Homology
BLAST of Spg016936 vs. NCBI nr
Match:
XP_022940352.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 868.2 bits (2242), Expect = 3.7e-248
Identity = 414/514 (80.54%), Postives = 471/514 (91.63%), Query Frame = 0
Query: 32 LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
+GAI +YIPLF VLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1 MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60
Query: 92 GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61 GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120
Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLA+ E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLR 180
Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
MIVGKRFYG+DV+DADEA +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMEC 240
Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
H++RDAFMQ+LI+Q + R ++TE +SFRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300
Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360
Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420
Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
F +VESEK GF LMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGEELVDMTEG+
Sbjct: 421 FVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGV 480
Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514
BLAST of Spg016936 vs. NCBI nr
Match:
XP_022982225.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 866.3 bits (2237), Expect = 1.4e-247
Identity = 413/514 (80.35%), Postives = 471/514 (91.63%), Query Frame = 0
Query: 32 LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
+GAI LYIPLFLVLY LTEQF+HKIRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1 MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60
Query: 92 GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61 GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120
Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLAR E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180
Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
MIVGKRFYG+DV+DADEA +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMEC 240
Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
H++RDAFMQ+LI+Q + R ++TE + FRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300
Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360
Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420
Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
F +V+SEK GF L+PFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480
Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514
BLAST of Spg016936 vs. NCBI nr
Match:
XP_023523281.1 (cytochrome P450 81D1-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 859.0 bits (2218), Expect = 2.2e-245
Identity = 410/514 (79.77%), Postives = 469/514 (91.25%), Query Frame = 0
Query: 32 LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
+GAI +YIPLFLVLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1 MGAIIVYIPLFLVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60
Query: 92 GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61 GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120
Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
WRNLRRISTVE+LSTHRLQML+ VR +EV SLI RLAR E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRSDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180
Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
MIVGKRFYG+DV+DADEA +RELQ++ST+L GK+N+ DFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMEDFIPLMSWLGLGWAAEKEIMEC 240
Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
H++RDAFMQ+LI+Q + R ++TE +SFRDGRKKT+IEVL+ELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLVELQESDPDQYNDETIRALM 300
Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360
Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
INETLRMYPP PLMVPHESSKDCSVGGY IPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYQIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420
Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
F +V+SEK+GF LMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKHGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480
Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
AVSMPKA+ L AKC PRPI L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVARLHLSQTKSNLAY 514
BLAST of Spg016936 vs. NCBI nr
Match:
XP_022146589.1 (cytochrome P450 81D1-like [Momordica charantia])
HSP 1 Score: 845.9 bits (2184), Expect = 2.0e-241
Identity = 416/528 (78.79%), Postives = 474/528 (89.77%), Query Frame = 0
Query: 26 SEAMGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLA 85
S+ + +GAIFLY PLFLVLY LTE FLHKIRNLPPTPFPS+P+IGHL+LL KP+YRTLA
Sbjct: 3 SKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLA 62
Query: 86 KISNRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVW 145
ISNR+GPVVFLRFGSR VLVVSSPS AEECLTKND+VFANRPRLL+SK FGYNNTNLVW
Sbjct: 63 DISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVW 122
Query: 146 SSYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQ--VHNMKNAFFD 205
SSYGDHWR+LRRISTVEVLSTHRLQ+LSTVRLEEV SLIR+LA+SE Q + M+++FFD
Sbjct: 123 SSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFD 182
Query: 206 LTYNIMLRMIVGKRFYGDDVDDADE-ARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGS 265
LT+N+MLRMI GKRFYGDDVDDADE A+ +RELQAESTRL+G SNIGDF+P+M+WLGFG
Sbjct: 183 LTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 242
Query: 266 AVENEIMECHKRRDAFMQSLIDQQRKRM---VQTETGDSFRDGRKKTMIEVLLELQESQP 325
+VE ++M+CH +RDAFMQ+LI++QRKR ++E DSFRDGR+KTMIEVLLELQES+P
Sbjct: 243 SVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEP 302
Query: 326 EQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDE 385
+ Y DE+IKALMLVLL AGTETSVNTMEWALSL+LN PEVLKKAQ+EID QVGQDRLIDE
Sbjct: 303 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDBQVGQDRLIDE 362
Query: 386 SDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPK 445
SD+A+LPYLRGII ET RMYPP PLMVPH+SS+DCSVGG+HIPRGTMLYVNLWAIHNDPK
Sbjct: 363 SDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPK 422
Query: 446 NWGEPRKFDPERFERVESEK--YGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEW 505
W P KFDP+RF+R+ES+K YGFNLMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEW
Sbjct: 423 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 482
Query: 506 ERPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
ERPGE+LVDMTEGIAVSMPKAQ L AKC PRPI +L S T++N AH
Sbjct: 483 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529
BLAST of Spg016936 vs. NCBI nr
Match:
XP_008453068.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])
HSP 1 Score: 830.5 bits (2144), Expect = 8.6e-237
Identity = 398/516 (77.13%), Postives = 450/516 (87.21%), Query Frame = 0
Query: 29 MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
MGVLG+IF+Y PLFLVLY L E LHKIRNLPPTPFP +P+IGHLHLLKKP+YRTLAKIS
Sbjct: 1 MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60
Query: 89 NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 148
N YGPVV+ RFGSR+VLVVSS AAEECLTKND+VFANRPRL++SKWFGYNNTNL+WSSY
Sbjct: 61 NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120
Query: 149 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 208
GDHWRNLRRIST+E+LSTHRLQM S+VRLEEV SLIRRLA E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180
Query: 209 MLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 268
MLRM+VGKR+YG+DVDD DEA+ +R+LQ + +L GKS + DFIPL+SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240
Query: 269 MECHKRRDAFMQSLIDQQRKR-MVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 328
+E H +RD FMQ+LIDQ +KR Q + ++DGR+KTMIEVLLELQ+ +P QY DETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300
Query: 329 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPY 388
+ALML+LLAAGTETSV TMEWALSLMLN PE LKK Q EID QVG DRLIDESD+ANLP
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360
Query: 389 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 448
LRGIINET RMYPP PLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHNDPK W PRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420
Query: 449 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDM 508
+P RFE +ESEK+GFNLMPFG+GRRGCPGEGLGLR+IGLV+GSL+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480
Query: 509 TEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNP 544
TEGIA++MPKA L AKCTPRPI L LS SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516
BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 478.0 bits (1229), Expect = 1.4e-133
Identity = 249/500 (49.80%), Postives = 333/500 (66.60%), Query Frame = 0
Query: 37 LYIPLFLVLYTLTEQFL---HKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGP 96
LY L +VL +++ + + RNLPP+P ++PVIGHLHL+ K L+R+L +S +YG
Sbjct: 7 LYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGS 66
Query: 97 VVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWR 156
V L+ G+R VLVVSSP+AAEEC TKNDIVFANRP +L K+ GYN T +V S YG+HWR
Sbjct: 67 VFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWR 126
Query: 157 NLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIML 216
NLRR++ VE+ S L ++R +EV L+ L +S Q MK+ +L++N+ +
Sbjct: 127 NLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTM 186
Query: 217 RMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIME 276
RM+ GKR++G DV D+DEA+ +R L E +G SN GDF+P + W+ F + E ++ +
Sbjct: 187 RMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKN-YEKKVSK 246
Query: 277 CHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKAL 336
+ DAF+Q LI + R + TMI+ LL LQESQPE Y D+ IK +
Sbjct: 247 ISQEMDAFLQRLIHESR------------INKNNVTMIDHLLSLQESQPEYYTDQIIKGI 306
Query: 337 MLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRG 396
++VLL AGT+TS T+EWA+SL+LN PE L+KA+ EI+ QVG +RLI+E D+ L YL
Sbjct: 307 IMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHN 366
Query: 397 IINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPE 456
II+ET R+ P P++VPHESS DC V GY +P+GT+L VN WAIH DP+ W EP F PE
Sbjct: 367 IISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPE 426
Query: 457 RFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEG 516
R VE E LMPFG GRR CPG GL R++GL +G+LIQCFEW+R GE +DM EG
Sbjct: 427 RHGGVELEP--SKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEG 486
Query: 517 IAVSMPKAQRLLAKCTPRPI 531
++MPKAQ L A C PR I
Sbjct: 487 SGLTMPKAQPLEALCKPRNI 490
BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 240/508 (47.24%), Postives = 335/508 (65.94%), Query Frame = 0
Query: 38 YIPLFLVLYTLTEQFLHKI-----RNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG 97
Y L + + +T + L KI +NLPP P P++P+IG+LH LK PL+RT +S YG
Sbjct: 4 YYSLLSLSFIITIKILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYG 63
Query: 98 PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHW 157
+ L FGSR V+VVSSPS A EC TKNDI+ ANRPR L K+ YN T L +SYGDHW
Sbjct: 64 DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 123
Query: 158 RNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLAR---SE----TQVHNMKNAFFDLT 217
RNLRRI+T++VLS +RL VR +E N LI++L + SE T+V ++ ++T
Sbjct: 124 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKV-ELRPRLTEMT 183
Query: 218 YNIMLRMIVGKRFYGD--DVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSA 277
+N M+RMI GKR+YGD DV D +EA+ +RE+ +E L G +N GDF+PL+ + +
Sbjct: 184 FNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN- 243
Query: 278 VENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYN 337
+E KR +AF++ LI++ R+ + ++ G TMI+ LL+L ESQPE Y+
Sbjct: 244 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGG---------TMIDHLLKLSESQPEYYS 303
Query: 338 DETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVA 397
D IK L+ +L AGT+TS T+EW +S +LN PEVLKKA+ E+D Q+G+++L+DE D++
Sbjct: 304 DHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLS 363
Query: 398 NLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGE 457
LPYL+ II+ETLR++PP PL++PH SS+DC++G +++P+ T++ N+W IH DPK+W +
Sbjct: 364 KLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWND 423
Query: 458 PRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEE 517
F PERFE+ E +M FG GRR CPG L R +G +G LIQCFEWER EE
Sbjct: 424 ALSFKPERFEKEEEVN---KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEE 483
Query: 518 LVDMTEGIAVSMPKAQRLLAKCTPRPIA 532
+DM EG ++MP L A C PIA
Sbjct: 484 KLDMMEGKGITMPMKIPLRAMCKALPIA 496
BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 454.9 bits (1169), Expect = 1.3e-126
Identity = 238/500 (47.60%), Postives = 322/500 (64.40%), Query Frame = 0
Query: 37 LYIPLFLVLYTLTEQF--LHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYGPV 96
L I LF ++ TL F K +NLPP P +P+IG+LH LK+PL+ T +S +YG +
Sbjct: 8 LIISLFFLIITLKVFFNTSRKFKNLPPGP-QCLPIIGNLHQLKQPLHHTFHTLSQKYGQI 67
Query: 97 VFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRN 156
L FGSR V+VVSS + A+EC TKNDIV ANRP L K+ GYNNT + S YGDHWRN
Sbjct: 68 FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 127
Query: 157 LRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSE----TQVHNMKNAFFDLTYNIML 216
LRRI ++E+LS+HRL +R +E+ LI++LA+ T+V ++ F ++T+N ++
Sbjct: 128 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEV-ELRPMFSEMTFNTIM 187
Query: 217 RMIVGKRFYGDDVD--DADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 276
RM+ GKR+YG+D D D +EAR +R + E L G +N+GDF+ + W F +E +
Sbjct: 188 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDF-DGLEKRL 247
Query: 277 MECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIK 336
+ KR DAF+Q LID+ R F TMI+ LL Q+SQPE Y D+ IK
Sbjct: 248 KKISKRTDAFLQGLIDEHR-----------FGKRNSNTMIDHLLTQQQSQPEYYTDQIIK 307
Query: 337 ALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYL 396
LM+V+L AGT+TS T+EWA+S +LN PE++KKA+ E+D +G DR +DE D++ LPYL
Sbjct: 308 GLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYL 367
Query: 397 RGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFD 456
+ I+ ETLR++ PL+VPH SS+D S+GGY+IP+ T+L VN W IH DP W +P F
Sbjct: 368 QSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFK 427
Query: 457 PERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMT 516
PERFE+ E L+ FG GRR CPGE L R GL +G LIQCFEW+R GEE +DM
Sbjct: 428 PERFEK---EGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMV 487
Query: 517 EGIAVSMPKAQRLLAKCTPR 529
E ++ K L A C R
Sbjct: 488 EAKGITAGKKTSLNAMCKVR 490
BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 454.5 bits (1168), Expect = 1.7e-126
Identity = 243/505 (48.12%), Postives = 329/505 (65.15%), Query Frame = 0
Query: 35 IFLYIPLFLVLYTLTEQFLH-KIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS----- 94
+ LY L+ ++ +FL K +NLPP+P +P+IGHL LLK P++RTL S
Sbjct: 8 VVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDH 67
Query: 95 NRYGPVVFLRFGSRQVLVVSSPS-AAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSS 154
N G V+ LR GSR V VVSS AAEEC KND+V ANRP++++ K GYNNTN++ +
Sbjct: 68 NDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAP 127
Query: 155 YGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLAR---SETQVHNMKNAFFDL 214
YGDHWRNLRR+ T+E+ STHRL VR +EV LI RL+R ++ V +K DL
Sbjct: 128 YGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDL 187
Query: 215 TYNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAV 274
T+N ++RM+ GKR+YG++ D +EA+ R+L A+ + N D++P+ L S+
Sbjct: 188 TFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPI---LRLFSSY 247
Query: 275 ENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYND 334
EN + + + D F+Q LID +R Q ETG TMI+ LL LQ+S E Y D
Sbjct: 248 ENRVKKLGEETDKFLQGLIDDKRG---QQETG--------TTMIDHLLVLQKSDIEYYTD 307
Query: 335 ETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVAN 394
+ IK ++L+++ AGT TS T+EWALS +LN P+V+ KA+ EID +VG DRLI+E+D++
Sbjct: 308 QIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSE 367
Query: 395 LPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEP 454
LPYL+ I+ ETLR++P PL+VPH +S+DC +G Y +PRGT L VN WAIH DP W +P
Sbjct: 368 LPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDP 427
Query: 455 RKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEEL 514
F PERFE+ E + L+ FG GRR CPG GL R++GL +GSLIQCFEWER G
Sbjct: 428 DSFKPERFEKEEEAQ---KLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVE 487
Query: 515 VDMTEGIAVSMPKAQRLLAKCTPRP 530
VDM EG+ ++PKA L A C RP
Sbjct: 488 VDMKEGVGNTVPKAIPLKAICKARP 495
BLAST of Spg016936 vs. ExPASy Swiss-Prot
Match:
O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)
HSP 1 Score: 448.4 bits (1152), Expect = 1.2e-124
Identity = 235/516 (45.54%), Postives = 334/516 (64.73%), Query Frame = 0
Query: 36 FLYIP-LFLV-----LYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISN 95
F+ +P LFLV LY+ T++F NLPP P PS P +GHLHL+K P++R L + SN
Sbjct: 4 FILLPLLFLVISYKFLYSKTQRF-----NLPPGP-PSRPFVGHLHLMKPPIHRLLQRYSN 63
Query: 96 RYGPVVFLRFGSRQVLVVSSPSAAEECLT-KNDIVFANRPRLLLSKWFGYNNTNLVWSSY 155
+YGP+ LRFGSR+V+V++SPS A+E T +NDIV ++RP L +K+ YN+T + + Y
Sbjct: 64 QYGPIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPY 123
Query: 156 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAF------- 215
GDHWRNLRRI + E+LS+HRL +R +E+ ++ RL+R TQ N N F
Sbjct: 124 GDHWRNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSR-YTQTSNESNDFTHIELEP 183
Query: 216 --FDLTYNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLG 275
DLT+N ++RM+ GKR+YGDDV++ +EA +++L + SG ++ D++P++
Sbjct: 184 LLSDLTFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKL-- 243
Query: 276 FGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQP 335
FG+ E E+ K D +Q L+D+ R RD TM+ L+ LQ+ QP
Sbjct: 244 FGNKFEKEVKAIGKSMDDILQRLLDECR------------RDKEGNTMVNHLISLQQQQP 303
Query: 336 EQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDE 395
E Y D IK LM+ ++ AGTETS T+EWA++ +L PEVL+KA+ EID ++G+DRLIDE
Sbjct: 304 EYYTDVIIKGLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDE 363
Query: 396 SDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPK 455
SD+A LPYL+ +++ET R++P P ++P + D +GGY +PR T++ VN WAIH DP+
Sbjct: 364 SDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPE 423
Query: 456 NWGEPRKFDPERF-ERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWE 515
W EP KF+P+R+ + S+ Y + LMPFG GRR CPG GLG R++ L +GSLIQCFEWE
Sbjct: 424 IWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWE 483
Query: 516 RPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIAEHL 535
E +DM+E + M K L A C PRPI L
Sbjct: 484 NVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSKL 498
BLAST of Spg016936 vs. ExPASy TrEMBL
Match:
A0A6J1FJC9 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445994 PE=3 SV=1)
HSP 1 Score: 868.2 bits (2242), Expect = 1.8e-248
Identity = 414/514 (80.54%), Postives = 471/514 (91.63%), Query Frame = 0
Query: 32 LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
+GAI +YIPLF VLY LTEQFLH+IRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1 MGAIIVYIPLFFVLYVLTEQFLHRIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60
Query: 92 GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61 GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120
Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLA+ E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAAVRLDEVKSLIGRLAKGENQVQDMNNAFFDLTFNVMLR 180
Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
MIVGKRFYG+DV+DADEA +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGWAAEKEIMEC 240
Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
H++RDAFMQ+LI+Q + R ++TE +SFRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPDNSFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300
Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360
Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420
Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
F +VESEK GF LMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGEELVDMTEG+
Sbjct: 421 FVKVESEKLGFKLMPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEELVDMTEGV 480
Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514
BLAST of Spg016936 vs. ExPASy TrEMBL
Match:
A0A6J1J228 (cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119 PE=3 SV=1)
HSP 1 Score: 866.3 bits (2237), Expect = 6.8e-248
Identity = 413/514 (80.35%), Postives = 471/514 (91.63%), Query Frame = 0
Query: 32 LGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRY 91
+GAI LYIPLFLVLY LTEQF+HKIRNLPPTPF S+P+IGHLHLLKKP+YRTLA+ISNRY
Sbjct: 1 MGAIILYIPLFLVLYVLTEQFIHKIRNLPPTPFLSLPIIGHLHLLKKPMYRTLAEISNRY 60
Query: 92 GPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDH 151
GPVVFLRFGSR VLVVSSPSAAEECL+KND+VFA+RPRLL+SKWFGYNN NLVWSSYGDH
Sbjct: 61 GPVVFLRFGSRGVLVVSSPSAAEECLSKNDVVFADRPRLLISKWFGYNNRNLVWSSYGDH 120
Query: 152 WRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNIMLR 211
WRNLRRISTVE+LSTHRLQML+ VRL+EV SLI RLAR E QV +M NAFFDLT+N+MLR
Sbjct: 121 WRNLRRISTVEILSTHRLQMLAGVRLDEVKSLIGRLARGENQVQDMNNAFFDLTFNVMLR 180
Query: 212 MIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMEC 271
MIVGKRFYG+DV+DADEA +RELQ++ST+L GK+N+GDFIPLMSWLG G A E EIMEC
Sbjct: 181 MIVGKRFYGEDVEDADEAHRFRELQSKSTQLGGKTNMGDFIPLMSWLGLGRAAEKEIMEC 240
Query: 272 HKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALM 331
H++RDAFMQ+LI+Q + R ++TE + FRDGRKKT+IEVLLELQES P+QYNDETI+ALM
Sbjct: 241 HQKRDAFMQNLINQHKNRAIETEPHNLFRDGRKKTLIEVLLELQESDPDQYNDETIRALM 300
Query: 332 LVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGI 391
LVLLAAGTETS+NTMEWALSLMLN PEVL+KAQ+EID +VG DRL+DESD+AN+P+LRGI
Sbjct: 301 LVLLAAGTETSMNTMEWALSLMLNNPEVLQKAQLEIDQKVGHDRLMDESDMANVPFLRGI 360
Query: 392 INETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPER 451
INETLRMYPP PLMVPHESSKDCSVGGYHIPRGTML+VNLWAIHNDPK W +PRKFDP+R
Sbjct: 361 INETLRMYPPAPLMVPHESSKDCSVGGYHIPRGTMLFVNLWAIHNDPKIWADPRKFDPDR 420
Query: 452 FERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGI 511
F +V+SEK GF L+PFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEWERPGE+LVDMTEG+
Sbjct: 421 FVKVDSEKLGFKLLPFGSGRRGCPGEGLGLRVIGLVLGSLIQCFEWERPGEDLVDMTEGV 480
Query: 512 AVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
AVSMPKA+ L AKC PRPI + L LS T+SN A+
Sbjct: 481 AVSMPKARHLQAKCKPRPIVDRLHLSQTKSNLAY 514
BLAST of Spg016936 vs. ExPASy TrEMBL
Match:
A0A6J1CYI5 (cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV=1)
HSP 1 Score: 845.9 bits (2184), Expect = 9.5e-242
Identity = 416/528 (78.79%), Postives = 474/528 (89.77%), Query Frame = 0
Query: 26 SEAMGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLA 85
S+ + +GAIFLY PLFLVLY LTE FLHKIRNLPPTPFPS+P+IGHL+LL KP+YRTLA
Sbjct: 3 SKILAAMGAIFLYFPLFLVLYILTEHFLHKIRNLPPTPFPSLPLIGHLYLLNKPIYRTLA 62
Query: 86 KISNRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVW 145
ISNR+GPVVFLRFGSR VLVVSSPS AEECLTKND+VFANRPRLL+SK FGYNNTNLVW
Sbjct: 63 DISNRHGPVVFLRFGSRPVLVVSSPSVAEECLTKNDVVFANRPRLLISKCFGYNNTNLVW 122
Query: 146 SSYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQ--VHNMKNAFFD 205
SSYGDHWR+LRRISTVEVLSTHRLQ+LSTVRLEEV SLIR+LA+SE Q + M+++FFD
Sbjct: 123 SSYGDHWRSLRRISTVEVLSTHRLQILSTVRLEEVRSLIRKLAKSENQDKILTMRHSFFD 182
Query: 206 LTYNIMLRMIVGKRFYGDDVDDADE-ARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGS 265
LT+N+MLRMI GKRFYGDDVDDADE A+ +RELQAESTRL+G SNIGDF+P+M+WLGFG
Sbjct: 183 LTFNVMLRMIAGKRFYGDDVDDADEDAKIFRELQAESTRLTGISNIGDFVPMMTWLGFGW 242
Query: 266 AVENEIMECHKRRDAFMQSLIDQQRKRM---VQTETGDSFRDGRKKTMIEVLLELQESQP 325
+VE ++M+CH +RDAFMQ+LI++QRKR ++E DSFRDGR+KTMIEVLLELQES+P
Sbjct: 243 SVEKKMMDCHYKRDAFMQTLIEEQRKRSTAETESEIDDSFRDGRRKTMIEVLLELQESEP 302
Query: 326 EQYNDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDE 385
+ Y DE+IKALMLVLL AGTETSVNTMEWALSL+LN PEVLKKAQ+EID QVGQDRLIDE
Sbjct: 303 DHYTDESIKALMLVLLTAGTETSVNTMEWALSLLLNHPEVLKKAQVEIDDQVGQDRLIDE 362
Query: 386 SDVANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPK 445
SD+A+LPYLRGII ET RMYPP PLMVPH+SS+DCSVGG+HIPRGTMLYVNLWAIHNDPK
Sbjct: 363 SDMAHLPYLRGIIXETFRMYPPAPLMVPHQSSEDCSVGGHHIPRGTMLYVNLWAIHNDPK 422
Query: 446 NWGEPRKFDPERFERVESEK--YGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEW 505
W P KFDP+RF+R+ES+K YGFNLMPFG+GRRGCPGEGLGLR+IGLV+GSLIQCFEW
Sbjct: 423 IWANPTKFDPDRFDRLESDKYGYGFNLMPFGSGRRGCPGEGLGLRIIGLVLGSLIQCFEW 482
Query: 506 ERPGEELVDMTEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNPAH 546
ERPGE+LVDMTEGIAVSMPKAQ L AKC PRPI +L S T++N AH
Sbjct: 483 ERPGEKLVDMTEGIAVSMPKAQPLQAKCRPRPIIANLP-SQTKTNQAH 529
BLAST of Spg016936 vs. ExPASy TrEMBL
Match:
A0A1S3BUR6 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1)
HSP 1 Score: 830.5 bits (2144), Expect = 4.1e-237
Identity = 398/516 (77.13%), Postives = 450/516 (87.21%), Query Frame = 0
Query: 29 MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
MGVLG+IF+Y PLFLVLY L E LHKIRNLPPTPFP +P+IGHLHLLKKP+YRTLAKIS
Sbjct: 1 MGVLGSIFVYFPLFLVLYILIEHLLHKIRNLPPTPFPLLPIIGHLHLLKKPIYRTLAKIS 60
Query: 89 NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 148
N YGPVV+ RFGSR+VLVVSS AAEECLTKND+VFANRPRL++SKWFGYNNTNL+WSSY
Sbjct: 61 NHYGPVVYFRFGSRKVLVVSSSFAAEECLTKNDVVFANRPRLIISKWFGYNNTNLIWSSY 120
Query: 149 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 208
GDHWRNLRRIST+E+LSTHRLQM S+VRLEEV SLIRRLA E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRLQMFSSVRLEEVKSLIRRLANDENQIHNMKNEFFDLTYNM 180
Query: 209 MLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 268
MLRM+VGKR+YG+DVDD DEA+ +R+LQ + +L GKS + DFIPL+SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRYYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPLVSWMGFGSTLENKI 240
Query: 269 MECHKRRDAFMQSLIDQQRKR-MVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 328
+E H +RD FMQ+LIDQ +KR Q + ++DGR+KTMIEVLLELQ+ +P QY DETI
Sbjct: 241 IELHVKRDTFMQNLIDQHKKRNFDQNNSNKIYQDGRRKTMIEVLLELQQYEPNQYTDETI 300
Query: 329 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPY 388
+ALML+LLAAGTETSV TMEWALSLMLN PE LKK Q EID QVG DRLIDESD+ANLP
Sbjct: 301 RALMLILLAAGTETSVVTMEWALSLMLNHPEFLKKLQNEIDSQVGHDRLIDESDMANLPS 360
Query: 389 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 448
LRGIINET RMYPP PLMVPHESSKDC VGGYHIPRGT+LYVNLWAIHNDPK W PRKF
Sbjct: 361 LRGIINETFRMYPPAPLMVPHESSKDCIVGGYHIPRGTILYVNLWAIHNDPKIWDNPRKF 420
Query: 449 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDM 508
+P RFE +ESEK+GFNLMPFG+GRRGCPGEGLGLR+IGLV+GSL+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLESEKFGFNLMPFGSGRRGCPGEGLGLRVIGLVLGSLVQCFEWERPGEELVDM 480
Query: 509 TEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNP 544
TEGIA++MPKA L AKCTPRPI L LS SNP
Sbjct: 481 TEGIALTMPKAHCLQAKCTPRPIVHRLHLSHKISNP 516
BLAST of Spg016936 vs. ExPASy TrEMBL
Match:
A0A0A0L2I7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1)
HSP 1 Score: 823.9 bits (2127), Expect = 3.9e-235
Identity = 391/516 (75.78%), Postives = 452/516 (87.60%), Query Frame = 0
Query: 29 MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
MGVL +IF+YIPLF VLYTL E +HKIRNLPPTPFP +PVIGHLHLLK P+YRTLA+IS
Sbjct: 1 MGVLESIFVYIPLFFVLYTLIEYLIHKIRNLPPTPFPLLPVIGHLHLLKNPIYRTLAEIS 60
Query: 89 NRYGPVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 148
N YGPVV+ RFG R+VLVVSSP AAEECLTKNDIVFANRPRL++SKWFGYNNTNLVWSSY
Sbjct: 61 NHYGPVVYFRFGQRRVLVVSSPFAAEECLTKNDIVFANRPRLIISKWFGYNNTNLVWSSY 120
Query: 149 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVHNMKNAFFDLTYNI 208
GDHWRNLRRIST+E+LSTHR+QMLS RLEEV SLIRRLA +E Q+HNMKN FFDLTYN+
Sbjct: 121 GDHWRNLRRISTIEILSTHRIQMLSMFRLEEVKSLIRRLANNENQIHNMKNEFFDLTYNV 180
Query: 209 MLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEI 268
MLRM+VGKRFYG+DVDD DEA+ +R+LQ + +L GKS + DFIP +SW+GFGS +EN+I
Sbjct: 181 MLRMLVGKRFYGEDVDDVDEAKIFRQLQIDLGQLGGKSILQDFIPFVSWMGFGSTIENKI 240
Query: 269 MECHKRRDAFMQSLIDQQRKRMV-QTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETI 328
+ECH +RD FMQ+LIDQ +KR+V Q ++ + +DGR+KTMIEVLLELQ+ P+QY DETI
Sbjct: 241 IECHVKRDTFMQNLIDQHKKRIVDQNKSNNISQDGRRKTMIEVLLELQQYNPDQYTDETI 300
Query: 329 KALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPY 388
+ALMLVLLAAGTETSV MEWALSLMLN P+ LKK Q EID QVG DRLIDESD+ANLP
Sbjct: 301 RALMLVLLAAGTETSVAAMEWALSLMLNHPKFLKKLQNEIDNQVGHDRLIDESDMANLPS 360
Query: 389 LRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKF 448
LRGIINETLRMYPP PL+VPHESSKDC++GGYHIPRGT+L+VNLWAIHNDPK W PRKF
Sbjct: 361 LRGIINETLRMYPPAPLVVPHESSKDCTIGGYHIPRGTILFVNLWAIHNDPKIWDNPRKF 420
Query: 449 DPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDM 508
+P RFE +E+EK+GFNL+PFG+GRRGCPGEGL LR+IGLV+G+L+QCFEWERPGEELVDM
Sbjct: 421 NPNRFESLENEKFGFNLIPFGSGRRGCPGEGLALRVIGLVLGALVQCFEWERPGEELVDM 480
Query: 509 TEGIAVSMPKAQRLLAKCTPRPIAEHLDLSPTRSNP 544
TEG+A++MPKA L AKCTPRPI L LS SNP
Sbjct: 481 TEGVALTMPKAHCLQAKCTPRPIVHRLHLSHKESNP 516
BLAST of Spg016936 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 471.9 bits (1213), Expect = 7.2e-133
Identity = 247/506 (48.81%), Postives = 331/506 (65.42%), Query Frame = 0
Query: 29 MGVLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKIS 88
M L IF + + L L L + K+ NLPP+P ++PVIGHL LLK PL+R +S
Sbjct: 1 MEALMLIFTFCFIVLSLIFLIGRIKRKL-NLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60
Query: 89 NRYG--PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWS 148
G P++ LR G+R + VVSS S AEEC TKND++ ANR + +K Y N+ +V +
Sbjct: 61 QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120
Query: 149 SYGDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSE----TQVHNMKNAFF 208
SY +HWRNLRRI +E+ S HRL S++R +E+ LI RL R+ T+V MK+ F
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKV-EMKSMFS 180
Query: 209 DLTYNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGS 268
DLT+N ++RM+ GK +YGD +D EA+ R L AE+ SG N D+IP+++W+ +
Sbjct: 181 DLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS- 240
Query: 269 AVENEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQY 328
E I + R D F+Q L+D++R+ ++ ++ TM++ LL LQE+QPE Y
Sbjct: 241 --ETRIKKLAGRLDEFLQGLVDEKREG----------KEKKENTMVDHLLCLQETQPEYY 300
Query: 329 NDETIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDV 388
D IK ML L+A GT+T+ T+EWALS +LN PEVL KA+ EID +G DRL++ESD+
Sbjct: 301 MDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDI 360
Query: 389 ANLPYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWG 448
NLPYL+ I++ETLR+YP P+++PH +SKDC VGGY +PRGTML N WAIH DP W
Sbjct: 361 PNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWD 420
Query: 449 EPRKFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGE 508
+P F PERFE+ K LMPFG GRR CPG GL R++ L +GSLIQCFEWER GE
Sbjct: 421 DPTSFKPERFEKEGEAK---KLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGE 480
Query: 509 ELVDMTEGIAVSMPKAQRLLAKCTPR 529
E VDMTEG ++MPKA+ L A C R
Sbjct: 481 EEVDMTEGPGLTMPKARPLEAMCRAR 488
BLAST of Spg016936 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 468.4 bits (1204), Expect = 8.0e-132
Identity = 248/501 (49.50%), Postives = 329/501 (65.67%), Query Frame = 0
Query: 36 FLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG--P 95
FL+I L L T + + NLPP+P ++PVIGHL LLK PL+R +S G P
Sbjct: 10 FLFISLSL---TFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAP 69
Query: 96 VVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWR 155
++ LR G+R V VVSS S AEEC TKND+V ANR L SK Y T +V +SYGDHWR
Sbjct: 70 IISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWR 129
Query: 156 NLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARS---ETQVHNMKNAFFDLTYNIML 215
NLRRI VE+ S HRL S++R +E++ LI L+R+ E MK+ F +LT+N ++
Sbjct: 130 NLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNII 189
Query: 216 RMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIME 275
RM+ GK +YGD +D EA+ REL AE G N D++P+++W+ + E I +
Sbjct: 190 RMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIKK 249
Query: 276 CHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKAL 335
R D F+Q L+D++R+ ++ R+ TM++ LL LQE+QPE Y D IK +
Sbjct: 250 IASRLDEFLQGLVDERREG----------KEKRQNTMVDHLLCLQETQPEYYTDNIIKGI 309
Query: 336 MLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRG 395
ML L+ AGT+TS T+EW LS +LN P++L KA+ EID +VG +RL++ESD+++LPYL+
Sbjct: 310 MLSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQN 369
Query: 396 IINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPE 455
I++E+LR+YP PL+VPH +S+DC VGGYH+PRGTML N WAIH DPK W +P F PE
Sbjct: 370 IVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPE 429
Query: 456 RFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTE- 515
RFE+ E L+ FG GRR CPG GL R+ L +GSLIQCFEWER GEE VDMTE
Sbjct: 430 RFEK---EGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEG 489
Query: 516 GIAVSMPKAQRLLAKCTPRPI 531
G V MPKA L+A C RP+
Sbjct: 490 GGGVIMPKAIPLVAMCKARPV 491
BLAST of Spg016936 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 463.0 bits (1190), Expect = 3.3e-130
Identity = 239/477 (50.10%), Postives = 322/477 (67.51%), Query Frame = 0
Query: 58 NLPPTPFPSVPVIGHLHLLKKPLYRTLAKISN--RYGPVVFLRFGSRQVLVVSSPSAAEE 117
NLPP+P S+PVIGHL LLK P++RT +S P+ LR G+R V V SS S AEE
Sbjct: 29 NLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEE 88
Query: 118 CLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLRRISTVEVLSTHRLQMLSTV 177
C TKND+V ANRP +L+K Y+ T ++ +SYGDHWRNLRRI +VE+ S HRL ++
Sbjct: 89 CFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSI 148
Query: 178 RLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIMLRMIVGKRFYGDDVDDADEARCY 237
R +E+ L+ RL+R+ +Q +MK+ DLT+N +LRM+ GKR+YGD V+D EA+
Sbjct: 149 RKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRV 208
Query: 238 RELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHKRRDAFMQSLIDQQRKRMVQ 297
R+L A+ +G N D++P++ + S E + + R D F+Q L+D++R+
Sbjct: 209 RQLIADVVACAGAGNAVDYLPVLRLV---SDYETRVKKLAGRLDEFLQGLVDEKRE---A 268
Query: 298 TETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLVLLAAGTETSVNTMEWALSL 357
E G+ TMI+ LL LQESQP+ + D IK ML L+ AGT+TS T+EWALS
Sbjct: 269 KEKGN--------TMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSN 328
Query: 358 MLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGIINETLRMYPPGPLMVPHESSK 417
+LN P+VL KA+ EID ++G DRL+DESD++NLPYL+ I++ETLR+YP P+++PH +S+
Sbjct: 329 VLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASE 388
Query: 418 DCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFERVESEKYGFNLMPFGAGRR 477
DC V GY +PRGT+L N+WAIH DP+ W +P F PERFE+ E LMPFG GRR
Sbjct: 389 DCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK---EGEAQKLMPFGLGRR 448
Query: 478 GCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGIAVSMPKAQRLLAKCTPRP 530
CPG GL R+I L +GSLIQC EWE+ GEE VDM+EG V+MPKA+ L A C RP
Sbjct: 449 ACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMCRARP 487
BLAST of Spg016936 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 459.1 bits (1180), Expect = 4.8e-129
Identity = 237/505 (46.93%), Postives = 329/505 (65.15%), Query Frame = 0
Query: 31 VLGAIFLYIPLFLVLYTLTEQFLHKIRNLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNR 90
+L +FL I + L+L + NLPP+P +PVIGHLHLLK+P++RT IS
Sbjct: 8 LLSFLFLTISIKLLLTKSNRK-----PNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKS 67
Query: 91 YG--PVVFLRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSY 150
G P+ LR G+R V V+SS S AEEC TKND+V ANRP ++++K GYN TN++ +SY
Sbjct: 68 LGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASY 127
Query: 151 GDHWRNLRRISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLT 210
GDHWRNLRRI+ VE+ S+HR+ S++R +E+ LI L+R +K+ +L
Sbjct: 128 GDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLA 187
Query: 211 YNIMLRMIVGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVE 270
+N ++ M+ GKR+YG +D DEA+ REL AE +G N+ D++P ++W+ + E
Sbjct: 188 FNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFE 247
Query: 271 NEIMECHKRRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDE 330
N+ R D +Q L+D++R + E G +T+I+ LL QE++PE Y D
Sbjct: 248 NQTKILGNRLDRVLQKLVDEKR---AEKEKG--------QTLIDHLLSFQETEPEYYTDV 307
Query: 331 TIKALMLVLLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANL 390
IK ++L L+ AGT+TS T+EWA+S +LN PE+L+KA+ EID ++G DRL++ESD+ NL
Sbjct: 308 IIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNL 367
Query: 391 PYLRGIINETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPR 450
YL+ I++ETLR+YP PL++PH SS +C V GY +PR T+L N+WA+H DP W EP
Sbjct: 368 HYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPE 427
Query: 451 KFDPERFERVESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELV 510
+F PERFE+ E LMPFG GRR CPG LG R++ L +G LIQ FEWER G ELV
Sbjct: 428 RFKPERFEK---EGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELV 487
Query: 511 DMTEGIAVSMPKAQRLLAKCTPRPI 531
DMTEG ++MPKA L A C R I
Sbjct: 488 DMTEGEGITMPKATPLRAMCKARAI 490
BLAST of Spg016936 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 459.1 bits (1180), Expect = 4.8e-129
Identity = 246/502 (49.00%), Postives = 324/502 (64.54%), Query Frame = 0
Query: 42 FLVLYTLTEQFLHKIR---NLPPTPFPSVPVIGHLHLLKKPLYRTLAKISNRYG--PVVF 101
FL L+TL L + + NLPP+P S+PVIGHLHLLK PL+RT +S G PV
Sbjct: 11 FLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFH 70
Query: 102 LRFGSRQVLVVSSPSAAEECLTKNDIVFANRPRLLLSKWFGYNNTNLVWSSYGDHWRNLR 161
LR G+R V V+SS S AEEC TKND+V ANRP+ +SK GYN T L+ +SYGDHWRNLR
Sbjct: 71 LRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLR 130
Query: 162 RISTVEVLSTHRLQMLSTVRLEEVNSLIRRLARSETQVH---NMKNAFFDLTYNIMLRMI 221
RI+ VE+ STHRL +R +E+ LI L+R MK +L N +RM+
Sbjct: 131 RIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIRML 190
Query: 222 VGKRFYGDDVDDADEARCYRELQAESTRLSGKSNIGDFIPLMSWLGFGSAVENEIMECHK 281
GKR++G+D DD A+ + L +E+ +G N D++ ++ W+ S+ E I
Sbjct: 191 AGKRYFGEDNDD---AKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYEKRIKNLGN 250
Query: 282 RRDAFMQSLIDQQRKRMVQTETGDSFRDGRKKTMIEVLLELQESQPEQYNDETIKALMLV 341
R D F+Q L+D++R + E G+ TMI+ LL LQ+ QP+ Y D IK ++L
Sbjct: 251 RFDTFLQKLVDEKR---AEKEKGE--------TMIDHLLALQDIQPDYYTDVIIKGIILT 310
Query: 342 LLAAGTETSVNTMEWALSLMLNQPEVLKKAQIEIDCQVGQDRLIDESDVANLPYLRGIIN 401
L+ AGT+TS T+EWA+S +LN PE+LKKA++EID +VG DRL+DESD+ NL YL+ I+
Sbjct: 311 LIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVL 370
Query: 402 ETLRMYPPGPLMVPHESSKDCSVGGYHIPRGTMLYVNLWAIHNDPKNWGEPRKFDPERFE 461
ETLRMYP PL++PH SS+DC VGGY IP GTM+ N WA+H DP+ W +P F PERFE
Sbjct: 371 ETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFE 430
Query: 462 RV-ESEKYGFNLMPFGAGRRGCPGEGLGLRMIGLVMGSLIQCFEWERPGEELVDMTEGIA 521
+ E+EK L+ FG GRR CPG GL R+I +GSL+QCFEWER GE+ VDMTE
Sbjct: 431 KEGEAEK----LISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKG 490
Query: 522 VSMPKAQRLLAKCTPRPIAEHL 535
++PKA L A C R I + L
Sbjct: 491 ATLPKAIPLRAMCKARSIVDKL 491
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| W8JMU7 | 1.4e-133 | 49.80 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
| Q6WNQ9 | 1.2e-127 | 47.24 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
| Q6WNQ8 | 1.3e-126 | 47.60 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
| Q9FG65 | 1.7e-126 | 48.12 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
| O65790 | 1.2e-124 | 45.54 | Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1FJC9 | 1.8e-248 | 80.54 | cytochrome P450 81D1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114459... | [more] |
| A0A6J1J228 | 6.8e-248 | 80.35 | cytochrome P450 81D1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481119... | [more] |
| A0A6J1CYI5 | 9.5e-242 | 78.79 | cytochrome P450 81D1-like OS=Momordica charantia OX=3673 GN=LOC111015737 PE=3 SV... | [more] |
| A0A1S3BUR6 | 4.1e-237 | 77.13 | cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103493892 PE=3 SV=1 | [more] |
| A0A0A0L2I7 | 3.9e-235 | 75.78 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642300 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G37360.1 | 7.2e-133 | 48.81 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
| AT4G37340.1 | 8.0e-132 | 49.50 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
| AT4G37370.1 | 3.3e-130 | 50.10 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT4G37320.1 | 4.8e-129 | 46.93 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
| AT4G37330.1 | 4.8e-129 | 49.00 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
