Spg016826 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016826
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold9: 39112182 .. 39116793 (+)
RNA-Seq Expression	Spg016826
Synteny	Spg016826

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTTGGTCGCCGAGTCGTCCACTTCCATGGAAGATTCTAAAAACTCCATAAATATGTCAGCAGCGCCATTGAAGCTCTCAAACAAAAAATCAAAATCAGTTTGCTGATCTTCACCTTTCTCACGCTGAAATCAGAATCCGATCAATCGGAAAATCAAACACATGGCGGAGAAGAACAACACATCATCCAGACTCGGCAGCTCGATTCTGGTACCTTGCGTTCAAGAGTTGGCCAAGAAGCCGATCATCGAAATTCCTCCTCGATACGTCCGCCCGGATCTCGGCCACATCCCGATCGTCGTCTCCGCCGCTTCCGAAATCCCCGTTATCGACATGTCGAGCTTGCTTTCCGACGCTTCAATGGAGTCGGAGTTAAATCGGCTCGATTCCGCCTGCAAAAATTGGGGATTCTTCCAGGTTTGTCTGTGTCTCTGTGTTTCTCTGTTTGCATGAATCTGAATTGGCCTCGGATGGTGAAATCTAGTCGACGGTGCAGTTGGTGAACCATGGAGTGAGCGGTTCGCTGATCGAGAAAATGAAGGCGGAAACGCAGAAATTCTTCGATCTGGCGATTGAAGAGAAGAAAAAGCTATGGCAGAGAGAGGGAGATGTAGAAGGATTTGGGCAAGTTTTCGTAGTATCTGAAGAACAGAAACTGGATTGGTGTGACATTTTCTTCCTTACTACTGCGCCTCCACATCTCAGGAAACCGCGCTTATTTCAAAACCTCCCACTCGCCTTCCGGTTCTCTCTTTCTCTCTTTCACTCTCAAACTCGTTGCATTACAAACTTTAGTTCGTTAATTGCTTTGGTAAATTTCAATTTAGTCCATATAATTTTTGAATTTTTTTTTTTGTTTTTTTCTTTTTAAAATTAAATTTGTTTTCTCATAATTTTTTTTTTATAATAGTTTTATAATAGTTTTCAAGTTTGTTAGAGAATTTGAATTCTAGGTCAAATTCTAAAAATAAAAACTAGTTTTTTTTAGTTTTCAAAACTTGGCTTGGTTTTTTAAAATATGGAAGAAGTGGATAACAGGATAGAAACTTAGCTTATAGGTAGTCAAAGCGAACATAGTTCAACAGTAATTGACATTTTGATGATGAGGTTAGAGGTTCGAATCTGCGCACCCCTGGATTTTGATTTTTGAAAATTAGGTTCGGTAAAATAATAATAATAAAAAAAAAATTTTAGAAAAGGGGATTGTTAACCAAAGTTGTGGCTAAAATTGTTAACCAAAGAAAAAGGGGATTGTAACATGCCACTTGAAAAAAATAAAGTTTATAGAAAGGTGGGTAGGATTTTCCGAAGGACATACTAGATTTTAGGAATACACTTGTCCAAAGTGTTAATAAAAGAAGAAAAAGACAATTGAGTTATCCATCAAATTTTTATAAACAAATTTTATATGGAATGATGTAATGGATAATCTTTAATACATCAAGAAAAAATATCGAAAATTTTATAAGATTAAATAGAAAATAGAAAATACTTAAAAGGTTAGGAACTATTTTGAATTTCTTCTAAAAAAAAAAAAACTATTTTGAATATTTTACCATCCAAAAGCTTTAATTTATTCTCTCTATAAATTTTTTACCTCTCAGTTCAACTATGCTTATGTTGATTACTCTCTATAATTGGGTTTAGTTCCAATTTTGTTAGATCTCACAAAATCATTCGTAACCAATCTTGTTTACCCGCTTGAAAATTTACTCGTTTTACTTTTTTTTCTATTTTTTTGACTCTATTTTAAACATCAATACAATCAACTAATCCATCAACCAAATGACATTGCCTCTAAATAAAATAGCTTATCTTCAATTTGAGTATAGTTCAATGGATAAAATATTTAATTATCATTACTTAAGAATGATGGTCTGATCTCTACCCTCACAATTGTTAAATAAAATAATAATAATAAATTACAAAATGTTGGACAAAATACACTTTTGGTTGGAGCTATAGTGTCTCAAGTTCAAAAAAAACATTTTTTAATCCTTGACGTTTGAGAAGTTGTTCTAAATGTCATAAACCCTAAAATTTTTTGGTACTCCAAGAGAACCCATACTCCTTTTTTTTTAAACATCCATACTCCGATCCCTTTGTTTAATCTAAAATTTTGGCTTTCTAAATATTTCTTTAATGCTAGCTACTAGCTAGCACATCAGTTTTTAAAGGATGTAGAGATGTGACAACTAATAAATGGGATGTGGTACGAAATATTCCACCATATCATGATATGGTCGTTAGATTTTTTTTTTAATTATTATTTTTTTTTTGACATGGTTGTTAGATTTTTTTTAAAAAAAAATTTTTGACACGGTCATTAGATTGTTGAGTGTACACTTTTGCAAAATATATTAGTTTATTATTATTTTTATTTTTTTAAAAATTTCTCTAAGAACTATTGTAGTAGATAATCAATGCAGTTTAAACCCTGTTTGATAATCATTTTGTTTTTTATTTTTGAAAATTATGCTTGTTTTCTCTCAATTTCCTTACCATAATTTTCATTTTTGTTAAGTAGACACTTGAATTCTTAGTCAAATTCCAAAAATAAAAATAAAACTACGTTTTTTTGTTTTAAAATTTGACTTGATTTTTAAAAACATGAAAAGTTGTAAATAACAAATCAAAGAAAGTTTGGGGGAATATAGTGTTTATAAGCTTAATTTTAAAAAATCAAAAACAAAAAACAAAATAGTTATCAAACCGGGCCTTAGTATTCGGAGTTGAATTTTCCGATTCACGTGCAGAAACACATTGGAGGAGTACTCGGCGGTGGTGAAGCATCTCGCGGCGGCGATTTTCGAGCAATTTGAAAAAGCGTTGGGGATTAAGGATGGGGAAATCACAGAGATCTTCAAGGACGGAAATCAGTCGATGAGGATGAACTATTACCCGCCGTGCCCGTCGCCGGAGAAGGTGATCGGACTGACTCCTCATTCCGACGCCGTCGGCCTCACAATTCTGCTGCAGATCAACGAAGTGGAAGGCCTGAAGATTAAGAAGGACGGGAACTGGGTTTCTGTTACGCCGCTTCCAAATGCGTTCGTCGTCAACATTGGCGACATCATGGAGGTAACTTTTCAATTTTCAGTAACATGAAATTCCCAAATTTGGAGATTTGATTTAGGGCTCTGTTAAGATAGAAAACAACTTTCCACCTTGACGCGATAGATACCACTTTTCGATTAAATCACAATCAACGAGTTCAAATTTTATTTTAGATTAAATTAAAATTTCTAATTTCTTAGAGAGATTTTTTCCCCTTTACTAATGACTAAGAGATTTGTGATTTAAATCTTCTATCTCTAAATATTGTCAAAATAATAATAATAATAATAACAACTTATCTCACTAATAATGGAAGTCGACTCAATCTAACTTTGAAAGTTCAAAATCTATTATCATATACTTTTTTAAAGACGAAGAATCTAACTTTAATTTATTTTTTTGTTTCTCTATCAAAAAAAAAAATGTTTTTATACTTACAAATGTCTTTATATATATATATAAGTATTTTGGTCATGGTCATAATTTCTTTTTAAGAAAGGTCATCGTCATAATTCTATCATATATGTGTGTTGTCATTGGTTGGTGAGCTAAATATTAAGTAAAATGGAGACTTAAATTCATGCCACTCAATACTTAATATCCACCTCAATTAATCTACTCCATAAATTAAATTGTTTTATTTTTATTGGACTAATAATATTGATAAAATTTGTCAACTCGAGTGTAGTTGAGCTAATTCGAAAAAAAATATATATATATAGATTCTCAATCAAGAAGTTAGAAATTCAAATCTCCCACCCTCACATGTTAATTAAAAAAAGTCCCTACATATCCCATTAAAATCTTGACAACTAAGAAAGAGGGAGGGAGAAAATGAGAAAATTTTTTAAAAAAATTTTATCTCTCCTAATTGTCAACATTTTAATGGTGGTAGATCGGTATGCAGCGAAGTTTTTTACTTAAAAAAAATATGATGGAATTTTCTTAATGAGGAGTTTATCCTGAGGCATTTTTTTGTTACCTAAAATGTTGGGTAATAATAATCTTTAACTACTACTCTAACAAATATGATAGTGAGTTGGTTTTGTTTTGTTTGATGCATATATATTTGTATGTGTGGCAGATGGTAACAAATGGGAAATACAGAAGCATAGAGCACTGCGCAACGGTGAATTCAGAGAGTGAAAGATTGTCCATAGCAACCTTCCACAATCCAAGTTCTGAGAAAGAAATAAGCTCTGCACCATCCCTTATCACACCTCATTCTCCTCCTTTGTTTAGAACCTTGACAACACAAGAGTATGCGAGGGGCCTCTTTGCTCGCAAACTAGACGGAAAATCTTACCGCGATGCCATGAGAATTCACCACCCACCTCCTACCTCATCTCTTTCCCAATCAAACTCTCTAAGTTTGAATTAGAGATCATGTTTGTCTGTTTGGATACAATCTCTTTCTTCATGTCATATCAACACTCATTATCTTCCTACCTAATAAATCCAAACTAGAGACTAAAGTTGAATAATAAATTTGGATATGTTAGTTCCTAAAGTTTCAAATGAATGATAATAATGCATGTTGCAATATTATTCTAAAC

mRNA sequence

ATGGCGGAGAAGAACAACACATCATCCAGACTCGGCAGCTCGATTCTGGTACCTTGCGTTCAAGAGTTGGCCAAGAAGCCGATCATCGAAATTCCTCCTCGATACGTCCGCCCGGATCTCGGCCACATCCCGATCGTCGTCTCCGCCGCTTCCGAAATCCCCGTTATCGACATGTCGAGCTTGCTTTCCGACGCTTCAATGGAGTCGGAGTTAAATCGGCTCGATTCCGCCTGCAAAAATTGGGGATTCTTCCAGTCGACGGTGCAGTTGGTGAACCATGGAGTGAGCGGTTCGCTGATCGAGAAAATGAAGGCGGAAACGCAGAAATTCTTCGATCTGGCGATTGAAGAGAAGAAAAAGCTATGGCAGAGAGAGGGAGATGTAGAAGGATTTGGGCAAGTTTTCGTAGTATCTGAAGAACAGAAACTGGATTGGTGTGACATTTTCTTCCTTACTACTGCGCCTCCACATCTCAGGAAACCGCGCTTATTTCAAAACCTCCCACTCGCCTTCCGAAACACATTGGAGGAGTACTCGGCGGTGGTGAAGCATCTCGCGGCGGCGATTTTCGAGCAATTTGAAAAAGCGTTGGGGATTAAGGATGGGGAAATCACAGAGATCTTCAAGGACGGAAATCAGTCGATGAGGATGAACTATTACCCGCCGTGCCCGTCGCCGGAGAAGGTGATCGGACTGACTCCTCATTCCGACGCCGTCGGCCTCACAATTCTGCTGCAGATCAACGAAGTGGAAGGCCTGAAGATTAAGAAGGACGGGAACTGGGTTTCTGTTACGCCGCTTCCAAATGCGTTCGTCGTCAACATTGGCGACATCATGGAGATGGTAACAAATGGGAAATACAGAAGCATAGAGCACTGCGCAACGGTGAATTCAGAGAGTGAAAGATTGTCCATAGCAACCTTCCACAATCCAAGTTCTGAGAAAGAAATAAGCTCTGCACCATCCCTTATCACACCTCATTCTCCTCCTTTGTTTAGAACCTTGACAACACAAGAGTATGCGAGGGGCCTCTTTGCTCGCAAACTAGACGGAAAATCTTACCGCGATGCCATGAGAATTCACCACCCACCTCCTACCTCATCTCTTTCCCAATCAAACTCTCTAAGTTTGAATTAG

Coding sequence (CDS)

ATGGCGGAGAAGAACAACACATCATCCAGACTCGGCAGCTCGATTCTGGTACCTTGCGTTCAAGAGTTGGCCAAGAAGCCGATCATCGAAATTCCTCCTCGATACGTCCGCCCGGATCTCGGCCACATCCCGATCGTCGTCTCCGCCGCTTCCGAAATCCCCGTTATCGACATGTCGAGCTTGCTTTCCGACGCTTCAATGGAGTCGGAGTTAAATCGGCTCGATTCCGCCTGCAAAAATTGGGGATTCTTCCAGTCGACGGTGCAGTTGGTGAACCATGGAGTGAGCGGTTCGCTGATCGAGAAAATGAAGGCGGAAACGCAGAAATTCTTCGATCTGGCGATTGAAGAGAAGAAAAAGCTATGGCAGAGAGAGGGAGATGTAGAAGGATTTGGGCAAGTTTTCGTAGTATCTGAAGAACAGAAACTGGATTGGTGTGACATTTTCTTCCTTACTACTGCGCCTCCACATCTCAGGAAACCGCGCTTATTTCAAAACCTCCCACTCGCCTTCCGAAACACATTGGAGGAGTACTCGGCGGTGGTGAAGCATCTCGCGGCGGCGATTTTCGAGCAATTTGAAAAAGCGTTGGGGATTAAGGATGGGGAAATCACAGAGATCTTCAAGGACGGAAATCAGTCGATGAGGATGAACTATTACCCGCCGTGCCCGTCGCCGGAGAAGGTGATCGGACTGACTCCTCATTCCGACGCCGTCGGCCTCACAATTCTGCTGCAGATCAACGAAGTGGAAGGCCTGAAGATTAAGAAGGACGGGAACTGGGTTTCTGTTACGCCGCTTCCAAATGCGTTCGTCGTCAACATTGGCGACATCATGGAGATGGTAACAAATGGGAAATACAGAAGCATAGAGCACTGCGCAACGGTGAATTCAGAGAGTGAAAGATTGTCCATAGCAACCTTCCACAATCCAAGTTCTGAGAAAGAAATAAGCTCTGCACCATCCCTTATCACACCTCATTCTCCTCCTTTGTTTAGAACCTTGACAACACAAGAGTATGCGAGGGGCCTCTTTGCTCGCAAACTAGACGGAAAATCTTACCGCGATGCCATGAGAATTCACCACCCACCTCCTACCTCATCTCTTTCCCAATCAAACTCTCTAAGTTTGAATTAG

Protein sequence

MAEKNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSSLLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRIHHPPPTSSLSQSNSLSLN

Homology

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BLAST of Spg016826 vs. NCBI nr
Match: KAG6591778.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 589.0 bits (1517), Expect = 3.0e-164
Identity = 297/365 (81.37%), Postives = 324/365 (88.77%), Query Frame = 0


Query: 1   MAEKNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSS 60
           MAEKNNT SR G SILVP VQELAK+PIIE+P RY+RPDL   PI VSAASEIPVIDMS 
Sbjct: 1   MAEKNNT-SRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPI-VSAASEIPVIDMSK 60

Query: 61  LLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKK 120
             SDASM SEL RL SACKNWGFF    QLVNHGVS SLIEKMKAETQKFF+L IEEKKK
Sbjct: 61  FHSDASMHSELARLHSACKNWGFF----QLVNHGVSDSLIEKMKAETQKFFNLPIEEKKK 120

Query: 121 -LWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYS 180
            LWQ E D EGFGQ FVVSEEQKLDWCD+FF+ TAPPHLRKPRLFQNLPLAFRNT+EEYS
Sbjct: 121 RLWQSEEDAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYS 180

Query: 181 AVVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAV 240
           A VK+LA AIFEQFEKALGIK+GE+TEIFKDG+QSMRMNYYPPCP PEKVIGLTPHSDAV
Sbjct: 181 AEVKNLAMAIFEQFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAV 240

Query: 241 GLTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSE 300
           GLTILLQINE+EGLKIKKDGNWV+VTPLPNAF+VNIGDIME++TNG+Y+SIEHCATVN++
Sbjct: 241 GLTILLQINEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAK 300

Query: 301 SERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMR 360
            ERLSIATF+NPS EKEI + PSLITPHSPPLF TLT Q++ R LF+RKLDGKS+ D +R
Sbjct: 301 RERLSIATFNNPSLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLR 359

Query: 361 IHHPP 365
           IH PP
Sbjct: 361 IHTPP 359

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BLAST of Spg016826 vs. NCBI nr
Match: KAG7024656.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 586.6 bits (1511), Expect = 1.5e-163
Identity = 296/365 (81.10%), Postives = 323/365 (88.49%), Query Frame = 0


Query: 1   MAEKNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSS 60
           MAEKNNT SR G SILVP VQELAK+PIIE+P RY+RPDL   PI VSAASEIPVIDMS 
Sbjct: 348 MAEKNNT-SRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPI-VSAASEIPVIDMSK 407

Query: 61  LLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKK 120
             SDASM SEL RL SACKNWGFF    QLVNHGVS SLIEKMKAETQKFF+L IEEKKK
Sbjct: 408 FHSDASMHSELARLHSACKNWGFF----QLVNHGVSDSLIEKMKAETQKFFNLPIEEKKK 467

Query: 121 -LWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYS 180
            LWQ E D EGFGQ FVVSEEQKLDWCD+FF+ TAPPHLRKPRLFQNLPLAFRNT+EEYS
Sbjct: 468 RLWQSEEDAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYS 527

Query: 181 AVVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAV 240
           A VK+LA AIFEQFEKALGIK+GE+TEIFKDG+QSMR NYYPPCP PEKVIGLTPHSDAV
Sbjct: 528 AEVKNLAMAIFEQFEKALGIKEGELTEIFKDGSQSMRTNYYPPCPEPEKVIGLTPHSDAV 587

Query: 241 GLTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSE 300
           GLTILLQINE+EGLKIKKDGNWV+VTPLPNAF+VNIGDIME++TNG+Y+SIEHCATVN++
Sbjct: 588 GLTILLQINEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAK 647

Query: 301 SERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMR 360
            ERLSIATF+NPS EKEI + PSLITPHSPPLF TLT Q++ R LF+RKLDGKS+ D +R
Sbjct: 648 RERLSIATFNNPSLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLR 706

Query: 361 IHHPP 365
           IH PP
Sbjct: 708 IHTPP 706

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BLAST of Spg016826 vs. NCBI nr
Match: XP_022935942.1 (protein SRG1-like [Cucurbita moschata])

HSP 1 Score: 585.1 bits (1507), Expect = 4.3e-163
Identity = 295/365 (80.82%), Postives = 323/365 (88.49%), Query Frame = 0


Query: 1   MAEKNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSS 60
           MAEKNNT SR G SILVP VQELAK+PIIE+P RY+RPDL   PI VSAASEIPVIDMS+
Sbjct: 1   MAEKNNT-SRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPI-VSAASEIPVIDMSN 60

Query: 61  LLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKK 120
             SDASM SEL RL SACKNWGFF    QLVNHGVS SLIEKMKAETQ FF+L IEEKKK
Sbjct: 61  FHSDASMHSELARLHSACKNWGFF----QLVNHGVSDSLIEKMKAETQMFFNLPIEEKKK 120

Query: 121 -LWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYS 180
            LWQ E D EGFGQ FVVSEEQKLDWCD+FF+ TAPPHLRKPRLFQNLPLAFRNT+EEYS
Sbjct: 121 RLWQSEEDAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYS 180

Query: 181 AVVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAV 240
           A VK+LA AIFEQFEKALGIK+GE+TEIFKDG+QSMRMNYYPPCP PEKVIGLTPHSDAV
Sbjct: 181 AEVKNLAMAIFEQFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAV 240

Query: 241 GLTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSE 300
           GLTILLQINE+EGLKIKKDGNWV+VTPLPNAF+VNIGDIME++TNG+Y+SIEHCATVN++
Sbjct: 241 GLTILLQINEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAK 300

Query: 301 SERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMR 360
            ERLSIATF+NP  EKEI + PSLITPHSPPLF TLT Q++ R LF+RKLDGKS+ D +R
Sbjct: 301 RERLSIATFNNPRLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLR 359

Query: 361 IHHPP 365
           IH PP
Sbjct: 361 IHTPP 359

[Close]

BLAST of Spg016826 vs. NCBI nr
Match: XP_023534939.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578.9 bits (1491), Expect = 3.1e-161
Identity = 288/360 (80.00%), Postives = 318/360 (88.33%), Query Frame = 0


Query: 1   MAEKNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSS 60
           MAEKNN +SR G SILVP VQELAK+PI+E+P RY+RPDL   PI VSAASEIPVIDMS+
Sbjct: 1   MAEKNNNTSRFGGSILVPSVQELAKQPIVEVPSRYIRPDLHSFPI-VSAASEIPVIDMSN 60

Query: 61  LLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKK 120
             S+ASM SEL RL SACKNWGFF    QLVNHGVS  L+EKMKAETQ FF+L IEEKKK
Sbjct: 61  FHSNASMYSELARLHSACKNWGFF----QLVNHGVSDLLMEKMKAETQMFFNLPIEEKKK 120

Query: 121 LWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSA 180
           LWQ E D EGFGQ FVVSEEQKLDWCD+FF+ TAPPHLRKPRLFQNLPLAFRNT+EEYSA
Sbjct: 121 LWQSEEDAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYSA 180

Query: 181 VVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVG 240
            VK+LA AIFEQFEKALGIKDGE+TEIFKDG+QSMRMNYYPPCP PEKVIGLTPHSDAVG
Sbjct: 181 EVKNLAIAIFEQFEKALGIKDGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAVG 240

Query: 241 LTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSES 300
           LTILLQINE+EGLKIKKDGNWV+VTPLPNAF+VNIGDIME+ TNG+Y+SIEHCATVN++ 
Sbjct: 241 LTILLQINEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEITTNGEYKSIEHCATVNAKR 300

Query: 301 ERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 360
           ERLSIATF+NPS EKEI   PSL+TPHSPPLF TLT Q++ R LF+RKLDGKS+ D +RI
Sbjct: 301 ERLSIATFNNPSLEKEIRPIPSLLTPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLRI 355

[Close]

BLAST of Spg016826 vs. NCBI nr
Match: XP_038897143.1 (protein SRG1-like [Benincasa hispida])

HSP 1 Score: 572.8 bits (1475), Expect = 2.2e-159
Identity = 288/365 (78.90%), Postives = 316/365 (86.58%), Query Frame = 0


Query: 1   MAEKNNT-SSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMS 60
           MAE+N+T +SR GSSILVP VQELAK+PI EIPPRY+RPDL H   + SAASEIPVIDMS
Sbjct: 1   MAEENSTNTSRYGSSILVPSVQELAKQPITEIPPRYIRPDLLHHVPIASAASEIPVIDMS 60

Query: 61  SLLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKK 120
           +  SDA+++SEL RL SACKNWGFF    QLVNHGVS SLIEKMK ET+K FDL IEEKK
Sbjct: 61  NFHSDATIDSELARLHSACKNWGFF----QLVNHGVSDSLIEKMKIETKKLFDLPIEEKK 120

Query: 121 KLWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYS 180
           KLWQREGDVEGFGQ FVVSEEQKLDW D+FF+ T+P HLRK RLFQNLPL FR TLE+YS
Sbjct: 121 KLWQREGDVEGFGQAFVVSEEQKLDWSDLFFIATSPSHLRKQRLFQNLPLTFRKTLEDYS 180

Query: 181 AVVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAV 240
           A VK LAAAIFE  EK LGIK GE+ E+FKDGNQSMRMNYYP CP PEK IGLTPHSDAV
Sbjct: 181 AEVKDLAAAIFEGVEKVLGIKKGELKEMFKDGNQSMRMNYYPRCPEPEKAIGLTPHSDAV 240

Query: 241 GLTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSE 300
           GLTILLQINEVEGLKIKKDGNW+ V PLPNAFVVN+GDIMEMVTNG+Y+SIEHCATVNS+
Sbjct: 241 GLTILLQINEVEGLKIKKDGNWIPVMPLPNAFVVNVGDIMEMVTNGEYKSIEHCATVNSK 300

Query: 301 SERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMR 360
           SERLSIATF+NPS EKEI   PSLITPHSPPLF+TLT QE+ +GLF +KLDGKSY DAMR
Sbjct: 301 SERLSIATFNNPSMEKEIRPIPSLITPHSPPLFKTLTYQEFVKGLFTQKLDGKSYLDAMR 360

Query: 361 IHHPP 365
           IH+ P
Sbjct: 361 IHYLP 361

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BLAST of Spg016826 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.1e-112
Identity = 205/353 (58.07%), Postives = 255/353 (72.24%), Query Frame = 0


Query: 13  SSILVPCVQELAK-KPIIEIPPRYVRPDLGHIPI--VVSAASEIPVIDMSSLLSDASMES 72
           SSILVP VQE+ K K I  +PPRYVR D     +        EIP+IDM  L S  +M+S
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 73  ELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVE 132
           E+ +LD ACK WGFF    QLVNHG+  S ++K+K+E Q FF+L +EEKKK WQR  ++E
Sbjct: 70  EVEKLDFACKEWGFF----QLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIE 129

Query: 133 GFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAI 192
           GFGQ FVVSE+QKLDW D+FF T  P  LRKP LF  LPL FR+TLE YS+ V+ +A  +
Sbjct: 130 GFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKIL 189

Query: 193 FEQFEKALGIKDGEITEIFKDGN--QSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQI 252
             +  +AL IK  E+ ++F D +  QSMRMNYYPPCP P++VIGLTPHSD+VGLT+L+Q+
Sbjct: 190 IAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQV 249

Query: 253 NEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIAT 312
           N+VEGL+IKKDG WV V PLPNAF+VNIGD++E++TNG YRSIEH   VNSE ERLSIAT
Sbjct: 250 NDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIAT 309

Query: 313 FHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           FHN    KE+  A SL+       F+ LT +EY  GLF+R LDGK+Y DA+RI
Sbjct: 310 FHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of Spg016826 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.1e-105
Identity = 198/361 (54.85%), Postives = 259/361 (71.75%), Query Frame = 0


Query: 10  RLGSSILVPCVQELAKKPIIEIPPRYV-RPDLGHIPI---VVSAASEIPVIDMSSLLSD- 69
           +LG+ + +P VQELAK  + EIP RY+   +   +P+   V+     +PVID+ +L+S  
Sbjct: 8   KLGNGMSIPSVQELAKLTLAEIPSRYICTVENLQLPVGASVIDDHETVPVIDIENLISSE 67

Query: 70  -ASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQ 129
             + + EL+RL SACK WGFF    Q+VNHGV  SL++ +K++ Q FF+L++ EK K  Q
Sbjct: 68  PVTEKLELDRLHSACKEWGFF----QVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQ 127

Query: 130 REGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVK 189
           ++GDVEGFGQ FV SE+Q LDW DIF + T P HLRKP LF  LPL  R T+E YS+ +K
Sbjct: 128 KDGDVEGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMK 187

Query: 190 HLAAAIFEQFEKAL---GIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVG 249
            L+  +FE+ EKAL    ++  EI+E+FKD  Q MRMNYYPPCP PE  IGLTPHSD  G
Sbjct: 188 KLSMVLFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGG 247

Query: 250 LTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSES 309
           LTILLQ+NEVEGL+IK +G W+SV PLPNAFVVN+GD++E++TNG YRS++H A VNS  
Sbjct: 248 LTILLQLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTK 307

Query: 310 ERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQ-EYARGLFARKLDGKSYRDAMR 361
           ERLSIATFH+P+ E EI    SLITP++P LFR+ +T  E      +RKLDGKS+ D+MR
Sbjct: 308 ERLSIATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMR 364

[Close]

BLAST of Spg016826 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.7e-101
Identity = 196/361 (54.29%), Postives = 253/361 (70.08%), Query Frame = 0


Query: 10  RLGSSILVPCVQELAKKPIIEIPPRYVRPD----LGHIPIVVSAASEIPVIDMSSLLSDA 69
           +LG+ + +P VQELAK  + EIP RYV  +    L     V++    IPVID+ +LLS  
Sbjct: 8   KLGNGMEIPSVQELAKLTLAEIPSRYVCANENLLLPMGASVINDHETIPVIDIENLLSPE 67

Query: 70  SM--ESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQ 129
            +  + EL+RL  ACK WGFF    Q+VNHGV  SL++ +K+E Q FF+L+++EK K  Q
Sbjct: 68  PIIGKLELDRLHFACKEWGFF----QVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKYEQ 127

Query: 130 REGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVK 189
            +GDVEGFGQ F+ SE+Q LDW DIF + T P HLRKP LF  LP+  R T+E YS+ +K
Sbjct: 128 EDGDVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETIESYSSEMK 187

Query: 190 HLAAAIFEQFEKALGIKDGEI---TEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVG 249
            L+  +F + EKAL ++  EI   +E+F DG Q+MRMNYYPPCP P   IGLT HSD  G
Sbjct: 188 KLSMVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDFGG 247

Query: 250 LTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSES 309
           LTILLQINEVEGL+IK++G W+SV PLPNAFVVN+GDI+E++TNG Y S++H A VNS +
Sbjct: 248 LTILLQINEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNSTN 307

Query: 310 ERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQ-EYARGLFARKLDGKSYRDAMR 361
           ERLSIATFH+PS E  I    SLITP +P LF++ +T  +       RKLDGKS+ D+MR
Sbjct: 308 ERLSIATFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEECKTRKLDGKSFLDSMR 364

[Close]

BLAST of Spg016826 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.0e-98
Identity = 183/357 (51.26%), Postives = 251/357 (70.31%), Query Frame = 0


Query: 10  RLGSSILVPCVQELAKKPIIEIPPRYV---RPDLGHIPIVVSAASEIPVIDMSSLLSDAS 69
           +LG+ + +P VQELAK  + EIP RY       L +I   V+    +PVID+ +LLS   
Sbjct: 8   KLGNGLSIPSVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEP 67

Query: 70  M--ESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQR 129
           +  + EL++L SACK WGFF    QLVNHGV   L++ +K+E + FF+L + EK K  Q+
Sbjct: 68  VVGKLELDKLHSACKEWGFF----QLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQ 127

Query: 130 EGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKH 189
           +GD EGFGQ ++ SE+Q+LDW ++F + + P HLRKP LF  LPL FR TLE Y + +K 
Sbjct: 128 DGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKK 187

Query: 190 LAAAIFEQFEKALGIKDGE-ITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTI 249
           L+  +FE  EK+L + + + +T++F+DG Q+MRMNYYPPCP PE V+GLT HSD  GLTI
Sbjct: 188 LSTVVFEMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTI 247

Query: 250 LLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERL 309
           LLQ+NEVEGL+I+K+  W+S+ PLP+AF+VN+GDI+E++TNG YRS+EH A VNS  ERL
Sbjct: 248 LLQLNEVEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERL 307

Query: 310 SIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           SIATFH+   E EI    SL+TP +P LF+    ++  +   +RKLDGKS+ D MR+
Sbjct: 308 SIATFHDSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

[Close]

BLAST of Spg016826 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.8e-72
Identity = 153/355 (43.10%), Postives = 216/355 (60.85%), Query Frame = 0


Query: 11  LGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIV-VSAASEIPVIDMSSLLSDASMES 70
           +G S+ V  VQ LA K +  +P RYVRP+L H  +V +  + EIPVID+S LL       
Sbjct: 8   VGGSLPVENVQVLAGKELKNLPNRYVRPELEHDDVVPIDNSLEIPVIDLSRLLDQQYACD 67

Query: 71  ELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVE 130
           EL +  SAC +WGFF    QL+NHGV   +IEKMK +T+ FF L  +EK    Q    +E
Sbjct: 68  ELAKFHSACLDWGFF----QLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGME 127

Query: 131 GFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAI 190
           G+GQ FV SEEQKLDW D+ FL T P   R  R +   P +FR T+E+YS  ++ +A  +
Sbjct: 128 GYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKVAMCL 187

Query: 191 FEQFEKALGIKDGEITE----IFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILL 250
                K LG++   +T+    +F   ++ +     P   S  + +GL+PHSDA GLT+L+
Sbjct: 188 TGMMAKNLGLESEILTKPLRTVFNREDELL-----PSMSSCGEGLGLSPHSDATGLTLLI 247

Query: 251 QINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSI 310
           Q+NEV GL IKKD  WV + P+  AFVVNIGD++E+++NG Y+SIEH A +N++ ERLSI
Sbjct: 248 QVNEVNGLHIKKDEKWVPIKPILGAFVVNIGDVIEIMSNGIYKSIEHRAVINTDKERLSI 307

Query: 311 ATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           A FH+P    +I   P L+  +    ++T+  ++Y       KLDGKS  D M++
Sbjct: 308 AAFHDPEYGTKIGPLPDLVKENGVK-YKTIDYEDYLIRSSNIKLDGKSLLDQMKL 352

[Close]

BLAST of Spg016826 vs. ExPASy TrEMBL
Match: A0A6J1F704 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111442702 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.1e-163
Identity = 295/365 (80.82%), Postives = 323/365 (88.49%), Query Frame = 0


Query: 1   MAEKNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSS 60
           MAEKNNT SR G SILVP VQELAK+PIIE+P RY+RPDL   PI VSAASEIPVIDMS+
Sbjct: 1   MAEKNNT-SRFGGSILVPSVQELAKQPIIEVPSRYIRPDLHSFPI-VSAASEIPVIDMSN 60

Query: 61  LLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKK 120
             SDASM SEL RL SACKNWGFF    QLVNHGVS SLIEKMKAETQ FF+L IEEKKK
Sbjct: 61  FHSDASMHSELARLHSACKNWGFF----QLVNHGVSDSLIEKMKAETQMFFNLPIEEKKK 120

Query: 121 -LWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYS 180
            LWQ E D EGFGQ FVVSEEQKLDWCD+FF+ TAPPHLRKPRLFQNLPLAFRNT+EEYS
Sbjct: 121 RLWQSEEDAEGFGQAFVVSEEQKLDWCDLFFIATAPPHLRKPRLFQNLPLAFRNTMEEYS 180

Query: 181 AVVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAV 240
           A VK+LA AIFEQFEKALGIK+GE+TEIFKDG+QSMRMNYYPPCP PEKVIGLTPHSDAV
Sbjct: 181 AEVKNLAMAIFEQFEKALGIKEGELTEIFKDGSQSMRMNYYPPCPEPEKVIGLTPHSDAV 240

Query: 241 GLTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSE 300
           GLTILLQINE+EGLKIKKDGNWV+VTPLPNAF+VNIGDIME++TNG+Y+SIEHCATVN++
Sbjct: 241 GLTILLQINEMEGLKIKKDGNWVAVTPLPNAFIVNIGDIMEIITNGEYKSIEHCATVNAK 300

Query: 301 SERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMR 360
            ERLSIATF+NP  EKEI + PSLITPHSPPLF TLT Q++ R LF+RKLDGKS+ D +R
Sbjct: 301 RERLSIATFNNPRLEKEIKAIPSLITPHSPPLFTTLTYQQFVRLLFSRKLDGKSHLDTLR 359

Query: 361 IHHPP 365
           IH PP
Sbjct: 361 IHTPP 359

[Close]

BLAST of Spg016826 vs. ExPASy TrEMBL
Match: A0A1S3CK11 (probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=LOC103501850 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 7.2e-156
Identity = 276/362 (76.24%), Postives = 309/362 (85.36%), Query Frame = 0


Query: 1   MAEKNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSS 60
           MAE+ N + R GSSILVP VQELAK PI EIP RY+ PDL H   + S AS+IPVIDMS+
Sbjct: 1   MAEEKNDTLRYGSSILVPSVQELAKHPITEIPHRYIHPDLLHHSPISSDASQIPVIDMSN 60

Query: 61  LLSDASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKK 120
           L S  +M+SEL+RL SACKNWGFF    QLV HGVS SL+E+MK ETQK F+L IEEKKK
Sbjct: 61  LRSHDTMDSELSRLHSACKNWGFF----QLVKHGVSDSLVERMKMETQKLFELPIEEKKK 120

Query: 121 LWQREGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSA 180
           LWQREGDVEGFGQ FV SEEQKLDWCDIFF+ T P H RKPRLFQNLPL+ R TLEEYSA
Sbjct: 121 LWQREGDVEGFGQAFVTSEEQKLDWCDIFFIATLPLHFRKPRLFQNLPLSLRETLEEYSA 180

Query: 181 VVKHLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVG 240
            VK +AA I +  EKALGIK+GE++E+FKDGNQSMRMNYYPPCP PEKVIGLTPHSDA G
Sbjct: 181 AVKDVAAEILDGVEKALGIKEGELSELFKDGNQSMRMNYYPPCPEPEKVIGLTPHSDAAG 240

Query: 241 LTILLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSES 300
           LTILLQIN+VEGLKIKK+GNW++V PLPNAF+VNIGDI+EMVTNG+Y+SIEHCATVNS+S
Sbjct: 241 LTILLQINKVEGLKIKKEGNWITVMPLPNAFIVNIGDILEMVTNGEYKSIEHCATVNSKS 300

Query: 301 ERLSIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 360
           ERLSIATF++PS EKEI   PSLITPHSPPLF TLT QEY RG+F+RKLDGKSY DAMRI
Sbjct: 301 ERLSIATFNSPSLEKEIRPTPSLITPHSPPLFTTLTYQEYVRGIFSRKLDGKSYLDAMRI 358

Query: 361 HH 363
           HH
Sbjct: 361 HH 358

[Close]

BLAST of Spg016826 vs. ExPASy TrEMBL
Match: A0A6J1BUE5 (protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111004846 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 6.5e-149
Identity = 276/361 (76.45%), Postives = 302/361 (83.66%), Query Frame = 0


Query: 4   KNNTSSRLGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAASEIPVIDMSSLLS 63
           + N SS  GSSI VP VQELAK+P IEIPPRYVR DL   PI VSAASEIPVID +SLLS
Sbjct: 43  EKNASSTYGSSIPVPSVQELAKQPFIEIPPRYVRSDLLRSPI-VSAASEIPVIDFASLLS 102

Query: 64  DASMESELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQ 123
            ASM+SE  RL SAC++WGFF    QLVNHGV    IEKM+ ETQ+FF L IEE+KKLWQ
Sbjct: 103 GASMDSESARLHSACRDWGFF----QLVNHGVK---IEKMRIETQEFFKLPIEERKKLWQ 162

Query: 124 REGDVEGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVK 183
            EGDVEGFGQ FVVSEEQKLDW DIFFL TAP H RKPRLFQNLPL+FRN LE+YS+ VK
Sbjct: 163 IEGDVEGFGQAFVVSEEQKLDWSDIFFLCTAPEHQRKPRLFQNLPLSFRNALEDYSSEVK 222

Query: 184 HLAAAIFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTI 243
            +AAAI  + E+AL IK+GE+TE+FKDGNQSMRMNYYP CP  EKVIGLTPHSDAVGLTI
Sbjct: 223 DVAAAIVRELERALEIKEGEMTEMFKDGNQSMRMNYYPACPETEKVIGLTPHSDAVGLTI 282

Query: 244 LLQINEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERL 303
           LLQIN+VEGLKIKK+G W+ V PLP+AF+VNIGDIMEM+TNGKYRSIEHCATVNSESERL
Sbjct: 283 LLQINQVEGLKIKKEGKWIPVAPLPDAFIVNIGDIMEMITNGKYRSIEHCATVNSESERL 342

Query: 304 SIATFHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRIHHP 363
           SIATF NPS EK I  APSLITPHSPPLF +LT Q Y  GLF RKLDGKSY DAMRI+HP
Sbjct: 343 SIATFCNPSFEKLIRPAPSLITPHSPPLFTSLTYQHYVSGLFTRKLDGKSYLDAMRINHP 395

Query: 364 P 365
           P
Sbjct: 403 P 395

[Close]

BLAST of Spg016826 vs. ExPASy TrEMBL
Match: A0A1U8AYK0 (protein SRG1-like OS=Nelumbo nucifera OX=4432 GN=LOC104608961 PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 1.3e-125
Identity = 231/354 (65.25%), Postives = 278/354 (78.53%), Query Frame = 0


Query: 11  LGSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAAS--EIPVIDMSSLLSDASME 70
           LGSSILVPCVQELAK+ ++ +PPRYVR    H PI+ +  S   IPVIDM SLLS+ SM+
Sbjct: 14  LGSSILVPCVQELAKQHLVIVPPRYVRSQ--HPPIISNPDSIPTIPVIDMQSLLSEESMD 73

Query: 71  SELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDV 130
           SEL+RL +ACK+WGFF    QLVNHGVS SL+EK K E Q+FF+L +EEKKK WQ + ++
Sbjct: 74  SELSRLHAACKDWGFF----QLVNHGVSSSLVEKTKLEIQEFFNLPLEEKKKFWQLQNEI 133

Query: 131 EGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAA 190
           EGFGQ FVVSE+QKLDW D+FF++T P H RKP LF  LPL  R+TL+ YS  +K LA  
Sbjct: 134 EGFGQAFVVSEDQKLDWGDLFFMSTLPTHRRKPHLFPKLPLPLRDTLDSYSQEMKRLAMN 193

Query: 191 IFEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQIN 250
           I E   KAL ++ GEI E+F+DG QSMRMNYYPPCP PEK IGLTPHSDA GLTILLQ+N
Sbjct: 194 ILELMAKALKMEVGEIRELFEDGMQSMRMNYYPPCPEPEKTIGLTPHSDASGLTILLQVN 253

Query: 251 EVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIATF 310
           E+EGL+++KDG WV V PLPNAF++NIGDI+E+ TNG YRSIEH ATVNS  ERLSIATF
Sbjct: 254 EMEGLQVRKDGMWVPVKPLPNAFIINIGDILEIFTNGTYRSIEHQATVNSVKERLSIATF 313

Query: 311 HNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRIHH 363
            NP+ +KEI  APSLI+  +P LFR + ++EY +GLFARKLDGKSY D MRI +
Sbjct: 314 CNPNFDKEIGPAPSLISSGNPALFRRVASEEYFKGLFARKLDGKSYIDVMRIQN 361

[Close]

BLAST of Spg016826 vs. ExPASy TrEMBL
Match: A0A5B6ZXK1 (Fe2OG dioxygenase domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_017652 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 4.3e-124
Identity = 226/351 (64.39%), Postives = 273/351 (77.78%), Query Frame = 0


Query: 12  GSSILVPCVQELAKKPIIEIPPRYVRPDLGHIPIVVSAAS--EIPVIDMSSLLSDASMES 71
           GSS+LVPCVQELAK+ +  +P RYVRPD    P+ +  A+  ++P+ID+ SL+S    E 
Sbjct: 9   GSSLLVPCVQELAKESLATVPSRYVRPDQDPPPVALRTATSPDVPIIDLESLVSGDRTEI 68

Query: 72  ELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVE 131
           EL +LDSACK WGFF    QL+NHGVS SL+EK+K E Q+FF+L +EEKKKLWQ  GDVE
Sbjct: 69  ELKKLDSACKEWGFF----QLINHGVSTSLMEKVKMEIQEFFNLPMEEKKKLWQEPGDVE 128

Query: 132 GFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAI 191
           GFGQ FVVS+EQKLDW D+FFLTT P HLRKPRLF NLPL FR+TLE Y+  +K L   I
Sbjct: 129 GFGQAFVVSDEQKLDWGDLFFLTTLPSHLRKPRLFPNLPLPFRDTLEIYATELKKLDMKI 188

Query: 192 FEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQINE 251
            +   KAL +K  ++ E+F++G Q+MRMNYYPPCP PE VIGLTPHSD+VGLTILLQ+NE
Sbjct: 189 LDFIAKALKMKAEDMRELFEEGMQAMRMNYYPPCPQPELVIGLTPHSDSVGLTILLQVNE 248

Query: 252 VEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIATFH 311
           +EGL+IKKDG WV V PLPNAFVVNIGDI+E+VTNG YRSIEH A VNS  ERLSIATF+
Sbjct: 249 MEGLQIKKDGMWVPVKPLPNAFVVNIGDILEIVTNGAYRSIEHRAVVNSAKERLSIATFY 308

Query: 312 NPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           +P  + E+  APSLITP +P LF+ +   +Y +GLFARKL GKSY D MRI
Sbjct: 309 SPKLDGEMGPAPSLITPQTPALFKRVAVADYFKGLFARKLHGKSYLDVMRI 355

[Close]

BLAST of Spg016826 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 414.5 bits (1064), Expect = 9.4e-116
Identity = 210/352 (59.66%), Postives = 259/352 (73.58%), Query Frame = 0


Query: 13  SSILVPCVQELAKKPIIE--IPPRYVRPDLGHIPIVV-SAASEIPVIDMSSLLSDASMES 72
           SS++VP VQE+ K+ +I   +PPRYVR D       + S  ++IP+IDMS L S  SM+S
Sbjct: 9   SSVIVPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQIPIIDMSLLSSSTSMDS 68

Query: 73  ELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVE 132
           E+++LD ACK WGFF    QLVNHG+    ++K K++ Q FF+L +EEKKKLWQ+ GD+E
Sbjct: 69  EIDKLDFACKEWGFF----QLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQQPGDIE 128

Query: 133 GFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAI 192
           GFGQ FV SEEQKLDW D+FFLT  P  LRKP LF  LPL FR+TL+ YSA +K +A  +
Sbjct: 129 GFGQAFVFSEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELKSIAKVL 188

Query: 193 FEQFEKALGIKDGEITEIFKDG-NQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQIN 252
           F +   AL IK  E+ ++F D   Q +RMNYYPPCP P+K IGLTPHSDA GLTILLQ+N
Sbjct: 189 FAKLASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLTILLQVN 248

Query: 253 EVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIATF 312
           EVEGL+IKKDG WVSV PLPNA VVN+GDI+E++TNG YRSIEH   VNSE ERLS+A+F
Sbjct: 249 EVEGLQIKKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVASF 308

Query: 313 HNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           HN    KEI    SL+  H   LF+TLTT+EY  GLF+R+LDGK+Y D MRI
Sbjct: 309 HNTGFGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

[Close]

BLAST of Spg016826 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 407.1 bits (1045), Expect = 1.5e-113
Identity = 205/353 (58.07%), Postives = 255/353 (72.24%), Query Frame = 0


Query: 13  SSILVPCVQELAK-KPIIEIPPRYVRPDLGHIPI--VVSAASEIPVIDMSSLLSDASMES 72
           SSILVP VQE+ K K I  +PPRYVR D     +        EIP+IDM  L S  +M+S
Sbjct: 10  SSILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVDDDFDVKIEIPIIDMKRLCSSTTMDS 69

Query: 73  ELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVE 132
           E+ +LD ACK WGFF    QLVNHG+  S ++K+K+E Q FF+L +EEKKK WQR  ++E
Sbjct: 70  EVEKLDFACKEWGFF----QLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIE 129

Query: 133 GFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAI 192
           GFGQ FVVSE+QKLDW D+FF T  P  LRKP LF  LPL FR+TLE YS+ V+ +A  +
Sbjct: 130 GFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKIL 189

Query: 193 FEQFEKALGIKDGEITEIFKDGN--QSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQI 252
             +  +AL IK  E+ ++F D +  QSMRMNYYPPCP P++VIGLTPHSD+VGLT+L+Q+
Sbjct: 190 IAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQV 249

Query: 253 NEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIAT 312
           N+VEGL+IKKDG WV V PLPNAF+VNIGD++E++TNG YRSIEH   VNSE ERLSIAT
Sbjct: 250 NDVEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIAT 309

Query: 313 FHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           FHN    KE+  A SL+       F+ LT +EY  GLF+R LDGK+Y DA+RI
Sbjct: 310 FHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

[Close]

BLAST of Spg016826 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.0e-113
Identity = 207/352 (58.81%), Postives = 256/352 (72.73%), Query Frame = 0


Query: 13  SSILVPCVQELAK-KPIIEIPPRYVRPDLGHIPIVVSAA--SEIPVIDMSSLLSDASMES 72
           SSI+VP VQE+ K K I  +PPRYVR D     I V +   ++IP+IDMS L S  SM+S
Sbjct: 9   SSIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSGLRNQIPIIDMSLLCSSTSMDS 68

Query: 73  ELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVE 132
           E+++LDSACK WGFF    QLVNHG+  S + K+K+E Q FF+L +EEKK LWQ+  ++E
Sbjct: 69  EIDKLDSACKEWGFF----QLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIE 128

Query: 133 GFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAI 192
           GFGQVFVVSEEQKLDW D+FFLT  P  LRKP LF  LPL FR+TL+ YSA VK +A  +
Sbjct: 129 GFGQVFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKIL 188

Query: 193 FEQFEKALGIKDGEITEIFKDG-NQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQIN 252
             +   AL IK  E+ ++F D   Q +R+NYYP CP P+KVIGLTPHSD+ GLTILLQ N
Sbjct: 189 LGKIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQAN 248

Query: 253 EVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIATF 312
           EVEGL+IKK+  WVSV PLPNA VVN+GDI+E++TNG YRSIEH   VNSE ERLS+A F
Sbjct: 249 EVEGLQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAF 308

Query: 313 HNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           HN    KEI    SL+  H    F+++TT+EY  GLF+R+LDGK+Y D MR+
Sbjct: 309 HNIGLGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

[Close]

BLAST of Spg016826 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.0e-109
Identity = 206/353 (58.36%), Postives = 251/353 (71.10%), Query Frame = 0


Query: 13  SSILVPCVQELAK-KPIIEIPPRYVRPDLGHIPIVV---SAASEIPVIDMSSLLSDASME 72
           SSILVP VQE+ K K I  +PPRYVR D     +VV      SEIP+IDM+ L S  +++
Sbjct: 10  SSILVPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLCSSTAVD 69

Query: 73  SELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDV 132
           SE+ +LD ACK +GFF    QLVNHG+  S ++K+K+E Q FF+L +EEKKKLWQ    +
Sbjct: 70  SEVEKLDFACKEYGFF----QLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVM 129

Query: 133 EGFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAA 192
           EGFGQ FVVSE+QKLDW D+FFL   P  LRK  LF  LPL FR+TL+ YS  VK +A  
Sbjct: 130 EGFGQAFVVSEDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKI 189

Query: 193 IFEQFEKALGIKDGEITEIFKDG-NQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQI 252
           +  +  KAL IK  E+ EIF D   QSMRMNYYPPCP P  V GL PHSDAVGLTILLQ+
Sbjct: 190 LLAKMAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQV 249

Query: 253 NEVEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIAT 312
           NEV+GL+IKK+G W  V PL NAF+VN+GD++E++TNG YRSIEH A VN E ERLSIAT
Sbjct: 250 NEVDGLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIAT 309

Query: 313 FHNPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           FHN   +KEI  A SL+       FR+L T++Y  GLF+R+L GK+Y DAMRI
Sbjct: 310 FHNTGMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRI 358

[Close]

BLAST of Spg016826 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.2e-105
Identity = 195/351 (55.56%), Postives = 252/351 (71.79%), Query Frame = 0


Query: 13  SSILVPCVQELAK-KPIIEIPPRYVRPDLGHIPIV--VSAASEIPVIDMSSLLSDASMES 72
           SS++VP V E+ K K    IPPRYVR D     I+   S +SEIPVIDM+ L S ++M+S
Sbjct: 10  SSLIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEILNDSSLSSEIPVIDMTRLCSVSAMDS 69

Query: 73  ELNRLDSACKNWGFFQSTVQLVNHGVSGSLIEKMKAETQKFFDLAIEEKKKLWQREGDVE 132
           EL +LD AC++WGFF    QLVNHG+  S +EK++ E Q+FF+L ++EK+KLWQR G+ E
Sbjct: 70  ELKKLDFACQDWGFF----QLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFE 129

Query: 133 GFGQVFVVSEEQKLDWCDIFFLTTAPPHLRKPRLFQNLPLAFRNTLEEYSAVVKHLAAAI 192
           GFGQV +VSE QKLDW D+F LTT P   RK  LF  LP  FR TLE YS+ VK +A  +
Sbjct: 130 GFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKIL 189

Query: 193 FEQFEKALGIKDGEITEIFKDGNQSMRMNYYPPCPSPEKVIGLTPHSDAVGLTILLQINE 252
           F +    L IK  E+ ++F D  QS+++NYYPPCP P++V+GLT HSDA GLTILLQ+N+
Sbjct: 190 FAKMASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQ 249

Query: 253 VEGLKIKKDGNWVSVTPLPNAFVVNIGDIMEMVTNGKYRSIEHCATVNSESERLSIATFH 312
           VEGL+IKKDG WV V PL +A VVN+G+I+E++TNG+YRSIEH A VNSE ERLS+A FH
Sbjct: 250 VEGLQIKKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFH 309

Query: 313 NPSSEKEISSAPSLITPHSPPLFRTLTTQEYARGLFARKLDGKSYRDAMRI 361
           +P  E  I  A SL+      LF++++TQEY    F +KL+GKS+ D MRI
Sbjct: 310 SPGKETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356

The following BLAST results are available for this feature:

BLAST of Spg016826 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6591778.1	3.0e-164	81.37	Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7024656.1	1.5e-163	81.10	Protein SRG1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022935942.1	4.3e-163	80.82	protein SRG1-like [Cucurbita moschata]	[more]
XP_023534939.1	3.1e-161	80.00	protein SRG1-like [Cucurbita pepo subsp. pepo]	[more]
XP_038897143.1	2.2e-159	78.90	protein SRG1-like [Benincasa hispida]	[more]

BLAST of Spg016826 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39224	2.1e-112	58.07	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]
D4N501	1.1e-105	54.85	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=34...	[more]
D4N500	1.7e-101	54.29	Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1	[more]
D4N502	1.0e-98	51.26	Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1	[more]
A2A1A0	2.8e-72	43.10	S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1	[more]

BLAST of Spg016826 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F704	2.1e-163	80.82	protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111442702 PE=3 SV=1	[more]
A0A1S3CK11	7.2e-156	76.24	probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Cucumis melo OX=3656 GN=...	[more]
A0A6J1BUE5	6.5e-149	76.45	protein SRG1-like OS=Momordica charantia OX=3673 GN=LOC111004846 PE=3 SV=1	[more]
A0A1U8AYK0	1.3e-125	65.25	protein SRG1-like OS=Nelumbo nucifera OX=4432 GN=LOC104608961 PE=3 SV=1	[more]
A0A5B6ZXK1	4.3e-124	64.39	Fe2OG dioxygenase domain-containing protein OS=Davidia involucrata OX=16924 GN=D...	[more]

BLAST of Spg016826 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25310.1	9.4e-116	59.66	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17020.1	1.5e-113	58.07	senescence-related gene 1	[more]
AT4G25300.1	2.0e-113	58.81	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G17010.1	1.0e-109	58.36	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G78550.1	1.2e-105	55.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 53..156 e-value: 9.9E-25 score: 87.6
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 212..311 e-value: 2.5E-27 score: 95.3
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 15..367 e-value: 4.1E-98 score: 330.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 364..378
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 357..378
None	No IPR available	PANTHER	PTHR47991:SF126	2OG-FE(II) OXYGENASE FAMILY OXIDOREDUCTASE	coord: 12..360
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 12..360
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 17..352
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 211..311 score: 14.414647

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016826.1	Spg016826.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding