Spg019329 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg019329
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cytochrome P450
Location	scaffold1: 49461496 .. 49461867 (-)
RNA-Seq Expression	Spg019329
Synteny	Spg019329

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGATTTCCAAAGCTACATAATTCTCTCCCTCATATGTCTAGTTTCCACCATCACAGTTCGCTTCATTTTTGCCAAAACCAAACCAAACCCTCGTCTTCCTCCAAGCCCTTTTGCCCTTCCCATCATCGGCCATCTCCACCTCCTAGGAACATTGCCCCACAAAGCTTTCCACAAGCTCTCAGCCCGCCATGGCCCTCTAATGTACCTCCGTTTAGGCTCCATCCCCTGCATCGTAGTTTCGTCGGCCGAAACAGCGAAACAAATCCTCAAAACACAGGAATCATCCTTCTCCAACAGACCCCAATCGTCTGCAGTTGACTATCTAACCTACGACTCAGTGGATTTCGCCTTTGCTCCCTATGTGTAG

mRNA sequence

ATGGCGGATTTCCAAAGCTACATAATTCTCTCCCTCATATGTCTAGTTTCCACCATCACAGTTCGCTTCATTTTTGCCAAAACCAAACCAAACCCTCGTCTTCCTCCAAGCCCTTTTGCCCTTCCCATCATCGGCCATCTCCACCTCCTAGGAACATTGCCCCACAAAGCTTTCCACAAGCTCTCAGCCCGCCATGGCCCTCTAATGTACCTCCGTTTAGGCTCCATCCCCTGCATCGTAGTTTCGTCGGCCGAAACAGCGAAACAAATCCTCAAAACACAGGAATCATCCTTCTCCAACAGACCCCAATCGTCTGCAGTTGACTATCTAACCTACGACTCAGTGGATTTCGCCTTTGCTCCCTATGTGTAG

Coding sequence (CDS)

ATGGCGGATTTCCAAAGCTACATAATTCTCTCCCTCATATGTCTAGTTTCCACCATCACAGTTCGCTTCATTTTTGCCAAAACCAAACCAAACCCTCGTCTTCCTCCAAGCCCTTTTGCCCTTCCCATCATCGGCCATCTCCACCTCCTAGGAACATTGCCCCACAAAGCTTTCCACAAGCTCTCAGCCCGCCATGGCCCTCTAATGTACCTCCGTTTAGGCTCCATCCCCTGCATCGTAGTTTCGTCGGCCGAAACAGCGAAACAAATCCTCAAAACACAGGAATCATCCTTCTCCAACAGACCCCAATCGTCTGCAGTTGACTATCTAACCTACGACTCAGTGGATTTCGCCTTTGCTCCCTATGTGTAG

Protein sequence

MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFAPYV

Homology

[Close]

BLAST of Spg019329 vs. NCBI nr
Match: XP_022966275.1 (cytochrome P450 93A2 [Cucurbita maxima])

HSP 1 Score: 208.4 bits (529), Expect = 3.6e-50
Identity = 102/122 (83.61%), Postives = 112/122 (91.80%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M DFQSY IL LI L+STIT+RFIFAKTKPNPRLPP+P ALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LSARHGPL++LRLGS+PCIVVSSAETAKQ+LKT ESSFSNRP  +AVDYLTY+S DF+FA
Sbjct: 61  LSARHGPLIHLRLGSVPCIVVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYNSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. NCBI nr
Match: XP_023518782.1 (cytochrome P450 93A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 207.6 bits (527), Expect = 6.1e-50
Identity = 102/122 (83.61%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M DFQSY IL LI L+STIT+RFIFAKTKPNPRLPP+P ALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS RHGPLM+LRLGS+PCIVVSSAETAKQ+LKT ESSFSNRP  +AVDYLTY S DF+FA
Sbjct: 61  LSTRHGPLMHLRLGSVPCIVVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. NCBI nr
Match: KAG6595659.1 (3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 205.7 bits (522), Expect = 2.3e-49
Identity = 100/122 (81.97%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M DFQSY IL L+ L+STIT+RFIFAKTKPNPRLPP+P ALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MEDFQSYTILFLVWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS RHGPL++LRLGS+PCIVVSSAETAKQ+LKT ESSFSNRP  +AVDYLTY S DF+FA
Sbjct: 61  LSTRHGPLIHLRLGSVPCIVVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. NCBI nr
Match: XP_022925132.1 (cytochrome P450 93A2 [Cucurbita moschata])

HSP 1 Score: 205.7 bits (522), Expect = 2.3e-49
Identity = 100/122 (81.97%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M DFQSY IL LI L+STIT+RFIFAKTKPNPRLPP+P ALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS RHGPL++LRLGS+PCI+VSSAETAKQ+LKT ESSFSNRP  +AVDYLTY S DF+FA
Sbjct: 61  LSTRHGPLIHLRLGSVPCIIVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. NCBI nr
Match: KAG7027616.1 (3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 201.8 bits (512), Expect = 3.3e-48
Identity = 99/122 (81.15%), Postives = 109/122 (89.34%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M +FQSY IL LI L+STIT+RFIFAKTKPNPRLPP+P ALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS RHGPL++LRLGS+PCIVVSSAE AKQ+LKT ESSFSNRP  +AVDYLTY S DF+FA
Sbjct: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 7.3e-30
Identity = 69/114 (60.53%), Postives = 84/114 (73.68%), Query Frame = 0


Query: 12  LICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHGPLMYL 71
           LICLVSTI   +I  + +    LPPSP ALPIIGHLHL+  +PH+ F+KLS RHGP+M L
Sbjct: 7   LICLVSTIVFAYILWRKQSKKNLPPSPKALPIIGHLHLVSPIPHQDFYKLSTRHGPIMQL 66

Query: 72  RLGSIPCIVVSSAETAKQILKTQESSFSNRP-QSSAVDYLTYDSVD--FAFAPY 123
            LGS+PC+V S+AE AK+ LKT E +FSNRP Q+ AV  L YDS D  FAFAP+
Sbjct: 67  FLGSVPCVVASTAEAAKEFLKTHEINFSNRPGQNVAVKGLAYDSQDFLFAFAPF 120

[Close]

BLAST of Spg019329 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 6.8e-28
Identity = 65/113 (57.52%), Postives = 80/113 (70.80%), Query Frame = 0


Query: 13  ICLVSTITVRFIF--AKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHGPLMY 72
           ICL+STI    I    + K    LPPSP  LPIIGHLHLL   PH+ FHKLS R+GP+++
Sbjct: 8   ICLISTIVFASILWRKQNKNKTLLPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIH 67

Query: 73  LRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSS-AVDYLTYDSVDFAFAPY 123
           L LGS+PC+V S+AE AK+ LKT E +FSNRP ++ AV+ LTY   DF FAPY
Sbjct: 68  LFLGSVPCVVASTAEAAKEFLKTHEPAFSNRPANTVAVETLTYGFQDFLFAPY 120

[Close]

BLAST of Spg019329 vs. ExPASy Swiss-Prot
Match: Q42799 (Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.7e-26
Identity = 61/112 (54.46%), Postives = 80/112 (71.43%), Query Frame = 0


Query: 12  LICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHGPLMYL 71
           +IC+VS+I   +I  + +   +LPPSP  LPIIGHLHL+  +PH+ F+KLS RHGP+M L
Sbjct: 7   VICVVSSIVFAYIVWRKERKKKLPPSPKGLPIIGHLHLVSPIPHQDFYKLSLRHGPIMQL 66

Query: 72  RLGSIPCIVVSSAETAKQILKTQESSFSNRP-QSSAVDYLTYDSVDFAFAPY 123
            LGS+PC+V S+AE AK+ LKT E +FSNRP Q+ AV +LTY      F PY
Sbjct: 67  FLGSVPCVVASTAEAAKEFLKTHEINFSNRPGQNVAVQFLTY-----VFGPY 113

[Close]

BLAST of Spg019329 vs. ExPASy Swiss-Prot
Match: Q9XGT9 (Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-24
Identity = 55/98 (56.12%), Postives = 74/98 (75.51%), Query Frame = 0


Query: 25  FAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHGPLMYLRLGSIPCIVVSSA 84
           + + + + RLPPSP + PIIGHLH LG L H++FH LS R+G L++LRLGS+PC+VVS+ 
Sbjct: 24  YKRNQNHRRLPPSPPSFPIIGHLHHLGPLIHQSFHALSTRYGSLIHLRLGSVPCVVVSTP 83

Query: 85  ETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFAPY 123
           + AK  LKT E +FS+R  S A+D++TY  V FAFAPY
Sbjct: 84  DLAKDFLKTNELAFSSRKHSLAIDHITY-GVAFAFAPY 120

[Close]

BLAST of Spg019329 vs. ExPASy Swiss-Prot
Match: Q9XHC6 (Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 6.6e-23
Identity = 58/123 (47.15%), Postives = 82/123 (66.67%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPS-PFALPIIGHLHLLGTLPHKAFH 60
           M D + Y++L  +  +STI +R IF K +   RLPP  P ++P++GH   L +L H+A +
Sbjct: 1   MLDIKGYLVLFFLWFISTILIRSIFKKPQ-RLRLPPGPPISIPLLGHAPYLRSLLHQALY 60

Query: 61  KLSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAF 120
           KLS R+GPL+++ +GS   +V SSAETAKQILKT E +F NRP   A + LTY + D+ F
Sbjct: 61  KLSLRYGPLIHVMIGSKHVVVASSAETAKQILKTSEEAFCNRPLMIASESLTYGAADYFF 120

Query: 121 APY 123
            PY
Sbjct: 121 IPY 122

[Close]

BLAST of Spg019329 vs. ExPASy TrEMBL
Match: A0A6J1HR67 (cytochrome P450 93A2 OS=Cucurbita maxima OX=3661 GN=LOC111465987 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.7e-50
Identity = 102/122 (83.61%), Postives = 112/122 (91.80%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M DFQSY IL LI L+STIT+RFIFAKTKPNPRLPP+P ALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LSARHGPL++LRLGS+PCIVVSSAETAKQ+LKT ESSFSNRP  +AVDYLTY+S DF+FA
Sbjct: 61  LSARHGPLIHLRLGSVPCIVVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYNSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. ExPASy TrEMBL
Match: A0A6J1EAY9 (cytochrome P450 93A2 OS=Cucurbita moschata OX=3662 GN=LOC111432466 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-49
Identity = 100/122 (81.97%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M DFQSY IL LI L+STIT+RFIFAKTKPNPRLPP+P ALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS RHGPL++LRLGS+PCI+VSSAETAKQ+LKT ESSFSNRP  +AVDYLTY S DF+FA
Sbjct: 61  LSTRHGPLIHLRLGSVPCIIVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. ExPASy TrEMBL
Match: A0A0A0KW21 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G152790 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.3e-47
Identity = 98/122 (80.33%), Postives = 106/122 (86.89%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M DF+SYIIL  I LVST+T+R IFAK K N  LPPSPFALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MEDFESYIILFFIWLVSTLTIRLIFAKNKHNSHLPPSPFALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS RHGPLM+LRLGS+PC+VVSS ETAKQ+LKTQESSFSNRP  SAVDYLTY S DF+FA
Sbjct: 61  LSNRHGPLMHLRLGSVPCVVVSSPETAKQVLKTQESSFSNRPHLSAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. ExPASy TrEMBL
Match: A0A5A7UD61 (Cytochrome P450 93A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1192G00170 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.4e-46
Identity = 96/122 (78.69%), Postives = 105/122 (86.07%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           MADF SYIIL  I LVST+T+R +FAK K N  LPPSPFALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MADFGSYIILFFIWLVSTLTIRLLFAKNKLNSHLPPSPFALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS R+GPLM+LRLGS+PC+VVSS ETAKQ+LKT ESSFSNRP  SAVDYLTY S DF+FA
Sbjct: 61  LSTRYGPLMHLRLGSVPCVVVSSPETAKQVLKTHESSFSNRPHLSAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. ExPASy TrEMBL
Match: A0A5D3BM07 (Cytochrome P450 93A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2510G00380 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 4.4e-46
Identity = 96/122 (78.69%), Postives = 105/122 (86.07%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           MADF SYIIL  I LVST+T+R +FAK K N  LPPSPFALPIIGHLHLLG LPHKAFHK
Sbjct: 1   MADFGSYIILFFIWLVSTLTIRLLFAKNKLNSHLPPSPFALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS R+GPLM+LRLGS+PC+VVSS ETAKQ+LKT ESSFSNRP  SAVDYLTY S DF+FA
Sbjct: 61  LSTRYGPLMHLRLGSVPCVVVSSPETAKQVLKTHESSFSNRPHLSAVDYLTYGSADFSFA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. TAIR 10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1 )

HSP 1 Score: 133.7 bits (335), Expect = 1.0e-31
Identity = 67/122 (54.92%), Postives = 86/122 (70.49%), Query Frame = 0


Query: 1   MADFQSYIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHK 60
           M D Q + ++ L+CL  T+ ++ I  + +    LPPSP ALPIIGH+HLLG + H+A HK
Sbjct: 1   MVDLQYFSVIILVCLGITVLIQAITNRLRDRLPLPPSPTALPIIGHIHLLGPIAHQALHK 60

Query: 61  LSARHGPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFA 120
           LS R+GPLMYL +GSIP ++VSSAE A +ILK+ E +F NRP    VDYLTY S DF  A
Sbjct: 61  LSIRYGPLMYLFIGSIPNLIVSSAEMANEILKSNELNFLNRPTMQNVDYLTYGSADFFSA 120

Query: 121 PY 123
           PY
Sbjct: 121 PY 122

[Close]

BLAST of Spg019329 vs. TAIR 10
Match: AT5G42590.1 (cytochrome P450, family 71, subfamily A, polypeptide 16 )

HSP 1 Score: 105.5 bits (262), Expect = 3.0e-23
Identity = 52/117 (44.44%), Postives = 74/117 (63.25%), Query Frame = 0


Query: 8   IILSLICLVSTITVRFIFAK--TKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARH 67
           +IL  +CL + +T+   F     +PN  LPPSP+ LP+IG+LH L   PH+A   LSARH
Sbjct: 4   MILISLCLTTFLTILLFFKSLLKRPNSNLPPSPWRLPVIGNLHQLSLHPHRALSSLSARH 63

Query: 68  GPLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFAPY 123
           GPLM LR G +P ++VSSA+ A  ++KT +  F+NRP + +   ++    D  FAPY
Sbjct: 64  GPLMLLRFGRVPVLIVSSADVAHDVMKTHDLKFANRPITKSAHKISNGGRDLVFAPY 120

[Close]

BLAST of Spg019329 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 103.6 bits (257), Expect = 1.2e-22
Identity = 53/116 (45.69%), Postives = 78/116 (67.24%), Query Frame = 0


Query: 7   YIILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHG 66
           + ++SL+ +VS+I   F+         LPPSP +LPIIG+LH L  LPH+ FHKLS ++G
Sbjct: 7   FFLVSLLTIVSSI---FLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKYG 66

Query: 67  PLMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFAPY 123
           PL++LRLGS+P +V+SS+E A+ +LKT +    +RP++     L+Y   D  FAPY
Sbjct: 67  PLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPY 119

[Close]

BLAST of Spg019329 vs. TAIR 10
Match: AT2G30770.1 (cytochrome P450, family 71, subfamily A, polypeptide 13 )

HSP 1 Score: 101.3 bits (251), Expect = 5.7e-22
Identity = 52/115 (45.22%), Postives = 71/115 (61.74%), Query Frame = 0


Query: 8   IILSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHGP 67
           I L L  L++ + +R    +T     LPPSP+ LP+IG+LH L   PH++   LS R+GP
Sbjct: 13  ISLCLTTLITLLLLRRFLKRTATKVNLPPSPWRLPVIGNLHQLSLHPHRSLRSLSLRYGP 72

Query: 68  LMYLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFAPY 123
           LM L  G +P +VVSS E A+++LKT +  F+NRP+S AV  L     D  FAPY
Sbjct: 73  LMLLHFGRVPILVVSSGEAAQEVLKTHDHKFANRPRSKAVHGLMNGGRDVVFAPY 127

[Close]

BLAST of Spg019329 vs. TAIR 10
Match: AT2G02580.1 (cytochrome P450, family 71, subfamily B, polypeptide 9 )

HSP 1 Score: 100.5 bits (249), Expect = 9.8e-22
Identity = 51/113 (45.13%), Postives = 73/113 (64.60%), Query Frame = 0


Query: 10  LSLICLVSTITVRFIFAKTKPNPRLPPSPFALPIIGHLHLLGTLPHKAFHKLSARHGPLM 69
           LSL+ L   +   F   K + N + PPSP   PIIG+LH LG LPH++   LS  +GP+M
Sbjct: 7   LSLLFLCCILLAAFKHKKRRTNQQQPPSPPGFPIIGNLHQLGELPHQSLWSLSKTYGPVM 66

Query: 70  YLRLGSIPCIVVSSAETAKQILKTQESSFSNRPQSSAVDYLTYDSVDFAFAPY 123
            L+LGS+P +VVSS+ETAKQ+LK  +    +RP  +    L+Y+ +D AF+P+
Sbjct: 67  LLKLGSVPTVVVSSSETAKQVLKINDLHCCSRPSLAGAKELSYNYLDIAFSPF 119

The following BLAST results are available for this feature:

BLAST of Spg019329 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022966275.1	3.6e-50	83.61	cytochrome P450 93A2 [Cucurbita maxima]	[more]
XP_023518782.1	6.1e-50	83.61	cytochrome P450 93A2 [Cucurbita pepo subsp. pepo]	[more]
KAG6595659.1	2.3e-49	81.97	3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp...	[more]
XP_022925132.1	2.3e-49	81.97	cytochrome P450 93A2 [Cucurbita moschata]	[more]
KAG7027616.1	3.3e-48	81.15	3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Spg019329 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42798	7.3e-30	60.53	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1...	[more]
O81973	6.8e-28	57.52	Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1	[more]
Q42799	1.7e-26	54.46	Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1	[more]
Q9XGT9	2.1e-24	56.12	Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1	[more]
Q9XHC6	6.6e-23	47.15	Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1	[more]

BLAST of Spg019329 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HR67	1.7e-50	83.61	cytochrome P450 93A2 OS=Cucurbita maxima OX=3661 GN=LOC111465987 PE=3 SV=1	[more]
A0A6J1EAY9	1.1e-49	81.97	cytochrome P450 93A2 OS=Cucurbita moschata OX=3662 GN=LOC111432466 PE=3 SV=1	[more]
A0A0A0KW21	2.3e-47	80.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G152790 PE=3 SV=1	[more]
A0A5A7UD61	4.4e-46	78.69	Cytochrome P450 93A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A5D3BM07	4.4e-46	78.69	Cytochrome P450 93A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Spg019329 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06900.1	1.0e-31	54.92	cytochrome P450, family 93, subfamily D, polypeptide 1	[more]
AT5G42590.1	3.0e-23	44.44	cytochrome P450, family 71, subfamily A, polypeptide 16	[more]
AT1G13080.1	1.2e-22	45.69	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT2G30770.1	5.7e-22	45.22	cytochrome P450, family 71, subfamily A, polypeptide 13	[more]
AT2G02580.1	9.8e-22	45.13	cytochrome P450, family 71, subfamily B, polypeptide 9	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 86..107 score: 26.28 coord: 62..81 score: 32.05
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 20..122 e-value: 6.1E-22 score: 79.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 30..117
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 35..112 e-value: 4.2E-12 score: 45.6
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 5..122
None	No IPR available	PANTHER	PTHR24298:SF491	CYTOCHROME P450 93A2	coord: 5..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg019329.1	Spg019329.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen