Spg018049 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg018049
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	subtilisin-like protease SBT3.11
Location	scaffold9: 27173810 .. 27176413 (-)
RNA-Seq Expression	Spg018049
Synteny	Spg018049

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAAACAAAATAATTTTAATTAAAAAAAAAACAAAATAAATAATAAATAAATAAAAAAGGCAAAAGGCAAAAGTGTGTGTCGCGACTTTGAAGAGGCATCGGGTCCCAAATTCTAACCTTGTTCTCTTCTCTTGATACTCGCTCTAATCTCTATCACTATATCATGCACAAGTTGACATGGCGAAGTTGAATTCTTCTTCGTTTCCCCTATATCTCCTTTTGCTCTTCTCAATTTCTCTCAATTTGCTGCTTAGTTCTTCCGCTATGTCCGGCCAATCCGCCGACTCCAAGTCCTCTTCTCCGGCGGTGCATATCGTTTACACGGAGAGGCCTCGCGATGAGGAACCTGAGGCCTACCACATCCGAACCCTAACTTCTGTTCTTGGCAGGTCTGTTTCCTGATTACTCCCTTGTGTGTGTCTTTTGGCTGCAAAACGGTGGAAATTGACCGTCGATTTTGCTTTCATTTGTTGATTTGCAGTGAAGAGGCTGCTAGAGAGGCTCTTGTCTATAGTTACAAGAATGCTGCCAGTGGCTTCTCTGCTCGGCTTACTCCCGATCAGGTCGCGGAGATTTCCAGTAAGTTGTTTGCGATCTTCCTCGTTGATTTATTTGTCATTGATTGATTAATTTGTTCTGTGAAATTTTATGCTATTTCGTCTATTTAGTGGAGTTCTGGAAGGTGTTAATGCATTTTGAGCATAGCAGGTAAAAATTTAGGGAAATTCATTTCGGTTTGGCTTATTTGATTTTATCACGTTGCACCCAAATCCAAACTTGAATTATTTTTGAAGAGTTTTTGGTCAGCCATGAGTATGATTGTGGTCGGAAGAGACTTGTTTTATGTTTTGTACTAGTCATTTCCGTTCTGTTGGATACGCAGTGACTGAATTTGGATATTTTATTGTTGAATTTAAGTACTATTTTATATGATCAATATCTTGTAGCTAGGTTTTAGTAGATGAGATATCCCACCATGCCAAGGGGATTACAGAAAGAACTGTCAATTAGCAAAGATTTGTAAGAAGCTATAATTACATAATCTTAGCATAGGCACACCAAGATCCCAAAAACACATAACGGCACACATCCTCTCCTGAAAAGATTCTCTTGTTTCTCTTGCAAGTCATACCAGTCTCCACAAAAGAGCTACCAACCCCATTGATCCATAGATACTTCGCTTGTCCTCAGAAATCAGTACCATTAAAAGAGCTGGAAAAGGGCCCCTGGCATTAAAAAAAAAAAAAAAAAAAGAACCCAGCTGATGTTGAAAATTTGAAAAAATCAAACTAAGGTGCAGCTGGTTGTCCTCACTGTATTTGCACATCATTCATCAATAGGGTTTAGGATTGGGCATGTATGTTGCTAGGTTTCAGTATTTTCAAGGCTGGATTTGGGCATTTCTTAGGTTTGATTTCAAGTGTTTTGTTGGTTGAATTTAGTGTAATTTATTAGTTAGTTTTTAGGCAAATTTCTAATTTAGTTTTGTATAAGTATTAGTATCATCCTTATTCAAGTTTAGAGTTCAATTTGATGAAATTTGAAAATTTAAAGTTAATTGACTAACCAAACCAAGTACGTTGATAGAAAAAATTCTTTTACTTTCCTAATTTCGACGATGTATAGTTTCCAAATGTCTGGAGGTGCTGGGTTCTTTGGTTCGGTACCTGAAATGTGAAAAGCAGAGTGTCGAACAAAGCTTCTTATACTTATTTACTATGTCCTTGAAGTTGTTTATTGACTATCCAATAGTGAGTTGGATCAGGATTTTGATTGTCTCTTTACGCTGTCATATGTTTGCAGTCCAGCTTGATGCTTATAACATGTCACATGCTTATTTATATATAAGATTAAATATATCATATATAACCTATCAACTTAAACTTTAGAAATTAGTCTTACTTTAACATGGGACCGAAACAAGAGGTCCGAGGTTGAGCCTTAGAGCATCTTCGTGCATCAAATTAATATTTAAGCCCACACACTAAAAAGGGAGTGTTAATGAATATATATCAAAGATTACATATATCATAATCTATCAATTTAGGGGTGGTTTGACATATATTTGCATATGCTTTTCATATCTCTATAACTTGTATATCCCAATAACTCAGTATATCTCAAGAACATTAACATTAGTTCTTGTGTATATATATTTAGATATTCAGTTTAGAATGTGAATAAATCCCCACAATTACTACAAGCTACTGTTTCTTATGAACTTTGATGTTGGAACTTGGCAGAACAAGAAGGAGTTTTACAGGTTGTCCCAAGCCGTACTCTTCAGCTGCATTCAGGACCTGCAGGCCTTCACTAGAAGAACATCAAACTCTTTATCTGGAAATATGGCTCTTTCCAACGGTTTGAAAGTGCATACCTAAATAAAGTGTACTTTGTCTATATATATTGTGCTTGCTGAATGATCATATATATAATTGCTATACTATTATACTCGTGTATCTATTTTCTATACCATAATATGTGCGTTATGTTTTACCATTGATAAAGAGGCCAATGAGTTGTTGGTAAGGGTTTTTAAATGATAAGGGGAAGCAAAACCAAAATGTACCCAATTGGCAGCAAAAAAAAAATCTCCTCAGTCC

mRNA sequence

ATGGCGAAGTTGAATTCTTCTTCGTTTCCCCTATATCTCCTTTTGCTCTTCTCAATTTCTCTCAATTTGCTGCTTAGTTCTTCCGCTATGTCCGGCCAATCCGCCGACTCCAAGTCCTCTTCTCCGGCGGTGCATATCGTTTACACGGAGAGGCCTCGCGATGAGGAACCTGAGGCCTACCACATCCGAACCCTAACTTCTGTTCTTGGCAGTGAAGAGGCTGCTAGAGAGGCTCTTGTCTATAGTTACAAGAATGCTGCCAGTGGCTTCTCTGCTCGGCTTACTCCCGATCAGGTCGCGGAGATTTCCATGGAGTTCTGGAAGGTGTTAATGCATTTTGAGCATAGCAGTTTCCAAATGTCTGGAGGTGCTGGGTTCTTTGGTTCGAACAAGAAGGAGTTTTACAGGTTGTCCCAAGCCGTACTCTTCAGCTGCATTCAGGACCTGCAGGCCTTCACTAGAAGAACATCAAACTCTTTATCTGGAAATATGGCTCTTTCCAACGGTTTGAAAGTGCATACCTAA

Coding sequence (CDS)

ATGGCGAAGTTGAATTCTTCTTCGTTTCCCCTATATCTCCTTTTGCTCTTCTCAATTTCTCTCAATTTGCTGCTTAGTTCTTCCGCTATGTCCGGCCAATCCGCCGACTCCAAGTCCTCTTCTCCGGCGGTGCATATCGTTTACACGGAGAGGCCTCGCGATGAGGAACCTGAGGCCTACCACATCCGAACCCTAACTTCTGTTCTTGGCAGTGAAGAGGCTGCTAGAGAGGCTCTTGTCTATAGTTACAAGAATGCTGCCAGTGGCTTCTCTGCTCGGCTTACTCCCGATCAGGTCGCGGAGATTTCCATGGAGTTCTGGAAGGTGTTAATGCATTTTGAGCATAGCAGTTTCCAAATGTCTGGAGGTGCTGGGTTCTTTGGTTCGAACAAGAAGGAGTTTTACAGGTTGTCCCAAGCCGTACTCTTCAGCTGCATTCAGGACCTGCAGGCCTTCACTAGAAGAACATCAAACTCTTTATCTGGAAATATGGCTCTTTCCAACGGTTTGAAAGTGCATACCTAA

Protein sequence

MAKLNSSSFPLYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAYHIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQMSGGAGFFGSNKKEFYRLSQAVLFSCIQDLQAFTRRTSNSLSGNMALSNGLKVHT

Homology

[Close]

BLAST of Spg018049 vs. NCBI nr
Match: XP_022935866.1 (subtilisin-like protease SBT3.11 [Cucurbita moschata] >XP_023537186.1 subtilisin-like protease SBT3.11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 160.2 bits (404), Expect = 1.6e-35
Identity = 94/126 (74.60%), Postives = 105/126 (83.33%), Query Frame = 0


Query: 1   MAKLNSSSFPLYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAY 60
           MAKL  SSFPL  +LLFSISLNL+LSSSAMS QS DS S+SPAVHIVY E+PRDEEPEAY
Sbjct: 1   MAKLIFSSFPLCFVLLFSISLNLILSSSAMSAQSVDSASASPAVHIVYMEKPRDEEPEAY 60

Query: 61  HIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQM 120
           HIRTL SVLGSEEAAREALVYSYKNAASGFSA+LTP+QVAE+S +   VL      ++Q+
Sbjct: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQ-EGVLQVVPSRTYQL 120

Query: 121 -SGGAG 126
            SG AG
Sbjct: 121 HSGPAG 125

[Close]

BLAST of Spg018049 vs. NCBI nr
Match: XP_038897393.1 (subtilisin-like protease SBT3.17 [Benincasa hispida])

HSP 1 Score: 154.5 bits (389), Expect = 8.7e-34
Identity = 86/105 (81.90%), Postives = 94/105 (89.52%), Query Frame = 0


Query: 1   MAKLNSSSFPLYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAY 60
           MA LN SS    L+LLFS+SLNL+L+SSAMS QS DS S+SPAVHIVY E+PRDEEPEAY
Sbjct: 1   MANLNFSS----LVLLFSLSLNLVLTSSAMSAQSTDSNSASPAVHIVYMEKPRDEEPEAY 60

Query: 61  HIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISME 106
           HIRTLTSVLGSEEAAREAL+YSYKNAASGFSARLTPDQVAEIS +
Sbjct: 61  HIRTLTSVLGSEEAAREALLYSYKNAASGFSARLTPDQVAEISKQ 101

[Close]

BLAST of Spg018049 vs. NCBI nr
Match: XP_022143620.1 (subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia])

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 90/125 (72.00%), Postives = 98/125 (78.40%), Query Frame = 0


Query: 1   MAKLNSSSFPLYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAY 60
           MAK   SSF L L LLFSISLN LLSS+AMSGQ A     SPAVHIVYTE+PRDEEPEAY
Sbjct: 1   MAKTTFSSFLLCLALLFSISLNFLLSSAAMSGQYA-----SPAVHIVYTEKPRDEEPEAY 60

Query: 61  HIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQM 120
           HIRTL SVLGSEEAA+EALVYSYKNAASGFSARLTPDQVAE+S +   VL   +  + Q+
Sbjct: 61  HIRTLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQ-PGVLHVVQSGTHQL 119

Query: 121 SGGAG 126
             G G
Sbjct: 121 HSGGG 119

[Close]

BLAST of Spg018049 vs. NCBI nr
Match: KAG7025065.1 (Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 142.9 bits (359), Expect = 2.6e-30
Identity = 81/104 (77.88%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 6   SSSFPLYLLLLFSISLNLLLSSSAMSGQSAD----SKSSSPAVHIVYTERPRDEEPEAYH 65
           SSSFP  L+LLFSISLNL LSS+ MS QSA     S  +SPAVHIVYTERP +EEPEAYH
Sbjct: 7   SSSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYH 66

Query: 66  IRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISME 106
           IRTL SVLGSEEAAREALVYSYKNAASGFSARLTP+QVA+I+ +
Sbjct: 67  IRTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQ 110

[Close]

BLAST of Spg018049 vs. NCBI nr
Match: XP_023534872.1 (subtilisin-like protease SBT3.6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.9 bits (359), Expect = 2.6e-30
Identity = 81/104 (77.88%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 6   SSSFPLYLLLLFSISLNLLLSSSAMSGQSAD----SKSSSPAVHIVYTERPRDEEPEAYH 65
           SSSFP  L+LLFSISLNL LSS+ MS QSA     S  +SPAVHIVYTERP +EEPEAYH
Sbjct: 12  SSSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYH 71

Query: 66  IRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISME 106
           IRTL SVLGSEEAAREALVYSYKNAASGFSARLTP+QVA+I+ +
Sbjct: 72  IRTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQ 115

[Close]

BLAST of Spg018049 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 7.2e-07
Identity = 29/63 (46.03%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 44  VHIVYTERPRDEEPE---AYHIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVA 103
           VHIVY       +PE   + H+R L S+LGS++ A E++V+SY+N  SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

[Close]

BLAST of Spg018049 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.0e-05
Identity = 27/68 (39.71%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 39  SSSPAVHIVYTERPRDEEPE---AYHIRTLTSVLGSEEAAREALVYSYKNAASGFSARLT 98
           S+   VHIVY    + ++P+     H + L+S+LGS++ A E++VYSY++  SGF+A+LT
Sbjct: 24  SAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLT 83

Query: 99  PDQVAEIS 104
             Q  +I+
Sbjct: 84  KSQAKKIA 91

[Close]

BLAST of Spg018049 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.4e-05
Identity = 37/96 (38.54%), Postives = 56/96 (58.33%), Query Frame = 0


Query: 11  LYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPE---AYHIRTLTS 70
           +Y++L   I LN+  S  A S       S+   VHIVY    + ++PE     H R L S
Sbjct: 8   IYVVLSLVIFLNVQRSFVAES-------SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWS 67

Query: 71  VLGSEEAAREALVYSYKNAASGFSARLTPDQVAEIS 104
           +LGS+E A +++VYSY++  SGF+A+LT  Q  +I+
Sbjct: 68  LLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIA 96

[Close]

BLAST of Spg018049 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.4e-05
Identity = 40/114 (35.09%), Postives = 66/114 (57.89%), Query Frame = 0


Query: 11  LYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAY---HIRTLTS 70
           L+L L  SI LN+ +S        A+SK     V++VY      + PE+    H + L S
Sbjct: 6   LFLALFLSIVLNVQISFVV-----AESK-----VYVVYLGEKEHDNPESVTESHHQMLWS 65

Query: 71  VLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQMS 122
           +LGS+EA  +++VYSY++  SGF+A+LT  Q  +IS E  +V+    ++ ++M+
Sbjct: 66  LLGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQQIS-ELPEVVQVIPNTLYEMT 108

[Close]

BLAST of Spg018049 vs. ExPASy Swiss-Prot
Match: Q8GUK4 (Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.3e-05
Identity = 38/103 (36.89%), Postives = 60/103 (58.25%), Query Frame = 0


Query: 4   LNSSSFPLYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPE---AY 63
           +N+S     L+LL +I+L L L++      +A +  S   V+IVY      ++PE   A 
Sbjct: 1   MNNSLQSSKLVLLLAIALVLFLNTELDFLTAAGALDSDSKVYIVYLGEREHDDPELVTAS 60

Query: 64  HIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEIS 104
           H + L S+L S+E A+ +L+YSY++  SGF+A LT  Q  +IS
Sbjct: 61  HHQMLESLLQSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKIS 103

[Close]

BLAST of Spg018049 vs. ExPASy TrEMBL
Match: A0A6J1F5X6 (subtilisin-like protease SBT3.11 OS=Cucurbita moschata OX=3662 GN=LOC111442644 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 7.6e-36
Identity = 94/126 (74.60%), Postives = 105/126 (83.33%), Query Frame = 0


Query: 1   MAKLNSSSFPLYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAY 60
           MAKL  SSFPL  +LLFSISLNL+LSSSAMS QS DS S+SPAVHIVY E+PRDEEPEAY
Sbjct: 1   MAKLIFSSFPLCFVLLFSISLNLILSSSAMSAQSVDSASASPAVHIVYMEKPRDEEPEAY 60

Query: 61  HIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQM 120
           HIRTL SVLGSEEAAREALVYSYKNAASGFSA+LTP+QVAE+S +   VL      ++Q+
Sbjct: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQ-EGVLQVVPSRTYQL 120

Query: 121 -SGGAG 126
            SG AG
Sbjct: 121 HSGPAG 125

[Close]

BLAST of Spg018049 vs. ExPASy TrEMBL
Match: A0A6J1CPA2 (subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013481 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 7.9e-33
Identity = 90/125 (72.00%), Postives = 98/125 (78.40%), Query Frame = 0


Query: 1   MAKLNSSSFPLYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAY 60
           MAK   SSF L L LLFSISLN LLSS+AMSGQ A     SPAVHIVYTE+PRDEEPEAY
Sbjct: 1   MAKTTFSSFLLCLALLFSISLNFLLSSAAMSGQYA-----SPAVHIVYTEKPRDEEPEAY 60

Query: 61  HIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQM 120
           HIRTL SVLGSEEAA+EALVYSYKNAASGFSARLTPDQVAE+S +   VL   +  + Q+
Sbjct: 61  HIRTLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQ-PGVLHVVQSGTHQL 119

Query: 121 SGGAG 126
             G G
Sbjct: 121 HSGGG 119

[Close]

BLAST of Spg018049 vs. ExPASy TrEMBL
Match: A0A6J1F6S6 (subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442643 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 1.3e-30
Identity = 81/104 (77.88%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 6   SSSFPLYLLLLFSISLNLLLSSSAMSGQSAD----SKSSSPAVHIVYTERPRDEEPEAYH 65
           SSSFP  L+LLFSISLNL LSS+ MS QSA     S  +SPAVHIVYTERP +EEPEAYH
Sbjct: 12  SSSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYH 71

Query: 66  IRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISME 106
           IRTL SVLGSEEAAREALVYSYKNAASGFSARLTP+QVA+I+ +
Sbjct: 72  IRTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQ 115

[Close]

BLAST of Spg018049 vs. ExPASy TrEMBL
Match: A0A6J1INL9 (subtilisin-like protease SBT3.10 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477163 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.1e-29
Identity = 85/125 (68.00%), Postives = 97/125 (77.60%), Query Frame = 0


Query: 6   SSSFPLYLLLLFSISLNLLLSSSAMSGQSAD----SKSSSPAVHIVYTERPRDEEPEAYH 65
           SSSFP  L+ +FSI LNL LSS+ MS QSA     S  +SPAVHIVYTERP +EEPEAYH
Sbjct: 12  SSSFPPILVFIFSIYLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYH 71

Query: 66  IRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQM- 125
           IRTL SVLGSEEAAREALVYSYKNAASGFSA+LTP+QVAE+S +   VL      ++Q+ 
Sbjct: 72  IRTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQ-EGVLQVVPSRTYQLH 131

[Close]

BLAST of Spg018049 vs. ExPASy TrEMBL
Match: A0A6J1IH38 (subtilisin-like protease SBT3.6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477163 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 3.1e-29
Identity = 78/104 (75.00%), Postives = 87/104 (83.65%), Query Frame = 0


Query: 6   SSSFPLYLLLLFSISLNLLLSSSAMSGQSAD----SKSSSPAVHIVYTERPRDEEPEAYH 65
           SSSFP  L+ +FSI LNL LSS+ MS QSA     S  +SPAVHIVYTERP +EEPEAYH
Sbjct: 12  SSSFPPILVFIFSIYLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYH 71

Query: 66  IRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISME 106
           IRTL SVLGSEEAAREALVYSYKNAASGFSARLTP+QVA+I+ +
Sbjct: 72  IRTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQ 115

[Close]

BLAST of Spg018049 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 99.4 bits (246), Expect = 3.1e-21
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 0


Query: 11  LYLLLLFSISLNLLLSSSAMSGQ-SADSKSSSPA-VHIVYTERPRDEEPEAYHIRTLTSV 70
           + ++  F   L+   +S  M+ + + ++  SS A VHI+YTE+P DEEP+ YH+RTL+S 
Sbjct: 14  IIIVFFFFFCLSSFFASIVMADEYTGEATGSSEAKVHIIYTEKPTDEEPKTYHLRTLSSA 73

Query: 71  LGSEEAAREALVYSYKNAASGFSARLTPDQVAEISME--FWKVLMHFEHSSFQMSGGAGF 127
           LGSEEAA++AL+YSYK AASGFSA+LTP+QVAEIS +    +V+    +   +  GG GF
Sbjct: 74  LGSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQLHKPGGGGGF 133

[Close]

BLAST of Spg018049 vs. TAIR 10
Match: AT5G11940.1 (Subtilase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 5.1e-08
Identity = 29/63 (46.03%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 44  VHIVYTERPRDEEPE---AYHIRTLTSVLGSEEAAREALVYSYKNAASGFSARLTPDQVA 103
           VHIVY       +PE   + H+R L S+LGS++ A E++V+SY+N  SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

[Close]

BLAST of Spg018049 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 53.1 bits (126), Expect = 2.5e-07
Identity = 29/68 (42.65%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 39  SSSPAVHIVYTERPRDEEPE---AYHIRTLTSVLGSEEAAREALVYSYKNAASGFSARLT 98
           S+   VHIVY    + ++PE     H R L S+LGS+E A +++VYSY++  SGF+A+LT
Sbjct: 45  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 104

Query: 99  PDQVAEIS 104
             Q  +I+
Sbjct: 105 ESQAKKIA 112

[Close]

BLAST of Spg018049 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 51.6 bits (122), Expect = 7.4e-07
Identity = 27/68 (39.71%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 39  SSSPAVHIVYTERPRDEEPE---AYHIRTLTSVLGSEEAAREALVYSYKNAASGFSARLT 98
           S+   VHIVY    + ++P+     H + L+S+LGS++ A E++VYSY++  SGF+A+LT
Sbjct: 24  SAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLT 83

Query: 99  PDQVAEIS 104
             Q  +I+
Sbjct: 84  KSQAKKIA 91

[Close]

BLAST of Spg018049 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 51.2 bits (121), Expect = 9.6e-07
Identity = 40/114 (35.09%), Postives = 66/114 (57.89%), Query Frame = 0


Query: 11  LYLLLLFSISLNLLLSSSAMSGQSADSKSSSPAVHIVYTERPRDEEPEAY---HIRTLTS 70
           L+L L  SI LN+ +S        A+SK     V++VY      + PE+    H + L S
Sbjct: 6   LFLALFLSIVLNVQISFVV-----AESK-----VYVVYLGEKEHDNPESVTESHHQMLWS 65

Query: 71  VLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISMEFWKVLMHFEHSSFQMS 122
           +LGS+EA  +++VYSY++  SGF+A+LT  Q  +IS E  +V+    ++ ++M+
Sbjct: 66  LLGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQQIS-ELPEVVQVIPNTLYEMT 108

The following BLAST results are available for this feature:

BLAST of Spg018049 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022935866.1	1.6e-35	74.60	subtilisin-like protease SBT3.11 [Cucurbita moschata] >XP_023537186.1 subtilisin...	[more]
XP_038897393.1	8.7e-34	81.90	subtilisin-like protease SBT3.17 [Benincasa hispida]	[more]
XP_022143620.1	1.6e-32	72.00	subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia]	[more]
KAG7025065.1	2.6e-30	77.88	Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_023534872.1	2.6e-30	77.88	subtilisin-like protease SBT3.6 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg018049 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1PDX5	7.2e-07	46.03	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]
F4HPF1	1.0e-05	39.71	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q8L7I2	1.4e-05	38.54	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
Q9ZSB0	1.4e-05	35.09	Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S...	[more]
Q8GUK4	2.3e-05	36.89	Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2...	[more]

BLAST of Spg018049 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F5X6	7.6e-36	74.60	subtilisin-like protease SBT3.11 OS=Cucurbita moschata OX=3662 GN=LOC111442644 P...	[more]
A0A6J1CPA2	7.9e-33	72.00	subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1F6S6	1.3e-30	77.88	subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1INL9	1.1e-29	68.00	subtilisin-like protease SBT3.10 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1IH38	3.1e-29	75.00	subtilisin-like protease SBT3.6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Spg018049 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	3.1e-21	48.33	Proteinase inhibitor, propeptide	[more]
AT5G11940.1	5.1e-08	46.03	Subtilase family protein	[more]
AT4G10550.3	2.5e-07	42.65	Subtilase family protein	[more]
AT1G32950.1	7.4e-07	39.71	Subtilase family protein	[more]
AT4G10520.1	9.6e-07	35.09	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 46..104 e-value: 1.2E-7 score: 32.2
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 26..123 e-value: 7.8E-17 score: 63.5
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 19..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg018049.1	Spg018049.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity