Spg017170 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg017170
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold4: 34663840 .. 34666227 (-)
RNA-Seq ExpressionSpg017170
SyntenySpg017170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATACATGTGAAGAATCTTCCCATTCCAAACTGGGTTCAATCCAACAAAAAAAACGAACCCACCCACGATTCTCAAATTTCTATCCAATTCCCCCATTTCAATTTCTTTTCATCTCCTTCCTCTTGCAATTGCACTTTTCATCGGCGTCCATGGCTGACCACAAGCCCATGGACCTGCTCAAGAAAGACCTCAAGAACTTGCTCGATAGAATCGTTGTTGAAGAAGATTTCGATTTCTCAACCGCCGACGAGGCTATCGAGAAATTGACTGCTTTGAAAATCTCCAAAATTGCCGCCGCCTCGCCGGAATCCGGTGAGAGTAGTGGCAGTACGACTCTGGTTTTGCCGGAGATGTTCCTGTGCCCGATTTCCGGCGAGTTCATGAAAGACCCTGTTCTCTTACAAACTGGTCAGGTTAGAATATTTTTTTGAAATCGTTTGATTTCTCTTCTGTTTTCTTGCGATTGGGTCTTGGAATTTTGTTGGGTCTTGATCGGTTTTGAGGAATTTTCATCGGTTGGAGGAGTCTTTGTGAATTAAGATTGTGTTGCTATCGCGAATTTGTGTATCTGTGGGCTCTGTTTCGTTTTGCTGCTTCTTTTTCTCTTTTTAACCGTGGGCTCTTATCTTTTGTGTTAATCATTACAATTCTTTAGTCTTTCAAAAAGAAGATGATGATTCAATGCTCCAATGCCTCCCATTGTCTAATATTAATGATTACAATTCTTTGTTTCAGAAAGTTAACTTTGCCTCTCGCATTGCCCTTTTGCCTCATATATTACTTTCTTTATGTGTTAAAAGATATCAAATATAAAGAACCTGTGTTCAAAGCCAATGTCCAATTCTAAAGTGATTGGGTCTTGTGTGTTAATTTTCTGAGGGGAAGTGTTATGAATATGTATAAAAGATTGAATATGCCATAACCTGGTGTTTTTGATAATTTTTGTGAGGGAAGTGTTATGAATATTATTTATGTGAGTTGAAGATTGAGTTTGTGCATAATGTTCTTGAAACATGGTTTTGAATTGCAGACATATGATCGGTCCTCCATCGAAAAGTGGTTCCATAAAGGGCACAATACATGTCCTAAGACCAAGGAAGTCCTGTCCGACATGACTCTTACTCCGAATCTTCTTCTTCGGTCGATGATGATGCAATGGTACGCGGAGAACGGATTGCCTATTCCGGATGTTATTTTCGAGGAAGCGATCGCCTATGCGGATGAATCCCGTTTGGATGAATTGCTTAACAAGTTGTTGTCTCAGTCTCTTTCGGATCAAAAAGAAGCTGCAAAGGAGTTGAGACGGATAGCGAGATTGAACCACGAGTTTCGAGCCCTTTTCGCAAATCTGCCCGGGTCCATTGAAAGATTATTGCATCCTCTCTCGGTAGAGAACGTTAATCTCCATCCTGATCTTCAGGAGGATTTGATTACCACTGTTCTGAATATCTCAATTCATGATGCTAACAAGAAATATTTAGCGGAGGAACCGTTGGTCCTCCCTTTGCTGATCCAATCTTTACAATACGGAAGTATCGAGGTGAAAGCGAATGCAGCTGCAGCTATATTCTCATTAGCTTTTCACGAGGCGAATAAGAACATGATTGGAAACGCTGGCGCTCTCAAACATCTGATTCTACTTTTAGACGAAGGCCATCCTGTAGTGATAAGAGATGCAGCTTCAGCAATTTTCCAACTCTGTACCACCCTCGAGAACAAAGAGAAGGCGGTTTGCAGCGGAGCAGTTGGTTCCATTGTTCGAAACATCAGTCAAGGCATAATGGTCGATGAGTTGGTTTCGGTACTTGCACTGCTCTCCACCAATCTCAAGGCTATCGACGAAATGTGCAAGCTCAAAACCGTGCCGTGTATGCTGAGAATCATGAGGAAAACTACCTCCCAAGGCGTCAAAGAAAACTGCGCTTCGATACTGTTTATAATCTGCACAGCCGACCGAAGCAAATGGACGGAGATTCAGGACGACGAAAATGCAAACGGTACCATCTTTGATCTTTCTCAGACCGGGACATCGAGGGCAAGGAGAAAGGCTTTGGGCATACTCGAAAAGCTGCGTAGAGCTGCTGCAATTACTCACACTGCTTAATGAAAATAGGTCAGATTCTTTTACACCAATTAACCTGTGTATAGATAGATTTAGGTATATACAGTGTCTTGCATTGCAATACAAAAAGTTGTGCATAATACAAAATATTAGTTGATATAGGAAATTTTGGTCCAAACCAAAGTGTGATGAATGAGAAAAACAGATTCAACATTTTTTTACTGTACATGCTAATAGTGAAGCTTTGGTCCAACCCAAGTGATGAATGAGAATTGCAATATCACCTTTTTGGTTTAAATTCTATTAATTTTCATCCTTATTTTT

mRNA sequence

ATGGCTGACCACAAGCCCATGGACCTGCTCAAGAAAGACCTCAAGAACTTGCTCGATAGAATCGTTGTTGAAGAAGATTTCGATTTCTCAACCGCCGACGAGGCTATCGAGAAATTGACTGCTTTGAAAATCTCCAAAATTGCCGCCGCCTCGCCGGAATCCGGTGAGAGTAGTGGCAGTACGACTCTGGTTTTGCCGGAGATGTTCCTGTGCCCGATTTCCGGCGAGTTCATGAAAGACCCTGTTCTCTTACAAACTGGTCAGACATATGATCGGTCCTCCATCGAAAAGTGGTTCCATAAAGGGCACAATACATGTCCTAAGACCAAGGAAGTCCTGTCCGACATGACTCTTACTCCGAATCTTCTTCTTCGGTCGATGATGATGCAATGGTACGCGGAGAACGGATTGCCTATTCCGGATGTTATTTTCGAGGAAGCGATCGCCTATGCGGATGAATCCCGTTTGGATGAATTGCTTAACAAGTTGTTGTCTCAGTCTCTTTCGGATCAAAAAGAAGCTGCAAAGGAGTTGAGACGGATAGCGAGATTGAACCACGAGTTTCGAGCCCTTTTCGCAAATCTGCCCGGGTCCATTGAAAGATTATTGCATCCTCTCTCGGTAGAGAACGTTAATCTCCATCCTGATCTTCAGGAGGATTTGATTACCACTGTTCTGAATATCTCAATTCATGATGCTAACAAGAAATATTTAGCGGAGGAACCGTTGGTCCTCCCTTTGCTGATCCAATCTTTACAATACGGAAGTATCGAGGTGAAAGCGAATGCAGCTGCAGCTATATTCTCATTAGCTTTTCACGAGGCGAATAAGAACATGATTGGAAACGCTGGCGCTCTCAAACATCTGATTCTACTTTTAGACGAAGGCCATCCTGTAGTGATAAGAGATGCAGCTTCAGCAATTTTCCAACTCTGTACCACCCTCGAGAACAAAGAGAAGGCGGTTTGCAGCGGAGCAGTTGGTTCCATTGTTCGAAACATCAGTCAAGGCATAATGGTCGATGAGTTGGTTTCGGTACTTGCACTGCTCTCCACCAATCTCAAGGCTATCGACGAAATGTGCAAGCTCAAAACCGTGCCGTGTATGCTGAGAATCATGAGGAAAACTACCTCCCAAGGCGTCAAAGAAAACTGCGCTTCGATACTGTTTATAATCTGCACAGCCGACCGAAGCAAATGGACGGAGATTCAGGACGACGAAAATGCAAACGGTACCATCTTTGATCTTTCTCAGACCGGGACATCGAGGGCAAGGAGAAAGGCTTTGGGCATACTCGAAAAGCTGCGTAGAGCTGCTGCAATTACTCACACTGCTTAA

Coding sequence (CDS)

ATGGCTGACCACAAGCCCATGGACCTGCTCAAGAAAGACCTCAAGAACTTGCTCGATAGAATCGTTGTTGAAGAAGATTTCGATTTCTCAACCGCCGACGAGGCTATCGAGAAATTGACTGCTTTGAAAATCTCCAAAATTGCCGCCGCCTCGCCGGAATCCGGTGAGAGTAGTGGCAGTACGACTCTGGTTTTGCCGGAGATGTTCCTGTGCCCGATTTCCGGCGAGTTCATGAAAGACCCTGTTCTCTTACAAACTGGTCAGACATATGATCGGTCCTCCATCGAAAAGTGGTTCCATAAAGGGCACAATACATGTCCTAAGACCAAGGAAGTCCTGTCCGACATGACTCTTACTCCGAATCTTCTTCTTCGGTCGATGATGATGCAATGGTACGCGGAGAACGGATTGCCTATTCCGGATGTTATTTTCGAGGAAGCGATCGCCTATGCGGATGAATCCCGTTTGGATGAATTGCTTAACAAGTTGTTGTCTCAGTCTCTTTCGGATCAAAAAGAAGCTGCAAAGGAGTTGAGACGGATAGCGAGATTGAACCACGAGTTTCGAGCCCTTTTCGCAAATCTGCCCGGGTCCATTGAAAGATTATTGCATCCTCTCTCGGTAGAGAACGTTAATCTCCATCCTGATCTTCAGGAGGATTTGATTACCACTGTTCTGAATATCTCAATTCATGATGCTAACAAGAAATATTTAGCGGAGGAACCGTTGGTCCTCCCTTTGCTGATCCAATCTTTACAATACGGAAGTATCGAGGTGAAAGCGAATGCAGCTGCAGCTATATTCTCATTAGCTTTTCACGAGGCGAATAAGAACATGATTGGAAACGCTGGCGCTCTCAAACATCTGATTCTACTTTTAGACGAAGGCCATCCTGTAGTGATAAGAGATGCAGCTTCAGCAATTTTCCAACTCTGTACCACCCTCGAGAACAAAGAGAAGGCGGTTTGCAGCGGAGCAGTTGGTTCCATTGTTCGAAACATCAGTCAAGGCATAATGGTCGATGAGTTGGTTTCGGTACTTGCACTGCTCTCCACCAATCTCAAGGCTATCGACGAAATGTGCAAGCTCAAAACCGTGCCGTGTATGCTGAGAATCATGAGGAAAACTACCTCCCAAGGCGTCAAAGAAAACTGCGCTTCGATACTGTTTATAATCTGCACAGCCGACCGAAGCAAATGGACGGAGATTCAGGACGACGAAAATGCAAACGGTACCATCTTTGATCTTTCTCAGACCGGGACATCGAGGGCAAGGAGAAAGGCTTTGGGCATACTCGAAAAGCTGCGTAGAGCTGCTGCAATTACTCACACTGCTTAA

Protein sequence

MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSLSDQKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA
Homology
BLAST of Spg017170 vs. NCBI nr
Match: XP_022965721.1 (U-box domain-containing protein 9-like [Cucurbita maxima])

HSP 1 Score: 577.4 bits (1487), Expect = 1.1e-160
Identity = 305/456 (66.89%), Postives = 366/456 (80.26%), Query Frame = 0

Query: 1   MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K+LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAA 60

Query: 61  ---ESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKT 120
              ESG  SG T LV+P+ F CPISGE M+DPVLL+TGQTYDR  I KWF+KGHNTCP+T
Sbjct: 61  PPLESGGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRT 120

Query: 121 KEVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSLS 180
            EVL DMTLTPN  LRSM+++W  +N L +P + +EE +A+A ES LDELLN+LLS SLS
Sbjct: 121 NEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLS 180

Query: 181 DQKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNIS 240
           DQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS
Sbjct: 181 DQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNIS 240

Query: 241 IHDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHL 300
           +   NKK L E  LVL LLIQSLQYGS E KANAAAAIFSL+ ++ NK  IGNAGA+KHL
Sbjct: 241 LSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHL 300

Query: 301 ILLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLAL 360
           + LLDEGHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLAL
Sbjct: 301 VGLLDEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLAL 360

Query: 361 LSTNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENA 420
           LS N+KAI  MCKL  V CMLR++R++TSQGVKENC SIL  I +  RSK  EIQ+DENA
Sbjct: 361 LSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENA 420

Query: 421 NGTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
           NG I  LS+ G SRA+RKA+ +LEKL+RA +ITHTA
Sbjct: 421 NGMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of Spg017170 vs. NCBI nr
Match: KAG7021008.1 (U-box domain-containing protein 9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 575.1 bits (1481), Expect = 5.3e-160
Identity = 305/458 (66.59%), Postives = 364/458 (79.48%), Query Frame = 0

Query: 1   MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAASAAAAAA 60

Query: 61  -----ESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCP 120
                ESG  S  T LV+P+ F CPISGE M+DPVLL+TGQTYDR  I KWF+KGHNTCP
Sbjct: 61  AAAPVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCP 120

Query: 121 KTKEVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQS 180
           +T EVL DMTLTPN  LRSM+++W  +N L +P + +EE +A+A ES LDELLN+LLS S
Sbjct: 121 RTNEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSS 180

Query: 181 LSDQKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLN 240
           LSDQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLN
Sbjct: 181 LSDQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLN 240

Query: 241 ISIHDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALK 300
           IS+   NKK L E  LVL LLIQSLQYGS E KANAAAAIFSL+ ++ NK  IGNAGA+K
Sbjct: 241 ISLSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIK 300

Query: 301 HLILLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVL 360
           HL+ LLDEGHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVL
Sbjct: 301 HLVGLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVL 360

Query: 361 ALLSTNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDE 420
           ALLS N+KAI  MCKL  V CMLR++R++TSQGVKENC SIL  I +  RSK  EIQ+DE
Sbjct: 361 ALLSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDE 420

Query: 421 NANGTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
           NANG I  LSQ G SRA+RKA+ +LEKL+RA +ITHTA
Sbjct: 421 NANGMISKLSQNGNSRAKRKAMSVLEKLQRAGSITHTA 458

BLAST of Spg017170 vs. NCBI nr
Match: XP_022937712.1 (U-box domain-containing protein 9-like [Cucurbita moschata])

HSP 1 Score: 574.7 bits (1480), Expect = 6.9e-160
Identity = 304/455 (66.81%), Postives = 364/455 (80.00%), Query Frame = 0

Query: 1   MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAAS         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAASAAAAAAAAA 60

Query: 61  --ESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTK 120
             ESG  S  T LV+P+ F CPISGE M+DPVLL+TGQTYDR  I KWF+KGHNTCP+T 
Sbjct: 61  PVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTN 120

Query: 121 EVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSLSD 180
           EVL DMTLTPN  LRSM+++W  +N L +P + +EE +A+A ES LDELLN+LLS SLSD
Sbjct: 121 EVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSD 180

Query: 181 QKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISI 240
           QK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS+
Sbjct: 181 QKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNISL 240

Query: 241 HDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLI 300
              NKK L E  LVL LLIQSLQYGS E KANAAAAIFSL+ ++ NK  IGNAGA+KHL+
Sbjct: 241 SGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLV 300

Query: 301 LLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALL 360
            LLDEGHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLALL
Sbjct: 301 GLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLALL 360

Query: 361 STNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENAN 420
           S N+KAI  MCKL  V CML+++R++TSQGVKENC SIL  I +  RSK  EIQ+DENAN
Sbjct: 361 SNNMKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENAN 420

Query: 421 GTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
           G I  LS+ G SRA+RKA+ +LEKL+RA +ITHTA
Sbjct: 421 GMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 455

BLAST of Spg017170 vs. NCBI nr
Match: XP_023538150.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.8 bits (1475), Expect = 2.6e-159
Identity = 304/457 (66.52%), Postives = 363/457 (79.43%), Query Frame = 0

Query: 1   MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAA 60

Query: 61  ----ESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPK 120
               ESG  SG   LV+P+ F CPISGE M+DPVLL+TGQTYDR  I KWF+KGHNTCP+
Sbjct: 61  AAPVESGGDSGRKALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPR 120

Query: 121 TKEVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSL 180
           T EVL DMTLTPN  LRSM+++W  +N L +P + +EE +A+A ES LDELLN+LLS SL
Sbjct: 121 TNEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSL 180

Query: 181 SDQKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNI 240
           SDQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNI
Sbjct: 181 SDQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNI 240

Query: 241 SIHDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKH 300
           S+   NKK L E  LVL LLIQSLQYGS E KANAAAAIFSL+ ++ NK  IGNAGA+KH
Sbjct: 241 SLSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKH 300

Query: 301 LILLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLA 360
           L+ LLDEGHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLA
Sbjct: 301 LVGLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLA 360

Query: 361 LLSTNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDEN 420
           LLS N+KAI  MCKL  V CMLR++R++TSQGVKENC SIL  I +  RSK  EIQ+DEN
Sbjct: 361 LLSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDEN 420

Query: 421 ANGTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
           ANG I  LS+ G SRA+RKA  +LEKL+RA +ITHTA
Sbjct: 421 ANGMISKLSRNGNSRAKRKATSVLEKLQRAGSITHTA 457

BLAST of Spg017170 vs. NCBI nr
Match: KAG6586176.1 (U-box domain-containing protein 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 572.0 bits (1473), Expect = 4.5e-159
Identity = 303/459 (66.01%), Postives = 364/459 (79.30%), Query Frame = 0

Query: 1   MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAASAAAAAA 60

Query: 61  ------ESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTC 120
                 ESG  S  T LV+P+ F CPISGE M+DPVLL+TGQTYDR  I KWF+KGHNTC
Sbjct: 61  AAAAPVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTC 120

Query: 121 PKTKEVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQ 180
           P+T EVL DMTLTPN  LRSM+++W  +N L +P + +EE +A+A ES LDELLN+LLS 
Sbjct: 121 PRTNEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSS 180

Query: 181 SLSDQKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVL 240
           SLSDQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVL
Sbjct: 181 SLSDQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVL 240

Query: 241 NISIHDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGAL 300
           NIS+   NKK L E  LVL LLIQSLQYGS E KANAAAAIFSL+ ++ NK  IGNAGA+
Sbjct: 241 NISLSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAI 300

Query: 301 KHLILLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSV 360
           KHL+ LLDEGHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSV
Sbjct: 301 KHLVGLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSV 360

Query: 361 LALLSTNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDD 420
           LALLS N+KAI  MCKL  V CML+++R++TSQGVKENC SIL  I +  RSK  EIQ+D
Sbjct: 361 LALLSNNMKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQED 420

Query: 421 ENANGTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
           ENANG I  LS+ G SRA+RKA+ +LEKL+RA +ITHTA
Sbjct: 421 ENANGMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 459

BLAST of Spg017170 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.1e-99
Identity = 207/443 (46.73%), Postives = 300/443 (67.72%), Query Frame = 0

Query: 10  LKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSTTLVLPEMF 69
           LK+++K LL +I  E+D    T D+  + L+AL+ + +   + +S       T+  PE F
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKMA-KSSSLEMLETVSCPEEF 79

Query: 70  LCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRSMMM 129
            CP+S E M+DPV+L +GQTYD+  I+KW   G+ TCPKT++VL    LTPNLL+R M+ 
Sbjct: 80  RCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIREMIS 139

Query: 130 QWYAENGLPI-----PDVIFE-EAIAYADESRLDELLNKLLSQSLSDQKEAAKELRRIAR 189
           +W  +NGL       P+++ E E +  +D    + LL K+ S +L DQK AAKELR + R
Sbjct: 140 KWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRLLTR 199

Query: 190 LNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLAEEP 249
              EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + E P
Sbjct: 200 KGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVCENP 259

Query: 250 LVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPVVIR 309
            V+PLLI +L+ G++  ++NAAAAIF+L+  ++NK +IG +G LK LI LL+EG+P+ I+
Sbjct: 260 NVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPLAIK 319

Query: 310 DAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDEMCK 369
           D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E+ +
Sbjct: 320 DVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEELGE 379

Query: 370 LKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQTGTS 429
           L  V  +L+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ GTS
Sbjct: 380 LGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSREGTS 439

Query: 430 RARRKALGILEKLRRAAAITHTA 446
           RA+RKA GIL++LR+A  +THTA
Sbjct: 440 RAQRKANGILDRLRKAMNLTHTA 460

BLAST of Spg017170 vs. ExPASy Swiss-Prot
Match: Q3E9F7 (Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=PUB46 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 5.6e-64
Identity = 170/447 (38.03%), Postives = 264/447 (59.06%), Query Frame = 0

Query: 8   DLLKKDLKNLLDRIVVE---EDFDFSTA--------DEAIEKLTALKISKIAAASPESGE 67
           D L+++L+ +L  I+ +    D D + A        DEA+  LT L+  K+ +  PES  
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLR--KVESKIPESDI 69

Query: 68  SSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDM 127
           S     + +P+ F+C +S   M +PV++ +GQTY++  I +W  K   TCPKTK+VLS  
Sbjct: 70  S----PVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWL-KHERTCPKTKQVLSHR 129

Query: 128 TLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKL-LSQSLSDQKEAA 187
              PN L+  ++ QW   N         +E +A    S ++ LL ++  S S++DQ EAA
Sbjct: 130 LWIPNHLISDLITQWCLVNKYD-HQKPSDELVAELFTSDIEALLQRVSSSSSVADQIEAA 189

Query: 188 KELRRIARLNHEFRALF-ANLPGSIERLLHPLSV--ENVNLHPDLQEDLITTVLNISIHD 247
           KELR   +     R  F A +  SI RLL PLS   E V+   +LQE+++T + N+SI +
Sbjct: 190 KELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSILE 249

Query: 248 ANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILL 307
           +NK  +AE  LV+PLL +SL+ G+ E + NAAA + SL+  ++NK +IGN+ A+K LI L
Sbjct: 250 SNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALIDL 309

Query: 308 LDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLST 367
           ++EG  +  ++A S +F LC  LENK K V +G + +  + I  G  VDEL+S+LAL+ST
Sbjct: 310 IEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIST 369

Query: 368 NLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIIC--TADRSKWTEIQDDENAN 427
           + +A++EM KL  +  +  I+RK +S    EN   I+F +     DRS+   + ++EN +
Sbjct: 370 HNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQH 429

Query: 428 GTIFDLSQTGTSRARRKALGILEKLRR 438
           GT   L++ G+ RA RKA GIL+ ++R
Sbjct: 430 GTFTKLAKQGSVRAARKAQGILQWIKR 448

BLAST of Spg017170 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.9e-60
Identity = 154/431 (35.73%), Postives = 247/431 (57.31%), Query Frame = 0

Query: 33  DEAIEKLTALKISKIAAASPESGESSGST---------TLVLPEMFLCPISGEFMKDPVL 92
           D+  E++++L  + +   + ES +   ST         + V+PE F CPIS E MKDPV+
Sbjct: 207 DDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVI 266

Query: 93  LQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRSMMMQWYAENGLPIPD-- 152
           + TGQTY+RSSI+KW   GH TCPK++E L    LTPN +L+S++  W   NG+ +P   
Sbjct: 267 VSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQ 326

Query: 153 ------VIFEEAIAYADESRLDELLNKLLSQSLSDQKEAAKELRRIARLNHEFRALFANL 212
                  I   + +  D + +  LL KL + +   Q+ AA ELR +A+ N + R   A  
Sbjct: 327 GSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAE- 386

Query: 213 PGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDANKKYLAEEPLVLPLLIQSLQYG 272
            G+I  L+  LS  +    P  QE  +T +LN+SI++ NK  + +   +   +++ L+ G
Sbjct: 387 AGAIPLLVELLSSPD----PRTQEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNG 446

Query: 273 SIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPVVIRDAASAIFQLCTTL 332
           S+E + NAAA +FSL+  + NK  IG AGA++ LI LL+EG     +DAA+AIF LC   
Sbjct: 447 SMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQ 506

Query: 333 ENKEKAVCSGAVGSIVRNISQ--GIMVDELVSVLALLSTNLKAIDEMCKLKTVPCMLRIM 392
            NK +AV  G V  + R +    G MVDE +++LA+LSTN +    + + +++P ++ I+
Sbjct: 507 GNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEII 566

Query: 393 RKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQTGTSRARRKALGILE 445
           R T S   +EN A+IL+ +C  +  +   +  +  A+  + +L++ GT RA+RKA  +LE
Sbjct: 567 R-TGSPRNRENAAAILWYLCIGNIER-LNVAREVGADVALKELTENGTDRAKRKAASLLE 626

BLAST of Spg017170 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 4.9e-60
Identity = 148/387 (38.24%), Postives = 235/387 (60.72%), Query Frame = 0

Query: 56  ESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSD 115
           ES  S  L +P  FLCP+S E MKDPV++ TGQTY+R+ I++W   G+ TCPKT++ L +
Sbjct: 232 ESKKSDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKTQQKLEN 291

Query: 116 MTLTPNLLLRSMMMQWYAENGLPIP-DVIFEEAIAYADESRLDELLNKLLSQSLSDQKEA 175
            TLTPN +LRS++ +W AE+ +  P   I        D S +  L+ +L S+S  D++ A
Sbjct: 292 FTLTPNYVLRSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLSSRSTEDRRNA 351

Query: 176 AKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDAN 235
             E+R +++ + + R L A   G+I  L++ L+ E+V      QE+ IT VLN+SI++ N
Sbjct: 352 VSEIRSLSKRSTDNRILIAE-AGAIPVLVNLLTSEDV----ATQENAITCVLNLSIYENN 411

Query: 236 KKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLD 295
           K+ +     V   ++Q L+ G++E + NAAA +FSL+  + NK +IG +GA+  L+ LL+
Sbjct: 412 KELIMFAGAVTS-IVQVLRAGTMEARENAAATLFSLSLADENKIIIGGSGAIPALVDLLE 471

Query: 296 EGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGI---MVDELVSVLALLS 355
            G P   +DAA+A+F LC    NK +AV +G V ++V+ +S      MVDE +++L++L+
Sbjct: 472 NGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVDEALTILSVLA 531

Query: 356 TNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANG 415
            N  A   + K  T+P ++ I++   ++  +EN A+IL  +C  D  K   I     A  
Sbjct: 532 NNQDAKSAIVKANTLPALIGILQTDQTRN-RENAAAILLSLCKRDTEKLITI-GRLGAVV 591

Query: 416 TIFDLSQTGTSRARRKALGILEKLRRA 439
            + DLS+ GT R +RKA+ +LE LR+A
Sbjct: 592 PLMDLSKNGTERGKRKAISLLELLRKA 610

BLAST of Spg017170 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.4e-59
Identity = 161/441 (36.51%), Postives = 254/441 (57.60%), Query Frame = 0

Query: 7   MDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSTTLVLP 66
           +D +   LK L D +V E        D A + LT  + + I   SP           ++P
Sbjct: 190 VDQMSSLLKKLKDCVVTE--------DHANDALTT-RSASIKHRSP-----------IIP 249

Query: 67  EMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRS 126
           + F CPIS E M+DPV++ +GQTY+RS I+KW   GH TCPKT++ LS  +LTPN +L+S
Sbjct: 250 DEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFVLKS 309

Query: 127 MMMQWYAENGLPIP-------DVIFEEAIAYADESRLDELLNKLLSQSLSDQKEAAKELR 186
           ++ QW   NG+ +P       D    ++  Y D + L  L+N+L S +  +Q+ AA E+R
Sbjct: 310 LISQWCEANGIELPKNKQNSRDKKAAKSSDY-DHAGLVSLMNRLRSGNQDEQRAAAGEIR 369

Query: 187 RIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDANKKYLA 246
            +A+ N   R   A   G+I  L++ LS  +    P  QE  +T +LN+SIH+ NK  + 
Sbjct: 370 LLAKRNVNNRICIAE-AGAIPLLVNLLSSSD----PRTQEHAVTALLNLSIHENNKASIV 429

Query: 247 EEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPV 306
           +    +P +++ L+ GS+E + NAAA +FSL+  + NK  IG AGA+  LI LL +G P 
Sbjct: 430 DSH-AIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGSPR 489

Query: 307 VIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNI--SQGIMVDELVSVLALLSTNLKAI 366
             +DAA+AIF LC    NK +AV +G V  ++  +    G M+DE +S+L++L+ N +  
Sbjct: 490 GKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNPEGK 549

Query: 367 DEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLS 426
             + + + +P ++ ++ KT S   +EN A+IL+++C+AD ++ T           + +LS
Sbjct: 550 IVIARSEPIPPLVEVI-KTGSPRNRENAAAILWLLCSAD-TEQTLAAKAAGVEDALKELS 601

Query: 427 QTGTSRARRKALGILEKLRRA 439
           +TGT RA+RKA  ILE + +A
Sbjct: 610 ETGTDRAKRKASSILELMHQA 601

BLAST of Spg017170 vs. ExPASy TrEMBL
Match: A0A6J1HMF9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465532 PE=4 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 5.1e-161
Identity = 305/456 (66.89%), Postives = 366/456 (80.26%), Query Frame = 0

Query: 1   MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K+LL RIV ++DF+ S ADEAIEKL +LK++K+AAA+         
Sbjct: 1   MADKESLELLKKDIKDLLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAAAAAAAAAAAA 60

Query: 61  ---ESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKT 120
              ESG  SG T LV+P+ F CPISGE M+DPVLL+TGQTYDR  I KWF+KGHNTCP+T
Sbjct: 61  PPLESGGDSGRTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRT 120

Query: 121 KEVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSLS 180
            EVL DMTLTPN  LRSM+++W  +N L +P + +EE +A+A ES LDELLN+LLS SLS
Sbjct: 121 NEVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLS 180

Query: 181 DQKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNIS 240
           DQK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS
Sbjct: 181 DQKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNIS 240

Query: 241 IHDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHL 300
           +   NKK L E  LVL LLIQSLQYGS E KANAAAAIFSL+ ++ NK  IGNAGA+KHL
Sbjct: 241 LSGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHL 300

Query: 301 ILLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLAL 360
           + LLDEGHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLAL
Sbjct: 301 VGLLDEGHPVVMRDAASAIFSLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLAL 360

Query: 361 LSTNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENA 420
           LS N+KAI  MCKL  V CMLR++R++TSQGVKENC SIL  I +  RSK  EIQ+DENA
Sbjct: 361 LSNNMKAISIMCKLNAVSCMLRVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENA 420

Query: 421 NGTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
           NG I  LS+ G SRA+RKA+ +LEKL+RA +ITHTA
Sbjct: 421 NGMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 456

BLAST of Spg017170 vs. ExPASy TrEMBL
Match: A0A6J1FC00 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444033 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 3.3e-160
Identity = 304/455 (66.81%), Postives = 364/455 (80.00%), Query Frame = 0

Query: 1   MADHKPMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASP-------- 60
           MAD + ++LLKKD+K LL RIV ++DF+ S ADEAIEKL +LK++K+AAAS         
Sbjct: 1   MADKESLELLKKDIKELLQRIVDQDDFECSIADEAIEKLNSLKLTKMAAASAAAAAAAAA 60

Query: 61  --ESGESSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTK 120
             ESG  S  T LV+P+ F CPISGE M+DPVLL+TGQTYDR  I KWF+KGHNTCP+T 
Sbjct: 61  PVESGGDSARTALVMPDEFRCPISGELMEDPVLLKTGQTYDRPFINKWFNKGHNTCPRTN 120

Query: 121 EVLSDMTLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSLSD 180
           EVL DMTLTPN  LRSM+++W  +N L +P + +EE +A+A ES LDELLN+LLS SLSD
Sbjct: 121 EVLHDMTLTPNRSLRSMILKWCIDNKLELPKLTYEETVAHATESHLDELLNQLLSSSLSD 180

Query: 181 QKEAAKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISI 240
           QK AAKELR+IA+ NHEFR+LFA LP SIERLLHP+SV++VNLHPDLQEDLITTVLNIS+
Sbjct: 181 QKLAAKELRQIAKWNHEFRSLFAKLPDSIERLLHPISVDHVNLHPDLQEDLITTVLNISL 240

Query: 241 HDANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLI 300
              NKK L E  LVL LLIQSLQYGS E KANAAAAIFSL+ ++ NK  IGNAGA+KHL+
Sbjct: 241 SGDNKKRLVENQLVLSLLIQSLQYGSSEAKANAAAAIFSLSLYQENKIAIGNAGAIKHLV 300

Query: 301 LLLDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALL 360
            LLDEGHPVV+RDAASAIF LCT   NKEKAV SG V S++RNI++G++VDELVSVLALL
Sbjct: 301 GLLDEGHPVVMRDAASAIFTLCTASANKEKAVSSGVVVSVLRNITEGVLVDELVSVLALL 360

Query: 361 STNLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENAN 420
           S N+KAI  MCKL  V CML+++R++TSQGVKENC SIL  I +  RSK  EIQ+DENAN
Sbjct: 361 SNNMKAISIMCKLNAVSCMLKVIRESTSQGVKENCISILDTISSTYRSKLKEIQEDENAN 420

Query: 421 GTIFDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
           G I  LS+ G SRA+RKA+ +LEKL+RA +ITHTA
Sbjct: 421 GMISKLSRNGNSRAKRKAMSVLEKLQRAGSITHTA 455

BLAST of Spg017170 vs. ExPASy TrEMBL
Match: A0A6J1CT87 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111014117 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 4.4e-152
Identity = 296/452 (65.49%), Postives = 363/452 (80.31%), Query Frame = 0

Query: 1   MADHK-PMDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSG 60
           MA+ K P + +KKDL+ LL++I  +ED++  +ADEAI++L ALK+S+I  AS  +  SS 
Sbjct: 1   MAEEKPPSEPIKKDLEKLLNKITHQEDWEIVSADEAIDRLDALKLSRI--ASTAACGSSR 60

Query: 61  STTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLT 120
           S +  +PE F CPISGE MKDPV+L+TGQTYDR+ IEKWFHKGHNTCP+TKEVLSDMTLT
Sbjct: 61  SRSPFVPEFFRCPISGELMKDPVILKTGQTYDRAFIEKWFHKGHNTCPQTKEVLSDMTLT 120

Query: 121 PNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLL-----SQSLSDQKEA 180
           PN LLR ++ QW AENGL +P ++FEE +    E   DELLNKL      S SLSDQK A
Sbjct: 121 PNRLLREIISQWCAENGLELPKLVFEEVVTSESECDFDELLNKLSLSLSPSSSLSDQKAA 180

Query: 181 AKELRRIARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDAN 240
           AKELRR+A+ NHEFRALFA  P SI+RLL  L ++N++LHPDLQEDL+T+VLNISIH+ N
Sbjct: 181 AKELRRLAKGNHEFRALFAKRPESIDRLLSCLLLDNISLHPDLQEDLVTSVLNISIHEDN 240

Query: 241 KKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLL- 300
           KK++AE  L LP+LI+SLQYG+   KANAAAAI SLA  E+NK +IGNAGALK LI LL 
Sbjct: 241 KKHVAENWLALPVLIESLQYGNTNTKANAAAAISSLACLESNKIIIGNAGALKPLIALLD 300

Query: 301 DEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTN 360
           D+ HPVVI+DAASAIF+LCT  +NKE+AV +GAVGS++RNI  GIMVDELVSVLALLS+N
Sbjct: 301 DDAHPVVIKDAASAIFRLCTIHQNKEQAVYNGAVGSVLRNIIGGIMVDELVSVLALLSSN 360

Query: 361 LKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTI 420
            KA++EMCKL TVPCML IMR+ +++ VKENC SILF ICT++R K  EIQ+DEN NGTI
Sbjct: 361 RKAVEEMCKLDTVPCMLEIMREASAECVKENCVSILFAICTSNRRKMWEIQEDENWNGTI 420

Query: 421 FDLSQTGTSRARRKALGILEKLRRAAAITHTA 446
            +LS +G SRARRKA+GILE+L RAA+ THTA
Sbjct: 421 VELSTSGNSRARRKAIGILERLHRAASRTHTA 450

BLAST of Spg017170 vs. ExPASy TrEMBL
Match: A0A5A7UZN6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold165G00400 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.5e-149
Identity = 284/442 (64.25%), Postives = 349/442 (78.96%), Query Frame = 0

Query: 7   MDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKI-AAASPESGESSGSTTLVL 66
           MDLLKKDLK +L RI+ EEDFD S AD+AI+ LT+LK S + A +S ES   SGS TL++
Sbjct: 3   MDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTLII 62

Query: 67  PEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLR 126
           P+ F CPISG+ MKDPVLL TGQTYDR  IEKWFH+GHNTCP+TKEVL+DMTLTPN LLR
Sbjct: 63  PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRLLR 122

Query: 127 SMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSLSDQKEAAKELRRIARLN 186
           SM+ QW  +N   +P + +EE +    ES LD LLN+L+S SL  +KEAAKELR   R +
Sbjct: 123 SMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTRCS 182

Query: 187 HEFRALFANLPGSIERLLHPL-SVENVNLHPDLQEDLITTVLNISIHDANKKYLAEEPLV 246
           HEFRALFA LPGS+ERLLHPL S+  V+LHPDLQEDLITT+LNIS+ D NK+++ E PLV
Sbjct: 183 HEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENPLV 242

Query: 247 LPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPVVIRDA 306
           LPLLI+SLQ+GSIE+ ANA AAI+SL+F EANK  +G  G  KHLI LLD  HP V RDA
Sbjct: 243 LPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTRDA 302

Query: 307 ASAIFQLCTTLENKEKAVCSGAVGSIVRNISQG-IMVDELVSVLALLSTNLKAIDEMCKL 366
            SAI+ LCTT+EN+EKAV SG V +I RNI++  ++VD+L+S+LALL T++KAIDEMCK 
Sbjct: 303 GSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMCKF 362

Query: 367 KTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQTGTSR 426
             VPCMLR +R+T SQ +KENCASILF ICT D+S+  +IQ+DEN N TI +LS+ G SR
Sbjct: 363 DVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGNSR 422

Query: 427 ARRKALGILEKLRRAAAITHTA 446
           A RKA GILE+++RAA+ THT+
Sbjct: 423 ASRKATGILERMQRAASRTHTS 444

BLAST of Spg017170 vs. ExPASy TrEMBL
Match: A0A1S3B4X7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485858 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.5e-149
Identity = 284/442 (64.25%), Postives = 349/442 (78.96%), Query Frame = 0

Query: 7   MDLLKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKI-AAASPESGESSGSTTLVL 66
           MDLLKKDLK +L RI+ EEDFD S AD+AI+ LT+LK S + A +S ES   SGS TL++
Sbjct: 3   MDLLKKDLKQVLYRIIQEEDFDSSAADDAIQILTSLKFSLMPAPSSQESPGCSGSKTLII 62

Query: 67  PEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLR 126
           P+ F CPISG+ MKDPVLL TGQTYDR  IEKWFH+GHNTCP+TKEVL+DMTLTPN LLR
Sbjct: 63  PDKFRCPISGDLMKDPVLLITGQTYDRFFIEKWFHEGHNTCPQTKEVLTDMTLTPNRLLR 122

Query: 127 SMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKLLSQSLSDQKEAAKELRRIARLN 186
           SM+ QW  +N   +P + +EE +    ES LD LLN+L+S SL  +KEAAKELR   R +
Sbjct: 123 SMISQWCLDNRFELPRLSYEEEVDNVTESHLDALLNRLMSSSLIVKKEAAKELRETTRCS 182

Query: 187 HEFRALFANLPGSIERLLHPL-SVENVNLHPDLQEDLITTVLNISIHDANKKYLAEEPLV 246
           HEFRALFA LPGS+ERLLHPL S+  V+LHPDLQEDLITT+LNIS+ D NK+++ E PLV
Sbjct: 183 HEFRALFAKLPGSVERLLHPLISIGKVDLHPDLQEDLITTILNISVFDDNKEHVVENPLV 242

Query: 247 LPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPVVIRDA 306
           LPLLI+SLQ+GSIE+ ANA AAI+SL+F EANK  +G  G  KHLI LLD  HP V RDA
Sbjct: 243 LPLLIESLQHGSIELTANAVAAIYSLSFSEANKITMGKVGIFKHLISLLDYAHPGVTRDA 302

Query: 307 ASAIFQLCTTLENKEKAVCSGAVGSIVRNISQG-IMVDELVSVLALLSTNLKAIDEMCKL 366
            SAI+ LCTT+EN+EKAV SG V +I RNI++  ++VD+L+S+LALL T++KAIDEMCK 
Sbjct: 303 GSAIYNLCTTVENREKAVGSGVVAAIFRNIARSLLLVDKLISILALLCTDVKAIDEMCKF 362

Query: 367 KTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQTGTSR 426
             VPCMLR +R+T SQ +KENCASILF ICT D+S+  +IQ+DEN N TI +LS+ G SR
Sbjct: 363 DVVPCMLRFIRETESQRIKENCASILFAICTTDQSQLRKIQEDENKNETILELSKNGNSR 422

Query: 427 ARRKALGILEKLRRAAAITHTA 446
           A RKA GILE+++RAA+ THT+
Sbjct: 423 ASRKATGILERMQRAASRTHTS 444

BLAST of Spg017170 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 365.2 bits (936), Expect = 7.7e-101
Identity = 207/443 (46.73%), Postives = 300/443 (67.72%), Query Frame = 0

Query: 10  LKKDLKNLLDRIVVEEDFDFSTADEAIEKLTALKISKIAAASPESGESSGSTTLVLPEMF 69
           LK+++K LL +I  E+D    T D+  + L+AL+ + +   + +S       T+  PE F
Sbjct: 20  LKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKMA-KSSSLEMLETVSCPEEF 79

Query: 70  LCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRSMMM 129
            CP+S E M+DPV+L +GQTYD+  I+KW   G+ TCPKT++VL    LTPNLL+R M+ 
Sbjct: 80  RCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQVLPHTALTPNLLIREMIS 139

Query: 130 QWYAENGLPI-----PDVIFE-EAIAYADESRLDELLNKLLSQSLSDQKEAAKELRRIAR 189
           +W  +NGL       P+++ E E +  +D    + LL K+ S +L DQK AAKELR + R
Sbjct: 140 KWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRLLTR 199

Query: 190 LNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLAEEP 249
              EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + E P
Sbjct: 200 KGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVCENP 259

Query: 250 LVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPVVIR 309
            V+PLLI +L+ G++  ++NAAAAIF+L+  ++NK +IG +G LK LI LL+EG+P+ I+
Sbjct: 260 NVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPLAIK 319

Query: 310 DAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDEMCK 369
           D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E+ +
Sbjct: 320 DVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEELGE 379

Query: 370 LKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQTGTS 429
           L  V  +L+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ GTS
Sbjct: 380 LGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSREGTS 439

Query: 430 RARRKALGILEKLRRAAAITHTA 446
           RA+RKA GIL++LR+A  +THTA
Sbjct: 440 RAQRKANGILDRLRKAMNLTHTA 460

BLAST of Spg017170 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 272.3 bits (695), Expect = 6.8e-73
Identity = 157/326 (48.16%), Postives = 226/326 (69.33%), Query Frame = 0

Query: 127 MMMQWYAENGLPI-----PDVIFE-EAIAYADESRLDELLNKLLSQSLSDQKEAAKELRR 186
           M+ +W  +NGL       P+++ E E +  +D    + LL K+ S +L DQK AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 187 IARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLA 246
           + R   EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + 
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVC 120

Query: 247 EEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPV 306
           E P V+PLLI +L+ G++  ++NAAAAIF+L+  ++NK +IG +G LK LI LL+EG+P+
Sbjct: 121 ENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPL 180

Query: 307 VIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDE 366
            I+D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E
Sbjct: 181 AIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEE 240

Query: 367 MCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQT 426
           + +L  V  +L+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ 
Sbjct: 241 LGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSRE 300

Query: 427 GTSRARRKALGILEKLRRAAAITHTA 446
           GTSRA+RKA GIL++LR+A  +THTA
Sbjct: 301 GTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Spg017170 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 272.3 bits (695), Expect = 6.8e-73
Identity = 157/326 (48.16%), Postives = 226/326 (69.33%), Query Frame = 0

Query: 127 MMMQWYAENGLPI-----PDVIFE-EAIAYADESRLDELLNKLLSQSLSDQKEAAKELRR 186
           M+ +W  +NGL       P+++ E E +  +D    + LL K+ S +L DQK AAKELR 
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 187 IARLNHEFRALFANLPGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIH-DANKKYLA 246
           + R   EFRALF   P  I RL++PL +   N    LQED++TT+LNISIH D+NKK + 
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPL-LHGSNPDEKLQEDVVTTLLNISIHDDSNKKLVC 120

Query: 247 EEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPV 306
           E P V+PLLI +L+ G++  ++NAAAAIF+L+  ++NK +IG +G LK LI LL+EG+P+
Sbjct: 121 ENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEGNPL 180

Query: 307 VIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLSTNLKAIDE 366
            I+D A+AIF LC   EN+ +AV  GAV  + + IS G+ VDEL+++LA+L T+ KA++E
Sbjct: 181 AIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKAVEE 240

Query: 367 MCKLKTVPCMLRIMRKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQT 426
           + +L  V  +L+I R++  +  KEN   IL  IC +DR+KW EI+++ENA+GTI  LS+ 
Sbjct: 241 LGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKLSRE 300

Query: 427 GTSRARRKALGILEKLRRAAAITHTA 446
           GTSRA+RKA GIL++LR+A  +THTA
Sbjct: 301 GTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Spg017170 vs. TAIR 10
Match: AT5G18320.1 (ARM repeat superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 4.0e-65
Identity = 170/447 (38.03%), Postives = 264/447 (59.06%), Query Frame = 0

Query: 8   DLLKKDLKNLLDRIVVE---EDFDFSTA--------DEAIEKLTALKISKIAAASPESGE 67
           D L+++L+ +L  I+ +    D D + A        DEA+  LT L+  K+ +  PES  
Sbjct: 10  DTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLR--KVESKIPESDI 69

Query: 68  SSGSTTLVLPEMFLCPISGEFMKDPVLLQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDM 127
           S     + +P+ F+C +S   M +PV++ +GQTY++  I +W  K   TCPKTK+VLS  
Sbjct: 70  S----PVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWL-KHERTCPKTKQVLSHR 129

Query: 128 TLTPNLLLRSMMMQWYAENGLPIPDVIFEEAIAYADESRLDELLNKL-LSQSLSDQKEAA 187
              PN L+  ++ QW   N         +E +A    S ++ LL ++  S S++DQ EAA
Sbjct: 130 LWIPNHLISDLITQWCLVNKYD-HQKPSDELVAELFTSDIEALLQRVSSSSSVADQIEAA 189

Query: 188 KELRRIARLNHEFRALF-ANLPGSIERLLHPLSV--ENVNLHPDLQEDLITTVLNISIHD 247
           KELR   +     R  F A +  SI RLL PLS   E V+   +LQE+++T + N+SI +
Sbjct: 190 KELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSILE 249

Query: 248 ANKKYLAEEPLVLPLLIQSLQYGSIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILL 307
           +NK  +AE  LV+PLL +SL+ G+ E + NAAA + SL+  ++NK +IGN+ A+K LI L
Sbjct: 250 SNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALIDL 309

Query: 308 LDEGHPVVIRDAASAIFQLCTTLENKEKAVCSGAVGSIVRNISQGIMVDELVSVLALLST 367
           ++EG  +  ++A S +F LC  LENK K V +G + +  + I  G  VDEL+S+LAL+ST
Sbjct: 310 IEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIST 369

Query: 368 NLKAIDEMCKLKTVPCMLRIMRKTTSQGVKENCASILFIIC--TADRSKWTEIQDDENAN 427
           + +A++EM KL  +  +  I+RK +S    EN   I+F +     DRS+   + ++EN +
Sbjct: 370 HNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQH 429

Query: 428 GTIFDLSQTGTSRARRKALGILEKLRR 438
           GT   L++ G+ RA RKA GIL+ ++R
Sbjct: 430 GTFTKLAKQGSVRAARKAQGILQWIKR 448

BLAST of Spg017170 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 234.2 bits (596), Expect = 2.1e-61
Identity = 154/431 (35.73%), Postives = 247/431 (57.31%), Query Frame = 0

Query: 33  DEAIEKLTALKISKIAAASPESGESSGST---------TLVLPEMFLCPISGEFMKDPVL 92
           D+  E++++L  + +   + ES +   ST         + V+PE F CPIS E MKDPV+
Sbjct: 207 DDCFERMSSLLKNLVDFVTMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVI 266

Query: 93  LQTGQTYDRSSIEKWFHKGHNTCPKTKEVLSDMTLTPNLLLRSMMMQWYAENGLPIPD-- 152
           + TGQTY+RSSI+KW   GH TCPK++E L    LTPN +L+S++  W   NG+ +P   
Sbjct: 267 VSTGQTYERSSIQKWLDAGHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQ 326

Query: 153 ------VIFEEAIAYADESRLDELLNKLLSQSLSDQKEAAKELRRIARLNHEFRALFANL 212
                  I   + +  D + +  LL KL + +   Q+ AA ELR +A+ N + R   A  
Sbjct: 327 GSCRTTKIGGSSSSDCDRTFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAE- 386

Query: 213 PGSIERLLHPLSVENVNLHPDLQEDLITTVLNISIHDANKKYLAEEPLVLPLLIQSLQYG 272
            G+I  L+  LS  +    P  QE  +T +LN+SI++ NK  + +   +   +++ L+ G
Sbjct: 387 AGAIPLLVELLSSPD----PRTQEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNG 446

Query: 273 SIEVKANAAAAIFSLAFHEANKNMIGNAGALKHLILLLDEGHPVVIRDAASAIFQLCTTL 332
           S+E + NAAA +FSL+  + NK  IG AGA++ LI LL+EG     +DAA+AIF LC   
Sbjct: 447 SMEARENAAATLFSLSVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQ 506

Query: 333 ENKEKAVCSGAVGSIVRNISQ--GIMVDELVSVLALLSTNLKAIDEMCKLKTVPCMLRIM 392
            NK +AV  G V  + R +    G MVDE +++LA+LSTN +    + + +++P ++ I+
Sbjct: 507 GNKSRAVKGGIVDPLTRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEII 566

Query: 393 RKTTSQGVKENCASILFIICTADRSKWTEIQDDENANGTIFDLSQTGTSRARRKALGILE 445
           R T S   +EN A+IL+ +C  +  +   +  +  A+  + +L++ GT RA+RKA  +LE
Sbjct: 567 R-TGSPRNRENAAAILWYLCIGNIER-LNVAREVGADVALKELTENGTDRAKRKAASLLE 626

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022965721.11.1e-16066.89U-box domain-containing protein 9-like [Cucurbita maxima][more]
KAG7021008.15.3e-16066.59U-box domain-containing protein 9, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022937712.16.9e-16066.81U-box domain-containing protein 9-like [Cucurbita moschata][more]
XP_023538150.12.6e-15966.52U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo][more]
KAG6586176.14.5e-15966.01U-box domain-containing protein 9, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q9SRT01.1e-9946.73U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 S... [more]
Q3E9F75.6e-6438.03Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q8VZ402.9e-6035.73U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q8GUG94.9e-6038.24U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
Q5VRH91.4e-5936.51U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Match NameE-valueIdentityDescription
A0A6J1HMF95.1e-16166.89RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465532 P... [more]
A0A6J1FC003.3e-16066.81RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111444033... [more]
A0A6J1CT874.4e-15265.49RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101411... [more]
A0A5A7UZN61.5e-14964.25RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B4X71.5e-14964.25RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485858 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G07360.17.7e-10146.73plant U-box 9 [more]
AT3G07360.26.8e-7348.16plant U-box 9 [more]
AT3G07360.36.8e-7348.16plant U-box 9 [more]
AT5G18320.14.0e-6538.03ARM repeat superfamily protein [more]
AT3G54850.12.1e-6135.73plant U-box 14 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 273..313
e-value: 0.0019
score: 27.5
coord: 352..394
e-value: 21.0
score: 10.8
coord: 231..272
e-value: 9.0
score: 13.6
IPR000225ArmadilloPFAMPF00514Armcoord: 275..312
e-value: 1.2E-4
score: 22.0
IPR003613U box domainSMARTSM00504Ubox_2coord: 68..131
e-value: 4.7E-30
score: 115.8
IPR003613U box domainPFAMPF04564U-boxcoord: 66..135
e-value: 1.6E-18
score: 66.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 64..138
score: 34.270008
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 65..137
e-value: 1.8E-25
score: 90.8
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 141..441
e-value: 1.4E-31
score: 111.4
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 8..443
NoneNo IPR availablePANTHERPTHR23315:SF253U-BOX DOMAIN-CONTAINING PROTEIN 9coord: 8..443
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 69..111
e-value: 2.67886E-23
score: 89.9854
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 53..141
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 156..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg017170.1Spg017170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity