Spg017894 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017894
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGrpE protein homolog
Locationscaffold9: 35878953 .. 35881647 (-)
RNA-Seq ExpressionSpg017894
SyntenySpg017894
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTGAGTTAAGTGTGCTCGACTCACTTTAGCATGTTGAAGTTCATTTTGGCTTCCGTAAGACCTAAGAAAGTATCTATCGTCATCAATATTTTATTCATATTTGGATCAAATTGAGACCTCGACATTTTTGTCTCGGCAAATACGTCAAATCTAAGGCCACCGGAAGCCCTCTCTCTCCGAGCAGCCGCCTTCATTTTCTCCAATTCCTTTCCTTTCGTCGGAACCTGTGCCGTTGAATCTCTGTAATTTTGAAGCATTTGATTTCCTTTCACAATCCACCGTCCGCCATTTCAGTTTCTTCTTCTCAATGTCCGTTTCCAGGATCCTCTTCCGAGCTTCCAGGAGCCTGCAGCGGAGCTCGCCGGTCCAATCACAGAACCTGCAAACTTTTCCGATTCTCTGCAGGCAGTTTCACGGTCTCGTTCATGACATCTCCGGCAAGGTTGAGGTTCTAAAGCCGTGTTTTCAAGGTTCTCTCCTCTGGGGTCTTAGTTAGGGTTTTTGCCATTGCATTGGAAAAGGCACTGTTTGATTCTGATTTTGGTTTCTTAGTTATCGTATGCCCTTTTGAAGCCTTTTGTTTTGCTTGCGATTGCGATATCTTTGGATTTAAGCTCACATTATTCTTCGTGAAATCATTGCTTCCCGTTCTATTGGGTTTTAATTGAAAAATCGAGAGGTGATTTGATCTGGGTATGTTTGAACAGTTTGAACTGGTGAAATTTTGCTTCAATGTTGTTGATGTAGCGTTGTCTATTTAACATGTTTTTGCATTTCTCATCATGTTTTATGTTAAATTATTGATATAAGATCAAATTTATCATAACCCATCAGCTTAATCTTTTGGGTTGATTGGTGATTTAACATCTTAAATTTTGGTTCAGCTTTCTCCCTTGAAGCACTTTTTTTTTCCCTTTTCCCTAGCTTGCTTGTTTTAGGATTCAAGGTTGTTTTTCCTGTTACTGAGTTATATGCTTCATCTTCCAGGTGTCTCTATTTCATCATATAGTCTTCAATCCATTCCTCTCCCAAAAGTTTGGAGTATCTTCTTCTTCTGCTTCCCATGAATCTTCTGAAAATGAAGAGGGGAGTGCCAAACAAGGTAATAGTGATCCTGCAAGAGATGAGAATTTAAAAGTTTCTGAACAAGTAGAGGAATTGAATTCTAGGTTGAACTCTCATTCCACTGAATCAAAATCAAGATGGCGCAGTAGGCGCACCATACGAACTGCATTTTCTGATTCGGATTCTGAGAGTGATGAACAACCTTCCATGGATGATCTAGTGAAATCACTGGCGCAGAAGGAAGAGCTTTTGAAGGCTAAGCACAAAGAGATTGAGATAATGCAAGACAAAGTGCTCCGTTCTTATGCAGAAATGGAGAATGTTATGGACAGGACAAGACGTGAAGCAGAGAATTCAAAGAAGTTTGCCATTCAGGTTTCTTGAGTTGACATTTCTTCTAGTGATATATATATGTTCTTGTTTCTTTCTCAAACTCGGGTTTCGTTCCAATGTTCTGCTTGTTCAACATGAACTTATATGTCTTTGCATATGCTTTACAGAATTTTGCAAAGAGTTTGCTTGATGTTGCTGACAACTTGGGTAGAGCTTCTTCTGTGGTTAAAGACAGTTTCTCAAAAATTAATGAATCTGAGGATTCAAATGGAGCTGTACCTCTCCTAAAAACCCTTCTAGAAGGGGTCGACATGACCGAGAAACAGCTTATGGAGGTAAATTTTGTATGTTAGCATAAGCTATTCAACATTTCAAGAAGAAATCACAAGTTAAATCTATTGTTCTGACGCTTAAACACACTTTTTCCACAGGTATTTAAGAAGTCCGGGGTAGAGAAATTCGACCCTACAAACGAGCCATTCGATCCGCATAAGCATAATGCAGTATTCCAAATACCAGATGGATCTAAGCCAGAGGGCACTGTTGCTATTGTTCTGAAGGTATTAGTTCTATTTGCCATCATGCTTCTTCATACTTCTGAAATGTGTGTACTTCAAAAACCATGTGGCAGTTGGAGATATTTAGTTCTTATGCCAATCGAAATTCATTTACCAAACTTTAGGGGGAGAATTTGCTTTTAGAGCAAGCTATAAACAAAATTAGTACTTTTTAAGTTTGATCTTTACTCTTTAGTAGGAGCAGACATGTTTTTTTACATTGTAACAAATATTTCCATCCAACTTTTTTCAAGAACACGAAATCATCACTGATTTGTATTGTAGTTTGGTTTGATTTTAGACACTGCATTTGTTGTGGAGATGATATGATTTTCTCATGTTCTCTATGACAGTCTGGATATATGCTATACGATCGAGTTCTTCGGCCAGCTGAAGTCGGTGTGACCCAAGCCGTGGAAAATAGTTCGATCGATAATGTCTAAAGACATAGAAGCTGTGCTATAGAAGGCTCCCAAGAATATGATTCTGGCTGTCATTGGATTCTTCAAATTCACATTTCTATGACGGAGAATGTTGTATGAGGTACAAAGCAATGAGTCCATCTTTCCAATAGAATTAGAAGAACTTATTTTTGTTTTGTTGAAAATAATAGCATTGAATTATTTGTAAATCTAACCTAATCTTGATGGTTAGAAGGAATTAGTTGCCAGTTTCCATCAGGTTATGTTTTATTGCAAGAAGGATATTTGCTCCATAATAGCCACAAAAAGTATATGCA

mRNA sequence

ATGTCCGTTTCCAGGATCCTCTTCCGAGCTTCCAGGAGCCTGCAGCGGAGCTCGCCGGTCCAATCACAGAACCTGCAAACTTTTCCGATTCTCTGCAGGCAGTTTCACGGTCTCGTTCATGACATCTCCGGCAAGGTTGAGGTTCTAAAGCCGTGTTTTCAAGTTATCGTATGCCCTTTTGAAGCCTTTTGTTTTGCTTGCGATTGCGATATCTTTGGATTTAAGCTCACATTATTCTTCGTGTCTCTATTTCATCATATAGTCTTCAATCCATTCCTCTCCCAAAAGTTTGGAGTATCTTCTTCTTCTGCTTCCCATGAATCTTCTGAAAATGAAGAGGGGAGTGCCAAACAAGGTAATAGTGATCCTGCAAGAGATGAGAATTTAAAAGTTTCTGAACAAGTAGAGGAATTGAATTCTAGGTTGAACTCTCATTCCACTGAATCAAAATCAAGATGGCGCAGTAGGCGCACCATACGAACTGCATTTTCTGATTCGGATTCTGAGAGTGATGAACAACCTTCCATGGATGATCTAGTGAAATCACTGGCGCAGAAGGAAGAGCTTTTGAAGGCTAAGCACAAAGAGATTGAGATAATGCAAGACAAAGTGCTCCGTTCTTATGCAGAAATGGAGAATGTTATGGACAGGACAAGACGTGAAGCAGAGAATTCAAAGAAGTTTGCCATTCAGAATTTTGCAAAGAGTTTGCTTGATGTTGCTGACAACTTGGGTAGAGCTTCTTCTGTGGTTAAAGACAGTTTCTCAAAAATTAATGAATCTGAGGATTCAAATGGAGCTGTACCTCTCCTAAAAACCCTTCTAGAAGGGGTCGACATGACCGAGAAACAGCTTATGGAGGTATTTAAGAAGTCCGGGGTAGAGAAATTCGACCCTACAAACGAGCCATTCGATCCGCATAAGCATAATGCAGTATTCCAAATACCAGATGGATCTAAGCCAGAGGGCACTGTTGCTATTGTTCTGAAGTCTGGATATATGCTATACGATCGAGTTCTTCGGCCAGCTGAAGTCGGTGTGACCCAAGCCGTGGAAAATAGTTCGATCGATAATGTCTAA

Coding sequence (CDS)

ATGTCCGTTTCCAGGATCCTCTTCCGAGCTTCCAGGAGCCTGCAGCGGAGCTCGCCGGTCCAATCACAGAACCTGCAAACTTTTCCGATTCTCTGCAGGCAGTTTCACGGTCTCGTTCATGACATCTCCGGCAAGGTTGAGGTTCTAAAGCCGTGTTTTCAAGTTATCGTATGCCCTTTTGAAGCCTTTTGTTTTGCTTGCGATTGCGATATCTTTGGATTTAAGCTCACATTATTCTTCGTGTCTCTATTTCATCATATAGTCTTCAATCCATTCCTCTCCCAAAAGTTTGGAGTATCTTCTTCTTCTGCTTCCCATGAATCTTCTGAAAATGAAGAGGGGAGTGCCAAACAAGGTAATAGTGATCCTGCAAGAGATGAGAATTTAAAAGTTTCTGAACAAGTAGAGGAATTGAATTCTAGGTTGAACTCTCATTCCACTGAATCAAAATCAAGATGGCGCAGTAGGCGCACCATACGAACTGCATTTTCTGATTCGGATTCTGAGAGTGATGAACAACCTTCCATGGATGATCTAGTGAAATCACTGGCGCAGAAGGAAGAGCTTTTGAAGGCTAAGCACAAAGAGATTGAGATAATGCAAGACAAAGTGCTCCGTTCTTATGCAGAAATGGAGAATGTTATGGACAGGACAAGACGTGAAGCAGAGAATTCAAAGAAGTTTGCCATTCAGAATTTTGCAAAGAGTTTGCTTGATGTTGCTGACAACTTGGGTAGAGCTTCTTCTGTGGTTAAAGACAGTTTCTCAAAAATTAATGAATCTGAGGATTCAAATGGAGCTGTACCTCTCCTAAAAACCCTTCTAGAAGGGGTCGACATGACCGAGAAACAGCTTATGGAGGTATTTAAGAAGTCCGGGGTAGAGAAATTCGACCCTACAAACGAGCCATTCGATCCGCATAAGCATAATGCAGTATTCCAAATACCAGATGGATCTAAGCCAGAGGGCACTGTTGCTATTGTTCTGAAGTCTGGATATATGCTATACGATCGAGTTCTTCGGCCAGCTGAAGTCGGTGTGACCCAAGCCGTGGAAAATAGTTCGATCGATAATGTCTAA

Protein sequence

MSVSRILFRASRSLQRSSPVQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIVCPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAKQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQPSMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENSSIDNV
Homology
BLAST of Spg017894 vs. NCBI nr
Match: XP_023536001.1 (grpE protein homolog 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 473.4 bits (1217), Expect = 1.7e-129
Identity = 273/358 (76.26%), Postives = 299/358 (83.52%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPV----QSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVI 60
           MS S ILFRASRSL RSSP+    QSQN +TFPIL RQFHGLV +ISGKVEVLKPCFQ  
Sbjct: 1   MSSSSILFRASRSLHRSSPILIGNQSQNCRTFPILYRQFHGLVDEISGKVEVLKPCFQG- 60

Query: 61  VCPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSA 120
                          F F+     VSL +HIVFNPF  Q+FG+ SS+ASHESSENEE SA
Sbjct: 61  ---------------FLFQSAPVHVSLLNHIVFNPFPFQRFGL-SSTASHESSENEERSA 120

Query: 121 KQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQPSM 180
           KQG        N +VS Q EEL+S  +SHST+SKSR + RRTIRTAFS+SDSESDEQPSM
Sbjct: 121 KQGT------VNSEVSNQAEELSSTTDSHSTKSKSRRQRRRTIRTAFSNSDSESDEQPSM 180

Query: 181 DDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKS 240
           DDLVKSL +KEELLK+KHKEIE MQ+KVL SYAEMENVMDRT+REAENSKKFAIQNFAKS
Sbjct: 181 DDLVKSLTRKEELLKSKHKEIEKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKS 240

Query: 241 LLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEK 300
           LLDVADNLGRASSVVKDSFSKI+ESED +GAV LLKTLLEGVDMTEKQLMEVFKKSGVEK
Sbjct: 241 LLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEK 300

Query: 301 FDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENS 355
           FDPT+EPFDPHKH AVFQIPDGSKP+GTVA+VLKSGYML+DRVLRPAEVGVTQAVE++
Sbjct: 301 FDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDN 335

BLAST of Spg017894 vs. NCBI nr
Match: KAG7024936.1 (grpE, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 453.0 bits (1164), Expect = 2.4e-123
Identity = 265/361 (73.41%), Postives = 291/361 (80.61%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPV----QSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVI 60
           MS  RILFRASRSL +SSP+    QSQN +TFPIL RQFHGLV +ISGKVE         
Sbjct: 1   MSSFRILFRASRSLHQSSPILIGNQSQNCRTFPILYRQFHGLVDEISGKVESAP------ 60

Query: 61  VCPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSA 120
                                   VSL +HIV NPF  Q+ GV SS+ASHESSENEEGSA
Sbjct: 61  ----------------------VHVSLLNHIVSNPFPFQRLGV-SSTASHESSENEEGSA 120

Query: 121 KQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQPSM 180
           KQ N       N +VS Q EEL+S  +SHST+SKSR + RRTIRTAFS+SDSESDEQPSM
Sbjct: 121 KQDN------VNSEVSNQAEELSSTTDSHSTKSKSRRQRRRTIRTAFSESDSESDEQPSM 180

Query: 181 DDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKS 240
           DDLVKSLA+KEELLK+KHKEI+ MQ+KVL SYAEMENVMDRT+REAENSKKFAIQNFAKS
Sbjct: 181 DDLVKSLARKEELLKSKHKEIDKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKS 240

Query: 241 LLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEK 300
           LLDVADNLGRASSVVKDSFSKI+ESED +GAV LLKTLLEGVDMTEKQLMEVFKKSGVEK
Sbjct: 241 LLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEK 300

Query: 301 FDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENSSI 358
           FDPT+EPFDPHKH AVFQIPDGSKP+GTVA+VLKSGYML+DRVLRPAEVGVTQAVE++S 
Sbjct: 301 FDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNST 326

BLAST of Spg017894 vs. NCBI nr
Match: XP_038898199.1 (grpE protein homolog 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 451.8 bits (1161), Expect = 5.4e-123
Identity = 262/358 (73.18%), Postives = 290/358 (81.01%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPVQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIVCPF 60
           MSVSRILFRASRSL RSSP+     QTFPIL RQFHGLVH+ISGK   ++          
Sbjct: 39  MSVSRILFRASRSLHRSSPI-LYGYQTFPILYRQFHGLVHEISGKSAPVQ---------- 98

Query: 61  EAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAKQG- 120
                               VSL +HIV+NPF  Q+FGV SS+A HESSEN EGSAK   
Sbjct: 99  --------------------VSLLNHIVYNPFPFQRFGV-SSTAIHESSEN-EGSAKHSG 158

Query: 121 --NSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQPSMD 180
             N+ P +  N +VS++ EE    L+SHST+SKSR RSRR++RTAFSDSDSESDEQPSMD
Sbjct: 159 STNNGPTKHGNSEVSDRAEESTFTLDSHSTKSKSRRRSRRSVRTAFSDSDSESDEQPSMD 218

Query: 181 DLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKSL 240
           DLVKSLA+KEELLK+KHKE+E MQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKSL
Sbjct: 219 DLVKSLARKEELLKSKHKELEKMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKSL 278

Query: 241 LDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEKF 300
           LDVADNLGRASSVVKDSFSKINES+DS+GAVPLLK+LLEGVDMTEKQLME F K GVEKF
Sbjct: 279 LDVADNLGRASSVVKDSFSKINESQDSSGAVPLLKSLLEGVDMTEKQLMEAFTKFGVEKF 338

Query: 301 DPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENSS 356
           DPT+EPFDPHKH AVFQIPDGSKP+GTVAIVLKSGYML+DRVLRPAEVGVTQA E++S
Sbjct: 339 DPTSEPFDPHKHFAVFQIPDGSKPDGTVAIVLKSGYMLHDRVLRPAEVGVTQAAEDNS 363

BLAST of Spg017894 vs. NCBI nr
Match: XP_022936267.1 (grpE protein homolog 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 449.9 bits (1156), Expect = 2.1e-122
Identity = 264/361 (73.13%), Postives = 289/361 (80.06%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPV----QSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVI 60
           MS  RIL RASRSL +SSP+    QSQN +TFPIL RQFHGLV +ISGK   +       
Sbjct: 1   MSSFRILVRASRSLHQSSPILIRNQSQNCRTFPILYRQFHGLVDEISGKSAPV------- 60

Query: 61  VCPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSA 120
                                   VSL +HIV NPF  Q+FGV SS+ASHESSENEEGSA
Sbjct: 61  -----------------------HVSLLNHIVSNPFPFQRFGV-SSTASHESSENEEGSA 120

Query: 121 KQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQPSM 180
           KQ       D N +VS Q EEL+S  +SHST+SKSR + RRTIRTAFSDSDSE DEQPSM
Sbjct: 121 KQD------DVNSEVSNQAEELSSTTDSHSTKSKSRRQRRRTIRTAFSDSDSECDEQPSM 180

Query: 181 DDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKS 240
           DDLVKSLA+KEELLK+KHKEIE MQ+KVL SYAEMENVMDRT+REAENSKKFAIQNFAKS
Sbjct: 181 DDLVKSLARKEELLKSKHKEIEKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKS 240

Query: 241 LLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEK 300
           LLDVADNLGRASSVVKDSFSKI+ESED +GAV LLKTLLEGVDMTEKQLMEVFKKSGVEK
Sbjct: 241 LLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEK 300

Query: 301 FDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENSSI 358
           FDPT+EPFDPHKH AVFQIPDGSKP+GTVA+VLKSGYML+DRVLRPAEVGVTQAVE++S 
Sbjct: 301 FDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNST 324

BLAST of Spg017894 vs. NCBI nr
Match: KAG6592058.1 (Transcriptional adapter ADA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 449.1 bits (1154), Expect = 3.5e-122
Identity = 263/361 (72.85%), Postives = 290/361 (80.33%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPV----QSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVI 60
           MS  RILFRASRSL +SSP+    QSQN +TFPIL RQFHGLV +ISGK   +       
Sbjct: 587 MSSFRILFRASRSLHQSSPILIGNQSQNCRTFPILYRQFHGLVDEISGKSAPV------- 646

Query: 61  VCPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSA 120
                                   VSL +HIV NPF  Q+ GV SS+ASHESSENEEGSA
Sbjct: 647 -----------------------HVSLLNHIVSNPFPFQRLGV-SSTASHESSENEEGSA 706

Query: 121 KQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQPSM 180
           KQ N       N +VS Q EEL+S  +SHST+SKSR + RRTIRTAFSDSDSES+EQPSM
Sbjct: 707 KQDN------VNSEVSNQAEELSSTTDSHSTKSKSRRQRRRTIRTAFSDSDSESNEQPSM 766

Query: 181 DDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKS 240
           DDLVKSLA+KEELLK+KHKEI+ MQ+KVL SYAEMENVMDRT+REAENSKKFAIQNFAKS
Sbjct: 767 DDLVKSLARKEELLKSKHKEIDKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKS 826

Query: 241 LLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEK 300
           LLDVADNLGRASSVVKDSFSKI+ESED +GAV LLKTLLEGVDMTEKQLMEVFKKSGVEK
Sbjct: 827 LLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEK 886

Query: 301 FDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENSSI 358
           FDPT+EPFDPHKH AVFQIPDGSKP+GTVA+VLKSGYML+DRVLRPAEVGVTQAVE++S 
Sbjct: 887 FDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNST 910

BLAST of Spg017894 vs. ExPASy Swiss-Prot
Match: Q8LB47 (GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE=1 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 5.7e-75
Identity = 184/360 (51.11%), Postives = 234/360 (65.00%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPVQSQNL---QTFPILCRQFHGLVHDISGKVEVLKPCFQVIV 60
           M V RIL R +R+    S + +  L      P+   +FH L HD S K   L P      
Sbjct: 1   MLVLRILSRVTRNAGIRSSLSAVTLPARNQTPVFSSRFHSLAHDFSHK---LVPA----- 60

Query: 61  CPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAK 120
                                         + + F  Q+F  SSS+    S E++E   K
Sbjct: 61  ---------------------------QMSMMDSFALQRFNFSSST----SPESDE---K 120

Query: 121 QGNSDPARDENLKVSEQVEE--LNSRLNSHSTESKSRWR-SRRTIRTAFSDSDSES-DEQ 180
           + +++ ++    K + +  +  L+S      T+S  R R   +   ++ S+SDSES D++
Sbjct: 121 KTHTEASKTSEEKPTAEANQPGLDSESKDSVTDSAKRKRKGAKGAASSSSESDSESDDDE 180

Query: 181 PSMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNF 240
            S DDLVK +A+KEELL  K +EI+ ++DKVLR+YAEMENVMDRTRR+AEN+KK+A+QNF
Sbjct: 181 LSADDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAEMENVMDRTRRDAENTKKYAVQNF 240

Query: 241 AKSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSG 300
           AKSLLDVADNLGRASSVVK+SFSK++ SEDS GA PLLKTLLEGV+MTEKQL EVFKK G
Sbjct: 241 AKSLLDVADNLGRASSVVKESFSKLDTSEDSAGAAPLLKTLLEGVEMTEKQLAEVFKKFG 300

Query: 301 VEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVEN 354
           +EK+DP NEPFDP++HNAVFQ+PD SKPEGTVA VLKSGY LYDRV+RPAEVGVTQ  EN
Sbjct: 301 MEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRVIRPAEVGVTQGGEN 318

BLAST of Spg017894 vs. ExPASy Swiss-Prot
Match: Q9FLP3 (GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 5.4e-65
Identity = 170/358 (47.49%), Postives = 218/358 (60.89%), Query Frame = 0

Query: 1   MSVSRILFRASRS--LQRS-SPVQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIV 60
           M VSR+L R SRS  L+ S S V +      PI+  +FH LVH    K         ++ 
Sbjct: 1   MLVSRVLSRVSRSAGLRSSFSSVVTPKRNQIPIVASRFHSLVHGTPNK---------LVA 60

Query: 61  CPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFG-VSSSSASHESSENEEGSA 120
            P                     VSL +H   +  + Q+FG  SSSSA  + +E+     
Sbjct: 61  VP---------------------VSLRNHGTLDLNVLQRFGFFSSSSAEPKGNESNTEVP 120

Query: 121 KQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESD-EQPS 180
           K G +     EN++V +                                +D+E D +  S
Sbjct: 121 KTGET----SENVEVGKA-------------------------------TDAEIDFDDLS 180

Query: 181 MDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAK 240
            DDLVK +++KE+LLK + K+I  M+DK LR+YAE +N+MDRT R AE++KKFA+QNFA 
Sbjct: 181 RDDLVKLVSEKEDLLKVQQKDIMEMKDKFLRTYAEQQNLMDRTNRNAESAKKFAVQNFAT 240

Query: 241 SLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVE 300
           SLLDVADNL RASSVVK+SFSKI+ S+D  GA PLLK LLEGV+MTEKQL EVF+K+G+ 
Sbjct: 241 SLLDVADNLERASSVVKESFSKIDTSKDLAGATPLLKNLLEGVEMTEKQLAEVFRKAGLV 293

Query: 301 KFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVEN 354
           K DP NEPF+P++HNAVFQ+PD SKP+GT+A VLKSGY LYDRV+RPAEVGVT AVEN
Sbjct: 301 KEDPLNEPFNPNRHNAVFQVPDASKPKGTIAHVLKSGYSLYDRVIRPAEVGVTCAVEN 293

BLAST of Spg017894 vs. ExPASy Swiss-Prot
Match: A4YJR1 (Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 6.2e-29
Identity = 70/159 (44.03%), Postives = 106/159 (66.67%), Query Frame = 0

Query: 191 KAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSV 250
           +A  KE    +DK+LR+ AEMEN+  RT RE  +++ + I  FA+ +LD+ADNL RA   
Sbjct: 38  EAYAKEAAEARDKMLRTLAEMENLRKRTAREVADARMYGITGFARDVLDIADNLQRALDA 97

Query: 251 VKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTNEPFDPHKHN 310
           V         +E    A   LK+L+EGV++TE+ L+   +K+GV+KFDPT + FDP+   
Sbjct: 98  V--------PAETRANADAGLKSLIEGVELTERSLLNTLEKNGVKKFDPTGQKFDPNFQQ 157

Query: 311 AVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQ 350
           A++++PD S P GTV  V+++G+M+ +RVLRPA VGV++
Sbjct: 158 AMYEVPDPSVPSGTVVQVVQAGFMIGERVLRPALVGVSK 188

BLAST of Spg017894 vs. ExPASy Swiss-Prot
Match: Q3SW78 (Protein GrpE OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=grpE PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 6.2e-29
Identity = 78/196 (39.80%), Postives = 119/196 (60.71%), Query Frame = 0

Query: 164 SDSDSESDEQPSMDDLVKSLAQK---------EELLKAKHKEIEIMQDKVLRSYAEMENV 223
           +DSD ++D+       V+ +  K         ++ L A +K++   +D+ LR+ AEMEN+
Sbjct: 2   TDSDGKTDKSGEPAAEVEPVVSKPYVMPDDPEDDALDALNKQLAEAKDRTLRTLAEMENL 61

Query: 224 MDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKINESEDSNGAV-PLLKT 283
             RT RE  +++ + I  FA+ +L++ADNL RA   V           D+  A  P LK 
Sbjct: 62  RKRTAREVSDARTYGISGFARDVLEIADNLQRALDAV---------PADARAAPDPGLKA 121

Query: 284 LLEGVDMTEKQLMEVFKKSGVEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGY 343
           L+EGV++TE+ L    +K GV+KFDP  E FDP+ H A++++PD S P GTVA V+++GY
Sbjct: 122 LIEGVELTERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIPVGTVAQVIQAGY 181

Query: 344 MLYDRVLRPAEVGVTQ 350
           M+ +RVLRPA VGV +
Sbjct: 182 MIGERVLRPALVGVAK 188

BLAST of Spg017894 vs. ExPASy Swiss-Prot
Match: B6JCI1 (Protein GrpE OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) OX=504832 GN=grpE PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 6.2e-29
Identity = 70/156 (44.87%), Postives = 102/156 (65.38%), Query Frame = 0

Query: 195 KEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDS 254
           KE    +D++LR+ AEMEN+  RT+RE  +++ + I  FA+ +L++ADNL RA   V   
Sbjct: 47  KEAAEAKDRMLRTLAEMENLRKRTQREVADARAYGIAGFARDVLEIADNLQRALDAV--- 106

Query: 255 FSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTNEPFDPHKHNAVFQ 314
                 +E    A P L  L+EGV++TE+ L    +K+GV+K D   E FDP+ H A+F+
Sbjct: 107 -----PAEARAAADPGLTALIEGVELTERSLHRALEKNGVKKLDAAGEKFDPNIHQAMFE 166

Query: 315 IPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQA 351
           +PD S P GTV  V+++GYM+ DRVLRPA VGV++A
Sbjct: 167 VPDNSVPPGTVVQVIQTGYMIGDRVLRPALVGVSKA 194

BLAST of Spg017894 vs. ExPASy TrEMBL
Match: A0A6J1F7Z2 (GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111442938 PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 1.0e-122
Identity = 264/361 (73.13%), Postives = 289/361 (80.06%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPV----QSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVI 60
           MS  RIL RASRSL +SSP+    QSQN +TFPIL RQFHGLV +ISGK   +       
Sbjct: 1   MSSFRILVRASRSLHQSSPILIRNQSQNCRTFPILYRQFHGLVDEISGKSAPV------- 60

Query: 61  VCPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSA 120
                                   VSL +HIV NPF  Q+FGV SS+ASHESSENEEGSA
Sbjct: 61  -----------------------HVSLLNHIVSNPFPFQRFGV-SSTASHESSENEEGSA 120

Query: 121 KQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQPSM 180
           KQ       D N +VS Q EEL+S  +SHST+SKSR + RRTIRTAFSDSDSE DEQPSM
Sbjct: 121 KQD------DVNSEVSNQAEELSSTTDSHSTKSKSRRQRRRTIRTAFSDSDSECDEQPSM 180

Query: 181 DDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAKS 240
           DDLVKSLA+KEELLK+KHKEIE MQ+KVL SYAEMENVMDRT+REAENSKKFAIQNFAKS
Sbjct: 181 DDLVKSLARKEELLKSKHKEIEKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAKS 240

Query: 241 LLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVEK 300
           LLDVADNLGRASSVVKDSFSKI+ESED +GAV LLKTLLEGVDMTEKQLMEVFKKSGVEK
Sbjct: 241 LLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVEK 300

Query: 301 FDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENSSI 358
           FDPT+EPFDPHKH AVFQIPDGSKP+GTVA+VLKSGYML+DRVLRPAEVGVTQAVE++S 
Sbjct: 301 FDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNST 324

BLAST of Spg017894 vs. ExPASy TrEMBL
Match: A0A6J1CCY7 (GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111010351 PE=3 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.8e-122
Identity = 262/365 (71.78%), Postives = 289/365 (79.18%), Query Frame = 0

Query: 1   MSVSRILFRAS-RSLQRSSPVQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIVCP 60
           MSVSR+LFRAS RSL+RSS          PIL +QF GLVH+ SGK   ++         
Sbjct: 1   MSVSRVLFRASTRSLRRSS----------PILSKQFRGLVHETSGKFAPVQ--------- 60

Query: 61  FEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAKQG 120
                                VSL HHIVFNPF  QKFG+SS++ SHESSEN+EGSAKQ 
Sbjct: 61  ---------------------VSLLHHIVFNPFPFQKFGISSTT-SHESSENKEGSAKQS 120

Query: 121 NS-----DPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQP 180
            +     DP +DEN +VS+Q  ELNS  NSHST+SKSR   RRTIRTAFSDSDSE  EQP
Sbjct: 121 KNYSTTKDPTKDENSEVSDQAGELNSTSNSHSTKSKSRQWRRRTIRTAFSDSDSEGCEQP 180

Query: 181 SMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFA 240
           SMDDLVKSLA+KEELLK+KHKEI+ MQDKVLRSYAEMENV DRTRREAENSKKFAIQNFA
Sbjct: 181 SMDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFA 240

Query: 241 KSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGV 300
           KSLLDVADNLGRASSVVKDSFSKI ESEDS+GAVPLLKTLLEGVDMTEKQL++VFKKSGV
Sbjct: 241 KSLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGV 300

Query: 301 EKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVENS 360
           EKFDPT+EPFDPH+HNAVFQI DGSKP+GTVAIVLKSGYMLYDRVLRPAEVGVT+A E++
Sbjct: 301 EKFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVTRAAESN 324

BLAST of Spg017894 vs. ExPASy TrEMBL
Match: M5WTU3 (GrpE protein homolog OS=Prunus persica OX=3760 GN=PRUPE_4G223200 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 5.0e-90
Identity = 213/366 (58.20%), Postives = 254/366 (69.40%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSP-VQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIVCP 60
           M VSR+L RASR+L RS+  + +      PILC Q   L+HD                  
Sbjct: 1   MFVSRVLSRASRTLPRSTMLIAAPQNHHLPILCNQSQALIHD------------------ 60

Query: 61  FEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAKQG 120
                       F  K +   VSL HH   +  +SQ FG  SSSAS + SE E GSA + 
Sbjct: 61  ------------FSIKFSPRQVSLLHHSTPSSSISQIFGF-SSSASPQPSEREHGSAAEN 120

Query: 121 -----NSDPARDE-NLKVSEQVEELNSRLNSHSTESKSRWRSR-RTIRTAFSDSDSESDE 180
                N +PA++  + KVS+Q +E     +S S +S S  R R  T RTAFSDSDSE + 
Sbjct: 121 GGASTNGEPAKESGDAKVSDQTKESGYTSDSQSKKSPSVKRRRGGTKRTAFSDSDSEGEG 180

Query: 181 QPSMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQN 240
               DDLVK +A+KEELLK KH+EI+ MQDKVLRSYAEMENVMDRTRREAENSKKFA+QN
Sbjct: 181 DLGFDDLVKLVAEKEELLKQKHEEIKRMQDKVLRSYAEMENVMDRTRREAENSKKFAVQN 240

Query: 241 FAKSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKS 300
           FAKSLLDVADNLGRASSVVKDSFSK++ES++S GA PLLKTLLEGV+MTEKQL+EVF+K 
Sbjct: 241 FAKSLLDVADNLGRASSVVKDSFSKLDESKESGGAAPLLKTLLEGVEMTEKQLIEVFRKY 300

Query: 301 GVEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVE 359
           GVEKFDPTNE FDP+KHNAVF + D SKP GT+A+VLK GYMLYDRV+RPAEVGVT A E
Sbjct: 301 GVEKFDPTNEAFDPNKHNAVFNLEDASKPPGTIAVVLKPGYMLYDRVVRPAEVGVTTAPE 335

BLAST of Spg017894 vs. ExPASy TrEMBL
Match: A0A5E4FCQ4 (GrpE protein homolog OS=Prunus dulcis OX=3755 GN=ALMOND_2B019776 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 5.0e-90
Identity = 213/366 (58.20%), Postives = 254/366 (69.40%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSP-VQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIVCP 60
           M VSR+L RASR+L RS+  + +      PILC Q   L+HD                  
Sbjct: 1   MFVSRVLSRASRTLPRSTMLIAAPQNHHLPILCNQSQALIHD------------------ 60

Query: 61  FEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAKQG 120
                       F  K +   VSL HH   +  +SQ FG  SSSAS + SE E GSA + 
Sbjct: 61  ------------FSIKFSPRQVSLLHHSTPSSSISQIFGF-SSSASPQPSEREHGSAAEN 120

Query: 121 -----NSDPARDE-NLKVSEQVEELNSRLNSHSTESKSRWRSR-RTIRTAFSDSDSESDE 180
                N +PA++  + KVS+Q +E     +S S +S S  R R  T RTAFSDSDSE + 
Sbjct: 121 GGASTNGEPAKESGDAKVSDQTKESGYTSDSQSKKSPSVKRRRGGTKRTAFSDSDSEGEG 180

Query: 181 QPSMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQN 240
               DDLVK +A+KEELLK KH+EI+ MQDKVLRSYAEMENVMDRTRREAENSKKFA+QN
Sbjct: 181 DLGFDDLVKLVAEKEELLKQKHEEIKRMQDKVLRSYAEMENVMDRTRREAENSKKFAVQN 240

Query: 241 FAKSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKS 300
           FAKSLLDVADNLGRASSVVKDSFSK++ES++S GA PLLKTLLEGV+MTEKQL+EVF+K 
Sbjct: 241 FAKSLLDVADNLGRASSVVKDSFSKLDESKESGGAAPLLKTLLEGVEMTEKQLIEVFRKY 300

Query: 301 GVEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVE 359
           GVEKFDPTNE FDP+KHNAVF + D SKP GT+A+VLK GYMLYDRV+RPAEVGVT A E
Sbjct: 301 GVEKFDPTNEAFDPNKHNAVFNLEDASKPPGTIAVVLKPGYMLYDRVVRPAEVGVTTAPE 335

BLAST of Spg017894 vs. ExPASy TrEMBL
Match: A0A7J6G7J0 (GrpE protein homolog OS=Cannabis sativa OX=3483 GN=F8388_020706 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 1.5e-89
Identity = 216/359 (60.17%), Postives = 252/359 (70.19%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSS-PVQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIVCP 60
           M VSR+L R SR++ RSS  + S     FP L  QFH LV++ S                
Sbjct: 1   MFVSRVLARVSRTIPRSSLLLSSPQNHHFPNLSNQFHSLVNESS---------------- 60

Query: 61  FEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAKQG 120
                          K+    VSLF H+  +PF  Q+FG  +SSAS E+SE E+G+  + 
Sbjct: 61  --------------IKMAHGQVSLF-HLSSSPF--QRFGF-ASSASPETSEKEQGNNSEN 120

Query: 121 NSD-----PARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESDEQP 180
           N D      A      V +Q ++  S  +S ST S +    RRT RTAFSDSD  SDE  
Sbjct: 121 NGDAKVSSEAVSGQSDVDDQTKDSGSISDSQSTMSNNVKTRRRTKRTAFSDSD--SDEDL 180

Query: 181 SMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFA 240
           S+DDLVK +A+KEELLK KHKEIE MQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFA
Sbjct: 181 SIDDLVKLVAEKEELLKQKHKEIEKMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFA 240

Query: 241 KSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGV 300
           KSLLDVADNL RASSVVK+SFSKI+ S+DS+GA PLLKTLLEGV+MTEKQL+EVF+K G+
Sbjct: 241 KSLLDVADNLERASSVVKESFSKIDTSKDSSGATPLLKTLLEGVEMTEKQLLEVFRKYGL 300

Query: 301 EKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVEN 354
           EK+DPTNEPFDPH+HNAVFQ+ D SKP GTVA+VLKSGYMLYDRV+RPAEVGVTQ  EN
Sbjct: 301 EKYDPTNEPFDPHRHNAVFQLQDASKPPGTVAVVLKSGYMLYDRVIRPAEVGVTQEAEN 323

BLAST of Spg017894 vs. TAIR 10
Match: AT4G26780.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 282.7 bits (722), Expect = 4.1e-76
Identity = 184/360 (51.11%), Postives = 234/360 (65.00%), Query Frame = 0

Query: 1   MSVSRILFRASRSLQRSSPVQSQNL---QTFPILCRQFHGLVHDISGKVEVLKPCFQVIV 60
           M V RIL R +R+    S + +  L      P+   +FH L HD S K   L P      
Sbjct: 1   MLVLRILSRVTRNAGIRSSLSAVTLPARNQTPVFSSRFHSLAHDFSHK---LVPA----- 60

Query: 61  CPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFGVSSSSASHESSENEEGSAK 120
                                         + + F  Q+F  SSS+    S E++E   K
Sbjct: 61  ---------------------------QMSMMDSFALQRFNFSSST----SPESDE---K 120

Query: 121 QGNSDPARDENLKVSEQVEE--LNSRLNSHSTESKSRWR-SRRTIRTAFSDSDSES-DEQ 180
           + +++ ++    K + +  +  L+S      T+S  R R   +   ++ S+SDSES D++
Sbjct: 121 KTHTEASKTSEEKPTAEANQPGLDSESKDSVTDSAKRKRKGAKGAASSSSESDSESDDDE 180

Query: 181 PSMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNF 240
            S DDLVK +A+KEELL  K +EI+ ++DKVLR+YAEMENVMDRTRR+AEN+KK+A+QNF
Sbjct: 181 LSADDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAEMENVMDRTRRDAENTKKYAVQNF 240

Query: 241 AKSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSG 300
           AKSLLDVADNLGRASSVVK+SFSK++ SEDS GA PLLKTLLEGV+MTEKQL EVFKK G
Sbjct: 241 AKSLLDVADNLGRASSVVKESFSKLDTSEDSAGAAPLLKTLLEGVEMTEKQLAEVFKKFG 300

Query: 301 VEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVEN 354
           +EK+DP NEPFDP++HNAVFQ+PD SKPEGTVA VLKSGY LYDRV+RPAEVGVTQ  EN
Sbjct: 301 MEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRVIRPAEVGVTQGGEN 318

BLAST of Spg017894 vs. TAIR 10
Match: AT5G55200.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.8e-66
Identity = 170/358 (47.49%), Postives = 218/358 (60.89%), Query Frame = 0

Query: 1   MSVSRILFRASRS--LQRS-SPVQSQNLQTFPILCRQFHGLVHDISGKVEVLKPCFQVIV 60
           M VSR+L R SRS  L+ S S V +      PI+  +FH LVH    K         ++ 
Sbjct: 1   MLVSRVLSRVSRSAGLRSSFSSVVTPKRNQIPIVASRFHSLVHGTPNK---------LVA 60

Query: 61  CPFEAFCFACDCDIFGFKLTLFFVSLFHHIVFNPFLSQKFG-VSSSSASHESSENEEGSA 120
            P                     VSL +H   +  + Q+FG  SSSSA  + +E+     
Sbjct: 61  VP---------------------VSLRNHGTLDLNVLQRFGFFSSSSAEPKGNESNTEVP 120

Query: 121 KQGNSDPARDENLKVSEQVEELNSRLNSHSTESKSRWRSRRTIRTAFSDSDSESD-EQPS 180
           K G +     EN++V +                                +D+E D +  S
Sbjct: 121 KTGET----SENVEVGKA-------------------------------TDAEIDFDDLS 180

Query: 181 MDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAK 240
            DDLVK +++KE+LLK + K+I  M+DK LR+YAE +N+MDRT R AE++KKFA+QNFA 
Sbjct: 181 RDDLVKLVSEKEDLLKVQQKDIMEMKDKFLRTYAEQQNLMDRTNRNAESAKKFAVQNFAT 240

Query: 241 SLLDVADNLGRASSVVKDSFSKINESEDSNGAVPLLKTLLEGVDMTEKQLMEVFKKSGVE 300
           SLLDVADNL RASSVVK+SFSKI+ S+D  GA PLLK LLEGV+MTEKQL EVF+K+G+ 
Sbjct: 241 SLLDVADNLERASSVVKESFSKIDTSKDLAGATPLLKNLLEGVEMTEKQLAEVFRKAGLV 293

Query: 301 KFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLKSGYMLYDRVLRPAEVGVTQAVEN 354
           K DP NEPF+P++HNAVFQ+PD SKP+GT+A VLKSGY LYDRV+RPAEVGVT AVEN
Sbjct: 301 KEDPLNEPFNPNRHNAVFQVPDASKPKGTIAHVLKSGYSLYDRVIRPAEVGVTCAVEN 293

BLAST of Spg017894 vs. TAIR 10
Match: AT5G17710.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 49.3 bits (116), Expect = 7.5e-06
Identity = 61/258 (23.64%), Postives = 104/258 (40.31%), Query Frame = 0

Query: 91  PFLSQKFGVSSSSASHESSENEEGSAKQGNSDPARDENLKVSEQVEELNSRLNSHSTESK 150
           PF S +   + +       E  +G+    N + + +E     E+   + + L S+     
Sbjct: 63  PFASGEAETTETEVESNEPEETDGAVDVENENASAEEGEAEEEEAAVITALLKSYKEALA 122

Query: 151 SRWRSRRTIRTAFSDSDSESDEQPSMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSYAE 210
               +   I    +   S  DE+  + D V SL+           E+ + +D+++R  A+
Sbjct: 123 D--NNEGKIAEIEASLKSIEDEKFLLADKVASLS----------NELSVERDRLIRISAD 182

Query: 211 MENVMDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKINESEDSNGAVPL 270
            +N   RT RE  N    A     ++LL V DN  RA S +K       E E+       
Sbjct: 183 FDNFRKRTERERLNLVSNAQGEVVENLLAVLDNFERAKSQIKVE----TEGEEK------ 242

Query: 271 LKTLLEGVDMTEKQLMEVFKKSGVEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIVLK 330
              +        KQ +E+    GV   +   + FDP  H A+ +       EG V    +
Sbjct: 243 ---VTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEEYR 295

Query: 331 SGYMLYDRVLRPAEVGVT 349
            G++L +R+LRP+ V V+
Sbjct: 303 KGFLLGERLLRPSMVKVS 295

BLAST of Spg017894 vs. TAIR 10
Match: AT5G17710.2 (Co-chaperone GrpE family protein )

HSP 1 Score: 45.4 bits (106), Expect = 1.1e-04
Identity = 62/260 (23.85%), Postives = 105/260 (40.38%), Query Frame = 0

Query: 91  PFLSQKFGVSSSSASHESSENEE--GSAKQGNSDPARDENLKVSEQVEELNSRLNSHSTE 150
           PF S +   + +       E +E  G+    N + + +E     E+   + + L S+   
Sbjct: 63  PFASGEAETTETEVESNEPEVQETDGAVDVENENASAEEGEAEEEEAAVITALLKSYKEA 122

Query: 151 SKSRWRSRRTIRTAFSDSDSESDEQPSMDDLVKSLAQKEELLKAKHKEIEIMQDKVLRSY 210
                 +   I    +   S  DE+  + D V SL+           E+ + +D+++R  
Sbjct: 123 LAD--NNEGKIAEIEASLKSIEDEKFLLADKVASLS----------NELSVERDRLIRIS 182

Query: 211 AEMENVMDRTRREAENSKKFAIQNFAKSLLDVADNLGRASSVVKDSFSKINESEDSNGAV 270
           A+ +N   RT RE  N    A     ++LL V DN  RA S +K       E E+     
Sbjct: 183 ADFDNFRKRTERERLNLVSNAQGEVVENLLAVLDNFERAKSQIKVE----TEGEEK---- 242

Query: 271 PLLKTLLEGVDMTEKQLMEVFKKSGVEKFDPTNEPFDPHKHNAVFQIPDGSKPEGTVAIV 330
                +        KQ +E+    GV   +   + FDP  H A+ +       EG V   
Sbjct: 243 -----VTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMREDSAEYEEGIVLEE 297

Query: 331 LKSGYMLYDRVLRPAEVGVT 349
            + G++L +R+LRP+ V V+
Sbjct: 303 YRKGFLLGERLLRPSMVKVS 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023536001.11.7e-12976.26grpE protein homolog 2, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pep... [more]
KAG7024936.12.4e-12373.41grpE, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038898199.15.4e-12373.18grpE protein homolog 2, mitochondrial-like [Benincasa hispida][more]
XP_022936267.12.1e-12273.13grpE protein homolog 2, mitochondrial-like [Cucurbita moschata][more]
KAG6592058.13.5e-12272.85Transcriptional adapter ADA2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q8LB475.7e-7551.11GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE... [more]
Q9FLP35.4e-6547.49GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE... [more]
A4YJR16.2e-2944.03Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1[more]
Q3SW786.2e-2939.80Protein GrpE OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 10... [more]
B6JCI16.2e-2944.87Protein GrpE OS=Oligotropha carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC... [more]
Match NameE-valueIdentityDescription
A0A6J1F7Z21.0e-12273.13GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111442938 PE=3 SV=1[more]
A0A6J1CCY73.8e-12271.78GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111010351 PE=3 SV=1[more]
M5WTU35.0e-9058.20GrpE protein homolog OS=Prunus persica OX=3760 GN=PRUPE_4G223200 PE=3 SV=1[more]
A0A5E4FCQ45.0e-9058.20GrpE protein homolog OS=Prunus dulcis OX=3755 GN=ALMOND_2B019776 PE=3 SV=1[more]
A0A7J6G7J01.5e-8960.17GrpE protein homolog OS=Cannabis sativa OX=3483 GN=F8388_020706 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G26780.14.1e-7651.11Co-chaperone GrpE family protein [more]
AT5G55200.13.8e-6647.49Co-chaperone GrpE family protein [more]
AT5G17710.17.5e-0623.64Co-chaperone GrpE family protein [more]
AT5G17710.21.1e-0423.85Co-chaperone GrpE family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..203
NoneNo IPR availableCOILSCoilCoilcoord: 128..148
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 103..126
NoneNo IPR availablePANTHERPTHR21237:SF37GRPE PROTEIN HOMOLOGcoord: 88..354
IPR000740GrpE nucleotide exchange factorPRINTSPR00773GRPEPROTEINcoord: 205..221
score: 42.83
coord: 301..316
score: 46.29
coord: 328..347
score: 51.09
IPR000740GrpE nucleotide exchange factorPFAMPF01025GrpEcoord: 181..348
e-value: 3.5E-47
score: 160.1
IPR000740GrpE nucleotide exchange factorPANTHERPTHR21237GRPE PROTEINcoord: 88..354
IPR000740GrpE nucleotide exchange factorPROSITEPS01071GRPEcoord: 304..347
IPR000740GrpE nucleotide exchange factorHAMAPMF_01151GrpEcoord: 110..349
score: 18.405445
IPR000740GrpE nucleotide exchange factorCDDcd00446GrpEcoord: 200..347
e-value: 2.72554E-58
score: 183.476
IPR013805GrpE nucleotide exchange factor, coiled-coilGENE3D3.90.20.20coord: 175..291
e-value: 1.0E-27
score: 98.0
IPR013805GrpE nucleotide exchange factor, coiled-coilSUPERFAMILY58014Coiled-coil domain of nucleotide exchange factor GrpEcoord: 184..293
IPR009012GrpE nucleotide exchange factor, headGENE3D2.30.22.10Head domain of nucleotide exchange factor GrpEcoord: 293..348
e-value: 3.0E-20
score: 73.9
IPR009012GrpE nucleotide exchange factor, headSUPERFAMILY51064Head domain of nucleotide exchange factor GrpEcoord: 294..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017894.1Spg017894.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0001405 PAM complex, Tim23 associated import motor
molecular_function GO:0000774 adenyl-nucleotide exchange factor activity
molecular_function GO:0051087 chaperone binding
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0051082 unfolded protein binding