Spg017019 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg017019
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Alpha-amylase
Location	scaffold9: 45794925 .. 45796863 (+)
RNA-Seq Expression	Spg017019
Synteny	Spg017019

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGCTTATAAAAGCAAAACCACTCTGCTCATTTCCAAAACAATGGGGTTTCTTGCTTCTCTGTGTTTCCTCTACCTCTCTATTTCCCTCTTCCCATCCCCCTCTTCTTCCACCTTACTCTTTCAGGTAAACAAATCCACTTTACTCTTCAATATTTTTCAAGCATTTGCATTAATGTAAACTCACTTTTAGAATCTTGAATTGATGTAAACAAACAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTGAAGAACATAATCCCTGACCTTGTAAATGCTAAAGTCACTCATGTATGGCTTCCTCCACCCTCCCAATCAGCTTCTTCACAAGGCAAGCTTCTTTCTCTGTTCCTTCAGCTCATTATCAAGAACATTTCACTAAATTTGTGTTGCTCAAAACCCTTTACACAGGATACTTACCCGGAAGGCTGTATGATCTGGATGCATCAAAATATGGAACCCAGAATGAACTCAAGTCTCTGATATCCGCCCTCCATGAGAAGGGAATCAAAGCCGTCGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAGGACCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGATGGCCGCCTTGATTGGGGTCCTTCCTTCATTTGCAGCGATGACACTGATTATTCCGATGGAACGGGCAATCGAGACAGTGGTGAACCCTATCAACCAGCACCTGACATCGACCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGCATTACAAAGCTCTACATGGATCAAACCTCACCAGATTTCGCAGTCGGTGAAAAATGGGATTCACTTTCTTATGGACAGGATGGAAAACCTAATCAGAACCAAGACGCACATCGCGATGCTCTTGCAGATTGGGTTAGGGCTTCGGGCGACACTGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAAGGGGAGCTATGGAGGTTGAAGGATTCTAAAGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACTTTCATCGACAATCACGACACCGGTTCAACTCAAAGAATGTGGCCATTCCCTTCCGATAAAGTGATCCAGGGTTACGCATATATTCTCACTCATCCTGGAACGCCTTCCATTGTAATTCATTCAACAATACGGTTTAGATTTTTGCGAATCGAAACTTAATTACCTGCAATTCACGTTCTGTTTGTTTGTTTGTTGCAGTTCTACGATCACTTCGTTGATTGGGGATTGAAGGAGGAGATAAGCAAATTGAGTGAAATTAGAGCAAGGAATGGGATCGGGAAAAAGAGCACGGTGAATATTATGGCTTCCGACGGCGGACTGTACGTTGCTGGCGTTGATGGGAAGATTATAATGAAGATTGGGCCGAACATGGATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCCACTTCTGGAATGGAGTATGCTGTGTGGGAGAAAAACTGAATTCGTGAATGGGTTCCTTGAAAGTCCGCCATTGATGGAATAAAAGTATTTAAAACCAAAAAATTCAAAAATATATACTAAATTAATAAATAAATATTTAATATTTTTGAAATGTGTTAAATATATATATATATATATTTGAAAATGTGTTAAAATACTAATTATTAATATTATCTCCATATTAAGTATATTTCGTTACCATTTTGGCATTTTCTTTATATTTTTATTGATGAGCAGTGTGGAAGAGACCATCTAAAAGACGATTCTAATGTTATAACTAAAGACATGCCCTTTTTTTTTTTTAAGGCAGGAATAATTATATGCTAAATATACACATGTATATTTATATTTTGAGATGAGACA

mRNA sequence

ATGAAAGCTTATAAAAGCAAAACCACTCTGCTCATTTCCAAAACAATGGGGTTTCTTGCTTCTCTGTGTTTCCTCTACCTCTCTATTTCCCTCTTCCCATCCCCCTCTTCTTCCACCTTACTCTTTCAGGTAAACAAATCCACTTTACTCTTCAATATTTTTCAAGCATTTGCATTAATTCACTCATGTATGGCTTCCTCCACCCTCCCAATCAGCTTCTTCACAAGGCAAGCTTCTTTCTCTGTTCCTTCAGCTCATTATCAAGAACATTTCACTAAATTTGTGTTGCTCAAAACCCTTTACACAGGATACTTACCCGGAAGGCTGTATGATCTGGATGCATCAAAATATGGAACCCAGAATGAACTCAAGTCTCTGATATCCGCCCTCCATGAGAAGGGAATCAAAGCCGTCGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAGGACCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGATGGCCGCCTTGATTGGGGTCCTTCCTTCATTTGCAGCGATGACACTGATTATTCCGATGGAACGGGCAATCGAGACAGTGGTGAACCCTATCAACCAGCACCTGACATCGACCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGCATTACAAAGCTCTACATGGATCAAACCTCACCAGATTTCGCAGTCGGTGAAAAATGGGATTCACTTTCTTATGGACAGGATGGAAAACCTAATCAGAACCAAGACGCACATCGCGATGCTCTTGCAGATTGGGTTAGGGCTTCGGGCGACACTGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAAGGGGAGCTATGGAGGTTGAAGGATTCTAAAGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACTTTCATCGACAATCACGACACCGGTTCAACTCAAAGAATGTGGCCATTCCCTTCCGATAAAGTGATCCAGGGTTACGCATATATTCTCACTCATCCTGGAACGCCTTCCATTTTCTACGATCACTTCGTTGATTGGGGATTGAAGGAGGAGATAAGCAAATTGAGTGAAATTAGAGCAAGGAATGGGATCGGGAAAAAGAGCACGGTGAATATTATGGCTTCCGACGGCGGACTGTACGTTGCTGGCGTTGATGGGAAGATTATAATGAAGATTGGGCCGAACATGGATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCCACTTCTGGAATGGAGTATGCTGTGTGGGAGAAAAACTGA

Coding sequence (CDS)

ATGAAAGCTTATAAAAGCAAAACCACTCTGCTCATTTCCAAAACAATGGGGTTTCTTGCTTCTCTGTGTTTCCTCTACCTCTCTATTTCCCTCTTCCCATCCCCCTCTTCTTCCACCTTACTCTTTCAGGTAAACAAATCCACTTTACTCTTCAATATTTTTCAAGCATTTGCATTAATTCACTCATGTATGGCTTCCTCCACCCTCCCAATCAGCTTCTTCACAAGGCAAGCTTCTTTCTCTGTTCCTTCAGCTCATTATCAAGAACATTTCACTAAATTTGTGTTGCTCAAAACCCTTTACACAGGATACTTACCCGGAAGGCTGTATGATCTGGATGCATCAAAATATGGAACCCAGAATGAACTCAAGTCTCTGATATCCGCCCTCCATGAGAAGGGAATCAAAGCCGTCGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAGGACCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTCCAGATGGCCGCCTTGATTGGGGTCCTTCCTTCATTTGCAGCGATGACACTGATTATTCCGATGGAACGGGCAATCGAGACAGTGGTGAACCCTATCAACCAGCACCTGACATCGACCATCTCAATCCACAAGTTCAGAAAGAAATATCAGAATGGATGAGATGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGCATTACAAAGCTCTACATGGATCAAACCTCACCAGATTTCGCAGTCGGTGAAAAATGGGATTCACTTTCTTATGGACAGGATGGAAAACCTAATCAGAACCAAGACGCACATCGCGATGCTCTTGCAGATTGGGTTAGGGCTTCGGGCGACACTGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAAGGGGAGCTATGGAGGTTGAAGGATTCTAAAGGGAAGCCGCCGGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACTTTCATCGACAATCACGACACCGGTTCAACTCAAAGAATGTGGCCATTCCCTTCCGATAAAGTGATCCAGGGTTACGCATATATTCTCACTCATCCTGGAACGCCTTCCATTTTCTACGATCACTTCGTTGATTGGGGATTGAAGGAGGAGATAAGCAAATTGAGTGAAATTAGAGCAAGGAATGGGATCGGGAAAAAGAGCACGGTGAATATTATGGCTTCCGACGGCGGACTGTACGTTGCTGGCGTTGATGGGAAGATTATAATGAAGATTGGGCCGAACATGGATCTTGGAAATCTGATTCCGTCGGATTATCAGCTTGCCACTTCTGGAATGGAGTATGCTGTGTGGGAGAAAAACTGA

Protein sequence

MKAYKSKTTLLISKTMGFLASLCFLYLSISLFPSPSSSTLLFQVNKSTLLFNIFQAFALIHSCMASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEKN

Homology

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BLAST of Spg017019 vs. NCBI nr
Match: XP_022142476.1 (alpha-amylase [Momordica charantia])

HSP 1 Score: 712.2 bits (1837), Expect = 2.8e-201
Identity = 355/455 (78.02%), Postives = 383/455 (84.18%), Query Frame = 0


Query: 1   MKAYKSKTTLLISKTMGFLASLCFLYLSISLFPSPSSSTLLFQ-VN-KSTLLFNIFQAFA 60
           M   K+K  L ISKTMGFLASLCFLYLS+SLFPSPSSS LLFQ  N +S+     + +  
Sbjct: 1   MMLIKAKYALPISKTMGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLK 60

Query: 61  LIHSCMASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYG 120
            I   + ++ +   +    +    P                   GYLPGRLYDLDASKYG
Sbjct: 61  NIIPDLVNARITHVWLPPPSQSVSPQ------------------GYLPGRLYDLDASKYG 120

Query: 121 TQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICS 180
           TQNELKSLI+ALHEKGIKA+ADIVINHRTAEKQDQRGIWCIFEGGTPD RLDWGPSFICS
Sbjct: 121 TQNELKSLIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICS 180

Query: 181 DDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYA 240
           DDTDYSDG GN DSGEPY PAPDIDHL PQVQKEISEWMRWLKTEIGFDGWRFDFVKGYA
Sbjct: 181 DDTDYSDGKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYA 240

Query: 241 PSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFT 300
           PS+TKLYMDQTSPDFAVGEKWDSLS G DGK NQNQDAHRD+LA+WV+ASGD VTAFDFT
Sbjct: 241 PSVTKLYMDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFT 300

Query: 301 TKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQG 360
           TKGILQAAVQGELWRLKDS GKPPGFIGIKPESAVTF+DNHDTGSTQ++WPFPSDKVIQG
Sbjct: 301 TKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQG 360

Query: 361 YAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVD 420
           YAYILTHPGTP+IFYDHFV+WGLKEEI +LSEIRARN IGK S VNI+ASD  LYVAGVD
Sbjct: 361 YAYILTHPGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVD 420

Query: 421 GKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           GKIIMKIG N +LGNLIPS++QLATSGMEYAVWEK
Sbjct: 421 GKIIMKIGANTNLGNLIPSEFQLATSGMEYAVWEK 437

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BLAST of Spg017019 vs. NCBI nr
Match: XP_038896126.1 (alpha-amylase-like [Benincasa hispida])

HSP 1 Score: 708.4 bits (1827), Expect = 4.1e-200
Identity = 354/441 (80.27%), Postives = 371/441 (84.13%), Query Frame = 0


Query: 16  MGFLASLCFLYLSISLFPSPSSSTLLFQVNKSTLLFNIFQAFALIHSCMASSTLPISFFT 75
           MGFLASLCFLYL +SLFPSPSSS        STLLF  F   +       +S   I    
Sbjct: 1   MGFLASLCFLYLCVSLFPSPSSS--------STLLFQGFNWESSSKGGWYNSLNNI---- 60

Query: 76  RQASFSVP---SAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYGTQNELKSLISALHE 135
                 +P   SA     +           GYLPGRLYDL+ASKYGTQNELKSLI+ LHE
Sbjct: 61  ------IPDLLSAEVTHVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHE 120

Query: 136 KGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNRDS 195
           KGIKA+ADIVINHRTAEKQDQRGIWCIFEGGTPD RLDWGPSFICSDDT+YSDG GN DS
Sbjct: 121 KGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDS 180

Query: 196 GEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPD 255
           GEPY PAPDIDHLNPQVQKE+SEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSP+
Sbjct: 181 GEPYPPAPDIDHLNPQVQKELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPE 240

Query: 256 FAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFTTKGILQAAVQGELW 315
           FAVGEKWD LSYGQDGKPNQNQD+HRDALA+WVRASGD VTAFDFTTKGILQAAV+GELW
Sbjct: 241 FAVGEKWDGLSYGQDGKPNQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELW 300

Query: 316 RLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIF 375
           RLKDS G PPGFIGIKPESAVTFI+NHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIF
Sbjct: 301 RLKDSNGNPPGFIGIKPESAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIF 360

Query: 376 YDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMDLG 435
           YDHFVDWGLKEEI KLS IRARNGIGK STVNIMAS+G LYVAGVDGKIIMKIG N  LG
Sbjct: 361 YDHFVDWGLKEEIMKLSGIRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLG 420

Query: 436 NLIPSDYQLATSGMEYAVWEK 454
           NLIPSDYQLAT GM+YAVWEK
Sbjct: 421 NLIPSDYQLATYGMDYAVWEK 423

[Close]

BLAST of Spg017019 vs. NCBI nr
Match: XP_022936258.1 (alpha-amylase, partial [Cucurbita moschata])

HSP 1 Score: 690.3 bits (1780), Expect = 1.1e-194
Identity = 348/450 (77.33%), Postives = 373/450 (82.89%), Query Frame = 0


Query: 15  TMGFLASLCFL-YLSISLFPSPS-SSTLLFQ------VNKS---TLLFNIFQAFALIHSC 74
           TMGFLASLCFL YLS+ LFPSPS SSTLLFQ       +K      L NI     L+H+ 
Sbjct: 24  TMGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIP--DLVHAK 83

Query: 75  MASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYGTQNEL 134
           +    LP            PS                  GYLPGRLYDL+ASKYGTQNEL
Sbjct: 84  VTHVWLP-----------PPSQSASPQ------------GYLPGRLYDLNASKYGTQNEL 143

Query: 135 KSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDY 194
           KSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+Y
Sbjct: 144 KSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEY 203

Query: 195 SDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITK 254
           SDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITK
Sbjct: 204 SDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITK 263

Query: 255 LYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFTTKGIL 314
           LYMDQTSPDFAVGEKWDSLS G DGKP+ NQD HR+ALA+WVR SGDTVTAFDFTTKGIL
Sbjct: 264 LYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGIL 323

Query: 315 QAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYIL 374
           QAAVQGELWRLKDS GKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYIL
Sbjct: 324 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYIL 383

Query: 375 THPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIM 434
           THPGTPSIFYDHFV+WGLKEE+ KLS IRARNGIG++STVNIMASDG LYVAG+DGKIIM
Sbjct: 384 THPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIM 443

Query: 435 KIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           KIGPN +LGNL+PSDY+LA SGM+YAVWEK
Sbjct: 444 KIGPNTNLGNLVPSDYELAASGMQYAVWEK 448

[Close]

BLAST of Spg017019 vs. NCBI nr
Match: XP_023536126.1 (alpha-amylase, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 688.0 bits (1774), Expect = 5.7e-194
Identity = 351/464 (75.65%), Postives = 379/464 (81.68%), Query Frame = 0


Query: 2   KAYKSK-TTLLISKTMGFLASLCFL-YLSISLFPSPS-SSTLLFQ------VNKS---TL 61
           +AYK +  T    +TMGFLASLCFL YLS+ +FPSPS SSTLLFQ       +K      
Sbjct: 28  EAYKCRIPTAHFPQTMGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNS 87

Query: 62  LFNIFQAFALIHSCMASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRL 121
           L NI     L+H+ +    LP            PS                  GYLPGRL
Sbjct: 88  LNNIIP--DLVHAKVTHVWLP-----------PPSQSASPQ------------GYLPGRL 147

Query: 122 YDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRL 181
           YDL+ASKYGTQ+ELKSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RL
Sbjct: 148 YDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRL 207

Query: 182 DWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGW 241
           DWGPSFICSDDT+YSDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGW
Sbjct: 208 DWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGW 267

Query: 242 RFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASG 301
           RFDFVKGYAP ITKLYMDQTSPDFAVGEKWDSLS G DGKP+ NQD HR+ALA+WVR SG
Sbjct: 268 RFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSG 327

Query: 302 DTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWP 361
           DTVTAFDFTTKGILQAAVQGELWRLKDS GKPPGFIGIKPESAVTFIDNHDTGSTQ++WP
Sbjct: 328 DTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWP 387

Query: 362 FPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASD 421
           FPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE+ KLS IR RNGIG++STVNIMASD
Sbjct: 388 FPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASD 447

Query: 422 GGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           G LYVAG+DGKIIMKIGPN +LGNLIPSDY LATSGM+YAVWEK
Sbjct: 448 GDLYVAGIDGKIIMKIGPNTNLGNLIPSDYVLATSGMQYAVWEK 466

[Close]

BLAST of Spg017019 vs. NCBI nr
Match: XP_022976296.1 (alpha-amylase [Cucurbita maxima])

HSP 1 Score: 684.1 bits (1764), Expect = 8.2e-193
Identity = 345/449 (76.84%), Postives = 371/449 (82.63%), Query Frame = 0


Query: 16  MGFLASLCFL-YLSISLFPSPS-SSTLLFQ------VNKS---TLLFNIFQAFALIHSCM 75
           MGFLASLCFL YLS+ +FPSPS SSTLLFQ       +K      L NI     L+H+ +
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIP--DLVHAKV 60

Query: 76  ASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYGTQNELK 135
               LP            PS                  GYLPGRLYDL+ASKYGTQ+ELK
Sbjct: 61  THVWLP-----------PPSQSASPQ------------GYLPGRLYDLNASKYGTQDELK 120

Query: 136 SLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYS 195
           SLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+YS
Sbjct: 121 SLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYS 180

Query: 196 DGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKL 255
           DG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKL
Sbjct: 181 DGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKL 240

Query: 256 YMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFTTKGILQ 315
           YMDQTSPDFAVGEKWDSLS G DGKP+ NQD HR+ALA+WVR SGDTVTAFDFTTKGILQ
Sbjct: 241 YMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQ 300

Query: 316 AAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILT 375
           AAVQGELWRLKD  GKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILT
Sbjct: 301 AAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILT 360

Query: 376 HPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMK 435
           HPGTPSIFYDHFV+WGLKEE+ KLS IR RNGIG++STVNIMASDG LYVAG+DGKIIMK
Sbjct: 361 HPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMK 420

Query: 436 IGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           IGPN +LGNLIPSDY+LATSGM+YAVWEK
Sbjct: 421 IGPNTNLGNLIPSDYELATSGMQYAVWEK 424

[Close]

BLAST of Spg017019 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 3.8e-177
Identity = 283/350 (80.86%), Postives = 315/350 (90.00%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GYLPGRLYDLDASKYG++NELKSLI+A HEKGIK +ADIVINHRTAE++D RGI+CIFEG
Sbjct: 72  GYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEG 131

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
           GTPD R DWGPSFIC DDT YSDGTGN DSGE Y  APDIDHLNPQVQ+E+SEWM WLKT
Sbjct: 132 GTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKT 191

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           EIGFDGWRFDFVKGYAPSI+K+YM+QT PDFAVGEKWDS+SYGQDGKPN NQD+HR AL 
Sbjct: 192 EIGFDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALV 251

Query: 283 DWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTG 342
           +WV ++G  +TAFDFTTKGILQAAVQGELWRL D  GKPPG IG+KPE+AVTFIDNHDTG
Sbjct: 252 NWVESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTG 311

Query: 343 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKST 402
           STQR+WPFPSDKV+QGYAYILTHPGTPSIFYDHF DWGLKE+I+KLS IR RNGI +KST
Sbjct: 312 STQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKST 371

Query: 403 VNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWE 453
           V IMAS+G LYVA +D KI++KIGP MDLGNLIPS+  +ATSG +YAVWE
Sbjct: 372 VKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVWE 421

[Close]

BLAST of Spg017019 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.1e-144
Identity = 223/351 (63.53%), Postives = 278/351 (79.20%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GY+PGRLYDLDASKYGT+ ELKSLI A H+K ++ +ADIVINHR A+ +D RG++C+FEG
Sbjct: 75  GYMPGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEG 134

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
           GTPDGRLDWGP  ICSDDT YS+G G+RD+G  +  APDIDHLNP+VQ+E+++W+ WL+T
Sbjct: 135 GTPDGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRT 194

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           ++GFDGWR DF KGY+  + ++Y+D T+P F VGE W SL Y  DGKP+ NQDA R  L 
Sbjct: 195 DLGFDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELV 254

Query: 283 DWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTG 342
           +WV   G   TAFDFTTKGILQAAVQGELWRL D  GK PG +G  P+ AVTF+DNHDTG
Sbjct: 255 NWVEGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTG 314

Query: 343 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKST 402
           STQ +WPFPSDKV+QGYAYILTHPG P IFYDH  DW L+ EI+ L+EIR+RNGI  +ST
Sbjct: 315 STQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAEST 374

Query: 403 VNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           ++I+ ++G +YVA +DGK+I K+GP  D G +IPSD+ +   G +Y VWEK
Sbjct: 375 LDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEK 425

[Close]

BLAST of Spg017019 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 514.2 bits (1323), Expect = 1.5e-144
Identity = 231/353 (65.44%), Postives = 276/353 (78.19%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GY+PGRLYDLDASKYG + +LKSLI ALH KG+KA+ADIVINHRTAE +D RGI+CIFEG
Sbjct: 74  GYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYCIFEG 133

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
           GTPD RLDWGP  IC DD  Y+DGTGN D+G  +  APDIDHLN +VQKE+ EW+ WLK 
Sbjct: 134 GTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKELVEWLNWLKA 193

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           +IGFDGWRFDF KGY+  + K+Y+D++ P FAV E W SL+YG DGKPN NQD HR  L 
Sbjct: 194 DIGFDGWRFDFAKGYSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHRQELV 253

Query: 283 DWVRASG--DTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHD 342
           +WV   G     T FDFTTKGIL  AV+GELWRL+ + GK PG IG  P  AVTF+DNHD
Sbjct: 254 NWVDKVGGKGPATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHD 313

Query: 343 TGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKK 402
           TGSTQ MWPFPSD+V+QGYAYILTHPGTP IFYDHF DWGLKEEI +L  +R R+GI  +
Sbjct: 314 TGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRTRHGIHNE 373

Query: 403 STVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           S + I+ +D  LY+A +DGK+I+K+GP  D+GNLIP  +++A  G +YAVWEK
Sbjct: 374 SKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVWEK 426

[Close]

BLAST of Spg017019 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 505.0 bits (1299), Expect = 8.9e-142
Identity = 223/351 (63.53%), Postives = 270/351 (76.92%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GY+PGRLYDLDASKYGT  ELKSLI+A H KG++ VAD+VINHR AEK+D RG++C+FEG
Sbjct: 75  GYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEG 134

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
           GTPD RLDWGP  ICSDDT YSDGTG+RD+GE +  APDIDHLNP+VQ+E+++W+ WLK+
Sbjct: 135 GTPDDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPRVQRELTDWLNWLKS 194

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           ++GFDGWR DF KGY+  I K+Y++   P F V E W+SLSY  DGKP  NQD  R  L 
Sbjct: 195 DVGFDGWRLDFAKGYSTDIAKMYVESCKPGFVVAEIWNSLSYNGDGKPAANQDQGRQELV 254

Query: 283 DWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTG 342
           +WV A G     FDFTTKG+LQA VQGELWRL+D  GK  G IG  PE AVTF+DNHDTG
Sbjct: 255 NWVNAVGGPAMTFDFTTKGLLQAGVQGELWRLRDGNGKAAGMIGWLPEKAVTFVDNHDTG 314

Query: 343 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKST 402
           STQ++WPFPSDKV+QGYAYILTHPG P IFYDH  DW LK+EI+ L+ IR RNGI   S 
Sbjct: 315 STQKLWPFPSDKVMQGYAYILTHPGVPCIFYDHMFDWNLKQEITALAAIRERNGINAGSK 374

Query: 403 VNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           + I+ +D   YVA VD K+++KIG   D+GN +PSD+     G +Y+VWEK
Sbjct: 375 LRIVVADADAYVAVVDEKVMVKIGTRYDVGNAVPSDFHQTVHGKDYSVWEK 425

[Close]

BLAST of Spg017019 vs. ExPASy Swiss-Prot
Match: Q8VZ56 (Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 3.4e-141
Identity = 233/352 (66.19%), Postives = 284/352 (80.68%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GYLPG+LYDL++SKYG++ ELKSLI AL++KGIKA+ADIVINHRTAE++D +  +C FEG
Sbjct: 75  GYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEG 134

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
           GT D RLDW PSF+C +D  +  GTGN D+G  +  APDIDHLNP+VQKE+SEWM WLKT
Sbjct: 135 GTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKT 194

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           EIGF GWRFD+V+GYA SITKLY+  TSPDFAVGEKWD + YG DGK + +Q+ HR  L 
Sbjct: 195 EIGFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLK 254

Query: 283 DWV-RASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDT 342
            W+  A G  +TAFDFTTKGILQ+AV+GELWRLKDS+GKPPG IGI P +AVTFIDNHD 
Sbjct: 255 QWIEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD- 314

Query: 343 GSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKS 402
             T R W FPSDKV+ GY YILTHPGTP IFY+H+++WGLKE ISKL  IR +NGIG  S
Sbjct: 315 --TFRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTS 374

Query: 403 TVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           +V I A++  LY+A +D K+IMKIGP  D+G L+PS++ LA SG+++AVWEK
Sbjct: 375 SVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEK 422

[Close]

BLAST of Spg017019 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.4e-201
Identity = 355/455 (78.02%), Postives = 383/455 (84.18%), Query Frame = 0


Query: 1   MKAYKSKTTLLISKTMGFLASLCFLYLSISLFPSPSSSTLLFQ-VN-KSTLLFNIFQAFA 60
           M   K+K  L ISKTMGFLASLCFLYLS+SLFPSPSSS LLFQ  N +S+     + +  
Sbjct: 1   MMLIKAKYALPISKTMGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLK 60

Query: 61  LIHSCMASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYG 120
            I   + ++ +   +    +    P                   GYLPGRLYDLDASKYG
Sbjct: 61  NIIPDLVNARITHVWLPPPSQSVSPQ------------------GYLPGRLYDLDASKYG 120

Query: 121 TQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICS 180
           TQNELKSLI+ALHEKGIKA+ADIVINHRTAEKQDQRGIWCIFEGGTPD RLDWGPSFICS
Sbjct: 121 TQNELKSLIAALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDNRLDWGPSFICS 180

Query: 181 DDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYA 240
           DDTDYSDG GN DSGEPY PAPDIDHL PQVQKEISEWMRWLKTEIGFDGWRFDFVKGYA
Sbjct: 181 DDTDYSDGKGNPDSGEPYPPAPDIDHLKPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYA 240

Query: 241 PSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFT 300
           PS+TKLYMDQTSPDFAVGEKWDSLS G DGK NQNQDAHRD+LA+WV+ASGD VTAFDFT
Sbjct: 241 PSVTKLYMDQTSPDFAVGEKWDSLSDGPDGKLNQNQDAHRDSLANWVKASGDAVTAFDFT 300

Query: 301 TKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQG 360
           TKGILQAAVQGELWRLKDS GKPPGFIGIKPESAVTF+DNHDTGSTQ++WPFPSDKVIQG
Sbjct: 301 TKGILQAAVQGELWRLKDSNGKPPGFIGIKPESAVTFVDNHDTGSTQKLWPFPSDKVIQG 360

Query: 361 YAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVD 420
           YAYILTHPGTP+IFYDHFV+WGLKEEI +LSEIRARN IGK S VNI+ASD  LYVAGVD
Sbjct: 361 YAYILTHPGTPAIFYDHFVEWGLKEEIIRLSEIRARNRIGKTSAVNILASDADLYVAGVD 420

Query: 421 GKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           GKIIMKIG N +LGNLIPS++QLATSGMEYAVWEK
Sbjct: 421 GKIIMKIGANTNLGNLIPSEFQLATSGMEYAVWEK 437

[Close]

BLAST of Spg017019 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 5.5e-195
Identity = 348/450 (77.33%), Postives = 373/450 (82.89%), Query Frame = 0


Query: 15  TMGFLASLCFL-YLSISLFPSPS-SSTLLFQ------VNKS---TLLFNIFQAFALIHSC 74
           TMGFLASLCFL YLS+ LFPSPS SSTLLFQ       +K      L NI     L+H+ 
Sbjct: 24  TMGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIP--DLVHAK 83

Query: 75  MASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYGTQNEL 134
           +    LP            PS                  GYLPGRLYDL+ASKYGTQNEL
Sbjct: 84  VTHVWLP-----------PPSQSASPQ------------GYLPGRLYDLNASKYGTQNEL 143

Query: 135 KSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDY 194
           KSLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+Y
Sbjct: 144 KSLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEY 203

Query: 195 SDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITK 254
           SDG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITK
Sbjct: 204 SDGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITK 263

Query: 255 LYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFTTKGIL 314
           LYMDQTSPDFAVGEKWDSLS G DGKP+ NQD HR+ALA+WVR SGDTVTAFDFTTKGIL
Sbjct: 264 LYMDQTSPDFAVGEKWDSLSSGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGIL 323

Query: 315 QAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYIL 374
           QAAVQGELWRLKDS GKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYIL
Sbjct: 324 QAAVQGELWRLKDSNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYIL 383

Query: 375 THPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIM 434
           THPGTPSIFYDHFV+WGLKEE+ KLS IRARNGIG++STVNIMASDG LYVAG+DGKIIM
Sbjct: 384 THPGTPSIFYDHFVEWGLKEELIKLSAIRARNGIGERSTVNIMASDGDLYVAGIDGKIIM 443

Query: 435 KIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           KIGPN +LGNL+PSDY+LA SGM+YAVWEK
Sbjct: 444 KIGPNTNLGNLVPSDYELAASGMQYAVWEK 448

[Close]

BLAST of Spg017019 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 4.0e-193
Identity = 345/449 (76.84%), Postives = 371/449 (82.63%), Query Frame = 0


Query: 16  MGFLASLCFL-YLSISLFPSPS-SSTLLFQ------VNKS---TLLFNIFQAFALIHSCM 75
           MGFLASLCFL YLS+ +FPSPS SSTLLFQ       +K      L NI     L+H+ +
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIP--DLVHAKV 60

Query: 76  ASSTLPISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYGTQNELK 135
               LP            PS                  GYLPGRLYDL+ASKYGTQ+ELK
Sbjct: 61  THVWLP-----------PPSQSASPQ------------GYLPGRLYDLNASKYGTQDELK 120

Query: 136 SLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYS 195
           SLI+ALH  GIKAVADIVINHRTA+KQD+RGIWCIFEGGTPD RLDWGPSFICSDDT+YS
Sbjct: 121 SLIAALHNNGIKAVADIVINHRTADKQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYS 180

Query: 196 DGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKL 255
           DG GN DSGEPY  APDIDHLNPQVQ E+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKL
Sbjct: 181 DGKGNPDSGEPYSAAPDIDHLNPQVQNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKL 240

Query: 256 YMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFTTKGILQ 315
           YMDQTSPDFAVGEKWDSLS G DGKP+ NQD HR+ALA+WVR SGDTVTAFDFTTKGILQ
Sbjct: 241 YMDQTSPDFAVGEKWDSLSPGPDGKPDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQ 300

Query: 316 AAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILT 375
           AAVQGELWRLKD  GKPPGFIGIKPESAVTFIDNHDTGSTQ++WPFPSDKVIQGYAYILT
Sbjct: 301 AAVQGELWRLKDPNGKPPGFIGIKPESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILT 360

Query: 376 HPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMK 435
           HPGTPSIFYDHFV+WGLKEE+ KLS IR RNGIG++STVNIMASDG LYVAG+DGKIIMK
Sbjct: 361 HPGTPSIFYDHFVEWGLKEELIKLSAIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMK 420

Query: 436 IGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           IGPN +LGNLIPSDY+LATSGM+YAVWEK
Sbjct: 421 IGPNTNLGNLIPSDYELATSGMQYAVWEK 424

[Close]

BLAST of Spg017019 vs. ExPASy TrEMBL
Match: A0A6A1W532 (Alpha-amylase OS=Morella rubra OX=262757 GN=CJ030_MR3G003095 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.4e-177
Identity = 289/351 (82.34%), Postives = 319/351 (90.88%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GY+PGRLYDLDASKYG+++ELKSLISALHEKGIKA+ADIVINHRTAEKQD RGIWCIFEG
Sbjct: 102 GYMPGRLYDLDASKYGSKDELKSLISALHEKGIKAIADIVINHRTAEKQDGRGIWCIFEG 161

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
           GTPD RLDWGPSFIC DDTDYSDG+GN DSGE ++PAPDIDHLNPQVQKE+S+WM WLKT
Sbjct: 162 GTPDARLDWGPSFICKDDTDYSDGSGNLDSGEDFRPAPDIDHLNPQVQKELSDWMNWLKT 221

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           EIGF GWRFDFVKGYAPSITK+YM+QT+PDFAVGE W+SL+Y   GKPN NQDAHR ALA
Sbjct: 222 EIGFSGWRFDFVKGYAPSITKIYMEQTTPDFAVGENWNSLAYDDGGKPNPNQDAHRGALA 281

Query: 283 DWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTG 342
           +WV+ +G  VTAFDFTTKGILQAAV+GELWRLKDS GKPPG IG KPESAVTFIDNHDTG
Sbjct: 282 NWVQGAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGTKPESAVTFIDNHDTG 341

Query: 343 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKST 402
           STQ++WPFPSDKV+QGYAYILTHPG P+IFYDHF DWGLKEEISKLS IR+RNGI   S 
Sbjct: 342 STQKLWPFPSDKVMQGYAYILTHPGIPTIFYDHFFDWGLKEEISKLSAIRSRNGISSTSR 401

Query: 403 VNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           VNI+ASD  LY+A VD KIIMKIGP MDLGNL+PS+YQ+ATSG +YAVWEK
Sbjct: 402 VNILASDADLYLAEVDNKIIMKIGPKMDLGNLVPSNYQVATSGNDYAVWEK 452

[Close]

BLAST of Spg017019 vs. ExPASy TrEMBL
Match: A0A2I4EXA7 (Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.4e-174
Identity = 307/443 (69.30%), Postives = 348/443 (78.56%), Query Frame = 0


Query: 16  MGFLASLCFLYLSISLFPSPSSSTLLFQ-----VNKSTLLFNIFQAFALIHSCMASSTLP 75
           M     LCFL L ISL  S ++ +LLFQ      +K    +N  + F      +A++ + 
Sbjct: 1   MNLKTFLCFLCLFISLCHSFAAPSLLFQGFNWESSKKGGWYNSLKNFV---PDLANAEIT 60

Query: 76  ISFFTRQASFSVPSAHYQEHFTKFVLLKTLYTGYLPGRLYDLDASKYGTQNELKSLISAL 135
             +    +  + P                   GYLPGRLYDLD SKYG+++ELK+LI+AL
Sbjct: 61  HVWLPPPSQSAAPE------------------GYLPGRLYDLDVSKYGSKDELKALIAAL 120

Query: 136 HEKGIKAVADIVINHRTAEKQDQRGIWCIFEGGTPDGRLDWGPSFICSDDTDYSDGTGNR 195
           HEKGIK VADIVINHRTAEKQD RGIW IFEGGTPD RLDWGPSFIC DD  YSDGTGN 
Sbjct: 121 HEKGIKVVADIVINHRTAEKQDDRGIWSIFEGGTPDARLDWGPSFICKDDIAYSDGTGNP 180

Query: 196 DSGEPYQPAPDIDHLNPQVQKEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTS 255
           DSGE Y PAPDIDH NPQVQKE+SEWM WLKTEIG+DGWRFDFVKGYAPSI K YM+QT 
Sbjct: 181 DSGEGYDPAPDIDHRNPQVQKELSEWMNWLKTEIGYDGWRFDFVKGYAPSIMKNYMEQTK 240

Query: 256 PDFAVGEKWDSLSYGQDGKPNQNQDAHRDALADWVRASGDTVTAFDFTTKGILQAAVQGE 315
           PDFAVGE W+SL+YG DGKPN NQDAHR ALA+WV ++G  VTAFDFTTKGILQAAV+GE
Sbjct: 241 PDFAVGENWNSLAYGDDGKPNPNQDAHRGALAEWVGSAGGAVTAFDFTTKGILQAAVEGE 300

Query: 316 LWRLKDSKGKPPGFIGIKPESAVTFIDNHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPS 375
           LWRLKDS GKPPG IGIKPESAVTFIDNHDTGSTQ +WPFPSDKV+QGYAYILTHPGTP+
Sbjct: 301 LWRLKDSNGKPPGLIGIKPESAVTFIDNHDTGSTQNIWPFPSDKVMQGYAYILTHPGTPT 360

Query: 376 IFYDHFVDWGLKEEISKLSEIRARNGIGKKSTVNIMASDGGLYVAGVDGKIIMKIGPNMD 435
           IFYDHF DWGLKEEISKL+ IR+RNGI  +S V+I+ASDG LYVAG+D KIIMKIGP MD
Sbjct: 361 IFYDHFFDWGLKEEISKLTAIRSRNGINTRSIVSILASDGDLYVAGIDDKIIMKIGPKMD 420

Query: 436 LGNLIPSDYQLATSGMEYAVWEK 454
           LGNLIPS++Q++TSG +YAVWEK
Sbjct: 421 LGNLIPSNFQVSTSGKDYAVWEK 422

[Close]

BLAST of Spg017019 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 503.1 bits (1294), Expect = 2.4e-142
Identity = 233/352 (66.19%), Postives = 284/352 (80.68%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GYLPG+LYDL++SKYG++ ELKSLI AL++KGIKA+ADIVINHRTAE++D +  +C FEG
Sbjct: 75  GYLPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEG 134

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
           GT D RLDW PSF+C +D  +  GTGN D+G  +  APDIDHLNP+VQKE+SEWM WLKT
Sbjct: 135 GTSDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKT 194

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           EIGF GWRFD+V+GYA SITKLY+  TSPDFAVGEKWD + YG DGK + +Q+ HR  L 
Sbjct: 195 EIGFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLK 254

Query: 283 DWV-RASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDT 342
            W+  A G  +TAFDFTTKGILQ+AV+GELWRLKDS+GKPPG IGI P +AVTFIDNHD 
Sbjct: 255 QWIEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD- 314

Query: 343 GSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEISKLSEIRARNGIGKKS 402
             T R W FPSDKV+ GY YILTHPGTP IFY+H+++WGLKE ISKL  IR +NGIG  S
Sbjct: 315 --TFRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTS 374

Query: 403 TVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWEK 454
           +V I A++  LY+A +D K+IMKIGP  D+G L+PS++ LA SG+++AVWEK
Sbjct: 375 SVTIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEK 422

[Close]

BLAST of Spg017019 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 335.9 bits (860), Expect = 5.1e-92
Identity = 169/352 (48.01%), Postives = 224/352 (63.64%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GY+P  LY+L+ S+YGT +ELK  +   H+ GIK + D V+NHR A  ++Q G+W +F  
Sbjct: 544 GYMPKDLYNLN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLF-- 603

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
               GRL+W    + +DD  +  G GN+ SG+ +  AP+IDH    V+K+I EW+ W+  
Sbjct: 604 ---GGRLNWDDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMME 663

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
           E+G+DGWR DFV+G+     K YMD + P FAVGE WDSLSY   G+ + NQDAHR  + 
Sbjct: 664 EVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTY-GEMDYNQDAHRQRIV 723

Query: 283 DWVRASGDTVTAFDFTTKGILQAAVQG-ELWRLKDSKGKPPGFIGIKPESAVTFIDNHDT 342
           DW+ A+     AFD TTKGIL  A+Q  E WRL D KGKPPG +G  P  AVTFI+NHDT
Sbjct: 724 DWINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDT 783

Query: 343 GSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDH-FVDWGLKEEISKLSEIRARNGIGKK 402
           GSTQ  W FP  K +QGYAYILTHPGTP++F+DH F D+    EI+ L  +R R  +  +
Sbjct: 784 GSTQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDY--HSEIAALLSLRNRQKLHCR 843

Query: 403 STVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPSDYQLATSGMEYAVWE 453
           S VNI  S+  +Y A +D K+ MKIGP          ++ +A  G +Y VWE
Sbjct: 844 SEVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Spg017019 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 332.0 bits (850), Expect = 7.4e-91
Identity = 167/355 (47.04%), Postives = 228/355 (64.23%), Query Frame = 0


Query: 103 GYLPGRLYDLDASKYGTQNELKSLISALHEKGIKAVADIVINHRTAEKQDQRGIWCIFEG 162
           GYLP  LY L+ S YG+++ LKSL+  + +  ++A+ADIVINHR    +   G++  ++G
Sbjct: 73  GYLPQDLYSLN-SAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG 132

Query: 163 GTPDGRLDWGPSFICSDDTDYSDGTGNRDSGEPYQPAPDIDHLNPQVQKEISEWMRWLKT 222
            +    L W    +    T  + G GNR +G+ +   P++DH    V+K+I  W+RWL+ 
Sbjct: 133 IS----LPWDEHAV----TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRN 192

Query: 223 EIGFDGWRFDFVKGYAPSITKLYMDQTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRDALA 282
            +GF  +RFDF +GY+ +  K Y+    P F+VGE WDS +Y   G  + NQD+HR  + 
Sbjct: 193 TVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGL-DYNQDSHRQRII 252

Query: 283 DWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSKGKPPGFIGIKPESAVTFIDNHDTG 342
            W+ A+G    AFDFTTKGILQ AV+G+ WRL D++GKPPG +G  P  AVTF+DNHDTG
Sbjct: 253 SWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTG 312

Query: 343 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWG--LKEEISKLSEIRARNGIGKK 402
           STQ  WPFPS  V++GYAYILTHPG P +FYDHF DWG  + ++I KL +IR R  I  +
Sbjct: 313 STQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSR 372

Query: 403 STVNIMASDGGLYVAGVDGKIIMKIGPNMDLGNLIPS--DYQLATSGMEYAVWEK 454
           STV ++ ++  LY A V  KI MK+G     G+  PS  D+ LATSG  YAVW K
Sbjct: 373 STVRVLKAESNLYAAIVGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVWHK 413

The following BLAST results are available for this feature:

BLAST of Spg017019 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142476.1	2.8e-201	78.02	alpha-amylase [Momordica charantia]	[more]
XP_038896126.1	4.1e-200	80.27	alpha-amylase-like [Benincasa hispida]	[more]
XP_022936258.1	1.1e-194	77.33	alpha-amylase, partial [Cucurbita moschata]	[more]
XP_023536126.1	5.7e-194	75.65	alpha-amylase, partial [Cucurbita pepo subsp. pepo]	[more]
XP_022976296.1	8.2e-193	76.84	alpha-amylase [Cucurbita maxima]	[more]

BLAST of Spg017019 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17859	3.8e-177	80.86	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]
P27934	1.1e-144	63.53	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]
P04063	1.5e-144	65.44	Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3	[more]
P27933	8.9e-142	63.53	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2...	[more]
Q8VZ56	3.4e-141	66.19	Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1	[more]

BLAST of Spg017019 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMA4	1.4e-201	78.02	Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1	[more]
A0A6J1FCS4	5.5e-195	77.33	Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1	[more]
A0A6J1IGI6	4.0e-193	76.84	Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1	[more]
A0A6A1W532	1.4e-177	82.34	Alpha-amylase OS=Morella rubra OX=262757 GN=CJ030_MR3G003095 PE=3 SV=1	[more]
A0A2I4EXA7	1.4e-174	69.30	Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1	[more]

BLAST of Spg017019 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25000.1	2.4e-142	66.19	alpha-amylase-like	[more]
AT1G69830.1	5.1e-92	48.01	alpha-amylase-like 3	[more]
AT1G76130.1	7.4e-91	47.04	alpha-amylase-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 134..145 score: 49.02 coord: 330..342 score: 51.43 coord: 226..237 score: 40.85
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 393..453 e-value: 9.3E-31 score: 118.2
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 394..452 e-value: 4.8E-17 score: 62.0
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 67..392 e-value: 1.2E-27 score: 107.9
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 115..239 e-value: 2.3E-12 score: 47.1
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 397..454 e-value: 6.6E-23 score: 82.6
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 89..396 e-value: 3.4E-125 score: 420.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 182..204
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 18..454
None	No IPR available	PANTHER	PTHR43447:SF37	ALPHA-AMYLASE 1	coord: 18..454
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 103..403 e-value: 4.2497E-144 score: 411.999
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 397..453
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 99..386

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg017019.1	Spg017019.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding