Spg016936 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016936
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCytochrome P450
Locationscaffold9: 45366291 .. 45370354 (-)
RNA-Seq ExpressionSpg016936
SyntenySpg016936
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAAATTCCGGCAAGATCCCGCCGTCTCCGTCGCATCGCGACAATGTTCCCCCGACCGTACTCTGCACCGCCCCATTCTTTTTTCCCTCCTCATTTAATATCTTACAGCATTAGAAACTCTGCCTTCTTTCTTTGCAAAAAAAACAACCAAGTTTTCTTTTATCCTGGCCCGCAACTCCTGAATTCAATGAATTTTTTAACACAAATTACAAGCTTTTTAACCGAATAGAAACAACAGGCCACCGCTTAAAAAAATAAACCAGACAAGATATAAAGTCACATAACAACCACCATAACGTCGTAATCCGCATTTATTTTATTTAAAAAAATTTCGTTGTTTAGTCATTTTGGTTAATCTTTCATTTGTAGTTAACAACAGGCAAGAAAATCAAAATTTGGAAAGAATAATTTATTATACGCAACCAATTAACCAACATCACACACTTTAATCCATTTTTAGTTTAGTTCAAATTACAAGTTTAGTAGTTCAGATTAATGCATATTTAGTCTCTAAACTTTAAATAAATTTGTAATTTAACTTATATTTTATAACAAAAAACGAGTGTCAGTAGAACAAGCTTGATTATTTAAGTAAGCAGCAAAGTTTCGAAGGATGCAGTGTTGGGACGAAGAGTGAGTGATAGTAGTGAAGCCATGGGAGTTTTAGGAGCTATATTTTTATACATTCCACTGTTTTTAGTGTTGTACACATTAACAGAGCAGTTTCTTCACAAAATTCGAAACCTCCCGCCGACCCCTTTCCCGTCGGTTCCAGTCATCGGCCATCTCCACCTTCTGAAGAAGCCCCTCTACCGGACTTTGGCGAAGATCTCGAACCGGTACGGCCCGGTTGTCTTTCTCCGGTTCGGATCACGCCAAGTTCTCGTGGTGTCGTCTCCTTCCGCGGCCGAAGAATGTCTGACCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTCCTCCTATCCAAGTGGTTCGGATACAACAACACGAACCTCGTCTGGTCATCGTATGGGGACCACTGGCGCAACCTCCGCCGAATCTCCACCGTTGAAGTCTTGTCCACTCATCGCCTTCAGATGCTCTCGACCGTCCGTCTGGAAGAGGTCAACTCTTTGATTCGGAGGCTCGCTAGAAGCGAAACCCAGGTGCATAACATGAAGAATGCGTTTTTCGATCTGACTTATAACATCATGTTGAGGATGATTGTCGGAAAGCGGTTTTACGGTGATGACGTGGACGATGCTGACGAGGCAAGATGTTATAGAGAGTTACAGGCTGAGTCAACTCGATTGAGTGGTAAGTCAAATATAGGAGATTTTATCCCGTTGATGTCGTGGCTGGGATTTGGATCGGCTGTGGAGAATGAGATAATGGAATGTCACAAAAGAAGAGACGCTTTCATGCAGAGTTTGATTGACCAGCAAAGGAAAAGAATGGTTCAAACTGAAACTGGAGATTCCTTTCGCGATGGAAGGAAGAAAACGATGATTGAAGTACTGTTAGAGCTACAAGAATCTCAACCTGAGCAGTACAATGACGAAACTATCAAAGCCTTGATGCTGGTAATCATTTTTCTTCTTTATTTAGTGTTCAATTTAACCTTGATTGCTTTGGTTGTGTTATTCAATTAGCCCACATATGCAAAATTCAAAATTTGAGAATATATTTAAACCAAGATTCAAAATTGACGTTGGACATCAAATTAAAAGTTCAAAAACTCGCTAGATAGTTTATGAGTATGTTTATGTTCAATCACACACACTATATATAACATGGTTTTTTAATCAAGTCTACCTCATATATTAAAAATAGTCTCTACATATTTTATTCAAGGATATGCATGAATCACAATTTGAATTTATGACATTTTCATTGTTTTTTTTTTTTTTTTTTTTTGTACACCATCTTTAAATAATTGATTTTTTATTATTATTTTAGTTCAAATAATTTAAAGTAGGACATATTAGAGACTTCTTAAAATATAGGTAAATGTTTATAATGCATGTGATTTTCTTTATTTTCTTTATCCTATATAAAAAATTTGAATATCTCACTTTTTAATCAACAATACATGTTTTATGTGAGTTGAGTTGTACTTGTGTTCGTTATGTGATTTAGTTTAAGATTCAATAGTTAGTCTTTATTTAATTATTTTAGTTTCCGTTCATCACATAAGTTCTGGTGTCACCCAACTTAGATTAAAGCATTGATTCGGGGAAAAGACAAAAGCAATTAAAATAATACGAAATTATGAAGATGTGAATGTGATTGGTGATAAGTTTCAATGGTTAATTTGTCTCGTAGAGGCCACTCCGGGTTTAAATTATATAACTAATATTTGGGTGACTTCAAAGTTTTGTCGTGAATGGAGATGAGTGACTCCGAAAAAGAAAAAAGAAAAATATTTTTATTTTCAGTACAGAAGAAAAATGTGTTTTTTTTAAGAAGAAAATGTTATTTTATACGACTATATCACCGATTTTTTTACATGAGTGGTCGAACTTTACATGGTATCCAGTTGTAAAGCATTTATTATTATTATTATTATTTTGTCTAGATAAGTATATTGATTTGATATGCATTTGAACTTTTTTTTTTAGAAAAAATGCTATGTATTTGAATAACTATTCATTCTTTTCAAAAAGAAAATGAAATTAAATATCCATCCATCTGCGGTGGATAAGACACCCATTAAAATTATAAACTAACTAATCTCTCCTATTAAAAAGCTTAAAATCTTTCAGCTTTGGCCACGTAGTCAACTTATTCCAATAATTTATTTCTATCTTTAAAAAGCTTAAACTATTATTATTATTAAAAAAAAAACTATTATTATTTTAAAAAGTTAGAATTACATATTTAATCTCTAAACTTTTAGGTTTGTGACTATTTAGTACTTAAACTTTTAAAAGTATTTAATAAGTTTCTCAACTTTCATTTTGTTTATTTGGTTTAAAAACTTTAAAAAGTATCTAATAAATTATTAAACTTTCAACTTTGTGTCGAATAAGTCTTTGAATTTCCAATTTTATGTCTAAAAGTCATTGAGCTATTTGATGTTTTAATTTTAAAATTCATGTATCTACTAGACACAAAATTGAAAATTTTTAAGGTTCTATGGAACATAAATACAAAATTAAAAATTCAGAGATTTATTAGATATTTTTAGAGTTTGTAACCCTCATAAAATTGAAAGTCTAGAAACTAGAAACTCTTTAAATGTAGGTATCAAACAAACACGGACTTATAAATTCAAATATTACACTTTAATTAAACTGTTTTTAAAGCTATGTACTGTATATGTATTTATATTGTTTATTTATTTATTCGAAATAATGTATTTATAGTGTTTATATTAATTCCGTAGCTGTTTGAATATTTAATATTTAGTATGTACGTGGACAGGTTCTGTTGGCTGCAGGTACGGAAACATCAGTGAACACGATGGAATGGGCCCTATCTCTTATGTTGAACCAACCAGAAGTTTTAAAGAAAGCGCAGATCGAAATTGACTGTCAAGTTGGCCAAGACCGTCTGATTGACGAATCTGACGTGGCGAACCTTCCCTACCTACGTGGCATCATCAACGAAACCTTACGAATGTACCCTCCAGGTCCACTGATGGTACCCCACGAGTCATCAAAAGACTGTAGCGTTGGAGGATACCATATCCCACGTGGCACTATGCTGTACGTGAATTTATGGGCGATTCATAACGACCCCAAGAATTGGGGTGAGCCGAGAAAGTTTGATCCCGAGAGGTTTGAGAGGGTGGAAAGTGAGAAGTATGGGTTCAATCTGATGCCGTTCGGGGCGGGTAGAAGGGGCTGTCCAGGAGAGGGATTGGGGCTTCGCATGATTGGGCTTGTAATGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGCGAGGAGTTGGTGGATATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACGCTTGCTCGCTAAATGTACGCCTCGACCCATTGCCGAACATCTTGATCTTTCTCCAACCAGATCCAACCCGGCCCATTATAAAAATTGA

mRNA sequence

ATGACAAAATTCCGGCAAGATCCCGCCGTCTCCGTCGCATCGCGACAATTGTTGGGACGAAGAGTGAGTGATAGTAGTGAAGCCATGGGAGTTTTAGGAGCTATATTTTTATACATTCCACTGTTTTTAGTGTTGTACACATTAACAGAGCAGTTTCTTCACAAAATTCGAAACCTCCCGCCGACCCCTTTCCCGTCGGTTCCAGTCATCGGCCATCTCCACCTTCTGAAGAAGCCCCTCTACCGGACTTTGGCGAAGATCTCGAACCGGTACGGCCCGGTTGTCTTTCTCCGGTTCGGATCACGCCAAGTTCTCGTGGTGTCGTCTCCTTCCGCGGCCGAAGAATGTCTGACCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTCCTCCTATCCAAGTGGTTCGGATACAACAACACGAACCTCGTCTGGTCATCGTATGGGGACCACTGGCGCAACCTCCGCCGAATCTCCACCGTTGAAGTCTTGTCCACTCATCGCCTTCAGATGCTCTCGACCGTCCGTCTGGAAGAGGTCAACTCTTTGATTCGGAGGCTCGCTAGAAGCGAAACCCAGGTGCATAACATGAAGAATGCGTTTTTCGATCTGACTTATAACATCATGTTGAGGATGATTGTCGGAAAGCGGTTTTACGGTGATGACGTGGACGATGCTGACGAGGCAAGATGTTATAGAGAGTTACAGGCTGAGTCAACTCGATTGAGTGGTAAGTCAAATATAGGAGATTTTATCCCGTTGATGTCGTGGCTGGGATTTGGATCGGCTGTGGAGAATGAGATAATGGAATGTCACAAAAGAAGAGACGCTTTCATGCAGAGTTTGATTGACCAGCAAAGGAAAAGAATGGTTCAAACTGAAACTGGAGATTCCTTTCGCGATGGAAGGAAGAAAACGATGATTGAAGTACTGTTAGAGCTACAAGAATCTCAACCTGAGCAGTACAATGACGAAACTATCAAAGCCTTGATGCTGGTTCTGTTGGCTGCAGGTACGGAAACATCAGTGAACACGATGGAATGGGCCCTATCTCTTATGTTGAACCAACCAGAAGTTTTAAAGAAAGCGCAGATCGAAATTGACTGTCAAGTTGGCCAAGACCGTCTGATTGACGAATCTGACGTGGCGAACCTTCCCTACCTACGTGGCATCATCAACGAAACCTTACGAATGTACCCTCCAGGTCCACTGATGGTACCCCACGAGTCATCAAAAGACTGTAGCGTTGGAGGATACCATATCCCACGTGGCACTATGCTGTACGTGAATTTATGGGCGATTCATAACGACCCCAAGAATTGGGGTGAGCCGAGAAAGTTTGATCCCGAGAGGTTTGAGAGGGTGGAAAGTGAGAAGTATGGGTTCAATCTGATGCCGTTCGGGGCGGGTAGAAGGGGCTGTCCAGGAGAGGGATTGGGGCTTCGCATGATTGGGCTTGTAATGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGCGAGGAGTTGGTGGATATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACGCTTGCTCGCTAAATGTACGCCTCGACCCATTGCCGAACATCTTGATCTTTCTCCAACCAGATCCAACCCGGCCCATTATAAAAATTGA

Coding sequence (CDS)

ATGACAAAATTCCGGCAAGATCCCGCCGTCTCCGTCGCATCGCGACAATTGTTGGGACGAAGAGTGAGTGATAGTAGTGAAGCCATGGGAGTTTTAGGAGCTATATTTTTATACATTCCACTGTTTTTAGTGTTGTACACATTAACAGAGCAGTTTCTTCACAAAATTCGAAACCTCCCGCCGACCCCTTTCCCGTCGGTTCCAGTCATCGGCCATCTCCACCTTCTGAAGAAGCCCCTCTACCGGACTTTGGCGAAGATCTCGAACCGGTACGGCCCGGTTGTCTTTCTCCGGTTCGGATCACGCCAAGTTCTCGTGGTGTCGTCTCCTTCCGCGGCCGAAGAATGTCTGACCAAGAACGACATCGTGTTTGCGAACCGGCCTCGCCTCCTCCTATCCAAGTGGTTCGGATACAACAACACGAACCTCGTCTGGTCATCGTATGGGGACCACTGGCGCAACCTCCGCCGAATCTCCACCGTTGAAGTCTTGTCCACTCATCGCCTTCAGATGCTCTCGACCGTCCGTCTGGAAGAGGTCAACTCTTTGATTCGGAGGCTCGCTAGAAGCGAAACCCAGGTGCATAACATGAAGAATGCGTTTTTCGATCTGACTTATAACATCATGTTGAGGATGATTGTCGGAAAGCGGTTTTACGGTGATGACGTGGACGATGCTGACGAGGCAAGATGTTATAGAGAGTTACAGGCTGAGTCAACTCGATTGAGTGGTAAGTCAAATATAGGAGATTTTATCCCGTTGATGTCGTGGCTGGGATTTGGATCGGCTGTGGAGAATGAGATAATGGAATGTCACAAAAGAAGAGACGCTTTCATGCAGAGTTTGATTGACCAGCAAAGGAAAAGAATGGTTCAAACTGAAACTGGAGATTCCTTTCGCGATGGAAGGAAGAAAACGATGATTGAAGTACTGTTAGAGCTACAAGAATCTCAACCTGAGCAGTACAATGACGAAACTATCAAAGCCTTGATGCTGGTTCTGTTGGCTGCAGGTACGGAAACATCAGTGAACACGATGGAATGGGCCCTATCTCTTATGTTGAACCAACCAGAAGTTTTAAAGAAAGCGCAGATCGAAATTGACTGTCAAGTTGGCCAAGACCGTCTGATTGACGAATCTGACGTGGCGAACCTTCCCTACCTACGTGGCATCATCAACGAAACCTTACGAATGTACCCTCCAGGTCCACTGATGGTACCCCACGAGTCATCAAAAGACTGTAGCGTTGGAGGATACCATATCCCACGTGGCACTATGCTGTACGTGAATTTATGGGCGATTCATAACGACCCCAAGAATTGGGGTGAGCCGAGAAAGTTTGATCCCGAGAGGTTTGAGAGGGTGGAAAGTGAGAAGTATGGGTTCAATCTGATGCCGTTCGGGGCGGGTAGAAGGGGCTGTCCAGGAGAGGGATTGGGGCTTCGCATGATTGGGCTTGTAATGGGCTCACTGATCCAGTGCTTTGAATGGGAGCGTCCTGGCGAGGAGTTGGTGGATATGACAGAAGGCATTGCAGTCTCTATGCCCAAGGCCCAACGCTTGCTCGCTAAATGTACGCCTCGACCCATTGCCGAACATCTTGATCTTTCTCCAACCAGATCCAACCCGGCCCATTATAAAAATTGA

Protein sequence

MTKFRQDPAVSVASRQLLGRRVSDSSEAMGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAHYKN
Homology
BLAST of Spg016936 vs. NCBI nr
Match: XP_022940352.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 868.2 bits (2242), Expect = 3.7e-248
Identity = 414/514 (80.54%), Postives = 471/514 (91.63%), Query Frame = 0

Query: 32  LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
           +GAI +YIPLF VLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 92  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLA+ E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLR 180

Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMEC 240

Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
           H++RDAFMQ+LI+Q + R ++TE  +SFRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
           F +VESEK GF LMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGEELVDMTEG+
Sbjct: 421 FVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGV 480

Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
           AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of Spg016936 vs. NCBI nr
Match: XP_022982225.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 866.3 bits (2237), Expect = 1.4e-247
Identity = 413/514 (80.35%), Postives = 471/514 (91.63%), Query Frame = 0

Query: 32  LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
           +GAI LYIPLFLVLY LTEQF+HKIRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 92  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLAR E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180

Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMEC 240

Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
           H++RDAFMQ+LI+Q + R ++TE  + FRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
           F +V+SEK GF L+PFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480

Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
           AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of Spg016936 vs. NCBI nr
Match: XP_023523281.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.0 bits (2218), Expect = 2.2e-245
Identity = 410/514 (79.77%), Postives = 469/514 (91.25%), Query Frame = 0

Query: 32  LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
           +GAI +YIPLFLVLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIIVYIPLFLVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 92  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
           WRNLRRISTVE+LSTHRLQML+ VR +EV SLI RLAR E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRSDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180

Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+ DFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMEDFIPLMSWLGLGWAAEKEIMEC 240

Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
           H++RDAFMQ+LI+Q + R ++TE  +SFRDGRKKT+IEVL+ELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLVELQESDPDQYNDETIRALM 300

Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
           INETLRMYPP PLMVPHESSKDCSVGGY IPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYQIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
           F +V+SEK+GF LMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKHGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480

Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
           AVSMPKA+ L AKC PRPI   L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVARLHLSQTKSNLAY 514

BLAST of Spg016936 vs. NCBI nr
Match: XP_022146589.1 (cytochrome P450 81D1-like [Momordica charantia])

HSP 1 Score: 845.9 bits (2184), Expect = 2.0e-241
Identity = 416/528 (78.79%), Postives = 474/528 (89.77%), Query Frame = 0

Query: 26  SEAMGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLA 85
           S+ +  +GAIFLY PLFLVLY LTE FLHKIRNLPPTPFPS+P+IGHL+LL KP+YRTLA
Sbjct: 3   SKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLA 62

Query: 86  KISNRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVW 145
            ISNR+GPVVFLRFGSR VLVVSSPS AEECLTKND+VFANRPRLL+SK FGYNNTNLVW
Sbjct: 63  DISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVW 122

Query: 146 SSYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQ--VHNMKNAFFD 205
           SSYGDHWR+LRRISTVEVLSTHRLQ+LSTVRLEEV SLIR+LA+SE Q  +  M+++FFD
Sbjct: 123 SSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFD 182

Query: 206 LTYNIMLRMIVGKRFYGDDVDDADE-ARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGS 265
           LT+N+MLRMI GKRFYGDDVDDADE A+ +RELQAESTRL+G SNIGDF+P+M+WLGFG 
Sbjct: 183 LTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 242

Query: 266 AVENEIMECHKRRDAFMQSLIDQQRKRM---VQTETGDSFRDGRKKTMIEVLLELQESQP 325
           +VE ++M+CH +RDAFMQ+LI++QRKR     ++E  DSFRDGR+KTMIEVLLELQES+P
Sbjct: 243 SVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEP 302

Query: 326 EQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDE 385
           + Y DE+IKALMLVLL AGTETSVNTMEWALSL+LN PEVLKKAQ+EID QVGQDRLIDE
Sbjct: 303 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDBQVGQDRLIDE 362

Query: 386 SDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPK 445
           SD+A+LPYLRGII ET RMYPP PLMVPH+SS+DCSVGG+HIPRGTMLYVNLWAIHNDPK
Sbjct: 363 SDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPK 422

Query: 446 NWGEPRKFDPERFERVESEK--YGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEW 505
            W  P KFDP+RF+R+ES+K  YGFNLMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEW
Sbjct: 423 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 482

Query: 506 ERPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
           ERPGE+LVDMTEGIAVSMPKAQ L AKC PRPI  +L  S T++N AH
Sbjct: 483 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529

BLAST of Spg016936 vs. NCBI nr
Match: XP_008453068.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 830.5 bits (2144), Expect = 8.6e-237
Identity = 398/516 (77.13%), Postives = 450/516 (87.21%), Query Frame = 0

Query: 29  MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
           MGVLG+IF+Y PLFLVLY L E  LHKIRNLPPTPFP +P+IGHLHLLKKP+YRTLAKIS
Sbjct: 1   MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60

Query: 89  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 148
           N YGPVV+ RFGSR+VLVVSS  AAEECLTKND+VFANRPRL++SKWFGYNNTNL+WSSY
Sbjct: 61  NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120

Query: 149 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 208
           GDHWRNLRRIST+E+LSTHRLQM S+VRLEEV SLIRRLA  E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180

Query: 209 MLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 268
           MLRM+VGKR+YG+DVDD DEA+ +R+LQ +  +L GKS + DFIPL+SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240

Query: 269 MECHKRRDAFMQSLIDQQRKR-MVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 328
           +E H +RD FMQ+LIDQ +KR   Q  +   ++DGR+KTMIEVLLELQ+ +P QY DETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300

Query: 329 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPY 388
           +ALML+LLAAGTETSV TMEWALSLMLN PE LKK Q EID QVG DRLIDESD+ANLP 
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360

Query: 389 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 448
           LRGIINET RMYPP PLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHNDPK W  PRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420

Query: 449 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDM 508
           +P RFE +ESEK+GFNLMPFG+GRRGCPGEGLGLR+IGLV+GSL+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480

Query: 509 TEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNP 544
           TEGIA++MPKA  L AKCTPRPI   L LS   SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516

BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.4e-133
Identity = 249/500 (49.80%), Postives = 333/500 (66.60%), Query Frame = 0

Query: 37  LYIPLFLVLYTLTEQFL---HKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGP 96
           LY  L +VL +++ +      + RNLPP+P  ++PVIGHLHL+ K L+R+L  +S +YG 
Sbjct: 7   LYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGS 66

Query: 97  VVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWR 156
           V  L+ G+R VLVVSSP+AAEEC TKNDIVFANRP  +L K+ GYN T +V S YG+HWR
Sbjct: 67  VFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWR 126

Query: 157 NLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIML 216
           NLRR++ VE+ S   L    ++R +EV  L+  L +S  Q      MK+   +L++N+ +
Sbjct: 127 NLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTM 186

Query: 217 RMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIME 276
           RM+ GKR++G DV D+DEA+ +R L  E    +G SN GDF+P + W+ F +  E ++ +
Sbjct: 187 RMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKN-YEKKVSK 246

Query: 277 CHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKAL 336
             +  DAF+Q LI + R             +    TMI+ LL LQESQPE Y D+ IK +
Sbjct: 247 ISQEMDAFLQRLIHESR------------INKNNVTMIDHLLSLQESQPEYYTDQIIKGI 306

Query: 337 MLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRG 396
           ++VLL AGT+TS  T+EWA+SL+LN PE L+KA+ EI+ QVG +RLI+E D+  L YL  
Sbjct: 307 IMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHN 366

Query: 397 IINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPE 456
           II+ET R+ P  P++VPHESS DC V GY +P+GT+L VN WAIH DP+ W EP  F PE
Sbjct: 367 IISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPE 426

Query: 457 RFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEG 516
           R   VE E     LMPFG GRR CPG GL  R++GL +G+LIQCFEW+R GE  +DM EG
Sbjct: 427 RHGGVELEP--SKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEG 486

Query: 517 IAVSMPKAQRLLAKCTPRPI 531
             ++MPKAQ L A C PR I
Sbjct: 487 SGLTMPKAQPLEALCKPRNI 490

BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 240/508 (47.24%), Postives = 335/508 (65.94%), Query Frame = 0

Query: 38  YIPLFLVLYTLTEQFLHKI-----RNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG 97
           Y  L  + + +T + L KI     +NLPP P P++P+IG+LH LK PL+RT   +S  YG
Sbjct: 4   YYSLLSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYG 63

Query: 98  PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHW 157
            +  L FGSR V+VVSSPS A EC TKNDI+ ANRPR L  K+  YN T L  +SYGDHW
Sbjct: 64  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 123

Query: 158 RNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLAR---SE----TQVHNMKNAFFDLT 217
           RNLRRI+T++VLS +RL     VR +E N LI++L +   SE    T+V  ++    ++T
Sbjct: 124 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKV-ELRPRLTEMT 183

Query: 218 YNIMLRMIVGKRFYGD--DVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSA 277
           +N M+RMI GKR+YGD  DV D +EA+ +RE+ +E   L G +N GDF+PL+  +   + 
Sbjct: 184 FNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN- 243

Query: 278 VENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYN 337
           +E       KR +AF++ LI++ R+  + ++ G         TMI+ LL+L ESQPE Y+
Sbjct: 244 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGG---------TMIDHLLKLSESQPEYYS 303

Query: 338 DETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVA 397
           D  IK L+  +L AGT+TS  T+EW +S +LN PEVLKKA+ E+D Q+G+++L+DE D++
Sbjct: 304 DHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLS 363

Query: 398 NLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGE 457
            LPYL+ II+ETLR++PP PL++PH SS+DC++G +++P+ T++  N+W IH DPK+W +
Sbjct: 364 KLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWND 423

Query: 458 PRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEE 517
              F PERFE+ E       +M FG GRR CPG  L  R +G  +G LIQCFEWER  EE
Sbjct: 424 ALSFKPERFEKEEEVN---KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEE 483

Query: 518 LVDMTEGIAVSMPKAQRLLAKCTPRPIA 532
            +DM EG  ++MP    L A C   PIA
Sbjct: 484 KLDMMEGKGITMPMKIPLRAMCKALPIA 496

BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.3e-126
Identity = 238/500 (47.60%), Postives = 322/500 (64.40%), Query Frame = 0

Query: 37  LYIPLFLVLYTLTEQF--LHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGPV 96
           L I LF ++ TL   F    K +NLPP P   +P+IG+LH LK+PL+ T   +S +YG +
Sbjct: 8   LIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKYGQI 67

Query: 97  VFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRN 156
             L FGSR V+VVSS + A+EC TKNDIV ANRP  L  K+ GYNNT +  S YGDHWRN
Sbjct: 68  FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 127

Query: 157 LRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSE----TQVHNMKNAFFDLTYNIML 216
           LRRI ++E+LS+HRL     +R +E+  LI++LA+      T+V  ++  F ++T+N ++
Sbjct: 128 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEV-ELRPMFSEMTFNTIM 187

Query: 217 RMIVGKRFYGDDVD--DADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 276
           RM+ GKR+YG+D D  D +EAR +R +  E   L G +N+GDF+  + W  F   +E  +
Sbjct: 188 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDF-DGLEKRL 247

Query: 277 MECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIK 336
            +  KR DAF+Q LID+ R           F      TMI+ LL  Q+SQPE Y D+ IK
Sbjct: 248 KKISKRTDAFLQGLIDEHR-----------FGKRNSNTMIDHLLTQQQSQPEYYTDQIIK 307

Query: 337 ALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYL 396
            LM+V+L AGT+TS  T+EWA+S +LN PE++KKA+ E+D  +G DR +DE D++ LPYL
Sbjct: 308 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 367

Query: 397 RGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFD 456
           + I+ ETLR++   PL+VPH SS+D S+GGY+IP+ T+L VN W IH DP  W +P  F 
Sbjct: 368 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 427

Query: 457 PERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMT 516
           PERFE+   E     L+ FG GRR CPGE L  R  GL +G LIQCFEW+R GEE +DM 
Sbjct: 428 PERFEK---EGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMV 487

Query: 517 EGIAVSMPKAQRLLAKCTPR 529
           E   ++  K   L A C  R
Sbjct: 488 EAKGITAGKKTSLNAMCKVR 490

BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.7e-126
Identity = 243/505 (48.12%), Postives = 329/505 (65.15%), Query Frame = 0

Query: 35  IFLYIPLFLVLYTLTEQFLH-KIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS----- 94
           + LY    L+   ++ +FL  K +NLPP+P   +P+IGHL LLK P++RTL   S     
Sbjct: 8   VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67

Query: 95  NRYGPVVFLRFGSRQVLVVSSPS-AAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSS 154
           N  G V+ LR GSR V VVSS   AAEEC  KND+V ANRP++++ K  GYNNTN++ + 
Sbjct: 68  NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127

Query: 155 YGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLAR---SETQVHNMKNAFFDL 214
           YGDHWRNLRR+ T+E+ STHRL     VR +EV  LI RL+R   ++  V  +K    DL
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187

Query: 215 TYNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAV 274
           T+N ++RM+ GKR+YG++  D +EA+  R+L A+    +   N  D++P+   L   S+ 
Sbjct: 188 TFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI---LRLFSSY 247

Query: 275 ENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYND 334
           EN + +  +  D F+Q LID +R    Q ETG         TMI+ LL LQ+S  E Y D
Sbjct: 248 ENRVKKLGEETDKFLQGLIDDKRG---QQETG--------TTMIDHLLVLQKSDIEYYTD 307

Query: 335 ETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVAN 394
           + IK ++L+++ AGT TS  T+EWALS +LN P+V+ KA+ EID +VG DRLI+E+D++ 
Sbjct: 308 QIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSE 367

Query: 395 LPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEP 454
           LPYL+ I+ ETLR++P  PL+VPH +S+DC +G Y +PRGT L VN WAIH DP  W +P
Sbjct: 368 LPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDP 427

Query: 455 RKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEEL 514
             F PERFE+ E  +    L+ FG GRR CPG GL  R++GL +GSLIQCFEWER G   
Sbjct: 428 DSFKPERFEKEEEAQ---KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVE 487

Query: 515 VDMTEGIAVSMPKAQRLLAKCTPRP 530
           VDM EG+  ++PKA  L A C  RP
Sbjct: 488 VDMKEGVGNTVPKAIPLKAICKARP 495

BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 448.4 bits (1152), Expect = 1.2e-124
Identity = 235/516 (45.54%), Postives = 334/516 (64.73%), Query Frame = 0

Query: 36  FLYIP-LFLV-----LYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISN 95
           F+ +P LFLV     LY+ T++F     NLPP P PS P +GHLHL+K P++R L + SN
Sbjct: 4   FILLPLLFLVISYKFLYSKTQRF-----NLPPGP-PSRPFVGHLHLMKPPIHRLLQRYSN 63

Query: 96  RYGPVVFLRFGSRQVLVVSSPSAAEECLT-KNDIVFANRPRLLLSKWFGYNNTNLVWSSY 155
           +YGP+  LRFGSR+V+V++SPS A+E  T +NDIV ++RP  L +K+  YN+T +  + Y
Sbjct: 64  QYGPIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPY 123

Query: 156 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAF------- 215
           GDHWRNLRRI + E+LS+HRL     +R +E+  ++ RL+R  TQ  N  N F       
Sbjct: 124 GDHWRNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSR-YTQTSNESNDFTHIELEP 183

Query: 216 --FDLTYNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLG 275
              DLT+N ++RM+ GKR+YGDDV++ +EA  +++L  +    SG ++  D++P++    
Sbjct: 184 LLSDLTFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKL-- 243

Query: 276 FGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQP 335
           FG+  E E+    K  D  +Q L+D+ R            RD    TM+  L+ LQ+ QP
Sbjct: 244 FGNKFEKEVKAIGKSMDDILQRLLDECR------------RDKEGNTMVNHLISLQQQQP 303

Query: 336 EQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDE 395
           E Y D  IK LM+ ++ AGTETS  T+EWA++ +L  PEVL+KA+ EID ++G+DRLIDE
Sbjct: 304 EYYTDVIIKGLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDE 363

Query: 396 SDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPK 455
           SD+A LPYL+ +++ET R++P  P ++P   + D  +GGY +PR T++ VN WAIH DP+
Sbjct: 364 SDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPE 423

Query: 456 NWGEPRKFDPERF-ERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWE 515
            W EP KF+P+R+ +   S+ Y + LMPFG GRR CPG GLG R++ L +GSLIQCFEWE
Sbjct: 424 IWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWE 483

Query: 516 RPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIAEHL 535
               E +DM+E   + M K   L A C PRPI   L
Sbjct: 484 NVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSKL 498

BLAST of Spg016936 vs. ExPASy TrEMBL
Match: A0A6J1FJC9 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445994 PE=3 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.8e-248
Identity = 414/514 (80.54%), Postives = 471/514 (91.63%), Query Frame = 0

Query: 32  LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
           +GAI +YIPLF VLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 92  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLA+ E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLR 180

Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMEC 240

Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
           H++RDAFMQ+LI+Q + R ++TE  +SFRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
           F +VESEK GF LMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGEELVDMTEG+
Sbjct: 421 FVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGV 480

Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
           AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of Spg016936 vs. ExPASy TrEMBL
Match: A0A6J1J228 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 6.8e-248
Identity = 413/514 (80.35%), Postives = 471/514 (91.63%), Query Frame = 0

Query: 32  LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
           +GAI LYIPLFLVLY LTEQF+HKIRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1   MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60

Query: 92  GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
           GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61  GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120

Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
           WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLAR E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180

Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
           MIVGKRFYG+DV+DADEA  +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMEC 240

Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
           H++RDAFMQ+LI+Q + R ++TE  + FRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300

Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
           LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360

Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
           INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420

Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
           F +V+SEK GF L+PFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480

Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
           AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514

BLAST of Spg016936 vs. ExPASy TrEMBL
Match: A0A6J1CYI5 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 9.5e-242
Identity = 416/528 (78.79%), Postives = 474/528 (89.77%), Query Frame = 0

Query: 26  SEAMGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLA 85
           S+ +  +GAIFLY PLFLVLY LTE FLHKIRNLPPTPFPS+P+IGHL+LL KP+YRTLA
Sbjct: 3   SKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLA 62

Query: 86  KISNRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVW 145
            ISNR+GPVVFLRFGSR VLVVSSPS AEECLTKND+VFANRPRLL+SK FGYNNTNLVW
Sbjct: 63  DISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVW 122

Query: 146 SSYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQ--VHNMKNAFFD 205
           SSYGDHWR+LRRISTVEVLSTHRLQ+LSTVRLEEV SLIR+LA+SE Q  +  M+++FFD
Sbjct: 123 SSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFD 182

Query: 206 LTYNIMLRMIVGKRFYGDDVDDADE-ARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGS 265
           LT+N+MLRMI GKRFYGDDVDDADE A+ +RELQAESTRL+G SNIGDF+P+M+WLGFG 
Sbjct: 183 LTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 242

Query: 266 AVENEIMECHKRRDAFMQSLIDQQRKRM---VQTETGDSFRDGRKKTMIEVLLELQESQP 325
           +VE ++M+CH +RDAFMQ+LI++QRKR     ++E  DSFRDGR+KTMIEVLLELQES+P
Sbjct: 243 SVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEP 302

Query: 326 EQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDE 385
           + Y DE+IKALMLVLL AGTETSVNTMEWALSL+LN PEVLKKAQ+EID QVGQDRLIDE
Sbjct: 303 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDE 362

Query: 386 SDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPK 445
           SD+A+LPYLRGII ET RMYPP PLMVPH+SS+DCSVGG+HIPRGTMLYVNLWAIHNDPK
Sbjct: 363 SDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPK 422

Query: 446 NWGEPRKFDPERFERVESEK--YGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEW 505
            W  P KFDP+RF+R+ES+K  YGFNLMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEW
Sbjct: 423 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 482

Query: 506 ERPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
           ERPGE+LVDMTEGIAVSMPKAQ L AKC PRPI  +L  S T++N AH
Sbjct: 483 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529

BLAST of Spg016936 vs. ExPASy TrEMBL
Match: A0A1S3BUR6 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 4.1e-237
Identity = 398/516 (77.13%), Postives = 450/516 (87.21%), Query Frame = 0

Query: 29  MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
           MGVLG+IF+Y PLFLVLY L E  LHKIRNLPPTPFP +P+IGHLHLLKKP+YRTLAKIS
Sbjct: 1   MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60

Query: 89  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 148
           N YGPVV+ RFGSR+VLVVSS  AAEECLTKND+VFANRPRL++SKWFGYNNTNL+WSSY
Sbjct: 61  NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120

Query: 149 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 208
           GDHWRNLRRIST+E+LSTHRLQM S+VRLEEV SLIRRLA  E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180

Query: 209 MLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 268
           MLRM+VGKR+YG+DVDD DEA+ +R+LQ +  +L GKS + DFIPL+SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240

Query: 269 MECHKRRDAFMQSLIDQQRKR-MVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 328
           +E H +RD FMQ+LIDQ +KR   Q  +   ++DGR+KTMIEVLLELQ+ +P QY DETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300

Query: 329 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPY 388
           +ALML+LLAAGTETSV TMEWALSLMLN PE LKK Q EID QVG DRLIDESD+ANLP 
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360

Query: 389 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 448
           LRGIINET RMYPP PLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHNDPK W  PRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420

Query: 449 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDM 508
           +P RFE +ESEK+GFNLMPFG+GRRGCPGEGLGLR+IGLV+GSL+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480

Query: 509 TEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNP 544
           TEGIA++MPKA  L AKCTPRPI   L LS   SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516

BLAST of Spg016936 vs. ExPASy TrEMBL
Match: A0A0A0L2I7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 3.9e-235
Identity = 391/516 (75.78%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 29  MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
           MGVL +IF+YIPLF VLYTL E  +HKIRNLPPTPFP +PVIGHLHLLK P+YRTLA+IS
Sbjct: 1   MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60

Query: 89  NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 148
           N YGPVV+ RFG R+VLVVSSP AAEECLTKNDIVFANRPRL++SKWFGYNNTNLVWSSY
Sbjct: 61  NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPRLIISKWFGYNNTNLVWSSY 120

Query: 149 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 208
           GDHWRNLRRIST+E+LSTHR+QMLS  RLEEV SLIRRLA +E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNENQIHNMKNEFFDLTYNV 180

Query: 209 MLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 268
           MLRM+VGKRFYG+DVDD DEA+ +R+LQ +  +L GKS + DFIP +SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240

Query: 269 MECHKRRDAFMQSLIDQQRKRMV-QTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 328
           +ECH +RD FMQ+LIDQ +KR+V Q ++ +  +DGR+KTMIEVLLELQ+  P+QY DETI
Sbjct: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300

Query: 329 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPY 388
           +ALMLVLLAAGTETSV  MEWALSLMLN P+ LKK Q EID QVG DRLIDESD+ANLP 
Sbjct: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360

Query: 389 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 448
           LRGIINETLRMYPP PL+VPHESSKDC++GGYHIPRGT+L+VNLWAIHNDPK W  PRKF
Sbjct: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420

Query: 449 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDM 508
           +P RFE +E+EK+GFNL+PFG+GRRGCPGEGL LR+IGLV+G+L+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480

Query: 509 TEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNP 544
           TEG+A++MPKA  L AKCTPRPI   L LS   SNP
Sbjct: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 516

BLAST of Spg016936 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 471.9 bits (1213), Expect = 7.2e-133
Identity = 247/506 (48.81%), Postives = 331/506 (65.42%), Query Frame = 0

Query: 29  MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
           M  L  IF +  + L L  L  +   K+ NLPP+P  ++PVIGHL LLK PL+R    +S
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRKL-NLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 89  NRYG--PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWS 148
              G  P++ LR G+R + VVSS S AEEC TKND++ ANR   + +K   Y N+ +V +
Sbjct: 61  QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120

Query: 149 SYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSE----TQVHNMKNAFF 208
           SY +HWRNLRRI  +E+ S HRL   S++R +E+  LI RL R+     T+V  MK+ F 
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKV-EMKSMFS 180

Query: 209 DLTYNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGS 268
           DLT+N ++RM+ GK +YGD  +D  EA+  R L AE+   SG  N  D+IP+++W+ +  
Sbjct: 181 DLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS- 240

Query: 269 AVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQY 328
             E  I +   R D F+Q L+D++R+           ++ ++ TM++ LL LQE+QPE Y
Sbjct: 241 --ETRIKKLAGRLDEFLQGLVDEKREG----------KEKKENTMVDHLLCLQETQPEYY 300

Query: 329 NDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDV 388
            D  IK  ML L+A GT+T+  T+EWALS +LN PEVL KA+ EID  +G DRL++ESD+
Sbjct: 301 MDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDI 360

Query: 389 ANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWG 448
            NLPYL+ I++ETLR+YP  P+++PH +SKDC VGGY +PRGTML  N WAIH DP  W 
Sbjct: 361 PNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWD 420

Query: 449 EPRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGE 508
           +P  F PERFE+    K    LMPFG GRR CPG GL  R++ L +GSLIQCFEWER GE
Sbjct: 421 DPTSFKPERFEKEGEAK---KLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE 480

Query: 509 ELVDMTEGIAVSMPKAQRLLAKCTPR 529
           E VDMTEG  ++MPKA+ L A C  R
Sbjct: 481 EEVDMTEGPGLTMPKARPLEAMCRAR 488

BLAST of Spg016936 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 468.4 bits (1204), Expect = 8.0e-132
Identity = 248/501 (49.50%), Postives = 329/501 (65.67%), Query Frame = 0

Query: 36  FLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG--P 95
           FL+I L L   T     + +  NLPP+P  ++PVIGHL LLK PL+R    +S   G  P
Sbjct: 10  FLFISLSL---TFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAP 69

Query: 96  VVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWR 155
           ++ LR G+R V VVSS S AEEC TKND+V ANR   L SK   Y  T +V +SYGDHWR
Sbjct: 70  IISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWR 129

Query: 156 NLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARS---ETQVHNMKNAFFDLTYNIML 215
           NLRRI  VE+ S HRL   S++R +E++ LI  L+R+   E     MK+ F +LT+N ++
Sbjct: 130 NLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNII 189

Query: 216 RMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIME 275
           RM+ GK +YGD  +D  EA+  REL AE     G  N  D++P+++W+   +  E  I +
Sbjct: 190 RMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIKK 249

Query: 276 CHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKAL 335
              R D F+Q L+D++R+           ++ R+ TM++ LL LQE+QPE Y D  IK +
Sbjct: 250 IASRLDEFLQGLVDERREG----------KEKRQNTMVDHLLCLQETQPEYYTDNIIKGI 309

Query: 336 MLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRG 395
           ML L+ AGT+TS  T+EW LS +LN P++L KA+ EID +VG +RL++ESD+++LPYL+ 
Sbjct: 310 MLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQN 369

Query: 396 IINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPE 455
           I++E+LR+YP  PL+VPH +S+DC VGGYH+PRGTML  N WAIH DPK W +P  F PE
Sbjct: 370 IVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPE 429

Query: 456 RFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTE- 515
           RFE+   E     L+ FG GRR CPG GL  R+  L +GSLIQCFEWER GEE VDMTE 
Sbjct: 430 RFEK---EGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEG 489

Query: 516 GIAVSMPKAQRLLAKCTPRPI 531
           G  V MPKA  L+A C  RP+
Sbjct: 490 GGGVIMPKAIPLVAMCKARPV 491

BLAST of Spg016936 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 463.0 bits (1190), Expect = 3.3e-130
Identity = 239/477 (50.10%), Postives = 322/477 (67.51%), Query Frame = 0

Query: 58  NLPPTPFPSVPVIGHLHLLKKPLYRTLAKISN--RYGPVVFLRFGSRQVLVVSSPSAAEE 117
           NLPP+P  S+PVIGHL LLK P++RT   +S      P+  LR G+R V V SS S AEE
Sbjct: 29  NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEE 88

Query: 118 CLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLRRISTVEVLSTHRLQMLSTV 177
           C TKND+V ANRP  +L+K   Y+ T ++ +SYGDHWRNLRRI +VE+ S HRL    ++
Sbjct: 89  CFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSI 148

Query: 178 RLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIMLRMIVGKRFYGDDVDDADEARCY 237
           R +E+  L+ RL+R+ +Q     +MK+   DLT+N +LRM+ GKR+YGD V+D  EA+  
Sbjct: 149 RKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRV 208

Query: 238 RELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQ 297
           R+L A+    +G  N  D++P++  +   S  E  + +   R D F+Q L+D++R+    
Sbjct: 209 RQLIADVVACAGAGNAVDYLPVLRLV---SDYETRVKKLAGRLDEFLQGLVDEKRE---A 268

Query: 298 TETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLVLLAAGTETSVNTMEWALSL 357
            E G+        TMI+ LL LQESQP+ + D  IK  ML L+ AGT+TS  T+EWALS 
Sbjct: 269 KEKGN--------TMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSN 328

Query: 358 MLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGIINETLRMYPPGPLMVPHESSK 417
           +LN P+VL KA+ EID ++G DRL+DESD++NLPYL+ I++ETLR+YP  P+++PH +S+
Sbjct: 329 VLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASE 388

Query: 418 DCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFERVESEKYGFNLMPFGAGRR 477
           DC V GY +PRGT+L  N+WAIH DP+ W +P  F PERFE+   E     LMPFG GRR
Sbjct: 389 DCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK---EGEAQKLMPFGLGRR 448

Query: 478 GCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGIAVSMPKAQRLLAKCTPRP 530
            CPG GL  R+I L +GSLIQC EWE+ GEE VDM+EG  V+MPKA+ L A C  RP
Sbjct: 449 ACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMCRARP 487

BLAST of Spg016936 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 459.1 bits (1180), Expect = 4.8e-129
Identity = 237/505 (46.93%), Postives = 329/505 (65.15%), Query Frame = 0

Query: 31  VLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNR 90
           +L  +FL I + L+L     +      NLPP+P   +PVIGHLHLLK+P++RT   IS  
Sbjct: 8   LLSFLFLTISIKLLLTKSNRK-----PNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKS 67

Query: 91  YG--PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 150
            G  P+  LR G+R V V+SS S AEEC TKND+V ANRP ++++K  GYN TN++ +SY
Sbjct: 68  LGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASY 127

Query: 151 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLT 210
           GDHWRNLRRI+ VE+ S+HR+   S++R +E+  LI  L+R          +K+   +L 
Sbjct: 128 GDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLA 187

Query: 211 YNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVE 270
           +N ++ M+ GKR+YG   +D DEA+  REL AE    +G  N+ D++P ++W+   +  E
Sbjct: 188 FNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFE 247

Query: 271 NEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDE 330
           N+      R D  +Q L+D++R    + E G        +T+I+ LL  QE++PE Y D 
Sbjct: 248 NQTKILGNRLDRVLQKLVDEKR---AEKEKG--------QTLIDHLLSFQETEPEYYTDV 307

Query: 331 TIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANL 390
            IK ++L L+ AGT+TS  T+EWA+S +LN PE+L+KA+ EID ++G DRL++ESD+ NL
Sbjct: 308 IIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNL 367

Query: 391 PYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPR 450
            YL+ I++ETLR+YP  PL++PH SS +C V GY +PR T+L  N+WA+H DP  W EP 
Sbjct: 368 HYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPE 427

Query: 451 KFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELV 510
           +F PERFE+   E     LMPFG GRR CPG  LG R++ L +G LIQ FEWER G ELV
Sbjct: 428 RFKPERFEK---EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELV 487

Query: 511 DMTEGIAVSMPKAQRLLAKCTPRPI 531
           DMTEG  ++MPKA  L A C  R I
Sbjct: 488 DMTEGEGITMPKATPLRAMCKARAI 490

BLAST of Spg016936 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 459.1 bits (1180), Expect = 4.8e-129
Identity = 246/502 (49.00%), Postives = 324/502 (64.54%), Query Frame = 0

Query: 42  FLVLYTLTEQFLHKIR---NLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG--PVVF 101
           FL L+TL    L + +   NLPP+P  S+PVIGHLHLLK PL+RT   +S   G  PV  
Sbjct: 11  FLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFH 70

Query: 102 LRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLR 161
           LR G+R V V+SS S AEEC TKND+V ANRP+  +SK  GYN T L+ +SYGDHWRNLR
Sbjct: 71  LRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLR 130

Query: 162 RISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIMLRMI 221
           RI+ VE+ STHRL     +R +E+  LI  L+R          MK    +L  N  +RM+
Sbjct: 131 RIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRML 190

Query: 222 VGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHK 281
            GKR++G+D DD   A+  + L +E+   +G  N  D++ ++ W+   S+ E  I     
Sbjct: 191 AGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYEKRIKNLGN 250

Query: 282 RRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLV 341
           R D F+Q L+D++R    + E G+        TMI+ LL LQ+ QP+ Y D  IK ++L 
Sbjct: 251 RFDTFLQKLVDEKR---AEKEKGE--------TMIDHLLALQDIQPDYYTDVIIKGIILT 310

Query: 342 LLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGIIN 401
           L+ AGT+TS  T+EWA+S +LN PE+LKKA++EID +VG DRL+DESD+ NL YL+ I+ 
Sbjct: 311 LIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVL 370

Query: 402 ETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFE 461
           ETLRMYP  PL++PH SS+DC VGGY IP GTM+  N WA+H DP+ W +P  F PERFE
Sbjct: 371 ETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFE 430

Query: 462 RV-ESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGIA 521
           +  E+EK    L+ FG GRR CPG GL  R+I   +GSL+QCFEWER GE+ VDMTE   
Sbjct: 431 KEGEAEK----LISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKG 490

Query: 522 VSMPKAQRLLAKCTPRPIAEHL 535
            ++PKA  L A C  R I + L
Sbjct: 491 ATLPKAIPLRAMCKARSIVDKL 491

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022940352.13.7e-24880.54cytochrome P450 81D1-like isoform X1 [Cucurbita moschata][more]
XP_022982225.11.4e-24780.35cytochrome P450 81D1-like isoform X1 [Cucurbita maxima][more]
XP_023523281.12.2e-24579.77cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022146589.12.0e-24178.79cytochrome P450 81D1-like [Momordica charantia][more]
XP_008453068.18.6e-23777.13PREDICTED: cytochrome P450 81D1-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
W8JMU71.4e-13349.80Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1[more]
Q6WNQ91.2e-12747.24Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... [more]
Q6WNQ81.3e-12647.60Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1[more]
Q9FG651.7e-12648.12Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
O657901.2e-12445.54Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FJC91.8e-24880.54cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114459... [more]
A0A6J1J2286.8e-24880.35cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119... [more]
A0A6J1CYI59.5e-24278.79cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV... [more]
A0A1S3BUR64.1e-23777.13cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1[more]
A0A0A0L2I73.9e-23575.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37360.17.2e-13348.81cytochrome P450, family 81, subfamily D, polypeptide 2 [more]
AT4G37340.18.0e-13249.50cytochrome P450, family 81, subfamily D, polypeptide 3 [more]
AT4G37370.13.3e-13050.10cytochrome P450, family 81, subfamily D, polypeptide 8 [more]
AT4G37320.14.8e-12946.93cytochrome P450, family 81, subfamily D, polypeptide 5 [more]
AT4G37330.14.8e-12949.00cytochrome P450, family 81, subfamily D, polypeptide 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 180..200
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 29..498
e-value: 8.6E-20
score: 68.9
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 42..534
NoneNo IPR availablePANTHERPTHR47947:SF24CYTOCHROME P450 81E8coord: 42..534
IPR001128Cytochrome P450PRINTSPR00385P450coord: 390..401
score: 40.67
coord: 337..354
score: 34.25
coord: 465..474
score: 59.44
coord: 474..485
score: 31.43
IPR001128Cytochrome P450PFAMPF00067p450coord: 61..517
e-value: 9.6E-97
score: 324.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 326..343
score: 31.68
coord: 430..454
score: 39.4
coord: 88..107
score: 37.31
coord: 389..407
score: 31.91
coord: 204..222
score: 28.06
coord: 474..497
score: 33.94
coord: 464..474
score: 57.89
coord: 112..133
score: 27.91
coord: 346..372
score: 37.13
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 54..534
e-value: 3.0E-125
score: 420.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 60..528
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 467..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016936.1Spg016936.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen