Spg016840 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016840
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGDSL esterase/lipase
Locationscaffold9: 39967707 .. 39970174 (+)
RNA-Seq ExpressionSpg016840
SyntenySpg016840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACCCCAAAACTTTCCCCAAAAGTACTCTCTCCCACTGGTTTACGCTCTCGCACAGAAACCTAACAAGGGAAGAAGAAGAAGAAGAACAGAGGGCGGCGGTGGGTTAATGGCAACTCGCGATACGGCGGAGCTGACACCGGAGGCGGCGGCGGATGCGGAGGCCCATCCCTATGCATTTCATGTATCTGGACCTCGCAATGTTTCTTCTCCTAATTGGCGAGATCTCATTAATTCCAGTTGGTAACTTCACTCTCTCTTCCATTTCTGTTTGTTCCAGATTTCTTCTGATTCTGGTTTCTTCTTCATTCATGGTTAATTCATCACGAAACTAAAAAGCTTAAGCTGATTAGTTATGGATAATTCAATCGCATATCAATACTTTAACACTGATCTGTGGGCATCAAATTCGCACGAGACTCGATGAGTGGTTATCGTTAAGAGGAAATGACTCGACATGGTTCAAACATGGAGCTTCTGCTATTAAATCTTCATTAAATCTAAGAGTTTAAGTGGATAGGTAATGGTAAATTTGATCAGGAGGAAATGACTTGACATGATTCAAACATAGAGGCTCCTGCTATGATACATGTTAAATCTTCACTAAATCCAAGAGTTTAAGTGGATAGGTTACAGTAAATTTGATCTTTAGCATGGATTGATTGATTTAAAACGATCGGTCACGGATAGAAGTTGAATTCTCATGTACATGTAAGATGTTTGTGGGATACTTGAGATCCATGATCATTGAAGCTGAAGGTAGATGGATTTGAAATGGTTCTGAGACTGGCTTTTATTGGGAAACTATGTGCTATGTTTTCCTTAGATTGATTGAAATTTAGTCATGAAACTTAATTTTATAATGCCTCATTCTAACATTATGCATGTTGAATCTGATGAGGAATTTTGCAAAATCTGCAGGAAGGATGGGAATTACAAAAGAACTGTTATTGCCTGTTTTATTCAAGCAGTTTACTTGCTTGAACTAGACAGACAAGAGAACAGAAGCAACAAAGAGAATGCTCTGGCTCCCAAATGGTGGATTCCCTTCAAATACAAGTTAGTTCAAACCTTGATTGATGAAAGAGATGGGTCCATATTTGGTGCAATACTAGAATGGGATAGGTCTGCAGCCATGGCTGACCTAGTACTCATCAGACCCAGTGGTGCACCAAGGGCTGTTTTAGCATTAAGAGGGACATTACTCAAAAGCCCGACGATCCGACGAGATATCGAGGACGACCTTCGGTTTCTAGCTTGGGAGAGCCTGAAGGGTTCTGTTAGGTTTAATGTAGCTTTGCAGGCATTGAAATCAGTTGCTGAGAGTTTTGGAAGCAACAATGTTTGTATTGCTGGTCATTCCTTAGGTGCTGGGTTTGCTCTGCAGGTGGGGAAGGCCTTAGCTAAAGAAGGAATCTTTGTTGAAACTCACTTGTTCAATCCTCCTTCAGTTTCATTAGCAATGAGCTTGAGGAACATAGGGGAAAAGGCAGAGTTTGCTTGGAAGAGATTCAAATCAATGCTTCCTTCAGGCAGTGAAACAACAGAGAACAGCAATATTGAAGGAGAAAAAGCATCTGCAGCAGCTGGTTTAAAGAGTTGGGTATCTAATCTAAACAGGTTGAAGAATCCTGGGGTGGGAATTGGGAAGTGGGTCCCACATCTATATGTAAATAATAGTGATTATATCTGCTGTTCATATACTGAATCTGATCAAGTAGAGAAAAGCAATGAAGGGAAAGAAAATATCAATCCCACAAATGGGAATGGGAAAGCAGCAGCTGCAGCGAAGTTGTTTGTAATGTCAAAGGGAAAGCAGAAGTTTCTTGAGGCTCATGGATTGGAACAATGGTGGTCTGATGATTTGCAGCTTCAGCTAGCTCTCCATGACAGCAAGCTCATAAGCAGGCAGCTTAAGTCCTTGTATGCTCTTTCATCAGCACAGCCAACACAGGTAAATCCCAGCAGGTGAAGTTCACTTGCTGGAATTTTGTGCAAATAACTCATTTTCGACTTCGGGATATGTCGGTATAGTTCTTCTGATGTCATGTCTCATGTCTGTTGCTTCATTCGGATTGTGTTCAACATGAATATATGAGGTCTTCCCTTGGTGATTCTTTTCAACCCTGTTTGGTTGAGCAAATCAACCTACAGTGTATCTCATTAATTTAACAGCAAGTGAACAAAACAGATACAGATATATCACCATCAAATTAACTTCTGGATCCTTTTATTGTCAAAAAAAAAAAAAAAACTTCTGGATCCTTTTCGGTAAATGCAAGGTTCAACCAATTGGGAGAATGTTTTTCTTCCTATTGTTTTCTTTTGTAAATTTCTTCATATGAATGAAAAAAACTACCTGTTCTCTGAAAAATGTGCAGCATTGAACATAAACAAAACTTTCATGGCTCGGTATTTATTTTTATGAAGTATGGAGTTGAGAAAGTTGAATAAATTAATATGTCGTTG

mRNA sequence

AAACCCCAAAACTTTCCCCAAAAGTACTCTCTCCCACTGGTTTACGCTCTCGCACAGAAACCTAACAAGGGAAGAAGAAGAAGAAGAACAGAGGGCGGCGGTGGGTTAATGGCAACTCGCGATACGGCGGAGCTGACACCGGAGGCGGCGGCGGATGCGGAGGCCCATCCCTATGCATTTCATGTATCTGGACCTCGCAATGTTTCTTCTCCTAATTGGCGAGATCTCATTAATTCCAGTTGGAAGGATGGGAATTACAAAAGAACTGTTATTGCCTGTTTTATTCAAGCAGTTTACTTGCTTGAACTAGACAGACAAGAGAACAGAAGCAACAAAGAGAATGCTCTGGCTCCCAAATGGTGGATTCCCTTCAAATACAAGTTAGTTCAAACCTTGATTGATGAAAGAGATGGGTCCATATTTGGTGCAATACTAGAATGGGATAGGTCTGCAGCCATGGCTGACCTAGTACTCATCAGACCCAGTGGTGCACCAAGGGCTGTTTTAGCATTAAGAGGGACATTACTCAAAAGCCCGACGATCCGACGAGATATCGAGGACGACCTTCGGTTTCTAGCTTGGGAGAGCCTGAAGGGTTCTGTTAGGTTTAATGTAGCTTTGCAGGCATTGAAATCAGTTGCTGAGAGTTTTGGAAGCAACAATGTTTGTATTGCTGGTCATTCCTTAGGTGCTGGGTTTGCTCTGCAGGTGGGGAAGGCCTTAGCTAAAGAAGGAATCTTTGTTGAAACTCACTTGTTCAATCCTCCTTCAGTTTCATTAGCAATGAGCTTGAGGAACATAGGGGAAAAGGCAGAGTTTGCTTGGAAGAGATTCAAATCAATGCTTCCTTCAGGCAGTGAAACAACAGAGAACAGCAATATTGAAGGAGAAAAAGCATCTGCAGCAGCTGGTTTAAAGAGTTGGGTATCTAATCTAAACAGGTTGAAGAATCCTGGGGTGGGAATTGGGAAGTGGGTCCCACATCTATATGTAAATAATAGTGATTATATCTGCTGTTCATATACTGAATCTGATCAAGTAGAGAAAAGCAATGAAGGGAAAGAAAATATCAATCCCACAAATGGGAATGGGAAAGCAGCAGCTGCAGCGAAGTTGTTTGTAATGTCAAAGGGAAAGCAGAAGTTTCTTGAGGCTCATGGATTGGAACAATGGTGGTCTGATGATTTGCAGCTTCAGCTAGCTCTCCATGACAGCAAGCTCATAAGCAGGCAGCTTAAGTCCTTGTATGCTCTTTCATCAGCACAGCCAACACAGGTAAATCCCAGCAGGTGA

Coding sequence (CDS)

AAACCCCAAAACTTTCCCCAAAAGTACTCTCTCCCACTGGTTTACGCTCTCGCACAGAAACCTAACAAGGGAAGAAGAAGAAGAAGAACAGAGGGCGGCGGTGGGTTAATGGCAACTCGCGATACGGCGGAGCTGACACCGGAGGCGGCGGCGGATGCGGAGGCCCATCCCTATGCATTTCATGTATCTGGACCTCGCAATGTTTCTTCTCCTAATTGGCGAGATCTCATTAATTCCAGTTGGAAGGATGGGAATTACAAAAGAACTGTTATTGCCTGTTTTATTCAAGCAGTTTACTTGCTTGAACTAGACAGACAAGAGAACAGAAGCAACAAAGAGAATGCTCTGGCTCCCAAATGGTGGATTCCCTTCAAATACAAGTTAGTTCAAACCTTGATTGATGAAAGAGATGGGTCCATATTTGGTGCAATACTAGAATGGGATAGGTCTGCAGCCATGGCTGACCTAGTACTCATCAGACCCAGTGGTGCACCAAGGGCTGTTTTAGCATTAAGAGGGACATTACTCAAAAGCCCGACGATCCGACGAGATATCGAGGACGACCTTCGGTTTCTAGCTTGGGAGAGCCTGAAGGGTTCTGTTAGGTTTAATGTAGCTTTGCAGGCATTGAAATCAGTTGCTGAGAGTTTTGGAAGCAACAATGTTTGTATTGCTGGTCATTCCTTAGGTGCTGGGTTTGCTCTGCAGGTGGGGAAGGCCTTAGCTAAAGAAGGAATCTTTGTTGAAACTCACTTGTTCAATCCTCCTTCAGTTTCATTAGCAATGAGCTTGAGGAACATAGGGGAAAAGGCAGAGTTTGCTTGGAAGAGATTCAAATCAATGCTTCCTTCAGGCAGTGAAACAACAGAGAACAGCAATATTGAAGGAGAAAAAGCATCTGCAGCAGCTGGTTTAAAGAGTTGGGTATCTAATCTAAACAGGTTGAAGAATCCTGGGGTGGGAATTGGGAAGTGGGTCCCACATCTATATGTAAATAATAGTGATTATATCTGCTGTTCATATACTGAATCTGATCAAGTAGAGAAAAGCAATGAAGGGAAAGAAAATATCAATCCCACAAATGGGAATGGGAAAGCAGCAGCTGCAGCGAAGTTGTTTGTAATGTCAAAGGGAAAGCAGAAGTTTCTTGAGGCTCATGGATTGGAACAATGGTGGTCTGATGATTTGCAGCTTCAGCTAGCTCTCCATGACAGCAAGCTCATAAGCAGGCAGCTTAAGTCCTTGTATGCTCTTTCATCAGCACAGCCAACACAGGTAAATCCCAGCAGGTGA

Protein sequence

KPQNFPQKYSLPLVYALAQKPNKGRRRRRTEGGGGLMATRDTAELTPEAAADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAESFGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWKRFKSMLPSGSETTENSNIEGEKASAAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSDYICCSYTESDQVEKSNEGKENINPTNGNGKAAAAAKLFVMSKGKQKFLEAHGLEQWWSDDLQLQLALHDSKLISRQLKSLYALSSAQPTQVNPSR
Homology
BLAST of Spg016840 vs. NCBI nr
Match: XP_022936957.1 (GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata])

HSP 1 Score: 719.2 bits (1855), Expect = 2.2e-203
Identity = 357/396 (90.15%), Postives = 375/396 (94.70%), Query Frame = 0

Query: 37  MATRDTAELTPEAAADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQ 96
           MA+ DTAE TP AA + EAHPYAFHVSGPRNV SPNWRDLINSSWKDGNYKRTV+ACFIQ
Sbjct: 1   MASPDTAESTPAAATEEEAHPYAFHVSGPRNVISPNWRDLINSSWKDGNYKRTVVACFIQ 60

Query: 97  AVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 156
           AVYLLELDRQEN+SNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL
Sbjct: 61  AVYLLELDRQENKSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 120

Query: 157 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAES 216
           VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKSVAES
Sbjct: 121 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALKALKSVAES 180

Query: 217 FGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWK 276
           +GS+NVCIAGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAEFAWK
Sbjct: 181 YGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAEFAWK 240

Query: 277 RFKSMLPSGSETTENSNIEGEKAS-AAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSD 336
           R KSMLPSGSETT NS++EGEKAS   AG K+WV+NLNRLKNPGVGIGKWVPH+YVNNSD
Sbjct: 241 RIKSMLPSGSETTGNSSMEGEKASGGGAGFKNWVANLNRLKNPGVGIGKWVPHIYVNNSD 300

Query: 337 YICCSYTESDQVEKSNEGKENINPTNGN-GKAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 396
           YICCSYTESDQVEKSNEGKEN+NP NG    AAAAAKLFVMSKGKQKFLEAHGLEQWWSD
Sbjct: 301 YICCSYTESDQVEKSNEGKENVNPKNGKAAAAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 360

Query: 397 DLQLQLALHDSKLISRQLKSLYALSSAQPTQVNPSR 431
           DLQLQLALHDSKLISRQL+SLY +S+ QP Q NPSR
Sbjct: 361 DLQLQLALHDSKLISRQLRSLYPMSTGQPIQGNPSR 396

BLAST of Spg016840 vs. NCBI nr
Match: XP_038897095.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At4g10955 [Benincasa hispida])

HSP 1 Score: 716.5 bits (1848), Expect = 1.4e-202
Identity = 364/399 (91.23%), Postives = 376/399 (94.24%), Query Frame = 0

Query: 37  MATRDTAELTPEA--AADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACF 96
           M  RDTAELT  A  A  AEAHPYAFHVSGPRNV+SPNWRDLINSSWKDGNYKRTVIACF
Sbjct: 1   MENRDTAELTAAATTATAAEAHPYAFHVSGPRNVTSPNWRDLINSSWKDGNYKRTVIACF 60

Query: 97  IQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMA 156
           IQAVYLLELDRQENRSNKENALAPKWWIPFKYKLV+TLIDERDGSIFGAILEWDRSAAMA
Sbjct: 61  IQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVRTLIDERDGSIFGAILEWDRSAAMA 120

Query: 157 DLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVA 216
           DLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKSVA
Sbjct: 121 DLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALEALKSVA 180

Query: 217 ESFGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFA 276
           ES+GSNNVC+AGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAEFA
Sbjct: 181 ESYGSNNVCVAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAEFA 240

Query: 277 WKRFKSMLPSGSETTENSNIEGEKASAAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNS 336
           WKR KSMLPSG+ETTENSNIE EKA   AGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNS
Sbjct: 241 WKRIKSMLPSGNETTENSNIEEEKAYGGAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNS 300

Query: 337 DYICCSYTE-SDQVEKSNEGKENINPTNGNG-KAAAAAKLFVMSKGKQKFLEAHGLEQWW 396
           DYICCSYTE SDQVE+SNEGKEN+N TNG    AAAAAKLFVMSKGKQKFLEAHGLEQWW
Sbjct: 301 DYICCSYTEKSDQVEQSNEGKENVNTTNGRAVAAAAAAKLFVMSKGKQKFLEAHGLEQWW 360

Query: 397 SDDLQLQLALHDSKLISRQLKSLYALSSAQ-PTQVNPSR 431
           SDDLQLQLALHDSKLISRQLKSLY +S+AQ  TQ NPSR
Sbjct: 361 SDDLQLQLALHDSKLISRQLKSLYTISAAQXQTQGNPSR 399

BLAST of Spg016840 vs. NCBI nr
Match: KAG6591835.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714.9 bits (1844), Expect = 4.1e-202
Identity = 359/401 (89.53%), Postives = 376/401 (93.77%), Query Frame = 0

Query: 37  MATRDTAELTPEAAADA----EAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIA 96
           MA+ DTAE TP AAA A    EAHPYAFHVSGPRNV SPNWRDLINSSWKDGNYKRTV+A
Sbjct: 1   MASPDTAESTPAAAAAAATEEEAHPYAFHVSGPRNVISPNWRDLINSSWKDGNYKRTVVA 60

Query: 97  CFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA 156
           CFIQAVYLLELDRQEN+SNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA
Sbjct: 61  CFIQAVYLLELDRQENKSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA 120

Query: 157 MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKS 216
           MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKS
Sbjct: 121 MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALKALKS 180

Query: 217 VAESFGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAE 276
           VAES+GS+NVCIAGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAE
Sbjct: 181 VAESYGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAE 240

Query: 277 FAWKRFKSMLPSGSETTENSNIEGEKAS--AAAGLKSWVSNLNRLKNPGVGIGKWVPHLY 336
           FAWKR KSMLPSGSETT NS++EGEKAS    AG K+WV+NLNRLKNPGVGIGKWVPH+Y
Sbjct: 241 FAWKRIKSMLPSGSETTGNSSMEGEKASGGGGAGFKNWVANLNRLKNPGVGIGKWVPHIY 300

Query: 337 VNNSDYICCSYTESDQVEKSNEGKENINPTNGN-GKAAAAAKLFVMSKGKQKFLEAHGLE 396
           VNNSDYICCSYTESDQVEKSNEGKEN+NP NG    AAAAAKLFVMSKGKQKFLEAHGLE
Sbjct: 301 VNNSDYICCSYTESDQVEKSNEGKENVNPKNGQAAAAAAAAKLFVMSKGKQKFLEAHGLE 360

Query: 397 QWWSDDLQLQLALHDSKLISRQLKSLYALSSAQPTQVNPSR 431
           QWWSDDLQLQLALHDSKLISRQL+SLY +S+ QP Q NPSR
Sbjct: 361 QWWSDDLQLQLALHDSKLISRQLRSLYPMSTGQPIQGNPSR 401

BLAST of Spg016840 vs. NCBI nr
Match: XP_023535014.1 (GDSL esterase/lipase At4g10955 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714.1 bits (1842), Expect = 7.0e-202
Identity = 357/398 (89.70%), Postives = 375/398 (94.22%), Query Frame = 0

Query: 37  MATRDTAELTPEAAA--DAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACF 96
           MA+ DTAE TP AAA  + EAHPYAFHVSGPRNV SPNWRDLINSSWKDGNYKRTV+ACF
Sbjct: 1   MASPDTAESTPAAAAATEEEAHPYAFHVSGPRNVISPNWRDLINSSWKDGNYKRTVVACF 60

Query: 97  IQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMA 156
           IQAVYLLELDRQEN+SNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMA
Sbjct: 61  IQAVYLLELDRQENKSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMA 120

Query: 157 DLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVA 216
           DLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKSVA
Sbjct: 121 DLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALKALKSVA 180

Query: 217 ESFGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFA 276
           ES+GS+NVCIAGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAEFA
Sbjct: 181 ESYGSHNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAEFA 240

Query: 277 WKRFKSMLPSGSETTENSNIEGEKAS-AAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNN 336
           WKR KSMLPSGSETT NS++EGEKAS   AG K+WV+NLNRLKNPGVGIGKWVPH+YVNN
Sbjct: 241 WKRIKSMLPSGSETTGNSSMEGEKASGGGAGFKNWVANLNRLKNPGVGIGKWVPHIYVNN 300

Query: 337 SDYICCSYTESDQVEKSNEGKENINPTNGN-GKAAAAAKLFVMSKGKQKFLEAHGLEQWW 396
           SDYICCSYTESDQVEKSNEGKEN+NP NG    AAAAAKLFVMSKGKQKFLEAHGLEQWW
Sbjct: 301 SDYICCSYTESDQVEKSNEGKENVNPKNGKAAAAAAAAKLFVMSKGKQKFLEAHGLEQWW 360

Query: 397 SDDLQLQLALHDSKLISRQLKSLYALSSAQPTQVNPSR 431
           SDDLQLQLALHDSKLISRQL+SLY +S+ QP Q NP R
Sbjct: 361 SDDLQLQLALHDSKLISRQLRSLYPMSTGQPIQGNPGR 398

BLAST of Spg016840 vs. NCBI nr
Match: XP_022936956.1 (GDSL esterase/lipase At4g10955 isoform X1 [Cucurbita moschata])

HSP 1 Score: 713.4 bits (1840), Expect = 1.2e-201
Identity = 354/393 (90.08%), Postives = 372/393 (94.66%), Query Frame = 0

Query: 37  MATRDTAELTPEAAADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQ 96
           MA+ DTAE TP AA + EAHPYAFHVSGPRNV SPNWRDLINSSWKDGNYKRTV+ACFIQ
Sbjct: 1   MASPDTAESTPAAATEEEAHPYAFHVSGPRNVISPNWRDLINSSWKDGNYKRTVVACFIQ 60

Query: 97  AVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 156
           AVYLLELDRQEN+SNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL
Sbjct: 61  AVYLLELDRQENKSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 120

Query: 157 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAES 216
           VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKSVAES
Sbjct: 121 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALKALKSVAES 180

Query: 217 FGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWK 276
           +GS+NVCIAGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAEFAWK
Sbjct: 181 YGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAEFAWK 240

Query: 277 RFKSMLPSGSETTENSNIEGEKAS-AAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSD 336
           R KSMLPSGSETT NS++EGEKAS   AG K+WV+NLNRLKNPGVGIGKWVPH+YVNNSD
Sbjct: 241 RIKSMLPSGSETTGNSSMEGEKASGGGAGFKNWVANLNRLKNPGVGIGKWVPHIYVNNSD 300

Query: 337 YICCSYTESDQVEKSNEGKENINPTNGN-GKAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 396
           YICCSYTESDQVEKSNEGKEN+NP NG    AAAAAKLFVMSKGKQKFLEAHGLEQWWSD
Sbjct: 301 YICCSYTESDQVEKSNEGKENVNPKNGKAAAAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 360

Query: 397 DLQLQLALHDSKLISRQLKSLYALSSAQPTQVN 428
           DLQLQLALHDSKLISRQL+SLY +S+ QP Q N
Sbjct: 361 DLQLQLALHDSKLISRQLRSLYPMSTGQPIQGN 393

BLAST of Spg016840 vs. ExPASy Swiss-Prot
Match: Q680C0 (GDSL esterase/lipase At4g10955 OS=Arabidopsis thaliana OX=3702 GN=At4g10955 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 5.7e-37
Identity = 107/365 (29.32%), Postives = 174/365 (47.67%), Query Frame = 0

Query: 60  FHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQAVYLLELDRQENRSNKENALAPK 119
           F ++GP +++S          W + +++R+V    +Q +Y+ E DRQ  R   E AL+P 
Sbjct: 9   FSLTGPLHLTS--------IDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPI 68

Query: 120 WWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSP 179
           W   F ++L++  +D+ D SIFG I E+     ++  V      +PR V+A RGT+ K  
Sbjct: 69  WSEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVK-SMEFSPRFVIAFRGTVTKVD 128

Query: 180 TIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAESFGSNNVCIAGHSLGAGFALQVGK 239
           +I RDIE D+  +    L  + RF +A+QA++++  S G ++V +AGHSLGA  AL  GK
Sbjct: 129 SISRDIEHDIHVIR-NGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGK 188

Query: 240 ALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWKRFKSMLPSGSETTENSNIEGEKA 299
            +A+ G F E   FNPP +S  +         +   KR K  +         S I    A
Sbjct: 189 TIARTGFFPECFAFNPPFLSAPIE--------KIKDKRIKHGI-----RIAGSVITAGLA 248

Query: 300 SAAAGLKSWVSNLNRL---KNPGVGIGKWVPHLYVNNSDYICCSYT----ESDQVEKSNE 359
            A    + +  N   L    +P   +  W P LYVN  D++C  Y       +++E+   
Sbjct: 249 LAKKATQHYSQNDRALPAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGI 308

Query: 360 G-KENINPTNGNG----------KAAAAAKLFVMSKGKQKFLEAHGLEQWWSDDLQLQLA 407
           G  E +   +  G              ++ L V     + F +AHG+ QWW +D + +  
Sbjct: 309 GFVERVATQHSLGGMLLGGQEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETK 350

BLAST of Spg016840 vs. ExPASy TrEMBL
Match: A0A6J1F9S8 (GDSL esterase/lipase At4g10955 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443389 PE=4 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 1.1e-203
Identity = 357/396 (90.15%), Postives = 375/396 (94.70%), Query Frame = 0

Query: 37  MATRDTAELTPEAAADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQ 96
           MA+ DTAE TP AA + EAHPYAFHVSGPRNV SPNWRDLINSSWKDGNYKRTV+ACFIQ
Sbjct: 1   MASPDTAESTPAAATEEEAHPYAFHVSGPRNVISPNWRDLINSSWKDGNYKRTVVACFIQ 60

Query: 97  AVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 156
           AVYLLELDRQEN+SNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL
Sbjct: 61  AVYLLELDRQENKSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 120

Query: 157 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAES 216
           VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKSVAES
Sbjct: 121 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALKALKSVAES 180

Query: 217 FGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWK 276
           +GS+NVCIAGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAEFAWK
Sbjct: 181 YGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAEFAWK 240

Query: 277 RFKSMLPSGSETTENSNIEGEKAS-AAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSD 336
           R KSMLPSGSETT NS++EGEKAS   AG K+WV+NLNRLKNPGVGIGKWVPH+YVNNSD
Sbjct: 241 RIKSMLPSGSETTGNSSMEGEKASGGGAGFKNWVANLNRLKNPGVGIGKWVPHIYVNNSD 300

Query: 337 YICCSYTESDQVEKSNEGKENINPTNGN-GKAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 396
           YICCSYTESDQVEKSNEGKEN+NP NG    AAAAAKLFVMSKGKQKFLEAHGLEQWWSD
Sbjct: 301 YICCSYTESDQVEKSNEGKENVNPKNGKAAAAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 360

Query: 397 DLQLQLALHDSKLISRQLKSLYALSSAQPTQVNPSR 431
           DLQLQLALHDSKLISRQL+SLY +S+ QP Q NPSR
Sbjct: 361 DLQLQLALHDSKLISRQLRSLYPMSTGQPIQGNPSR 396

BLAST of Spg016840 vs. ExPASy TrEMBL
Match: A0A6J1F8X6 (GDSL esterase/lipase At4g10955 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443389 PE=4 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 5.8e-202
Identity = 354/393 (90.08%), Postives = 372/393 (94.66%), Query Frame = 0

Query: 37  MATRDTAELTPEAAADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQ 96
           MA+ DTAE TP AA + EAHPYAFHVSGPRNV SPNWRDLINSSWKDGNYKRTV+ACFIQ
Sbjct: 1   MASPDTAESTPAAATEEEAHPYAFHVSGPRNVISPNWRDLINSSWKDGNYKRTVVACFIQ 60

Query: 97  AVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 156
           AVYLLELDRQEN+SNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL
Sbjct: 61  AVYLLELDRQENKSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADL 120

Query: 157 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAES 216
           VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKSVAES
Sbjct: 121 VLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALKALKSVAES 180

Query: 217 FGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWK 276
           +GS+NVCIAGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAEFAWK
Sbjct: 181 YGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAEFAWK 240

Query: 277 RFKSMLPSGSETTENSNIEGEKAS-AAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSD 336
           R KSMLPSGSETT NS++EGEKAS   AG K+WV+NLNRLKNPGVGIGKWVPH+YVNNSD
Sbjct: 241 RIKSMLPSGSETTGNSSMEGEKASGGGAGFKNWVANLNRLKNPGVGIGKWVPHIYVNNSD 300

Query: 337 YICCSYTESDQVEKSNEGKENINPTNGN-GKAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 396
           YICCSYTESDQVEKSNEGKEN+NP NG    AAAAAKLFVMSKGKQKFLEAHGLEQWWSD
Sbjct: 301 YICCSYTESDQVEKSNEGKENVNPKNGKAAAAAAAAKLFVMSKGKQKFLEAHGLEQWWSD 360

Query: 397 DLQLQLALHDSKLISRQLKSLYALSSAQPTQVN 428
           DLQLQLALHDSKLISRQL+SLY +S+ QP Q N
Sbjct: 361 DLQLQLALHDSKLISRQLRSLYPMSTGQPIQGN 393

BLAST of Spg016840 vs. ExPASy TrEMBL
Match: A0A6J1IGP6 (GDSL esterase/lipase At4g10955 OS=Cucurbita maxima OX=3661 GN=LOC111477067 PE=4 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.9e-201
Identity = 356/397 (89.67%), Postives = 373/397 (93.95%), Query Frame = 0

Query: 37  MATRDTAELTPEAAA-DAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFI 96
           MA+ DTAE TP AAA + EAHPYAFHVSGPRNV SPNWRDLINSSWKDGNYKRTV+ACFI
Sbjct: 1   MASPDTAESTPAAAATEEEAHPYAFHVSGPRNVISPNWRDLINSSWKDGNYKRTVVACFI 60

Query: 97  QAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMAD 156
           QAVYLLELDRQEN+SNKENALAPKWWIPFKYKL QTLIDERDGSIFGAILEWDRSAAMAD
Sbjct: 61  QAVYLLELDRQENKSNKENALAPKWWIPFKYKLAQTLIDERDGSIFGAILEWDRSAAMAD 120

Query: 157 LVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAE 216
           LVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKSVAE
Sbjct: 121 LVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALKALKSVAE 180

Query: 217 SFGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAW 276
           S+GS+NVCIAGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAEFAW
Sbjct: 181 SYGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAEFAW 240

Query: 277 KRFKSMLPSGSETTENSNIEGEKAS-AAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNS 336
           KR KSMLPSGSETT NS++EGEKAS   AG KSWV+NLNRLKNPGVGIGKWVPH+YVNNS
Sbjct: 241 KRIKSMLPSGSETTGNSSMEGEKASGGGAGFKSWVANLNRLKNPGVGIGKWVPHIYVNNS 300

Query: 337 DYICCSYTESDQVEKSNEGKENINPTNGN-GKAAAAAKLFVMSKGKQKFLEAHGLEQWWS 396
           DYICCSYTESDQVEKSNE KEN+NP  G    AAAAAKLFVMSKGKQKFLEAHGLEQWWS
Sbjct: 301 DYICCSYTESDQVEKSNEEKENVNPKKGKAAAAAAAAKLFVMSKGKQKFLEAHGLEQWWS 360

Query: 397 DDLQLQLALHDSKLISRQLKSLYALSSAQPTQVNPSR 431
           DDLQLQLALHDSKLISRQL+SLY +S+ QP Q NPSR
Sbjct: 361 DDLQLQLALHDSKLISRQLRSLYPMSTGQPIQGNPSR 397

BLAST of Spg016840 vs. ExPASy TrEMBL
Match: A0A5D3CGI8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold504G00550 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.3e-198
Identity = 355/393 (90.33%), Postives = 368/393 (93.64%), Query Frame = 0

Query: 37  MATRDTAELTP----EAAADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIA 96
           MA  D AELTP     A A AE+HPYAFHVSGPRNV+SPNWRDLINSSWKDGNYKRTV+A
Sbjct: 1   MANCDAAELTPAKTTTALAAAESHPYAFHVSGPRNVTSPNWRDLINSSWKDGNYKRTVMA 60

Query: 97  CFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA 156
           CFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA
Sbjct: 61  CFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA 120

Query: 157 MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKS 216
           MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKS
Sbjct: 121 MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALEALKS 180

Query: 217 VAESFGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAE 276
           VAES+GSNNVC+AGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAE
Sbjct: 181 VAESYGSNNVCVAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAE 240

Query: 277 FAWKRFKSMLPSGSETTEN-SNIEGEKASAAAGLKSWVSNLNRLKNPGVGIGKWVPHLYV 336
           FAWKR KSM+PSGSETTEN SNIE EK+    GLKSWVSNLNRLKNPGVGIGKWVPHLYV
Sbjct: 241 FAWKRIKSMIPSGSETTENSSNIEEEKSYGVTGLKSWVSNLNRLKNPGVGIGKWVPHLYV 300

Query: 337 NNSDYICCSYTE-SDQVEKSNEGKENINPTNGNG-KAAAAAKLFVMSKGKQKFLEAHGLE 396
           NNSDYICCSYTE SDQVEKSNEGKEN+N TNG    AAAAAKLFVMSKGKQKFLEAHGLE
Sbjct: 301 NNSDYICCSYTEKSDQVEKSNEGKENVNTTNGRAVAAAAAAKLFVMSKGKQKFLEAHGLE 360

Query: 397 QWWSDDLQLQLALHDSKLISRQLKSLYALSSAQ 423
           QWWSDDLQLQLALHDSKLISRQLKSLY +S+ Q
Sbjct: 361 QWWSDDLQLQLALHDSKLISRQLKSLYTISAPQ 393

BLAST of Spg016840 vs. ExPASy TrEMBL
Match: A0A1S3CQD4 (GDSL esterase/lipase At4g10955 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503049 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.3e-198
Identity = 355/393 (90.33%), Postives = 368/393 (93.64%), Query Frame = 0

Query: 37  MATRDTAELTP----EAAADAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIA 96
           MA  D AELTP     A A AE+HPYAFHVSGPRNV+SPNWRDLINSSWKDGNYKRTV+A
Sbjct: 1   MANCDAAELTPAKTTTALAAAESHPYAFHVSGPRNVTSPNWRDLINSSWKDGNYKRTVMA 60

Query: 97  CFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA 156
           CFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA
Sbjct: 61  CFIQAVYLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAA 120

Query: 157 MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKS 216
           MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVAL+ALKS
Sbjct: 121 MADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALEALKS 180

Query: 217 VAESFGSNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAE 276
           VAES+GSNNVC+AGHSLGAGFALQVGKALAKEGI+VETHLFNPPSVS+AMSLRNIGEKAE
Sbjct: 181 VAESYGSNNVCVAGHSLGAGFALQVGKALAKEGIYVETHLFNPPSVSVAMSLRNIGEKAE 240

Query: 277 FAWKRFKSMLPSGSETTEN-SNIEGEKASAAAGLKSWVSNLNRLKNPGVGIGKWVPHLYV 336
           FAWKR KSM+PSGSETTEN SNIE EK+    GLKSWVSNLNRLKNPGVGIGKWVPHLYV
Sbjct: 241 FAWKRIKSMIPSGSETTENSSNIEEEKSYGVTGLKSWVSNLNRLKNPGVGIGKWVPHLYV 300

Query: 337 NNSDYICCSYTE-SDQVEKSNEGKENINPTNGNG-KAAAAAKLFVMSKGKQKFLEAHGLE 396
           NNSDYICCSYTE SDQVEKSNEGKEN+N TNG    AAAAAKLFVMSKGKQKFLEAHGLE
Sbjct: 301 NNSDYICCSYTEKSDQVEKSNEGKENVNTTNGRAVAAAAAAKLFVMSKGKQKFLEAHGLE 360

Query: 397 QWWSDDLQLQLALHDSKLISRQLKSLYALSSAQ 423
           QWWSDDLQLQLALHDSKLISRQLKSLY +S+ Q
Sbjct: 361 QWWSDDLQLQLALHDSKLISRQLKSLYTISAPQ 393

BLAST of Spg016840 vs. TAIR 10
Match: AT5G50890.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 537.3 bits (1383), Expect = 1.1e-152
Identity = 272/385 (70.65%), Postives = 318/385 (82.60%), Query Frame = 0

Query: 42  TAELTPEAAA---DAEAHPYAFHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQAV 101
           ++ +TP+      D   HPYAFHVSGPRNV+SPNWRDLINSSWKD NYKRTV+ACFIQA 
Sbjct: 25  SSPMTPKTVTVKEDETHHPYAFHVSGPRNVASPNWRDLINSSWKDPNYKRTVMACFIQAA 84

Query: 102 YLLELDRQENRSNKENALAPKWWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADLVL 161
           YLLELDRQENR N++NALAPKWWIPFKYKL QTLIDERDGSIFGA+LEWDR+AAMADLV+
Sbjct: 85  YLLELDRQENR-NEQNALAPKWWIPFKYKLSQTLIDERDGSIFGAVLEWDRAAAMADLVV 144

Query: 162 IRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAESFG 221
           IRPSGAP+AVLALRGT+LKS T+RRDIEDDLRFLAWESLKGSVRFNVAL+AL+SVA  +G
Sbjct: 145 IRPSGAPKAVLALRGTILKSLTMRRDIEDDLRFLAWESLKGSVRFNVALEALQSVATRYG 204

Query: 222 SNNVCIAGHSLGAGFALQVGKALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWKRF 281
           S+NVC+ GHSLGAGFALQVGK+LAKEG+FV+ HLFNPPS+S+AMSLRNI EKA  AWKR 
Sbjct: 205 SSNVCVVGHSLGAGFALQVGKSLAKEGLFVDAHLFNPPSISVAMSLRNISEKAGVAWKRL 264

Query: 282 KSMLPSGSETTENSNIEGE--KASAAAGLKSWV-SNLNRLKNPGVGIGKWVPHLYVNNSD 341
            SMLP   E    ++ EG+   ++ ++G ++WV S     + P V + KWVPHLYVN+SD
Sbjct: 265 MSMLPPKHEPLIQNSNEGQVSPSNVSSGFRNWVPSFYGSNQKPTVDLRKWVPHLYVNDSD 324

Query: 342 YICCSYTESDQVEKSNE--GKENINPTNGNGKAAAAAKLFVMSKGKQKFLEAHGLEQWWS 401
           YICC YT+ D V +  E   KEN +P   N    AAAKLFVMSKGKQKF EAHGLEQWWS
Sbjct: 325 YICCHYTDQDGVAEKREMNNKENNSPV-VNTNFQAAAKLFVMSKGKQKFHEAHGLEQWWS 384

Query: 402 DDLQLQLALHDSKLISRQLKSLYAL 419
           D+L+LQ A+H S+LIS+QLKSLY++
Sbjct: 385 DNLELQSAIHSSRLISQQLKSLYSI 407

BLAST of Spg016840 vs. TAIR 10
Match: AT2G05260.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.7e-42
Identity = 110/365 (30.14%), Postives = 179/365 (49.04%), Query Frame = 0

Query: 60  FHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQAVYLLELDRQENRSNKENALAPK 119
           F++ GP ++++ +        W + +++R V AC +Q +Y++E DRQ  R   E ALA  
Sbjct: 8   FNLCGPTHLTTVD--------WGNEDHQRCVAACLVQGIYIVEQDRQLKREGTE-ALASP 67

Query: 120 WWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSP 179
           WW  F +KL++ L D+ D SIFG I E+   +   D+V    SG PR V+A RGTL K+ 
Sbjct: 68  WWESFNFKLIRHLKDDADFSIFGGIFEY--KSLQPDVV---DSGVPRYVIAFRGTLTKAD 127

Query: 180 TIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAESFGSNNVCIAGHSLGAGFALQVGK 239
           +I RDIE D+  +    L  + RF +A+QA++S+A+S G+++  + GHSLGA  AL  GK
Sbjct: 128 SITRDIELDIHIIR-NGLHRTSRFEIAMQAVRSMADSVGASSFWLTGHSLGAAMALLAGK 187

Query: 240 ALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWKRFKSMLPSGSETTENSNIEGEKA 299
            + K G+++++ LFNPP VS  +  R   E+     +   S++ +G   +       +  
Sbjct: 188 TMGKSGVYIKSLLFNPPYVSPPIE-RIANERVRHGIRFAGSLITAGLALSRTLKQTQQPQ 247

Query: 300 SAAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSDYICCSYTESDQVEKSNE------- 359
                L+      N  ++P   +  W+P+++VN  D++C  Y    +   + E       
Sbjct: 248 QQQLQLQ------NLTEDPLEALSSWLPNIHVNPGDHLCSEYIGFFEHRGNMEQIGYGAG 307

Query: 360 -------------------GKENINPTNGNGKAAAAAKLFVMSKGKQKFLEAHGLEQWWS 399
                              G  N            +A L V     + + +AHG+ QWW 
Sbjct: 308 IVERMAMQHSLGGLLMDAMGVSNAVEVEEPVHVIPSANLIVNKTISEDYKDAHGIHQWWR 350

BLAST of Spg016840 vs. TAIR 10
Match: AT2G05260.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 8.7e-41
Identity = 95/282 (33.69%), Postives = 157/282 (55.67%), Query Frame = 0

Query: 60  FHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQAVYLLELDRQENRSNKENALAPK 119
           F++ GP ++++ +        W + +++R V AC +Q +Y++E DRQ  R   E ALA  
Sbjct: 8   FNLCGPTHLTTVD--------WGNEDHQRCVAACLVQGIYIVEQDRQLKREGTE-ALASP 67

Query: 120 WWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSP 179
           WW  F +KL++ L D+ D SIFG I E+   +   D+V    SG PR V+A RGTL K+ 
Sbjct: 68  WWESFNFKLIRHLKDDADFSIFGGIFEY--KSLQPDVV---DSGVPRYVIAFRGTLTKAD 127

Query: 180 TIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAESFGSNNVCIAGHSLGAGFALQVGK 239
           +I RDIE D+  +    L  + RF +A+QA++S+A+S G+++  + GHSLGA  AL  GK
Sbjct: 128 SITRDIELDIHIIR-NGLHRTSRFEIAMQAVRSMADSVGASSFWLTGHSLGAAMALLAGK 187

Query: 240 ALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWKRFKSMLPSGSETTENSNIEGEKA 299
            + K G+++++ LFNPP VS  +  R   E+     +   S++ +G   +       +  
Sbjct: 188 TMGKSGVYIKSLLFNPPYVSPPIE-RIANERVRHGIRFAGSLITAGLALSRTLKQTQQPQ 247

Query: 300 SAAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSDYICCSY 342
                L+      N  ++P   +  W+P+++VN  D++C  Y
Sbjct: 248 QQQLQLQ------NLTEDPLEALSSWLPNIHVNPGDHLCSEY 267

BLAST of Spg016840 vs. TAIR 10
Match: AT5G24230.1 (Lipase class 3-related protein )

HSP 1 Score: 163.3 bits (412), Expect = 4.3e-40
Identity = 110/369 (29.81%), Postives = 178/369 (48.24%), Query Frame = 0

Query: 60  FHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQAVYLLELDRQENRSNKENALAPK 119
           F +SGP +++S +W    N+S+    ++ +V +C +QAVY LE DRQ+NR   ++  A  
Sbjct: 22  FSISGPIHLTSIDW----NNSY----HRTSVASCLVQAVYTLERDRQQNRIGLKSQ-ANH 81

Query: 120 WWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSP 179
           WW  F + L +TLID+ DGSI+GA+ E+    +            PR V+A RGT+LK  
Sbjct: 82  WWEFFNFTLAETLIDDSDGSIYGAVFEYKHFFSYNYHHTPHSKPPPRHVIAFRGTILKPH 141

Query: 180 TIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAESFGSNNVCIAGHSLGAGFALQVGK 239
           +  RD++ DLR +  +SL  S RF  A+Q ++S     G+  V +AGHSLGA  AL  GK
Sbjct: 142 SRSRDLKLDLRCIR-DSLHDSTRFVHAIQVIQSAVAKTGNAAVWLAGHSLGAAVALLAGK 201

Query: 240 ALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWKRFKSMLPSGSETTENSNIEGEKA 299
            + + G  +E++LFNPP  S+ +      EK +   +   S++               KA
Sbjct: 202 IMTRSGFPLESYLFNPPFSSIPIEKLVKSEKLKHGVRFAGSLV---------------KA 261

Query: 300 SAAAGLKSWVSNLNRLKNPGVGIGKWVPHLYVNNSDYICCSYT---------------ES 359
             A  +K    N  +  +  + +  W+P+LY+N  D IC  Y                + 
Sbjct: 262 GVAIAVKGRHHNKGQEDDSFMKLASWIPYLYLNPLDTICSEYIGYFKHRNKMFEIGAGKI 321

Query: 360 DQVEKSNEGKENINPTNGNGKAA----------AAAKLFVMSKGKQKFLEAHGLEQWWSD 404
           +++   N  +  ++   G G ++           +A + + +     F  AHG+ QWW  
Sbjct: 322 ERIATRNSLRSLLSGGGGGGSSSDSSSEPLHLLPSAYMTINASKSPNFKRAHGIHQWWDP 365

BLAST of Spg016840 vs. TAIR 10
Match: AT4G10955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 4.0e-38
Identity = 107/365 (29.32%), Postives = 174/365 (47.67%), Query Frame = 0

Query: 60  FHVSGPRNVSSPNWRDLINSSWKDGNYKRTVIACFIQAVYLLELDRQENRSNKENALAPK 119
           F ++GP +++S          W + +++R+V    +Q +Y+ E DRQ  R   E AL+P 
Sbjct: 9   FSLTGPLHLTS--------IDWANEHHRRSVAGSLVQGIYVAERDRQLQREGPELALSPI 68

Query: 120 WWIPFKYKLVQTLIDERDGSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSP 179
           W   F ++L++  +D+ D SIFG I E+     ++  V      +PR V+A RGT+ K  
Sbjct: 69  WSEFFHFRLIRKFVDDADNSIFGGIYEYKLPQQLSQTVK-SMEFSPRFVIAFRGTVTKVD 128

Query: 180 TIRRDIEDDLRFLAWESLKGSVRFNVALQALKSVAESFGSNNVCIAGHSLGAGFALQVGK 239
           +I RDIE D+  +    L  + RF +A+QA++++  S G ++V +AGHSLGA  AL  GK
Sbjct: 129 SISRDIEHDIHVIR-NGLHTTTRFEIAIQAVRNIVASVGGSSVWLAGHSLGASMALLTGK 188

Query: 240 ALAKEGIFVETHLFNPPSVSLAMSLRNIGEKAEFAWKRFKSMLPSGSETTENSNIEGEKA 299
            +A+ G F E   FNPP +S  +         +   KR K  +         S I    A
Sbjct: 189 TIARTGFFPECFAFNPPFLSAPIE--------KIKDKRIKHGI-----RIAGSVITAGLA 248

Query: 300 SAAAGLKSWVSNLNRL---KNPGVGIGKWVPHLYVNNSDYICCSYT----ESDQVEKSNE 359
            A    + +  N   L    +P   +  W P LYVN  D++C  Y       +++E+   
Sbjct: 249 LAKKATQHYSQNDRALPAPPDPFEALSDWFPRLYVNPGDHLCSEYVGYFEHRNKMEEIGI 308

Query: 360 G-KENINPTNGNG----------KAAAAAKLFVMSKGKQKFLEAHGLEQWWSDDLQLQLA 407
           G  E +   +  G              ++ L V     + F +AHG+ QWW +D + +  
Sbjct: 309 GFVERVATQHSLGGMLLGGQEPVHLIPSSVLTVNLSSSRDFKQAHGIHQWWREDNKFETK 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022936957.12.2e-20390.15GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata][more]
XP_038897095.11.4e-20291.23LOW QUALITY PROTEIN: GDSL esterase/lipase At4g10955 [Benincasa hispida][more]
KAG6591835.14.1e-20289.53GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023535014.17.0e-20289.70GDSL esterase/lipase At4g10955 [Cucurbita pepo subsp. pepo][more]
XP_022936956.11.2e-20190.08GDSL esterase/lipase At4g10955 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q680C05.7e-3729.32GDSL esterase/lipase At4g10955 OS=Arabidopsis thaliana OX=3702 GN=At4g10955 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1F9S81.1e-20390.15GDSL esterase/lipase At4g10955 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1F8X65.8e-20290.08GDSL esterase/lipase At4g10955 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IGP64.9e-20189.67GDSL esterase/lipase At4g10955 OS=Cucurbita maxima OX=3661 GN=LOC111477067 PE=4 ... [more]
A0A5D3CGI82.3e-19890.33GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold504... [more]
A0A1S3CQD42.3e-19890.33GDSL esterase/lipase At4g10955 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10350304... [more]
Match NameE-valueIdentityDescription
AT5G50890.11.1e-15270.65alpha/beta-Hydrolases superfamily protein [more]
AT2G05260.12.7e-4230.14alpha/beta-Hydrolases superfamily protein [more]
AT2G05260.28.7e-4133.69alpha/beta-Hydrolases superfamily protein [more]
AT5G24230.14.3e-4029.81Lipase class 3-related protein [more]
AT4G10955.14.0e-3829.32alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR31479:SF18GDSL ESTERASE/LIPASE PLANT-LIKE PROTEINcoord: 56..395
NoneNo IPR availablePANTHERPTHR31479ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 56..395
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 89..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016840.1Spg016840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane