Spg016837 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016837
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptiontransport inhibitor response 1-like protein
Locationscaffold9: 42191832 .. 42194512 (+)
RNA-Seq ExpressionSpg016837
SyntenySpg016837
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGATTCAAATAAAATAAATAAAAGAGAAATAGAGAGAGAGAGAGAGGCAAGAAGAATCCCAATGCCCAGGGATACAGACAGGAGAGCCTACTGTTTACACTCCATTGTCCTTGTGATCAGTTAAACTAATCCCTCCAAACTTCCATATCATTTCACAGTCCTCTCAAATCACAGGTATGGAGTTTCTCTCTCTTTCTCTCTTCATTTTCTCTCTCATCATCTTTTCCTAACCTAAATCCATTCTCTTTTGAAGCCTCCATGATCTGATTCAGTGTTGATTTGTTCTTTTTCTGCAGAAATCTCATTGAGGGGGGGTTTGTTTTTGACGAATTTGGGAGGTGTTGGAGTGTGATTTGTAGCTCATGAGAGTGGAGCAAGCTGAAGAAATGTCCGAAGACGACGATCGATCTTTACCTTCGGATCTAGGCGGCGACGCCATAGCTGAATCTGCAAGTAAAACTCGCAATTGCACTGGCTGTTCTGGCGATGTCGCCGGTTCTGGTTCTGCTGAGAATATTCTTCACAATGTTCTTGAGAACGTGCTTCACTTCCTCACTTCTCGCCGTGACCGGAACGCGGCTTCGCTTGTTTGTAAGTCGTGGTATCGCGTTGAAGCCCTCACTCGATCCGAACTGTTCATCGGGAATTGCTATGCTGTTTCCCCTCGCCGAGTCACGTCGAGGTTTAACCGAGTTCGGTCAGTGACGATCAAGGGAAGGCCGAGGTTCTCGGATTTCAACCTGATGCCGCCCAATTGGGGAGCTCACTTTACTCCTTGGGTTGCGGCCATGGCTAAATCCTATCCTTGGCTTGAGAGAATCTACTTGAAGCGTATGTCAGTCACTGACGATGATCTGGCGCTACTGGCCGAGTCTTTCCCTGGTTTCAAGGAGCTTGTGCTTTTTTGCTGCGAAGGGTTTGGTACCAGCGGGATTGCTGTGGTTGCTTCTAGGTGTAGGTACGCAAGCAGATTTCATCTTATCCTATTCATTTCTATCAATATCATGAGCTTGGAATTTTGTCACTCGTATTTGCATTACCTAATGTACATTTTGTATGTTTTTCGGGATCATTTCTTTGAGCAATGTGTTTTTTAATCTAAGCAAGACATATTGATGTTCTTGAAGGATACTGAACTGTGATAAGAAGATCTTAATAGGGGCAACTAATGCGGAACTTTTGTTTATGTTTATGAATTAATGGCTTGTTTTATTCAACTTAGGCTGCTTGATTTATCTTTCAGGCAGCTTCGAGTGCTTGATCTGATTGAATCTGACGTAGCAGATGATGAAGTGGATTGGATATCTTGTTTCCCAGAGAAGGAAACTTGTCTTGAATCTCTAGTTTTTGATTGCGTAGAATGTCCCATCAATTTTGAGGCACTGGAGATGTTGGTAAGTAGATCCCCGTTGTTGAAGAAACTTGGAGTGAATCGTTATGTTTCCATGGCACAGCTATACCACCTGATGGTTCGGGCTCCACAACTAACTCATCTTGGAACTGGTTCGTTCTGTAATCCAGAGGCCATGGTTCATGGGGAGCCAGAGCCTGACTATGCCGCTGCTTTTGCTGCTTGCAAATCCTTAGTCTGTCTCTCCGGATTCAAGGACATTTTGCCTGATTACCTGCCATGTATCTATCCAGTTTGCGCGAATCTCACCACTCTGAACTTGAGCTATGCTAATATAACTGCTGAACAACTCAAACCAGTTATACGCCACTGTCATAAACTCCGGACTTTCTGGGTATGCAATCTTTGAGTAGGTTCCTTATCAGGAAGGAATTTCGCTTGCAAAGCTTACAATTTTTTTTAAATCTTTATATTGCAGGCACTTGATTCAATATGCGATGAAGGACTTCAAGCTGTTGCTTCAACTTGTAAGGAACTGCAAGAACTTAGAGTTTTCCCTGTTGATGCTCGAGAAGATGGTGAAGGCCCAATTTCTGAAGTGGGCTTCCAAGCAATATCTGAGGGTTGCAGGAAATTGCAATATATCCTCTACTTCTGCCAGAGAATGACAAATGCAGCTGTGATTGCTATGTCAAAGAACTGCCAAGATCTTGTGGTGTTTAGGCTATGTATTATGGGGCGACACCAGCCAGACCATAAGACAGGGGAACCCATGGATGAAGGGTTTGGTGCCATTGTTATTAACTGTAAGAAGCTCACAAGGCTTGCTATATCTGGATTGTTGACTGATCGTGCTTTCAGCTATATTGGGAAGTATGGAAAACTGGTACGCACCCTATCAGTTGCTTTTGCTGGAAATAGTGATTTGGGATTGAAATATGTGCTCGAGGGTTGTCTTAGATTGCAAAAACTTGAGATTAGAGATAGCCCATTTGGTGATGTAGGCTTGCGATCTGGTTTACATCATTACTATAATATGAGATTCCTCTGGATGTCAGCTTGTAAATTGTCTCGCCAAGGCTGCCAAGAGGTTGCCAGAGCAATGCCTCACTTAGTGGTTGAGGTGATGAGGAATGAAAACGATGAGGAAATGGAAAACCATGTTCTGCCTCGCTTATTGGGTGAGGTGATGAGGAATGAAAACGATGAGGAAATGGAAAACCATATTCAGGTGGAGGGTATGGAGAACCATGTTCAGGTGTTATATATGTATAGATCTCTTGAGGGGCCGAGGGATGATGCTCCAAAGTTTGTTGACATCCTATAG

mRNA sequence

ATGAGAGTGGAGCAAGCTGAAGAAATGTCCGAAGACGACGATCGATCTTTACCTTCGGATCTAGGCGGCGACGCCATAGCTGAATCTGCAAGTAAAACTCGCAATTGCACTGGCTGTTCTGGCGATGTCGCCGGTTCTGGTTCTGCTGAGAATATTCTTCACAATGTTCTTGAGAACGTGCTTCACTTCCTCACTTCTCGCCGTGACCGGAACGCGGCTTCGCTTGTTTGTAAGTCGTGGTATCGCGTTGAAGCCCTCACTCGATCCGAACTGTTCATCGGGAATTGCTATGCTGTTTCCCCTCGCCGAGTCACGTCGAGGTTTAACCGAGTTCGGTCAGTGACGATCAAGGGAAGGCCGAGGTTCTCGGATTTCAACCTGATGCCGCCCAATTGGGGAGCTCACTTTACTCCTTGGGTTGCGGCCATGGCTAAATCCTATCCTTGGCTTGAGAGAATCTACTTGAAGCGTATGTCAGTCACTGACGATGATCTGGCGCTACTGGCCGAGTCTTTCCCTGGTTTCAAGGAGCTTGTGCTTTTTTGCTGCGAAGGGTTTGGTACCAGCGGGATTGCTGTGGTTGCTTCTAGGTGTAGGCTGCTTGATTTATCTTTCAGGCAGCTTCGAGTGCTTGATCTGATTGAATCTGACGTAGCAGATGATGAAGTGGATTGGATATCTTGTTTCCCAGAGAAGGAAACTTGTCTTGAATCTCTAGTTTTTGATTGCGTAGAATGTCCCATCAATTTTGAGGCACTGGAGATGTTGGTAAGTAGATCCCCGTTGTTGAAGAAACTTGGAGTGAATCGTTATGTTTCCATGGCACAGCTATACCACCTGATGGTTCGGGCTCCACAACTAACTCATCTTGGAACTGGTTCGTTCTGTAATCCAGAGGCCATGGTTCATGGGGAGCCAGAGCCTGACTATGCCGCTGCTTTTGCTGCTTGCAAATCCTTAGTCTGTCTCTCCGGATTCAAGGACATTTTGCCTGATTACCTGCCATGTATCTATCCAGTTTGCGCGAATCTCACCACTCTGAACTTGAGCTATGCTAATATAACTGCTGAACAACTCAAACCAGTTATACGCCACTGTCATAAACTCCGGACTTTCTGGGCACTTGATTCAATATGCGATGAAGGACTTCAAGCTGTTGCTTCAACTTGTAAGGAACTGCAAGAACTTAGAGTTTTCCCTGTTGATGCTCGAGAAGATGGTGAAGGCCCAATTTCTGAAGTGGGCTTCCAAGCAATATCTGAGGGTTGCAGGAAATTGCAATATATCCTCTACTTCTGCCAGAGAATGACAAATGCAGCTGTGATTGCTATGTCAAAGAACTGCCAAGATCTTGTGGTGTTTAGGCTATGTATTATGGGGCGACACCAGCCAGACCATAAGACAGGGGAACCCATGGATGAAGGGTTTGGTGCCATTGTTATTAACTGTAAGAAGCTCACAAGGCTTGCTATATCTGGATTGTTGACTGATCGTGCTTTCAGCTATATTGGGAAGTATGGAAAACTGGTACGCACCCTATCAGTTGCTTTTGCTGGAAATAGTGATTTGGGATTGAAATATGTGCTCGAGGGTTGTCTTAGATTGCAAAAACTTGAGATTAGAGATAGCCCATTTGGTGATGTAGGCTTGCGATCTGGTTTACATCATTACTATAATATGAGATTCCTCTGGATGTCAGCTTGTAAATTGTCTCGCCAAGGCTGCCAAGAGGTTGCCAGAGCAATGCCTCACTTAGTGGTTGAGGTGATGAGGAATGAAAACGATGAGGAAATGGAAAACCATGTTCTGCCTCGCTTATTGGGTGAGGTGATGAGGAATGAAAACGATGAGGAAATGGAAAACCATATTCAGGTGGAGGGTATGGAGAACCATGTTCAGGTGTTATATATGTATAGATCTCTTGAGGGGCCGAGGGATGATGCTCCAAAGTTTGTTGACATCCTATAG

Coding sequence (CDS)

ATGAGAGTGGAGCAAGCTGAAGAAATGTCCGAAGACGACGATCGATCTTTACCTTCGGATCTAGGCGGCGACGCCATAGCTGAATCTGCAAGTAAAACTCGCAATTGCACTGGCTGTTCTGGCGATGTCGCCGGTTCTGGTTCTGCTGAGAATATTCTTCACAATGTTCTTGAGAACGTGCTTCACTTCCTCACTTCTCGCCGTGACCGGAACGCGGCTTCGCTTGTTTGTAAGTCGTGGTATCGCGTTGAAGCCCTCACTCGATCCGAACTGTTCATCGGGAATTGCTATGCTGTTTCCCCTCGCCGAGTCACGTCGAGGTTTAACCGAGTTCGGTCAGTGACGATCAAGGGAAGGCCGAGGTTCTCGGATTTCAACCTGATGCCGCCCAATTGGGGAGCTCACTTTACTCCTTGGGTTGCGGCCATGGCTAAATCCTATCCTTGGCTTGAGAGAATCTACTTGAAGCGTATGTCAGTCACTGACGATGATCTGGCGCTACTGGCCGAGTCTTTCCCTGGTTTCAAGGAGCTTGTGCTTTTTTGCTGCGAAGGGTTTGGTACCAGCGGGATTGCTGTGGTTGCTTCTAGGTGTAGGCTGCTTGATTTATCTTTCAGGCAGCTTCGAGTGCTTGATCTGATTGAATCTGACGTAGCAGATGATGAAGTGGATTGGATATCTTGTTTCCCAGAGAAGGAAACTTGTCTTGAATCTCTAGTTTTTGATTGCGTAGAATGTCCCATCAATTTTGAGGCACTGGAGATGTTGGTAAGTAGATCCCCGTTGTTGAAGAAACTTGGAGTGAATCGTTATGTTTCCATGGCACAGCTATACCACCTGATGGTTCGGGCTCCACAACTAACTCATCTTGGAACTGGTTCGTTCTGTAATCCAGAGGCCATGGTTCATGGGGAGCCAGAGCCTGACTATGCCGCTGCTTTTGCTGCTTGCAAATCCTTAGTCTGTCTCTCCGGATTCAAGGACATTTTGCCTGATTACCTGCCATGTATCTATCCAGTTTGCGCGAATCTCACCACTCTGAACTTGAGCTATGCTAATATAACTGCTGAACAACTCAAACCAGTTATACGCCACTGTCATAAACTCCGGACTTTCTGGGCACTTGATTCAATATGCGATGAAGGACTTCAAGCTGTTGCTTCAACTTGTAAGGAACTGCAAGAACTTAGAGTTTTCCCTGTTGATGCTCGAGAAGATGGTGAAGGCCCAATTTCTGAAGTGGGCTTCCAAGCAATATCTGAGGGTTGCAGGAAATTGCAATATATCCTCTACTTCTGCCAGAGAATGACAAATGCAGCTGTGATTGCTATGTCAAAGAACTGCCAAGATCTTGTGGTGTTTAGGCTATGTATTATGGGGCGACACCAGCCAGACCATAAGACAGGGGAACCCATGGATGAAGGGTTTGGTGCCATTGTTATTAACTGTAAGAAGCTCACAAGGCTTGCTATATCTGGATTGTTGACTGATCGTGCTTTCAGCTATATTGGGAAGTATGGAAAACTGGTACGCACCCTATCAGTTGCTTTTGCTGGAAATAGTGATTTGGGATTGAAATATGTGCTCGAGGGTTGTCTTAGATTGCAAAAACTTGAGATTAGAGATAGCCCATTTGGTGATGTAGGCTTGCGATCTGGTTTACATCATTACTATAATATGAGATTCCTCTGGATGTCAGCTTGTAAATTGTCTCGCCAAGGCTGCCAAGAGGTTGCCAGAGCAATGCCTCACTTAGTGGTTGAGGTGATGAGGAATGAAAACGATGAGGAAATGGAAAACCATGTTCTGCCTCGCTTATTGGGTGAGGTGATGAGGAATGAAAACGATGAGGAAATGGAAAACCATATTCAGGTGGAGGGTATGGAGAACCATGTTCAGGTGTTATATATGTATAGATCTCTTGAGGGGCCGAGGGATGATGCTCCAAAGTTTGTTGACATCCTATAG

Protein sequence

MRVEQAEEMSEDDDRSLPSDLGGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL
Homology
BLAST of Spg016837 vs. NCBI nr
Match: XP_023524117.1 (transport inhibitor response 1-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 595/660 (90.15%), Postives = 610/660 (92.42%), Query Frame = 0

Query: 1   MRVEQAEEMSEDDDRSLPSDL-----GGDAIAESASKTRNCTGCSGDVAGSGSAENILHN 60
           MRVEQAEEMSEDDDRSLP DL     GG A+AESASKTRNCTGC GD+A SGS EN LHN
Sbjct: 1   MRVEQAEEMSEDDDRSLPLDLGGGGGGGGAVAESASKTRNCTGCYGDIAASGSVENTLHN 60

Query: 61  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 120
           VLENVLHFLTSR DRNAASLVCKSW  VEALTRSELFIGNCYAVSPRRVTSRF RVRSVT
Sbjct: 61  VLENVLHFLTSRHDRNAASLVCKSWCGVEALTRSELFIGNCYAVSPRRVTSRFKRVRSVT 120

Query: 121 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 180
           IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF
Sbjct: 121 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 180

Query: 181 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 240
           KELVLFCCEGFGTSGIAVVASRC       RQLRVLDLIESD+ADDEVDWISCFPE ETC
Sbjct: 181 KELVLFCCEGFGTSGIAVVASRC-------RQLRVLDLIESDIADDEVDWISCFPENETC 240

Query: 241 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 300
           LESLVFDCVECPINFEALE LVSRSPLLKKLGVNRYVS+AQLYHL+VRAPQLTHLGTGSF
Sbjct: 241 LESLVFDCVECPINFEALETLVSRSPLLKKLGVNRYVSIAQLYHLIVRAPQLTHLGTGSF 300

Query: 301 CNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANIT 360
           CNPEAM+HGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYP CANLTTLNLSYANI+
Sbjct: 301 CNPEAMIHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPACANLTTLNLSYANIS 360

Query: 361 AEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVG 420
           AEQLKPVI HCHKL+TFWALDSICDEGLQAVASTCK+LQELRVFPVD REDGEGPISEVG
Sbjct: 361 AEQLKPVISHCHKLQTFWALDSICDEGLQAVASTCKDLQELRVFPVDPREDGEGPISEVG 420

Query: 421 FQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEG 480
           FQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEG
Sbjct: 421 FQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEG 480

Query: 481 FGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRL 540
           FGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAG+SDLGLK+VLEGCLRL
Sbjct: 481 FGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGDSDLGLKFVLEGCLRL 540

Query: 541 QKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNEND 600
           QKLEIRDSPFG+ GL+SGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV        
Sbjct: 541 QKLEIRDSPFGNGGLQSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV-------- 600

Query: 601 EEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
                        EVMRNENDEE++NHIQVE MENHVQVLYMYRSLEGPRDDAPKFVDIL
Sbjct: 601 -------------EVMRNENDEEVDNHIQVEDMENHVQVLYMYRSLEGPRDDAPKFVDIL 632

BLAST of Spg016837 vs. NCBI nr
Match: XP_022941095.1 (transport inhibitor response 1-like protein [Cucurbita moschata] >XP_022941097.1 transport inhibitor response 1-like protein [Cucurbita moschata] >XP_022941098.1 transport inhibitor response 1-like protein [Cucurbita moschata])

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 589/649 (90.76%), Postives = 604/649 (93.07%), Query Frame = 0

Query: 9   MSEDDDRSLPSDL--GGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFLTS 68
           MSEDDDRSLP DL  GG A+AESASKTRNCTGC G +A SGS EN LHNVLENVLHFLTS
Sbjct: 1   MSEDDDRSLPLDLGGGGGAVAESASKTRNCTGCYGHLAASGSVENTLHNVLENVLHFLTS 60

Query: 69  RRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFN 128
           R DRNAASLVCKSWY VEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFN
Sbjct: 61  RHDRNAASLVCKSWYGVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFN 120

Query: 129 LMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGF 188
           LMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGF
Sbjct: 121 LMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGF 180

Query: 189 GTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCVEC 248
           GTSGIAVVASRC       RQLRVLDLIESD+ADDEVDWISCFPE ETCLESLVFDCVEC
Sbjct: 181 GTSGIAVVASRC-------RQLRVLDLIESDIADDEVDWISCFPENETCLESLVFDCVEC 240

Query: 249 PINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHGEP 308
           PINFEALEMLVSRSPLLKKLGVNRYVS+AQLYHL+VRAPQLTHLGTGSFCNPEAM+HGEP
Sbjct: 241 PINFEALEMLVSRSPLLKKLGVNRYVSIAQLYHLIVRAPQLTHLGTGSFCNPEAMIHGEP 300

Query: 309 EPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIRHC 368
           EPDYAAAFAACKSLVCLSGFKDILPDYLPCIYP CANLTTLNLSYANI+AEQLKPVI HC
Sbjct: 301 EPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPACANLTTLNLSYANISAEQLKPVISHC 360

Query: 369 HKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKL 428
           HKL+TFWALDSICDEGLQAVASTCK+LQELRVFPVD REDGEGPISEVGFQAISEGCRKL
Sbjct: 361 HKLQTFWALDSICDEGLQAVASTCKDLQELRVFPVDPREDGEGPISEVGFQAISEGCRKL 420

Query: 429 QYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKL 488
           QYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKL
Sbjct: 421 QYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKL 480

Query: 489 TRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFG 548
           TRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAG+SDLGLK+VLEGCLRLQKLEIRDSPFG
Sbjct: 481 TRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGDSDLGLKFVLEGCLRLQKLEIRDSPFG 540

Query: 549 DVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLPRL 608
           + GL+SGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV                   
Sbjct: 541 NGGLQSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV------------------- 600

Query: 609 LGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
             EVMRNENDEE++NHIQVE MENHVQVLYMYRSLEGPRDDAPKFVDIL
Sbjct: 601 --EVMRNENDEEVDNHIQVEDMENHVQVLYMYRSLEGPRDDAPKFVDIL 621

BLAST of Spg016837 vs. NCBI nr
Match: XP_022982471.1 (transport inhibitor response 1-like protein isoform X3 [Cucurbita maxima] >XP_022982472.1 transport inhibitor response 1-like protein isoform X3 [Cucurbita maxima])

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 590/648 (91.05%), Postives = 605/648 (93.36%), Query Frame = 0

Query: 9   MSEDDDRSLPSDL-GGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFLTSR 68
           MSEDDDRSLP DL GG A+AESASKTRNCTGC GD+A SGSAEN LHNVLENVLHFLTSR
Sbjct: 1   MSEDDDRSLPLDLGGGGAVAESASKTRNCTGCYGDLAASGSAENTLHNVLENVLHFLTSR 60

Query: 69  RDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFNL 128
            DRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRF RVRSVTIKGRPRFSDFNL
Sbjct: 61  HDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFKRVRSVTIKGRPRFSDFNL 120

Query: 129 MPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFG 188
           MPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFG
Sbjct: 121 MPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFG 180

Query: 189 TSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCVECP 248
           TSGIAVVASRC       RQLRVLDLIESD+ADDEVDWISCFPEKET LESLVFDCVECP
Sbjct: 181 TSGIAVVASRC-------RQLRVLDLIESDIADDEVDWISCFPEKETYLESLVFDCVECP 240

Query: 249 INFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHGEPE 308
           INFEALEMLVSRSPLLKKLGVNRYVS+AQLYHL+VRAPQLTHLGTGSFCNPEAM+HGEPE
Sbjct: 241 INFEALEMLVSRSPLLKKLGVNRYVSIAQLYHLIVRAPQLTHLGTGSFCNPEAMIHGEPE 300

Query: 309 PDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIRHCH 368
           PDYAAAFAACKSLVCLSGFKDILPDYLP IYP CANLTTLNLSYANI+AEQLKPVI HCH
Sbjct: 301 PDYAAAFAACKSLVCLSGFKDILPDYLPFIYPACANLTTLNLSYANISAEQLKPVISHCH 360

Query: 369 KLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQ 428
           KL+TFWALDSICDEGLQAVASTCK+LQELRVFPVD REDGEGPISEVGFQAISEGCRKLQ
Sbjct: 361 KLQTFWALDSICDEGLQAVASTCKDLQELRVFPVDPREDGEGPISEVGFQAISEGCRKLQ 420

Query: 429 YILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLT 488
           YILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLT
Sbjct: 421 YILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLT 480

Query: 489 RLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGD 548
           RLAISGLLTDRAFSYIGKYGKLVRTLSVAFAG+SDLGLK+VLEGCLRLQKLEIRDSPFG+
Sbjct: 481 RLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGDSDLGLKFVLEGCLRLQKLEIRDSPFGN 540

Query: 549 VGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLPRLL 608
            GL+SGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV                    
Sbjct: 541 GGLQSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV-------------------- 600

Query: 609 GEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
            EVMRNENDEE++NHIQVE MENHVQVLYMYRSLEGPRDDAPKFVDIL
Sbjct: 601 -EVMRNENDEEVDNHIQVEDMENHVQVLYMYRSLEGPRDDAPKFVDIL 620

BLAST of Spg016837 vs. NCBI nr
Match: KAG7037613.1 (Transport inhibitor response 1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 588/651 (90.32%), Postives = 604/651 (92.78%), Query Frame = 0

Query: 9   MSEDDDRSLPSDL----GGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFL 68
           MSEDDDRSLP DL    GG A+AESASKTRNCTGC G +A SGS EN LHNVLENVLHFL
Sbjct: 1   MSEDDDRSLPLDLGGGGGGGAVAESASKTRNCTGCYGHLAASGSVENTLHNVLENVLHFL 60

Query: 69  TSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSD 128
           TSR DRNAASLVCKSWY VEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSD
Sbjct: 61  TSRHDRNAASLVCKSWYGVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSD 120

Query: 129 FNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCE 188
           FNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCE
Sbjct: 121 FNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCE 180

Query: 189 GFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCV 248
           GFGTSGIAVVASRC       RQLRVLDLIESD+ADDEVDWISCFPE ETCLESLVFDCV
Sbjct: 181 GFGTSGIAVVASRC-------RQLRVLDLIESDIADDEVDWISCFPENETCLESLVFDCV 240

Query: 249 ECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHG 308
           ECPINFEALEMLVSRSPLLKKLGVNRYVS+AQLYHL+VRAPQLTHLGTGSFCNPEAM+HG
Sbjct: 241 ECPINFEALEMLVSRSPLLKKLGVNRYVSIAQLYHLIVRAPQLTHLGTGSFCNPEAMIHG 300

Query: 309 EPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIR 368
           EPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYP CANLTTLNLSYANI+AEQLKPVI 
Sbjct: 301 EPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPACANLTTLNLSYANISAEQLKPVIS 360

Query: 369 HCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCR 428
           HCHKL+TFWALDSICDEGLQAVASTCK+LQELRVFPVD REDGEGPISEVGFQAISEGCR
Sbjct: 361 HCHKLQTFWALDSICDEGLQAVASTCKDLQELRVFPVDPREDGEGPISEVGFQAISEGCR 420

Query: 429 KLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCK 488
           KLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCK
Sbjct: 421 KLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCK 480

Query: 489 KLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSP 548
           KLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAG+SDLGLK+VLEGCL+LQKLEIRDSP
Sbjct: 481 KLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGDSDLGLKFVLEGCLKLQKLEIRDSP 540

Query: 549 FGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLP 608
           FG+ GL+SGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV                 
Sbjct: 541 FGNGGLQSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV----------------- 600

Query: 609 RLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
               EVMRNENDEE++NHIQVE MENHVQVLYMYRSLEGPRDDAPKFVDIL
Sbjct: 601 ----EVMRNENDEEVDNHIQVEDMENHVQVLYMYRSLEGPRDDAPKFVDIL 623

BLAST of Spg016837 vs. NCBI nr
Match: XP_022136070.1 (transport inhibitor response 1-like protein [Momordica charantia])

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 584/665 (87.82%), Postives = 610/665 (91.73%), Query Frame = 0

Query: 9   MSEDDDRSLPSDLGGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFLTSRR 68
           MSEDDDRSL SDLG    A+SASK RNCTGCSGDV+GSGSAENILHNVLENVLHFLTSRR
Sbjct: 1   MSEDDDRSLTSDLGA---AQSASKARNCTGCSGDVSGSGSAENILHNVLENVLHFLTSRR 60

Query: 69  DRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFNLM 128
           DR+AASLVCKSWYRVEALTRS+LFIGNCYAVSP RVT+RF RVRSV+IKG+PRF+DFNLM
Sbjct: 61  DRSAASLVCKSWYRVEALTRSDLFIGNCYAVSPCRVTARFGRVRSVSIKGKPRFADFNLM 120

Query: 129 PPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFGT 188
           PPNWGAHF PWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFGT
Sbjct: 121 PPNWGAHFAPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFGT 180

Query: 189 SGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCVECPI 248
           SGIAVVASRC       R LRVLDLIESDV DDEVDWISCFPEKETCLESLVFDCVECPI
Sbjct: 181 SGIAVVASRC-------RHLRVLDLIESDVTDDEVDWISCFPEKETCLESLVFDCVECPI 240

Query: 249 NFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHGEPEP 308
           NFEALEMLV+RSP LKKLGVNRY+S+ QLYHLM+RAP+LTHLGTGSFCNPEA+VHGE EP
Sbjct: 241 NFEALEMLVTRSPYLKKLGVNRYISITQLYHLMIRAPKLTHLGTGSFCNPEAIVHGELEP 300

Query: 309 DYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIRHCHK 368
           DYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVI HCHK
Sbjct: 301 DYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVISHCHK 360

Query: 369 LRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQY 428
           L+TFWALDSICD+GLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQY
Sbjct: 361 LQTFWALDSICDDGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQY 420

Query: 429 ILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLTR 488
           ILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLTR
Sbjct: 421 ILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLTR 480

Query: 489 LAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGDV 548
           LAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGD 
Sbjct: 481 LAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGDG 540

Query: 549 GLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLPRLLG 608
            LRSGLHHYYNMRFLWMS+CKLSRQGCQEVARAMPHLVVEVMRNEN E++ NH+    + 
Sbjct: 541 ALRSGLHHYYNMRFLWMSSCKLSRQGCQEVARAMPHLVVEVMRNENGEDINNHIQVEDME 600

Query: 609 EVMRNENDEE------------------MENHIQVEGMENHVQVLYMYRSLEGPRDDAPK 656
             ++ E+ EE                  +ENH+QVE MENHVQVLYMYRSLEGPRDDAP 
Sbjct: 601 NYVQVEDMEENHVQVEEDMEENHVQVEDVENHVQVEDMENHVQVLYMYRSLEGPRDDAPN 655

BLAST of Spg016837 vs. ExPASy Swiss-Prot
Match: Q9LTX2 (Transport inhibitor response 1-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g49980 PE=2 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 2.2e-258
Identity = 439/660 (66.52%), Postives = 530/660 (80.30%), Query Frame = 0

Query: 4   EQAEEMSEDDD--RSLPSDLGGDAIAE------SASKTRNCTGCSGDVAGSGSAENILHN 63
           +   EMSEDDD  +S P DL   AIA+      S +K+RNC   S         +++L N
Sbjct: 3   QDRSEMSEDDDDQQSPPLDLPSTAIADPCSSSSSPNKSRNCISNS-----QTFPDHVLEN 62

Query: 64  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 123
           VLENVL FL SR DRNAASLVCKSW+RVEALTRSE+FIGNCYA+SP R+T RF RVRS+ 
Sbjct: 63  VLENVLQFLDSRCDRNAASLVCKSWWRVEALTRSEVFIGNCYALSPARLTQRFKRVRSLV 122

Query: 124 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 183
           +KG+PRF+DFNLMPP+WGA+F PWV+ MA++YP LE++ LKRM VTDDDLALLA+SFPGF
Sbjct: 123 LKGKPRFADFNLMPPDWGANFAPWVSTMAQAYPCLEKVDLKRMFVTDDDLALLADSFPGF 182

Query: 184 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 243
           KEL+L CCEGFGTSGI++VA++C       R+L+VLDLIES+V DDEVDWISCFPE  TC
Sbjct: 183 KELILVCCEGFGTSGISIVANKC-------RKLKVLDLIESEVTDDEVDWISCFPEDVTC 242

Query: 244 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 303
           LESL FDCVE PINF+ALE LV+RSP LKKL +NR+VS+ +L+ L++ APQLT LGTGSF
Sbjct: 243 LESLAFDCVEAPINFKALEGLVARSPFLKKLRLNRFVSLVELHRLLLGAPQLTSLGTGSF 302

Query: 304 CNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANIT 363
            + E     E EPDYAAAF ACKS+VCLSGF++++P+YLP I+PVCANLT+LN SYANI+
Sbjct: 303 SHDEE-PQSEQEPDYAAAFRACKSVVCLSGFRELMPEYLPAIFPVCANLTSLNFSYANIS 362

Query: 364 AEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVG 423
            +  KP+I +CHKL+ FWALDSICDEGLQAVA+TCKEL+ELR+FP D RED EGP+SE+G
Sbjct: 363 PDMFKPIILNCHKLQVFWALDSICDEGLQAVAATCKELRELRIFPFDPREDSEGPVSELG 422

Query: 424 FQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEG 483
            QAISEGCRKL+ ILYFCQRMTNAAVIAMS+NC +L VFRLCIMGRH+PDH TG+PMDEG
Sbjct: 423 LQAISEGCRKLESILYFCQRMTNAAVIAMSENCPELTVFRLCIMGRHRPDHVTGKPMDEG 482

Query: 484 FGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRL 543
           FGAIV NCKKLTRLA+SGLLTD+AF Y+G+YGKLVRTLSVAFAG+SD+ L++VLEGC RL
Sbjct: 483 FGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLVRTLSVAFAGDSDMALRHVLEGCPRL 542

Query: 544 QKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNEND 603
           QKLEIRDSPFGDV LRSG+H YYNMRF+WMSAC LS+  C+++ARAMP+LVVEV+ +++D
Sbjct: 543 QKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACSLSKGCCKDIARAMPNLVVEVIGSDDD 602

Query: 604 EEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
           ++                                ++V+ LYMYRSL+GPR+DAPKFV IL
Sbjct: 603 DD------------------------------NRDYVETLYMYRSLDGPRNDAPKFVTIL 619

BLAST of Spg016837 vs. ExPASy Swiss-Prot
Match: Q8RWQ8 (F-box protein FBX14 OS=Arabidopsis thaliana OX=3702 GN=FBX14 PE=1 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 8.7e-247
Identity = 425/658 (64.59%), Postives = 509/658 (77.36%), Query Frame = 0

Query: 4   EQAEEMSEDDDRSL------PSDLGGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVL 63
           + + +MSED ++ L       S     + A   +K+RN        +     +++L NVL
Sbjct: 5   DSSAKMSEDVEKYLNLNPPCSSSSSSSSAATFTNKSRNFKS-----SPPPCPDHVLENVL 64

Query: 64  ENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIK 123
           ENVL FLTSR DRNA SLVC+SWYRVEA TR E+FIGNCY++SP R+  RF RVRS+ +K
Sbjct: 65  ENVLQFLTSRCDRNAVSLVCRSWYRVEAQTRLEVFIGNCYSLSPARLIHRFKRVRSLVLK 124

Query: 124 GRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKE 183
           G+PRF+DFNLMPPNWGA F+PWVAA AK+YPWLE+++LKRM VTDDDLALLAESFPGFKE
Sbjct: 125 GKPRFADFNLMPPNWGAQFSPWVAATAKAYPWLEKVHLKRMFVTDDDLALLAESFPGFKE 184

Query: 184 LVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLE 243
           L L CCEGFGTSGIA+VA++C       RQL+VLDL+ES+V DDE+DWISCFPE ET LE
Sbjct: 185 LTLVCCEGFGTSGIAIVANKC-------RQLKVLDLMESEVTDDELDWISCFPEGETHLE 244

Query: 244 SLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCN 303
           SL FDCVE PINF+ALE LV RSP LKKL  NR+VS+ +L+ LMVRAPQLT LGTGSF +
Sbjct: 245 SLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLTSLGTGSF-S 304

Query: 304 PEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAE 363
           P+ +  GE +PDYAAAF ACKS+VCLSGF++  P+YL  I  VCANLT+LN SYANI+  
Sbjct: 305 PDNVPQGEQQPDYAAAFRACKSIVCLSGFREFRPEYLLAISSVCANLTSLNFSYANISPH 364

Query: 364 QLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQ 423
            LKP+I +CH +R FWALDSI DEGLQAVA+TCKEL+ELR+FP D RED EGP+S VG Q
Sbjct: 365 MLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELRELRIFPFDPREDSEGPVSGVGLQ 424

Query: 424 AISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFG 483
           AISEGCRKL+ ILYFCQ MTN AV AMS+NC  L VFRLCIMGRH+PDH TG+PMD+GFG
Sbjct: 425 AISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVFRLCIMGRHRPDHVTGKPMDDGFG 484

Query: 484 AIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQK 543
           AIV NCKKLTRLA+SGLLTD AFSYIG+YGKL+RTLSVAFAGNSD  L+YVLEGC +LQK
Sbjct: 485 AIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLSVAFAGNSDKALRYVLEGCPKLQK 544

Query: 544 LEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEE 603
           LEIRDSPFGDVGLRSG+H Y NMRF+W+S+C +SR GC+ V+ A+P++VVEV   + D++
Sbjct: 545 LEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGGCRGVSHALPNVVVEVFGADGDDD 604

Query: 604 MENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
            +                         V G  ++V+ LY+YRSL+GPR DAPKFV IL
Sbjct: 605 EDT------------------------VTG--DYVETLYLYRSLDGPRKDAPKFVTIL 623

BLAST of Spg016837 vs. ExPASy Swiss-Prot
Match: Q9LW29 (Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.8e-159
Identity = 296/601 (49.25%), Postives = 384/601 (63.89%), Query Frame = 0

Query: 56  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 115
           V+E+V  F+TS +DRNA SLVCKSWY++E  +R ++FIGNCYA++P R+  RF  ++S+T
Sbjct: 8   VIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCLKSLT 67

Query: 116 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 175
           +KG+P F+DFNL+P  WG    PW+ A+A+S   LE + LKRM VTD+ L LL+ SF  F
Sbjct: 68  LKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRSFVNF 127

Query: 176 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 235
           K LVL  CEGF T G+A +A+ C       R LR LDL E+++ D    W+SCFP+  T 
Sbjct: 128 KSLVLVSCEGFTTDGLASIAANC-------RHLRDLDLQENEIDDHRGQWLSCFPDTCTT 187

Query: 236 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 295
           L +L F C+E   N  ALE LV+RSP LK L +NR V +  L  LM  APQ+  LG GS+
Sbjct: 188 LVTLNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSY 247

Query: 296 CNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSY-ANI 355
            N       E      A    C SL  LSGF +  P  L   +P+C NLT+LNLSY A I
Sbjct: 248 ENDP---DSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEI 307

Query: 356 TAEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEV 415
               L  +I+HC KL+  W LDSI D+GL+ VASTCKELQELRVFP D    G   ++E 
Sbjct: 308 HGSHLIKLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNTAVTEE 367

Query: 416 GFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDE 475
           G  AIS GC KL  ILYFCQ+MTNAA++ ++KNC + + FRLCI+  ++PDH T +P+DE
Sbjct: 368 GLVAISAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDE 427

Query: 476 GFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLR 535
           GFGAIV  CK L RL++SGLLTD+ F YIG Y   +  LS+AFAG++D G+ YVL GC +
Sbjct: 428 GFGAIVKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKK 487

Query: 536 LQKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNEN 595
           ++KLEIRDSPFGD  L + +  Y  MR LWMS+C+++  GC+ +A   P L VE++ NEN
Sbjct: 488 MKKLEIRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEII-NEN 547

Query: 596 DEEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDI 655
           D                   N  E   H   EG +  V  LY+YR++ G R DAP FV I
Sbjct: 548 D------------------NNRMEENGH---EGRQK-VDKLYLYRTVVGTRMDAPPFVWI 575

BLAST of Spg016837 vs. ExPASy Swiss-Prot
Match: Q7XVM8 (Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0395600 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 9.8e-158
Identity = 296/601 (49.25%), Postives = 382/601 (63.56%), Query Frame = 0

Query: 56  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 115
           V+E++  FL ++RDRN  SLVCK WY +E L+R  +F+GNCYAV   RV +RF  VR++T
Sbjct: 8   VVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNVRALT 67

Query: 116 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 175
           +KG+P F+DFNL+PP+WG +  PW+ A A+    LE + +KRM V+D+ L LLA SFP F
Sbjct: 68  VKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARSFPRF 127

Query: 176 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 235
           + LVL  CEGF T G+A VAS C+L       LR LDL E++V D    W+SCFP+  T 
Sbjct: 128 RALVLISCEGFSTDGLAAVASHCKL-------LRELDLQENEVEDRGPRWLSCFPDSCTS 187

Query: 236 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 295
           L SL F C++  +N  +LE LVSRSP L+ L +NR VS+  L  +++R P L  LGTG+ 
Sbjct: 188 LVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNL 247

Query: 296 CNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYA-NI 355
            +       E      +A   CK L  LSGF D  P  L  IYP+CA LT LNLSYA  +
Sbjct: 248 TDD---FQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTL 307

Query: 356 TAEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEV 415
            A  L  +I  C KL+  W LD I D+GLQ VAS+CK+LQELRVFP D    G   ++E 
Sbjct: 308 DASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEE 367

Query: 416 GFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDE 475
           G  A+S GC KL  +LYFC +MTNAA++ ++KNC +   FRLCI+   +PD  T +P+DE
Sbjct: 368 GLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDE 427

Query: 476 GFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLR 535
           GFGAIV  CK L RL+ISGLLTD+ F YIGKY K +  LS+AFAG+SD G+ +V+ GC  
Sbjct: 428 GFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKN 487

Query: 536 LQKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNEN 595
           L+KLEIRDSPFGD  L      Y  MR LWMS+C ++ +GCQ +A  MP L VEV+ NE 
Sbjct: 488 LRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVI-NE- 547

Query: 596 DEEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDI 655
                            R+ ++E  ENH    G    V+ LY+YR+  G RDDAP FV I
Sbjct: 548 -----------------RDGSNEMEENH----GDLPKVEKLYVYRTTAGARDDAPNFVKI 575

BLAST of Spg016837 vs. ExPASy Swiss-Prot
Match: Q9LPW7 (Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 2.2e-157
Identity = 293/607 (48.27%), Postives = 390/607 (64.25%), Query Frame = 0

Query: 56  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 115
           V+E+V  F+ S +DRN+ SLVCKSW+++E  +R E+FIGNCYA++P R+  RF  ++S+T
Sbjct: 8   VIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRFPCLKSLT 67

Query: 116 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 175
           +KG+P F+DFNL+P  WG    PW+ A+A+S   LE + LKRM VTD+ L LL+ SF  F
Sbjct: 68  LKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLLSRSFANF 127

Query: 176 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 235
           K LVL  CEGF T G+A +A+ C       R LR LDL E+++ D    W++CFP+  T 
Sbjct: 128 KSLVLVSCEGFTTDGLASIAANC-------RHLRELDLQENEIDDHRGQWLNCFPDSCTT 187

Query: 236 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 295
           L SL F C++   N  ALE LV+RSP LK L +NR V +  L  LM  APQL  LG GS+
Sbjct: 188 LMSLNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSY 247

Query: 296 CNPEAMVHGEPEPD-YAAAFAACK---SLVCLSGFKDILPDYLPCIYPVCANLTTLNLSY 355
            N       EP+P+ +A    A K   SL  LSGF ++ P  LP  YP+C NL +LNLSY
Sbjct: 248 EN-------EPDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSY 307

Query: 356 -ANITAEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVD--AREDGE 415
            A I    L  +I+ C +L+  W LDSI D+GL  VA+TCKELQELRVFP D    ED  
Sbjct: 308 AAEIQGNHLIKLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSDVHGEEDNN 367

Query: 416 GPISEVGFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKT 475
             ++EVG  AIS GC KL  ILYFC++MTNAA+IA++KNC + + FRLCI+  H+PDH T
Sbjct: 368 ASVTEVGLVAISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHIT 427

Query: 476 GEPMDEGFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYV 535
            + +DEGFGAIV  CK L RL++SGLLTD+ F YIG Y + +  LS+AFAG++D G+ YV
Sbjct: 428 FQSLDEGFGAIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYV 487

Query: 536 LEGCLRLQKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVE 595
           L GC +++KLEIRDSPFG+  L + +  Y  MR LWMS+C+++  GC+ +A+  P L VE
Sbjct: 488 LNGCKKMRKLEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVE 547

Query: 596 VMRNENDEEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDA 655
           ++    +  ME            +NE DE              V  LY+YR++ G R DA
Sbjct: 548 IINENENNGME------------QNEEDE-----------REKVDKLYLYRTVVGTRKDA 577

BLAST of Spg016837 vs. ExPASy TrEMBL
Match: A0A6J1FR48 (transport inhibitor response 1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111446495 PE=4 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 589/649 (90.76%), Postives = 604/649 (93.07%), Query Frame = 0

Query: 9   MSEDDDRSLPSDL--GGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFLTS 68
           MSEDDDRSLP DL  GG A+AESASKTRNCTGC G +A SGS EN LHNVLENVLHFLTS
Sbjct: 1   MSEDDDRSLPLDLGGGGGAVAESASKTRNCTGCYGHLAASGSVENTLHNVLENVLHFLTS 60

Query: 69  RRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFN 128
           R DRNAASLVCKSWY VEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFN
Sbjct: 61  RHDRNAASLVCKSWYGVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFN 120

Query: 129 LMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGF 188
           LMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGF
Sbjct: 121 LMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGF 180

Query: 189 GTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCVEC 248
           GTSGIAVVASRC       RQLRVLDLIESD+ADDEVDWISCFPE ETCLESLVFDCVEC
Sbjct: 181 GTSGIAVVASRC-------RQLRVLDLIESDIADDEVDWISCFPENETCLESLVFDCVEC 240

Query: 249 PINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHGEP 308
           PINFEALEMLVSRSPLLKKLGVNRYVS+AQLYHL+VRAPQLTHLGTGSFCNPEAM+HGEP
Sbjct: 241 PINFEALEMLVSRSPLLKKLGVNRYVSIAQLYHLIVRAPQLTHLGTGSFCNPEAMIHGEP 300

Query: 309 EPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIRHC 368
           EPDYAAAFAACKSLVCLSGFKDILPDYLPCIYP CANLTTLNLSYANI+AEQLKPVI HC
Sbjct: 301 EPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPACANLTTLNLSYANISAEQLKPVISHC 360

Query: 369 HKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKL 428
           HKL+TFWALDSICDEGLQAVASTCK+LQELRVFPVD REDGEGPISEVGFQAISEGCRKL
Sbjct: 361 HKLQTFWALDSICDEGLQAVASTCKDLQELRVFPVDPREDGEGPISEVGFQAISEGCRKL 420

Query: 429 QYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKL 488
           QYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKL
Sbjct: 421 QYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKL 480

Query: 489 TRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFG 548
           TRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAG+SDLGLK+VLEGCLRLQKLEIRDSPFG
Sbjct: 481 TRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGDSDLGLKFVLEGCLRLQKLEIRDSPFG 540

Query: 549 DVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLPRL 608
           + GL+SGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV                   
Sbjct: 541 NGGLQSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV------------------- 600

Query: 609 LGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
             EVMRNENDEE++NHIQVE MENHVQVLYMYRSLEGPRDDAPKFVDIL
Sbjct: 601 --EVMRNENDEEVDNHIQVEDMENHVQVLYMYRSLEGPRDDAPKFVDIL 621

BLAST of Spg016837 vs. ExPASy TrEMBL
Match: A0A6J1IZF2 (transport inhibitor response 1-like protein isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481282 PE=4 SV=1)

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 590/648 (91.05%), Postives = 605/648 (93.36%), Query Frame = 0

Query: 9   MSEDDDRSLPSDL-GGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFLTSR 68
           MSEDDDRSLP DL GG A+AESASKTRNCTGC GD+A SGSAEN LHNVLENVLHFLTSR
Sbjct: 1   MSEDDDRSLPLDLGGGGAVAESASKTRNCTGCYGDLAASGSAENTLHNVLENVLHFLTSR 60

Query: 69  RDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFNL 128
            DRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRF RVRSVTIKGRPRFSDFNL
Sbjct: 61  HDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFKRVRSVTIKGRPRFSDFNL 120

Query: 129 MPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFG 188
           MPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFG
Sbjct: 121 MPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFG 180

Query: 189 TSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCVECP 248
           TSGIAVVASRC       RQLRVLDLIESD+ADDEVDWISCFPEKET LESLVFDCVECP
Sbjct: 181 TSGIAVVASRC-------RQLRVLDLIESDIADDEVDWISCFPEKETYLESLVFDCVECP 240

Query: 249 INFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHGEPE 308
           INFEALEMLVSRSPLLKKLGVNRYVS+AQLYHL+VRAPQLTHLGTGSFCNPEAM+HGEPE
Sbjct: 241 INFEALEMLVSRSPLLKKLGVNRYVSIAQLYHLIVRAPQLTHLGTGSFCNPEAMIHGEPE 300

Query: 309 PDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIRHCH 368
           PDYAAAFAACKSLVCLSGFKDILPDYLP IYP CANLTTLNLSYANI+AEQLKPVI HCH
Sbjct: 301 PDYAAAFAACKSLVCLSGFKDILPDYLPFIYPACANLTTLNLSYANISAEQLKPVISHCH 360

Query: 369 KLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQ 428
           KL+TFWALDSICDEGLQAVASTCK+LQELRVFPVD REDGEGPISEVGFQAISEGCRKLQ
Sbjct: 361 KLQTFWALDSICDEGLQAVASTCKDLQELRVFPVDPREDGEGPISEVGFQAISEGCRKLQ 420

Query: 429 YILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLT 488
           YILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLT
Sbjct: 421 YILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLT 480

Query: 489 RLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGD 548
           RLAISGLLTDRAFSYIGKYGKLVRTLSVAFAG+SDLGLK+VLEGCLRLQKLEIRDSPFG+
Sbjct: 481 RLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGDSDLGLKFVLEGCLRLQKLEIRDSPFGN 540

Query: 549 VGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLPRLL 608
            GL+SGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV                    
Sbjct: 541 GGLQSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVV-------------------- 600

Query: 609 GEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
            EVMRNENDEE++NHIQVE MENHVQVLYMYRSLEGPRDDAPKFVDIL
Sbjct: 601 -EVMRNENDEEVDNHIQVEDMENHVQVLYMYRSLEGPRDDAPKFVDIL 620

BLAST of Spg016837 vs. ExPASy TrEMBL
Match: A0A6J1C2U2 (transport inhibitor response 1-like protein OS=Momordica charantia OX=3673 GN=LOC111007850 PE=4 SV=1)

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 584/665 (87.82%), Postives = 610/665 (91.73%), Query Frame = 0

Query: 9   MSEDDDRSLPSDLGGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVLENVLHFLTSRR 68
           MSEDDDRSL SDLG    A+SASK RNCTGCSGDV+GSGSAENILHNVLENVLHFLTSRR
Sbjct: 1   MSEDDDRSLTSDLGA---AQSASKARNCTGCSGDVSGSGSAENILHNVLENVLHFLTSRR 60

Query: 69  DRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKGRPRFSDFNLM 128
           DR+AASLVCKSWYRVEALTRS+LFIGNCYAVSP RVT+RF RVRSV+IKG+PRF+DFNLM
Sbjct: 61  DRSAASLVCKSWYRVEALTRSDLFIGNCYAVSPCRVTARFGRVRSVSIKGKPRFADFNLM 120

Query: 129 PPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFGT 188
           PPNWGAHF PWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFGT
Sbjct: 121 PPNWGAHFAPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKELVLFCCEGFGT 180

Query: 189 SGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLESLVFDCVECPI 248
           SGIAVVASRC       R LRVLDLIESDV DDEVDWISCFPEKETCLESLVFDCVECPI
Sbjct: 181 SGIAVVASRC-------RHLRVLDLIESDVTDDEVDWISCFPEKETCLESLVFDCVECPI 240

Query: 249 NFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNPEAMVHGEPEP 308
           NFEALEMLV+RSP LKKLGVNRY+S+ QLYHLM+RAP+LTHLGTGSFCNPEA+VHGE EP
Sbjct: 241 NFEALEMLVTRSPYLKKLGVNRYISITQLYHLMIRAPKLTHLGTGSFCNPEAIVHGELEP 300

Query: 309 DYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVIRHCHK 368
           DYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVI HCHK
Sbjct: 301 DYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQLKPVISHCHK 360

Query: 369 LRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQY 428
           L+TFWALDSICD+GLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQY
Sbjct: 361 LQTFWALDSICDDGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQAISEGCRKLQY 420

Query: 429 ILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLTR 488
           ILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLTR
Sbjct: 421 ILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGAIVINCKKLTR 480

Query: 489 LAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGDV 548
           LAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGD 
Sbjct: 481 LAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKLEIRDSPFGDG 540

Query: 549 GLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEMENHVLPRLLG 608
            LRSGLHHYYNMRFLWMS+CKLSRQGCQEVARAMPHLVVEVMRNEN E++ NH+    + 
Sbjct: 541 ALRSGLHHYYNMRFLWMSSCKLSRQGCQEVARAMPHLVVEVMRNENGEDINNHIQVEDME 600

Query: 609 EVMRNENDEE------------------MENHIQVEGMENHVQVLYMYRSLEGPRDDAPK 656
             ++ E+ EE                  +ENH+QVE MENHVQVLYMYRSLEGPRDDAP 
Sbjct: 601 NYVQVEDMEENHVQVEEDMEENHVQVEDVENHVQVEDMENHVQVLYMYRSLEGPRDDAPN 655

BLAST of Spg016837 vs. ExPASy TrEMBL
Match: A0A6J1J4M9 (transport inhibitor response 1-like protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481282 PE=4 SV=1)

HSP 1 Score: 1184.1 bits (3062), Expect = 0.0e+00
Identity = 591/672 (87.95%), Postives = 608/672 (90.48%), Query Frame = 0

Query: 1   MRVEQAEEMSEDDDRSLPSDLGGD-----------------AIAESASKTRNCTGCSGDV 60
           MRVEQ  EMS+D+DR    DLG D                 A+AESASKTRNCTGC GD+
Sbjct: 1   MRVEQV-EMSQDNDRPPTLDLGSDAAANKTRNCSGGGGGGGAVAESASKTRNCTGCYGDL 60

Query: 61  AGSGSAENILHNVLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRR 120
           A SGSAEN LHNVLENVLHFLTSR DRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRR
Sbjct: 61  AASGSAENTLHNVLENVLHFLTSRHDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRR 120

Query: 121 VTSRFNRVRSVTIKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDD 180
           VTSRF RVRSVTIKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDD
Sbjct: 121 VTSRFKRVRSVTIKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDD 180

Query: 181 DLALLAESFPGFKELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEV 240
           DLALLAESFPGFKELVLFCCEGFGTSGIAVVASRC       RQLRVLDLIESD+ADDEV
Sbjct: 181 DLALLAESFPGFKELVLFCCEGFGTSGIAVVASRC-------RQLRVLDLIESDIADDEV 240

Query: 241 DWISCFPEKETCLESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVR 300
           DWISCFPEKET LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVS+AQLYHL+VR
Sbjct: 241 DWISCFPEKETYLESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSIAQLYHLIVR 300

Query: 301 APQLTHLGTGSFCNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCAN 360
           APQLTHLGTGSFCNPEAM+HGEPEPDYAAAFAACKSLVCLSGFKDILPDYLP IYP CAN
Sbjct: 301 APQLTHLGTGSFCNPEAMIHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPFIYPACAN 360

Query: 361 LTTLNLSYANITAEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDA 420
           LTTLNLSYANI+AEQLKPVI HCHKL+TFWALDSICDEGLQAVASTCK+LQELRVFPVD 
Sbjct: 361 LTTLNLSYANISAEQLKPVISHCHKLQTFWALDSICDEGLQAVASTCKDLQELRVFPVDP 420

Query: 421 REDGEGPISEVGFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQ 480
           REDGEGPISEVGFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQ
Sbjct: 421 REDGEGPISEVGFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQ 480

Query: 481 PDHKTGEPMDEGFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDL 540
           PDHKTGEPMDEGFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAG+SDL
Sbjct: 481 PDHKTGEPMDEGFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGDSDL 540

Query: 541 GLKYVLEGCLRLQKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMP 600
           GLK+VLEGCLRLQKLEIRDSPFG+ GL+SGLHHYYNMRFLWMSACKLSRQGCQEVARAMP
Sbjct: 541 GLKFVLEGCLRLQKLEIRDSPFGNGGLQSGLHHYYNMRFLWMSACKLSRQGCQEVARAMP 600

Query: 601 HLVVEVMRNENDEEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEG 656
           HLVV                     EVMRNENDEE++NHIQVE MENHVQVLYMYRSLEG
Sbjct: 601 HLVV---------------------EVMRNENDEEVDNHIQVEDMENHVQVLYMYRSLEG 643

BLAST of Spg016837 vs. ExPASy TrEMBL
Match: A0A6J1IN53 (transport inhibitor response 1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111476739 PE=4 SV=1)

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 581/657 (88.43%), Postives = 604/657 (91.93%), Query Frame = 0

Query: 1   MRVEQAEEMSEDDDRSLPSDLGGDAIAESASKTRNCTGCSGDVAG--SGSAENILHNVLE 60
           MRV+Q+EEMSED+DRSLPSDL GDAIAES SK RNC GCSGDVAG  SGSAENILHNVLE
Sbjct: 1   MRVDQSEEMSEDEDRSLPSDLAGDAIAESVSKARNCVGCSGDVAGSDSGSAENILHNVLE 60

Query: 61  NVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIKG 120
           NVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSV IKG
Sbjct: 61  NVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVMIKG 120

Query: 121 RPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKEL 180
           +PRF+DFNLMPPNWGAHF+PWVAAMAKSYPWLE +YLKRMSVTDDDLALLA+SFPGFKEL
Sbjct: 121 KPRFADFNLMPPNWGAHFSPWVAAMAKSYPWLETVYLKRMSVTDDDLALLADSFPGFKEL 180

Query: 181 VLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLES 240
           VLFCCEGFGTSGIAVVASRC         LRVLDLIESDVADDEVDWISCFPEKETCLES
Sbjct: 181 VLFCCEGFGTSGIAVVASRC-------MHLRVLDLIESDVADDEVDWISCFPEKETCLES 240

Query: 241 LVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCNP 300
           L+FDC+ECPINFEALE LVSRSP LKKLGVNRYVS+AQLY LM+RAP LTHLGTGSF   
Sbjct: 241 LMFDCIECPINFEALERLVSRSPSLKKLGVNRYVSIAQLYRLMIRAPHLTHLGTGSFSTS 300

Query: 301 EAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAEQ 360
           EA+VHGE EPDYA+AFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLS+ANIT EQ
Sbjct: 301 EAVVHGESEPDYASAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSFANITPEQ 360

Query: 361 LKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQA 420
           LKPVI HCHKL+TFWALDSICDEGLQAVASTCKEL+ELRVFPVD REDGEGPISEVGFQA
Sbjct: 361 LKPVISHCHKLQTFWALDSICDEGLQAVASTCKELRELRVFPVDVREDGEGPISEVGFQA 420

Query: 421 ISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFGA 480
           ISEGCRKLQYILYFCQRMTNAAV+AMS+NCQDLVVFRLCIMGRHQPDH+TGEPMD+GFGA
Sbjct: 421 ISEGCRKLQYILYFCQRMTNAAVVAMSQNCQDLVVFRLCIMGRHQPDHQTGEPMDQGFGA 480

Query: 481 IVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKL 540
           IVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKL
Sbjct: 481 IVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQKL 540

Query: 541 EIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEEM 600
           EIRDSPFGD  LRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNEND+E 
Sbjct: 541 EIRDSPFGDGALRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDDET 600

Query: 601 ENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
           EN V                     +VEGMENHVQVLYMYRSLEGPRDDAPKFVDIL
Sbjct: 601 ENSV---------------------EVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 629

BLAST of Spg016837 vs. TAIR 10
Match: AT5G49980.1 (auxin F-box protein 5 )

HSP 1 Score: 892.9 bits (2306), Expect = 1.6e-259
Identity = 439/660 (66.52%), Postives = 530/660 (80.30%), Query Frame = 0

Query: 4   EQAEEMSEDDD--RSLPSDLGGDAIAE------SASKTRNCTGCSGDVAGSGSAENILHN 63
           +   EMSEDDD  +S P DL   AIA+      S +K+RNC   S         +++L N
Sbjct: 3   QDRSEMSEDDDDQQSPPLDLPSTAIADPCSSSSSPNKSRNCISNS-----QTFPDHVLEN 62

Query: 64  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 123
           VLENVL FL SR DRNAASLVCKSW+RVEALTRSE+FIGNCYA+SP R+T RF RVRS+ 
Sbjct: 63  VLENVLQFLDSRCDRNAASLVCKSWWRVEALTRSEVFIGNCYALSPARLTQRFKRVRSLV 122

Query: 124 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 183
           +KG+PRF+DFNLMPP+WGA+F PWV+ MA++YP LE++ LKRM VTDDDLALLA+SFPGF
Sbjct: 123 LKGKPRFADFNLMPPDWGANFAPWVSTMAQAYPCLEKVDLKRMFVTDDDLALLADSFPGF 182

Query: 184 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 243
           KEL+L CCEGFGTSGI++VA++C       R+L+VLDLIES+V DDEVDWISCFPE  TC
Sbjct: 183 KELILVCCEGFGTSGISIVANKC-------RKLKVLDLIESEVTDDEVDWISCFPEDVTC 242

Query: 244 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 303
           LESL FDCVE PINF+ALE LV+RSP LKKL +NR+VS+ +L+ L++ APQLT LGTGSF
Sbjct: 243 LESLAFDCVEAPINFKALEGLVARSPFLKKLRLNRFVSLVELHRLLLGAPQLTSLGTGSF 302

Query: 304 CNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANIT 363
            + E     E EPDYAAAF ACKS+VCLSGF++++P+YLP I+PVCANLT+LN SYANI+
Sbjct: 303 SHDEE-PQSEQEPDYAAAFRACKSVVCLSGFRELMPEYLPAIFPVCANLTSLNFSYANIS 362

Query: 364 AEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVG 423
            +  KP+I +CHKL+ FWALDSICDEGLQAVA+TCKEL+ELR+FP D RED EGP+SE+G
Sbjct: 363 PDMFKPIILNCHKLQVFWALDSICDEGLQAVAATCKELRELRIFPFDPREDSEGPVSELG 422

Query: 424 FQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEG 483
            QAISEGCRKL+ ILYFCQRMTNAAVIAMS+NC +L VFRLCIMGRH+PDH TG+PMDEG
Sbjct: 423 LQAISEGCRKLESILYFCQRMTNAAVIAMSENCPELTVFRLCIMGRHRPDHVTGKPMDEG 482

Query: 484 FGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRL 543
           FGAIV NCKKLTRLA+SGLLTD+AF Y+G+YGKLVRTLSVAFAG+SD+ L++VLEGC RL
Sbjct: 483 FGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLVRTLSVAFAGDSDMALRHVLEGCPRL 542

Query: 544 QKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNEND 603
           QKLEIRDSPFGDV LRSG+H YYNMRF+WMSAC LS+  C+++ARAMP+LVVEV+ +++D
Sbjct: 543 QKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACSLSKGCCKDIARAMPNLVVEVIGSDDD 602

Query: 604 EEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
           ++                                ++V+ LYMYRSL+GPR+DAPKFV IL
Sbjct: 603 DD------------------------------NRDYVETLYMYRSLDGPRNDAPKFVTIL 619

BLAST of Spg016837 vs. TAIR 10
Match: AT4G24390.2 (RNI-like superfamily protein )

HSP 1 Score: 854.4 bits (2206), Expect = 6.2e-248
Identity = 425/658 (64.59%), Postives = 509/658 (77.36%), Query Frame = 0

Query: 4   EQAEEMSEDDDRSL------PSDLGGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVL 63
           + + +MSED ++ L       S     + A   +K+RN        +     +++L NVL
Sbjct: 5   DSSAKMSEDVEKYLNLNPPCSSSSSSSSAATFTNKSRNFKS-----SPPPCPDHVLENVL 64

Query: 64  ENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIK 123
           ENVL FLTSR DRNA SLVC+SWYRVEA TR E+FIGNCY++SP R+  RF RVRS+ +K
Sbjct: 65  ENVLQFLTSRCDRNAVSLVCRSWYRVEAQTRLEVFIGNCYSLSPARLIHRFKRVRSLVLK 124

Query: 124 GRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKE 183
           G+PRF+DFNLMPPNWGA F+PWVAA AK+YPWLE+++LKRM VTDDDLALLAESFPGFKE
Sbjct: 125 GKPRFADFNLMPPNWGAQFSPWVAATAKAYPWLEKVHLKRMFVTDDDLALLAESFPGFKE 184

Query: 184 LVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLE 243
           L L CCEGFGTSGIA+VA++C       RQL+VLDL+ES+V DDE+DWISCFPE ET LE
Sbjct: 185 LTLVCCEGFGTSGIAIVANKC-------RQLKVLDLMESEVTDDELDWISCFPEGETHLE 244

Query: 244 SLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCN 303
           SL FDCVE PINF+ALE LV RSP LKKL  NR+VS+ +L+ LMVRAPQLT LGTGSF +
Sbjct: 245 SLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLTSLGTGSF-S 304

Query: 304 PEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAE 363
           P+ +  GE +PDYAAAF ACKS+VCLSGF++  P+YL  I  VCANLT+LN SYANI+  
Sbjct: 305 PDNVPQGEQQPDYAAAFRACKSIVCLSGFREFRPEYLLAISSVCANLTSLNFSYANISPH 364

Query: 364 QLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQ 423
            LKP+I +CH +R FWALDSI DEGLQAVA+TCKEL+ELR+FP D RED EGP+S VG Q
Sbjct: 365 MLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELRELRIFPFDPREDSEGPVSGVGLQ 424

Query: 424 AISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFG 483
           AISEGCRKL+ ILYFCQ MTN AV AMS+NC  L VFRLCIMGRH+PDH TG+PMD+GFG
Sbjct: 425 AISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVFRLCIMGRHRPDHVTGKPMDDGFG 484

Query: 484 AIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQK 543
           AIV NCKKLTRLA+SGLLTD AFSYIG+YGKL+RTLSVAFAGNSD  L+YVLEGC +LQK
Sbjct: 485 AIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLSVAFAGNSDKALRYVLEGCPKLQK 544

Query: 544 LEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEE 603
           LEIRDSPFGDVGLRSG+H Y NMRF+W+S+C +SR GC+ V+ A+P++VVEV   + D++
Sbjct: 545 LEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGGCRGVSHALPNVVVEVFGADGDDD 604

Query: 604 MENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
            +                         V G  ++V+ LY+YRSL+GPR DAPKFV IL
Sbjct: 605 EDT------------------------VTG--DYVETLYLYRSLDGPRKDAPKFVTIL 623

BLAST of Spg016837 vs. TAIR 10
Match: AT4G24390.1 (RNI-like superfamily protein )

HSP 1 Score: 854.4 bits (2206), Expect = 6.2e-248
Identity = 425/658 (64.59%), Postives = 509/658 (77.36%), Query Frame = 0

Query: 4   EQAEEMSEDDDRSL------PSDLGGDAIAESASKTRNCTGCSGDVAGSGSAENILHNVL 63
           + + +MSED ++ L       S     + A   +K+RN        +     +++L NVL
Sbjct: 5   DSSAKMSEDVEKYLNLNPPCSSSSSSSSAATFTNKSRNFKS-----SPPPCPDHVLENVL 64

Query: 64  ENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVTIK 123
           ENVL FLTSR DRNA SLVC+SWYRVEA TR E+FIGNCY++SP R+  RF RVRS+ +K
Sbjct: 65  ENVLQFLTSRCDRNAVSLVCRSWYRVEAQTRLEVFIGNCYSLSPARLIHRFKRVRSLVLK 124

Query: 124 GRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGFKE 183
           G+PRF+DFNLMPPNWGA F+PWVAA AK+YPWLE+++LKRM VTDDDLALLAESFPGFKE
Sbjct: 125 GKPRFADFNLMPPNWGAQFSPWVAATAKAYPWLEKVHLKRMFVTDDDLALLAESFPGFKE 184

Query: 184 LVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETCLE 243
           L L CCEGFGTSGIA+VA++C       RQL+VLDL+ES+V DDE+DWISCFPE ET LE
Sbjct: 185 LTLVCCEGFGTSGIAIVANKC-------RQLKVLDLMESEVTDDELDWISCFPEGETHLE 244

Query: 244 SLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSFCN 303
           SL FDCVE PINF+ALE LV RSP LKKL  NR+VS+ +L+ LMVRAPQLT LGTGSF +
Sbjct: 245 SLSFDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLTSLGTGSF-S 304

Query: 304 PEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSYANITAE 363
           P+ +  GE +PDYAAAF ACKS+VCLSGF++  P+YL  I  VCANLT+LN SYANI+  
Sbjct: 305 PDNVPQGEQQPDYAAAFRACKSIVCLSGFREFRPEYLLAISSVCANLTSLNFSYANISPH 364

Query: 364 QLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEVGFQ 423
            LKP+I +CH +R FWALDSI DEGLQAVA+TCKEL+ELR+FP D RED EGP+S VG Q
Sbjct: 365 MLKPIISNCHNIRVFWALDSIRDEGLQAVAATCKELRELRIFPFDPREDSEGPVSGVGLQ 424

Query: 424 AISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDEGFG 483
           AISEGCRKL+ ILYFCQ MTN AV AMS+NC  L VFRLCIMGRH+PDH TG+PMD+GFG
Sbjct: 425 AISEGCRKLESILYFCQNMTNGAVTAMSENCPQLTVFRLCIMGRHRPDHVTGKPMDDGFG 484

Query: 484 AIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLRLQK 543
           AIV NCKKLTRLA+SGLLTD AFSYIG+YGKL+RTLSVAFAGNSD  L+YVLEGC +LQK
Sbjct: 485 AIVKNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLSVAFAGNSDKALRYVLEGCPKLQK 544

Query: 544 LEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNENDEE 603
           LEIRDSPFGDVGLRSG+H Y NMRF+W+S+C +SR GC+ V+ A+P++VVEV   + D++
Sbjct: 545 LEIRDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGGCRGVSHALPNVVVEVFGADGDDD 604

Query: 604 MENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDIL 656
            +                         V G  ++V+ LY+YRSL+GPR DAPKFV IL
Sbjct: 605 EDT------------------------VTG--DYVETLYLYRSLDGPRKDAPKFVTIL 623

BLAST of Spg016837 vs. TAIR 10
Match: AT3G26810.1 (auxin signaling F-box 2 )

HSP 1 Score: 564.3 bits (1453), Expect = 1.3e-160
Identity = 296/601 (49.25%), Postives = 384/601 (63.89%), Query Frame = 0

Query: 56  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 115
           V+E+V  F+TS +DRNA SLVCKSWY++E  +R ++FIGNCYA++P R+  RF  ++S+T
Sbjct: 8   VIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCLKSLT 67

Query: 116 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 175
           +KG+P F+DFNL+P  WG    PW+ A+A+S   LE + LKRM VTD+ L LL+ SF  F
Sbjct: 68  LKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRSFVNF 127

Query: 176 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 235
           K LVL  CEGF T G+A +A+ C       R LR LDL E+++ D    W+SCFP+  T 
Sbjct: 128 KSLVLVSCEGFTTDGLASIAANC-------RHLRDLDLQENEIDDHRGQWLSCFPDTCTT 187

Query: 236 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 295
           L +L F C+E   N  ALE LV+RSP LK L +NR V +  L  LM  APQ+  LG GS+
Sbjct: 188 LVTLNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSY 247

Query: 296 CNPEAMVHGEPEPDYAAAFAACKSLVCLSGFKDILPDYLPCIYPVCANLTTLNLSY-ANI 355
            N       E      A    C SL  LSGF +  P  L   +P+C NLT+LNLSY A I
Sbjct: 248 ENDP---DSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEI 307

Query: 356 TAEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVDAREDGEGPISEV 415
               L  +I+HC KL+  W LDSI D+GL+ VASTCKELQELRVFP D    G   ++E 
Sbjct: 308 HGSHLIKLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNTAVTEE 367

Query: 416 GFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKTGEPMDE 475
           G  AIS GC KL  ILYFCQ+MTNAA++ ++KNC + + FRLCI+  ++PDH T +P+DE
Sbjct: 368 GLVAISAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDE 427

Query: 476 GFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYVLEGCLR 535
           GFGAIV  CK L RL++SGLLTD+ F YIG Y   +  LS+AFAG++D G+ YVL GC +
Sbjct: 428 GFGAIVKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKK 487

Query: 536 LQKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVEVMRNEN 595
           ++KLEIRDSPFGD  L + +  Y  MR LWMS+C+++  GC+ +A   P L VE++ NEN
Sbjct: 488 MKKLEIRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEII-NEN 547

Query: 596 DEEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDAPKFVDI 655
           D                   N  E   H   EG +  V  LY+YR++ G R DAP FV I
Sbjct: 548 D------------------NNRMEENGH---EGRQK-VDKLYLYRTVVGTRMDAPPFVWI 575

BLAST of Spg016837 vs. TAIR 10
Match: AT1G12820.1 (auxin signaling F-box 3 )

HSP 1 Score: 557.4 bits (1435), Expect = 1.6e-158
Identity = 293/607 (48.27%), Postives = 390/607 (64.25%), Query Frame = 0

Query: 56  VLENVLHFLTSRRDRNAASLVCKSWYRVEALTRSELFIGNCYAVSPRRVTSRFNRVRSVT 115
           V+E+V  F+ S +DRN+ SLVCKSW+++E  +R E+FIGNCYA++P R+  RF  ++S+T
Sbjct: 8   VIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRFPCLKSLT 67

Query: 116 IKGRPRFSDFNLMPPNWGAHFTPWVAAMAKSYPWLERIYLKRMSVTDDDLALLAESFPGF 175
           +KG+P F+DFNL+P  WG    PW+ A+A+S   LE + LKRM VTD+ L LL+ SF  F
Sbjct: 68  LKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLLSRSFANF 127

Query: 176 KELVLFCCEGFGTSGIAVVASRCRLLDLSFRQLRVLDLIESDVADDEVDWISCFPEKETC 235
           K LVL  CEGF T G+A +A+ C       R LR LDL E+++ D    W++CFP+  T 
Sbjct: 128 KSLVLVSCEGFTTDGLASIAANC-------RHLRELDLQENEIDDHRGQWLNCFPDSCTT 187

Query: 236 LESLVFDCVECPINFEALEMLVSRSPLLKKLGVNRYVSMAQLYHLMVRAPQLTHLGTGSF 295
           L SL F C++   N  ALE LV+RSP LK L +NR V +  L  LM  APQL  LG GS+
Sbjct: 188 LMSLNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSY 247

Query: 296 CNPEAMVHGEPEPD-YAAAFAACK---SLVCLSGFKDILPDYLPCIYPVCANLTTLNLSY 355
            N       EP+P+ +A    A K   SL  LSGF ++ P  LP  YP+C NL +LNLSY
Sbjct: 248 EN-------EPDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSY 307

Query: 356 -ANITAEQLKPVIRHCHKLRTFWALDSICDEGLQAVASTCKELQELRVFPVD--AREDGE 415
            A I    L  +I+ C +L+  W LDSI D+GL  VA+TCKELQELRVFP D    ED  
Sbjct: 308 AAEIQGNHLIKLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSDVHGEEDNN 367

Query: 416 GPISEVGFQAISEGCRKLQYILYFCQRMTNAAVIAMSKNCQDLVVFRLCIMGRHQPDHKT 475
             ++EVG  AIS GC KL  ILYFC++MTNAA+IA++KNC + + FRLCI+  H+PDH T
Sbjct: 368 ASVTEVGLVAISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHIT 427

Query: 476 GEPMDEGFGAIVINCKKLTRLAISGLLTDRAFSYIGKYGKLVRTLSVAFAGNSDLGLKYV 535
            + +DEGFGAIV  CK L RL++SGLLTD+ F YIG Y + +  LS+AFAG++D G+ YV
Sbjct: 428 FQSLDEGFGAIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYV 487

Query: 536 LEGCLRLQKLEIRDSPFGDVGLRSGLHHYYNMRFLWMSACKLSRQGCQEVARAMPHLVVE 595
           L GC +++KLEIRDSPFG+  L + +  Y  MR LWMS+C+++  GC+ +A+  P L VE
Sbjct: 488 LNGCKKMRKLEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVE 547

Query: 596 VMRNENDEEMENHVLPRLLGEVMRNENDEEMENHIQVEGMENHVQVLYMYRSLEGPRDDA 655
           ++    +  ME            +NE DE              V  LY+YR++ G R DA
Sbjct: 548 IINENENNGME------------QNEEDE-----------REKVDKLYLYRTVVGTRKDA 577

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023524117.10.0e+0090.15transport inhibitor response 1-like protein [Cucurbita pepo subsp. pepo][more]
XP_022941095.10.0e+0090.76transport inhibitor response 1-like protein [Cucurbita moschata] >XP_022941097.1... [more]
XP_022982471.10.0e+0091.05transport inhibitor response 1-like protein isoform X3 [Cucurbita maxima] >XP_02... [more]
KAG7037613.10.0e+0090.32Transport inhibitor response 1-like protein, partial [Cucurbita argyrosperma sub... [more]
XP_022136070.10.0e+0087.82transport inhibitor response 1-like protein [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9LTX22.2e-25866.52Transport inhibitor response 1-like protein OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q8RWQ88.7e-24764.59F-box protein FBX14 OS=Arabidopsis thaliana OX=3702 GN=FBX14 PE=1 SV=1[more]
Q9LW291.8e-15949.25Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=... [more]
Q7XVM89.8e-15849.25Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. ... [more]
Q9LPW72.2e-15748.27Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1FR480.0e+0090.76transport inhibitor response 1-like protein OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IZF20.0e+0091.05transport inhibitor response 1-like protein isoform X3 OS=Cucurbita maxima OX=36... [more]
A0A6J1C2U20.0e+0087.82transport inhibitor response 1-like protein OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1J4M90.0e+0087.95transport inhibitor response 1-like protein isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1IN530.0e+0088.43transport inhibitor response 1-like protein OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT5G49980.11.6e-25966.52auxin F-box protein 5 [more]
AT4G24390.26.2e-24864.59RNI-like superfamily protein [more]
AT4G24390.16.2e-24864.59RNI-like superfamily protein [more]
AT3G26810.11.3e-16049.25auxin signaling F-box 2 [more]
AT1G12820.11.6e-15848.27auxin signaling F-box 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 483..506
e-value: 20.0
score: 12.1
coord: 172..197
e-value: 550.0
score: 0.9
coord: 423..447
e-value: 8.0
score: 15.3
coord: 532..556
e-value: 280.0
score: 3.3
IPR041101Transport inhibitor response 1 domainPFAMPF18791Transp_inhibitcoord: 110..155
e-value: 1.4E-24
score: 85.4
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 90..655
e-value: 6.4E-151
score: 505.2
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 341..357
e-value: 0.85
score: 9.9
NoneNo IPR availableGENE3D1.20.1280.50coord: 45..88
e-value: 8.0E-16
score: 59.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR16134F-BOX/TPR REPEAT PROTEIN POF3coord: 43..654
NoneNo IPR availablePANTHERPTHR16134:SF36F-BOX PROTEIN FBX14coord: 43..654
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 76..597
IPR041567COI1, F-boxPFAMPF18511F-box_5coord: 51..89
e-value: 1.8E-20
score: 72.2
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 55..83

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016837.1Spg016837.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component GO:0019005 SCF ubiquitin ligase complex
molecular_function GO:0005515 protein binding