Spg017990 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017990
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRibonuclease H
Locationscaffold9: 26929153 .. 26933653 (-)
RNA-Seq ExpressionSpg017990
SyntenySpg017990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGGGGACAGAGATGCGAATGGGTGCATGACTATTGGTTAGAAACAAAAACGGCAGTGCAGAACAGCCTCGCTATAGCGTCGTCTGGGCAACGAGTGATGTCGGATTCTACCCAACAAGTTCAATTAAGCATCAATAATACGTTGTTTGAGAATGAAGTCTTGATGTTTACAGATGCCTCATGCTCTCCATTGGTCCTGGGATCGGGATATGGGGTTATTGTTTTTCAGGCAAATAATCAACTTTGTGGTGCTATGGAAGTTTTCGAACCAACACGTCATAAGCCACTAGCGGCTGAAGTGAATGCACTATTACATGGCCTTCGTTTGTTACACACAATGCAGGTCCGTCGTGCGCGTGTTTTATCAGATTCATCCATTGCAATCAAGATGATTCAGAATGACATTAATATCACCTCTGATGTGTATCATTGGATTTTAAAAATCCAAGATATGTGTGCACTATTTGAGTCAGTATCTTTCCATTATGTGTCTCGACAATGTAATAGGAAGGCAGACTATCTAGCAAAGCATGCTCTTTCTCATGGTAGCTCTACGCTTTGGGTAGGAAACTATCCACAAGAGCTTGTATCAATGAGTAATTTTACTCATTGCAACTGTAATGTTTTGTGTAATTGTGTGATGGAATGAAATGACTTCGATTTTGTTATAAAAAAAAAAAATGAGAGACTAAATTAAAATTTTGTAAATATTAAAATAAAATTTAATAAATAAAAAAGCATAAACGTGCATCACACGTGGTGAAAAACTAGTCAATCCAAATGAATGGTTAAGTTGTATCGAATTAGATTGGTCAAACGTTGACTTTAGGTCGGGCTTAGATCAGGCTCGAGTTGAGCATGAGCGGGCCTTTAGGCCACAAACTCGATGATGAGGCTGATTGCCAACATCATAACTCAACTAAAACATTGTAATCTCAACCAGGAGGTTTGTGGTTAAAATCTCTATCTCCACTTACTTTTTTATTTTTTTTATTTTTTAAGCTTTCATTGTACGCATTCATGCATTAAAAAAGACTTTAGAATTCTTCCAAAACTCACTAATTTCAACTAAGTCGAGTCCTAACAAATCAATGTGCAAAATTAAGTCTATACAAGTAAGCATACAAGATCCACCATTATTGACTCACAAATTTAACAAAAATCTAACTCTAATATCATTTTTTATCCATCAATTTCTTACTAAGTTCCAATTTTCTTTTCATATTCCATTAAAGACGTATGGGAAATTTATTTCAAAATCCTTAAAAGAAAATAAAAAGTTAAGTAAAATGGGATACATTCAACCTTAGACCAACACGAGCATAGTTAACTAAAAGTATTTAATAGGCCAAGAGATCACGGTTCAAATCCTGAGTAATGACCAACAAGAGCATGGCTCAATTGGCATAAAACTTGTATTCCGACCAAGACATCCCCACCACGGATTTTTTTTATTAATAATGTACTTTAATAAAAAAAAAAAAAATTTAGAGGCGTTCTAAAAGTAATTCCAAAAATAGGGTAGCTAAATCGTGTACGTCGGCCGCGATTTCAATAAGTCGTGGGCCGTGGTCACGATTCCGAGGGTAACTCGCGTACCTGGTACGCGACTTTCTTGTTGGCACGCTGACGAGGCATACATCACCAGGTTGGCGATGATTTGGCGCTGACGTCGCGCCAGCGAACTCGTGTACCTGGTACACGATTTTGTTAAGTAAATCGTGTACCAGGTACACGATTTTGTGGTCCATGAAACCCTACCCCACTCGTGAAGCGCAAACCCAGATACCCCCCTCGGTTTTTTCTCCCTTCCTCCGTTTTTAGCTTCGTCCGCCGCCGTTCCAAGTGCCGTCGCCGCCGTTCCGCCGACCTGTGACCTCGCCGCCGCCTGGGTTCCACGCCGTTCAGTTCGCCGCCGCCTAGGTATGTATATATATTTTTTTTTCTTTTTTTCATATAAAAACATTATTTTAGTGATAGATTTTTAGTTTATGGATTATTTTAGTGATAGATTTTTAGTTTGTGGACTGTTTTAGTGCTTTGTATGTTTATGGATTATTTGGAACCGATTTGGATTGTTTTTGGATTGTTTTGGGTCGTTTTTGGACTGATTTGGATCGTTTTTTGACTGATTTGGGTTGTTTGAGTTTTGGACTTAGATATATATTGCTTTAGGTATATTTTGAACCCACTTGGGTTGTTTTTGGATTGATTTGGGTCGTTTTTGGACTGATTTGGGTCGTTTTTGGACTGATTTGGGCTGTTTGATTTGTAGTGGACTTTGTTTGTAATGGATTTTGATTGTAATGGATTATGTTTTCTTTGTAATGAATATTGATTTGTAGTGGACTTTGTTTGTAATGGATTTTGATTGTAATGGATTATGTTTTCTTTGTAATGAATCTTGATTTGTAGTGGACTTTGTTTGTAATGGATTTTTTTTTGTAATGGACTTTGTAATGGATTATGTTTTCTTTGTAATGAATCTTGTTCTATTAGTTGCTAATGGACTTATGTGTTTTTAAATGTTCAGTATAAATATGGAACCTGGCCAATTAACCAAAGACAATTATATTTACAACGTACGCACCGTTCATAGTCGATATGGGATGATCAATCCACTACTATCTTATAATGTCGAAGAAGAGAGGCAGTGGTTAACAGAAATATCAATATAGATCAACGCATCGTGCCTTACGTACAACAAGCCGGATTTTGGGGGGTTGCGCAGATTGGGTTCATGCAACTAAATTGGCATCTTATTACTGCGTTAGTTGAGCGATGGAGGCCAGAAACCCATACATTTCATATGCCTTGTGGAGAGTGTACTGTTACACTCCAAGATGTTGCAGTGCAATTCGAATTACCTATAGATGGAGAACCTGTAACTGGCTCATTGCAATATAATTAGGGTCAGGTATGTGAAGATTTTTTGGGAGTTCGACCACCTGAATTGCATGGATCTAGATTAAGCATTCCATGGTTGGCAGCACAGTTTGCAGAATTGCCCCCCAATGCCGACGAGATCACAATACAGAGGCATGCGAGAGCCTTTATTATGCAATTGATTGGAGGGTTTCTATTTGCTGACAAGTCCAACACACTTGTTCATCTCATGTTTCTTTCACTTTTAGAAAACTTCGAGATAGCTGGACAATATTCTTGGGGTAGTGCATGTCTTGCATGGTTGTATAGAGAATTATGTCGAGCTAGTGGTGCCAGTGCATTAGAGATTGCCGGTCCACTAATACTACTACAAGTTTGGGCGTTCGATCGATTTTCTATTGTCGCACCACTACACGACTTGCCGACCCCTGGAAATTATGCTGGACGCCCACTTAGTGCTCGGTATGTTAAATTTACGTATAATTTAAATTTATCATAAATTTGTATAATAGTATGCTTTAATTGTATTGTACAATGTGTAGATGGAATGGAATTTTGGCTGCATCTGAGCAAACTACAAATTTGTTGATCATGTATCGTATTACATTTGATTGTCTTATGCCCAACTAGGTAAAACAGTAATCAAATTTTTTCTTATTTTAAAAAAGTATACATTAACTTTATTTTCATTTAATAAATTTATCGTTTTATAAATTTCAGATCACATGGACACCATACACACCTGAGATTATGGCCTTACTACCAGACTATTGTCGCACTGGACGAGAGATTTGGTTGACTGTAAGCCCTCTCATATGTTTTCATATTGTAGAGTGACATTACCCAAATCGAGTGCTAAGACAATTTGGAATGCGACAAATGATGCCGCCATATTGCGATACAGTTATAGAGTTACATCAGTTAGACTTGCGAGGTAAGCACGATCACGATTGGCGACATCATCATGCATCCTATATATCACTGTGGCGTGCACGTCGTGATCATTGTGTCCTGGGCGACATAGTAGACATGCCAGTCGTGTCTGATGACTACTTACACTGGTATGATTCAATCACAAGGAGGTATATCACGCGTGAGGGTGCTTATTATGCTTGCACGGTAAATAGTATATACTTTGATTTTGTAATTATTGAATTTCAGTTACAAATATTTTTTTTATTTTAATTATTTTCTTCTTACAGAATAACTTTGTTCGAGATGTACAACATTTTTCTGTGGAACAAAATATACCACAGTTAGGTTCTATGTGTGAGCAATACGTCACGAACATAACTGATATCATCCAACAAACTAGGAGGCTTACCGTTGTTGACACTGATTGGACACGTGCTCGTCGTAGACGCCGTCGACAACAGGCTGCAGACATTCCACAGCAGAATGAAGATGAAGAACCTCCTATAGTTGATGATGACACTTTAGCTCATCAAGTTCAGTCACTAGCAGATCAGACAGACATTGCTTCAAGTTCTGGTCATGGTGACATAACTCAACAAGATATTGATGATGTGATTCATCATCAATCACACATGGAAGCGGCTCAACCTCGTCGTGGAGCTCGTCAGAGAAGACCACGCCGCTGTGGCACTTAG

mRNA sequence

ATGGAGTGGGGACAGAGATGCGAATGGGTGCATGACTATTGGTTAGAAACAAAAACGGCAGTGCAGAACAGCCTCGCTATAGCGTCGTCTGGGCAACGAGTGATGTCGGATTCTACCCAACAAGTTCAATTAAGCATCAATAATACGTTGTTTGAGAATGAAGTCTTGATGTTTACAGATGCCTCATGCTCTCCATTGGTCCTGGGATCGGGATATGGGGTTATTGTTTTTCAGGCAAATAATCAACTTTGTGGTGCTATGGAAGTTTTCGAACCAACACGTCATAAGCCACTAGCGGCTGAAGTGAATGCACTATTACATGGCCTTCGTTTGTTACACACAATGCAGGTCCGTCGTGCGCGTGTTTTATCAGATTCATCCATTGCAATCAAGATGATTCAGAATGACATTAATATCACCTCTGATGTGTATCATTGGATTTTAAAAATCCAAGATATGTGTGCACTATTTGAGTCAGTATCTTTCCATTATGTGTCTCGACAATGTAATAGGAAGGCAGACTATCTAGCAAAGCATGCTCTTTCTCATGGTAGCTCTACGCTTTGGGTTGGCGATGATTTGGCGCTGACGTCGCGCCAGCGAACTCGTGTACCTGCTTCGTCCGCCGCCGTTCCAAGTGCCGTCGCCGCCGTTCCGCCGACCTGTGACCTCGCCGCCGCCTGGGTTCCACGCCGTTCAGTTCGCCGCCGCCTAGGTTCTATGTGTGAGCAATACGTCACGAACATAACTGATATCATCCAACAAACTAGGAGGCTTACCGTTGTTGACACTGATTGGACACGTGCTCGTCGTAGACGCCGTCGACAACAGGCTGCAGACATTCCACAGCAGAATGAAGATGAAGAACCTCCTATAGTTGATGATGACACTTTAGCTCATCAAGTTCAGTCACTAGCAGATCAGACAGACATTGCTTCAAGTTCTGGTCATGGTGACATAACTCAACAAGATATTGATGATGTGATTCATCATCAATCACACATGGAAGCGGCTCAACCTCGTCGTGGAGCTCGTCAGAGAAGACCACGCCGCTGTGGCACTTAG

Coding sequence (CDS)

ATGGAGTGGGGACAGAGATGCGAATGGGTGCATGACTATTGGTTAGAAACAAAAACGGCAGTGCAGAACAGCCTCGCTATAGCGTCGTCTGGGCAACGAGTGATGTCGGATTCTACCCAACAAGTTCAATTAAGCATCAATAATACGTTGTTTGAGAATGAAGTCTTGATGTTTACAGATGCCTCATGCTCTCCATTGGTCCTGGGATCGGGATATGGGGTTATTGTTTTTCAGGCAAATAATCAACTTTGTGGTGCTATGGAAGTTTTCGAACCAACACGTCATAAGCCACTAGCGGCTGAAGTGAATGCACTATTACATGGCCTTCGTTTGTTACACACAATGCAGGTCCGTCGTGCGCGTGTTTTATCAGATTCATCCATTGCAATCAAGATGATTCAGAATGACATTAATATCACCTCTGATGTGTATCATTGGATTTTAAAAATCCAAGATATGTGTGCACTATTTGAGTCAGTATCTTTCCATTATGTGTCTCGACAATGTAATAGGAAGGCAGACTATCTAGCAAAGCATGCTCTTTCTCATGGTAGCTCTACGCTTTGGGTTGGCGATGATTTGGCGCTGACGTCGCGCCAGCGAACTCGTGTACCTGCTTCGTCCGCCGCCGTTCCAAGTGCCGTCGCCGCCGTTCCGCCGACCTGTGACCTCGCCGCCGCCTGGGTTCCACGCCGTTCAGTTCGCCGCCGCCTAGGTTCTATGTGTGAGCAATACGTCACGAACATAACTGATATCATCCAACAAACTAGGAGGCTTACCGTTGTTGACACTGATTGGACACGTGCTCGTCGTAGACGCCGTCGACAACAGGCTGCAGACATTCCACAGCAGAATGAAGATGAAGAACCTCCTATAGTTGATGATGACACTTTAGCTCATCAAGTTCAGTCACTAGCAGATCAGACAGACATTGCTTCAAGTTCTGGTCATGGTGACATAACTCAACAAGATATTGATGATGTGATTCATCATCAATCACACATGGAAGCGGCTCAACCTCGTCGTGGAGCTCGTCAGAGAAGACCACGCCGCTGTGGCACTTAG

Protein sequence

MEWGQRCEWVHDYWLETKTAVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMFTDASCSPLVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHTMQVRRARVLSDSSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKADYLAKHALSHGSSTLWVGDDLALTSRQRTRVPASSAAVPSAVAAVPPTCDLAAAWVPRRSVRRRLGSMCEQYVTNITDIIQQTRRLTVVDTDWTRARRRRRRQQAADIPQQNEDEEPPIVDDDTLAHQVQSLADQTDIASSSGHGDITQQDIDDVIHHQSHMEAAQPRRGARQRRPRRCGT
Homology
BLAST of Spg017990 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 79.3 bits (194), Expect = 7.2e-11
Identity = 53/182 (29.12%), Postives = 93/182 (51.10%), Query Frame = 0

Query: 6    RCEWVHDYWLETKTAVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMFTDASCSP 65
            +CEW+  + L++ +  Q    +++   R  S+    VQ    ++     + + TDA+C  
Sbjct: 1260 KCEWLTPF-LDSHSQAQ----MSNYSPRTQSNHRPVVQYWRPSS--SVSLKLNTDAACRG 1319

Query: 66   LVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHTMQVRRARVLSD 125
                + +G I+  ++  L  A  +  P    PL AE+  +L GL+           V SD
Sbjct: 1320 --ASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEVESD 1379

Query: 126  SSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKADYLAKHALSHGS 185
            S +AI++I+N+I+   D  +W+++IQ +   F  +SF + SRQCNR A  LAK  ++  S
Sbjct: 1380 SLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGLAKWGITSPS 1432

Query: 186  ST 188
            +T
Sbjct: 1440 AT 1432

BLAST of Spg017990 vs. NCBI nr
Match: KAF3572023.1 (hypothetical protein F2Q69_00063094 [Brassica cretica])

HSP 1 Score: 64.3 bits (155), Expect = 2.4e-06
Identity = 43/128 (33.59%), Postives = 73/128 (57.03%), Query Frame = 0

Query: 55  VLMFTDASCSPLVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHT 114
           VL+ TDA+ +  +L +G G  +    N+ C    ++      PL AE  AL   L     
Sbjct: 82  VLIRTDAAWNESLLLAGLGWTI-TTENRSC-KFALYAEFVGSPLVAESLALRAALHKCKD 141

Query: 115 MQVRRARVLSDSSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKAD 174
           M++RR    SDSS+ IK +Q ++ ++ ++Y  +  I ++   F+SV+F ++SR+ NR+AD
Sbjct: 142 MELRRICCESDSSLLIKALQTNV-MSMEIYGIVADINELILAFDSVAFRWISREKNREAD 201

Query: 175 YLAKHALS 183
            LAK+ L+
Sbjct: 202 MLAKNCLA 206

BLAST of Spg017990 vs. NCBI nr
Match: KAG5395350.1 (hypothetical protein IGI04_025313 [Brassica rapa subsp. trilocularis])

HSP 1 Score: 63.9 bits (154), Expect = 3.1e-06
Identity = 52/172 (30.23%), Postives = 81/172 (47.09%), Query Frame = 0

Query: 14  WLETKTAVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMFTDASCSPLVLGSGYG 73
           W+  +T  +   A+A    R +  +TQ+ +         N +   TDAS       +G G
Sbjct: 55  WISAQTHQKPDQALA----RRVQTNTQEAR------RIPNLIRCQTDASWQKDTRRAGLG 114

Query: 74  VIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHTMQVRRARVLSDSSIAIKMI 133
            I +  N  +    E  +     PL AE  A    LR   +      RVLSD+   ++ I
Sbjct: 115 WIFYNGNESILRGSEA-QDFVGSPLIAETLACRSSLRHAISTGFENLRVLSDNQTLVRAI 174

Query: 134 QNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKADYLAKHALSHGS 186
            +++ IT D++  I  I+ + A F S+SF +VSR  N +AD L++ ALSH S
Sbjct: 175 NSELLIT-DIFGIIADIKVLSASFSSISFAFVSRSANEEADSLSRQALSHAS 214

BLAST of Spg017990 vs. NCBI nr
Match: KAF3526751.1 (hypothetical protein F2Q69_00048373 [Brassica cretica])

HSP 1 Score: 62.8 bits (151), Expect = 7.0e-06
Identity = 42/128 (32.81%), Postives = 73/128 (57.03%), Query Frame = 0

Query: 55  VLMFTDASCSPLVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHT 114
           VL+ TDA+ +  +L +G G  +    N+ C    ++      PL AE  AL   L     
Sbjct: 161 VLIRTDAAWNESLLLAGLGWTI-TTENRSC-KFALYAEFVGSPLVAESLALRAALHKCKD 220

Query: 115 MQVRRARVLSDSSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKAD 174
           M++RR    SDSS+ IK +Q ++ ++ ++Y  ++ I ++   F+ V+F ++SR+ NR+AD
Sbjct: 221 MELRRICCESDSSLLIKALQTNV-MSMEIYGIVVDINELILAFDYVAFRWISREKNREAD 280

Query: 175 YLAKHALS 183
            LAK+ L+
Sbjct: 281 MLAKNCLA 285

BLAST of Spg017990 vs. NCBI nr
Match: GAY61101.1 (hypothetical protein CUMW_207140, partial [Citrus unshiu])

HSP 1 Score: 62.4 bits (150), Expect = 9.1e-06
Identity = 50/191 (26.18%), Postives = 84/191 (43.98%), Query Frame = 0

Query: 2   EWGQRCEWVHDYWLET-KTAVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMFTD 61
           +W  R +W+     E  ++ V  + A+  + +RV   + +  QL  N    E  V + TD
Sbjct: 258 KWNARNQWLFKGKRENPQSVVAKAEAVMEAYKRVQPSADKVAQLGWNPPQ-EGFVKINTD 317

Query: 62  ASCSPLVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHTMQVRRA 121
           A+ +     +G G ++   N Q+                AE  A+  GL++     V+  
Sbjct: 318 AATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKDAHVKDV 377

Query: 122 RVLSDSSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKADYLAKHA 181
            + SDS   + ++ N     S++Y  +L+IQ +   F+ VS  Y  R CN  A  LAK A
Sbjct: 378 IMESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDHVSCVYTHRSCNAIAHSLAKLA 437

Query: 182 LSHGSSTLWVG 192
           L    + +W G
Sbjct: 438 LEKCETVVWKG 447

BLAST of Spg017990 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-11
Identity = 53/182 (29.12%), Postives = 93/182 (51.10%), Query Frame = 0

Query: 6    RCEWVHDYWLETKTAVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMFTDASCSP 65
            +CEW+  + L++ +  Q    +++   R  S+    VQ    ++     + + TDA+C  
Sbjct: 1260 KCEWLTPF-LDSHSQAQ----MSNYSPRTQSNHRPVVQYWRPSS--SVSLKLNTDAACRG 1319

Query: 66   LVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHTMQVRRARVLSD 125
                + +G I+  ++  L  A  +  P    PL AE+  +L GL+           V SD
Sbjct: 1320 --ASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEVESD 1379

Query: 126  SSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKADYLAKHALSHGS 185
            S +AI++I+N+I+   D  +W+++IQ +   F  +SF + SRQCNR A  LAK  ++  S
Sbjct: 1380 SLLAIQLIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGLAKWGITSPS 1432

Query: 186  ST 188
            +T
Sbjct: 1440 AT 1432

BLAST of Spg017990 vs. ExPASy TrEMBL
Match: A0A2H5Q972 (Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_207140 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 4.4e-06
Identity = 50/191 (26.18%), Postives = 84/191 (43.98%), Query Frame = 0

Query: 2   EWGQRCEWVHDYWLET-KTAVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMFTD 61
           +W  R +W+     E  ++ V  + A+  + +RV   + +  QL  N    E  V + TD
Sbjct: 258 KWNARNQWLFKGKRENPQSVVAKAEAVMEAYKRVQPSADKVAQLGWNPPQ-EGFVKINTD 317

Query: 62  ASCSPLVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHTMQVRRA 121
           A+ +     +G G ++   N Q+                AE  A+  GL++     V+  
Sbjct: 318 AATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKDAHVKDV 377

Query: 122 RVLSDSSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKADYLAKHA 181
            + SDS   + ++ N     S++Y  +L+IQ +   F+ VS  Y  R CN  A  LAK A
Sbjct: 378 IMESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDHVSCVYTHRSCNAIAHSLAKLA 437

Query: 182 LSHGSSTLWVG 192
           L    + +W G
Sbjct: 438 LEKCETVVWKG 447

BLAST of Spg017990 vs. ExPASy TrEMBL
Match: A0A5B6UDX5 (Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_018998 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 9.8e-06
Identity = 54/213 (25.35%), Postives = 94/213 (44.13%), Query Frame = 0

Query: 3   WGQRCEWVHDYWLETKTAVQNSLAIASSGQRVMS--DSTQQVQLSINNTLFENE------ 62
           WG R   VH      K  ++++  +++  +R M+  D  Q+ +LS+N     N+      
Sbjct: 502 WGSRNRLVH------KKKIESAEELSAGVKRYMAEIDGIQEKKLSLNRIENRNQEELPSK 561

Query: 63  VLMFTDASCSPLVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHT 122
             +F DA+ +     S  G++V      +   M +       P  AE     H ++L+ +
Sbjct: 562 TTIFFDAAFNTRSNKSMAGLVVTDRTGGIVKTMNISNSNVPTPFTAEAYVGYHAVKLVRS 621

Query: 123 MQVRRARVLSDSSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKAD 182
           M  +  R+  DS   IK  Q +    S +   I  IQ++   F  + F ++ R  N+ A 
Sbjct: 622 MGFQTVRIKGDSRTVIKKCQMNKTDKSVISAIIKDIQELSKQFLKIEFQFIDRNKNKMAH 681

Query: 183 YLAKHALSHGSSTLWVGDDL------ALTSRQR 202
            +AK AL  G  T+++G ++      AL SR R
Sbjct: 682 RIAKEALRRG-ETVYLGGEILHNPQDALESRWR 707

BLAST of Spg017990 vs. ExPASy TrEMBL
Match: A0A6P6UCV9 (Ribonuclease H OS=Coffea arabica OX=13443 GN=LOC113709995 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.3e-05
Identity = 51/186 (27.42%), Postives = 86/186 (46.24%), Query Frame = 0

Query: 4   GQRCEWV-----HDYWLETKTAVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMF 63
           G+  +W      HD   + +TA++   A+A       S S  +       T  E   ++F
Sbjct: 316 GRMTKWAVELVEHDIGYQPRTAIKTQ-ALADFLAEGASLSVTEPSSLPEETRLEEPWVLF 375

Query: 64  TDASCSPLVLGSGYGVIVFQ-ANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHTMQV 123
            D + S    GSG G+++      +L  A++   P  +    AE   LL GLR+ H M +
Sbjct: 376 VDGASSK--EGSGAGLLLTSPTEEELTYALKYDFPASNN--EAEYETLLTGLRIAHQMGI 435

Query: 124 RRARVLSDSSIAIKMIQNDINITSDVY-HWILKIQDMCALFESVSFHYVSRQCNRKADYL 183
              RV SDS + +  ++ +     DV   ++ K+++  ALF++     V R  N++AD L
Sbjct: 436 TAIRVRSDSQLVVYQVRGEYEAKEDVMKKYLTKVREAIALFDTFEIERVPRSQNKRADAL 495

BLAST of Spg017990 vs. ExPASy TrEMBL
Match: A0A6P6U043 (Ribonuclease H OS=Coffea arabica OX=13443 GN=LOC113705791 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.9e-05
Identity = 41/129 (31.78%), Postives = 66/129 (51.16%), Query Frame = 0

Query: 56  LMFTDASCSPLVLGSGYGVIVFQ-ANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLLHT 115
           ++F D + S    GSG G+++      +L  A+    P  +    AE  ALL GLR+ H 
Sbjct: 73  VLFVDEASSK--EGSGAGLLLTSPTGEELTYALRFDFPASNN--EAEYEALLRGLRIAHQ 132

Query: 116 MQVRRARVLSDSSIAIKMIQNDINITSDVY-HWILKIQDMCALFESVSFHYVSRQCNRKA 175
           M +   RV SDS + +  ++ +     DV   ++ K+Q+  ALF++     V R  N++A
Sbjct: 133 MGITAIRVRSDSQLVVHQVRGEYETKEDVMKKYLAKVQEAIALFDAFEIERVPRSQNKRA 192

Query: 176 DYLAKHALS 183
           D L+K A S
Sbjct: 193 DALSKLASS 197

BLAST of Spg017990 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 3.7e-05
Identity = 49/175 (28.00%), Postives = 88/175 (50.29%), Query Frame = 0

Query: 15  LETKT-AVQNSLAIASSGQRVMSDSTQQVQLSINNTLFENEVLMFTDASC--SPLVLGSG 74
           +ET T AVQ++LA   S Q  +           + T   ++ L + DA+      + GSG
Sbjct: 120 IETVTKAVQDALA-WQSAQLALPKVRVATPRPNSTTTLTHDFLCYVDAAWQQQSSLAGSG 179

Query: 75  YGVIVFQANNQLCGAMEVFEPTRHK---PLAAEVNALLHGLRLLHTMQVRRA--RVLSDS 134
           +   VFQA +     +  F     +   PLAAE  A+     +LH +Q+ R+   VLSDS
Sbjct: 180 W---VFQATSHSEKEITTFSAGCRRFPSPLAAEAWAIKSA--MLHALQLERSDLLVLSDS 239

Query: 135 SIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRKADYLAKHAL 182
              +  + +++++ ++++  +++I+ +   F S+SF ++ R  N  AD  AK +L
Sbjct: 240 KSIVDALNSNVSL-NEIFGLLVEIRSIRNRFRSISFQFIPRLVNSIADAAAKLSL 287

BLAST of Spg017990 vs. TAIR 10
Match: AT4G09490.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.8e-04
Identity = 36/129 (27.91%), Postives = 59/129 (45.74%), Query Frame = 0

Query: 53  NEVLMFTDASCSPLVLGSGYGVIVFQANNQLCGAMEVFEPTRHKPLAAEVNALLHGLRLL 112
           + V +FTDA+        G+G ++           +        PL AE  AL   L+  
Sbjct: 29  DRVTIFTDAAWKHETKDVGFGWVIRNCPELTALHYQSAARNVRLPLMAEAIALFLALQYA 88

Query: 113 HTMQVRRARVLSDSSIAIKMIQNDINITSDVYHWILKIQDMCALFESVSFHYVSRQCNRK 172
            ++ + +  + SDS   I  I ++ + +++ Y  I  I ++   F  VSF +V R  NR 
Sbjct: 89  QSIGITKLSMASDSQQLITAITSE-SPSTEFYGIIFDILNLSLGFADVSFSFVPRSENRV 148

Query: 173 ADYLAKHAL 182
           AD LAK +L
Sbjct: 149 ADELAKSSL 156

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.17.2e-1129.12uncharacterized protein LOC111024874 [Momordica charantia][more]
KAF3572023.12.4e-0633.59hypothetical protein F2Q69_00063094 [Brassica cretica][more]
KAG5395350.13.1e-0630.23hypothetical protein IGI04_025313 [Brassica rapa subsp. trilocularis][more]
KAF3526751.17.0e-0632.81hypothetical protein F2Q69_00048373 [Brassica cretica][more]
GAY61101.19.1e-0626.18hypothetical protein CUMW_207140, partial [Citrus unshiu][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DX303.5e-1129.12uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A2H5Q9724.4e-0626.18Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_207140 PE=4... [more]
A0A5B6UDX59.8e-0625.35Reverse transcriptase OS=Gossypium australe OX=47621 GN=EPI10_018998 PE=4 SV=1[more]
A0A6P6UCV91.3e-0527.42Ribonuclease H OS=Coffea arabica OX=13443 GN=LOC113709995 PE=4 SV=1[more]
A0A6P6U0434.9e-0531.78Ribonuclease H OS=Coffea arabica OX=13443 GN=LOC113705791 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G10000.13.7e-0528.00Ribonuclease H-like superfamily protein [more]
AT4G09490.11.8e-0427.91Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 68..180
e-value: 7.6E-18
score: 64.5
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 51..182
score: 11.627664
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 53..186
e-value: 9.9E-20
score: 72.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..354
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 270..292
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 55..183

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017990.1Spg017990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity