Spg016891 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016891
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold9: 43520494 .. 43524333 (+)
RNA-Seq ExpressionSpg016891
SyntenySpg016891
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTAATTGTGATGTGTAGTCGGGTATGTAGGTAAGTATCGTCCTTTTACAAGTGTACTAAAAATATTATCCAAACAAAACCTAAACTCAAACTTTCACGGCGGACTTTCTTTCTTCAAAATAAAAATAAATAAATTAAAAATTAAAAAAAATTAAAAAAAAAAAATGAAGGAGAAAACCTAACTGTCAGTGTCATTGTGGTGGTGTTCAGTTACACCGAACAAGTGGACAAATCCGTAATTTCCATAAATAATGCCATTGCCGCTGCCGGCCCAGCCCGCTTCTCTTCCTCTATTTCTCTCTCCACCGCTCTCCATTTTTACACAAATTATTCAACTTTTGATTCTTTTCAATTCCCTTTCCCCATTTCTTCTCTTCTCTTACACTCACTCACTCCCATGTTTCTTCTTTAGCTCCCGCAGATTTCTCCTTCCAAGTTCCAGTTTCCCACTTCGGTATCTTCTTCTCTTCTCTGTAAACCCTTTATTTTATGTTTAAGTTCTTCCTGTTTTCTCTCTGCTCTTGGTTCGGTTCCCACGAAACCGACAGAAACAGGGTCTATCTTATTTCTTTGGAATTGGGTCTTCTTGTGATTTGAAGTTCATTGGATTTTGTGCAGTTATGTTTTCTTTTGCCGACTTAATCGGCCTGCTGGTTGTGTTTTTGGTGTGATTCTTTGTTTGTTCCAATTGGGTTCTGATTTTCATGCTAGTTGTTGATTTTTTATTCAATACTTTATTCTAGTTTTGTGTTTTATTTTCAATCTGATGCATTTTTGTTTTGTGGGTGTGGGGTTTTGTGTTTGTTTTCCTCTTGTTTCAGATTTGAGGAATGGCTGCACAAGAAGCTGGTTCTCAACCGGTTCCTTCTTTTGAGTATGAGCTTCTTGATGGTGATCCTGATCATGTCAGGACAGTGGTTGCTTCATCAAACTATCCAAGCCCTAGGATTGAACCTTCAAAGGTCAAGCTTAGGCATCGAATTGGGCGGGGCGTGTTCGGCGACGTTTGGTTAGCAACTCACCATCAGTCCACTAAGGATTATGATGAGTACCATGAAGTTGCTGTTAAGATGTTGAATCCTGTTAAAGAAGACCATATGAGGGTTGTTTTGGATAAACTTGAGGATCATTTCTATAAGTGTCAAGGTGCAAAGAGTGTTTGCAGGCTGTATGGAGTGTCAATAATTAGTGGGAAGGTAATTCTGGTATCTGTAGCTGCTGCATTTTAGGGCCATTTTGTTGGAAATATATATGTATGTTGCTGAGATGTTACTGTCCTTTGTGTGTTCTTCCAGTTATGCATCATCATGAATTTCTATGAGGGATCAGTTGCTGATAAAATGGCTCGCCTGAAAGAAGGAAAGCTTTCACTCCCTGATGTATTGAGGTGATATACTGATATGGGTTTGAATTTTTAGGTGGAAAGAAAAGTTATTAAATCTCTGTTGTATCTTTTAGATTAGTTCTTTCAGGGTTTTGAACTTATAACAAACTCTTTTGGGGCAAAATTCTCCACCTATGCTGTTTGTTGCAGATATGGAATCAATCTAGCCCAAGGGATTTTGGAATTGCACTCAATGGAGATCTTGGTTCTTAACCTGAAACCTTCAAACATGCTCCTTACTACCAAGGACCAAGCAATTTTAGGAGATCTAGGAATTCCTTACCTATTGCACGGTGTACCGATACCAAATACGGATGTGATTCAGAGACTTGGGACACCTAACTATATGGCACCAGAACAATGGCAGCCAGAAGTTAGAGGTCCGATATCGTATGAAACTGATTCGTGGGGGTTTGCCTGCTGCATGGTTGAGATGTTGACTGGTGTTCAGCCGTGGCGGGGGAAATCGGTTGATGAAATATTTCATTCTGTTGTCAGGAAACAAGAAAAGCCATGTATTCCAAGTGGACTTCCTCCTCTAATTGAGAATGTTCTTCTTGGTTGCTTTGAATATGACTTGAGGAGTCGTCCTCTGATGACTGACATATTACATGTGTTCCAAAGGTATTCATCTCATTCATGTCTGCTCTATTCTCACATGAAATATTGACTTCCCTAATCCAAATCCAAAAGTGCTTCCTTCTTCAGATTGTAAGAGGTTATGGCTTTCTTTCTATTCCTTTGTTTTGTTTCTTTCTTTGTGGATCTATGACTTGGTTGAGGTGTATAATTTGATCCCTTTTGGTTTCTTTTGAATTTCCTACTTGAATCATTTATGAATTAAACCAGCACTTTTAACTGGTCTTTTTCTTTCTGTACTATCCAACTAAATTTTTAGACTTCAAATTTTGGTCACTAACAGAATTTGTAAACAGTAAGGGACATGAGAAGTGTGTTTTAAAGAGAGGTTTTTATGTAAGGAATGGCTACAATCTACCTTTGAAGCCAAGAACCAATATCCCAGTTTGCACCCTTCTCTCTTCTTGTTGCCTTGTTAAAATTTGTGCAGCACCTGCATTAACAAAAGCATATAACTGAACCACCTTGATTATGAATAGGATATTAGGAACAGCAGAATTTTAGAAGTGTACCTTATAGATATGCAGGGCTGAGAAATGGCACTGTTCAGTTTTTTAATGGCTGCTCTTTTTTTACCATCTCTTCTAACTTGTTTAGTTGATGGAGTTAATTCTGCTTTGTAGCTCTCAACAAGTTGTTAATAGCGACTGGCAAGCCATTGGGAGTTCAAAAGTGTTGAACAAATCAAGTGCCACTGGACATACTGAATGGTTTCTCTCAAAGGATCACTTGCAAGTAAATGACTTGGTGCGTTCTAGAAAGCCATTAAACTCTTGCAAATCAGACAATATGAACATCCCTGAAGGAAGGATAGTGGGTTTGGAAGGCGAAACTGGAAAGGATGCTTTTGTCTTGGTGAGGGTTCGTGGAATCCACGATCCTGTAAGAGTGTATGCTTCGACGCTTGAGCGGGTGAGTTTCGGGTTAGCAGCTGGTGACTGGATACGTCTGAAGGAAGCAGAAAAGAAACACTCTCCAGTGGGAATCCTTCATTCAATCGACCGTGTTGGTAATGTTGCTGTTGCTTTTATTGGTGTAGAAACTCTCTGGAAGGGGAACTCTTCCCAGTTTCAGATGGCAGAGTCGTTTTGCGTTGGCCAATTTGTACGGATAAAAGCCAGCATTCTGAGACCCCGATTCGAATGGCCTCGTAAAAAAGGTGTTTGGGCAACAGGGAGGATTTGGTGGATCCTTCCCAATGGCTGCCTCATGGTCAAATTCCCTGGAATTCTCTCTTTCAAAGAAGAATGTGATACATATATGGCCGATCCAGCCGAGGTCGAGGCGGTTAATTTTAGCACTTGTCCTGGGATGGTGAAGAAGTACCAACATCTTGAGGATTTTCACTGGTCAGTAAGACCAGTTTTGATTGCATTTGGCCTGTTTACAGCAATGAAGCTGGGATTTGCATTTGGGAAGAAAGTGGGAAGATCAAAGGTGAAGAAGGGGCAAGGCAACCTTGTGTACTGTGAAACTCAACATGTTGAAGGTCAGAATGCCAACAATCCAGCTTGGATTCCTCCACCTGTGAAGAACATTCTTTTCGGAGATGGTGTTAGCACGGTCGCTCGATGATTCCACGGTCAGTTTGTTGTTCCATCAGCTCAAAGTTGTGCCAGTTATCTGTATAGATTAACTTTACTGACTGGTTTATACTCCCAGTACTATGTAAATGCTCCTGTACAGTTGTGCAACAATCAAACAAACAAACCGTAAGAATGTGACTGTGTATGTAAAGAAAAATAATATGTTGTTTGTATAAAAAAAGTTATATGTTTGATCTTTCATGCTTTCTGGTGGATAGATCAGTCCTTG

mRNA sequence

ATGGCTGCACAAGAAGCTGGTTCTCAACCGGTTCCTTCTTTTGAGTATGAGCTTCTTGATGGTGATCCTGATCATGTCAGGACAGTGGTTGCTTCATCAAACTATCCAAGCCCTAGGATTGAACCTTCAAAGGTCAAGCTTAGGCATCGAATTGGGCGGGGCGTGTTCGGCGACGTTTGGTTAGCAACTCACCATCAGTCCACTAAGGATTATGATGAGTACCATGAAGTTGCTGTTAAGATGTTGAATCCTGTTAAAGAAGACCATATGAGGGTTGTTTTGGATAAACTTGAGGATCATTTCTATAAGTGTCAAGGTGCAAAGAGTGTTTGCAGGCTGTATGGAGTGTCAATAATTAGTGGGAAGTTATGCATCATCATGAATTTCTATGAGGGATCAGTTGCTGATAAAATGGCTCGCCTGAAAGAAGGAAAGCTTTCACTCCCTGATGTATTGAGATATGGAATCAATCTAGCCCAAGGGATTTTGGAATTGCACTCAATGGAGATCTTGGTTCTTAACCTGAAACCTTCAAACATGCTCCTTACTACCAAGGACCAAGCAATTTTAGGAGATCTAGGAATTCCTTACCTATTGCACGGTGTACCGATACCAAATACGGATGTGATTCAGAGACTTGGGACACCTAACTATATGGCACCAGAACAATGGCAGCCAGAAGTTAGAGGTCCGATATCGTATGAAACTGATTCGTGGGGGTTTGCCTGCTGCATGGTTGAGATGTTGACTGGTGTTCAGCCGTGGCGGGGGAAATCGGTTGATGAAATATTTCATTCTGTTGTCAGGAAACAAGAAAAGCCATGTATTCCAAGTGGACTTCCTCCTCTAATTGAGAATGTTCTTCTTGGTTGCTTTGAATATGACTTGAGGAGTCGTCCTCTGATGACTGACATATTACATGTGTTCCAAAGCTCTCAACAAGTTGTTAATAGCGACTGGCAAGCCATTGGGAGTTCAAAAGTGTTGAACAAATCAAGTGCCACTGGACATACTGAATGGTTTCTCTCAAAGGATCACTTGCAAGTAAATGACTTGGTGCGTTCTAGAAAGCCATTAAACTCTTGCAAATCAGACAATATGAACATCCCTGAAGGAAGGATAGTGGGTTTGGAAGGCGAAACTGGAAAGGATGCTTTTGTCTTGGTGAGGGTTCGTGGAATCCACGATCCTGTAAGAGTGTATGCTTCGACGCTTGAGCGGGTGAGTTTCGGGTTAGCAGCTGGTGACTGGATACGTCTGAAGGAAGCAGAAAAGAAACACTCTCCAGTGGGAATCCTTCATTCAATCGACCGTGTTGGTAATGTTGCTGTTGCTTTTATTGGTGTAGAAACTCTCTGGAAGGGGAACTCTTCCCAGTTTCAGATGGCAGAGTCGTTTTGCGTTGGCCAATTTGTACGGATAAAAGCCAGCATTCTGAGACCCCGATTCGAATGGCCTCGTAAAAAAGGTGTTTGGGCAACAGGGAGGATTTGGTGGATCCTTCCCAATGGCTGCCTCATGGTCAAATTCCCTGGAATTCTCTCTTTCAAAGAAGAATGTGATACATATATGGCCGATCCAGCCGAGGTCGAGGCGGTTAATTTTAGCACTTGTCCTGGGATGGTGAAGAAGTACCAACATCTTGAGGATTTTCACTGGTCAGTAAGACCAGTTTTGATTGCATTTGGCCTGTTTACAGCAATGAAGCTGGGATTTGCATTTGGGAAGAAAGTGGGAAGATCAAAGGTGAAGAAGGGGCAAGGCAACCTTGTGTACTGTGAAACTCAACATGTTGAAGGTCAGAATGCCAACAATCCAGCTTGGATTCCTCCACCTGTGAAGAACATTCTTTTCGGAGATGGTGTTAGCACGGTCGCTCGATGA

Coding sequence (CDS)

ATGGCTGCACAAGAAGCTGGTTCTCAACCGGTTCCTTCTTTTGAGTATGAGCTTCTTGATGGTGATCCTGATCATGTCAGGACAGTGGTTGCTTCATCAAACTATCCAAGCCCTAGGATTGAACCTTCAAAGGTCAAGCTTAGGCATCGAATTGGGCGGGGCGTGTTCGGCGACGTTTGGTTAGCAACTCACCATCAGTCCACTAAGGATTATGATGAGTACCATGAAGTTGCTGTTAAGATGTTGAATCCTGTTAAAGAAGACCATATGAGGGTTGTTTTGGATAAACTTGAGGATCATTTCTATAAGTGTCAAGGTGCAAAGAGTGTTTGCAGGCTGTATGGAGTGTCAATAATTAGTGGGAAGTTATGCATCATCATGAATTTCTATGAGGGATCAGTTGCTGATAAAATGGCTCGCCTGAAAGAAGGAAAGCTTTCACTCCCTGATGTATTGAGATATGGAATCAATCTAGCCCAAGGGATTTTGGAATTGCACTCAATGGAGATCTTGGTTCTTAACCTGAAACCTTCAAACATGCTCCTTACTACCAAGGACCAAGCAATTTTAGGAGATCTAGGAATTCCTTACCTATTGCACGGTGTACCGATACCAAATACGGATGTGATTCAGAGACTTGGGACACCTAACTATATGGCACCAGAACAATGGCAGCCAGAAGTTAGAGGTCCGATATCGTATGAAACTGATTCGTGGGGGTTTGCCTGCTGCATGGTTGAGATGTTGACTGGTGTTCAGCCGTGGCGGGGGAAATCGGTTGATGAAATATTTCATTCTGTTGTCAGGAAACAAGAAAAGCCATGTATTCCAAGTGGACTTCCTCCTCTAATTGAGAATGTTCTTCTTGGTTGCTTTGAATATGACTTGAGGAGTCGTCCTCTGATGACTGACATATTACATGTGTTCCAAAGCTCTCAACAAGTTGTTAATAGCGACTGGCAAGCCATTGGGAGTTCAAAAGTGTTGAACAAATCAAGTGCCACTGGACATACTGAATGGTTTCTCTCAAAGGATCACTTGCAAGTAAATGACTTGGTGCGTTCTAGAAAGCCATTAAACTCTTGCAAATCAGACAATATGAACATCCCTGAAGGAAGGATAGTGGGTTTGGAAGGCGAAACTGGAAAGGATGCTTTTGTCTTGGTGAGGGTTCGTGGAATCCACGATCCTGTAAGAGTGTATGCTTCGACGCTTGAGCGGGTGAGTTTCGGGTTAGCAGCTGGTGACTGGATACGTCTGAAGGAAGCAGAAAAGAAACACTCTCCAGTGGGAATCCTTCATTCAATCGACCGTGTTGGTAATGTTGCTGTTGCTTTTATTGGTGTAGAAACTCTCTGGAAGGGGAACTCTTCCCAGTTTCAGATGGCAGAGTCGTTTTGCGTTGGCCAATTTGTACGGATAAAAGCCAGCATTCTGAGACCCCGATTCGAATGGCCTCGTAAAAAAGGTGTTTGGGCAACAGGGAGGATTTGGTGGATCCTTCCCAATGGCTGCCTCATGGTCAAATTCCCTGGAATTCTCTCTTTCAAAGAAGAATGTGATACATATATGGCCGATCCAGCCGAGGTCGAGGCGGTTAATTTTAGCACTTGTCCTGGGATGGTGAAGAAGTACCAACATCTTGAGGATTTTCACTGGTCAGTAAGACCAGTTTTGATTGCATTTGGCCTGTTTACAGCAATGAAGCTGGGATTTGCATTTGGGAAGAAAGTGGGAAGATCAAAGGTGAAGAAGGGGCAAGGCAACCTTGTGTACTGTGAAACTCAACATGTTGAAGGTCAGAATGCCAACAATCCAGCTTGGATTCCTCCACCTGTGAAGAACATTCTTTTCGGAGATGGTGTTAGCACGGTCGCTCGATGA

Protein sequence

MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVWLATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWGFACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRPLMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLNSCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRLKEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILRPRFEWPRKKGVWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCPGMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVEGQNANNPAWIPPPVKNILFGDGVSTVAR
Homology
BLAST of Spg016891 vs. NCBI nr
Match: XP_038898209.1 (probable serine/threonine-protein kinase DDB_G0292354 [Benincasa hispida])

HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 594/628 (94.59%), Postives = 612/628 (97.45%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVK+DH+RVVLDKLEDHFYKCQGAK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKDDHLRVVLDKLEDHFYKCQGAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGINLAQGILELHS EILVLNLKPSNM
Sbjct: 121 GKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGINLAQGILELHSKEILVLNLKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLHGVPIPN D+IQRLGTPNYMAPEQWQPEVRGP+SYETDSWG
Sbjct: 181 LLTTKDQAILGDVGIPFLLHGVPIPNMDIIQRLGTPNYMAPEQWQPEVRGPVSYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           L+TDIL+VFQSSQ  VNSDWQAIGSSKVLNKS+ATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LLTDILNVFQSSQH-VNSDWQAIGSSKVLNKSNATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG+IVGLE ET KDAFVLVRVRGIHDPVRVYASTLERVSFGLA GDWIRL
Sbjct: 361 SCKSDNMNIPEGKIVGLERETEKDAFVLVRVRGIHDPVRVYASTLERVSFGLAVGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLW GNSSQFQMAESFCVGQFVRIKASILR
Sbjct: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWNGNSSQFQMAESFCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKKG VWATGRIWWILPNGCLMVKFPGILSFKEECD+YMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGNVWATGRIWWILPNGCLMVKFPGILSFKEECDSYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRPVLIAFG+FTAMKLG AFGKKVGRSKVKKGQ NLVYCE+QH+E
Sbjct: 541 GMVKKYQHLEDFHWSVRPVLIAFGMFTAMKLGIAFGKKVGRSKVKKGQSNLVYCESQHIE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQNANNPAWIPPPVKNILFGDGVSTVAR
Sbjct: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 627

BLAST of Spg016891 vs. NCBI nr
Match: KAA0038817.1 (E3 ubiquitin-protein ligase KEG [Cucumis melo var. makuwa])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 586/628 (93.31%), Postives = 605/628 (96.34%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGDPDHVRTVVASSNY SPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDPDHVRTVVASSNYSSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQ AK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQAAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGINLAQGILELHS EILVLNLKPSNM
Sbjct: 121 GKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGINLAQGILELHSKEILVLNLKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLHGVPIPN+D+IQRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLTTKDQAILGDVGIPFLLHGVPIPNSDIIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VF SSQ  VN DWQ IGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFLSSQH-VNGDWQDIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG++VGLEGET KDAFVLVRVRGIHDPVRVYAS LERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGKVVGLEGETEKDAFVLVRVRGIHDPVRVYASMLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEA+KKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAES CVGQFVRIKASILR
Sbjct: 421 KEADKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESLCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKKG VWATG+IWWILPNGCLMVKFPGILSFKEEC++YMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGNVWATGKIWWILPNGCLMVKFPGILSFKEECNSYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRP+LIAFG+FTAMKLG AFG KVGRSKVKKGQ NLVYCE+QH E
Sbjct: 541 GMVKKYQHLEDFHWSVRPILIAFGMFTAMKLGIAFG-KVGRSKVKKGQSNLVYCESQHTE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQN NNPAWIPPPVKNILFGDGV+TV R
Sbjct: 601 GQNTNNPAWIPPPVKNILFGDGVNTVTR 626

BLAST of Spg016891 vs. NCBI nr
Match: XP_008466423.1 (PREDICTED: E3 ubiquitin-protein ligase KEG [Cucumis melo] >XP_008466424.1 PREDICTED: E3 ubiquitin-protein ligase KEG [Cucumis melo] >TYK31440.1 E3 ubiquitin-protein ligase KEG [Cucumis melo var. makuwa])

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 586/628 (93.31%), Postives = 605/628 (96.34%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGDPDHVRTVVASSNY SPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDPDHVRTVVASSNYSSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQ AK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQAAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGINLAQGILELHS EILVLNLKPSNM
Sbjct: 121 GKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGINLAQGILELHSKEILVLNLKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLHGVPIPN+D+IQRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLTTKDQAILGDVGIPFLLHGVPIPNSDIIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VF SSQ  VN DWQ IGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFLSSQH-VNGDWQDIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG++VGLEGET KDAFVLVRVRGIHDPVRVYAS LERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGKVVGLEGETEKDAFVLVRVRGIHDPVRVYASMLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEA+KKHS VGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR
Sbjct: 421 KEADKKHSSVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKKG VWATG+IWWILPNGCLMVKFPGILSFKEEC++YMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGNVWATGKIWWILPNGCLMVKFPGILSFKEECNSYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRP+LIAFG+FTAMKLG AFG KVGRSKVKKGQ NLVYCE+QH E
Sbjct: 541 GMVKKYQHLEDFHWSVRPILIAFGMFTAMKLGIAFG-KVGRSKVKKGQSNLVYCESQHTE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQN NNPAWIPPPVKNILFGDGV+TV R
Sbjct: 601 GQNTNNPAWIPPPVKNILFGDGVNTVTR 626

BLAST of Spg016891 vs. NCBI nr
Match: XP_004136355.1 (E3 ubiquitin-protein ligase KEG [Cucumis sativus] >XP_011652477.1 E3 ubiquitin-protein ligase KEG [Cucumis sativus] >KGN60091.1 hypothetical protein Csa_000762 [Cucumis sativus])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 581/628 (92.52%), Postives = 602/628 (95.86%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGD DHVRTVVASSNY SPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDTDHVRTVVASSNYSSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLED FYKCQ AK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDRFYKCQAAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGS+ADKMARLK+GKLS PDVLRYGINLAQGI ELHS EILVLN+KPSNM
Sbjct: 121 GKLCIIMKFYEGSIADKMARLKDGKLSPPDVLRYGINLAQGIFELHSKEILVLNMKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLH VPIPN+D++QRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLTTKDQAILGDIGIPFLLHSVPIPNSDIVQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VFQS Q  VN DWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFQSFQH-VNGDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG+IVGLEGET KDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGKIVGLEGETEKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEA+KKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR
Sbjct: 421 KEADKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEW RKKG VWATG+IWWILPNGCLMVKFPGILSFKEEC++YMADPAEVE VNFSTCP
Sbjct: 481 PRFEWLRKKGSVWATGKIWWILPNGCLMVKFPGILSFKEECNSYMADPAEVEVVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRP+LIAFG+FTAMKLG AFG KVGRSKVKKGQ NLVYCE+QHVE
Sbjct: 541 GMVKKYQHLEDFHWSVRPILIAFGMFTAMKLGIAFG-KVGRSKVKKGQSNLVYCESQHVE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQN NNPAWIPPPVKNILFGD V+TVAR
Sbjct: 601 GQNTNNPAWIPPPVKNILFGDSVNTVAR 626

BLAST of Spg016891 vs. NCBI nr
Match: XP_023535362.1 (E3 ubiquitin-protein ligase KEG [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 583/629 (92.69%), Postives = 600/629 (95.39%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           MA QEA SQ VPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MATQEAASQAVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LAT HQSTKDYDEYHEVAVKMLNPVKEDHMRVV DKLEDHFYKCQG KSVCRLYGVSII 
Sbjct: 61  LATLHQSTKDYDEYHEVAVKMLNPVKEDHMRVVFDKLEDHFYKCQGGKSVCRLYGVSIID 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
            KLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGI+L+QGILELHS E+LVLNLKPSN+
Sbjct: 121 EKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGISLSQGILELHSKEVLVLNLKPSNV 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LL+TKDQAILGDLGIP+LL GVPIPNTD+IQRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLSTKDQAILGDLGIPFLLRGVPIPNTDIIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           F CC+VEMLTGV PW GKSVDEIFHSVVRKQEKPCIPSGLPPLIEN L GCFEYDLRSRP
Sbjct: 241 FGCCVVEMLTGVPPWHGKSVDEIFHSVVRKQEKPCIPSGLPPLIENALFGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VFQSS Q VN+DWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFQSSLQGVNNDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEGRIVGLE ET KDAFVLVRVRGIHDPVRV ASTLERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGRIVGLERETEKDAFVLVRVRGIHDPVRVCASTLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KE+EKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKAS+LR
Sbjct: 421 KESEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASVLR 480

Query: 481 PRFEWPRKK-GVWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKK GVWATGRIWW+LPNGCLMVKFPGIL+FKEECDTYMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGGVWATGRIWWVLPNGCLMVKFPGILAFKEECDTYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQH-V 600
           GMVKKYQHLEDFHWSVRPVL+AFGLFTAMKLG AFGKKVGRSKVKK QGNLVYCE+QH  
Sbjct: 541 GMVKKYQHLEDFHWSVRPVLMAFGLFTAMKLGIAFGKKVGRSKVKKPQGNLVYCESQHTA 600

Query: 601 EGQNANNPAWIPPPVKNILFGDGVSTVAR 628
           EGQNANNPAWIPPPVKNILF DG  TVAR
Sbjct: 601 EGQNANNPAWIPPPVKNILFRDGAGTVAR 629

BLAST of Spg016891 vs. ExPASy Swiss-Prot
Match: Q9FY48 (E3 ubiquitin-protein ligase KEG OS=Arabidopsis thaliana OX=3702 GN=KEG PE=1 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 1.6e-32
Identity = 82/259 (31.66%), Postives = 133/259 (51.35%), Query Frame = 0

Query: 75  HEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSV 134
           H VAVK +   ++  +  +  +LE         ++VC  +GV  + G LC++M+   GSV
Sbjct: 171 HRVAVKKMTLTEDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSV 230

Query: 135 ADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLG 194
             +M R  EG+L+L  +LRYG ++A+G+ ELH+  ++ +N+KPSN+LL     A++ D G
Sbjct: 231 QSEMQR-NEGRLTLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYG 290

Query: 195 IPYLLHGVPIPN-------------TDVIQRLGTPNYMAPEQWQP------EVRGPISYE 254
           +  +L                    TD +    +P+Y APE W P      E    +S E
Sbjct: 291 LAPILKKPTCQKTRPEFDSSKVTLYTDCVTL--SPHYTAPEAWGPVKKLFWEDASGVSPE 350

Query: 255 TDSWGFACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKP-----CIPSGLPPLIENVLLG 310
           +D+W F C +VEM TG  PW G S +EIF +VV+ ++ P      +  G+P  +  ++  
Sbjct: 351 SDAWSFGCTLVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGE 410

BLAST of Spg016891 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 2.3e-18
Identity = 71/279 (25.45%), Postives = 132/279 (47.31%), Query Frame = 0

Query: 40  IEPSKVKLRHRIGRGVFGDVWLATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLED 99
           I+ +++K+  ++  G +GD+   T+           EVA+K L P + ++  +     E 
Sbjct: 281 IDVTQLKIEKKVASGSYGDLHRGTYCS--------QEVAIKFLKPDRVNNEMLREFSQEV 340

Query: 100 HFYKCQGAKSVCRLYGVSIISGKLCIIMNFY-EGSVADKMARLKEGKLSLPDVLRYGINL 159
              +    K+V +  G    S  LCI+  F   GS+ D + + K     L  +L+  +++
Sbjct: 341 FIMRKVRHKNVVQFLGACTRSPTLCIVTEFMARGSIYDFLHKQK-CAFKLQTLLKVALDV 400

Query: 160 AQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNY 219
           A+G+  LH   I+  +LK +N+L+       + D G+      V I +  +    GT  +
Sbjct: 401 AKGMSYLHQNNIIHRDLKTANLLMDEHGLVKVADFGVA----RVQIESGVMTAETGTYRW 460

Query: 220 MAPEQWQPEVRGPISYETDSWGFACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPS 279
           MAPE  + +   P +++ D + +A  + E+LTG  P+   +  +    VV+K  +P IP 
Sbjct: 461 MAPEVIEHK---PYNHKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPK 520

Query: 280 GLPPLIENVLLGCFEYDLRSRPLMTDILHVFQSSQQVVN 318
              P ++ +L  C+  D   RPL  +I+ + Q   + VN
Sbjct: 521 KTHPKVKGLLERCWHQDPEQRPLFEEIIEMLQQIMKEVN 543

BLAST of Spg016891 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.6e-18
Identity = 71/292 (24.32%), Postives = 127/292 (43.49%), Query Frame = 0

Query: 45  VKLRHRIGRGVFGDVWLATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKC 104
           +K  H+I  G +GD++  T+           EVA+K+L P + D         E    + 
Sbjct: 290 LKFGHKIASGSYGDLYKGTYCS--------QEVAIKVLKPERLDSDLEKEFAQEVFIMRK 349

Query: 105 QGAKSVCRLYGVSIISGKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILE 164
              K+V +  G       LCI+  F  G         ++G   LP + +  I++ +G+  
Sbjct: 350 VRHKNVVQFIGACTKPPHLCIVTEFMPGGSVYDYLHKQKGVFKLPTLFKVAIDICKGMSY 409

Query: 165 LHSMEILVLNLKPSNMLLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQW 224
           LH   I+  +LK +N+L+   +   + D G+  +     +   +     GT  +MAPE  
Sbjct: 410 LHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTGVMTAET----GTYRWMAPEVI 469

Query: 225 QPEVRGPISYETDSWGFACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLI 284
           + +   P  ++ D + +   + E+LTG  P+   +  +    VV+K  +P IP    P +
Sbjct: 470 EHK---PYDHKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKL 529

Query: 285 ENVLLGCFEYDLRSRPLMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATG 337
             +L   +E+D   RP  ++I+   Q   + V  +    G  K   K S+TG
Sbjct: 530 AELLERLWEHDSTQRPDFSEIIEQLQEIAKEVGEE----GEEK---KKSSTG 559

BLAST of Spg016891 vs. ExPASy Swiss-Prot
Match: Q54RZ7 (Probable serine/threonine-protein kinase DDB_G0282895 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0282895 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 4.7e-16
Identity = 72/277 (25.99%), Postives = 136/277 (49.10%), Query Frame = 0

Query: 39   RIEPSKVKLRHRIGRGVFGDVWLATHHQSTKDYDEYHEVAVKMLNPVK-----EDHMR-V 98
            +IE S++++   +G G   +V         K   +  EVAVK+ N        ED ++ V
Sbjct: 1371 QIEESELQILQFLGEGALAEV--------HKGIWKGKEVAVKIFNEGSFSFRLEDFLKEV 1430

Query: 99   VLDKLEDH--FYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSVADKMARLKEGKLSLPD 158
             +  L  H    K +GA    R    S  S  + +    ++G++ + + + K   LSL D
Sbjct: 1431 AILGLISHPNLLKLKGACIAPR----SHKSTFMIVTELMHKGTLLEVINKNK--PLSLED 1490

Query: 159  VLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLGIPYLLHGVPIPNTDVI 218
            +++Y +++AQG+  LHS++ +  ++K +N+L+   + A +GD G+  ++      N ++ 
Sbjct: 1491 IIKYALSVAQGLAYLHSVDFIHRDIKAANILVDKNNNAKVGDFGLSRVIDN----NFNMT 1550

Query: 219  QRLGTPNYMAPEQWQPEVRGPISYETDSWGFACCMVEMLTGVQPW-RGKSVDEIFHSVVR 278
               GTP + +PE    E     +  +D + +   + E+ TG +P+   +S+ E+  SV  
Sbjct: 1551 AVAGTPKWESPECLMGEA---YTSASDVYSYGMMLFELATGDEPFLEIQSIVELARSVCD 1610

Query: 279  KQEKPCIPSGLPPLIENVLLGCFEYDLRSRPLMTDIL 307
            K+ KP I S +P  I +++  C     + RP M  I+
Sbjct: 1611 KKLKPKISSSVPNFISSLIKDCLHNSPKKRPTMNQII 1626

BLAST of Spg016891 vs. ExPASy Swiss-Prot
Match: G5EFD2 (Kinase suppressor of Ras A OS=Caenorhabditis elegans OX=6239 GN=ksr-1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.2e-13
Identity = 69/272 (25.37%), Postives = 123/272 (45.22%), Query Frame = 0

Query: 51  IGRGVFGDVWLATHHQSTKDYDEYHEVAVKM--LNPVKEDHMRVVLDKLEDHFYKCQGAK 110
           IGRG FG V    H         Y +VAVK+  +  + +   +    KLE   YK     
Sbjct: 483 IGRGRFGKVLRGFH---------YGDVAVKVYTMEHISDASKKAEEFKLEVSAYKNTRHD 542

Query: 111 SVCRLYGVSIISGKLCIIMNFYEGSVA-DKMARLKEGKLSLPDVLRYGINLAQGILELHS 170
           ++    G  +  G+  ++M+  +GS +   +  +   KL L    +    + Q +  LH+
Sbjct: 543 NIALFLGYFMSDGQYGMVMSLSKGSQSLYTLLHVVREKLDLATTRKIAQQICQAVSYLHT 602

Query: 171 MEILVLNLKPSNMLLTTKDQAILGDLGI---PYLLHGVPIPNTDVIQRLGTPNYMAPE-- 230
            +IL  +L+  N+LL +K++ ++ D GI     L H  P   +  +      NY+APE  
Sbjct: 603 KKILHKDLRSKNILLESKNKVVITDFGILSMKRLAH--PKQKSGYLTSKFWTNYIAPELA 662

Query: 231 --------QWQPEVRGPISYETDSWGFACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKP 290
                   +++ +   P S  +D + F C   EMLTG  P+ G+   +I  +   +  +P
Sbjct: 663 MAMRTEYDEYECD-DFPFSENSDVYAFGCVWFEMLTGALPYAGELPHQILFAKT-QGIRP 722

Query: 291 CIPS-GLPPLIENVLLGCFEYDLRSRPLMTDI 306
            +P+      ++ +L+ C+    + RP +TDI
Sbjct: 723 VLPNVKCTQELKELLVSCWNTAPQDRPTLTDI 741

BLAST of Spg016891 vs. ExPASy TrEMBL
Match: A0A5A7TB54 (E3 ubiquitin-protein ligase KEG OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G003760 PE=4 SV=1)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 586/628 (93.31%), Postives = 605/628 (96.34%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGDPDHVRTVVASSNY SPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDPDHVRTVVASSNYSSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQ AK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQAAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGINLAQGILELHS EILVLNLKPSNM
Sbjct: 121 GKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGINLAQGILELHSKEILVLNLKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLHGVPIPN+D+IQRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLTTKDQAILGDVGIPFLLHGVPIPNSDIIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VF SSQ  VN DWQ IGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFLSSQH-VNGDWQDIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG++VGLEGET KDAFVLVRVRGIHDPVRVYAS LERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGKVVGLEGETEKDAFVLVRVRGIHDPVRVYASMLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEA+KKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAES CVGQFVRIKASILR
Sbjct: 421 KEADKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESLCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKKG VWATG+IWWILPNGCLMVKFPGILSFKEEC++YMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGNVWATGKIWWILPNGCLMVKFPGILSFKEECNSYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRP+LIAFG+FTAMKLG AFG KVGRSKVKKGQ NLVYCE+QH E
Sbjct: 541 GMVKKYQHLEDFHWSVRPILIAFGMFTAMKLGIAFG-KVGRSKVKKGQSNLVYCESQHTE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQN NNPAWIPPPVKNILFGDGV+TV R
Sbjct: 601 GQNTNNPAWIPPPVKNILFGDGVNTVTR 626

BLAST of Spg016891 vs. ExPASy TrEMBL
Match: A0A1S3CR79 (E3 ubiquitin-protein ligase KEG OS=Cucumis melo OX=3656 GN=LOC103503837 PE=4 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 586/628 (93.31%), Postives = 605/628 (96.34%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGDPDHVRTVVASSNY SPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDPDHVRTVVASSNYSSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQ AK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQAAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGINLAQGILELHS EILVLNLKPSNM
Sbjct: 121 GKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGINLAQGILELHSKEILVLNLKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLHGVPIPN+D+IQRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLTTKDQAILGDVGIPFLLHGVPIPNSDIIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VF SSQ  VN DWQ IGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFLSSQH-VNGDWQDIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG++VGLEGET KDAFVLVRVRGIHDPVRVYAS LERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGKVVGLEGETEKDAFVLVRVRGIHDPVRVYASMLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEA+KKHS VGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR
Sbjct: 421 KEADKKHSSVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKKG VWATG+IWWILPNGCLMVKFPGILSFKEEC++YMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGNVWATGKIWWILPNGCLMVKFPGILSFKEECNSYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRP+LIAFG+FTAMKLG AFG KVGRSKVKKGQ NLVYCE+QH E
Sbjct: 541 GMVKKYQHLEDFHWSVRPILIAFGMFTAMKLGIAFG-KVGRSKVKKGQSNLVYCESQHTE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQN NNPAWIPPPVKNILFGDGV+TV R
Sbjct: 601 GQNTNNPAWIPPPVKNILFGDGVNTVTR 626

BLAST of Spg016891 vs. ExPASy TrEMBL
Match: A0A5D3E732 (E3 ubiquitin-protein ligase KEG OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007530 PE=4 SV=1)

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 586/628 (93.31%), Postives = 605/628 (96.34%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGDPDHVRTVVASSNY SPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDPDHVRTVVASSNYSSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQ AK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQAAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGINLAQGILELHS EILVLNLKPSNM
Sbjct: 121 GKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGINLAQGILELHSKEILVLNLKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLHGVPIPN+D+IQRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLTTKDQAILGDVGIPFLLHGVPIPNSDIIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VF SSQ  VN DWQ IGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFLSSQH-VNGDWQDIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG++VGLEGET KDAFVLVRVRGIHDPVRVYAS LERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGKVVGLEGETEKDAFVLVRVRGIHDPVRVYASMLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEA+KKHS VGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR
Sbjct: 421 KEADKKHSSVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKKG VWATG+IWWILPNGCLMVKFPGILSFKEEC++YMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGNVWATGKIWWILPNGCLMVKFPGILSFKEECNSYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRP+LIAFG+FTAMKLG AFG KVGRSKVKKGQ NLVYCE+QH E
Sbjct: 541 GMVKKYQHLEDFHWSVRPILIAFGMFTAMKLGIAFG-KVGRSKVKKGQSNLVYCESQHTE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQN NNPAWIPPPVKNILFGDGV+TV R
Sbjct: 601 GQNTNNPAWIPPPVKNILFGDGVNTVTR 626

BLAST of Spg016891 vs. ExPASy TrEMBL
Match: A0A0A0LJA1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G877620 PE=4 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 581/628 (92.52%), Postives = 602/628 (95.86%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           M+ QEA SQPVPSFEYELLDGD DHVRTVVASSNY SPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MSTQEAASQPVPSFEYELLDGDTDHVRTVVASSNYSSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLED FYKCQ AK VCRLYGVSII 
Sbjct: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDRFYKCQAAKGVCRLYGVSIIG 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
           GKLCIIM FYEGS+ADKMARLK+GKLS PDVLRYGINLAQGI ELHS EILVLN+KPSNM
Sbjct: 121 GKLCIIMKFYEGSIADKMARLKDGKLSPPDVLRYGINLAQGIFELHSKEILVLNMKPSNM 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LLTTKDQAILGD+GIP+LLH VPIPN+D++QRLGTPNYMAPEQWQPEVRGPISYETDSWG
Sbjct: 181 LLTTKDQAILGDIGIPFLLHSVPIPNSDIVQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           FACC++EMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP
Sbjct: 241 FACCIIEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VFQS Q  VN DWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFQSFQH-VNGDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEG+IVGLEGET KDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGKIVGLEGETEKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEA+KKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR
Sbjct: 421 KEADKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480

Query: 481 PRFEWPRKKG-VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEW RKKG VWATG+IWWILPNGCLMVKFPGILSFKEEC++YMADPAEVE VNFSTCP
Sbjct: 481 PRFEWLRKKGSVWATGKIWWILPNGCLMVKFPGILSFKEECNSYMADPAEVEVVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQHVE 600
           GMVKKYQHLEDFHWSVRP+LIAFG+FTAMKLG AFG KVGRSKVKKGQ NLVYCE+QHVE
Sbjct: 541 GMVKKYQHLEDFHWSVRPILIAFGMFTAMKLGIAFG-KVGRSKVKKGQSNLVYCESQHVE 600

Query: 601 GQNANNPAWIPPPVKNILFGDGVSTVAR 628
           GQN NNPAWIPPPVKNILFGD V+TVAR
Sbjct: 601 GQNTNNPAWIPPPVKNILFGDSVNTVAR 626

BLAST of Spg016891 vs. ExPASy TrEMBL
Match: A0A6J1F6J8 (E3 ubiquitin-protein ligase KEG OS=Cucurbita moschata OX=3662 GN=LOC111442801 PE=4 SV=1)

HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 582/629 (92.53%), Postives = 600/629 (95.39%), Query Frame = 0

Query: 1   MAAQEAGSQPVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60
           MA QEA SQ VPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW
Sbjct: 1   MATQEAASQAVPSFEYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVW 60

Query: 61  LATHHQSTKDYDEYHEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIIS 120
           LAT HQSTKDYDEYHEVAVKMLNPVKEDHM+VV DKLEDHFYKCQG KSVCRLYGVSII 
Sbjct: 61  LATLHQSTKDYDEYHEVAVKMLNPVKEDHMKVVFDKLEDHFYKCQGGKSVCRLYGVSIID 120

Query: 121 GKLCIIMNFYEGSVADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNM 180
            KLCIIM FYEGSVADKMARLK+GKLSLPDVLRYGI+L+QGILELHS E+LVLNLKPSN+
Sbjct: 121 EKLCIIMKFYEGSVADKMARLKDGKLSLPDVLRYGISLSQGILELHSKEVLVLNLKPSNV 180

Query: 181 LLTTKDQAILGDLGIPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWG 240
           LL+TKDQAILGDLGIP+LL GVPIPNTD+IQRLGTPNYMAPEQWQPE+RGPIS ETDSWG
Sbjct: 181 LLSTKDQAILGDLGIPFLLRGVPIPNTDIIQRLGTPNYMAPEQWQPEIRGPISNETDSWG 240

Query: 241 FACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRP 300
           F CC+VEMLTGV PW GKSVDEIFHSVVRKQEKPCIPSGLPPLIEN L GCFEYDLRSRP
Sbjct: 241 FGCCVVEMLTGVPPWHGKSVDEIFHSVVRKQEKPCIPSGLPPLIENALFGCFEYDLRSRP 300

Query: 301 LMTDILHVFQSSQQVVNSDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360
           LMTDIL+VFQSS Q VN+DWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN
Sbjct: 301 LMTDILNVFQSSLQGVNNDWQAIGSSKVLNKSSATGHTEWFLSKDHLQVNDLVRSRKPLN 360

Query: 361 SCKSDNMNIPEGRIVGLEGETGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRL 420
           SCKSDNMNIPEGRIVGLE ET KDAFVLVRVRGIHDPVRV ASTLERVSFGLAAGDWIRL
Sbjct: 361 SCKSDNMNIPEGRIVGLERETEKDAFVLVRVRGIHDPVRVCASTLERVSFGLAAGDWIRL 420

Query: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILR 480
           KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKAS+LR
Sbjct: 421 KEAEKKHSPVGILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASVLR 480

Query: 481 PRFEWPRKK-GVWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCP 540
           PRFEWPRKK GVWATGRIWW+LPNGCLMVKFPGIL+FKEECDTYMADPAEVEAVNFSTCP
Sbjct: 481 PRFEWPRKKGGVWATGRIWWVLPNGCLMVKFPGILAFKEECDTYMADPAEVEAVNFSTCP 540

Query: 541 GMVKKYQHLEDFHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSKVKKGQGNLVYCETQH-V 600
           GMVKKYQHLEDFHWSVRPVL+AFGLFTAMKLG AFGKKVGRSKVKK QGNLVYCE+QH  
Sbjct: 541 GMVKKYQHLEDFHWSVRPVLVAFGLFTAMKLGIAFGKKVGRSKVKKPQGNLVYCESQHTA 600

Query: 601 EGQNANNPAWIPPPVKNILFGDGVSTVAR 628
           EGQNANNPAWIPPPVKNILF DGV TVAR
Sbjct: 601 EGQNANNPAWIPPPVKNILFRDGVGTVAR 629

BLAST of Spg016891 vs. TAIR 10
Match: AT4G32250.1 (Protein kinase superfamily protein )

HSP 1 Score: 791.2 bits (2042), Expect = 6.1e-229
Identity = 382/600 (63.67%), Postives = 472/600 (78.67%), Query Frame = 0

Query: 15  EYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVWLATHHQSTKDYDEY 74
           EYE+++G+ +      A +   SP +  S +KLRHRIGRG FGDVWLATHHQST+DYDE+
Sbjct: 14  EYEIIEGESES-----ALAAGTSPWMNSSTLKLRHRIGRGPFGDVWLATHHQSTEDYDEH 73

Query: 75  HEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSV 134
           HEVA+KML P+KED  RVV+DK ED F KCQG ++VC L GVS I+GK+C++M FYEGS+
Sbjct: 74  HEVAIKMLYPIKEDQRRVVVDKFEDLFSKCQGLENVCLLRGVSSINGKICVVMKFYEGSL 133

Query: 135 ADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLG 194
            DKMARLK GKLSLPDVLRYG++LA GILELHS   L+LNLKPSN LL+  D+AILGD+G
Sbjct: 134 GDKMARLKGGKLSLPDVLRYGVDLATGILELHSKGFLILNLKPSNFLLSDNDKAILGDVG 193

Query: 195 IPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWGFACCMVEMLTGVQP 254
           IPYLL  +P+P++D+ +RLGTPNYMAPEQWQP+VRGP+S+ETDSWGF C +VEMLTGVQP
Sbjct: 194 IPYLLLSIPLPSSDMTERLGTPNYMAPEQWQPDVRGPMSFETDSWGFGCSIVEMLTGVQP 253

Query: 255 WRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRPLMTDILHVFQSSQQ 314
           W G+S DEI+  VVRKQEK  IPS +PP +EN+L GCF YDLRSRP MTDIL V +S Q 
Sbjct: 254 WSGRSADEIYDLVVRKQEKLSIPSSIPPPLENLLRGCFMYDLRSRPSMTDILLVLKSLQN 313

Query: 315 VVNSDWQAIGSSKVLNKSSAT-GHTEWFLSKDHLQVNDLVRSRKPLNSCKSDNMNIPEGR 374
                 +    S+ + KSSAT G+TEWFLSKDHLQV D VRSRKP NSCK +NM++PEG 
Sbjct: 314 SEEEQVRRGIDSREIRKSSATLGYTEWFLSKDHLQVRDTVRSRKPANSCKHENMDVPEGM 373

Query: 375 IVGLEGE-TGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRLK-EAEKKHSPVG 434
           +VGLE + T  D FVLV+V G+HDP+RV+ S LERV+ GLA+GDW+RLK   +K+HSPVG
Sbjct: 374 VVGLERDSTDPDGFVLVKVHGVHDPLRVHVSVLERVTNGLASGDWVRLKVRKDKRHSPVG 433

Query: 435 ILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILRPRFEWPRK-KG 494
           +LHSIDR GNVAV FIG+ TLWKG SSQ QMA+ + VGQFV++KA+++ PRF+W RK +G
Sbjct: 434 VLHSIDREGNVAVGFIGLPTLWKGTSSQLQMAKVYSVGQFVKLKANVVIPRFKWMRKGRG 493

Query: 495 VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCPGMVKKYQHLED 554
           +WATGRI  +LPNGCL V FPG+L F EE  +Y+ADPAEVE VNF+TC G V+KYQHLED
Sbjct: 494 IWATGRISQVLPNGCLEVDFPGMLPFGEEHGSYLADPAEVEIVNFNTCQGAVEKYQHLED 553

Query: 555 FHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSK-VKKGQGNLVYCETQHVEGQNANNPAWI 610
           FHW+VRP+LIA GL TAMKLG    KK+GRSK  K+  G+    + +  +G+ ++   W+
Sbjct: 554 FHWAVRPLLIAMGLLTAMKLGICVRKKIGRSKDGKQRDGSTGQGDCKIPDGKGSDKSKWL 608

BLAST of Spg016891 vs. TAIR 10
Match: AT4G32250.2 (Protein kinase superfamily protein )

HSP 1 Score: 791.2 bits (2042), Expect = 6.1e-229
Identity = 382/600 (63.67%), Postives = 472/600 (78.67%), Query Frame = 0

Query: 15  EYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVWLATHHQSTKDYDEY 74
           EYE+++G+ +      A +   SP +  S +KLRHRIGRG FGDVWLATHHQST+DYDE+
Sbjct: 14  EYEIIEGESES-----ALAAGTSPWMNSSTLKLRHRIGRGPFGDVWLATHHQSTEDYDEH 73

Query: 75  HEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSV 134
           HEVA+KML P+KED  RVV+DK ED F KCQG ++VC L GVS I+GK+C++M FYEGS+
Sbjct: 74  HEVAIKMLYPIKEDQRRVVVDKFEDLFSKCQGLENVCLLRGVSSINGKICVVMKFYEGSL 133

Query: 135 ADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLG 194
            DKMARLK GKLSLPDVLRYG++LA GILELHS   L+LNLKPSN LL+  D+AILGD+G
Sbjct: 134 GDKMARLKGGKLSLPDVLRYGVDLATGILELHSKGFLILNLKPSNFLLSDNDKAILGDVG 193

Query: 195 IPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWGFACCMVEMLTGVQP 254
           IPYLL  +P+P++D+ +RLGTPNYMAPEQWQP+VRGP+S+ETDSWGF C +VEMLTGVQP
Sbjct: 194 IPYLLLSIPLPSSDMTERLGTPNYMAPEQWQPDVRGPMSFETDSWGFGCSIVEMLTGVQP 253

Query: 255 WRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRPLMTDILHVFQSSQQ 314
           W G+S DEI+  VVRKQEK  IPS +PP +EN+L GCF YDLRSRP MTDIL V +S Q 
Sbjct: 254 WSGRSADEIYDLVVRKQEKLSIPSSIPPPLENLLRGCFMYDLRSRPSMTDILLVLKSLQN 313

Query: 315 VVNSDWQAIGSSKVLNKSSAT-GHTEWFLSKDHLQVNDLVRSRKPLNSCKSDNMNIPEGR 374
                 +    S+ + KSSAT G+TEWFLSKDHLQV D VRSRKP NSCK +NM++PEG 
Sbjct: 314 SEEEQVRRGIDSREIRKSSATLGYTEWFLSKDHLQVRDTVRSRKPANSCKHENMDVPEGM 373

Query: 375 IVGLEGE-TGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRLK-EAEKKHSPVG 434
           +VGLE + T  D FVLV+V G+HDP+RV+ S LERV+ GLA+GDW+RLK   +K+HSPVG
Sbjct: 374 VVGLERDSTDPDGFVLVKVHGVHDPLRVHVSVLERVTNGLASGDWVRLKVRKDKRHSPVG 433

Query: 435 ILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILRPRFEWPRK-KG 494
           +LHSIDR GNVAV FIG+ TLWKG SSQ QMA+ + VGQFV++KA+++ PRF+W RK +G
Sbjct: 434 VLHSIDREGNVAVGFIGLPTLWKGTSSQLQMAKVYSVGQFVKLKANVVIPRFKWMRKGRG 493

Query: 495 VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCPGMVKKYQHLED 554
           +WATGRI  +LPNGCL V FPG+L F EE  +Y+ADPAEVE VNF+TC G V+KYQHLED
Sbjct: 494 IWATGRISQVLPNGCLEVDFPGMLPFGEEHGSYLADPAEVEIVNFNTCQGAVEKYQHLED 553

Query: 555 FHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSK-VKKGQGNLVYCETQHVEGQNANNPAWI 610
           FHW+VRP+LIA GL TAMKLG    KK+GRSK  K+  G+    + +  +G+ ++   W+
Sbjct: 554 FHWAVRPLLIAMGLLTAMKLGICVRKKIGRSKDGKQRDGSTGQGDCKIPDGKGSDKSKWL 608

BLAST of Spg016891 vs. TAIR 10
Match: AT4G32250.3 (Protein kinase superfamily protein )

HSP 1 Score: 791.2 bits (2042), Expect = 6.1e-229
Identity = 382/600 (63.67%), Postives = 472/600 (78.67%), Query Frame = 0

Query: 15  EYELLDGDPDHVRTVVASSNYPSPRIEPSKVKLRHRIGRGVFGDVWLATHHQSTKDYDEY 74
           EYE+++G+ +      A +   SP +  S +KLRHRIGRG FGDVWLATHHQST+DYDE+
Sbjct: 14  EYEIIEGESES-----ALAAGTSPWMNSSTLKLRHRIGRGPFGDVWLATHHQSTEDYDEH 73

Query: 75  HEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSV 134
           HEVA+KML P+KED  RVV+DK ED F KCQG ++VC L GVS I+GK+C++M FYEGS+
Sbjct: 74  HEVAIKMLYPIKEDQRRVVVDKFEDLFSKCQGLENVCLLRGVSSINGKICVVMKFYEGSL 133

Query: 135 ADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLG 194
            DKMARLK GKLSLPDVLRYG++LA GILELHS   L+LNLKPSN LL+  D+AILGD+G
Sbjct: 134 GDKMARLKGGKLSLPDVLRYGVDLATGILELHSKGFLILNLKPSNFLLSDNDKAILGDVG 193

Query: 195 IPYLLHGVPIPNTDVIQRLGTPNYMAPEQWQPEVRGPISYETDSWGFACCMVEMLTGVQP 254
           IPYLL  +P+P++D+ +RLGTPNYMAPEQWQP+VRGP+S+ETDSWGF C +VEMLTGVQP
Sbjct: 194 IPYLLLSIPLPSSDMTERLGTPNYMAPEQWQPDVRGPMSFETDSWGFGCSIVEMLTGVQP 253

Query: 255 WRGKSVDEIFHSVVRKQEKPCIPSGLPPLIENVLLGCFEYDLRSRPLMTDILHVFQSSQQ 314
           W G+S DEI+  VVRKQEK  IPS +PP +EN+L GCF YDLRSRP MTDIL V +S Q 
Sbjct: 254 WSGRSADEIYDLVVRKQEKLSIPSSIPPPLENLLRGCFMYDLRSRPSMTDILLVLKSLQN 313

Query: 315 VVNSDWQAIGSSKVLNKSSAT-GHTEWFLSKDHLQVNDLVRSRKPLNSCKSDNMNIPEGR 374
                 +    S+ + KSSAT G+TEWFLSKDHLQV D VRSRKP NSCK +NM++PEG 
Sbjct: 314 SEEEQVRRGIDSREIRKSSATLGYTEWFLSKDHLQVRDTVRSRKPANSCKHENMDVPEGM 373

Query: 375 IVGLEGE-TGKDAFVLVRVRGIHDPVRVYASTLERVSFGLAAGDWIRLK-EAEKKHSPVG 434
           +VGLE + T  D FVLV+V G+HDP+RV+ S LERV+ GLA+GDW+RLK   +K+HSPVG
Sbjct: 374 VVGLERDSTDPDGFVLVKVHGVHDPLRVHVSVLERVTNGLASGDWVRLKVRKDKRHSPVG 433

Query: 435 ILHSIDRVGNVAVAFIGVETLWKGNSSQFQMAESFCVGQFVRIKASILRPRFEWPRK-KG 494
           +LHSIDR GNVAV FIG+ TLWKG SSQ QMA+ + VGQFV++KA+++ PRF+W RK +G
Sbjct: 434 VLHSIDREGNVAVGFIGLPTLWKGTSSQLQMAKVYSVGQFVKLKANVVIPRFKWMRKGRG 493

Query: 495 VWATGRIWWILPNGCLMVKFPGILSFKEECDTYMADPAEVEAVNFSTCPGMVKKYQHLED 554
           +WATGRI  +LPNGCL V FPG+L F EE  +Y+ADPAEVE VNF+TC G V+KYQHLED
Sbjct: 494 IWATGRISQVLPNGCLEVDFPGMLPFGEEHGSYLADPAEVEIVNFNTCQGAVEKYQHLED 553

Query: 555 FHWSVRPVLIAFGLFTAMKLGFAFGKKVGRSK-VKKGQGNLVYCETQHVEGQNANNPAWI 610
           FHW+VRP+LIA GL TAMKLG    KK+GRSK  K+  G+    + +  +G+ ++   W+
Sbjct: 554 FHWAVRPLLIAMGLLTAMKLGICVRKKIGRSKDGKQRDGSTGQGDCKIPDGKGSDKSKWL 608

BLAST of Spg016891 vs. TAIR 10
Match: AT5G13530.1 (protein kinases;ubiquitin-protein ligases )

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-33
Identity = 82/259 (31.66%), Postives = 133/259 (51.35%), Query Frame = 0

Query: 75  HEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSV 134
           H VAVK +   ++  +  +  +LE         ++VC  +GV  + G LC++M+   GSV
Sbjct: 171 HRVAVKKMTLTEDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSV 230

Query: 135 ADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLG 194
             +M R  EG+L+L  +LRYG ++A+G+ ELH+  ++ +N+KPSN+LL     A++ D G
Sbjct: 231 QSEMQR-NEGRLTLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYG 290

Query: 195 IPYLLHGVPIPN-------------TDVIQRLGTPNYMAPEQWQP------EVRGPISYE 254
           +  +L                    TD +    +P+Y APE W P      E    +S E
Sbjct: 291 LAPILKKPTCQKTRPEFDSSKVTLYTDCVTL--SPHYTAPEAWGPVKKLFWEDASGVSPE 350

Query: 255 TDSWGFACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKP-----CIPSGLPPLIENVLLG 310
           +D+W F C +VEM TG  PW G S +EIF +VV+ ++ P      +  G+P  +  ++  
Sbjct: 351 SDAWSFGCTLVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGE 410


HSP 2 Score: 62.8 bits (151), Expect = 1.2e-09
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 0

Query: 415  GDWIRLKEAE---KKHSP--VGILHSI-----DRVGNVAVAFIGVETLWKGNSSQFQMAE 474
            G+W+RL+E     K   P  VG++H +     +  G  +V+F G +  W G +S  + A+
Sbjct: 1372 GEWVRLREGVSCWKSVGPGSVGVVHGVGYEGDEWDGTTSVSFCGEQERWAGPTSHLEKAK 1431

Query: 475  SFCVGQFVRIKASILRPRFEWPRKKGVWATGRIWWILPNGCLMVKFPGILSFKEECDTYM 534
               VGQ  R+K ++ +PRF W       + G I  I  +G L +  P          T+M
Sbjct: 1432 KLVVGQKTRVKLAVKQPRFGWSGHSH-GSVGTISAIDADGKLRIYTPA------GSKTWM 1491

BLAST of Spg016891 vs. TAIR 10
Match: AT5G13530.2 (protein kinases;ubiquitin-protein ligases )

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-33
Identity = 82/259 (31.66%), Postives = 133/259 (51.35%), Query Frame = 0

Query: 75  HEVAVKMLNPVKEDHMRVVLDKLEDHFYKCQGAKSVCRLYGVSIISGKLCIIMNFYEGSV 134
           H VAVK +   ++  +  +  +LE         ++VC  +GV  + G LC++M+   GSV
Sbjct: 171 HRVAVKKMTLTEDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSV 230

Query: 135 ADKMARLKEGKLSLPDVLRYGINLAQGILELHSMEILVLNLKPSNMLLTTKDQAILGDLG 194
             +M R  EG+L+L  +LRYG ++A+G+ ELH+  ++ +N+KPSN+LL     A++ D G
Sbjct: 231 QSEMQR-NEGRLTLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYG 290

Query: 195 IPYLLHGVPIPN-------------TDVIQRLGTPNYMAPEQWQP------EVRGPISYE 254
           +  +L                    TD +    +P+Y APE W P      E    +S E
Sbjct: 291 LAPILKKPTCQKTRPEFDSSKVTLYTDCVTL--SPHYTAPEAWGPVKKLFWEDASGVSPE 350

Query: 255 TDSWGFACCMVEMLTGVQPWRGKSVDEIFHSVVRKQEKP-----CIPSGLPPLIENVLLG 310
           +D+W F C +VEM TG  PW G S +EIF +VV+ ++ P      +  G+P  +  ++  
Sbjct: 351 SDAWSFGCTLVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGE 410


HSP 2 Score: 62.8 bits (151), Expect = 1.2e-09
Identity = 40/129 (31.01%), Postives = 63/129 (48.84%), Query Frame = 0

Query: 415  GDWIRLKEAE---KKHSP--VGILHSI-----DRVGNVAVAFIGVETLWKGNSSQFQMAE 474
            G+W+RL+E     K   P  VG++H +     +  G  +V+F G +  W G +S  + A+
Sbjct: 1371 GEWVRLREGVSCWKSVGPGSVGVVHGVGYEGDEWDGTTSVSFCGEQERWAGPTSHLEKAK 1430

Query: 475  SFCVGQFVRIKASILRPRFEWPRKKGVWATGRIWWILPNGCLMVKFPGILSFKEECDTYM 534
               VGQ  R+K ++ +PRF W       + G I  I  +G L +  P          T+M
Sbjct: 1431 KLVVGQKTRVKLAVKQPRFGWSGHSH-GSVGTISAIDADGKLRIYTPA------GSKTWM 1490

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898209.10.0e+0094.59probable serine/threonine-protein kinase DDB_G0292354 [Benincasa hispida][more]
KAA0038817.10.0e+0093.31E3 ubiquitin-protein ligase KEG [Cucumis melo var. makuwa][more]
XP_008466423.10.0e+0093.31PREDICTED: E3 ubiquitin-protein ligase KEG [Cucumis melo] >XP_008466424.1 PREDIC... [more]
XP_004136355.10.0e+0092.52E3 ubiquitin-protein ligase KEG [Cucumis sativus] >XP_011652477.1 E3 ubiquitin-p... [more]
XP_023535362.10.0e+0092.69E3 ubiquitin-protein ligase KEG [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FY481.6e-3231.66E3 ubiquitin-protein ligase KEG OS=Arabidopsis thaliana OX=3702 GN=KEG PE=1 SV=2[more]
O225582.3e-1825.45Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
F4JTP56.6e-1824.32Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
Q54RZ74.7e-1625.99Probable serine/threonine-protein kinase DDB_G0282895 OS=Dictyostelium discoideu... [more]
G5EFD22.2e-1325.37Kinase suppressor of Ras A OS=Caenorhabditis elegans OX=6239 GN=ksr-1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TB540.0e+0093.31E3 ubiquitin-protein ligase KEG OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3CR790.0e+0093.31E3 ubiquitin-protein ligase KEG OS=Cucumis melo OX=3656 GN=LOC103503837 PE=4 SV=... [more]
A0A5D3E7320.0e+0093.31E3 ubiquitin-protein ligase KEG OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0LJA10.0e+0092.52Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G877... [more]
A0A6J1F6J80.0e+0092.53E3 ubiquitin-protein ligase KEG OS=Cucurbita moschata OX=3662 GN=LOC111442801 PE... [more]
Match NameE-valueIdentityDescription
AT4G32250.16.1e-22963.67Protein kinase superfamily protein [more]
AT4G32250.26.1e-22963.67Protein kinase superfamily protein [more]
AT4G32250.36.1e-22963.67Protein kinase superfamily protein [more]
AT5G13530.11.1e-3331.66protein kinases;ubiquitin-protein ligases [more]
AT5G13530.21.1e-3331.66protein kinases;ubiquitin-protein ligases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 46..307
e-value: 6.9E-31
score: 107.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 20..130
e-value: 2.2E-10
score: 42.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 131..357
e-value: 7.1E-37
score: 128.8
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 41..312
e-value: 1.8E-9
score: 34.8
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 39..296
e-value: 9.7E-17
score: 58.3
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 50..270
e-value: 1.4E-7
score: 27.2
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 38..268
e-value: 6.8E-8
score: 29.9
NoneNo IPR availablePANTHERPTHR47209OS06G0639500 PROTEINcoord: 1..624
NoneNo IPR availablePANTHERPTHR47209:SF1OS06G0639500 PROTEINcoord: 1..624
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 51..80
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 45..310
score: 27.933744
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 43..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016891.1Spg016891.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009704 de-etiolation
biological_process GO:1904216 positive regulation of protein import into chloroplast stroma
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0031359 integral component of chloroplast outer membrane
cellular_component GO:0010006 Toc complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity