Spg018432 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg018432
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionVacuolar protein 8
Locationscaffold3: 9580794 .. 9583159 (+)
RNA-Seq ExpressionSpg018432
SyntenySpg018432
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCGACGTGTGGACTTCTTCGGGTTGGACGTCTTCCACGTTTTGGACTTCGTCGGGTTGGACATCCACCACATGGACTTCTTCTGGGGGTTCACGTCGTCCTCACCCAGATGTTGAACAAGCAATGCGTCGATCGCTTAACGTCCCTCGTTGCATTTGTTGGACGTCTTCCACGTTGTGGACTTCGTCGAGTTGGACGTCCTCCACGTTTTGGACTTTGTCGGGTTGGGCGTCCACTATGTTTTTTACTTCTTCTGGGGGTTCACGTCGTCCTCACTAGACGTTGAACAAGCACTGCGTTGATCGCTTAACGTCCCTCATTGCATTCTTCCATGTCGTGGGCTTCGTCGGGTTGGACGTCCACCATGTTTTAGACTTCTTCTAGGAGTTCACGTCGTTCTCAACCAGATGTTGAACAAACAATGTGTCAATCGCTTAATGCATTTGCTCAACCTGCTCCAATTGAAATTTTTTTTGTTTTATTTGAATGAATTTTTGGAAAATCTTTCTAACCGTGGGGCCATTCTGGATGGTAAATGGGTGTAGTCTCTCTAGCTAAGGTGACTTCTTGGATGACCTCTTCCATTGGAAACTGAAAGGAAACTAGGAGGTGAGAGATGAAGTATCATCGTTGCTGTGGATGGATTATATAGATTATTGGAAAAACTCAATTTCATCAATCCCTAAACTAAAATTTGAGTCCAAATTTGATTTTTTTTAAAATCCAAATCTCAAAATTGGGATTAAACATCCTCCACCCATTTTCACGAGAAAATACTGACCACCGAAAAATTTCTTCTCGCCTCCATGGTTTCCGACTGCTATTTCTTCCATGTCTACTTATATAGTTCATCAATTCCGTCAAATTTCAAGCTGCTTGCTCATTGTTCTCGTCTCTTCATCTAATTGGACGCTTTGAATTTCGTCGGTTTTCATTTTCTAACATGGGCCACTCACAGATTCAGAGAGAATCTGCAAAGCCGAGTTTAGATTGGGAAGCAGCGCTGAAGCGGTATGAAAACGTTATTGCTTCAGAATCCGAGGCTGTTAAAGTGAAAGCCACAGTCAAATTGGCCCATCTCTCCAAAAAGGCCCCCGAGAATATTTTGAACAGCACCATACTCATTATTGCTAAGCATCTTGAGGGTAACCTCATCGATAATTCAAGCCATTCCATGAAAGGAGTTGCTGCATATTGTCTAAGATGTATATCTTGTCAAGGTGATGGTACTTTGGCGACGGCAGTAGATCATTCTGGCGCTCTACAGTCGTTGCTGAGGTTATTGTCACATTCTAGAGGTTGTTTTCGTAAGATTTTAGTTAAATGTGTTTGGAGTATTGTCACTTTTGATAAAACTAGTTGTGTAATTATAGCAAGAAATGGGGGTTTGGAAGTTATTATTGGCATGTTTGATTCTGTAATTGATGGCACTAAGAGATATTTATTGGAGATTTTGAGTGCAATGGCACTATTGAGAGAGGTTAGGAAGGCCCTTATTAGTTTAAGAGGACTCCCTTTTCTTGTGCAAGCTGCTAGATGTGGTTGCATGGCCTCCAGAGAAAGAGCTTGCCAAGCAATTGGGTTGCTTGCAATTTCCAAGCGTGGAAGACACATGCTTGTTGAGTTGGGAGTGGTTTCAGTGCTTATTGAACTATTTCGTGAAGGAGATTGTGTGACAAAACTGGTAGCTGGTAATGCTCTAGGAATAGTTTCAGCTCATGTAGCCTATATTAGGCCTGTTGCACAAGGTGGGGTCATTCCTTTATTTGCTGATCTTCTTCAGTGCCCTGACCTTACTGGTAAGGAGATTGCAGAAGATGTCTTCTGTCTCTTAGCTGTTGCTGAAGCGAATGCAGTAGTCATTTCTGATCATCTAGTGAGAATTCTTAAAGAAGGTGATGATGGAGCAAAGGCTGCAGCTGCTGATGTTTTATGGGATCTTTCAGGCTATAAGTCCTCCATATCAGTTGTGCATAGTTCGGGTGCCATTCCAGTTTTGGTGGATCTATTACTGGTCGGGAATGATGAAGTGAGGGAAAAATTCTCTAGAGCTATAACCCAATTAAGTTATAATGAGACAGACAGGGCTGCACTTGCTGATGCAGGAGCGGTTGAAATACTAACAGGCCAAGGCTGTTGCAAGATGAGTTAGAAGAACTGAAAGATAATTCTGCTGAGGCCCTTATAAATTTTTCTGAAGACCCCTTATACTATGATAGAGTATCTGAAGCAGTTAACACTCCTGCTTTCCAGAATATGCAGGAAAGAATGACCCATATTCATGCTACTGAAAGGTACACCACAAGGTCCCTACGTCAAATGGGAATCGACCAACTTACCAGGGATCCAGATCTTCTATAA

mRNA sequence

ATGTCCTCGACGTGTGGACTTCTTCGGGTTGGACGTCTTCCACGTTTTGGACTTCGTCGGGTTGGACATCCACCACATGGACTTCTTCTGGGGGTTCACGTCGTCCTCACCCAGATGTTGAACAAGCAATGCGTCGATCGCTTAACGTCCCTCGTTGCATTTGTTGGACGTCTTCCACGTTGTGGACTTCGTCGAGTTGGACGTCCTCCACGTTTTGGACTTTGTCGGGTTGGGCGTCCACTATTCTCTCTAGCTAAGTTCATCAATTCCGTCAAATTTCAAGCTGCTTGCTCATTGTTCTCGTCTCTTCATCTAATTGGACGCTTTGAATTTCGTCGGTTTTCATTTTCTAACATGGGCCACTCACAGATTCAGAGAGAATCTGCAAAGCCGAGTTTAGATTGGGAAGCAGCGCTGAAGCGGTATGAAAACGTTATTGCTTCAGAATCCGAGGCTGTTAAAGTGAAAGCCACAGTCAAATTGGCCCATCTCTCCAAAAAGGCCCCCGAGAATATTTTGAACAGCACCATACTCATTATTGCTAAGCATCTTGAGGGTAACCTCATCGATAATTCAAGCCATTCCATGAAAGGAGTTGCTGCATATTGTCTAAGATGTATATCTTGTCAAGGTGATGGTACTTTGGCGACGGCAGTAGATCATTCTGGCGCTCTACAGTCGTTGCTGAGGTTATTGTCACATTCTAGAGGTTGTTTTCGTAAGATTTTAGTTAAATGTGTTTGGAGTATTGTCACTTTTGATAAAACTAGTTGTGTAATTATAGCAAGAAATGGGGGTTTGGAAGTTATTATTGGCATGTTTGATTCTGTAATTGATGGCACTAAGAGATATTTATTGGAGATTTTGAGTGCAATGGCACTATTGAGAGAGGTTAGGAAGGCCCTTATTAGTTTAAGAGGACTCCCTTTTCTTGTGCAAGCTGCTAGATGTGGTTGCATGGCCTCCAGAGAAAGAGCTTGCCAAGCAATTGGGTTGCTTGCAATTTCCAAGCGTGGAAGACACATGCTTGTTGAGTTGGGAGTGGTTTCAGTGCTTATTGAACTATTTCGTGAAGGAGATTGTGTGACAAAACTGGTAGCTGGTAATGCTCTAGGAATAGTTTCAGCTCATGTAGCCTATATTAGGCCTGTTGCACAAGGTGGGGTCATTCCTTTATTTGCTGATCTTCTTCAGTGCCCTGACCTTACTGGTAAGGAGATTGCAGAAGATGTCTTCTGTCTCTTAGCTGTTGCTGAAGCGAATGCAGTAGTCATTTCTGATCATCTAGTGAGAATTCTTAAAGAAGGTGATGATGGAGCAAAGGCTGCAGCTGCTGATGTTTTATGGGATCTTTCAGGCTATAAGTCCTCCATATCAGTTGTGCATAGTTCGGGTGCCATTCCAGTTTTGGTGGATCTATTACTGGTCGGGAATGATGAAGTGAGGGAAAAATTCTCTAGAGCTATAACCCAATTAAGTTATAATGAGACAGACAGGGCTGCACTTGCTGATGCAGGAGCGGTTGAAATACTAACAGGCCAAGGCTACCCCTTATACTATGATAGAGTATCTGAAGCAGTTAACACTCCTGCTTTCCAGAATATGCAGGAAAGAATGACCCATATTCATGCTACTGAAAGGTACACCACAAGGTCCCTACGTCAAATGGGAATCGACCAACTTACCAGGGATCCAGATCTTCTATAA

Coding sequence (CDS)

ATGTCCTCGACGTGTGGACTTCTTCGGGTTGGACGTCTTCCACGTTTTGGACTTCGTCGGGTTGGACATCCACCACATGGACTTCTTCTGGGGGTTCACGTCGTCCTCACCCAGATGTTGAACAAGCAATGCGTCGATCGCTTAACGTCCCTCGTTGCATTTGTTGGACGTCTTCCACGTTGTGGACTTCGTCGAGTTGGACGTCCTCCACGTTTTGGACTTTGTCGGGTTGGGCGTCCACTATTCTCTCTAGCTAAGTTCATCAATTCCGTCAAATTTCAAGCTGCTTGCTCATTGTTCTCGTCTCTTCATCTAATTGGACGCTTTGAATTTCGTCGGTTTTCATTTTCTAACATGGGCCACTCACAGATTCAGAGAGAATCTGCAAAGCCGAGTTTAGATTGGGAAGCAGCGCTGAAGCGGTATGAAAACGTTATTGCTTCAGAATCCGAGGCTGTTAAAGTGAAAGCCACAGTCAAATTGGCCCATCTCTCCAAAAAGGCCCCCGAGAATATTTTGAACAGCACCATACTCATTATTGCTAAGCATCTTGAGGGTAACCTCATCGATAATTCAAGCCATTCCATGAAAGGAGTTGCTGCATATTGTCTAAGATGTATATCTTGTCAAGGTGATGGTACTTTGGCGACGGCAGTAGATCATTCTGGCGCTCTACAGTCGTTGCTGAGGTTATTGTCACATTCTAGAGGTTGTTTTCGTAAGATTTTAGTTAAATGTGTTTGGAGTATTGTCACTTTTGATAAAACTAGTTGTGTAATTATAGCAAGAAATGGGGGTTTGGAAGTTATTATTGGCATGTTTGATTCTGTAATTGATGGCACTAAGAGATATTTATTGGAGATTTTGAGTGCAATGGCACTATTGAGAGAGGTTAGGAAGGCCCTTATTAGTTTAAGAGGACTCCCTTTTCTTGTGCAAGCTGCTAGATGTGGTTGCATGGCCTCCAGAGAAAGAGCTTGCCAAGCAATTGGGTTGCTTGCAATTTCCAAGCGTGGAAGACACATGCTTGTTGAGTTGGGAGTGGTTTCAGTGCTTATTGAACTATTTCGTGAAGGAGATTGTGTGACAAAACTGGTAGCTGGTAATGCTCTAGGAATAGTTTCAGCTCATGTAGCCTATATTAGGCCTGTTGCACAAGGTGGGGTCATTCCTTTATTTGCTGATCTTCTTCAGTGCCCTGACCTTACTGGTAAGGAGATTGCAGAAGATGTCTTCTGTCTCTTAGCTGTTGCTGAAGCGAATGCAGTAGTCATTTCTGATCATCTAGTGAGAATTCTTAAAGAAGGTGATGATGGAGCAAAGGCTGCAGCTGCTGATGTTTTATGGGATCTTTCAGGCTATAAGTCCTCCATATCAGTTGTGCATAGTTCGGGTGCCATTCCAGTTTTGGTGGATCTATTACTGGTCGGGAATGATGAAGTGAGGGAAAAATTCTCTAGAGCTATAACCCAATTAAGTTATAATGAGACAGACAGGGCTGCACTTGCTGATGCAGGAGCGGTTGAAATACTAACAGGCCAAGGCTACCCCTTATACTATGATAGAGTATCTGAAGCAGTTAACACTCCTGCTTTCCAGAATATGCAGGAAAGAATGACCCATATTCATGCTACTGAAAGGTACACCACAAGGTCCCTACGTCAAATGGGAATCGACCAACTTACCAGGGATCCAGATCTTCTATAA

Protein sequence

MSSTCGLLRVGRLPRFGLRRVGHPPHGLLLGVHVVLTQMLNKQCVDRLTSLVAFVGRLPRCGLRRVGRPPRFGLCRVGRPLFSLAKFINSVKFQAACSLFSSLHLIGRFEFRRFSFSNMGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTILIIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGCFRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREVRKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFREGDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETDRAALADAGAVEILTGQGYPLYYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL
Homology
BLAST of Spg018432 vs. NCBI nr
Match: XP_038897634.1 (protein spotted leaf 11 [Benincasa hispida] >XP_038897635.1 protein spotted leaf 11 [Benincasa hispida])

HSP 1 Score: 683.7 bits (1763), Expect = 1.3e-192
Identity = 372/470 (79.15%), Postives = 392/470 (83.40%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           MGHSQI R S KP+LDWEAAL +YENV+ASESEAVKVKAT+KLAHLSK APENILNS I 
Sbjct: 1   MGHSQIHRASEKPNLDWEAALTQYENVMASESEAVKVKATIKLAHLSKNAPENILNSAIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
            IAKHLE N   NSS SM+G AAYCLRCISCQGDGTLA AV HSGAL+SLLR L HS GC
Sbjct: 61  FIAKHLEDNPTSNSSQSMRGAAAYCLRCISCQGDGTLAAAVGHSGALESLLRSLPHSSGC 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           FRKILVKCVWSIVTFD+TS VIIARNGGLEVIIGMFDSV DGT+RYLLEILSAMALLREV
Sbjct: 121 FRKILVKCVWSIVTFDQTSRVIIARNGGLEVIIGMFDSVFDGTRRYLLEILSAMALLREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKALISLRGLPFLVQAAR GCMASRERACQAIGLLAI+KRGR MLVELGVV VLIEL RE
Sbjct: 181 RKALISLRGLPFLVQAARFGCMASRERACQAIGLLAITKRGRRMLVELGVVPVLIELLRE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLVAGN+LG+VSAHV YIRP+AQ G IPLFA+LLQ PD  GKEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVAGNSLGVVSAHVTYIRPLAQAGAIPLFAELLQWPDPIGKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           EANAVVISDHLVRILKEGD G KAAAADVLWDLS YK SISVV +SGAIPVLVDLL  GN
Sbjct: 301 EANAVVISDHLVRILKEGDSGGKAAAADVLWDLSSYKYSISVVQNSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVEILTG--------------------QGYPL 538
           DEVREK S AI QLSYNETDR ALADAGA++ L G                       PL
Sbjct: 361 DEVREKVSGAIAQLSYNETDRVALADAGAIQRLIGLLQDELEEVKDNVTEALLNFSEDPL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           Y  RVSEAV+TPAFQN+QERM HI A ER + RSL QMGI+Q T DPDLL
Sbjct: 421 YCTRVSEAVSTPAFQNLQERMIHIRAAERQSVRSLHQMGINQFTGDPDLL 470

BLAST of Spg018432 vs. NCBI nr
Match: XP_022936629.1 (uncharacterized protein LOC111443172 [Cucurbita moschata])

HSP 1 Score: 643.7 bits (1659), Expect = 1.5e-180
Identity = 353/470 (75.11%), Postives = 387/470 (82.34%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           MGHSQI R S KP+LDWEAALK+YENV+A+ESEAVKVKAT+KLA LS  AP NILNSTI 
Sbjct: 1   MGHSQIHRASEKPNLDWEAALKQYENVMATESEAVKVKATIKLASLSNDAPHNILNSTIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
           IIAKHLE N I NSS SM+G AAYCL+ IS QGDGTLATAV HSGAL+S+LR L  + G 
Sbjct: 61  IIAKHLEVNPISNSSQSMRGAAAYCLKRISRQGDGTLATAVAHSGALESILRSLPDTSGG 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           FRKILVKC+WSIVT DK S VI+ARNGGLEV+IGMFDSV+DGT+RYLLEILSA+AL+REV
Sbjct: 121 FRKILVKCIWSIVTCDKPSRVIVARNGGLEVVIGMFDSVVDGTRRYLLEILSALALVREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKALISLRGLPFLVQAAR GCMASRERACQAIGL+AI++RGRHMLVELGV+ VLIELF E
Sbjct: 181 RKALISLRGLPFLVQAARYGCMASRERACQAIGLIAITRRGRHMLVELGVIPVLIELFCE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLVAGN+LGIVSAHVAYIRPVAQ G IPLFADLLQ P+   KEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVAGNSLGIVSAHVAYIRPVAQAGAIPLFADLLQWPNPIAKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           EANAV+I DHLVRILKEGDD  KAAAADVLWDLS YK SI +V SSGAIPVLVDLL  GN
Sbjct: 301 EANAVLIFDHLVRILKEGDDEGKAAAADVLWDLSSYKYSIPIVQSSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVEILTG--------------------QGYPL 538
            EVREK S A+ QLSY+E DR ALADAGA+  L G                        L
Sbjct: 361 GEVREKVSGAVAQLSYSEADRIALADAGAIHGLIGLLQDELDEVKDNAAEALINFSEDAL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           Y +RVSEA++ PAFQN+ ERMTHI ATER+  RSLRQMGI+QLT DPDLL
Sbjct: 421 YCNRVSEAISNPAFQNLLERMTHIRATERHGVRSLRQMGINQLTGDPDLL 470

BLAST of Spg018432 vs. NCBI nr
Match: XP_022139563.1 (uncharacterized protein LOC111010437 [Momordica charantia])

HSP 1 Score: 642.9 bits (1657), Expect = 2.6e-180
Identity = 359/471 (76.22%), Postives = 394/471 (83.65%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           M  S+I++ES KP+L+WE AL++Y+NV+ASESEAVKVKATVKLA+LS+ APENILNSTI 
Sbjct: 1   MSCSRIRKESEKPNLNWEEALRQYQNVMASESEAVKVKATVKLANLSQCAPENILNSTIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQG-DGTLATAVDHSGALQSLLRLLSHSRG 238
           IIAKHL  N I NSS SM+G AAYCLR ISC+G DGTLA AV +SGAL+SLLRLL +S G
Sbjct: 61  IIAKHLGDNPITNSSQSMQGAAAYCLRRISCRGDDGTLAMAVGNSGALESLLRLLPYSNG 120

Query: 239 CFRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLRE 298
            FRKILVKCVWS+VTF KTS VIIARNGGLEVIIGM DSVID ++RYLLEILSA+ALLRE
Sbjct: 121 GFRKILVKCVWSVVTFGKTSRVIIARNGGLEVIIGMLDSVIDASRRYLLEILSALALLRE 180

Query: 299 VRKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFR 358
           VRKALISLRGLPFLV+AARCGC+ASRERACQAIGLLAI+KRGR ML ELGVV VLIELFR
Sbjct: 181 VRKALISLRGLPFLVEAARCGCLASRERACQAIGLLAITKRGRRMLFELGVVPVLIELFR 240

Query: 359 EGDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPD-LTGKEIAEDVFCLLA 418
            GDC TKLVAGN+LGIVSAHVAYIRPVAQ G IPLFADLLQ P+   GKEIAEDVFCLLA
Sbjct: 241 GGDCTTKLVAGNSLGIVSAHVAYIRPVAQAGAIPLFADLLQWPNHPIGKEIAEDVFCLLA 300

Query: 419 VAEANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLV 478
           VAE NAVVISDHLVRILKEGDD AKAAAADVLWDLSGYK SIS  H+SGAIPV+VDLL  
Sbjct: 301 VAEENAVVISDHLVRILKEGDDEAKAAAADVLWDLSGYKYSISTAHNSGAIPVMVDLLQD 360

Query: 479 GNDEVREKFSRAITQLSYNETDRAALADAGAV-------------------EILTGQGYP 538
            N+EVREK S AI QLSYNE DRAALADAGA+                   E L      
Sbjct: 361 RNNEVREKVSGAIAQLSYNEIDRAALADAGAIERLIELLQDELEELKDNAAEALINFSED 420

Query: 539 LYY-DRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDL 568
           L+Y  RVSEAV+TPAF+NMQERMTHI  TE+++ RSLRQMGIDQLTRDPDL
Sbjct: 421 LFYCGRVSEAVSTPAFRNMQERMTHIRTTEQHSMRSLRQMGIDQLTRDPDL 471

BLAST of Spg018432 vs. NCBI nr
Match: KAG6592081.1 (U-box domain-containing protein 12, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024957.1 Armadillo repeat-containing protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 642.5 bits (1656), Expect = 3.4e-180
Identity = 352/470 (74.89%), Postives = 387/470 (82.34%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           MGHSQI R S KP+LDWEAALK+YENV+A+ESEAVKVKAT+KLA LS  AP NILNSTI 
Sbjct: 1   MGHSQIHRASEKPNLDWEAALKQYENVMATESEAVKVKATIKLASLSNDAPHNILNSTIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
           IIAKHLE N I NSS SM+G AAYCL+ IS QGDGTLATAV HSGAL+S+LR L  + G 
Sbjct: 61  IIAKHLEVNPISNSSQSMRGAAAYCLKRISRQGDGTLATAVAHSGALESILRSLPDTSGG 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           FRKILVKC+WSIVT DK S VI+ARNGGLEV+IGMFDSV+DGT+RYLLEILSA+AL+REV
Sbjct: 121 FRKILVKCIWSIVTCDKPSRVIVARNGGLEVVIGMFDSVVDGTRRYLLEILSALALVREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKALISLRGLPFLVQAAR GCMASRERACQAIGL+AI++RGRHMLVELGV+ VLIELF E
Sbjct: 181 RKALISLRGLPFLVQAARYGCMASRERACQAIGLIAITRRGRHMLVELGVIPVLIELFCE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLV+GN+LGIVSAHVAYIRPVAQ G IPLFADLLQ P+   KEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVSGNSLGIVSAHVAYIRPVAQAGAIPLFADLLQWPNPIAKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           EANAV+I DHLVRILKEGDD  KAAAADVLWDLS YK SI +V SSGAIPVLVDLL  GN
Sbjct: 301 EANAVLIFDHLVRILKEGDDEGKAAAADVLWDLSSYKYSIPIVQSSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVEILTG--------------------QGYPL 538
            EVREK S A+ QLSY+E DR ALADAGA+  L G                        L
Sbjct: 361 GEVREKVSGAVAQLSYSEADRIALADAGAIHGLIGLLQDELDEVKDNAAEALINFSEDAL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           Y +RVSEA++ PAFQN+ ERMTHI ATER+  RSLRQMGI+QLT DPDLL
Sbjct: 421 YCNRVSEAISNPAFQNLLERMTHIRATERHGVRSLRQMGINQLTGDPDLL 470

BLAST of Spg018432 vs. NCBI nr
Match: XP_022976328.1 (uncharacterized protein LOC111476761 [Cucurbita maxima])

HSP 1 Score: 640.2 bits (1650), Expect = 1.7e-179
Identity = 349/470 (74.26%), Postives = 387/470 (82.34%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           M HS+I+R S KP+LDWEAALK+YENV+A+ESEAVKVKAT+KLA LS  AP NILNSTI 
Sbjct: 1   MSHSEIRRASEKPNLDWEAALKQYENVMATESEAVKVKATIKLASLSNDAPHNILNSTIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
           IIAKHLE N I NSS SM+G AAYCL+ IS QGDGTLATA+ HSGAL+S+LR L  + G 
Sbjct: 61  IIAKHLEVNPISNSSQSMRGAAAYCLKRISRQGDGTLATAIAHSGALESILRSLPDTSGG 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           FRKILVKC+WSIVT DK S VI+ARNGGLEV+IGMFDSV+DGT+RYLLEILSA+AL+REV
Sbjct: 121 FRKILVKCIWSIVTCDKHSRVIVARNGGLEVVIGMFDSVVDGTRRYLLEILSALALVREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKALISLRGLPFLVQAAR GCMASRERACQAIGL+AI++RGRHMLVELGV+ VLIELF E
Sbjct: 181 RKALISLRGLPFLVQAARYGCMASRERACQAIGLIAITRRGRHMLVELGVIPVLIELFCE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLVAGN+LGIVSAHVAYIRPVAQ G IPLFADLLQ PD   KEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVAGNSLGIVSAHVAYIRPVAQAGAIPLFADLLQWPDPIAKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           EANAV+I DHLVRILKEGDD  KAAAADVLWDLS YK SI +V SSGAIPVLVDLL  GN
Sbjct: 301 EANAVLIFDHLVRILKEGDDEGKAAAADVLWDLSSYKYSIPIVQSSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVE--------------------ILTGQGYPL 538
            EVREK S A+ QLSY+E DR ALADAGA+                     ++      L
Sbjct: 361 GEVREKVSGAVAQLSYSEADRIALADAGAIHGLIELLQDELDEVKDNAAEALINFSEDAL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           Y +RVSEA++ PAFQN+ ERMTHI ATER+  RSLRQMGI+QLT DPDLL
Sbjct: 421 YCNRVSEAISNPAFQNLLERMTHIRATERHGVRSLRQMGINQLTGDPDLL 470

BLAST of Spg018432 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 6.2e-07
Identity = 42/133 (31.58%), Postives = 64/133 (48.12%), Query Frame = 0

Query: 384 VAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEANAVVISD-----HLVRILKEGDD 443
           +A  G IPL   LL  PD   +E A      L++ E N  +IS+     +++ IL+ G+ 
Sbjct: 417 IANAGAIPLLVQLLSYPDSGIQENAVTTLLNLSIDEVNKKLISNEGAIPNIIEILENGNR 476

Query: 444 GAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETD 503
            A+  +A  L+ LS    +   +  S  IP LVDLL  G    ++    A+  LS N  +
Sbjct: 477 EARENSAAALFSLSMLDENKVTIGLSNGIPPLVDLLQHGTLRGKKDALTALFNLSLNSAN 536

Query: 504 RAALADAGAVEIL 512
           +    DAG V+ L
Sbjct: 537 KGRAIDAGIVQPL 549

BLAST of Spg018432 vs. ExPASy Swiss-Prot
Match: A2ZLU6 (Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 2.0e-05
Identity = 42/135 (31.11%), Postives = 63/135 (46.67%), Query Frame = 0

Query: 384 VAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEAN-AVVISD----HLVRILKEGDD 443
           +A+ G IPL   LL   DL  +E A      L++ E N A +IS      +V +LK G  
Sbjct: 405 IAEAGAIPLLLSLLSSSDLRTQEHAVTALLNLSIHEDNKASIISSGAVPSIVHVLKNGSM 464

Query: 444 GAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETD 503
            A+  AA  L+ LS        +   GAIP LV LL  G+   ++  + A+  L   + +
Sbjct: 465 EARENAAATLFSLSVIDEYKVTIGGMGAIPALVVLLGEGSQRGKKDAAAALFNLCIYQGN 524

Query: 504 RAALADAGAVEILTG 514
           +     AG V ++ G
Sbjct: 525 KGRAIRAGLVPLIMG 539

BLAST of Spg018432 vs. ExPASy Swiss-Prot
Match: Q0IMG9 (E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 2.0e-05
Identity = 42/135 (31.11%), Postives = 63/135 (46.67%), Query Frame = 0

Query: 384 VAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEAN-AVVISD----HLVRILKEGDD 443
           +A+ G IPL   LL   DL  +E A      L++ E N A +IS      +V +LK G  
Sbjct: 405 IAEAGAIPLLLSLLSSSDLRTQEHAVTALLNLSIHEDNKASIISSGAVPSIVHVLKNGSM 464

Query: 444 GAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETD 503
            A+  AA  L+ LS        +   GAIP LV LL  G+   ++  + A+  L   + +
Sbjct: 465 EARENAAATLFSLSVIDEYKVTIGGMGAIPALVVLLGEGSQRGKKDAAAALFNLCIYQGN 524

Query: 504 RAALADAGAVEILTG 514
           +     AG V ++ G
Sbjct: 525 KGRAIRAGLVPLIMG 539

BLAST of Spg018432 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 5.8e-05
Identity = 39/133 (29.32%), Postives = 63/133 (47.37%), Query Frame = 0

Query: 384 VAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEANAVVIS-----DHLVRILKEGDD 443
           +A+ G IP+  +LL   D+  +E A      L++ E N  +I        +V++L+ G  
Sbjct: 369 IAEAGAIPVLVNLLTSEDVATQENAITCVLNLSIYENNKELIMFAGAVTSIVQVLRAGTM 428

Query: 444 GAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETD 503
            A+  AA  L+ LS    +  ++  SGAIP LVDLL  G    ++  + A+  L     +
Sbjct: 429 EARENAAATLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAATALFNLCIYHGN 488

Query: 504 RAALADAGAVEIL 512
           +     AG V  L
Sbjct: 489 KGRAVRAGIVTAL 501

BLAST of Spg018432 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 5.8e-05
Identity = 40/134 (29.85%), Postives = 65/134 (48.51%), Query Frame = 0

Query: 384 VAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEAN--AVVIS---DHLVRILKEGDD 443
           +A+ G IPL   LL  PD   +E +      L++ E N  A+V +     +V++LK+G  
Sbjct: 390 IAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSICENNKGAIVSAGAIPGIVQVLKKGSM 449

Query: 444 GAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETD 503
            A+  AA  L+ LS    +   + + GAIP LV LL  G    ++  + A+  L   + +
Sbjct: 450 EARENAAATLFSLSVIDENKVTIGALGAIPPLVVLLNEGTQRGKKDAATALFNLCIYQGN 509

Query: 504 RAALADAGAVEILT 513
           +     AG +  LT
Sbjct: 510 KGKAIRAGVIPTLT 523

BLAST of Spg018432 vs. ExPASy TrEMBL
Match: A0A6J1F8U2 (uncharacterized protein LOC111443172 OS=Cucurbita moschata OX=3662 GN=LOC111443172 PE=4 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 7.4e-181
Identity = 353/470 (75.11%), Postives = 387/470 (82.34%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           MGHSQI R S KP+LDWEAALK+YENV+A+ESEAVKVKAT+KLA LS  AP NILNSTI 
Sbjct: 1   MGHSQIHRASEKPNLDWEAALKQYENVMATESEAVKVKATIKLASLSNDAPHNILNSTIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
           IIAKHLE N I NSS SM+G AAYCL+ IS QGDGTLATAV HSGAL+S+LR L  + G 
Sbjct: 61  IIAKHLEVNPISNSSQSMRGAAAYCLKRISRQGDGTLATAVAHSGALESILRSLPDTSGG 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           FRKILVKC+WSIVT DK S VI+ARNGGLEV+IGMFDSV+DGT+RYLLEILSA+AL+REV
Sbjct: 121 FRKILVKCIWSIVTCDKPSRVIVARNGGLEVVIGMFDSVVDGTRRYLLEILSALALVREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKALISLRGLPFLVQAAR GCMASRERACQAIGL+AI++RGRHMLVELGV+ VLIELF E
Sbjct: 181 RKALISLRGLPFLVQAARYGCMASRERACQAIGLIAITRRGRHMLVELGVIPVLIELFCE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLVAGN+LGIVSAHVAYIRPVAQ G IPLFADLLQ P+   KEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVAGNSLGIVSAHVAYIRPVAQAGAIPLFADLLQWPNPIAKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           EANAV+I DHLVRILKEGDD  KAAAADVLWDLS YK SI +V SSGAIPVLVDLL  GN
Sbjct: 301 EANAVLIFDHLVRILKEGDDEGKAAAADVLWDLSSYKYSIPIVQSSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVEILTG--------------------QGYPL 538
            EVREK S A+ QLSY+E DR ALADAGA+  L G                        L
Sbjct: 361 GEVREKVSGAVAQLSYSEADRIALADAGAIHGLIGLLQDELDEVKDNAAEALINFSEDAL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           Y +RVSEA++ PAFQN+ ERMTHI ATER+  RSLRQMGI+QLT DPDLL
Sbjct: 421 YCNRVSEAISNPAFQNLLERMTHIRATERHGVRSLRQMGINQLTGDPDLL 470

BLAST of Spg018432 vs. ExPASy TrEMBL
Match: A0A6J1CFX3 (uncharacterized protein LOC111010437 OS=Momordica charantia OX=3673 GN=LOC111010437 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 1.3e-180
Identity = 359/471 (76.22%), Postives = 394/471 (83.65%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           M  S+I++ES KP+L+WE AL++Y+NV+ASESEAVKVKATVKLA+LS+ APENILNSTI 
Sbjct: 1   MSCSRIRKESEKPNLNWEEALRQYQNVMASESEAVKVKATVKLANLSQCAPENILNSTIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQG-DGTLATAVDHSGALQSLLRLLSHSRG 238
           IIAKHL  N I NSS SM+G AAYCLR ISC+G DGTLA AV +SGAL+SLLRLL +S G
Sbjct: 61  IIAKHLGDNPITNSSQSMQGAAAYCLRRISCRGDDGTLAMAVGNSGALESLLRLLPYSNG 120

Query: 239 CFRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLRE 298
            FRKILVKCVWS+VTF KTS VIIARNGGLEVIIGM DSVID ++RYLLEILSA+ALLRE
Sbjct: 121 GFRKILVKCVWSVVTFGKTSRVIIARNGGLEVIIGMLDSVIDASRRYLLEILSALALLRE 180

Query: 299 VRKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFR 358
           VRKALISLRGLPFLV+AARCGC+ASRERACQAIGLLAI+KRGR ML ELGVV VLIELFR
Sbjct: 181 VRKALISLRGLPFLVEAARCGCLASRERACQAIGLLAITKRGRRMLFELGVVPVLIELFR 240

Query: 359 EGDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPD-LTGKEIAEDVFCLLA 418
            GDC TKLVAGN+LGIVSAHVAYIRPVAQ G IPLFADLLQ P+   GKEIAEDVFCLLA
Sbjct: 241 GGDCTTKLVAGNSLGIVSAHVAYIRPVAQAGAIPLFADLLQWPNHPIGKEIAEDVFCLLA 300

Query: 419 VAEANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLV 478
           VAE NAVVISDHLVRILKEGDD AKAAAADVLWDLSGYK SIS  H+SGAIPV+VDLL  
Sbjct: 301 VAEENAVVISDHLVRILKEGDDEAKAAAADVLWDLSGYKYSISTAHNSGAIPVMVDLLQD 360

Query: 479 GNDEVREKFSRAITQLSYNETDRAALADAGAV-------------------EILTGQGYP 538
            N+EVREK S AI QLSYNE DRAALADAGA+                   E L      
Sbjct: 361 RNNEVREKVSGAIAQLSYNEIDRAALADAGAIERLIELLQDELEELKDNAAEALINFSED 420

Query: 539 LYY-DRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDL 568
           L+Y  RVSEAV+TPAF+NMQERMTHI  TE+++ RSLRQMGIDQLTRDPDL
Sbjct: 421 LFYCGRVSEAVSTPAFRNMQERMTHIRTTEQHSMRSLRQMGIDQLTRDPDL 471

BLAST of Spg018432 vs. ExPASy TrEMBL
Match: A0A6J1ILS8 (uncharacterized protein LOC111476761 OS=Cucurbita maxima OX=3661 GN=LOC111476761 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 8.2e-180
Identity = 349/470 (74.26%), Postives = 387/470 (82.34%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           M HS+I+R S KP+LDWEAALK+YENV+A+ESEAVKVKAT+KLA LS  AP NILNSTI 
Sbjct: 1   MSHSEIRRASEKPNLDWEAALKQYENVMATESEAVKVKATIKLASLSNDAPHNILNSTIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
           IIAKHLE N I NSS SM+G AAYCL+ IS QGDGTLATA+ HSGAL+S+LR L  + G 
Sbjct: 61  IIAKHLEVNPISNSSQSMRGAAAYCLKRISRQGDGTLATAIAHSGALESILRSLPDTSGG 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           FRKILVKC+WSIVT DK S VI+ARNGGLEV+IGMFDSV+DGT+RYLLEILSA+AL+REV
Sbjct: 121 FRKILVKCIWSIVTCDKHSRVIVARNGGLEVVIGMFDSVVDGTRRYLLEILSALALVREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKALISLRGLPFLVQAAR GCMASRERACQAIGL+AI++RGRHMLVELGV+ VLIELF E
Sbjct: 181 RKALISLRGLPFLVQAARYGCMASRERACQAIGLIAITRRGRHMLVELGVIPVLIELFCE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLVAGN+LGIVSAHVAYIRPVAQ G IPLFADLLQ PD   KEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVAGNSLGIVSAHVAYIRPVAQAGAIPLFADLLQWPDPIAKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           EANAV+I DHLVRILKEGDD  KAAAADVLWDLS YK SI +V SSGAIPVLVDLL  GN
Sbjct: 301 EANAVLIFDHLVRILKEGDDEGKAAAADVLWDLSSYKYSIPIVQSSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVE--------------------ILTGQGYPL 538
            EVREK S A+ QLSY+E DR ALADAGA+                     ++      L
Sbjct: 361 GEVREKVSGAVAQLSYSEADRIALADAGAIHGLIELLQDELDEVKDNAAEALINFSEDAL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           Y +RVSEA++ PAFQN+ ERMTHI ATER+  RSLRQMGI+QLT DPDLL
Sbjct: 421 YCNRVSEAISNPAFQNLLERMTHIRATERHGVRSLRQMGINQLTGDPDLL 470

BLAST of Spg018432 vs. ExPASy TrEMBL
Match: A0A5A7U359 (Vacuolar protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00230 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 1.5e-168
Identity = 336/470 (71.49%), Postives = 374/470 (79.57%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           MG ++I R S  P+LDWEA+LK+YENV+ASESEA+KVKAT+KLA LSK APENIL S I 
Sbjct: 1   MGDTKIHRASENPNLDWEASLKQYENVMASESEALKVKATIKLAQLSKNAPENILKSAIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
           IIAK LE    +N+S SM+  AAYCLRCISCQGDGTLA AV HSGAL+SLLR L HS GC
Sbjct: 61  IIAKPLEDKPTNNASQSMRAAAAYCLRCISCQGDGTLAKAVGHSGALESLLRSLLHSSGC 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           F KILVKC+WS VTFDK+S VI+ RNGGLEVII M   V DGT+RYLLEILSAMALLREV
Sbjct: 121 FCKILVKCIWSTVTFDKSSRVILPRNGGLEVIICMLVFVTDGTRRYLLEILSAMALLREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKAL+ LRGLPFLVQAAR G MASRERAC+AIGL+AI+KRGR+ LVELGVV+VLIEL RE
Sbjct: 181 RKALVGLRGLPFLVQAARFGYMASRERACEAIGLIAITKRGRNKLVELGVVTVLIELVRE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLVAGNALGIVSAH+AYIRPVAQ G IPLFADLLQ PD   KEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVAGNALGIVSAHLAYIRPVAQAGAIPLFADLLQSPDPICKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           E NAVVISDHLV++LKEGDD  KAAAADVL  LS YK SISVV +SGAIPVLVDLL  GN
Sbjct: 301 ETNAVVISDHLVKVLKEGDDRGKAAAADVLRILSSYKYSISVVQNSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVEILTG--------------------QGYPL 538
            EVREK S AI +LSY ETDR ALADAGA++ L G                       PL
Sbjct: 361 HEVREKVSGAIARLSYYETDRVALADAGAIQGLIGLLQDELEKVKLNVTEALTSFSKDPL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           YY R+SE V+TPAFQN+QER+THI ATE  +  S+ Q+GI+Q T D DL+
Sbjct: 421 YYTRLSEVVSTPAFQNLQERITHIRATEWQSMVSVHQLGINQFTFDLDLM 470

BLAST of Spg018432 vs. ExPASy TrEMBL
Match: A0A0A0LGL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G769710 PE=4 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 2.3e-166
Identity = 335/470 (71.28%), Postives = 373/470 (79.36%), Query Frame = 0

Query: 119 MGHSQIQRESAKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTIL 178
           MG  +I R S KP+LDWEA+LK+YENV+ASESEA+KVKAT+KLA LSK APENIL S I 
Sbjct: 1   MGDLKIHRASEKPNLDWEASLKQYENVMASESEALKVKATIKLAQLSKIAPENILKSVIP 60

Query: 179 IIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGC 238
           IIAK LE N  +N+S SM+  AAYCLRCISC+GDGTLATAV HSGAL+SL++ L HS GC
Sbjct: 61  IIAKPLEDNPTNNASQSMRAAAAYCLRCISCRGDGTLATAVGHSGALESLVKSLLHSSGC 120

Query: 239 FRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREV 298
           F KILVKC+WSIVTFD++S VIIARNGGLEVII M   VIDGT+RYLLEILSAMALLREV
Sbjct: 121 FCKILVKCIWSIVTFDQSSRVIIARNGGLEVIICMLVLVIDGTRRYLLEILSAMALLREV 180

Query: 299 RKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSVLIELFRE 358
           RKALI  RGLPFLVQAAR GCMASRERAC+AIGL+AI+KRGR+ LVELGVV VLIEL RE
Sbjct: 181 RKALIRSRGLPFLVQAARFGCMASRERACEAIGLIAITKRGRNKLVELGVVPVLIELLRE 240

Query: 359 GDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVA 418
           GD VTKLVAGN LGIVSAH+AYIRPVAQ G IPLFADLLQ  D   KEIAEDVFCLLAVA
Sbjct: 241 GDYVTKLVAGNTLGIVSAHLAYIRPVAQAGAIPLFADLLQWSDPICKEIAEDVFCLLAVA 300

Query: 419 EANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGN 478
           E NAVVI DHLV++LKEGDD  KAAAADVL  LS YK SISVV +SGAIPVLVDLL  GN
Sbjct: 301 ETNAVVIFDHLVKVLKEGDDRGKAAAADVLRILSSYKYSISVVQNSGAIPVLVDLLHDGN 360

Query: 479 DEVREKFSRAITQLSYNETDRAALADAGAVEILTG--------------------QGYPL 538
            EVREK S AI +LS  ETDR ALADAGA++ L G                       PL
Sbjct: 361 HEVREKVSGAIARLSCYETDRVALADAGAIQGLIGLLQDDLEKVKLNVTEAVTSFSKDPL 420

Query: 539 YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDLL 569
           Y  RV+EA++TPAFQN+QER+THI A E  +  S+ Q+GI+Q T D DLL
Sbjct: 421 YCTRVAEAISTPAFQNLQERITHIRAAEWQSMVSVHQLGINQFTFDLDLL 470

BLAST of Spg018432 vs. TAIR 10
Match: AT3G20170.1 (ARM repeat superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 1.0e-105
Identity = 219/476 (46.01%), Postives = 312/476 (65.55%), Query Frame = 0

Query: 119 MGHSQIQRES-AKPSLDWEAALKRYENVIASESEAVKVKATVKLAHLSKKAPENILNSTI 178
           MG S+ + ES    + DWE    R+EN I+S S +++V++ +KL+ L+ + PE+ ++  I
Sbjct: 1   MGSSETESESRGFQNPDWETEFNRFENAISSGSASIRVRSVLKLSDLTNRVPESYISRAI 60

Query: 179 LIIAKHLEGNLIDNSSHSMKGVAAYCLRCISCQG--DGTLATAVDHSGALQSLLRLLSHS 238
            I+A  L   + D+S+ S++  AA+CL+CI+C G  +   A  +   G + SLL LL  +
Sbjct: 61  PILAGLL--RVSDDSNRSVQAAAAHCLKCITCCGGEESGFAVTMGRCGVIASLLGLLLEA 120

Query: 239 R---GCFRKILVKCVWSIVTFDKTSCVIIARNGGLEVIIGMFDS-VIDGTKRYLLEILSA 298
                 FR+I VKC+WS+VTF  +  V +AR GGLE++I   ++   DG++ YLLEILSA
Sbjct: 121 NTDGNVFRRIWVKCLWSLVTFGSSIRVGLARLGGLEIVIRELNNWEDDGSRWYLLEILSA 180

Query: 299 MALLREVRKALISLRGLPFLVQAARCGCMASRERACQAIGLLAISKRGRHMLVELGVVSV 358
           +  +RE R+ L+   GL FLV+AA+ G +ASRERAC AIGL+ +++R R +LVE GV+  
Sbjct: 181 LTTIRESRRVLVHSGGLKFLVEAAKVGNLASRERACHAIGLIGVTRRARRILVEAGVIPA 240

Query: 359 LIELFREGDCVTKLVAGNALGIVSAHVAYIRPVAQGGVIPLFADLLQCPDLTGKEIAEDV 418
           L++L+R+GD   KL+AGNALGI+SA   YIRPV + G IPL+ +LL   D  GK+IAEDV
Sbjct: 241 LVDLYRDGDDKAKLLAGNALGIISAQTEYIRPVTEAGSIPLYVELLSGQDPMGKDIAEDV 300

Query: 419 FCLLAVAEANAVVISDHLVRILKEGDDGAKAAAADVLWDLSGYKSSISVVHSSGAIPVLV 478
           FC+LAVAE NAV+I++ LVRIL+ GD+ AK AA+DVLWDL+GY+ S+SV+  SGAIP+L+
Sbjct: 301 FCILAVAEGNAVLIAEQLVRILRAGDNEAKLAASDVLWDLAGYRHSVSVIRGSGAIPLLI 360

Query: 479 DLLLVGNDEVREKFSRAITQLSYNETDRAALADAGAVEILT----GQGYPL--------- 538
           +LL  G+ E RE+ S AI+QLSYNE DR A +D+G + IL      +   L         
Sbjct: 361 ELLRDGSLEFRERISGAISQLSYNENDREAFSDSGMIPILIEWLGDESEELRDNAAEALI 420

Query: 539 -------YYDRVSEAVNTPAFQNMQERMTHIHATERYTTRSLRQMGIDQLTRDPDL 568
                  +Y RV EA+  P FQ+MQ R+  I A+     RS+R++ I  L  D DL
Sbjct: 421 NFSEDQEHYARVREAIGHPVFQSMQSRLARIRASHELMVRSMRRVTIQHLAHDHDL 474

BLAST of Spg018432 vs. TAIR 10
Match: AT5G42340.1 (Plant U-Box 15 )

HSP 1 Score: 57.4 bits (137), Expect = 4.4e-08
Identity = 42/133 (31.58%), Postives = 64/133 (48.12%), Query Frame = 0

Query: 384 VAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEANAVVISD-----HLVRILKEGDD 443
           +A  G IPL   LL  PD   +E A      L++ E N  +IS+     +++ IL+ G+ 
Sbjct: 417 IANAGAIPLLVQLLSYPDSGIQENAVTTLLNLSIDEVNKKLISNEGAIPNIIEILENGNR 476

Query: 444 GAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETD 503
            A+  +A  L+ LS    +   +  S  IP LVDLL  G    ++    A+  LS N  +
Sbjct: 477 EARENSAAALFSLSMLDENKVTIGLSNGIPPLVDLLQHGTLRGKKDALTALFNLSLNSAN 536

Query: 504 RAALADAGAVEIL 512
           +    DAG V+ L
Sbjct: 537 KGRAIDAGIVQPL 549

BLAST of Spg018432 vs. TAIR 10
Match: AT2G45720.1 (ARM repeat superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.2e-07
Identity = 84/394 (21.32%), Postives = 157/394 (39.85%), Query Frame = 0

Query: 144 NVIASESEAVKVKATVKLAHLSKKAPENILNSTILIIAKHLEGNLIDNSSHSMKGVAAYC 203
           NV  SE +  K+K    L  LS K   ++ +  +L+    L G +    S S + +  + 
Sbjct: 92  NVCVSEKQEGKLKMQSDLDSLSAKIDLSLKDCGLLMKTGVL-GEVTKPLSSSTQDLETFS 151

Query: 204 LRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGCFRKILVKCVWSIVTFDKTSCVIIAR 263
           +R                    + L RL        RK L + V  ++  D+ + +    
Sbjct: 152 VR--------------------ELLARLQIGHLESKRKALEQLV-EVMKEDEKAVITALG 211

Query: 264 NGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREVRKALISLRGLPFLVQAARCGCMASR 323
              +  ++ +  +     +   + ++ ++A        LIS   LP L++    G + ++
Sbjct: 212 RTNVASLVQLLTATSPSVRENAVTVICSLAESGGCENWLISENALPSLIRLLESGSIVAK 271

Query: 324 ERACQAIGLLAISKRGRHMLVELGVVSVLIELFREGDCVTKLVAGNALGIVSAHVAYIRP 383
           E+A  ++  ++IS      +V  G V  LIE+ + GD V++  +   L  +SA     + 
Sbjct: 272 EKAVISLQRMSISSETSRSIVGHGGVGPLIEICKTGDSVSQSASACTLKNISAVPEVRQN 331

Query: 384 VAQGGVIPLFADLLQCPDLTG-KEIAEDVFCLLAVAEANAV----VISDH---------- 443
           +A+ G++ +  ++L C  L G KE A +  CL  +  +N      VIS++          
Sbjct: 332 LAEEGIVKVMINILNCGILLGSKEYAAE--CLQNLTSSNETLRRSVISENGIQTLLAYLD 391

Query: 444 ------------------------------LVRILKEGDDGAKAAAADVLWDLSGYKSSI 493
                                         LV +LK G  GA+ AAA  +  ++    + 
Sbjct: 392 GPLPQESGVAAIRNLVGSVSVETYFKIIPSLVHVLKSGSIGAQQAAASTICRIATSNETK 451

BLAST of Spg018432 vs. TAIR 10
Match: AT2G45720.2 (ARM repeat superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 2.2e-07
Identity = 84/394 (21.32%), Postives = 157/394 (39.85%), Query Frame = 0

Query: 144 NVIASESEAVKVKATVKLAHLSKKAPENILNSTILIIAKHLEGNLIDNSSHSMKGVAAYC 203
           NV  SE +  K+K    L  LS K   ++ +  +L+    L G +    S S + +  + 
Sbjct: 92  NVCVSEKQEGKLKMQSDLDSLSAKIDLSLKDCGLLMKTGVL-GEVTKPLSSSTQDLETFS 151

Query: 204 LRCISCQGDGTLATAVDHSGALQSLLRLLSHSRGCFRKILVKCVWSIVTFDKTSCVIIAR 263
           +R                    + L RL        RK L + V  ++  D+ + +    
Sbjct: 152 VR--------------------ELLARLQIGHLESKRKALEQLV-EVMKEDEKAVITALG 211

Query: 264 NGGLEVIIGMFDSVIDGTKRYLLEILSAMALLREVRKALISLRGLPFLVQAARCGCMASR 323
              +  ++ +  +     +   + ++ ++A        LIS   LP L++    G + ++
Sbjct: 212 RTNVASLVQLLTATSPSVRENAVTVICSLAESGGCENWLISENALPSLIRLLESGSIVAK 271

Query: 324 ERACQAIGLLAISKRGRHMLVELGVVSVLIELFREGDCVTKLVAGNALGIVSAHVAYIRP 383
           E+A  ++  ++IS      +V  G V  LIE+ + GD V++  +   L  +SA     + 
Sbjct: 272 EKAVISLQRMSISSETSRSIVGHGGVGPLIEICKTGDSVSQSASACTLKNISAVPEVRQN 331

Query: 384 VAQGGVIPLFADLLQCPDLTG-KEIAEDVFCLLAVAEANAV----VISDH---------- 443
           +A+ G++ +  ++L C  L G KE A +  CL  +  +N      VIS++          
Sbjct: 332 LAEEGIVKVMINILNCGILLGSKEYAAE--CLQNLTSSNETLRRSVISENGIQTLLAYLD 391

Query: 444 ------------------------------LVRILKEGDDGAKAAAADVLWDLSGYKSSI 493
                                         LV +LK G  GA+ AAA  +  ++    + 
Sbjct: 392 GPLPQESGVAAIRNLVGSVSVETYFKIIPSLVHVLKSGSIGAQQAAASTICRIATSNETK 451

BLAST of Spg018432 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 4.1e-06
Identity = 39/133 (29.32%), Postives = 63/133 (47.37%), Query Frame = 0

Query: 384 VAQGGVIPLFADLLQCPDLTGKEIAEDVFCLLAVAEANAVVIS-----DHLVRILKEGDD 443
           +A+ G IP+  +LL   D+  +E A      L++ E N  +I        +V++L+ G  
Sbjct: 369 IAEAGAIPVLVNLLTSEDVATQENAITCVLNLSIYENNKELIMFAGAVTSIVQVLRAGTM 428

Query: 444 GAKAAAADVLWDLSGYKSSISVVHSSGAIPVLVDLLLVGNDEVREKFSRAITQLSYNETD 503
            A+  AA  L+ LS    +  ++  SGAIP LVDLL  G    ++  + A+  L     +
Sbjct: 429 EARENAAATLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAATALFNLCIYHGN 488

Query: 504 RAALADAGAVEIL 512
           +     AG V  L
Sbjct: 489 KGRAVRAGIVTAL 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897634.11.3e-19279.15protein spotted leaf 11 [Benincasa hispida] >XP_038897635.1 protein spotted leaf... [more]
XP_022936629.11.5e-18075.11uncharacterized protein LOC111443172 [Cucurbita moschata][more]
XP_022139563.12.6e-18076.22uncharacterized protein LOC111010437 [Momordica charantia][more]
KAG6592081.13.4e-18074.89U-box domain-containing protein 12, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022976328.11.7e-17974.26uncharacterized protein LOC111476761 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q681N26.2e-0731.58U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2... [more]
A2ZLU62.0e-0531.11Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=... [more]
Q0IMG92.0e-0531.11E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SP... [more]
Q8GUG95.8e-0529.32U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Q9SNC65.8e-0529.85U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1F8U27.4e-18175.11uncharacterized protein LOC111443172 OS=Cucurbita moschata OX=3662 GN=LOC1114431... [more]
A0A6J1CFX31.3e-18076.22uncharacterized protein LOC111010437 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1ILS88.2e-18074.26uncharacterized protein LOC111476761 OS=Cucurbita maxima OX=3661 GN=LOC111476761... [more]
A0A5A7U3591.5e-16871.49Vacuolar protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G0... [more]
A0A0A0LGL62.3e-16671.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G769710 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G20170.11.0e-10546.01ARM repeat superfamily protein [more]
AT5G42340.14.4e-0831.58Plant U-Box 15 [more]
AT2G45720.12.2e-0721.32ARM repeat superfamily protein [more]
AT2G45720.22.2e-0721.32ARM repeat superfamily protein [more]
AT1G23030.14.1e-0629.32ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 336..376
e-value: 20.0
score: 10.8
coord: 454..494
e-value: 1.6
score: 17.8
coord: 377..417
e-value: 77.0
score: 6.4
coord: 295..335
e-value: 60.0
score: 7.2
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 465..507
score: 9.8174
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 128..283
e-value: 3.5E-8
score: 35.1
coord: 407..515
e-value: 2.4E-13
score: 52.0
coord: 284..406
e-value: 3.1E-14
score: 55.0
NoneNo IPR availablePANTHERPTHR46043:SF11ARM REPEAT SUPERFAMILY PROTEINcoord: 126..567
NoneNo IPR availablePANTHERPTHR46043ARM REPEAT SUPERFAMILY PROTEINcoord: 126..567
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 145..511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg018432.1Spg018432.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding