Spg016831 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016831 vs. NCBI nr
Match: KAG6608306.1 (Transmembrane 9 superfamily member 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 205.7 bits (522), Expect = 5.4e-49
Identity = 166/470 (35.32%), Postives = 194/470 (41.28%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASRSS   WTLTI L FLLLVH+ HCFN FG+ PVD +KGD LKVKVKGLTSTKTQLPI
Sbjct: 1   MASRSSLSFWTLTIPLTFLLLVHSVHCFNLFGIRPVDFKKGDDLKVKVKGLTSTKTQLPI 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCGLA------------- 145
           S+YSL      K +DDA ++GEILLG               + C +              
Sbjct: 61  SYYSLPFCRPNKIQDDAENLGEILLGDRRDNSPYEAKMMEFQLCNIVCRITLDEKGAKAL 120

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 121 KEKIEDDYMVHMTLDSLPLVRPIRIFEHESPFTYQLGFHMGLKGVKHEYKGAWKERNTRL 180

Query: 206 ------------------------------------------FRWDAYHATRDDETRWFS 265
                                                      RWDAY ATRDD+T WFS
Sbjct: 181 STCDPKWKAMVLNSDGPQEVEEGKEIIYTYNVDYQESDVDWPSRWDAYLATRDDQTHWFS 240

Query: 266 IMNGLESILLISGILAVIVGR--------------------------------------- 284
           I+NGLESIL+ISGILAVIV R                                       
Sbjct: 241 ILNGLESILVISGILAVIVWRIYRDIFNYNDLETQDRVQKETGWKLIHGDVFRPPCNSDL 300

BLAST of Spg016831 vs. NCBI nr
Match: XP_022940264.1 (transmembrane 9 superfamily member 8-like [Cucurbita moschata])

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-44
Identity = 169/512 (33.01%), Postives = 196/512 (38.28%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASRSS  VWTLTI L FLLLVH+ HCFN FG+ PVD +KGD LKVKVKGLTSTKTQLPI
Sbjct: 1   MASRSSLSVWTLTIPLTFLLLVHSVHCFNLFGIRPVDFKKGDDLKVKVKGLTSTKTQLPI 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCG--------------- 145
           S+YSL      K +DDA ++GEILLG               + C                
Sbjct: 61  SYYSLPFCRPNKIQDDAENLGEILLGDRRDNSPYEAKMMEFQLCNIVCRITLDEKGAKAL 120

Query: 146 -----------------------------------------------------------L 205
                                                                      L
Sbjct: 121 KEKIEDDYMVHMTLDSLPLVRPIRIFEHESPFTYQLGFHMGLKGYYPEEKAEKYFIYNHL 180

Query: 206 AF---------------------------------------------------------- 265
           AF                                                          
Sbjct: 181 AFTIKYHVDTQSNSTRIVGFEVQPFSVKHEYKGAWKERNTRLSTCDPKWKAMVLNSDGPQ 240

Query: 266 -------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVI 284
                                    RWDAY ATRDD+T WFSI+NGLESIL+ISGILAVI
Sbjct: 241 EVEEGKEIIYTYNVDYQESDVDWPSRWDAYLATRDDQTHWFSILNGLESILVISGILAVI 300

BLAST of Spg016831 vs. NCBI nr
Match: KAG7037653.1 (Transmembrane 9 superfamily member 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-42
Identity = 167/512 (32.62%), Postives = 194/512 (37.89%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASRSS   WTLTI L FLLLVH+ HCFN FG+ PVD +KGD LKVKVKGLTSTKTQLPI
Sbjct: 1   MASRSSLSFWTLTIPLTFLLLVHSVHCFNLFGIRPVDFKKGDDLKVKVKGLTSTKTQLPI 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCG--------------- 145
           S+YSL      K +DDA ++GEILLG               + C                
Sbjct: 61  SYYSLPFCRPNKIQDDAENLGEILLGDRRDNSPYEAKMMEFQLCNIVCRITLDEKGAKAL 120

Query: 146 -----------------------------------------------------------L 205
                                                                      L
Sbjct: 121 KEKIEDDYMVHMTLDSLPLVRPIRIFEHESPFTYQLGFHMGLKGYYPEEKAEKYFIYNHL 180

Query: 206 AF---------------------------------------------------------- 265
           AF                                                          
Sbjct: 181 AFTIKYHVDTQSNSTRIVGFEVQPFSVKHEYKGAWKERNTRLSTCDPKWKAMVLNSDGPQ 240

Query: 266 -------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVI 284
                                    RWDAY ATRDD+T WFSI+NGLESIL+ISGILAVI
Sbjct: 241 EVEEGKEIIYTYNVDYQESDVDWPSRWDAYLATRDDQTHWFSILNGLESILVISGILAVI 300

BLAST of Spg016831 vs. NCBI nr
Match: XP_038896919.1 (transmembrane 9 superfamily member 9-like [Benincasa hispida])

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-42
Identity = 169/512 (33.01%), Postives = 195/512 (38.09%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASRSS  V TLTISL+FLLL H  HCFNFFG+ PVD +KGD LKVKVKGLTSTKTQLP+
Sbjct: 1   MASRSSLPVRTLTISLSFLLLFHYVHCFNFFGIRPVDFKKGDDLKVKVKGLTSTKTQLPV 60

Query: 86  SHYSL-----EKREDDAVDIGEILLGE---------------CCG--------------- 145
           S+YSL     EK EDDA ++GEILLG+                C                
Sbjct: 61  SYYSLPFCRPEKIEDDAENLGEILLGDRSQNSPYVAKMLEHRLCNIICRIELDSEGAEKL 120

Query: 146 -------------------------------LAF-------------------------- 205
                                          LA+                          
Sbjct: 121 KKKIEDEYMVQMILDNLPLVHPIRIFEHESPLAYQLGFHMGLKGYYSEEQAEKYFIYNHL 180

Query: 206 ------------------------------------------------------------ 265
                                                                       
Sbjct: 181 LFTIKYYHDLRANSTRIVGFEVKPFSIKHEYNGKWKERNTRLSTCDPISKVTVINSDGPQ 240

Query: 266 -------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVI 284
                                    RWDAY ATRDD+  WFSI+NGLESIL+ISGILAVI
Sbjct: 241 VVEEGKEIIFTYDIEYQESEVDWPARWDAYLATRDDQMHWFSILNGLESILVISGILAVI 300

BLAST of Spg016831 vs. NCBI nr
Match: XP_022981837.1 (transmembrane 9 superfamily member 8-like [Cucurbita maxima] >XP_022981838.1 transmembrane 9 superfamily member 8-like [Cucurbita maxima])

HSP 1 Score: 177.9 bits (450), Expect = 1.2e-40
Identity = 165/512 (32.23%), Postives = 194/512 (37.89%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASRSS  V TLTI L+FLLLVH+ HCFN FG+ PV  +KGD LKVKVKGLTSTKTQLPI
Sbjct: 1   MASRSSLSVRTLTIPLSFLLLVHSVHCFNLFGIRPVAFKKGDELKVKVKGLTSTKTQLPI 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCG--------------- 145
           S+YSL      K +DDA ++GEILLG               + C                
Sbjct: 61  SYYSLPFCRPNKIQDDAENLGEILLGDRRDNSPYEAKMMEFQLCNIVCRIKLDEKGAKAL 120

Query: 146 -----------------------------------------------------------L 205
                                                                      L
Sbjct: 121 KEKIEDDYMVHMTLDSLPLVRPIRIFEHESPFTYQLGFHMGLKGYYPEEKTEKYFIYNHL 180

Query: 206 AF---------------------------------------------------------- 265
           AF                                                          
Sbjct: 181 AFTIKYHVDTQSNSTRIVGFEVQPFSVKHEYKGAWKERNTRLSTCDPKWKTTVLNSDGPQ 240

Query: 266 -------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVI 284
                                    RWDAY ATRDD+T WFSI+NGLESIL+ISGILAVI
Sbjct: 241 EVEEGKEIIYTYNVDYQESDVDWSSRWDAYLATRDDQTHWFSILNGLESILVISGILAVI 300

BLAST of Spg016831 vs. ExPASy Swiss-Prot
Match: Q9C5N2 (Transmembrane 9 superfamily member 9 OS=Arabidopsis thaliana OX=3702 GN=TMN9 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 4.2e-20
Identity = 83/245 (33.88%), Postives = 112/245 (45.71%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVI--------VGRFTVIFI------ 171
           A RWDAY    D++  WFSI+N L  +L +SG++A+I        + R+  +        
Sbjct: 266 ASRWDAYLLMSDNQIHWFSIVNSLMIVLFLSGMVAMIMLRTLYRDISRYNELETQEEAQE 325

Query: 172 ----------MVQPLSNADL----------------------ELSFKS--------TTML 231
                     + +P +N+DL                       L F S        T ML
Sbjct: 326 ETGWKLVHGDVFRPPANSDLLCVYVGTGVQCLGMVLVTMIFAMLGFLSPSNRGGLMTAML 385

Query: 232 WLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKSY- 284
            LW+FM L  GY S+R YKMF  T WK+I+ +  AF FP V+  IFF+LNAL+WG KS  
Sbjct: 386 LLWVFMGLFAGYASSRLYKMFKGTEWKRIAFR-TAFLFPAVVSAIFFVLNALIWGQKSSG 445

HSP 2 Score: 68.9 bits (167), Expect = 1.0e-10
Identity = 38/73 (52.05%), Postives = 46/73 (63.01%), Query Frame = 0

Query: 40  SLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPISHYSL-----EKRE 99
           S+  LL +H AH F   GV P D EKGD LKVKV  LTS KTQLP S+YSL     +K  
Sbjct: 15  SVILLLSIHVAHSFYLPGVAPQDFEKGDELKVKVNKLTSIKTQLPYSYYSLPFCRPKKIV 74

Query: 100 DDAVDIGEILLGE 108
           D   ++GE+L G+
Sbjct: 75  DSTENLGEVLRGD 87

BLAST of Spg016831 vs. ExPASy Swiss-Prot
Match: F4KIB2 (Transmembrane 9 superfamily member 8 OS=Arabidopsis thaliana OX=3702 GN=TMN8 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.9e-18
Identity = 79/247 (31.98%), Postives = 109/247 (44.13%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVIVGR-------------------- 171
           A RWD Y    D++  WFSI+N L  +L +SG++A+I+ R                    
Sbjct: 270 ASRWDTYLLMSDNQIHWFSIVNSLMIVLFLSGMVAMIMLRTLYRDISRYNELETQEEAQE 329

Query: 172 ------------------------------------FTVIFIMVQPLSNADLELSFKSTT 231
                                                T+IF M+  LS ++       T 
Sbjct: 330 ETGWKLVHGDVFRLPTNSDLLCVYVGTGVQCLGMVFVTMIFAMLGFLSPSN--RGGLMTA 389

Query: 232 MLWLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKS 284
           ML LW+FM L  GY S+R YKMF  T WK+I+ +  AF FP V+  IFF+LNAL+WG KS
Sbjct: 390 MLLLWVFMGLFAGYASSRLYKMFKGTEWKRIAFR-TAFLFPAVVSAIFFVLNALIWGQKS 449

HSP 2 Score: 73.2 bits (178), Expect = 5.5e-12
Identity = 42/86 (48.84%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 27  ASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPIS 86
           +SR   +     I+L FLL +H AH F   GV P D EKGD LKVKV  LTS KTQLP S
Sbjct: 8   SSRRILESSGCAIALIFLLFIHGAHSFYLPGVAPQDFEKGDELKVKVNKLTSIKTQLPYS 67

Query: 87  HYSL-----EKREDDAVDIGEILLGE 108
           +YSL      K  D   ++GE+L G+
Sbjct: 68  YYSLPFCRPSKIVDSTENLGEVLRGD 93

BLAST of Spg016831 vs. ExPASy Swiss-Prot
Match: Q9C720 (Transmembrane 9 superfamily member 6 OS=Arabidopsis thaliana OX=3702 GN=TMN6 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.8e-16
Identity = 73/247 (29.55%), Postives = 108/247 (43.72%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVIVGR-------------------- 171
           A RWD Y    DD+  WFSI+N L  +L +SG++A+I+ R                    
Sbjct: 259 ASRWDTYLLMNDDQIHWFSIINSLMIVLFLSGMVAMIMMRTLYKDISNYNQLETQDEAQE 318

Query: 172 ------------------------------------FTVIFIMVQPLSNADLELSFKSTT 231
                                                T+IF ++  LS ++      +T 
Sbjct: 319 ETGWKLVHGDVFRTPMNSGLLCVYVGTGVQIFGMTLVTMIFALLGFLSPSN--RGGLTTA 378

Query: 232 MLWLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKS 284
           M+ LW+FM +  GY S+R +KMF    WK+I+LK  AF FP +++ IFF+LN L+WG +S
Sbjct: 379 MVLLWVFMGIFAGYSSSRLHKMFKGNEWKRITLK-TAFMFPGILFAIFFVLNTLIWGERS 438

HSP 2 Score: 50.8 bits (120), Expect = 2.9e-05
Identity = 32/75 (42.67%), Postives = 41/75 (54.67%), Query Frame = 0

Query: 38  TISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPISHYSLE-----K 97
           T+ L+FL      H F   GV P D +KGD L VKV  L+STKTQLP   Y L      K
Sbjct: 10  TLLLSFLFF-STLHAFYLPGVAPRDFQKGDPLYVKVNKLSSTKTQLPYDFYYLNYCKPPK 69

Query: 98  REDDAVDIGEILLGE 108
             +   ++GE+L G+
Sbjct: 70  ILNTGENLGEVLRGD 83

BLAST of Spg016831 vs. ExPASy Swiss-Prot
Match: Q8RWW1 (Transmembrane 9 superfamily member 10 OS=Arabidopsis thaliana OX=3702 GN=TMN10 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 8.2e-16
Identity = 78/245 (31.84%), Postives = 106/245 (43.27%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVIVGRFTVIFI-------------- 171
           A RWD Y    DD+  WFSI+N +  +L +SG++A+I+ R     I              
Sbjct: 259 ASRWDTYLLMADDQIHWFSIVNSMMIVLFLSGMVAMIMLRTLYRDISNYNQLESHEEALE 318

Query: 172 ----------MVQPLSNADLE----------------------LSFKS--------TTML 231
                     + +P +N +L                       L F S        T ML
Sbjct: 319 ETGWKLVHGDVFRPPTNPELLCVYAGTGVQCFGMILVTMIFACLGFLSPSNRGGLMTAML 378

Query: 232 WLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKSY- 284
            LW+FM L  GY S+R YK    T WK+ +LK  AF FP  ++V FF+LNA++WG KS  
Sbjct: 379 LLWVFMGLLAGYASSRLYKTLRGTEWKRNALK-TAFMFPATVFVAFFVLNAIIWGQKSSG 438

HSP 2 Score: 61.2 bits (147), Expect = 2.2e-08
Identity = 38/80 (47.50%), Postives = 47/80 (58.75%), Query Frame = 0

Query: 33  QVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPISHYSL-- 92
           ++   T+ L F L VH  H F   GV P D + GD L VKV  LTSTKTQLP S+YSL  
Sbjct: 5   RILIFTLVLFFSLNVH-IHGFYLPGVAPQDFQMGDALMVKVNKLTSTKTQLPYSYYSLPY 64

Query: 93  ---EKREDDAVDIGEILLGE 108
              E   D A ++GE+L G+
Sbjct: 65  CRPEHIVDSAENLGEVLRGD 83

BLAST of Spg016831 vs. ExPASy Swiss-Prot
Match: Q9LIC2 (Transmembrane 9 superfamily member 7 OS=Arabidopsis thaliana OX=3702 GN=TMN7 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-15
Identity = 78/245 (31.84%), Postives = 109/245 (44.49%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVIVGRFTVIFI-------------- 171
           A RWD Y    DD+  WFSI+N L  +L +SG++A+I+ R     I              
Sbjct: 263 ASRWDTYLLMNDDQIHWFSIINSLMIVLFLSGMVAMIMMRTLYKDISNYNQLETQDEAQE 322

Query: 172 ----------MVQPLSNADL----------------------ELSFKS--------TTML 231
                     + +P  N+ L                       L F S        T M+
Sbjct: 323 ETGWKLVHGDVFRPPVNSGLLCVYVGTGVQIFGMSLVTMMFALLGFLSPSNRGGLMTAMV 382

Query: 232 WLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKSY- 284
            LW+FM +  GY S+R +KMF    WK+++LK  AF FP +++ IFF+LNAL+WG +S  
Sbjct: 383 LLWVFMGIFAGYSSSRLHKMFKGNKWKRMTLK-TAFMFPGILFAIFFVLNALIWGEQSSG 442

HSP 2 Score: 52.4 bits (124), Expect = 1.0e-05
Identity = 35/83 (42.17%), Postives = 49/83 (59.04%), Query Frame = 0

Query: 30  SSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPISHYS 89
           SSF+ +  T+ L+FL     +  F   GV P D +KGD L VKV  L+STKTQLP  +Y 
Sbjct: 7   SSFRFYA-TLLLSFLSF-SLSRAFYLPGVAPRDFQKGDPLYVKVNKLSSTKTQLPYDYYY 66

Query: 90  LE-----KREDDAVDIGEILLGE 108
           L      K  ++A ++GE+L G+
Sbjct: 67  LNYCKPPKILNNAENLGEVLRGD 87

BLAST of Spg016831 vs. ExPASy TrEMBL
Match: A0A6J1FJK2 (Transmembrane 9 superfamily member OS=Cucurbita moschata OX=3662 GN=LOC111445939 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 1.1e-44
Identity = 169/512 (33.01%), Postives = 196/512 (38.28%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASRSS  VWTLTI L FLLLVH+ HCFN FG+ PVD +KGD LKVKVKGLTSTKTQLPI
Sbjct: 1   MASRSSLSVWTLTIPLTFLLLVHSVHCFNLFGIRPVDFKKGDDLKVKVKGLTSTKTQLPI 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCG--------------- 145
           S+YSL      K +DDA ++GEILLG               + C                
Sbjct: 61  SYYSLPFCRPNKIQDDAENLGEILLGDRRDNSPYEAKMMEFQLCNIVCRITLDEKGAKAL 120

Query: 146 -----------------------------------------------------------L 205
                                                                      L
Sbjct: 121 KEKIEDDYMVHMTLDSLPLVRPIRIFEHESPFTYQLGFHMGLKGYYPEEKAEKYFIYNHL 180

Query: 206 AF---------------------------------------------------------- 265
           AF                                                          
Sbjct: 181 AFTIKYHVDTQSNSTRIVGFEVQPFSVKHEYKGAWKERNTRLSTCDPKWKAMVLNSDGPQ 240

Query: 266 -------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVI 284
                                    RWDAY ATRDD+T WFSI+NGLESIL+ISGILAVI
Sbjct: 241 EVEEGKEIIYTYNVDYQESDVDWPSRWDAYLATRDDQTHWFSILNGLESILVISGILAVI 300

BLAST of Spg016831 vs. ExPASy TrEMBL
Match: A0A6J1J362 (Transmembrane 9 superfamily member OS=Cucurbita maxima OX=3661 GN=LOC111480864 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 5.8e-41
Identity = 165/512 (32.23%), Postives = 194/512 (37.89%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASRSS  V TLTI L+FLLLVH+ HCFN FG+ PV  +KGD LKVKVKGLTSTKTQLPI
Sbjct: 1   MASRSSLSVRTLTIPLSFLLLVHSVHCFNLFGIRPVAFKKGDELKVKVKGLTSTKTQLPI 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCG--------------- 145
           S+YSL      K +DDA ++GEILLG               + C                
Sbjct: 61  SYYSLPFCRPNKIQDDAENLGEILLGDRRDNSPYEAKMMEFQLCNIVCRIKLDEKGAKAL 120

Query: 146 -----------------------------------------------------------L 205
                                                                      L
Sbjct: 121 KEKIEDDYMVHMTLDSLPLVRPIRIFEHESPFTYQLGFHMGLKGYYPEEKTEKYFIYNHL 180

Query: 206 AF---------------------------------------------------------- 265
           AF                                                          
Sbjct: 181 AFTIKYHVDTQSNSTRIVGFEVQPFSVKHEYKGAWKERNTRLSTCDPKWKTTVLNSDGPQ 240

Query: 266 -------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVI 284
                                    RWDAY ATRDD+T WFSI+NGLESIL+ISGILAVI
Sbjct: 241 EVEEGKEIIYTYNVDYQESDVDWSSRWDAYLATRDDQTHWFSILNGLESILVISGILAVI 300

BLAST of Spg016831 vs. ExPASy TrEMBL
Match: A0A6J1BTW1 (Transmembrane 9 superfamily member OS=Momordica charantia OX=3673 GN=LOC111004741 PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 4.9e-40
Identity = 163/502 (32.47%), Postives = 192/502 (38.25%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           M S SS  V TL ISL+FLLLVHA HCF FFGVHPVD ++GD LKVKVKGLTSTKTQLP+
Sbjct: 1   MPSPSSSPVRTLPISLSFLLLVHAVHCFYFFGVHPVDFKEGDDLKVKVKGLTSTKTQLPL 60

Query: 86  SHYSLE-----KREDDAVDIGEILLGE---------------CCGL-------------- 145
           S+YSL      K E+DA ++GEILLG+                C +              
Sbjct: 61  SYYSLPFCRPGKIEEDAENLGEILLGDRSENSPYVAVLMEHKLCNIICRIKIDDKGAKEL 120

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 121 KEKIEDEYMIHMTLDNLPLVHPIRIFEHESPLTYQLGFHIGLKGYYPEETFGKYFIYNHL 180

Query: 206 ------------------------------------------------------------ 265
                                                                       
Sbjct: 181 SFTIKYYQDTRTNSTRIVGFEAKPFRFXXXXXXXXXEKNTRLSTCDPSSKVTVLNSDGPQ 240

Query: 266 -----------------------AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVI 282
                                  A RWDAY ATRDD+  WFSIMNGLESIL+ISGILAVI
Sbjct: 241 EVEEGNEIIFTYDVEYQESDVDWASRWDAYLATRDDQMHWFSIMNGLESILVISGILAVI 300

BLAST of Spg016831 vs. ExPASy TrEMBL
Match: A0A5A7VGE1 (Transmembrane 9 superfamily member OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003010 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.5e-39
Identity = 165/511 (32.29%), Postives = 191/511 (37.38%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASR S  V TLTI+L+FLLL H+ HCFN FG+ PVD +KGD LKVKVKGLTSTKTQLP+
Sbjct: 1   MASRISLPVQTLTIALSFLLLFHSVHCFNLFGIRPVDFKKGDDLKVKVKGLTSTKTQLPV 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCG--------------- 145
           S+YSL     EK EDDA ++GEILLG               + C                
Sbjct: 61  SYYSLPFCRPEKIEDDAENLGEILLGDRSENSPYVAKMLEHQLCNIVCRIELDGKGAEEL 120

Query: 146 -------------------------------LAF-------------------------- 205
                                          LAF                          
Sbjct: 121 KEKIEDEYMVHMILDNLPLVHPIRIFEHDSPLAFQLGFHMGLKGYYPEEQAKYFIYNHLL 180

Query: 206 ------------------------------------------------------------ 265
                                                                       
Sbjct: 181 FTIKYYHDIQSNSTRIVGFEVKPFSIKHEYNGKWKERNTRLSTCDPIRKIVVMNSDGPQM 240

Query: 266 ------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVIV 284
                                   RWDAY ATRDD+  WFSI+NGLESIL+ISGILAVIV
Sbjct: 241 VEEGKEIIFTYDIEFQESDVDWPSRWDAYLATRDDQMHWFSILNGLESILVISGILAVIV 300

BLAST of Spg016831 vs. ExPASy TrEMBL
Match: A0A1S3BU94 (Transmembrane 9 superfamily member OS=Cucumis melo OX=3656 GN=LOC103493745 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.5e-39
Identity = 165/511 (32.29%), Postives = 191/511 (37.38%), Query Frame = 0

Query: 26  MASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPI 85
           MASR S  V TLTI+L+FLLL H+ HCFN FG+ PVD +KGD LKVKVKGLTSTKTQLP+
Sbjct: 1   MASRISLPVQTLTIALSFLLLFHSVHCFNLFGIRPVDFKKGDDLKVKVKGLTSTKTQLPV 60

Query: 86  SHYSL-----EKREDDAVDIGEILLG---------------ECCG--------------- 145
           S+YSL     EK EDDA ++GEILLG               + C                
Sbjct: 61  SYYSLPFCRPEKIEDDAENLGEILLGDRSENSPYVAKMLEHQLCNIVCRIELDGKGAEEL 120

Query: 146 -------------------------------LAF-------------------------- 205
                                          LAF                          
Sbjct: 121 KEKIEDEYMVHMILDNLPLVHPIRIFEHDSPLAFQLGFHMGLKGYYPEEQAKYFIYNHLL 180

Query: 206 ------------------------------------------------------------ 265
                                                                       
Sbjct: 181 FTIKYYHDIQSNSTRIVGFEVKPFSIKHEYNGKWKERNTRLSTCDPIRKIVVMNSDGPQM 240

Query: 266 ------------------------RWDAYHATRDDETRWFSIMNGLESILLISGILAVIV 284
                                   RWDAY ATRDD+  WFSI+NGLESIL+ISGILAVIV
Sbjct: 241 VEEGKEIIFTYDIEFQESDVDWPSRWDAYLATRDDQMHWFSILNGLESILVISGILAVIV 300

BLAST of Spg016831 vs. TAIR 10
Match: AT5G25100.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 100.1 bits (248), Expect = 3.0e-21
Identity = 83/245 (33.88%), Postives = 112/245 (45.71%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVI--------VGRFTVIFI------ 171
           A RWDAY    D++  WFSI+N L  +L +SG++A+I        + R+  +        
Sbjct: 266 ASRWDAYLLMSDNQIHWFSIVNSLMIVLFLSGMVAMIMLRTLYRDISRYNELETQEEAQE 325

Query: 172 ----------MVQPLSNADL----------------------ELSFKS--------TTML 231
                     + +P +N+DL                       L F S        T ML
Sbjct: 326 ETGWKLVHGDVFRPPANSDLLCVYVGTGVQCLGMVLVTMIFAMLGFLSPSNRGGLMTAML 385

Query: 232 WLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKSY- 284
            LW+FM L  GY S+R YKMF  T WK+I+ +  AF FP V+  IFF+LNAL+WG KS  
Sbjct: 386 LLWVFMGLFAGYASSRLYKMFKGTEWKRIAFR-TAFLFPAVVSAIFFVLNALIWGQKSSG 445

HSP 2 Score: 68.9 bits (167), Expect = 7.4e-12
Identity = 38/73 (52.05%), Postives = 46/73 (63.01%), Query Frame = 0

Query: 40  SLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPISHYSL-----EKRE 99
           S+  LL +H AH F   GV P D EKGD LKVKV  LTS KTQLP S+YSL     +K  
Sbjct: 15  SVILLLSIHVAHSFYLPGVAPQDFEKGDELKVKVNKLTSIKTQLPYSYYSLPFCRPKKIV 74

Query: 100 DDAVDIGEILLGE 108
           D   ++GE+L G+
Sbjct: 75  DSTENLGEVLRGD 87

BLAST of Spg016831 vs. TAIR 10
Match: AT5G25100.2 (Endomembrane protein 70 protein family )

HSP 1 Score: 100.1 bits (248), Expect = 3.0e-21
Identity = 83/245 (33.88%), Postives = 112/245 (45.71%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVI--------VGRFTVIFI------ 171
           A RWDAY    D++  WFSI+N L  +L +SG++A+I        + R+  +        
Sbjct: 273 ASRWDAYLLMSDNQIHWFSIVNSLMIVLFLSGMVAMIMLRTLYRDISRYNELETQEEAQE 332

Query: 172 ----------MVQPLSNADL----------------------ELSFKS--------TTML 231
                     + +P +N+DL                       L F S        T ML
Sbjct: 333 ETGWKLVHGDVFRPPANSDLLCVYVGTGVQCLGMVLVTMIFAMLGFLSPSNRGGLMTAML 392

Query: 232 WLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKSY- 284
            LW+FM L  GY S+R YKMF  T WK+I+ +  AF FP V+  IFF+LNAL+WG KS  
Sbjct: 393 LLWVFMGLFAGYASSRLYKMFKGTEWKRIAFR-TAFLFPAVVSAIFFVLNALIWGQKSSG 452

HSP 2 Score: 62.0 bits (149), Expect = 9.0e-10
Identity = 38/80 (47.50%), Postives = 46/80 (57.50%), Query Frame = 0

Query: 40  SLNFLLLVHAAHCFNFFGVHPVDSEK-------GDGLKVKVKGLTSTKTQLPISHYSL-- 99
           S+  LL +H AH F   GV P D EK       GD LKVKV  LTS KTQLP S+YSL  
Sbjct: 15  SVILLLSIHVAHSFYLPGVAPQDFEKDSISEAIGDELKVKVNKLTSIKTQLPYSYYSLPF 74

Query: 100 ---EKREDDAVDIGEILLGE 108
              +K  D   ++GE+L G+
Sbjct: 75  CRPKKIVDSTENLGEVLRGD 94

BLAST of Spg016831 vs. TAIR 10
Match: AT5G10840.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 93.6 bits (231), Expect = 2.8e-19
Identity = 79/247 (31.98%), Postives = 109/247 (44.13%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVIVGR-------------------- 171
           A RWD Y    D++  WFSI+N L  +L +SG++A+I+ R                    
Sbjct: 270 ASRWDTYLLMSDNQIHWFSIVNSLMIVLFLSGMVAMIMLRTLYRDISRYNELETQEEAQE 329

Query: 172 ------------------------------------FTVIFIMVQPLSNADLELSFKSTT 231
                                                T+IF M+  LS ++       T 
Sbjct: 330 ETGWKLVHGDVFRLPTNSDLLCVYVGTGVQCLGMVFVTMIFAMLGFLSPSN--RGGLMTA 389

Query: 232 MLWLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKS 284
           ML LW+FM L  GY S+R YKMF  T WK+I+ +  AF FP V+  IFF+LNAL+WG KS
Sbjct: 390 MLLLWVFMGLFAGYASSRLYKMFKGTEWKRIAFR-TAFLFPAVVSAIFFVLNALIWGQKS 449

HSP 2 Score: 73.2 bits (178), Expect = 3.9e-13
Identity = 42/86 (48.84%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 27  ASRSSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPIS 86
           +SR   +     I+L FLL +H AH F   GV P D EKGD LKVKV  LTS KTQLP S
Sbjct: 8   SSRRILESSGCAIALIFLLFIHGAHSFYLPGVAPQDFEKGDELKVKVNKLTSIKTQLPYS 67

Query: 87  HYSL-----EKREDDAVDIGEILLGE 108
           +YSL      K  D   ++GE+L G+
Sbjct: 68  YYSLPFCRPSKIVDSTENLGEVLRGD 93

BLAST of Spg016831 vs. TAIR 10
Match: AT1G55130.1 (Endomembrane protein 70 protein family )

HSP 1 Score: 87.4 bits (215), Expect = 2.0e-17
Identity = 73/247 (29.55%), Postives = 108/247 (43.72%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVIVGR-------------------- 171
           A RWD Y    DD+  WFSI+N L  +L +SG++A+I+ R                    
Sbjct: 259 ASRWDTYLLMNDDQIHWFSIINSLMIVLFLSGMVAMIMMRTLYKDISNYNQLETQDEAQE 318

Query: 172 ------------------------------------FTVIFIMVQPLSNADLELSFKSTT 231
                                                T+IF ++  LS ++      +T 
Sbjct: 319 ETGWKLVHGDVFRTPMNSGLLCVYVGTGVQIFGMTLVTMIFALLGFLSPSN--RGGLTTA 378

Query: 232 MLWLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKS 284
           M+ LW+FM +  GY S+R +KMF    WK+I+LK  AF FP +++ IFF+LN L+WG +S
Sbjct: 379 MVLLWVFMGIFAGYSSSRLHKMFKGNEWKRITLK-TAFMFPGILFAIFFVLNTLIWGERS 438

HSP 2 Score: 50.8 bits (120), Expect = 2.1e-06
Identity = 32/75 (42.67%), Postives = 41/75 (54.67%), Query Frame = 0

Query: 38  TISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPISHYSLE-----K 97
           T+ L+FL      H F   GV P D +KGD L VKV  L+STKTQLP   Y L      K
Sbjct: 10  TLLLSFLFF-STLHAFYLPGVAPRDFQKGDPLYVKVNKLSSTKTQLPYDFYYLNYCKPPK 69

Query: 98  REDDAVDIGEILLGE 108
             +   ++GE+L G+
Sbjct: 70  ILNTGENLGEVLRGD 83

BLAST of Spg016831 vs. TAIR 10
Match: AT3G13772.1 (transmembrane nine 7 )

HSP 1 Score: 85.5 bits (210), Expect = 7.6e-17
Identity = 78/245 (31.84%), Postives = 109/245 (44.49%), Query Frame = 0

Query: 112 AFRWDAYHATRDDETRWFSIMNGLESILLISGILAVIVGRFTVIFI-------------- 171
           A RWD Y    DD+  WFSI+N L  +L +SG++A+I+ R     I              
Sbjct: 263 ASRWDTYLLMNDDQIHWFSIINSLMIVLFLSGMVAMIMMRTLYKDISNYNQLETQDEAQE 322

Query: 172 ----------MVQPLSNADL----------------------ELSFKS--------TTML 231
                     + +P  N+ L                       L F S        T M+
Sbjct: 323 ETGWKLVHGDVFRPPVNSGLLCVYVGTGVQIFGMSLVTMMFALLGFLSPSNRGGLMTAMV 382

Query: 232 WLWIFMRLCVGYVSARFYKMFNTTFWKKISLKMMAFTFPIVIYVIFFMLNALLWGHKSY- 284
            LW+FM +  GY S+R +KMF    WK+++LK  AF FP +++ IFF+LNAL+WG +S  
Sbjct: 383 LLWVFMGIFAGYSSSRLHKMFKGNKWKRMTLK-TAFMFPGILFAIFFVLNALIWGEQSSG 442

HSP 2 Score: 52.4 bits (124), Expect = 7.1e-07
Identity = 35/83 (42.17%), Postives = 49/83 (59.04%), Query Frame = 0

Query: 30  SSFQVWTLTISLNFLLLVHAAHCFNFFGVHPVDSEKGDGLKVKVKGLTSTKTQLPISHYS 89
           SSF+ +  T+ L+FL     +  F   GV P D +KGD L VKV  L+STKTQLP  +Y 
Sbjct: 7   SSFRFYA-TLLLSFLSF-SLSRAFYLPGVAPRDFQKGDPLYVKVNKLSSTKTQLPYDYYY 66

Query: 90  LE-----KREDDAVDIGEILLGE 108
           L      K  ++A ++GE+L G+
Sbjct: 67  LNYCKPPKILNNAENLGEVLRGD 87