Spg017554 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017554
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSubtilisin-like protease SBT2.5
Locationscaffold4: 40932639 .. 40939136 (+)
RNA-Seq ExpressionSpg017554
SyntenySpg017554
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCGTACTGTTAACCAACAAGCCGTCGCCGTTGTCCGTGGACATCGCCGCCTACGAGAGAGGATTCATGGAGGGGGTTTCCAAACTCTAAATTCTTTGGAACTATGCTTCTTCTTTTCAACATTTTCAATCCCAAACGAGTTCGTTCTCTAACTTCGTCTTCCAACGATTCCTCGACAAGCCGATGTCTTCAAACCTTGTTAAAAAGCGGCTTCTCTCCTACTCTGAAATCCATCAATCACTTCCTTCGTTTTCTCTACAACAACCACAGATTCAACTATGTCATCAATTTCTTCTCCCAAATAAAGGCGAACCAATTCAAAGGAAACTCTCGAACTCACTTGATTCTCTCATGGGCTCTCCTCAAATCCCATAAATATGACGAATTAGAGCAAATCTTGAACACCCAGATGGAGATGGCTTCAAGTTTTCATCGAAATCGCATGTGGAACTTGCTCATTCGAGGGCTTTGTGTCAACCAAGAAGACCCAGAAAAGGCATTGTGGGTTTTGCGGGATTGCTTGAGAAATCATGGTATTTTGCCTTCTTCTTTCACTTTCTGTGTTTTGATTCATAAGTTTAGTTCTCTTGGAATGATGGATAAGACAGTTGAGATTCTAGAGCTAATGTCTGATGAAAATGTGAACTACCCATTTGATAATTTTGTCTGTAGTTCTGTAATTTCCGGGTTTTGTAACATTGGGAAACCGGAATTGGCTCTGAAGTTTTTCGAAAATGCCACAACATTGGGGAACTTGAAACCTAATTTGGTGACTTATACTGCTCTGACCAGTGCACTCTGTAAATTACATAAAGTTAATGAAGTTTGGGATTTGCTTTGTAAAATGGAGAAGGAGAATTTGTCATTGGACGTTGTTTTCTATAGTTGCTGGATTTGTGGGTATATTTCCGAGAGCTTGTTATTAGACGTGTTCAAGAGGAACAAGGAAATGGTTCAGAAGGGAATTAGACCTGATACAATAAGTTATACGGTTCTGATACATGGTCTTTCCAAGTTGGGAGATGTAGAGAAGGCATTTGGGGTTTTAGAGAGGATGAAGAAATCAGGATTAGATCCAAGTTCAGTTACGTATACGGTGATTATGTTGGGATTTTGCAAGAAAGGGAAAGTGGAGGAAGCATTTGCTCTTTTCGAAATGGTTAAGGGTTCGGGGATGGAGGTGGATGAATTTATGTATGCAACTCTGATTGATGGATGTAGCAGAAAAGGAGATTTTGATCGTGTTTTTGGTCTTCTTGATGAAATGGAAACTAGAGGGATGAAACCAAGTATTGTTACATACAATACTGTGATTAATGGACTCTGTAAATTGGGGAGAACATCTGAGGCAGATAGACTATCAAAGGGCTTACATGGAGATGTTATTACATATAGTACACTCTTACATGGATATGTCCAAGAACAGAACATCACTGGAATTTTCGAAACAAAGACGAGACTTGAAGATGCCGGGATTTCTCTGGATGTTGTCATGTGTAATGTTTTGATCAAAGCATTGTTTCTGGTTGGGGCATTTGAAGATGCTTATATACTCTACAAGAGAATGCCAGAAATGGGTCTTGTTGCAAATTCAGTTACCTACCATACAATGATCAATGGATATTGTAATATTAGTAGGATTGATGAAGCAATTGAGATATTCAATGAGTTCAAGTCTGCCTCATGTGATTCAGTGGCCGTTTACAATAGTATCATGAAAGCACTTTGCAGAGGAGGTCTAGTAGAAAAGGCCTACGAGATATTTATTGAACTGAACCTCAAAGGTTTGACTTTGGATGTAGGTGTGTATAAGATGATTATCAAAACCATTTTTGAAGAAAAAGGTGCAGCAGGGCTTTGTGAAGCACTGTATGGGATGGAAAAATTGGAACAAGATGTATACAATTTTACATGCAATTATGCTATTCAGTTCCTATGCAAGAGAGGTTTCCCGGAGATGGCGAGTGAATTGTATTCAAGAATGAGGAGAATCGGTTCGCTTCTTGAAAACAAAACATATTATTTGCTTGTAAAAGCATTGAATAGTGAAGGAAAGACATGGATAAGTCGGTCTATTCTCTGTAACTTTTTGAAAGAATTTGGCCTTGCTGAGCCCATCGTTAAGCAGATAATTGCAGACTTTCAATGCACAAAGTTCACTCATTCAACTTCAGAGAAAATGAAAGAGAAATTTTCAACATTTATGGTACCTGATACCATGTTTAAAATGCTAGTAAGAGAAGGAAGATTTTCTGATGCTTATAATCTTGTGATGAAGAGTGGAAACAATCTCTTGCTTGGGGACGTATATGATTATTCAATTCTGATTCATGGTCTTTGTAAAGGTGGGCAAATGATTGAAGCGCTGGATATTTGTGTTTTTGCCAAAGCAAATGGAATAAAGTTGAATATTGTGTCTTATAATATAGTCATAAAGGGACTGTGCCTCCAAAGTCGTCTTACCGAAGCATTTCGGCTCTTTGATTCATTGGAAAGAATAGGCTTGATACCTACTGAAATCACATATGGAACTCTAATTGACTCTTTATGCAGAGAAGGATACTTGGAAGATGCGAGGCAGTTGTTTGAAAGGATGATCTCGAATGGCCTTAAACCAAATACCCATATTTACAATTCTTTAATTGATGGTTACCTCAGGATTGGTCAAATTGAAGAAGCCTTTAAGCTTTTAAATGAATTGGGGACGGAAGTCTTTAATCCTGATGAATTATCCGTGAGCTCTGCTATCAAGGCTTATTGTCGAAAGGGCGACATGGAAGGGGCTCTTTCATTCTATTTTGACTTCAGGACTAAAGGCATTTTACCTGATTTCTTGGGCTTCTTATATTTGATAAGAGGTCTCTGTGCCAAGGGAAGGATGGAAGAAGCTAGGGATATCATACGCGAGATGATACAGTCACAATCAGTGATGGAGTTGATTAACAAGGTTGATTCTGAGATCGAAACTGAGTCTATAGGAAACACCCTCACTCATTTATGCGAGGAAGGACGTATCTTAGAAGCCTACGTCGTTCTTAATGAAGTTGGCTCTATATTTTTCTCTCCTCAGAGGCATTCTACTGATTACAATCAACCTCATAAACTGCATATAAATGACAAAAAATCTGTAAATATTGTTCGTTCTGGACCTGAAGCCTACTCGTGCATTACTCTTCCAAGTTTCAGAGCCTCAGATGTTTACACAATTGAAGATGCAGAGTATGAGAATCTGGAAAAGAGGCCTCATTTTGAGGACTTCAATTGTTACTACCCTCTACTTTCTTCCCTTTGTTCAGAAGGAAATGTTCAAAAGGCTACTCAATTAGCGAAAGAAGTAATTTCCAATTTGGATAGAGGCTAACTGTAAAGTAGAAAATAGAAGAACTCGGCTTCTTCGGTAATCGGAGTTCCTTTCCATTAATGTGAGGGATAAAGAAAAAGATTTTTTTTCCCCATTGCCAGATGCTGATCAATGGGCAAAGGCTCTTTAACCTGGTTGGAGGCTGATTGCTTAACAGAAACGGTCTCGAGTATTTCACTTCAAGGAGTTCCCTCTATGAATGTGACAAGTGTAGAATTAGTCTGCACAGGTACACATGATTTGTTATTACATTATAGATGCAAGCATTGGCTGCATTTTTGTGTCATTCTCTTTGATTGCCTTCTTGAAATATGAACTGTTGAGGAAATGTATGTTTTACATGTAGCCCCTTTAATGGGTTAACTTTTGTGGGCTTGGTTTTTTGTATGCCTGTGTATTCTTTCATTTCTTCTCAATGAAAGTTGGTTCTTTATAAAAAAAAAAAATATTTTACATGTAAATGCAAACCTATGTGTATAATTTTTAATCTTGAAAAGTTCTCATGGAAACTTTCTTCTCAATGAAAGTTGGTTCTGTTGCATGCTAGTGCTGGAAAATATAATATTTATGAATAATTACATATTTCATGTACTATGGTTAATTCTTTTATACCCTCATAAATTATTACCTCCTCCTCCCAACAAAAAGCTTATCATCTTAAAGTGTATCTTTGCTCTTCGAAAATAAGGTTTTGTCTGGATGTGCATATTATAATATCCTTAGTACAGAACACTTGAGCTACTCTTGATATTTGGATTTTCCCTTTCGCCATCTTTTGCCTTTCTTTTCCATATATTTTCTTTATGTTTGCTTGTTCCTTCTCAGCTACTGTCTCACTTACTATTTGGAGATTTTGTACTTTGCAATTTACTTTTAGGATCTTCTAGTCTAGACTACTACAACACCTCTACACCCAGAGCTGGACGGGTCGGGTCAAGAGCTTTGATGCTGTGGTGGGTAGCGTGGAGATGGTTTGATGCCTTTATTTGACAAATTAGCTCCTGAGGTAGCATTGTTTTCTGCTCGTGTGCCCAATATTAGAGATTTTAGCTTTCAGGATGCAGACCTTCTCAAACCAGATATTCTAGCTCCTGGTTCTTTCATTTGGGCTGCTTGGTCATCAAATGGAACGGACAAGCCGAACTATGTTGGTTTGCATTATTGAATCCATTTATCTTATTACTAATGTTCCTTTTAGAGCCACCACCATGCTTATATCAGTTCAAACCACGTGAATGGTATCCCTTCCAAAATAAATGAACAAAAACAGAGAAAAAAACGGAGAAACAAAGTTAAATGGTTAATAGAGTTCGATGTAATTTTTGCTTCATCTTTTTGGAACAAAGGATCCCAAAAAGTAGATAACTGTTTTAATGCTTTCTCATATTTTGGTTTTATTATTTAAAATGAAATACTCTATAGTTTCCTTTATCGATTCCAGAAGTGGAGGTAGCTCTAACTGTTTACTTATTCCAGGAGAGAGATTTGCGATGATTTCTGGGACTAGCATGGCAGCACCACATATAGCTGGTGCAACAGCTCTTGTAAAACAGAAGCATCCTAACTGGAGTCCAGCAGCCATCAAATCAGCTTTGATGACGCTGACAACAACGGACAGAGCAGGAAGACTCTTAAAGCACAACAGTTTTCTGAAACAGAAGCCATGAAACTGGTAACTGCAACACCTTTTGATCATTGGAGTGGTCTTGTAAACCCAAGAGCGGCACTGGATCCTGGACTCATCTCTGATGCAGGTTCATTTTGCTTTGCCTATCTAAATTTGATATTTTTTTTTTTTTTTTGAAATGCTTTGCCTATCTAAATTTGATATTTGAACCATACATTTGATGATGAACTAATTGATCATGTCAGTTAAATTTGAACATGGATCTCTATCAGATCGAGACTGATAGGAATGTCATTAGAAGAAAATTAGGAATATTAGTAAGGGTATACTAGCAATTAGTAAGAAGTTTGTTAAAGTATTTGGTGATAAATAGAGGGAGTGGGAAACAAGAAAGTTGGGCATTATTTTGGTTGGTGAATTAGGGGCTTGAGAGAGATCTCGGTTACTTGTTTATCTTGTAAGTTCCTTACCTTTTATATTGCAATACATTGTGGTTTGTTTTGGTATGGGAAGCCACTAGAAGGGAATACATCTTAATCCATCATTTCTTTCGGATAATCTAAGAATTGGTTCATGGATTGTGCTCACTTTTCTTTCTTTGTTCTTTTCTCTGGTAAAACATTAACTAAACTTAGCAACCCTATTAAGAAAAAAACAAATCAAATATTCAATTCTCAAAAGATTGAGAATTATATGTCAACATTCTGACTTGAGAAATTGAGATGTAAATGAGGCAAAATAACAATGAGCATGATGGGAACAAAAAGTTCCATATGAGATTAAAAAGTGACATGTCTTTTTTTCCACTGAATTTTTCTAGGATGCTGATGGCACTTAAATGTCAGTTTCCATGTCTGCACTGCATATCTGCATTCAGACATACCTTTTCCTTAATCATGTACTGCAGTTGAGTAGTTTTTCGAATATGTATGCAATGGCTCACGTCGAATCTTGCTAGATCTGTGCATCAACGTTCACAAGATACACAAATATAGCAGCCCATCTTGCAACTTCAGCATGGGCCATTCCTGGAATCTCGACAACCCATCAAGCACCATCGTCCATCTTGTGGGAACTCAAGACTGTTACTAGCACAGTAACAAATGTTGCTGAGGCAGACACCTCTACAATCACTGCAAGAATGGACCCTGCTGTTGCCATAGAAACAAATCCTCCAGCAATGGCCTTACAATCCAGTTCGTCGAGAAAATTATCTCTCAGCTACTCTCGCAGCTCGATCCCTGACAGGAACATATAGTTTTGGTAAGGTTCTTTGTTTAGCTTTTGCATTCTGAGCTTTCTTCTCCCTTTTCAGATTCCTCATTCTCTGTTGGCTTGTTGCAATAGTTGGGATCGTGGTTTTTGAAATGGATCCTTGAATGCAATCAATAGCCATTTTGAATCTGGTCTCTAAATATAAATATGAATCGATTTTTTCGTATCATACCAACCCACACACGTACTACAAATGGACTTAGTGATGAGCAAGTACTGATGGATCATTAGAAAA

mRNA sequence

ATGGGCAAAGGCTCTTTAACCTGGTTGGAGGCTGATTGCTTAACAGAAACGGTCTCGAGTATTTCACTTCAAGGAGTTCCCTCTATGAATGTGACAAGTGTAGAATTAGTCTGCACAGCTCCTGAGGTAGCATTGTTTTCTGCTCGTGTGCCCAATATTAGAGATTTTAGCTTTCAGGATGCAGACCTTCTCAAACCAGATATTCTAGCTCCTGGAGAGAGATTTGCGATGATTTCTGGGACTAGCATGGCAGCACCACATATAGCTGGTGCAACAGCTCTTAGCAGGAAGACTCTTAAAGCACAACAGTTTTCTGAAACAGAAGCCATGAAACTGGTAACTGCAACACCTTTTGATCATTGGAGTGGTCTTGTAAACCCAAGAGCGGCACTGGATCCTGGACTCATCTCTGATGCAGTTTCCATGTCTGCACTGCATATCTGCATTCAGACATACCTTTTCCTTAATCATATCTGTGCATCAACGTTCACAAGATACACAAATATAGCAGCCCATCTTGCAACTTCAGCATGGGCCATTCCTGGAATCTCGACAACCCATCAAGCACCATCGTCCATCTTGTGGGAACTCAAGACTGTTACTAGCACAGTAACAAATGTTGCTGAGGCAGACACCTCTACAATCACTGCAAGAATGGACCCTGCTGTTGCCATAGAAACAAATCCTCCAGCAATGGCCTTACAATCCAGTTCGTCGAGAAAATTATCTCTCAGCTACTCTCGCAGCTCGATCCCTGACAGGAACATATAG

Coding sequence (CDS)

ATGGGCAAAGGCTCTTTAACCTGGTTGGAGGCTGATTGCTTAACAGAAACGGTCTCGAGTATTTCACTTCAAGGAGTTCCCTCTATGAATGTGACAAGTGTAGAATTAGTCTGCACAGCTCCTGAGGTAGCATTGTTTTCTGCTCGTGTGCCCAATATTAGAGATTTTAGCTTTCAGGATGCAGACCTTCTCAAACCAGATATTCTAGCTCCTGGAGAGAGATTTGCGATGATTTCTGGGACTAGCATGGCAGCACCACATATAGCTGGTGCAACAGCTCTTAGCAGGAAGACTCTTAAAGCACAACAGTTTTCTGAAACAGAAGCCATGAAACTGGTAACTGCAACACCTTTTGATCATTGGAGTGGTCTTGTAAACCCAAGAGCGGCACTGGATCCTGGACTCATCTCTGATGCAGTTTCCATGTCTGCACTGCATATCTGCATTCAGACATACCTTTTCCTTAATCATATCTGTGCATCAACGTTCACAAGATACACAAATATAGCAGCCCATCTTGCAACTTCAGCATGGGCCATTCCTGGAATCTCGACAACCCATCAAGCACCATCGTCCATCTTGTGGGAACTCAAGACTGTTACTAGCACAGTAACAAATGTTGCTGAGGCAGACACCTCTACAATCACTGCAAGAATGGACCCTGCTGTTGCCATAGAAACAAATCCTCCAGCAATGGCCTTACAATCCAGTTCGTCGAGAAAATTATCTCTCAGCTACTCTCGCAGCTCGATCCCTGACAGGAACATATAG

Protein sequence

MGKGSLTWLEADCLTETVSSISLQGVPSMNVTSVELVCTAPEVALFSARVPNIRDFSFQDADLLKPDILAPGERFAMISGTSMAAPHIAGATALSRKTLKAQQFSETEAMKLVTATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAHLATSAWAIPGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNPPAMALQSSSSRKLSLSYSRSSIPDRNI
Homology
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BLAST of Spg017554 vs. NCBI nr
Match: XP_022996483.1 (subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima] >XP_022996484.1 subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 193.4 bits (490), Expect = 2.5e-45
Identity = 136/262 (51.91%), Postives = 156/262 (59.54%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG +AL +                          + LKAQQFSETEAMKLV
Sbjct: 596 GTSMAAPHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 656 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 715

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 252
                 W +  P I+  H      L   KTVT TVTNVAE +T TITARMDPAVAIET+P
Sbjct: 716 FTMGHPWNLNTPSITIAH------LVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSP 775

[Close]
BLAST of Spg017554 vs. NCBI nr
Match: XP_022996485.1 (subtilisin-like protease SBT2.5 isoform X2 [Cucurbita maxima])

HSP 1 Score: 193.4 bits (490), Expect = 2.5e-45
Identity = 136/262 (51.91%), Postives = 156/262 (59.54%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 416 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMIS 475

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG +AL +                          + LKAQQFSETEAMKLV
Sbjct: 476 GTSMAAPHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV 535

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 536 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 595

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 252
                 W +  P I+  H      L   KTVT TVTNVAE +T TITARMDPAVAIET+P
Sbjct: 596 FTMGHPWNLNTPSITIAH------LVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSP 655

[Close]
BLAST of Spg017554 vs. NCBI nr
Match: XP_022966056.1 (subtilisin-like protease SBT2.5 [Cucurbita maxima] >XP_022966057.1 subtilisin-like protease SBT2.5 [Cucurbita maxima])

HSP 1 Score: 192.2 bits (487), Expect = 5.5e-45
Identity = 134/261 (51.34%), Postives = 153/261 (58.62%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEAFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + LKAQQFSETE MKLV
Sbjct: 596 GTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEVMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 656 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 715

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 251
                 W +  P I+  H      L   KTVT TVTNVAE +T T+TARMDPA+AIETNP
Sbjct: 716 FTMGHPWNLNSPSITIAH------LVGTKTVTRTVTNVAEEETYTVTARMDPAIAIETNP 775

[Close]
BLAST of Spg017554 vs. NCBI nr
Match: KAG6598892.1 (Subtilisin-like protease 2.5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029843.1 Subtilisin-like protease SBT2.5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 192.2 bits (487), Expect = 5.5e-45
Identity = 132/261 (50.57%), Postives = 151/261 (57.85%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG +AL +                          + LKAQQFSETEAMKLV
Sbjct: 596 GTSMAAPHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAHL 218
           +ATPFD+ SG VNPRAALDPGLI DA              +L  +C +       I  H 
Sbjct: 656 SATPFDYGSGHVNPRAALDPGLIFDAGYED----------YLGFLCTTAGINVDEIHNHT 715

Query: 219 ATSAWAIPGISTTHQAPSSILWEL---KTVTSTVTNVAEADTSTITARMDPAVAIETNPP 252
            +      G       PS  +  L   KTVT TVTNVAE +T TITARMDPA+AIET+PP
Sbjct: 716 NSPCNFTMGHPWNLNTPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAIAIETSPP 775

[Close]
BLAST of Spg017554 vs. NCBI nr
Match: XP_022937793.1 (subtilisin-like protease SBT2.5 [Cucurbita moschata] >XP_022937794.1 subtilisin-like protease SBT2.5 [Cucurbita moschata])

HSP 1 Score: 192.2 bits (487), Expect = 5.5e-45
Identity = 134/261 (51.34%), Postives = 153/261 (58.62%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEAFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + LKAQQFSETE MKLV
Sbjct: 596 GTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEVMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 656 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 715

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 251
                 W +  P I+  H      L   KTVT TVTNVAE +T T+TARMDPA+AIETNP
Sbjct: 716 FTMGHPWNLNSPSITIAH------LVGTKTVTRTVTNVAEEETYTVTARMDPAIAIETNP 775

[Close]
BLAST of Spg017554 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 8.0e-39
Identity = 119/262 (45.42%), Postives = 145/262 (55.34%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +AP+VALFSAR PN +DFSFQDADLLKPDILAP                   GE FA+IS
Sbjct: 534 SAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALIS 593

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + L+AQQ+S+TEA+ LV
Sbjct: 594 GTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLV 653

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAHL 218
            ATPFD+ SG VNP AALDPGLI DA     L     T      I A     YTN A + 
Sbjct: 654 KATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTT----PGISAHEIRNYTNTACNY 713

Query: 219 ---ATSAWAIPGISTTHQAPSSILWELKTVTSTVTNVAEA-DTSTITARMDPAVAIETNP 252
                S +  P I+ +H      L   +TVT  VTNVAE  +T TITARM P++AIE NP
Sbjct: 714 DMKHPSNFNAPSIAVSH------LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNP 773

[Close]
BLAST of Spg017554 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.2e-37
Identity = 116/262 (44.27%), Postives = 146/262 (55.73%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PN +DFSFQDADLLKPDILAP                   GE FA+IS
Sbjct: 535 SAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALIS 594

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + L+AQQ+SETE + LV
Sbjct: 595 GTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLV 654

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAH- 218
            ATPFD+ SG VNP AALDPGLI DA     +     T      I A     +TN   + 
Sbjct: 655 KATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTT----PGIDAHEIKNFTNTPCNF 714

Query: 219 --LATSAWAIPGISTTHQAPSSILWELKTVTSTVTNVA-EADTSTITARMDPAVAIETNP 252
             +  S +  P I+ +H      L   +TVT  VTNVA E +T TIT+RM+PA+AIE +P
Sbjct: 715 KMVHPSNFNTPSIAISH------LVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSP 774

[Close]
BLAST of Spg017554 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.6e-16
Identity = 56/144 (38.89%), Postives = 72/144 (50.00%), Query Frame = 0 

Query: 40  APEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMISG 99
           AP+V  +SAR P+  D SF DAD+LKP+++AP                   GE+FAM+SG
Sbjct: 558 APKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTEFEGEKFAMMSG 617

Query: 100 TSMAAPHIAGATALSRKT--------------------------LKAQQFSETEAMKLVT 139
           TSMAAPH+AG  AL +++                          + AQ+        L T
Sbjct: 618 TSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKGSPIMAQRTYSNPDQSLYT 677

[Close]
BLAST of Spg017554 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.8e-14
Identity = 74/250 (29.60%), Postives = 109/250 (43.60%), Query Frame = 0 

Query: 40  APEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMISG 99
           AP++  +SAR P+ +D  F DAD+LKP+++AP                   GE FAM+SG
Sbjct: 573 APKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSG 632

Query: 100 TSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLVT 159
           TSMAAPH+AG  AL +                          + + AQ+        +  
Sbjct: 633 TSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISP 692

Query: 160 ATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAHLA 219
           ATPFD  +G VN  AALDPGLI D      +            +   T T      A ++
Sbjct: 693 ATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAPVVFNYTGTNCLRNNATIS 752

Query: 220 TSAWAIPGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNPPAMAL 245
            S   +P I+       S L   +TV   +TN+A  +T T++      V I  +P   ++
Sbjct: 753 GSDLNLPSITV------SKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSI 812

[Close]
BLAST of Spg017554 vs. ExPASy Swiss-Prot
Match: Q9SA75 (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.8e-13
Identity = 84/262 (32.06%), Postives = 118/262 (45.04%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           TAP+V  FSAR P+  D SF DAD++KP+++AP                   GERFAM S
Sbjct: 547 TAPKVMYFSARGPDPEDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMES 606

Query: 99  GTSMAAPHIAGATALSRKT--------------------------LKAQQFSETEAMKLV 158
           GTSM+APH+ G  AL ++                           + AQ+      +   
Sbjct: 607 GTSMSAPHVTGIAALIKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQS 666

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYL-FLNHICAST--FTRYTNIA 218
            ATPFD  SG VN  AALDPGLI D        I    Y+ FL  I  S+     YT  +
Sbjct: 667 PATPFDMGSGFVNATAALDPGLIFD--------IGYNEYMKFLCGINGSSPVVLNYTGES 726

Query: 219 AHLATSAWAIPGISTTHQAPSSILWEL---KTVTSTVTNVAEADTST--ITARMDP-AVA 247
                S+ A   ++     PS  + +L   + V   VTN+A   T+   I   M P +V+
Sbjct: 727 CSSYNSSLAASDLN----LPSVTIAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVS 786

[Close]
BLAST of Spg017554 vs. ExPASy TrEMBL
Match: A0A6J1KAV8 (subtilisin-like protease SBT2.5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491718 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-45
Identity = 136/262 (51.91%), Postives = 156/262 (59.54%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG +AL +                          + LKAQQFSETEAMKLV
Sbjct: 596 GTSMAAPHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 656 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 715

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 252
                 W +  P I+  H      L   KTVT TVTNVAE +T TITARMDPAVAIET+P
Sbjct: 716 FTMGHPWNLNTPSITIAH------LVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSP 775

[Close]
BLAST of Spg017554 vs. ExPASy TrEMBL
Match: A0A6J1K6W7 (subtilisin-like protease SBT2.5 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491718 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-45
Identity = 136/262 (51.91%), Postives = 156/262 (59.54%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 416 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMIS 475

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG +AL +                          + LKAQQFSETEAMKLV
Sbjct: 476 GTSMAAPHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV 535

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 536 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 595

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 252
                 W +  P I+  H      L   KTVT TVTNVAE +T TITARMDPAVAIET+P
Sbjct: 596 FTMGHPWNLNTPSITIAH------LVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSP 655

[Close]
BLAST of Spg017554 vs. ExPASy TrEMBL
Match: A0A6J1HNB4 (subtilisin-like protease SBT2.5 OS=Cucurbita maxima OX=3661 GN=LOC111465779 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.7e-45
Identity = 134/261 (51.34%), Postives = 153/261 (58.62%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEAFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + LKAQQFSETE MKLV
Sbjct: 596 GTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEVMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 656 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 715

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 251
                 W +  P I+  H      L   KTVT TVTNVAE +T T+TARMDPA+AIETNP
Sbjct: 716 FTMGHPWNLNSPSITIAH------LVGTKTVTRTVTNVAEEETYTVTARMDPAIAIETNP 775

[Close]
BLAST of Spg017554 vs. ExPASy TrEMBL
Match: A0A1S3BRZ6 (subtilisin-like protease SBT2.5 OS=Cucumis melo OX=3656 GN=LOC103492606 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.7e-45
Identity = 136/262 (51.91%), Postives = 153/262 (58.40%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + LKAQQFSETEAMKLV
Sbjct: 596 GTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           TATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 656 TATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 715

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 252
                 W +  P I+  H      L   K VT TVTNVAE +T TITARMDPAVAIE NP
Sbjct: 716 FTMGHPWNLNSPSITIAH------LVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNP 775

[Close]
BLAST of Spg017554 vs. ExPASy TrEMBL
Match: A0A6J1FC86 (subtilisin-like protease SBT2.5 OS=Cucurbita moschata OX=3662 GN=LOC111444081 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.7e-45
Identity = 134/261 (51.34%), Postives = 153/261 (58.62%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PNIRDFSFQDADLLKPDILAP                   GE FAMIS
Sbjct: 536 SAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEAFAMIS 595

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + LKAQQFSETE MKLV
Sbjct: 596 GTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEVMKLV 655

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSAL-HICIQTYLFLNHICASTFTRYTNIAAH 218
           +ATPFD+ SG VNPRAALDPGLI DA     L  +C    + ++ I       YTN   +
Sbjct: 656 SATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEI-----HNYTNSPCN 715

Query: 219 LATS-AWAI--PGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNP 251
                 W +  P I+  H      L   KTVT TVTNVAE +T T+TARMDPA+AIETNP
Sbjct: 716 FTMGHPWNLNSPSITIAH------LVGTKTVTRTVTNVAEEETYTVTARMDPAIAIETNP 775

[Close]
BLAST of Spg017554 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 162.2 bits (409), Expect = 5.7e-40
Identity = 119/262 (45.42%), Postives = 145/262 (55.34%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +AP+VALFSAR PN +DFSFQDADLLKPDILAP                   GE FA+IS
Sbjct: 534 SAPQVALFSARGPNTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALIS 593

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + L+AQQ+S+TEA+ LV
Sbjct: 594 GTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLV 653

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAHL 218
            ATPFD+ SG VNP AALDPGLI DA     L     T      I A     YTN A + 
Sbjct: 654 KATPFDYGSGHVNPSAALDPGLIFDAGYEDYLGFLCTT----PGISAHEIRNYTNTACNY 713

Query: 219 ---ATSAWAIPGISTTHQAPSSILWELKTVTSTVTNVAEA-DTSTITARMDPAVAIETNP 252
                S +  P I+ +H      L   +TVT  VTNVAE  +T TITARM P++AIE NP
Sbjct: 714 DMKHPSNFNAPSIAVSH------LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNP 773

[Close]
BLAST of Spg017554 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 158.3 bits (399), Expect = 8.2e-39
Identity = 116/262 (44.27%), Postives = 146/262 (55.73%), Query Frame = 0 

Query: 39  TAPEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMIS 98
           +APEVALFSAR PN +DFSFQDADLLKPDILAP                   GE FA+IS
Sbjct: 535 SAPEVALFSARGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALIS 594

Query: 99  GTSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLV 158
           GTSMAAPHIAG  AL +                          + L+AQQ+SETE + LV
Sbjct: 595 GTSMAAPHIAGIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLV 654

Query: 159 TATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAH- 218
            ATPFD+ SG VNP AALDPGLI DA     +     T      I A     +TN   + 
Sbjct: 655 KATPFDYGSGHVNPSAALDPGLIFDAGYEDYIGFLCTT----PGIDAHEIKNFTNTPCNF 714

Query: 219 --LATSAWAIPGISTTHQAPSSILWELKTVTSTVTNVA-EADTSTITARMDPAVAIETNP 252
             +  S +  P I+ +H      L   +TVT  VTNVA E +T TIT+RM+PA+AIE +P
Sbjct: 715 KMVHPSNFNTPSIAISH------LVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSP 774

[Close]
BLAST of Spg017554 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 86.3 bits (212), Expect = 4.0e-17
Identity = 56/144 (38.89%), Postives = 72/144 (50.00%), Query Frame = 0 

Query: 40  APEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMISG 99
           AP+V  +SAR P+  D SF DAD+LKP+++AP                   GE+FAM+SG
Sbjct: 558 APKVMYYSARGPDPEDNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTEFEGEKFAMMSG 617

Query: 100 TSMAAPHIAGATALSRKT--------------------------LKAQQFSETEAMKLVT 139
           TSMAAPH+AG  AL +++                          + AQ+        L T
Sbjct: 618 TSMAAPHVAGVAALIKQSYPQFTPSTISSALSTTALLNDNKGSPIMAQRTYSNPDQSLYT 677

[Close]
BLAST of Spg017554 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 79.3 bits (194), Expect = 4.9e-15
Identity = 74/250 (29.60%), Postives = 109/250 (43.60%), Query Frame = 0 

Query: 40  APEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMISG 99
           AP++  +SAR P+ +D  F DAD+LKP+++AP                   GE FAM+SG
Sbjct: 573 APKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSG 632

Query: 100 TSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLVT 159
           TSMAAPH+AG  AL +                          + + AQ+        +  
Sbjct: 633 TSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISP 692

Query: 160 ATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAHLA 219
           ATPFD  +G VN  AALDPGLI D      +            +   T T      A ++
Sbjct: 693 ATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAPVVFNYTGTNCLRNNATIS 752

Query: 220 TSAWAIPGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNPPAMAL 245
            S   +P I+       S L   +TV   +TN+A  +T T++      V I  +P   ++
Sbjct: 753 GSDLNLPSITV------SKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSI 812

[Close]
BLAST of Spg017554 vs. TAIR 10
Match: AT4G20430.2 (Subtilase family protein )

HSP 1 Score: 79.3 bits (194), Expect = 4.9e-15
Identity = 74/250 (29.60%), Postives = 109/250 (43.60%), Query Frame = 0 

Query: 40  APEVALFSARVPNIRDFSFQDADLLKPDILAP-------------------GERFAMISG 99
           AP++  +SAR P+ +D  F DAD+LKP+++AP                   GE FAM+SG
Sbjct: 549 APKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSG 608

Query: 100 TSMAAPHIAGATALSR--------------------------KTLKAQQFSETEAMKLVT 159
           TSMAAPH+AG  AL +                          + + AQ+        +  
Sbjct: 609 TSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISP 668

Query: 160 ATPFDHWSGLVNPRAALDPGLISDAVSMSALHICIQTYLFLNHICASTFTRYTNIAAHLA 219
           ATPFD  +G VN  AALDPGLI D      +            +   T T      A ++
Sbjct: 669 ATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAPVVFNYTGTNCLRNNATIS 728

Query: 220 TSAWAIPGISTTHQAPSSILWELKTVTSTVTNVAEADTSTITARMDPAVAIETNPPAMAL 245
            S   +P I+       S L   +TV   +TN+A  +T T++      V I  +P   ++
Sbjct: 729 GSDLNLPSITV------SKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSI 788

The following BLAST results are available for this feature:
BLAST of Spg017554 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022996483.12.5e-4551.91subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima] >XP_022996484.1 su... [more]
XP_022996485.12.5e-4551.91subtilisin-like protease SBT2.5 isoform X2 [Cucurbita maxima][more]
XP_022966056.15.5e-4551.34subtilisin-like protease SBT2.5 [Cucurbita maxima] >XP_022966057.1 subtilisin-li... [more]
KAG6598892.15.5e-4550.57Subtilisin-like protease 2.5, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022937793.15.5e-4551.34subtilisin-like protease SBT2.5 [Cucurbita moschata] >XP_022937794.1 subtilisin-... [more]
BLAST of Spg017554 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O644818.0e-3945.42Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S... [more]
Q9SZV51.2e-3744.27Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S... [more]
Q9FI125.6e-1638.89Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 S... [more]
Q9SUN66.8e-1429.60Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 S... [more]
Q9SA755.8e-1332.06Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 S... [more]
BLAST of Spg017554 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KAV81.2e-4551.91subtilisin-like protease SBT2.5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1K6W71.2e-4551.91subtilisin-like protease SBT2.5 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1HNB42.7e-4551.34subtilisin-like protease SBT2.5 OS=Cucurbita maxima OX=3661 GN=LOC111465779 PE=3... [more]
A0A1S3BRZ62.7e-4551.91subtilisin-like protease SBT2.5 OS=Cucumis melo OX=3656 GN=LOC103492606 PE=3 SV=... [more]
A0A6J1FC862.7e-4551.34subtilisin-like protease SBT2.5 OS=Cucurbita moschata OX=3662 GN=LOC111444081 PE... [more]
BLAST of Spg017554 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G19170.15.7e-4045.42subtilisin-like serine protease 3 [more]
AT4G30020.18.2e-3944.27PA-domain containing subtilase family protein [more]
AT5G44530.14.0e-1738.89Subtilase family protein [more]
AT4G20430.14.9e-1529.60Subtilase family protein [more]
AT4G20430.24.9e-1529.60Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 29..110
e-value: 7.0E-10
score: 38.7
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 15..145
e-value: 3.0E-15
score: 58.1
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 41..135
NoneNo IPR availablePANTHERPTHR10795:SF601CUCUMISIN-RELATEDcoord: 28..96
NoneNo IPR availablePANTHERPTHR10795:SF601CUCUMISIN-RELATEDcoord: 100..238
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 1..131
score: 10.049248
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 28..96
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 100..238
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 80..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017554.1Spg017554.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity