Spg018316 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg018316 vs. NCBI nr
Match: XP_030958760.1 (uncharacterized protein LOC115980671 [Quercus lobata])

HSP 1 Score: 215.3 bits (547), Expect = 2.4e-51
Identity = 136/413 (32.93%), Postives = 211/413 (51.09%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K   R  ++ K  + F  GL +P  G+SGGL L W+ EI+V ++S+S+ HIDAI+ ++
Sbjct: 36  ETKLRVRSMERKKMSIGFTNGLVIPSHGRSGGLALLWRKEINVDVQSFSDRHIDAIVTED 95

Query: 366 HGF-WKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRNP 425
            GF W+ +GFYGNPE  +R  SW L++ L     +PW+  GDFNEI+S  EK GGARR+ 
Sbjct: 96  RGFKWRITGFYGNPEVHRRKESWELLKVLSRKFQLPWLCFGDFNEIVSTSEKMGGARRSQ 155

Query: 426 SQMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKVT 485
            QM  FRE ID C+ MD G+ G +FTW   +  R ++   LDR L+    A    + +V 
Sbjct: 156 RQMDDFREAIDCCRFMDLGFCGPEFTWCNMQEGRHRMYLRLDRALVTQDWADHYKDVRVH 215

Query: 486 HLSYLSSDHKPIVATWDF-LDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQ-NDS 545
           HL    SDH  ++ T    L   +K R H     FE  W +  E +DI++ +W    N  
Sbjct: 216 HLVESVSDHCALLITDSIVLQSPRKRRFH-----FEAMWTRRDECRDIIRTAWNDSVNLY 275

Query: 546 CAQGLNTKVKNSLLNLHKWNRGRLKGSLKKAIERKEEEILRLTNEDND-LNFEEILEAEK 605
              G+   +K    +L +WN G++ G + + I+ K   +  L   D+D  N  EI +  K
Sbjct: 276 SPNGMVAGLKQCADDLSRWN-GKVFGRVPRQIQNKRRVLNELVLRDHDGSNGFEINKIRK 335

Query: 606 ELDNLLRLKE-----------------------------NEETTLKGFFSEHGVWEEDEG 665
           E+++LL  +E                              ++ T+     E G+W E   
Sbjct: 336 EINDLLDCEEIMWQQRSKVQWMGLGDRNTRYFHTKASERKKKNTISKILDERGMWRESAL 395

Query: 666 KIGAIATEYFQDLFQSSEPDGENVRKVVDCVSTKIS---DQQRVKELIKSNGE 683
           +I  +A  YF+ L+++S PD   + +VV+ +  K+S   +Q  +K+  +   E
Sbjct: 396 EIAEVAVSYFEKLYRTSNPD--KIAEVVEAIDPKVSAEMNQSLIKQFTREEVE 440

BLAST of Spg018316 vs. NCBI nr
Match: XP_022157437.1 (uncharacterized protein LOC111024135 [Momordica charantia])

HSP 1 Score: 214.5 bits (545), Expect = 4.1e-51
Identity = 120/285 (42.11%), Postives = 163/285 (57.19%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K +     + KR+L+F+  ++V   GKSGGLML W ++ +V I+S S GHID+II   
Sbjct: 36  ETKGNPTLEGRAKRELSFDCCVSVASTGKSGGLMLLWNSDSNVRIQSMSPGHIDSIITDK 95

Query: 366 HGFWKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRNPS 425
           +G W+F+GFYGNP T KR  SW L+E+L    D+PWIIGGDFNEI+S  EK GG  RN S
Sbjct: 96  YGSWRFTGFYGNPCTYKRSASWKLLERLARMMDLPWIIGGDFNEIVSMTEKMGGVCRNES 155

Query: 426 QMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKVTH 485
           QM+        C +           W R           LDR+LIN +M  + +N KVTH
Sbjct: 156 QMR-------GCPI-----------WER-----------LDRFLINESMLNKCLNLKVTH 215

Query: 486 LSYLSSDHKPIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQNDSCAQ 545
           L  LSSDH+PI+A+WDF        +  + +RFEE WL+    +DI+  +W        +
Sbjct: 216 LELLSSDHRPILASWDFELPRAWTCHKQRKIRFEESWLQIDGCRDIITGTWGSLPGIGIE 275

Query: 546 GLNTKVKNSLLNLHKWNRGRLKGSLKKAIERKEEEILRLTNEDND 591
               K+ + L  L++WN+ RL  SLK AI  KE+E+ RL   D D
Sbjct: 276 AFQAKICSCLSRLNEWNKIRLNRSLKGAIAYKEKELERLRQLDGD 291

BLAST of Spg018316 vs. NCBI nr
Match: XP_030970961.1 (uncharacterized protein LOC115991405 [Quercus lobata])

HSP 1 Score: 214.2 bits (544), Expect = 5.4e-51
Identity = 136/413 (32.93%), Postives = 211/413 (51.09%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K   R  ++ K  + F  GL +P  G+SGGL L W+ EI+V ++S+S+ HIDAI+ ++
Sbjct: 36  ETKLRVRSMERKKMSIGFTNGLVIPSHGRSGGLALLWRKEINVDVQSFSDRHIDAIVTED 95

Query: 366 HGF-WKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRNP 425
            GF W+ +GFYGNPE  +R  SW L++ L     +PW+  GDFNEI+S  EK GGARR+ 
Sbjct: 96  RGFKWRITGFYGNPEVHRRKESWELLKVLSRKFQLPWLCFGDFNEIVSTSEKMGGARRSQ 155

Query: 426 SQMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKVT 485
            QM  FRE ID C+ MD G+ G +FTW   +  R ++   LDR L+    A    + +V 
Sbjct: 156 RQMDDFREAIDCCRFMDLGFCGLEFTWCNMQEGRHRMYLRLDRALVTQDWADHYKDVRVH 215

Query: 486 HLSYLSSDHKPIVATWDF-LDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQ-NDS 545
           HL    SDH  ++ T    L   +K R H     FE  W +  E +DI++ +W    N  
Sbjct: 216 HLVESVSDHCALLITDSIVLQSPRKRRFH-----FEAMWTRRDECRDIIRTAWNDSVNLY 275

Query: 546 CAQGLNTKVKNSLLNLHKWNRGRLKGSLKKAIERKEEEILRLTNEDND-LNFEEILEAEK 605
              G+   +K    +L +WN G++ G + + I+ K   +  L   D+D  N  EI +  K
Sbjct: 276 SPNGMVAGLKQCADDLSRWN-GKVFGWVPRQIQNKRRVLNELVLRDHDGSNGFEINKIRK 335

Query: 606 ELDNLLRLKE-----------------------------NEETTLKGFFSEHGVWEEDEG 665
           E+++LL  +E                              ++ T+     E G+W E   
Sbjct: 336 EINDLLDCEEIMWQQRSKVQWMGLGDRNTRYFHTKASERKKKNTISKILDERGMWRESAL 395

Query: 666 KIGAIATEYFQDLFQSSEPDGENVRKVVDCVSTKIS---DQQRVKELIKSNGE 683
           +I  +A  YF+ L+++S PD   + +VV+ +  K+S   +Q  +K+  +   E
Sbjct: 396 EIAEVAVSYFEKLYRTSNPD--KIAEVVEAIDPKVSAEMNQSLIKQFTREEVE 440

BLAST of Spg018316 vs. NCBI nr
Match: XP_030939647.1 (uncharacterized protein LOC115964488 [Quercus lobata])

HSP 1 Score: 204.1 bits (518), Expect = 5.6e-48
Identity = 126/392 (32.14%), Postives = 205/392 (52.30%), Query Frame = 0

Query: 317 IKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKNHG--FWKFSGF 376
           IKR L  + GL VP   + GGL L WK+   V +++YS  HIDAI+ +  G   W+F+GF
Sbjct: 4   IKRKLERQQGLVVPSVRRGGGLALLWKSSTKVDVQTYSPRHIDAIVTEEQGKKKWRFTGF 63

Query: 377 YGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRNPSQMKLFRETI 436
           YGN ET+KR  SW L+E+L ++ D+PW+  GDFNEI+   EK+GG+ R   QM++F ET+
Sbjct: 64  YGNSETNKREESWKLLEQLSKSCDLPWVCMGDFNEIMHSSEKEGGSARPEGQMRIFCETV 123

Query: 437 DRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKVTHLSYLSSDHK 496
           ++C L D GYSG  FTW R    R  + E LDR  ++          ++ H++  +SDH 
Sbjct: 124 NKCNLRDMGYSGPDFTWSRRLGSRGWVRERLDRAFVSTNWTSSFPRAQLFHVTTSASDHC 183

Query: 497 PIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQNDSCAQGLNTK-VKN 556
            +V   D  +  ++     KL RFE  WL+     +IV  +W +     ++   +K ++ 
Sbjct: 184 MLVLKAD--NTRRRNLRRPKLFRFEAMWLRDDRCDEIVTTAWERGVYGGSEWPFSKCMEE 243

Query: 557 SLLNLHKWNR------GRLKGSLKKAIERKEEEILRLTNEDNDLNFEEILEAEKELDNLL 616
              +L  WN+      GR   SL++ I+  EE+        N+   E+I + + EL+ ++
Sbjct: 244 CRTSLVSWNKNTFGNVGRKISSLQRKIQYLEEK------GGNNAAMEDIHKTKMELNKMI 303

Query: 617 RLKE-----------------------------NEETTLKGFFSEHGVWEEDEGKIGAIA 671
           R +E                             N+  T+      +GVW++++ +IG++ 
Sbjct: 304 RAEEDMWHQRSRNCWLKSGDSNTSFFHTKASNRNQRNTISKIMDSNGVWQDEDVRIGSVF 363

BLAST of Spg018316 vs. NCBI nr
Match: XP_030929385.1 (uncharacterized protein LOC115955407 [Quercus lobata])

HSP 1 Score: 203.0 bits (515), Expect = 1.2e-47
Identity = 128/412 (31.07%), Postives = 207/412 (50.24%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K   +  ++ K  + F  GL +P  G+SGGL L W+ EI+V ++S+S+ HIDAI+ ++
Sbjct: 36  ETKLRMQSMERKKMSIGFTNGLVIPSHGRSGGLALLWRKEINVDVQSFSDRHIDAIVTED 95

Query: 366 HGF-WKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRNP 425
            GF W+ +GFYGNP+  +R  SW L++ L     +PW+  G+FNEI+S  EK GGARR+ 
Sbjct: 96  RGFKWRITGFYGNPKVHRRKESWELLKVLSRKFQLPWLCFGEFNEIVSTSEKMGGARRSQ 155

Query: 426 SQMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKVT 485
            QM  FRE I+ C+ MD G+ G +FTW   +  R ++   LDR L+    A    + +V 
Sbjct: 156 RQMDDFREAINYCRFMDLGFYGPEFTWYNMQEGRHRMYLRLDRALVTQDWADHYKDVRVH 215

Query: 486 HLSYLSSDHKPIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQ-NDSC 545
           HL    SDH  ++ T   +  +   R      +FE  W +  E +DI++  W    N   
Sbjct: 216 HLVESMSDHCALLITDSIVLQSPWKRR----FQFEAMWTRRDECRDIIRTVWNDSVNLYS 275

Query: 546 AQGLNTKVKNSLLNLHKWNRGRLKGSLKKAIERKEEEILRLTNEDND-LNFEEILEAEKE 605
             G+   +K    +L +WN G + G + + I+ K   +  L    +D  N  EI +  KE
Sbjct: 276 PNGMVAGLKQCADDLSRWN-GEVFGRVPRQIQNKRRVLNELVLRVHDGSNGYEINKIRKE 335

Query: 606 LDNLLRLKE-----------------------------NEETTLKGFFSEHGVWEEDEGK 665
           +++LL  +E                              ++ T+     E G+W E   +
Sbjct: 336 INDLLECEEIMWQQRSKVQWMGLGDRNTRYFHTKASERKKKNTISKILDERGMWRESALE 395

Query: 666 IGAIATEYFQDLFQSSEPDGENVRKVVDCVSTKIS---DQQRVKELIKSNGE 683
           I  +A  YF+ L+++S PD   + +VV+ +  K+S   +Q  +K+  +   E
Sbjct: 396 IAEVAVSYFEKLYRTSNPD--KIGEVVEAIDPKVSAEMNQSLIKQFTREEVE 440

BLAST of Spg018316 vs. ExPASy TrEMBL
Match: A0A6J1DUG8 (uncharacterized protein LOC111024135 OS=Momordica charantia OX=3673 GN=LOC111024135 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.0e-51
Identity = 120/285 (42.11%), Postives = 163/285 (57.19%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K +     + KR+L+F+  ++V   GKSGGLML W ++ +V I+S S GHID+II   
Sbjct: 36  ETKGNPTLEGRAKRELSFDCCVSVASTGKSGGLMLLWNSDSNVRIQSMSPGHIDSIITDK 95

Query: 366 HGFWKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRNPS 425
           +G W+F+GFYGNP T KR  SW L+E+L    D+PWIIGGDFNEI+S  EK GG  RN S
Sbjct: 96  YGSWRFTGFYGNPCTYKRSASWKLLERLARMMDLPWIIGGDFNEIVSMTEKMGGVCRNES 155

Query: 426 QMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKVTH 485
           QM+        C +           W R           LDR+LIN +M  + +N KVTH
Sbjct: 156 QMR-------GCPI-----------WER-----------LDRFLINESMLNKCLNLKVTH 215

Query: 486 LSYLSSDHKPIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQNDSCAQ 545
           L  LSSDH+PI+A+WDF        +  + +RFEE WL+    +DI+  +W        +
Sbjct: 216 LELLSSDHRPILASWDFELPRAWTCHKQRKIRFEESWLQIDGCRDIITGTWGSLPGIGIE 275

Query: 546 GLNTKVKNSLLNLHKWNRGRLKGSLKKAIERKEEEILRLTNEDND 591
               K+ + L  L++WN+ RL  SLK AI  KE+E+ RL   D D
Sbjct: 276 AFQAKICSCLSRLNEWNKIRLNRSLKGAIAYKEKELERLRQLDGD 291

BLAST of Spg018316 vs. ExPASy TrEMBL
Match: A0A2N9IPS8 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55418 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 8.7e-47
Identity = 127/401 (31.67%), Postives = 201/401 (50.12%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAII--- 365
           E + D    ++++  + F+    VP +G  GGL + W A++DV + +YS  HIDA I   
Sbjct: 476 ETRLDKIGVERLRVTIKFDGAFCVPRRGTGGGLAVLWIAKLDVKLTTYSRNHIDAEIVDK 535

Query: 366 KKNHGFWKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARR 425
           +K  GF + +GFYGNPET KR  SW L++ L      PW+  GDFNEI+   E+ G   R
Sbjct: 536 EKGKGF-RLTGFYGNPETHKRKESWALLKHLSHLSSSPWLCMGDFNEILDNNERLGSGHR 595

Query: 426 NPSQMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFK 485
              Q++ FRE +  C L D GY GN +TWRR ++    +   LDR + + +         
Sbjct: 596 PEWQIRDFREAVVHCGLHDLGYVGNSYTWRRKRDGNALVTARLDRMMASVSWLTDYDGSV 655

Query: 486 VTHLSYLSSDHKPIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQNDS 545
           V+HL+  +SDH PI+     + D   V+   KL RFE  W+K ++ ++++  +W    D 
Sbjct: 656 VSHLAVQNSDHCPILLD---IPDGPLVKRKKKLFRFEAMWIKDEQCREVIDHAW---GDG 715

Query: 546 CAQG-----LNTKVKNSLLNLHKWNRGRLKGSLKKAIERKEEEILRLTNEDNDLNFEEIL 605
             +G     +  K+K    +L  W+R R  GSL  +I+RK E++  L NE        IL
Sbjct: 716 VTEGSPMFMVVEKMKLCRTSLIGWSRERF-GSLASSIKRKREQLQHLINETPSGFSTGIL 775

Query: 606 EAEKELDNLLR--------------LKENEETT---------------LKGFFSEHGVWE 665
           E + +L+ LL               + E ++ T               + G     GVW+
Sbjct: 776 ELQDDLNGLLEKEEIFWRQRSRVAWMSEGDKNTKFFHAQCNERRRTNHISGLRDRDGVWQ 835

Query: 666 EDEGKIGAIATEYFQDLFQSSEPDGENVRKVVDCVSTKISD 670
            ++ KI  IA +YFQ +F SS P  E++  V+  + + +++
Sbjct: 836 TEKTKIAEIAVDYFQGIFTSSNPSAESITTVLQGMESVVTN 868

BLAST of Spg018316 vs. ExPASy TrEMBL
Match: A0A5B6V1B3 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_029333 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 9.6e-46
Identity = 124/382 (32.46%), Postives = 199/382 (52.09%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K D+++ D++++   F  G++V  +G  GGL L WK  I+V +R+YS+ HID +IK++
Sbjct: 2   ETKLDSKRMDRVRKSCGFMNGIDVGAEGSRGGLCLAWKGGIEVMLRTYSKWHIDVLIKED 61

Query: 366 --HGFWKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRN 425
                W+F+GFYG+P    +   WNL+E+L ++ D PW + GDFNEI+   EKK G  R+
Sbjct: 62  GIQEEWRFTGFYGSPYVRDQNHVWNLLERLSQDRDYPWFVAGDFNEIMYSFEKKEGLPRD 121

Query: 426 PSQMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKV 485
           P +M+ FR+T+  C LMD GYSG  FTW RG      I E LDR + N          ++
Sbjct: 122 PRRMETFRDTLANCGLMDVGYSGTWFTWERGNLPETNIRERLDRGVANEKWLSLFPMGRI 181

Query: 486 THLSYLSSDHKPIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQNDSC 545
            HL +++S H P++ T D  +  ++ R       FE  W   +    ++++ W    +S 
Sbjct: 182 QHLPFVASYHCPLLLTTDSDNPFKRKRR----FHFETWWTMEESFAGVLQNIW----ESS 241

Query: 546 AQGLNTKVKNSLLNLHKWNRG--RLKGSLKKAIERKEEEILRLTNEDN------DLNFEE 605
           ++ L  K+K   + L KW     R KG +KK +    E +L    +D       D     
Sbjct: 242 SEPLMQKLKTLQVGLEKWANALKRKKGEIKKKLTEDIEALLMEDRDDETLAKLIDTKIHL 301

Query: 606 ILEAEKELDNLLRLKENEETTLKGFF-----SEHGVWEEDEGKIGAIATEYFQDLFQS-S 665
            LE +K+  N     ++  T  K  F     S++G    DE  +   A  YF++LF +  
Sbjct: 302 NLEIDKDERNTAFFHKSATTRQKANFITKLVSDNGSEIIDEIGLQEAAKSYFENLFATEG 361

Query: 666 EPDGENVRKVVD-CVSTKISDQ 671
             D + V + +D C++ +I+D+
Sbjct: 362 VVDPKKVLEGIDRCITQEINDE 375

BLAST of Spg018316 vs. ExPASy TrEMBL
Match: A0A5B6U6G4 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_009510 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.3e-45
Identity = 128/397 (32.24%), Postives = 199/397 (50.13%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K D++  D+++R   F  G+NV  +G  GGL L W+ +I V +RSYS+ HID +IK+ 
Sbjct: 36  ETKLDSKCMDRVRRSCGFTNGINVEAEGSRGGLCLAWRDDIGVTLRSYSKWHIDVLIKEE 95

Query: 366 --HGFWKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRN 425
                W+F+GFYG+P    + + WNL+E+L ++ D PW++ GDFNEI+   EK+GG  R+
Sbjct: 96  GIQEEWRFTGFYGSPYVRDQKYVWNLLERLSQDKDYPWLVAGDFNEIMFSFEKQGGQLRD 155

Query: 426 PSQMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKV 485
           P +M+ FR+T+  C LMD G+SG  FTW RG      I E LDR + N          ++
Sbjct: 156 PRRMETFRDTLANCGLMDVGFSGTWFTWERGNLSETNIRERLDRGVANEKWLTLFPMGRI 215

Query: 486 THLSYLSSDHKPIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQNDSC 545
            HL ++ SDH P++ T D   D   +RN  +   FE  W   +  + ++++ W    +S 
Sbjct: 216 QHLPFIISDHCPLLLTTD--SDNTIIRN--RRFHFETWWTMEESFEGVLRNIW----ESS 275

Query: 546 AQGLNTKVKNSLLNLHKWNR--GRLKGSLKKAIERKEEEILRLTNEDN------DLNFEE 605
           ++ L  K+K   + L +W +   R KG LKK +    E +L    +D       D     
Sbjct: 276 SEPLIQKLKTLQVGLEQWAKTLKRKKGVLKKKLTEDLESLLGKDRDDETLAKLIDTKIRL 335

Query: 606 ILEAEKEL---------------DNLLRLKENEETTLK------GFFSEHGVWEEDEGKI 665
            LE +KE                D          TT K          + G+   DE  +
Sbjct: 336 NLEIDKEERYWEQRSRVNWLQFGDRNTAFFHKSATTRKKVNLITKLVLDEGLEITDETGL 395

Query: 666 GAIATEYFQDLFQS-SEPDGENVRKVVD-CVSTKISD 670
              A  YF++LF S    D + V + ++ C+S +++D
Sbjct: 396 QEAAKSYFENLFTSEGVADPKKVLEGIESCISQEVND 424

BLAST of Spg018316 vs. ExPASy TrEMBL
Match: A0A5B6UW38 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_012566 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 8.2e-45
Identity = 119/380 (31.32%), Postives = 197/380 (51.84%), Query Frame = 0

Query: 306 EEKCDARKSDKIKRDLNFEFGLNVPCKGKSGGLMLCWKAEIDVHIRSYSEGHIDAIIKKN 365
           E K D++++D++++   F  G++V  +G  GGL L WK  I+V +RSYS+ HID +IK++
Sbjct: 2   ETKLDSKRNDRVRKSCGFINGIDVEAEGSRGGLCLAWKGGIEVTLRSYSKWHIDVLIKED 61

Query: 366 --HGFWKFSGFYGNPETDKRIFSWNLMEKLCENDDIPWIIGGDFNEIISEEEKKGGARRN 425
                W+F+GFYG+P    R   WNL+ +L ++ D PW + GDFN+I+   EKKGG  R+
Sbjct: 62  SIQEEWRFTGFYGSPYDRDRNHVWNLLGRLSQDRDYPWFVAGDFNKIMYSFEKKGGLPRD 121

Query: 426 PSQMKLFRETIDRCKLMDGGYSGNKFTWRRGKNKRKQICESLDRYLINHTMAVQTVNFKV 485
           P +M+ FR+T+  C LMD GYSG  FTW RG      I E LD  + N          ++
Sbjct: 122 PRRMETFRDTLAYCGLMDVGYSGTWFTWERGNLPETNIRERLDCGVANEKWLSLFPMGRI 181

Query: 486 THLSYLSSDHKPIVATWDFLDDAQKVRNHGKLLRFEEGWLKFKETKDIVKDSWRQQNDSC 545
            HL +++SDH P++ T     D+       +   FE  W   +    +++  W    +S 
Sbjct: 182 QHLPFVASDHCPLLLT----TDSDNPYKSSRRFHFESWWTMEESFAGVLQSIW----ESS 241

Query: 546 AQGLNTKVKNSLLNLHKWNRGRLKGSLKKAIERKEEEILRLTNEDNDLNFEEILEAEKEL 605
           ++ L  K+K   + L KW          KA++RK+ EI +   ED    FE +L  +++ 
Sbjct: 242 SEPLMQKLKTLQVGLEKW---------AKALKRKKREIKKKLTED----FEALLMEDRDD 301

Query: 606 DNLLRLKENEETTLKGFFSEHGVWEEDEG----KIGAIATEYFQDLFQSSEPDGENVRKV 665
           + L +L + +         E   WE+       + G   T++F     ++      + K+
Sbjct: 302 ETLAKLIDTKIHLNWEIDKEERYWEQRARVNWLQFGDRNTDFFHK-SATTRKKANFISKL 359

Query: 666 VDCVSTKISDQQRVKELIKS 680
           V    ++I+D+  ++E +KS
Sbjct: 362 VSEDGSEITDEIGLQEAMKS 359

BLAST of Spg018316 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 9.9e-19
Identity = 60/207 (28.99%), Postives = 105/207 (50.72%), Query Frame = 0

Query: 767 KEEAERVAIILWSVWNCRNKLAINNEKPDLQQILRAINRNLEEQRKRQDTNTALMSLE-N 826
           ++ ++ V  +LW +W  RN+L     + + Q++LR    +LEE R R +  +     + N
Sbjct: 354 EKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVN 413

Query: 827 LRNQGTWNPPPPPNCVKLNTGASWNEERKRGGVGWIFRDSSGSPIGFGCKQINRPWSIKS 886
             + G W  PPP   VK NT A+WN + +R G+GW+ R+  G     G + + +  S+  
Sbjct: 414 RSSCGRWR-PPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLE 473

Query: 887 LELLAIKEGLNAYREEGQFRSAPIIVEADALEVIRVLNHETLDLSDSKLVVNDIEILASS 946
            EL A++    A     +F+   +I E+D+  +I +LN++ +     K  + D++ L S 
Sbjct: 474 AELEAMRW---AVLSLSRFQYNYVIFESDSQVLIEILNNDEI-WPSLKPTIQDLQRLLSQ 533

Query: 947 CNVISFVKCLRAGNKIAHHLARSAAGF 973
              + FV   R GN +A  +AR +  F
Sbjct: 534 FTEVKFVFIPREGNTLAERVARESLSF 555

BLAST of Spg018316 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.2e-16
Identity = 64/222 (28.83%), Postives = 102/222 (45.95%), Query Frame = 0

Query: 776 ILWSVWNCRNKLAINNEKPDLQQILRAINRNLEEQRKRQDTNTALMSLENLRNQGTWNPP 835
           +LW +W  RN+L    ++ D  ++LR    + EE   R++        +  RN       
Sbjct: 80  LLWRLWKSRNELMFKGKEYDAPEVLRRAMEDFEEWSTRRELEGKASGPQVERNLSVQWKA 139

Query: 836 PPPNCVKLNTGASWNEERKRGGVGWIFRDSSGSPIGFGCKQINRPWSIKSLELLAIKEGL 895
           PP   VK NT A+W  E  R G+GWI R+ SG  +  G + + R  ++   EL A++   
Sbjct: 140 PPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRW-- 199

Query: 896 NAYREEGQFRSAPIIVEADALEVIRVLNHETLDLSDSKLVVNDIEILASSCNVISFVKCL 955
            A     +F    II E+DA  ++ +LN +       +  + DI+ L      + F    
Sbjct: 200 -AVLTMSRFNYKRIIFESDAQALVNLLNSDDF-WPTLQPALEDIQQLLHHFEEVKFEFTP 259

Query: 956 RAGNKIAHHLARSAAGFAS--AGLLSVVAVDGAYEFWVSSTL 996
           R GNK+A  +AR +  F++    L S+V        W+ STL
Sbjct: 260 RGGNKVADRIARESISFSNYDPKLFSIVPQ------WLRSTL 291