjct: 435 KIKHVINENKNEKHRITMDAVLDKVPRKYGFSKDLRDDIFEHLK--------------GN 494

Query: 500 DRSENENLVNGVQTK--TGTTSLNASLERYSQLAENGSNKNREAK-CYHSQSLRLKSEDK 559
             ++N N   G + K  T  +SL  SL+RY QL E   +  REAK   +S+  +++ E+ 
Sbjct: 495 SAAKNNNKAEGTKLKQITRASSLCGSLDRYLQLYE--CSFQREAKNSSNSEKSKMELEES 554

Query: 560 ISNREQPRKSFGRNLSMPDIDLFCTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPA 619
               ++  K  GR LS+P +                  K   + +   T  +I+ +++  
Sbjct: 555 ALPSKRAPKFLGRILSLPQM------------------KFHALKIDDLTVQSIEEEQDGL 614

Query: 620 HLSNADISEALDSHSQSVTEKSDDNIPVDYSGSLNEVEKNEGAAWADELEEQILHLDISD 679
                +ISE  + HSQS   ++ D        S  + E         E + +I  LD+  
Sbjct: 615 D----NISEISEDHSQSSEHETLDQTMDASEDSPVDAE--------TEQDREISTLDVEH 674

Query: 680 NKHQQVLSGSECRVEVVGETVDDVTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGC 739
                  S  E    V    +D+   +  I++ L+ ET                      
Sbjct: 675 ETRSLKESSEESPNNVSTVDIDENASVFDISRDLDTET---------------------V 734

Query: 740 SIANELEPFDDQPSKASIEALPAFETIVNHEIFDDAEKISNYLLLHSKSGRLNNADFNYM 799
           S + +L+                 E ++N    D  +K+                 FNY+
Sbjct: 735 STSKQLD-----------------EEVLN---IDAQDKVK----------------FNYV 794

Query: 800 RYILQLSSFIESGHTIDW-----PLSSTIFD----GEEAHFYKKLECYW-EKVDEDSDHQ 859
           R IL++S F        W     PL   +++          ++  EC   E+   + +H 
Sbjct: 795 RDILEISGFNAPESLSMWQLDYQPLDPLVYEEVTTTTTGCMFQDPECSRNEEEGGNCNHL 833

Query: 860 LLLDLVYETLHNAFEKSFIYFLKTFSLKIQLRPMPLGQYLLEEVREKIVWYLCLGP-ELD 868
           LL DL+ E L   +E+S+ Y+ K  S   ++ PMP+G  +L+EV  +I  YL   P +  
Sbjct: 855 LLFDLINEVLIEIYERSYHYYPKPLSSLCKIHPMPVGYSVLKEVWVRISCYLRYKPYDEQ 833

BLAST of Spg020061 vs. TAIR 10
Match: AT1G07620.1 (GTP-binding protein Obg/CgtA )

HSP 1 Score: 90.9 bits (224), Expect = 5.5e-18
Identity = 98/351 (27.92%), Postives = 163/351 (46.44%), Query Frame = 0

Query: 549 QSVTEKSDDNIPVDYSGSLNEVEKNEGAAWADELEEQILHL-------DISDNKHQQVLS 608
           + V + + D+    YS  L ++ + EG     +L  + L L       D  DNK Q    
Sbjct: 225 KKVLDVAKDDYVGRYSQLLKQISRREGG----DLRSKSLKLSYEEKKSDSRDNKPQFFRR 284

Query: 609 -GSECRVEVVGETVDDVTKLLHINQVLELETCFEDDETLKFSNSEGAILNLGCSIANELE 668
             S   +EV+G  + D+ +    +     ET   +D+   F   +  + +     A + E
Sbjct: 285 ISSLSSLEVLGSFLTDLPR---DSSTSNQETRISEDQDTNFGAKKSVLSSESPVRAEKEE 344

Query: 669 PFDDQPSKASIEALPAFETIVNHEIFDD-------AEKIS---------NYLLLHSKSGR 728
            ++ Q  ++    L +    +  +  D        AEK           + L ++     
Sbjct: 345 KYEVQEERSQENHLDSSNQRILQQEPDSVPSTNKTAEKTETLLPQGLGLSSLEIYKHEEE 404

Query: 729 LNNADFNYMRYILQLSSFIES------GHTIDWPLSSTIFDGEEAHFYKKLECYWEKVDE 788
             +A F Y++ +L++S F+E+       ++ + PL+ ++            E   ++ + 
Sbjct: 405 DEDAYFCYVKKVLKVSGFLENKDNEEKWYSEEQPLNPSLL----------YELDIQEEET 464

Query: 789 DSDHQLLLDLVYETLHNAFEKSFIYFLKTFSLKIQLRPMPLGQYLLEEVREKIVWYLC-L 848
            +D +LL DLV E +      S IYF KTF         P G+  L+EV  ++ W L  L
Sbjct: 465 VNDKELLFDLVNEAIVETQNHSQIYFPKTF---------PYGKRYLDEVWGRVEWSLSGL 524

Query: 849 GPE-LDQSLDDVVGRD-LSKGDDWMNLQSETEYISLELEDLILDELLDEVL 867
           G E  D+SLDD+VGRD L+K D WMNLQ E+E+++LELEDLI D++LDE+L
Sbjct: 525 GAENRDRSLDDIVGRDLLTKSDGWMNLQGESEWLTLELEDLIFDDVLDELL 549

BLAST of Spg020061 vs. TAIR 10
Match: AT5G02390.2 (Protein of unknown function (DUF3741) )

HSP 1 Score: 46.2 bits (108), Expect = 1.5e-04
Identity = 145/699 (20.74%), Postives = 265/699 (37.91%), Query Frame = 0

Query: 20  RNVPGCFWSIFHTIDYHPWHNVKKMLPYRKH--SRSKGGPKLTLNNHHVAE-----VP-- 79
           +N PG  W +F  + ++ W  +KK LP+++    R+  G K  +NN   +      VP  
Sbjct: 15  QNHPGFMWGLFDILKHNHWRYIKKRLPHKRPVCRRTAPGTKNEVNNTIPSSPDGIPVPKI 74

Query: 80  --------EQINDENKP--LLCTSESCPFDRRPGEVNVSEVVTKVMSEEERQQYWKLNSN 139
                   E +    KP   + + ES     +  + +  E  +K ++ EER +  K +S 
Sbjct: 75  KLEDKTDVESVQRPKKPSSAVKSKESSNSGEKTKKKHNPEEKSKKLNSEERSR--KTHSE 134

Query: 140 SKRRL------------SRTHSIHHLEPSYYSPGN------------------NGDIRDS 199
            KR +            S+    HH   +Y    N                  NGD R+ 
Sbjct: 135 IKRSVKALIKALVIEDKSKKKGRHHRSCTYPVQSNSKEKDSLSEVGSSDKNSSNGDERNR 194

Query: 200 ------RITPQQRTA----IMKSSSLNAMDTEDYIQRKIAIRTTSFTEKSNGVKKNLETN 259
                  I+P   +     +M   S N+   E  +   I +   +   KS+  +K  +  
Sbjct: 195 VFNKKVGISPAVGSLNPLYLMSEESSNSDSEEFRVDNNIQVDDNTDGSKSDFDEKEFKKK 254

Query: 260 QVNKNISSCSFKEDSHIQ-------EIFRANRKLFAELLQGAHGNH-----TLQPP---- 319
           + + +  +    +  H +       +      K   + L   H N       LQ P    
Sbjct: 255 EKSDDEEAWFDPKMRHTKGFSENDDDTSPRRSKACLDALNLIHMNRNFLLKVLQDPGSPL 314

Query: 320 ------KNKKSSASLAKSRSFPAPGVARKGYKKLSSLQHKQNDSFPKGQKSVSVPQPSKL 379
                 +   SS ++ K+ SFP  G+ R+ + K         D  P    S++       
Sbjct: 315 ARHFQSQQSFSSKTMTKAGSFPTHGINREDHNKAFD-PVGDVDKIPMSPPSIAAEH---- 374

Query: 380 VESESPKNFHEDMMPC-DSDSTSCHNIRQQTTSSSLGPNRGLRYGGWNQLVIKRFNFIKQ 439
             ++  +  +E ++   D DS+     R++               G NQ+VIKRF  ++Q
Sbjct: 375 -RADGIQKMNETVLKLGDEDSSGSGYARKR---------------GKNQVVIKRFKDLRQ 434

Query: 440 KIRHSFKERKKGNNQKTAKGIPTADPSGHELPLD-REEAQESLEITAREDGSGIRGYSET 499
           KI+H   E K   ++ T   +    P  +    D R++  E L+                
Sbjct: 435 KIKHVINENKNEKHRITMDAVLDKVPRKYGFSKDLRDDIFEHLK--------------GN 494

Query: 500 DRSENENLVNGVQTK--TGTTSLNASLERYSQLAENGSNKNREAK-CYHSQSLRLKSEDK 559
             ++N N   G + K  T  +SL  SL+RY QL E   +  REAK   +S+  +++ E+ 
Sbjct: 495 SAAKNNNKAEGTKLKQITRASSLCGSLDRYLQLYE--CSFQREAKNSSNSEKSKMELEES 554

Query: 560 ISNREQPRKSFGRNLSMPDIDLFCTLFTDPTHGVFGTEKPKRVLVHSRTDNNIQRDKNPA 619
               ++  K  GR LS+P +        D T      E+     +   ++++ Q  ++  
Sbjct: 555 ALPSKRAPKFLGRILSLPQMKFHALKIDDLTVQSIEEEQDGLDNISEISEDHSQSSEHET 614

Query: 620 HLSNADISEALDSHSQSVTEKSDDNIPVDY-SGSLNE-VEKNEGAAWADELEEQILHLDI 630
                D SE     +++  ++    + V++ + SL E  E++       +++E     DI
Sbjct: 615 LDQTMDASEDSPVDAETEQDREISTLDVEHETRSLKESSEESPNNVSTVDIDENASVFDI 674