Spg020028 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg020028
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	DNA-dependent metalloprotease WSS1-like isoform X1
Location	scaffold1: 28213703 .. 28220046 (+)
RNA-Seq Expression	Spg020028
Synteny	Spg020028

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGGGCAACGCATCACACCTCACTCGTTTTGCAACTTTGCCTTCTATAGTCGATAGGGTCTCTTATGCCCATACCTATAACAAAAATACACATGCCTTAGTTTGCTTGGAAATCATGGGGACTGCCACAGATATGCATACATCTGTAGACACATACCCGATACACAAGCATGCATTAGTTTACCTGGAACGCATGGGGAAAGTCGTTAAGACTGTATTTCACTATGTGGTCGCTCTTCTCCTTCACCTTTTTAAACGTGTCCACCATGTCCTTGAGGGCACTTTGCAGCCCCTTGATTGTCCTCTCCCAAATTCAAGTACCCTAGTCAAAGTTGTTGAAGGATGTCAACTACTTGACGTCCCAGAACAATGATTTGTCAACTGATGTCTTTAGTTTGTCCTTCCCCTGTTGGGAGAAAAATGCGGAATACATATAACAATAATATTATAACGACAATCAATAATATTATAACTATCTCCCCATTTACAGGTTAAGACAAGCAAGCCAATAATAATAGTCAAGAAAAATAATAAATGATAGGAAAAAAAAAATGACACCGAAAAATTATACTGGTTCGCCCCAAGCTCGGGACTACGTCCAGTCTTCTGCAACTGCAAGTTCTACTAGAAATTTGGGACATTACAGGAAACATTCAATAACACCACAAATTAACCCTCAATAGTCCAACAATAGTTATTGACTTCCAAGTATCACAACCCGATAACTTGGGATAAACAACGAAAAACACCCTCAACCCAATACAAGAGAATAAATCTTATTCCCTTAAACTCACACAAGAGAAATACTCTTTTCTCTCAAGTTCACTCACTGCCATCTTTGAATTACCGAAGAAAAACACTCCAGTTGCATGAGAGAGCTTTCAGCGGAACAAAGAAAGGTCTCCTCTCTCGGTAAAAAATTCCCCAGTCGCAACAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATAAGTAAAAAACAACCTAATTAAGGCCCAAAACATTCTCCTTTTCTTTTCGTGCACTGCAAGTTGAGGAAGGAGTTTTGTCTCCACACAATCCCATCAATTTTCCACTTGGAGACAAAACAACGATCTTGTCTCCCACTGCAACAAAACTCTAATCATCTTGGCAAACTCCAAGGATTTACTCCCACTACAACTGTTATCTCTGATCTTCTTGGTAAACTCCAAGGCTTAAAATACTCGACAAGCAACAAACTCACAAAAATTGGCAACTATCAAAATATAATTGTAGCTCATCTATGCTTGCTTTGAGGTATGGGTTTGCTACATGGACCTGTCTTCCAGACGAAAAGACAGACACGTATTCCCACTTCAGCTAAGTGGTGACACCTGTCATCTTCTTCCAACCCAAACAATGGTTCCTGTAGAAGATACACTCTAAGAATGGCTTGAATCCTCTATTCAAATTTATTCCCTCAAACTCATAAGAGAAATACTATTTTCTCTCAAGTTCACTCACTGTCGTCTCTGAATTACCGAAGAAAAACACTTCAGCCGCATGAGAGAGCTTTCAGCTGCACAAAGAAACGTCTCCTCTCTCAGTGAAAAATTCCCCACCCGCAAAAAATATATATGTATATATATATATATATATATATATATATATATATATATAAACACAACCTAATGGGCTTTAAGGCCCAAAACCTTCTCCTTTTATTTTTGTGCACTACAAGTTGAGGAAGGAGTTTTGTCTCCACACAATCCCATCATCCCCAACATCACCAACTCCGTGTAGTACACCAATGACACTTTAACTGCATCATTTTCGTTCTCAAAAACCACCTTACTGCTCATATACTCCTTCTCGAAAGTTGAAGGTTCTCGAAAGTTGAAGGTTCATGTCATCAGAGCATGATCCAAAATATTTGGCTCGCAAGTCTACGGTGGATGCACCCCTCTCCACTTCACTGTTAGAGTCCCCCACAGCTCGGTCATCAACATAAATTCATCATTCGAAAACGACACAATCGTGCCCCTCAGATTGAAGCTCATGACTTCAGGTCTGACATCCTCCACCTCCCTCAGCAAAATGTGATGGACCAACTGGTTGTTGAAGACCAGATCAACATCAACTAAGTGGCCGAAGATAGTAGTAGTTTTGAACAACTCCAATTGAGTCAAGGTCAGTTTGCTCTTCACAATTTCGTTCGCACTACGAATGTGCGAGAGGCACGTACCTTGGTGGGGGAACCGATCAACCAGGTTAATCTTCCATGTGGGTGCCATTATCCTGCGTAGTTCAACATGATCATAAGACCATGGCTACAAATTACAAAACACAATTAGTATGACTGTTACATCCAACAGTTCAAAACATTTTTCCTTGTTACACTTCTCACTACTCTTATGCCTAACCATTCTTGCTAACTATTAAACCTAACTATTATCATATAGCTGACATAAGTCACTAGCCCCTATACCTAGCGAGTTAGTCTCCCTGTGTTAAATTCGGCGTCCTCTCCATCATTCATTGGTGTCAACATTATCCCATCCTATTTTTCACTCTGTTCCCAAACCATTGAACCCTAGTCGCTAGCGAGTCAGTATCATCCCCTCTGTTTCTCCTTAATTTCTATTAGTTCTCTCCCCACTTCATTAGACATCTCGATTGTCCTAGATGTATCAACAATCACCCCCACCAAATCGGAGACAACAAACCATACGTAACCCTAAATCGACATTTCGATCAAATACACCCATCTAAAATATGGAATTACACTACATGCAGATACACATTTCTTCGAAACAGAGTAATGAACGGAGAAAGTCAATGAATGCTTACCAAGAACTGATGAACCGTCAATTTCGTTGTCCTTCACAAATCATCTGGATTTTCTTCGCAAATTGAAAGTCAGTAAAGTAAGATTCCTTTGCACTGAGTGATGAATGGAAATACAAACGGTTGTGTTGTTTTGTTCTATTGAATAGTGTGTCGCTTCACATTCAATTGATTCCCAACGATAGAAAAACTACTATAGAGACTGACATATAACATTGTTCCAACCAGTTGGGTTGGGATGTTCAGCCCGACAGGTTGCATTACAACCAGTCCGTGAGGTTGGAAGATAGTGACCACGACGAACCGGTAACCGAGGGAGCGAGTCAGGCTAGCAGTTGGAGAGCTGGGTTGGGGAAAGAGGGACCATTATAGTAATTTTGCCACTTGATGACATCATCAAAAGGTCAGATATCTTTCATTTCTAATATATGGGTTATATGACATTAGCGTTTTATCCGTACTTTTCCTTTTTTTTTTTCTTTTTTTCAACGCCATTTTCAACGCCAATCACAGGATTTCGCTTCAACTTTCTTCGTCTACAAGGTGCCTCTCTCTCTCTCTCTCTAAACTATTGGACATTTGAAATCGTAGTCTCGAGGTTAATCATTTAATCCTCACTCTCTTTGATGAATTTCGAAATACAGATTATGATTGTGTCATGAAGCAGAACAGAGAACAATACATGAACAGCGCGGAGTTCAGTTCAAATTGAACAGCTCCGCTCTGAAACTTTCTTGCTTTCTAATTTAGTTCTTCTGGGTAATGATTAGGGAGATGATGATTCTAAACTAGCAAATTGGTTTCGTTAATTATGTAGCTGCGTCTCTTCATTCGGCTACAAGGGTTGAGATTTGGCTTTGATTCTCTTTTCAACGTTTTGGGGCTTTTCTGTTGTCTAAAATTGCAGAACAATTGTACGTCTAGCTGCTTGAGCTTGCTGCTTCATTGCTTCATTCTCTTGTTGATGTTGTTCATGTGCAGTTTTTGAGCAATTCAGTCTTGTTTCTTATTGCAACGCCAATTTTTGCAGCTCTTTTCGGCTATGGATCTCAACGATCTCAATAAGGTTTGGGAAATTAAACCTTTGAAGAAGATTGGGGAAGAGGATGCTCGGAAAGTGCTTGAAAAAATAGCTAAACAGGTGCAACCTATCATGCGCAAACGCAGATGGAAAGTGGAAACTCTTTCTGAGTTCTAGTACTGACTTTTTTCGATTCTCTTTTAATTTAGAAGTATTTGGGTTTTTAGTTCTTCGTGTCATAATCTTGAATTTGAATTGCAGTCCTGACAATCCAGGTCTCATGGGGGTGAACATTGGAGGAGGTCAGGAAATCAAACTCAGAATTCGGAGGCCGAATAATGAGTGGGATTTCTTCCCTTATGAGCAGATTCTTGACACAATGCTCCATGAGCTTTGCCACATTGTACATGGCCCTCACAATGCTGATTTCTATAGCCTCTTAGATGAACTCAGAAAGGTGATTCTTTCTTTATTTACAGTTTGTCAAATGCCATTGTGTTTTTGGTTGGGTGGTTGCCCAAATGGTGTAAATTACTTCAACGACCTTCTGCACTTGGAAGAACTATTTAACTAGGCATTTAAGTTCAAAATCACAAGACTTTTCTCCTCTGAACTACTTAAAGGTGGGTTTACGAGGCTTTTGGAGGTTTTTTGAAGAATGCTTTTTAGCCCAGTAACAGCACTTTTCAAAAGTTTCTTCTATAAGCACTTCAGGGGAAAACTTCCAGTAGTGCTCATGGAAGAAAGGTTTTACTCAAAATCACTTTTTTCAAAAGAACAATTTTAAAAAGGAACCATGAATCATAATCATCTGTATCAAGCTGCAGTTGGATTGTGTGTGTCATTTAATAAAATTGTTCACTTTGTAACATGAACCATGGCTGTGTGTGCATATTGCAAAGCTCTGTACTTCAACAATGCTAAACTATGACAAGCTTCAAGGAATTCAATCACATGCATCATATGGAACATTATTTGGTCATTTGGATTGCTCATTCAATTTGGTCTGCCTTTTTGCCTCTGTGATATATGCCAATCACCTTTCATTCCTCCTTTTGCTGTAGGAATGCGAAGAACTTATGTGTAAAGGTATCACGGGCACTGGACAAGGATTCGATCTTCGTGGGAGACGTTTAGGTGGGATCTCTCAGCAACCACCATTATCATCCCTCAGGCAAACTACCTTATCTGCTGCTGAAAACCGAGCTCGAGGTGGTCCATCGGGACCTAAGCGCCTTGGTGGTGACAGCAACATGAAGGCAGTGCTTAGTCCTATACAAGCAGCCGCCATGGCAGCAGAAAGAAGACTACAAGATGATTTATGGTGTGGTTCCAAGTCATTGGAGAACAATTCAGATAGAGCTAAAAACATGAGTTCCTCCATTGGACCCTCCAGAACTTCAAATGTTTTCACACCTTCTGTTATATCCCAAATTCCATCTGCTGCTTCCTTTCCTCCTCAGGGAGCTATGGATGATCCGGAAAATTGGCAGTGCAGTGCATGTACTCTTCTAAATCGGGTAAGTTTCCTTTGCTGTTTGTTTTCATTTATACGTCGGTCAAATTTGGTTCTTGAACTTTTAAAGTTGGGTCTATTTAGTTTTTGTTTTAAAATTAGTTTCTAGCAAATTCTTGAAAATTTTGAACTTATGTTAATAACTGAAATCATTTTAAAGAATTATACAGCAGGTTCATGTGTTTGAATTTTGATACTGCAAGTATTTGTCGAAGTTAACAACATAGTTTTATTATTAGACACAAAATTGAATGTTTAACGGTCTATTATAATCTTTTTAAATTACAGGAACTAAATAGACACAATCCTCAAAGTTAGTGATCTACTTGAAACTTTTTAAAATATAAATGCTAAATAGGTACAACCTTGAAAGTTTAGAGACCAAACCTACCATTTAAACTTTCAAGGTTTATCCGACGGGGTTGTCTAGATTACATCCTCACTCCATTTTTTTTTAAAAAAAACTTGGATTGCAGCCATTGGCTCTGATTTGTGAAGCTTGTGGGAACCAGAAGAAGAAAAGCAACACAAAGACTTGGGCTTGCAAGTTCTGTACGCTCAACAACAGTACTGATGCAGAGAGATGCTTAGCTTGTGGGGAATGGAGATATTCATATGGCCCTCCCATCTCCACACCTGGTCCTTATATTGGCACTTGAGAATAAATCATCTGTCCATTCATACATTGATTTCTACAGGTACATAATTTAATATTTTATCATACACAAAGTGATGTATTTACTTAAGAAATGGAGCCTACATATTTTCTATACTTGGGCTGCCCTTATTTTTAAGGGTATGGTCTTTTGAATGTTGGAGACTGAAGGTTGCCATTTCTGTACATAGCTACTACTCTTGCATATACAGCTTTTCTGTATGTAATAAGCTTTTCGTTAAATTATTAAAAGTACCCCCGAACTATGGGATTGGTTTCAATTATACCTTCAAACTTTGAAAAGTTTCATTTTAATCCC

mRNA sequence

ATGTGGGGCAACGCATCACACCTCACTCGTTTTGCAACTTTGCCTTCTATAGTCGATAGGGTCTCTTATGCCCATACCTATAACAAAAATACACATGCCTTAGTTTGCTTGGAAATCATGGGGACTGCCACAGATATGCATACATCTGTAGACACATACCCGATACACAAGCATGCATTAGTTTACCTGGAACGCATGGGGAAAGTCGTTAAGACTGTATTTCACTATGTGGTCGCTCTTCTCCTTCACCTTTTTAAACGTGTCCACCATGTCCTTGAGGGCACTTTGCAGCCCCTTGATTGTCCTCTCCCAAATTCAAGTACCCTATTGGGTTGGGATGTTCAGCCCGACAGGTTGCATTACAACCAGTCCGTGAGGTTGGAAGATAGTGACCACGACGAACCGCTCTTTTCGGCTATGGATCTCAACGATCTCAATAAGGTTTGGGAAATTAAACCTTTGAAGAAGATTGGGGAAGAGGATGCTCGGAAAGTGCTTGAAAAAATAGCTAAACAGGTGCAACCTATCATGCGCAAACGCAGATGGAAAGTGGAAACTCTTTCTGAGTTCTATCCTGACAATCCAGGTCTCATGGGGGTGAACATTGGAGGAGGTCAGGAAATCAAACTCAGAATTCGGAGGCCGAATAATGAGTGGGATTTCTTCCCTTATGAGCAGATTCTTGACACAATGCTCCATGAGCTTTGCCACATTGTACATGGCCCTCACAATGCTGATTTCTATAGCCTCTTAGATGAACTCAGAAAGGAATGCGAAGAACTTATGTGTAAAGGTATCACGGGCACTGGACAAGGATTCGATCTTCGTGGGAGACGTTTAGGTGGGATCTCTCAGCAACCACCATTATCATCCCTCAGGCAAACTACCTTATCTGCTGCTGAAAACCGAGCTCGAGGTGGTCCATCGGGACCTAAGCGCCTTGGTGGTGACAGCAACATGAAGGCAGTGCTTAGTCCTATACAAGCAGCCGCCATGGCAGCAGAAAGAAGACTACAAGATGATTTATGGTGTGGTTCCAAGTCATTGGAGAACAATTCAGATAGAGCTAAAAACATGAGTTCCTCCATTGGACCCTCCAGAACTTCAAATGTTTTCACACCTTCTGTTATATCCCAAATTCCATCTGCTGCTTCCTTTCCTCCTCAGGGAGCTATGGATGATCCGGAAAATTGGCAGTGCAGTGCATGTACTCTTCTAAATCGGCCATTGGCTCTGATTTGTGAAGCTTGTGGGAACCAGAAGAAGAAAAGCAACACAAAGACTTGGGCTTGCAAGTTCTGTACGCTCAACAACAGTACTGATGCAGAGAGATGCTTAGCTTGTGGGGAATGGAGATATTCATATGGCCCTCCCATCTCCACACCTGGTCCTTATATTGGCACTTGA

Coding sequence (CDS)

ATGTGGGGCAACGCATCACACCTCACTCGTTTTGCAACTTTGCCTTCTATAGTCGATAGGGTCTCTTATGCCCATACCTATAACAAAAATACACATGCCTTAGTTTGCTTGGAAATCATGGGGACTGCCACAGATATGCATACATCTGTAGACACATACCCGATACACAAGCATGCATTAGTTTACCTGGAACGCATGGGGAAAGTCGTTAAGACTGTATTTCACTATGTGGTCGCTCTTCTCCTTCACCTTTTTAAACGTGTCCACCATGTCCTTGAGGGCACTTTGCAGCCCCTTGATTGTCCTCTCCCAAATTCAAGTACCCTATTGGGTTGGGATGTTCAGCCCGACAGGTTGCATTACAACCAGTCCGTGAGGTTGGAAGATAGTGACCACGACGAACCGCTCTTTTCGGCTATGGATCTCAACGATCTCAATAAGGTTTGGGAAATTAAACCTTTGAAGAAGATTGGGGAAGAGGATGCTCGGAAAGTGCTTGAAAAAATAGCTAAACAGGTGCAACCTATCATGCGCAAACGCAGATGGAAAGTGGAAACTCTTTCTGAGTTCTATCCTGACAATCCAGGTCTCATGGGGGTGAACATTGGAGGAGGTCAGGAAATCAAACTCAGAATTCGGAGGCCGAATAATGAGTGGGATTTCTTCCCTTATGAGCAGATTCTTGACACAATGCTCCATGAGCTTTGCCACATTGTACATGGCCCTCACAATGCTGATTTCTATAGCCTCTTAGATGAACTCAGAAAGGAATGCGAAGAACTTATGTGTAAAGGTATCACGGGCACTGGACAAGGATTCGATCTTCGTGGGAGACGTTTAGGTGGGATCTCTCAGCAACCACCATTATCATCCCTCAGGCAAACTACCTTATCTGCTGCTGAAAACCGAGCTCGAGGTGGTCCATCGGGACCTAAGCGCCTTGGTGGTGACAGCAACATGAAGGCAGTGCTTAGTCCTATACAAGCAGCCGCCATGGCAGCAGAAAGAAGACTACAAGATGATTTATGGTGTGGTTCCAAGTCATTGGAGAACAATTCAGATAGAGCTAAAAACATGAGTTCCTCCATTGGACCCTCCAGAACTTCAAATGTTTTCACACCTTCTGTTATATCCCAAATTCCATCTGCTGCTTCCTTTCCTCCTCAGGGAGCTATGGATGATCCGGAAAATTGGCAGTGCAGTGCATGTACTCTTCTAAATCGGCCATTGGCTCTGATTTGTGAAGCTTGTGGGAACCAGAAGAAGAAAAGCAACACAAAGACTTGGGCTTGCAAGTTCTGTACGCTCAACAACAGTACTGATGCAGAGAGATGCTTAGCTTGTGGGGAATGGAGATATTCATATGGCCCTCCCATCTCCACACCTGGTCCTTATATTGGCACTTGA

Protein sequence

MWGNASHLTRFATLPSIVDRVSYAHTYNKNTHALVCLEIMGTATDMHTSVDTYPIHKHALVYLERMGKVVKTVFHYVVALLLHLFKRVHHVLEGTLQPLDCPLPNSSTLLGWDVQPDRLHYNQSVRLEDSDHDEPLFSAMDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMGVNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECEELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSNMKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSRTSNVFTPSVISQIPSAASFPPQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLNNSTDAERCLACGEWRYSYGPPISTPGPYIGT

Homology

[Close]

BLAST of Spg020028 vs. NCBI nr
Match: XP_022967821.1 (DNA-dependent metalloprotease WSS1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 630.2 bits (1624), Expect = 1.4e-176
Identity = 306/342 (89.47%), Postives = 322/342 (94.15%), Query Frame = 0


Query: 128 EDSDHDEPLFSAMDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETL 187
           + S  D+ L S MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKRRWKVETL
Sbjct: 12  QSSFFDDKLLSLMDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRRWKVETL 71

Query: 188 SEFYPDNPGLMGVNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADF 247
           SEFYPDNPGLMGVNIGGGQEIKLRIRRPN+EWDFFPYEQILDTMLHELCHIVHGPHNADF
Sbjct: 72  SEFYPDNPGLMGVNIGGGQEIKLRIRRPNSEWDFFPYEQILDTMLHELCHIVHGPHNADF 131

Query: 248 YSLLDELRKECEELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGG 307
           Y+LLDELRKECEELMCKGITGTGQGFD+RGRRLGGIS Q PLSSLRQTTL+AAENRARG 
Sbjct: 132 YNLLDELRKECEELMCKGITGTGQGFDVRGRRLGGISHQLPLSSLRQTTLTAAENRARGA 191

Query: 308 PSGPKRLGGDSNMKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSR 367
           PSGPKRLGGDS MKAVLSP+QAAAMAAERRL+DDLWCGSKSLENNSDRAKNMSSS GPSR
Sbjct: 192 PSGPKRLGGDSKMKAVLSPVQAAAMAAERRLKDDLWCGSKSLENNSDRAKNMSSSTGPSR 251

Query: 368 TSNVFTPSVISQIPSAASFP-PQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNT 427
           TSNVFTP + SQIPSAASFP PQ AM D ENWQCSACTLLN+PLALICEACGN+K K NT
Sbjct: 252 TSNVFTP-LTSQIPSAASFPTPQEAMSDLENWQCSACTLLNQPLALICEACGNRKNKMNT 311

Query: 428 KTWACKFCTLNNSTDAERCLACGEWRYSYGPPISTPGPYIGT 469
           KTWACKFCTL+NSTDA+RCLACGEWRYSYGPP++T GPYIGT
Sbjct: 312 KTWACKFCTLSNSTDADRCLACGEWRYSYGPPLATQGPYIGT 352

[Close]

BLAST of Spg020028 vs. NCBI nr
Match: XP_022967822.1 (DNA-dependent metalloprotease WSS1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 624.4 bits (1609), Expect = 8.0e-175
Identity = 302/330 (91.52%), Postives = 316/330 (95.76%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG
Sbjct: 1   MDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNIGGGQEIKLRIRRPN+EWDFFPYEQILDTMLHELCHIVHGPHNADFY+LLDELRKECE
Sbjct: 61  VNIGGGQEIKLRIRRPNSEWDFFPYEQILDTMLHELCHIVHGPHNADFYNLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELMCKGITGTGQGFD+RGRRLGGIS Q PLSSLRQTTL+AAENRARG PSGPKRLGGDS 
Sbjct: 121 ELMCKGITGTGQGFDVRGRRLGGISHQLPLSSLRQTTLTAAENRARGAPSGPKRLGGDSK 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSRTSNVFTPSVISQ 379
           MKAVLSP+QAAAMAAERRL+DDLWCGSKSLENNSDRAKNMSSS GPSRTSNVFTP + SQ
Sbjct: 181 MKAVLSPVQAAAMAAERRLKDDLWCGSKSLENNSDRAKNMSSSTGPSRTSNVFTP-LTSQ 240

Query: 380 IPSAASFP-PQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLNN 439
           IPSAASFP PQ AM D ENWQCSACTLLN+PLALICEACGN+K K NTKTWACKFCTL+N
Sbjct: 241 IPSAASFPTPQEAMSDLENWQCSACTLLNQPLALICEACGNRKNKMNTKTWACKFCTLSN 300

Query: 440 STDAERCLACGEWRYSYGPPISTPGPYIGT 469
           STDA+RCLACGEWRYSYGPP++T GPYIGT
Sbjct: 301 STDADRCLACGEWRYSYGPPLATQGPYIGT 329

[Close]

BLAST of Spg020028 vs. NCBI nr
Match: XP_022928615.1 (DNA-dependent metalloprotease WSS1-like isoform X1 [Cucurbita moschata] >XP_022928616.1 DNA-dependent metalloprotease WSS1-like isoform X1 [Cucurbita moschata] >XP_022928617.1 DNA-dependent metalloprotease WSS1-like isoform X1 [Cucurbita moschata] >KAG6603250.1 hypothetical protein SDJN03_03859, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033552.1 WSS1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 621.7 bits (1602), Expect = 5.2e-174
Identity = 301/330 (91.21%), Postives = 314/330 (95.15%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG
Sbjct: 1   MDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNIGGGQEIKLRIRRPN+EWDFFPYEQILDTMLHELCHIVHGPHNADFY+LLDELRKECE
Sbjct: 61  VNIGGGQEIKLRIRRPNSEWDFFPYEQILDTMLHELCHIVHGPHNADFYNLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELMCKGITGTGQGFD+RGRRLGGIS Q PLSSLRQTTL+AAENRARG PSGPKRLGGDS 
Sbjct: 121 ELMCKGITGTGQGFDVRGRRLGGISHQLPLSSLRQTTLTAAENRARGAPSGPKRLGGDSK 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSRTSNVFTPSVISQ 379
           MKAVLSP+QAAAMAAERRL+DDLWCGSKSLENNSDRAKNMSSS GPSRTSNVFTP V SQ
Sbjct: 181 MKAVLSPVQAAAMAAERRLKDDLWCGSKSLENNSDRAKNMSSSTGPSRTSNVFTP-VTSQ 240

Query: 380 IPSAASFP-PQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLNN 439
           IPSAASFP PQ A  D ENWQCSACTLLN+PLALICEACGN+K K NTKTW CKFCTL+N
Sbjct: 241 IPSAASFPTPQEARSDLENWQCSACTLLNQPLALICEACGNRKNKMNTKTWGCKFCTLSN 300

Query: 440 STDAERCLACGEWRYSYGPPISTPGPYIGT 469
           STDA+RCLACGEWRYSYGPP++T GPYIGT
Sbjct: 301 STDADRCLACGEWRYSYGPPLATQGPYIGT 329

[Close]

BLAST of Spg020028 vs. NCBI nr
Match: XP_023543567.1 (DNA-dependent metalloprotease WSS1-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023543568.1 DNA-dependent metalloprotease WSS1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.7 bits (1589), Expect = 1.7e-172
Identity = 299/330 (90.61%), Postives = 312/330 (94.55%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG
Sbjct: 1   MDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNI GGQEIKLRIRRPN+EWDFFPYEQILDTMLHELCHIVHGPHNADFY+LLDELRKECE
Sbjct: 61  VNIAGGQEIKLRIRRPNSEWDFFPYEQILDTMLHELCHIVHGPHNADFYNLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELMCKGITGTGQGFD+RGRRLGGIS Q PLSSLRQTTL+AAENRARG PSGPKRLGGDS 
Sbjct: 121 ELMCKGITGTGQGFDVRGRRLGGISHQLPLSSLRQTTLTAAENRARGAPSGPKRLGGDSK 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSRTSNVFTPSVISQ 379
           MKAVLSP+QAAAMAAERRL+DDLWCGSKSLENNSDRAKNMSSS GPSRTSNVFTP V SQ
Sbjct: 181 MKAVLSPVQAAAMAAERRLKDDLWCGSKSLENNSDRAKNMSSSTGPSRTSNVFTP-VTSQ 240

Query: 380 IPSAASF-PPQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLNN 439
           IPSAASF  PQ AM D ENWQCSACTLLN+PL L CEACGN+K K NTKTWACKFCTL+N
Sbjct: 241 IPSAASFATPQEAMSDLENWQCSACTLLNQPLTLTCEACGNRKNKMNTKTWACKFCTLSN 300

Query: 440 STDAERCLACGEWRYSYGPPISTPGPYIGT 469
           STDA+RCLACGEWRYSYGPP++T GPYIGT
Sbjct: 301 STDADRCLACGEWRYSYGPPLATQGPYIGT 329

[Close]

BLAST of Spg020028 vs. NCBI nr
Match: XP_022151616.1 (DNA-dependent metalloprotease WSS1-like isoform X1 [Momordica charantia] >XP_022151618.1 DNA-dependent metalloprotease WSS1-like isoform X1 [Momordica charantia])

HSP 1 Score: 608.6 bits (1568), Expect = 4.5e-170
Identity = 295/331 (89.12%), Postives = 309/331 (93.35%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKR+WKVETLSEFYP NPGLMG
Sbjct: 1   MDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRKWKVETLSEFYPANPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE
Sbjct: 61  VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELMCKGIT TGQGFDL GRRLGGISQQPPLSSLRQTTL+AAENRARGGPSGPKRLGGDSN
Sbjct: 121 ELMCKGITRTGQGFDLHGRRLGGISQQPPLSSLRQTTLAAAENRARGGPSGPKRLGGDSN 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSR-TSNVFTPSVIS 379
           +KAVLSPIQAAAMAAERRL+DDLWCGSKSLENNS RAKN+ SS G S+ TSN   P VIS
Sbjct: 181 IKAVLSPIQAAAMAAERRLKDDLWCGSKSLENNSGRAKNLGSSTGSSQTTSNASAPPVIS 240

Query: 380 QIPSA-ASFPPQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLN 439
           QIPSA +SFP Q AMDD ENWQCSACTLLNRPLALICEACGN+K ++NTKTW CKFCT N
Sbjct: 241 QIPSATSSFPHQEAMDDMENWQCSACTLLNRPLALICEACGNRKNRNNTKTWGCKFCTFN 300

Query: 440 NSTDAERCLACGEWRYSYGPPISTPGPYIGT 469
           NS D +RCLACGEWRYSYGPPIST GP++GT
Sbjct: 301 NSADTDRCLACGEWRYSYGPPISTRGPFVGT 331

[Close]

BLAST of Spg020028 vs. ExPASy Swiss-Prot
Match: P38838 (DNA-dependent metalloprotease WSS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=WSS1 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 5.2e-28
Identity = 81/214 (37.85%), Postives = 121/214 (56.54%), Query Frame = 0


Query: 156 KIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMGVNIGGGQEIKLRIRRP 215
           K  +EDA  ++++IA +V  +M++  +KV  L EFYP +  L+G+N+  G +I LR+R  
Sbjct: 38  KPNKEDALNLIKEIAHKVSYLMKENHFKVTNLVEFYPRDQRLLGMNVNHGSKIMLRLRCS 97

Query: 216 NNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECEELMCKGITGTGQGFDL 275
            +E+ F P E I+ TMLHEL H + GPH+  FY+ LDEL      +  +G+  T  G   
Sbjct: 98  TDEFQFLPMECIMGTMLHELTHNLFGPHDKKFYNKLDELIGRQWVIEQRGLYDTFLG--- 157

Query: 276 RGRRLGG----ISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSNMKAVLSPIQAAA 335
            G+RLGG     S + P++ +  T       R +G   G     G S++    SP + AA
Sbjct: 158 NGQRLGGRANLRSNRYPMTGI-STNTGIVRKRGKGVKLGSLHPEGISSIDRGNSPRELAA 217

Query: 336 MAAERRLQDDLWCGSKSLENNSDR--AKNMSSSI 364
            AAERR +DD WCG    +NN D+  + N+SSS+
Sbjct: 218 FAAERRYRDDRWCG--ETKNNKDQIISDNISSSL 245

[Close]

BLAST of Spg020028 vs. ExPASy Swiss-Prot
Match: Q9P7B5 (DNA-dependent metalloprotease WSS1 homolog OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC521.02 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 5.2e-12
Identity = 48/147 (32.65%), Postives = 70/147 (47.62%), Query Frame = 0


Query: 125 VRLEDSDHDEPLFSAMDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKV 184
           +R+ D DH              K+  I  +K    + +   L++IA    PIM++  + V
Sbjct: 23  LRINDDDHPN-----------EKIGFISAIKGDFHDLSSDYLKRIAAMAFPIMKEHGFGV 82

Query: 185 ETLSEFYPDNPGLMGVNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHN 244
            +L E    N    G N   G+ I+L +R  +N W   P+E ++D  LHELCHI  GPH+
Sbjct: 83  TSLDE-VAYNAKFWGRNWNKGECIELVLRDASNRW--LPFEFVMDVFLHELCHIWQGPHD 142

Query: 245 ADFYSLLDELRKECEELMCKGITGTGQ 272
             F+S L  LR     L  KG  G G+
Sbjct: 143 RRFFSHLSTLRAALIALYAKGYKGPGK 155

[Close]

BLAST of Spg020028 vs. ExPASy Swiss-Prot
Match: O94580 (DNA-dependent metalloprotease WSS1 homolog 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=wss2 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.6e-08
Identity = 44/156 (28.21%), Postives = 71/156 (45.51%), Query Frame = 0


Query: 159 EEDARKVLEKIAKQ--VQPIMRKRRWKVETLSEFYP-----DNPGLMGVNIGGGQEIKLR 218
           ++ A + LE++     ++ IM   RW V  LSE  P      +   +G+N   G  I+LR
Sbjct: 122 KDRALRYLERLRDDTGIKKIMDSHRWTVPLLSEMDPAEHTRHDSKTLGLNHNQGAHIELR 181

Query: 219 IRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECEELMCKGITGTGQ 278
           +R    +  F  Y+ +  T++HEL H VHG H++ F+ L  +L KE +     G  G+  
Sbjct: 182 LRTDRYD-GFRDYKTVKSTLIHELTHNVHGEHDSSFWELFRQLTKEADAADLLGKPGSYV 241

Query: 279 GFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGG 308
                         +   +  R   L+AAE R + G
Sbjct: 242 SDRASYTPQQDNDDEDQKNHRRDLLLAAAERRKQSG 276

[Close]

BLAST of Spg020028 vs. ExPASy TrEMBL
Match: A0A6J1HT53 (DNA-dependent metalloprotease WSS1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467224 PE=4 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 7.0e-177
Identity = 306/342 (89.47%), Postives = 322/342 (94.15%), Query Frame = 0


Query: 128 EDSDHDEPLFSAMDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETL 187
           + S  D+ L S MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKRRWKVETL
Sbjct: 12  QSSFFDDKLLSLMDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRRWKVETL 71

Query: 188 SEFYPDNPGLMGVNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADF 247
           SEFYPDNPGLMGVNIGGGQEIKLRIRRPN+EWDFFPYEQILDTMLHELCHIVHGPHNADF
Sbjct: 72  SEFYPDNPGLMGVNIGGGQEIKLRIRRPNSEWDFFPYEQILDTMLHELCHIVHGPHNADF 131

Query: 248 YSLLDELRKECEELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGG 307
           Y+LLDELRKECEELMCKGITGTGQGFD+RGRRLGGIS Q PLSSLRQTTL+AAENRARG 
Sbjct: 132 YNLLDELRKECEELMCKGITGTGQGFDVRGRRLGGISHQLPLSSLRQTTLTAAENRARGA 191

Query: 308 PSGPKRLGGDSNMKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSR 367
           PSGPKRLGGDS MKAVLSP+QAAAMAAERRL+DDLWCGSKSLENNSDRAKNMSSS GPSR
Sbjct: 192 PSGPKRLGGDSKMKAVLSPVQAAAMAAERRLKDDLWCGSKSLENNSDRAKNMSSSTGPSR 251

Query: 368 TSNVFTPSVISQIPSAASFP-PQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNT 427
           TSNVFTP + SQIPSAASFP PQ AM D ENWQCSACTLLN+PLALICEACGN+K K NT
Sbjct: 252 TSNVFTP-LTSQIPSAASFPTPQEAMSDLENWQCSACTLLNQPLALICEACGNRKNKMNT 311

Query: 428 KTWACKFCTLNNSTDAERCLACGEWRYSYGPPISTPGPYIGT 469
           KTWACKFCTL+NSTDA+RCLACGEWRYSYGPP++T GPYIGT
Sbjct: 312 KTWACKFCTLSNSTDADRCLACGEWRYSYGPPLATQGPYIGT 352

[Close]

BLAST of Spg020028 vs. ExPASy TrEMBL
Match: A0A6J1HRV8 (DNA-dependent metalloprotease WSS1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467224 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.8e-175
Identity = 302/330 (91.52%), Postives = 316/330 (95.76%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG
Sbjct: 1   MDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNIGGGQEIKLRIRRPN+EWDFFPYEQILDTMLHELCHIVHGPHNADFY+LLDELRKECE
Sbjct: 61  VNIGGGQEIKLRIRRPNSEWDFFPYEQILDTMLHELCHIVHGPHNADFYNLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELMCKGITGTGQGFD+RGRRLGGIS Q PLSSLRQTTL+AAENRARG PSGPKRLGGDS 
Sbjct: 121 ELMCKGITGTGQGFDVRGRRLGGISHQLPLSSLRQTTLTAAENRARGAPSGPKRLGGDSK 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSRTSNVFTPSVISQ 379
           MKAVLSP+QAAAMAAERRL+DDLWCGSKSLENNSDRAKNMSSS GPSRTSNVFTP + SQ
Sbjct: 181 MKAVLSPVQAAAMAAERRLKDDLWCGSKSLENNSDRAKNMSSSTGPSRTSNVFTP-LTSQ 240

Query: 380 IPSAASFP-PQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLNN 439
           IPSAASFP PQ AM D ENWQCSACTLLN+PLALICEACGN+K K NTKTWACKFCTL+N
Sbjct: 241 IPSAASFPTPQEAMSDLENWQCSACTLLNQPLALICEACGNRKNKMNTKTWACKFCTLSN 300

Query: 440 STDAERCLACGEWRYSYGPPISTPGPYIGT 469
           STDA+RCLACGEWRYSYGPP++T GPYIGT
Sbjct: 301 STDADRCLACGEWRYSYGPPLATQGPYIGT 329

[Close]

BLAST of Spg020028 vs. ExPASy TrEMBL
Match: A0A6J1EKF1 (DNA-dependent metalloprotease WSS1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435471 PE=4 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 2.5e-174
Identity = 301/330 (91.21%), Postives = 314/330 (95.15%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG
Sbjct: 1   MDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNIGGGQEIKLRIRRPN+EWDFFPYEQILDTMLHELCHIVHGPHNADFY+LLDELRKECE
Sbjct: 61  VNIGGGQEIKLRIRRPNSEWDFFPYEQILDTMLHELCHIVHGPHNADFYNLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELMCKGITGTGQGFD+RGRRLGGIS Q PLSSLRQTTL+AAENRARG PSGPKRLGGDS 
Sbjct: 121 ELMCKGITGTGQGFDVRGRRLGGISHQLPLSSLRQTTLTAAENRARGAPSGPKRLGGDSK 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSRTSNVFTPSVISQ 379
           MKAVLSP+QAAAMAAERRL+DDLWCGSKSLENNSDRAKNMSSS GPSRTSNVFTP V SQ
Sbjct: 181 MKAVLSPVQAAAMAAERRLKDDLWCGSKSLENNSDRAKNMSSSTGPSRTSNVFTP-VTSQ 240

Query: 380 IPSAASFP-PQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLNN 439
           IPSAASFP PQ A  D ENWQCSACTLLN+PLALICEACGN+K K NTKTW CKFCTL+N
Sbjct: 241 IPSAASFPTPQEARSDLENWQCSACTLLNQPLALICEACGNRKNKMNTKTWGCKFCTLSN 300

Query: 440 STDAERCLACGEWRYSYGPPISTPGPYIGT 469
           STDA+RCLACGEWRYSYGPP++T GPYIGT
Sbjct: 301 STDADRCLACGEWRYSYGPPLATQGPYIGT 329

[Close]

BLAST of Spg020028 vs. ExPASy TrEMBL
Match: A0A6J1DF71 (DNA-dependent metalloprotease WSS1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019534 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 2.2e-170
Identity = 295/331 (89.12%), Postives = 309/331 (93.35%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVWEIKPLKKIGE+DARKVLEKIAKQVQPIMRKR+WKVETLSEFYP NPGLMG
Sbjct: 1   MDLNDLNKVWEIKPLKKIGEDDARKVLEKIAKQVQPIMRKRKWKVETLSEFYPANPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE
Sbjct: 61  VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELMCKGIT TGQGFDL GRRLGGISQQPPLSSLRQTTL+AAENRARGGPSGPKRLGGDSN
Sbjct: 121 ELMCKGITRTGQGFDLHGRRLGGISQQPPLSSLRQTTLAAAENRARGGPSGPKRLGGDSN 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSR-TSNVFTPSVIS 379
           +KAVLSPIQAAAMAAERRL+DDLWCGSKSLENNS RAKN+ SS G S+ TSN   P VIS
Sbjct: 181 IKAVLSPIQAAAMAAERRLKDDLWCGSKSLENNSGRAKNLGSSTGSSQTTSNASAPPVIS 240

Query: 380 QIPSA-ASFPPQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLN 439
           QIPSA +SFP Q AMDD ENWQCSACTLLNRPLALICEACGN+K ++NTKTW CKFCT N
Sbjct: 241 QIPSATSSFPHQEAMDDMENWQCSACTLLNRPLALICEACGNRKNRNNTKTWGCKFCTFN 300

Query: 440 NSTDAERCLACGEWRYSYGPPISTPGPYIGT 469
           NS D +RCLACGEWRYSYGPPIST GP++GT
Sbjct: 301 NSADTDRCLACGEWRYSYGPPISTRGPFVGT 331

[Close]

BLAST of Spg020028 vs. ExPASy TrEMBL
Match: A0A1S4DV14 (LOW QUALITY PROTEIN: DNA-dependent metalloprotease WSS1-like OS=Cucumis melo OX=3656 GN=LOC103486153 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 2.8e-165
Identity = 290/329 (88.15%), Postives = 300/329 (91.19%), Query Frame = 0


Query: 140 MDLNDLNKVWEIKPLKKIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 199
           MDLNDLNKVW++ PLKKIGE+D+RKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG
Sbjct: 1   MDLNDLNKVWQVNPLKKIGEDDSRKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPGLMG 60

Query: 200 VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 259
           VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE
Sbjct: 61  VNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRKECE 120

Query: 260 ELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGGPSGPKRLGGDSN 319
           ELM KGITGTGQGFDLRGRRLGGIS QPPLSSLRQT L+AAENRARGGPSGPKRLGG SN
Sbjct: 121 ELMSKGITGTGQGFDLRGRRLGGISHQPPLSSLRQTALAAAENRARGGPSGPKRLGGXSN 180

Query: 320 MKAVLSPIQAAAMAAERRLQDDLWCGSKSLENNSDRAKNMSSSIGPSRTSNVFTPSVISQ 379
           MKAVLSPIQAAAMAAERRL+DDLWCGSKS ENNSDRAKNM SS G SR SN FTP VISQ
Sbjct: 181 MKAVLSPIQAAAMAAERRLKDDLWCGSKSWENNSDRAKNMGSSTGSSRASNAFTP-VISQ 240

Query: 380 IPSAASFPP-QGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNTKTWACKFCTLNN 439
           IPSA S P  Q A DD ENWQCS CTLLNR LALICEACGN K K NT+TWAC FCTL+N
Sbjct: 241 IPSATSIPTHQEATDDLENWQCSTCTLLNRSLALICEACGNHKNKMNTRTWACNFCTLSN 300

Query: 440 STDAERCLACGEWRYSYGPPISTPGPYIG 468
           S+DAE+CLACGEWRYSYGP IST  P IG
Sbjct: 301 SSDAEKCLACGEWRYSYGPSISTSPPDIG 328

[Close]

BLAST of Spg020028 vs. TAIR 10
Match: AT1G55915.1 (zinc ion binding )

HSP 1 Score: 362.8 bits (930), Expect = 4.0e-100
Identity = 201/403 (49.88%), Postives = 245/403 (60.79%), Query Frame = 0


Query: 138 SAMDLNDLNKVWEIKPLK-KIGEEDARKVLEKIAKQVQPIMRKRRWKVETLSEFYPDNPG 197
           S+ +L DLNKVWEIK LK K  E++ARK+LEK+A QVQPIM +R+W+V+ LSEF P NP 
Sbjct: 2   SSANLEDLNKVWEIKALKRKPREDEARKILEKVANQVQPIMTRRKWRVKLLSEFCPTNPR 61

Query: 198 LMGVNIGGGQEIKLRIRRPNNEWDFFPYEQILDTMLHELCHIVHGPHNADFYSLLDELRK 257
           L+GVN+  G ++KLR+RR N++ DF  Y +ILDTMLHELCH  HGPHNA FY L DELRK
Sbjct: 62  LLGVNVNRGVQVKLRLRRVNHDLDFLSYHEILDTMLHELCHNAHGPHNASFYKLWDELRK 121

Query: 258 ECEELMCKGITGTGQGFDLRGRRLGGISQQPPLSSLRQTTLSAAENRARGG---PSGPKR 317
           ECEELM KGITGTGQGFD+ G+RLGG+S+QP LS LR T  +AAE R R G   PSGP+R
Sbjct: 122 ECEELMSKGITGTGQGFDMPGKRLGGLSRQPSLSFLRATAATAAEKRVRAGTLLPSGPQR 181

Query: 318 LGGDSNMKAVLSPIQAAAMAAERRLQDDLWCGSKSL------ENNSDRAKNMSS------ 377
           LGGDS++ + LSPIQAAAMAAERRL DD+WCGS+S       EN+SD  K   S      
Sbjct: 182 LGGDSSIMSDLSPIQAAAMAAERRLLDDIWCGSQSTDALEDEENDSDTYKEPVSIRETCT 241

Query: 378 -----------------SIGPS-------------------------RTSNVFTPSVISQ 437
                            S  PS                         R+     PS    
Sbjct: 242 SVNGKSVKRCNSWSNAHSCPPSSSHQQGSDVIDLTEESFEIRCTKRNRSPGDQGPSCAKD 301

Query: 438 IPSA-----------ASFPPQGAMDDPENWQCSACTLLNRPLALICEACGNQKKKSNT-- 469
           +P+A            S+    + ++   W+C+ CTLLN  LA ICE C   K K     
Sbjct: 302 VPNAGVMKSSIPLPSTSYNANQSREESTMWECAECTLLNPSLAPICELCTAAKPKEREMK 361

[Close]

BLAST of Spg020028 vs. TAIR 10
Match: AT5G35690.1 (CONTAINS InterPro DOMAIN/s: WLM (InterPro:IPR013536), PUB domain (InterPro:IPR018997), PUG domain (InterPro:IPR006567); BEST Arabidopsis thaliana protein match is: zinc ion binding (TAIR:AT1G55915.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 70.5 bits (171), Expect = 4.1e-12
Identity = 49/151 (32.45%), Postives = 75/151 (49.67%), Query Frame = 0


Query: 176 IMRKRRWKVETLSEFYP------DNPGLMGVNIGGGQEIKLRIRRPNNEWDFFPYEQILD 235
           +M K RW+V  ++E  P          L+G N   G+EI LR+R  + +  F  Y+ I  
Sbjct: 180 VMNKHRWRVGIMTELAPVGYVGVSPRCLLGFNKNQGEEISLRLRTDDLK-GFRKYQSIKK 239

Query: 236 TMLHELCHIVHGPHNADFYSLLDELRKECEEL---MCKGITGTGQGF------------- 295
           T+LHEL H+V+  H+  FY+L  +L KE E L     +G T  G  F             
Sbjct: 240 TLLHELAHMVYTEHDEKFYALDSQLNKEAESLDWTKSRGHTLNGTKFINDDDEEDYFFDE 299

Query: 296 -DLRGRRLGGISQQPPLSSLRQTTLSAAENR 304
            +   +RLGG +Q   L + R+++++AA  R
Sbjct: 300 NETVSQRLGG-NQSDNLGNARESSVAAAYRR 328

The following BLAST results are available for this feature:

BLAST of Spg020028 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022967821.1	1.4e-176	89.47	DNA-dependent metalloprotease WSS1-like isoform X1 [Cucurbita maxima]	[more]
XP_022967822.1	8.0e-175	91.52	DNA-dependent metalloprotease WSS1-like isoform X2 [Cucurbita maxima]	[more]
XP_022928615.1	5.2e-174	91.21	DNA-dependent metalloprotease WSS1-like isoform X1 [Cucurbita moschata] >XP_0229...	[more]
XP_023543567.1	1.7e-172	90.61	DNA-dependent metalloprotease WSS1-like isoform X2 [Cucurbita pepo subsp. pepo] ...	[more]
XP_022151616.1	4.5e-170	89.12	DNA-dependent metalloprotease WSS1-like isoform X1 [Momordica charantia] >XP_022...	[more]

BLAST of Spg020028 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P38838	5.2e-28	37.85	DNA-dependent metalloprotease WSS1 OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
Q9P7B5	5.2e-12	32.65	DNA-dependent metalloprotease WSS1 homolog OS=Schizosaccharomyces pombe (strain ...	[more]
O94580	1.6e-08	28.21	DNA-dependent metalloprotease WSS1 homolog 2 OS=Schizosaccharomyces pombe (strai...	[more]

BLAST of Spg020028 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HT53	7.0e-177	89.47	DNA-dependent metalloprotease WSS1-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1HRV8	3.8e-175	91.52	DNA-dependent metalloprotease WSS1-like isoform X2 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1EKF1	2.5e-174	91.21	DNA-dependent metalloprotease WSS1-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1DF71	2.2e-170	89.12	DNA-dependent metalloprotease WSS1-like isoform X1 OS=Momordica charantia OX=367...	[more]
A0A1S4DV14	2.8e-165	88.15	LOW QUALITY PROTEIN: DNA-dependent metalloprotease WSS1-like OS=Cucumis melo OX=...	[more]

BLAST of Spg020028 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55915.1	4.0e-100	49.88	zinc ion binding	[more]
AT5G35690.1	4.1e-12	32.45	CONTAINS InterPro DOMAIN/s: WLM (InterPro:IPR013536), PUB domain (InterPro:IPR01...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001876	Zinc finger, RanBP2-type	SMART	SM00547	zf_4	coord: 396..420 e-value: 5.3E-4 score: 29.3 coord: 427..451 e-value: 0.11 score: 19.4
IPR001876	Zinc finger, RanBP2-type	PFAM	PF00641	zf-RanBP	coord: 397..422 e-value: 7.6E-6 score: 25.1
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS01358	ZF_RANBP2_1	coord: 398..417
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS01358	ZF_RANBP2_1	coord: 429..448
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS50199	ZF_RANBP2_2	coord: 423..454 score: 8.761258
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS50199	ZF_RANBP2_2	coord: 394..423 score: 8.919349
None	No IPR available	GENE3D	4.10.1060.10		coord: 391..428 e-value: 1.1E-6 score: 29.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 284..302
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 279..318
None	No IPR available	PANTHER	PTHR46622	DNA-DEPENDENT METALLOPROTEASE WSS1	coord: 392..468 coord: 140..359
None	No IPR available	PANTHER	PTHR46622:SF3	ZINC ION BINDING PROTEIN	coord: 392..468
None	No IPR available	PANTHER	PTHR46622:SF3	ZINC ION BINDING PROTEIN	coord: 140..359
None	No IPR available	CDD	cd07344	M48_yhfN_like	coord: 231..254 e-value: 4.64165E-5 score: 40.119
IPR013536	WLM domain	PFAM	PF08325	WLM	coord: 147..339 e-value: 2.9E-59 score: 200.3
IPR013536	WLM domain	PROSITE	PS51397	WLM	coord: 139..339 score: 45.542492
IPR036443	Zinc finger, RanBP2-type superfamily	SUPERFAMILY	90209	Ran binding protein zinc finger-like	coord: 394..421

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg020028.1	Spg020028.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008237	metallopeptidase activity