Spg016816 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016816
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReceptor-like serine/threonine-protein kinase
Locationscaffold9: 37044292 .. 37047677 (-)
RNA-Seq ExpressionSpg016816
SyntenySpg016816
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCAGACAACCAGTCAAGTCGTTGGCAAAACCGAACGAAGCCCAATGCAGGATGGCAAATTTTTCTCTGATATCCTTCGTCGCTGCAACAGTATTTTTATGTTCTTCTTTCAATCTCTCTGTAGCTGCTGATTTCTTAACAGCTTCTCAAAACCTCAGCAATGGCAACACTTTGGTCTCGGAAAAAGGAATTTTCGAGCTGGGTTTCTTTCGTCCAGGAATTTCTAACAACCTTTACTTGGGAATTTGGTACAAAACCATCCCAATTCCCACTGTTGTTTGGGTCGCCAACAGAGAAACCCCACTCAAAGATTCGTCCGGTATTCTGACAATAAATACCACAGCATACAACATTGTTCTCATCCAGAACGATACTGTTGTTTGGTCAGCAAAGTCGCTAAAACCAGTGGAAAGTCCCAGGCTACAGCTCTTGGACACTGGAAATTTAGTCCTGGGAGATGGAAACTCGGAGGAGTTTTTGTGGCAAAGCTTCGATCATCCAACTGATACATTGTTGCCAGGAATGAAGCTGGGATGGGATTTTAAAAATGGCATGAACAGACGATTATCATCTTGGAAAATCTCAGACGACCCATCTCCTGGAACCTTGGCCATGGAAATGGAGAATCATAGCTACCCAGAACTCGCCATGTGGAACGGTACCCAAGAGTTCGTGAGAACTGGACCTTGGAATGGGGTTCGATTCAGCCCCAAATCCGCTGCTGGTCTCCCAATTTTGGTCTACCACTATGTGAATAATAAAGAAGAGCTTTATTTCAGCTACCAGCTCATTAACAACTCTTTGATTGGAAGAATGACGTTGAACCAATCGAGTTTCAGGAGAGAGGTCTTATTGTGGTCAGAAACTGAGAAAAACTGGAAGGTATACGCTACCATACCGAGGGACGACTGTGATAGATACAACTCCTGTGGTGCCTTTGGGAACTGTGACATCGAAACTATGCCTGCTTGTCAATGTCTGAAAGGATTTCGGCCAAGGGTGCCGGAGAAATGGAATGTCATGGATTATACAGAAGGGTGTGTCAGAAATAAGCCTCTGAATTGCTCGGATGAAGTTGGCTTTGCGCAGTTACCAGGGCTGAAATTGCCTGACACTAAATTTTCCTGGGTTAATGAAAGCATGAGTTTAAATGAATGCAGGGAAAAGTGCTTGAGAAATTGTTCTTGTATGGCGTTTGCAAATACAGATATCAGAGGATCAGGTAGTGGTTGTGCCATTTGGCTTGGTGATCTTATTGATATTAAAGTGGTTTTAAAAGGTGGACAAGACTTGTATGTTCGAATACTCGCTTCTGAGTTAGGTATGTGCCTTATTCTCGTTTTTATAGTTTTCCTTCCTAACTCTGTTTCAAGTGTTTTCTTGTTTTCTGTATATTAAATTGAAGATATAAAGAAGGCGAGTTCGGTTGTCATTGGAGTTATAGTTTCTGCTACTGTCCTTATAATTGCCGGCCTGCTTTTGGTTGTATTTTACATTATCAAAAGCAGGAAAATCCTCAAAGGTAAAATTTGCTGCTGAGTTGTTTTCATTTTAAATGACTATGATGTTTTGTGTTGGGCAGTTTTGCAATTCCTGCTACTGATAACTTCTAGGATTGGAAGTGTTAATCAAACCGTAATTTCTAATTCAGAAAGTAAAGCTCAGCATGTGGGTTTTTAGAAGAAATCTAAACTGATCAAGTTATAGTTTGATGATCTTAGCTCCTATATTGTAGATTTGTAGAATGTTTAGATTCAACCCAGGATGAAATAGATCATAGAACCATATAAATCTGATGGAATCTCAACAGGTAAGGAACTTGAGGGGCAAGAGGAAGACTTGGAGCTCCCATTATTTGACTTATCAACAATATCAAATGCCACAGACAATTTTTCAAACTCTAACAAGCTTGGAAAAGGTGGCTTCGGTGCAGTATTCCGGGTAAAATTTAAGTGTACTAAAGAAAACACATTGAGAGTGATGATCAAATGCGATTGACGATCATTGAATTTTCAGGGTAGACTCACAGATGGACAAGAAATTGCTGTAAAGAGGCTTTCCAACTATTCTAGACAAGGAACCAACGAATTCAAGAATGAAGTAATACTTATAGCCAAACTTCAGCATCGGAATCTTGTAAAACTTCTGGGTTGCTGCATCCAAGAGGAAGAAAAAATGTTGATTTATGAATACATGCCTAATAAGAGCTTGGACTCCATCATATTTGGTATGAATATATGGATAACTTTGAGCCTCCGATGTTGGATAATTTTCTTTTTGAACTAACTTTGGCTCATTTGATTTTGGTCAGATCCTAATGGAAGGAAACTCTTAGATTGGTCGAAGCGATTCAATATCATATGTGGAATAGCTAGGGGGATTCTTTATCTTCATGAAGATTCTAGATTGAGGATTATACATAGAGATCTTAAACCAAGTAATGTCTTACTTGATATTGATTTGAATCCAAAAATCTCAGATTTTGGAATGGCTAGAACTTTTGGTGGAGATCAGACGGAAGGAAGTACTAAAAGGGTCGTTGGAACATAGTAAGTACCTTTGGTTTCTATTTAGCTCTATAAGATCACAAATTTTGTTAATAATCCATGAGATTTTTCTTTCTATGTCTTTTATGGTGTCTTACAGTGGATACATGGCGCCAGAGTATGCGATCGATGGACAATTTTCTATTAAGTCAGATGTATTCAGTTTCGGGATTTTATTGTTGGAGATTATAAGCGGAGAGAAAAATAGAGGATTTTATCGTCCAAACCATACTCTCAATCTTATTGGATATGTAAGTTATACCTCGACCCATTTGTCATTAACGATAATCCTCAGCTAAATTGTGTACTTTTTTTTTCCCTATGTTTAGGCATGGAAGTTATGGAAGGAAGGAATACCATTAGAATTGATCGACCCCTCCATTGGGGACTCATATGCTTTATCTGAAGTATTGCGGTGCATCCATGTTAGTCTTTTATGCTTGCAACAACATCCCGAGGATAGACCAATCATGTCAAATGTGATTCTAATGCTAAGTAGTGAGACTGTCTTAGCACAACCAAAACAACCAATATATTACATGGAAAGAGATTCATTTAAAGAGGATTCTCTTTCGGGTAAAAATGAATGTTCTACCTCCAATGAATTAACTGTCACTCTTGTACATGCTAGATAAATTTTGGAATCTTCTCTATCTTCTTTGTATTTCTACAAAAAAAAAAGCGAGTTTTGTATTTTTGTTTTGATATTTTAAAAATTGTTTAGCATTAATTGCAGAATTATCAATTGCTTTCTCAAGAAGGTTGATGAACCAAAAATCGTCGAGAATATATTTAGATAGTTAGTTTATTTAGAAGTTTTTGCTTCATTTATTTTTA

mRNA sequence

ATGACCAGACAACCAGTCAAGTCGTTGGCAAAACCGAACGAAGCCCAATGCAGGATGGCAAATTTTTCTCTGATATCCTTCGTCGCTGCAACAGTATTTTTATGTTCTTCTTTCAATCTCTCTGTAGCTGCTGATTTCTTAACAGCTTCTCAAAACCTCAGCAATGGCAACACTTTGGTCTCGGAAAAAGGAATTTTCGAGCTGGGTTTCTTTCGTCCAGGAATTTCTAACAACCTTTACTTGGGAATTTGGTACAAAACCATCCCAATTCCCACTGTTGTTTGGGTCGCCAACAGAGAAACCCCACTCAAAGATTCGTCCGGTATTCTGACAATAAATACCACAGCATACAACATTGTTCTCATCCAGAACGATACTGTTGTTTGGTCAGCAAAGTCGCTAAAACCAGTGGAAAGTCCCAGGCTACAGCTCTTGGACACTGGAAATTTAGTCCTGGGAGATGGAAACTCGGAGGAGTTTTTGTGGCAAAGCTTCGATCATCCAACTGATACATTGTTGCCAGGAATGAAGCTGGGATGGGATTTTAAAAATGGCATGAACAGACGATTATCATCTTGGAAAATCTCAGACGACCCATCTCCTGGAACCTTGGCCATGGAAATGGAGAATCATAGCTACCCAGAACTCGCCATGTGGAACGGTACCCAAGAGTTCGTGAGAACTGGACCTTGGAATGGGGTTCGATTCAGCCCCAAATCCGCTGCTGGTCTCCCAATTTTGGTCTACCACTATGTGAATAATAAAGAAGAGCTTTATTTCAGCTACCAGCTCATTAACAACTCTTTGATTGGAAGAATGACGTTGAACCAATCGAGTTTCAGGAGAGAGGTCTTATTGTGGTCAGAAACTGAGAAAAACTGGAAGGTATACGCTACCATACCGAGGGACGACTGTGATAGATACAACTCCTGTGGTGCCTTTGGGAACTGTGACATCGAAACTATGCCTGCTTGTCAATGTCTGAAAGGATTTCGGCCAAGGGTGCCGGAGAAATGGAATGTCATGGATTATACAGAAGGGTGTGTCAGAAATAAGCCTCTGAATTGCTCGGATGAAGTTGGCTTTGCGCAGTTACCAGGGCTGAAATTGCCTGACACTAAATTTTCCTGGGTTAATGAAAGCATGAGTTTAAATGAATGCAGGGAAAAGTGCTTGAGAAATTGTTCTTGTATGGCGTTTGCAAATACAGATATCAGAGGATCAGGTAGTGGTTGTGCCATTTGGCTTGGTGATCTTATTGATATTAAAGTGGTTTTAAAAGGTGGACAAGACTTGTATGTTCGAATACTCGCTTCTGAGTTAGATATAAAGAAGGCGAGTTCGGTTGTCATTGGAGTTATAGTTTCTGCTACTGTCCTTATAATTGCCGGCCTGCTTTTGGTTGTATTTTACATTATCAAAAGCAGGAAAATCCTCAAAGGTAAGGAACTTGAGGGGCAAGAGGAAGACTTGGAGCTCCCATTATTTGACTTATCAACAATATCAAATGCCACAGACAATTTTTCAAACTCTAACAAGCTTGGAAAAGGTGGCTTCGGTGCAGTATTCCGGGGTAGACTCACAGATGGACAAGAAATTGCTGTAAAGAGGCTTTCCAACTATTCTAGACAAGGAACCAACGAATTCAAGAATGAAGTAATACTTATAGCCAAACTTCAGCATCGGAATCTTGCATGGAAGTTATGGAAGGAAGGAATACCATTAGAATTGATCGACCCCTCCATTGGGGACTCATATGCTTTATCTGAAGTATTGCGGTGCATCCATGTTAGTCTTTTATGCTTGCAACAACATCCCGAGGATAGACCAATCATGTCAAATGTGATTCTAATGCTAAGTAGTGAGACTGTCTTAGCACAACCAAAACAACCAATATATTACATGGAAAGAGATTCATTTAAAGAGGATTCTCTTTCGGGTAAAAATGAATGTTCTACCTCCAATGAATTAACTGTCACTCTTGTACATGCTAGATAA

Coding sequence (CDS)

ATGACCAGACAACCAGTCAAGTCGTTGGCAAAACCGAACGAAGCCCAATGCAGGATGGCAAATTTTTCTCTGATATCCTTCGTCGCTGCAACAGTATTTTTATGTTCTTCTTTCAATCTCTCTGTAGCTGCTGATTTCTTAACAGCTTCTCAAAACCTCAGCAATGGCAACACTTTGGTCTCGGAAAAAGGAATTTTCGAGCTGGGTTTCTTTCGTCCAGGAATTTCTAACAACCTTTACTTGGGAATTTGGTACAAAACCATCCCAATTCCCACTGTTGTTTGGGTCGCCAACAGAGAAACCCCACTCAAAGATTCGTCCGGTATTCTGACAATAAATACCACAGCATACAACATTGTTCTCATCCAGAACGATACTGTTGTTTGGTCAGCAAAGTCGCTAAAACCAGTGGAAAGTCCCAGGCTACAGCTCTTGGACACTGGAAATTTAGTCCTGGGAGATGGAAACTCGGAGGAGTTTTTGTGGCAAAGCTTCGATCATCCAACTGATACATTGTTGCCAGGAATGAAGCTGGGATGGGATTTTAAAAATGGCATGAACAGACGATTATCATCTTGGAAAATCTCAGACGACCCATCTCCTGGAACCTTGGCCATGGAAATGGAGAATCATAGCTACCCAGAACTCGCCATGTGGAACGGTACCCAAGAGTTCGTGAGAACTGGACCTTGGAATGGGGTTCGATTCAGCCCCAAATCCGCTGCTGGTCTCCCAATTTTGGTCTACCACTATGTGAATAATAAAGAAGAGCTTTATTTCAGCTACCAGCTCATTAACAACTCTTTGATTGGAAGAATGACGTTGAACCAATCGAGTTTCAGGAGAGAGGTCTTATTGTGGTCAGAAACTGAGAAAAACTGGAAGGTATACGCTACCATACCGAGGGACGACTGTGATAGATACAACTCCTGTGGTGCCTTTGGGAACTGTGACATCGAAACTATGCCTGCTTGTCAATGTCTGAAAGGATTTCGGCCAAGGGTGCCGGAGAAATGGAATGTCATGGATTATACAGAAGGGTGTGTCAGAAATAAGCCTCTGAATTGCTCGGATGAAGTTGGCTTTGCGCAGTTACCAGGGCTGAAATTGCCTGACACTAAATTTTCCTGGGTTAATGAAAGCATGAGTTTAAATGAATGCAGGGAAAAGTGCTTGAGAAATTGTTCTTGTATGGCGTTTGCAAATACAGATATCAGAGGATCAGGTAGTGGTTGTGCCATTTGGCTTGGTGATCTTATTGATATTAAAGTGGTTTTAAAAGGTGGACAAGACTTGTATGTTCGAATACTCGCTTCTGAGTTAGATATAAAGAAGGCGAGTTCGGTTGTCATTGGAGTTATAGTTTCTGCTACTGTCCTTATAATTGCCGGCCTGCTTTTGGTTGTATTTTACATTATCAAAAGCAGGAAAATCCTCAAAGGTAAGGAACTTGAGGGGCAAGAGGAAGACTTGGAGCTCCCATTATTTGACTTATCAACAATATCAAATGCCACAGACAATTTTTCAAACTCTAACAAGCTTGGAAAAGGTGGCTTCGGTGCAGTATTCCGGGGTAGACTCACAGATGGACAAGAAATTGCTGTAAAGAGGCTTTCCAACTATTCTAGACAAGGAACCAACGAATTCAAGAATGAAGTAATACTTATAGCCAAACTTCAGCATCGGAATCTTGCATGGAAGTTATGGAAGGAAGGAATACCATTAGAATTGATCGACCCCTCCATTGGGGACTCATATGCTTTATCTGAAGTATTGCGGTGCATCCATGTTAGTCTTTTATGCTTGCAACAACATCCCGAGGATAGACCAATCATGTCAAATGTGATTCTAATGCTAAGTAGTGAGACTGTCTTAGCACAACCAAAACAACCAATATATTACATGGAAAGAGATTCATTTAAAGAGGATTCTCTTTCGGGTAAAAATGAATGTTCTACCTCCAATGAATTAACTGTCACTCTTGTACATGCTAGATAA

Protein sequence

MTRQPVKSLAKPNEAQCRMANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNNLYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVESPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDDPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILASELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHRNLAWKLWKEGIPLELIDPSIGDSYALSEVLRCIHVSLLCLQQHPEDRPIMSNVILMLSSETVLAQPKQPIYYMERDSFKEDSLSGKNECSTSNELTVTLVHAR
Homology
BLAST of Spg016816 vs. NCBI nr
Match: XP_022139494.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139495.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139496.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139497.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139498.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139499.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139500.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139501.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139503.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139504.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139505.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia] >XP_022139506.1 G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Momordica charantia])

HSP 1 Score: 1047.0 bits (2706), Expect = 7.1e-302
Identity = 545/807 (67.53%), Postives = 586/807 (72.61%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MANF L+S V  TVFL SSFN+SVA DFLTASQNLS+GNTLVSEKG FELGFF PG S N
Sbjct: 1   MANFPLMSIV-TTVFLYSSFNISVAVDFLTASQNLSDGNTLVSEKGFFELGFFSPGNSKN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVE 138
            YLGIWYK IPI TVVWVANRE PL +SSGIL INTTA  IVL QN T+VWS KSLK V 
Sbjct: 61  RYLGIWYKIIPISTVVWVANRENPLNNSSGILRINTTANYIVLTQNSTIVWSTKSLKQVG 120

Query: 139 SPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDD 198
           +PRLQLLD GNLVL DGNS EFLWQSFD+PTDTLLPGMKLGWDFKNG+NRRLS+WK SDD
Sbjct: 121 NPRLQLLDNGNLVLKDGNSREFLWQSFDYPTDTLLPGMKLGWDFKNGINRRLSAWKNSDD 180

Query: 199 PSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEEL 258
           PSPGTL MEMENHSYPE AMWNGTQEF+RTGPWNG+R+S KS +GLPILVYHYVNNK EL
Sbjct: 181 PSPGTLNMEMENHSYPEPAMWNGTQEFMRTGPWNGIRYSSKSNSGLPILVYHYVNNKNEL 240

Query: 259 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 318
           YFSYQLINNSLIGRM LNQS FRRE +LWSE EKNWKVYAT+PRD CD YN CGA+G+C+
Sbjct: 241 YFSYQLINNSLIGRMVLNQSMFRREAILWSEAEKNWKVYATMPRDYCDTYNVCGAYGSCN 300

Query: 319 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 378
           IE MP+CQCLKGFRPRV EKWN+MDYTEGCVRNKPLNCSD+VGFA+ PGLKLPDT+ SWV
Sbjct: 301 IEDMPSCQCLKGFRPRVLEKWNLMDYTEGCVRNKPLNCSDKVGFAKFPGLKLPDTRLSWV 360

Query: 379 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 438
           NESMSL+ECREKCLRNCSCMAFANTDIR SGSGCAIWLGDLIDIKVVLKGGQDLYVR+LA
Sbjct: 361 NESMSLSECREKCLRNCSCMAFANTDIRESGSGCAIWLGDLIDIKVVLKGGQDLYVRVLA 420

Query: 439 SELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDL 498
           SELD K+ASSVVIG+IVSA VLIIAGLLLV FYII+SRK L+GKELEGQEEDL+LPLFDL
Sbjct: 421 SELDKKEASSVVIGIIVSAAVLIIAGLLLVGFYIIRSRKSLEGKELEGQEEDLDLPLFDL 480

Query: 499 STISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAK 558
           S ISNATDNFSN NKLG+GGFGAVFRGRLTDGQEIAVKRLS+YS+QG NEFKNEVILIAK
Sbjct: 481 SAISNATDNFSNCNKLGEGGFGAVFRGRLTDGQEIAVKRLSSYSKQGANEFKNEVILIAK 540

Query: 559 LQHRNL------------------------------------------------------ 618
           LQHRNL                                                      
Sbjct: 541 LQHRNLVKLLGCCIHEKERMLIYEYMPNKSLDSFIFDSAGRNLLDWSKRFNIICGIARGI 600

Query: 619 ------------------------------------------------------------ 666
                                                                       
Sbjct: 601 LYLHQDSRLRIIHRDLKPSNVLLDIDMNPKISDFGMARTFGGDQTEGNTRRVVGTYGYMA 660

BLAST of Spg016816 vs. NCBI nr
Match: XP_038897080.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 [Benincasa hispida])

HSP 1 Score: 1030.0 bits (2662), Expect = 8.9e-297
Identity = 544/823 (66.10%), Postives = 586/823 (71.20%), Query Frame = 0

Query: 6   VKSLAKPNEAQCRMANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGI 65
           V S  KPN+ QCRMANF LISFV  T FL  SFN+SVA DFLTASQNLS+GNTLVSEKGI
Sbjct: 15  VVSKTKPNQTQCRMANFHLISFV-MTTFLYFSFNISVAVDFLTASQNLSDGNTLVSEKGI 74

Query: 66  FELGFFRPGISNNLYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQND 125
           FELGFFRPGIS N YLGIWYK IPIPTVVWVANRETPLKD S IL INTTA ++VLIQN+
Sbjct: 75  FELGFFRPGISKNRYLGIWYKIIPIPTVVWVANRETPLKDFSSILRINTTANHVVLIQNE 134

Query: 126 TVVWSAKSLKPVESPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNG 185
           TV+WSAKSLKP+E+PRLQLLDTGNL L DGNSEE LWQSFD+PTDTLLPGMKLGWD++NG
Sbjct: 135 TVIWSAKSLKPMENPRLQLLDTGNLALKDGNSEEILWQSFDYPTDTLLPGMKLGWDYENG 194

Query: 186 MNRRLSSWKISDDPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLP 245
           +NRRLS+WK  DDPSPGTL +EMENHS+PELAMWNGTQE VRTGPWNG+RFS KS +GLP
Sbjct: 195 INRRLSAWKNWDDPSPGTLILEMENHSFPELAMWNGTQEIVRTGPWNGLRFSSKSISGLP 254

Query: 246 ILVYHYVNNKEELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDC 305
           ILVYHYVNN  ELYF+YQLINNS IGRM LNQS FRRE LLWSE EK WK+YATIPRD C
Sbjct: 255 ILVYHYVNNNNELYFNYQLINNSSIGRMVLNQSRFRREALLWSEAEKKWKIYATIPRDYC 314

Query: 306 DRYNSCGAFGNCDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQL 365
           D Y+ CGA+GNCDIE MPACQCLKGF+PRVPE W  MDY EGCVRNK LNCSDEVGFA+L
Sbjct: 315 DTYSICGAYGNCDIENMPACQCLKGFQPRVPENWYRMDYVEGCVRNKSLNCSDEVGFAKL 374

Query: 366 PGLKLPDTKFSWVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVV 425
           PGLKLPDT +SWVNESMSL+ECREKCLRNCSCMAFANT+I  SGSGCAIW  DLIDIKVV
Sbjct: 375 PGLKLPDTTYSWVNESMSLSECREKCLRNCSCMAFANTNISASGSGCAIWFNDLIDIKVV 434

Query: 426 LKGGQDLYVRILASELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELE 485
           +KGGQDLYVRILASELD K  SSVV+G IVSAT+LIIA L+LV FYIIKS KIL+ ++LE
Sbjct: 435 IKGGQDLYVRILASELDKKNVSSVVVGFIVSATLLIIAALVLVGFYIIKSSKILQVEKLE 494

Query: 486 GQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQG 545
            QEEDLELPLFDLSTI NATDNFSNSNKLG+GGFG+VFRG L DGQEIAVKRLSNYSRQG
Sbjct: 495 VQEEDLELPLFDLSTILNATDNFSNSNKLGEGGFGSVFRGILKDGQEIAVKRLSNYSRQG 554

Query: 546 TNEFKNEVILIAKLQHRNL----------------------------------------- 605
           TNEFKNE+ LIAKLQHRNL                                         
Sbjct: 555 TNEFKNELKLIAKLQHRNLVKLLGCCIQEEEKMLIYEYMPNKSLDSLIFDPNGRKLLDWP 614

Query: 606 ------------------------------------------------------------ 665
                                                                       
Sbjct: 615 KRFNIICGIVRGILYLHEDSRLRIIHRDLKPSNVLLDIDMNPKISDFGMARTFGGDQTEG 674

BLAST of Spg016816 vs. NCBI nr
Match: XP_031738392.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X2 [Cucumis sativus] >KGN59993.2 hypothetical protein Csa_002302 [Cucumis sativus])

HSP 1 Score: 994.6 bits (2570), Expect = 4.2e-286
Identity = 526/811 (64.86%), Postives = 575/811 (70.90%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MA+F LISFV A V L SSFN+ VA DFLT+SQNL++GNTLVSEKGIFELGFFRPGISNN
Sbjct: 1   MASFLLISFVTAMV-LFSSFNVYVAVDFLTSSQNLTHGNTLVSEKGIFELGFFRPGISNN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVE 138
            YLGIWYKTIPIPTVVWVANRETPL D S IL INTTA ++VLIQN TV+WSAKSLKP+E
Sbjct: 61  RYLGIWYKTIPIPTVVWVANRETPLVDFSSILIINTTANHVVLIQNKTVIWSAKSLKPME 120

Query: 139 SPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDD 198
           +PRLQLLDTGNL L DG SEE LWQSFD+PTDTLLPGMKLGWD++NG+NRRLS+WK  DD
Sbjct: 121 NPRLQLLDTGNLALKDGKSEEILWQSFDYPTDTLLPGMKLGWDYENGINRRLSAWKNWDD 180

Query: 199 PSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEEL 258
           PSPGTL +EMENHSYPELAMWNGTQE VRTGPWNG+RFS KS +GLPILVYHYVNNK EL
Sbjct: 181 PSPGTLILEMENHSYPELAMWNGTQEIVRTGPWNGMRFSSKSISGLPILVYHYVNNKNEL 240

Query: 259 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 318
           YFS+QLINNSLIGRM LNQS  RRE LLWSE EKNW +YATIPRD CD YN CGA+GNCD
Sbjct: 241 YFSFQLINNSLIGRMVLNQSRSRREALLWSEAEKNWMIYATIPRDYCDTYNVCGAYGNCD 300

Query: 319 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 378
           IE MPACQCLKGF+PRV E WN MDYTEGCVR K LNC DEVGFA+LPG+KLPDT +SWV
Sbjct: 301 IENMPACQCLKGFQPRVLENWNQMDYTEGCVRTKHLNCWDEVGFAKLPGMKLPDTTYSWV 360

Query: 379 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 438
           NESMSL+ECREKCLRNCSCMAFANTDIRG GSGCAIWL DL+DIKVV+KGGQDLYVR+LA
Sbjct: 361 NESMSLSECREKCLRNCSCMAFANTDIRGLGSGCAIWLNDLLDIKVVIKGGQDLYVRMLA 420

Query: 439 SELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDL 498
           SELD  KA+ V+IGVIVSAT+LIIA L+LV FYIIK RKIL+ ++L+ QEEDLELPLFDL
Sbjct: 421 SELDTTKANLVIIGVIVSATLLIIAALVLVGFYIIKRRKILEVEKLDVQEEDLELPLFDL 480

Query: 499 STISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAK 558
           ST+SNATDNFSNS KLG+GGFG VF G+L DG+EIAVKRLSNYSRQGTNEFKNEV LIAK
Sbjct: 481 STVSNATDNFSNSKKLGEGGFGVVFWGKLKDGREIAVKRLSNYSRQGTNEFKNEVKLIAK 540

Query: 559 LQHRNL------------------------------------------------------ 618
           LQHRNL                                                      
Sbjct: 541 LQHRNLVKLLGCCIQEREKMLIYEYMPNKSLSSLIFDPNGRKLLDWSKRFNIICGIVRGI 600

Query: 619 ------------------------------------------------------------ 666
                                                                       
Sbjct: 601 LYLHEDSRLRIIHRDLKPSNILLDIDMNPKISDFGMARTFGGDQTEGNTRRVVGTYGYMA 660

BLAST of Spg016816 vs. NCBI nr
Match: XP_022139507.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X2 [Momordica charantia])

HSP 1 Score: 982.2 bits (2538), Expect = 2.1e-282
Identity = 514/807 (63.69%), Postives = 551/807 (68.28%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MANF L+S V  TVFL SSFN+SVA DFLTASQNLS+GNTLVSEKG FELGFF PG S N
Sbjct: 1   MANFPLMSIV-TTVFLYSSFNISVAVDFLTASQNLSDGNTLVSEKGFFELGFFSPGNSKN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVE 138
            YLGIWYK IPI TVVWVANRE PL +SSGIL INTTA  IVL QN T+VWS KSLK V 
Sbjct: 61  RYLGIWYKIIPISTVVWVANRENPLNNSSGILRINTTANYIVLTQNSTIVWSTKSLKQVG 120

Query: 139 SPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDD 198
           +PRLQLLD GNLVL DGNS EFLWQSFD+PTDTLLPGMKLGWDFKNG+NRRLS+WK SDD
Sbjct: 121 NPRLQLLDNGNLVLKDGNSREFLWQSFDYPTDTLLPGMKLGWDFKNGINRRLSAWKNSDD 180

Query: 199 PSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEEL 258
           PSPGTL MEMENHSYPE AMWNGTQEF+RTGPWNG+R+S KS +GLPILVYHYVNNK EL
Sbjct: 181 PSPGTLNMEMENHSYPEPAMWNGTQEFMRTGPWNGIRYSSKSNSGLPILVYHYVNNKNEL 240

Query: 259 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 318
           YFSYQLINNSLIGRM LNQS FRRE +LWSE EKNWKVYAT+PRD CD YN CGA+G+C+
Sbjct: 241 YFSYQLINNSLIGRMVLNQSMFRREAILWSEAEKNWKVYATMPRDYCDTYNVCGAYGSCN 300

Query: 319 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 378
           IE MP+CQCLKGFRPRV EKWN+MDYTEGCVRNKPLNCSD+VGFA+ PGLKLPDT+ SWV
Sbjct: 301 IEDMPSCQCLKGFRPRVLEKWNLMDYTEGCVRNKPLNCSDKVGFAKFPGLKLPDTRLSWV 360

Query: 379 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 438
           NESMSL+ECREKCLRNCSCMAFANTDIR SGSGCAIWLGDLIDIKVVLKGGQDLYVR+LA
Sbjct: 361 NESMSLSECREKCLRNCSCMAFANTDIRESGSGCAIWLGDLIDIKVVLKGGQDLYVRVLA 420

Query: 439 SELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDL 498
           SEL                                       GKELEGQEEDL+LPLFDL
Sbjct: 421 SEL---------------------------------------GKELEGQEEDLDLPLFDL 480

Query: 499 STISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAK 558
           S ISNATDNFSN NKLG+GGFGAVFRGRLTDGQEIAVKRLS+YS+QG NEFKNEVILIAK
Sbjct: 481 SAISNATDNFSNCNKLGEGGFGAVFRGRLTDGQEIAVKRLSSYSKQGANEFKNEVILIAK 540

Query: 559 LQHRNL------------------------------------------------------ 618
           LQHRNL                                                      
Sbjct: 541 LQHRNLVKLLGCCIHEKERMLIYEYMPNKSLDSFIFDSAGRNLLDWSKRFNIICGIARGI 600

Query: 619 ------------------------------------------------------------ 666
                                                                       
Sbjct: 601 LYLHQDSRLRIIHRDLKPSNVLLDIDMNPKISDFGMARTFGGDQTEGNTRRVVGTYGYMA 660

BLAST of Spg016816 vs. NCBI nr
Match: XP_031738391.1 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X1 [Cucumis sativus])

HSP 1 Score: 979.2 bits (2530), Expect = 1.8e-281
Identity = 526/844 (62.32%), Postives = 576/844 (68.25%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MA+F LISFV A V L SSFN+ VA DFLT+SQNL++GNTLVSEKGIFELGFFRPGISNN
Sbjct: 1   MASFLLISFVTAMV-LFSSFNVYVAVDFLTSSQNLTHGNTLVSEKGIFELGFFRPGISNN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVE 138
            YLGIWYKTIPIPTVVWVANRETPL D S IL INTTA ++VLIQN TV+WSAKSLKP+E
Sbjct: 61  RYLGIWYKTIPIPTVVWVANRETPLVDFSSILIINTTANHVVLIQNKTVIWSAKSLKPME 120

Query: 139 SPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDD 198
           +PRLQLLDTGNL L DG SEE LWQSFD+PTDTLLPGMKLGWD++NG+NRRLS+WK  DD
Sbjct: 121 NPRLQLLDTGNLALKDGKSEEILWQSFDYPTDTLLPGMKLGWDYENGINRRLSAWKNWDD 180

Query: 199 PSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEEL 258
           PSPGTL +EMENHSYPELAMWNGTQE VRTGPWNG+RFS KS +GLPILVYHYVNNK EL
Sbjct: 181 PSPGTLILEMENHSYPELAMWNGTQEIVRTGPWNGMRFSSKSISGLPILVYHYVNNKNEL 240

Query: 259 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 318
           YFS+QLINNSLIGRM LNQS  RRE LLWSE EKNW +YATIPRD CD YN CGA+GNCD
Sbjct: 241 YFSFQLINNSLIGRMVLNQSRSRREALLWSEAEKNWMIYATIPRDYCDTYNVCGAYGNCD 300

Query: 319 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 378
           IE MPACQCLKGF+PRV E WN MDYTEGCVR K LNC DEVGFA+LPG+KLPDT +SWV
Sbjct: 301 IENMPACQCLKGFQPRVLENWNQMDYTEGCVRTKHLNCWDEVGFAKLPGMKLPDTTYSWV 360

Query: 379 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 438
           NESMSL+ECREKCLRNCSCMAFANTDIRG GSGCAIWL DL+DIKVV+KGGQDLYVR+LA
Sbjct: 361 NESMSLSECREKCLRNCSCMAFANTDIRGLGSGCAIWLNDLLDIKVVIKGGQDLYVRMLA 420

Query: 439 SELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDL 498
           SELD  KA+ V+IGVIVSAT+LIIA L+LV FYIIK RKIL+ ++L+ QEEDLELPLFDL
Sbjct: 421 SELDTTKANLVIIGVIVSATLLIIAALVLVGFYIIKRRKILEVEKLDVQEEDLELPLFDL 480

Query: 499 STISNATDNFSNSNKLGKGGFGAV---------------------------------FRG 558
           ST+SNATDNFSNS KLG+GGFG V                                 F+G
Sbjct: 481 STVSNATDNFSNSKKLGEGGFGVVFWVKLKCTKIIYFKTDDHNSMSLIDYKNFLSMNFQG 540

Query: 559 RLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHRNL--------------------- 618
           +L DG+EIAVKRLSNYSRQGTNEFKNEV LIAKLQHRNL                     
Sbjct: 541 KLKDGREIAVKRLSNYSRQGTNEFKNEVKLIAKLQHRNLVKLLGCCIQEREKMLIYEYMP 600

Query: 619 ------------------------------------------------------------ 666
                                                                       
Sbjct: 601 NKSLSSLIFDPNGRKLLDWSKRFNIICGIVRGILYLHEDSRLRIIHRDLKPSNILLDIDM 660

BLAST of Spg016816 vs. ExPASy Swiss-Prot
Match: O81905 (Receptor-like serine/threonine-protein kinase SD1-8 OS=Arabidopsis thaliana OX=3702 GN=SD18 PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 7.9e-147
Identity = 310/832 (37.26%), Postives = 429/832 (51.56%), Query Frame = 0

Query: 41  SVAADFLTASQNL--SNGNTLVSEKGIFELGFFRPGISNNLYLGIWYKTIPIPTVVWVAN 100
           S++A+ L+AS++L  S+ NT+VS   +FELGFF+PG+ +  YLGIWYK I   T VWVAN
Sbjct: 26  SISANTLSASESLTISSNNTIVSPGNVFELGFFKPGLDSRWYLGIWYKAISKRTYVWVAN 85

Query: 101 RETPLKDSSGILTINTTAYNIVLIQNDTVVWSAK-SLKPVESPRL-QLLDTGNLVLGDGN 160
           R+TPL  S G L I+ +   +VL Q+DT VWS   +   V SP + +LLD GN VL D  
Sbjct: 86  RDTPLSSSIGTLKISDSNL-VVLDQSDTPVWSTNLTGGDVRSPLVAELLDNGNFVLRDSK 145

Query: 161 S---EEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDDPSPGTLAMEMENHSY 220
           +   +  LWQSFD PTDTLLP MKLGWD K G NR + SWK  DDPS G  + ++E   +
Sbjct: 146 NSAPDGVLWQSFDFPTDTLLPEMKLGWDAKTGFNRFIRSWKSPDDPSSGDFSFKLETEGF 205

Query: 221 PELAMWNGTQEFVRTGPWNGVRFSPKSAAGLP------ILVYHYVNNKEELYFSYQLINN 280
           PE+ +WN      R+GPWNG+RFS     G+P       +V+++  +KEE+ +S+++  +
Sbjct: 206 PEIFLWNRESRMYRSGPWNGIRFS-----GVPEMQPFEYMVFNFTTSKEEVTYSFRITKS 265

Query: 281 SLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCDIETMPACQC 340
            +  R++++ S   +    W ET +NW  +   P+D CD Y  CG +G CD  T P C C
Sbjct: 266 DVYSRLSISSSGLLQR-FTWIETAQNWNQFWYAPKDQCDEYKECGVYGYCDSNTSPVCNC 325

Query: 341 LKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWVNESMSLNEC 400
           +KGF+PR P+ W + D ++GCVR   L+C    GF +L  +KLPDT  + V+  + + EC
Sbjct: 326 IKGFKPRNPQVWGLRDGSDGCVRKTLLSCGGGDGFVRLKKMKLPDTTTASVDRGIGVKEC 385

Query: 401 REKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILASELDIKK-A 460
            +KCLR+C+C AFANTDIRGSGSGC  W G+L DI+   KGGQDLYVR+ A++L+ K+  
Sbjct: 386 EQKCLRDCNCTAFANTDIRGSGSGCVTWTGELFDIRNYAKGGQDLYVRLAATDLEDKRNR 445

Query: 461 SSVVIGVIVSATVLIIAGLLL-----------------VVFYIIKSRKILKGKEL----- 520
           S+ +IG  +  +VL++   ++                 +V + ++SR +L  + +     
Sbjct: 446 SAKIIGSSIGVSVLLLLSFIIFFLWKRKQKRSILIETPIVDHQLRSRDLLMNEVVISSRR 505

Query: 521 ----EGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSN 580
               E   +DLELPL +   ++ AT+NFSN+NKLG+GGFG V++G+L DGQE+AVKRLS 
Sbjct: 506 HISRENNTDDLELPLMEFEEVAMATNNFSNANKLGQGGFGIVYKGKLLDGQEMAVKRLSK 565

Query: 581 YSRQGTNEFKNEVILIAKLQHRNL------------------------------------ 640
            S QGT+EFKNEV LIA+LQH NL                                    
Sbjct: 566 TSVQGTDEFKNEVKLIARLQHINLVRLLACCVDAGEKMLIYEYLENLSLDSHLFDKSRNS 625

Query: 641 ------------------------------------------------------------ 666
                                                                       
Sbjct: 626 KLNWQMRFDIINGIARGLLYLHQDSRFRIIHRDLKASNILLDKYMTPKISDFGMARIFGR 685

BLAST of Spg016816 vs. ExPASy Swiss-Prot
Match: O81832 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 OS=Arabidopsis thaliana OX=3702 GN=At4g27290 PE=3 SV=4)

HSP 1 Score: 491.1 bits (1263), Expect = 2.0e-137
Identity = 288/804 (35.82%), Postives = 407/804 (50.62%), Query Frame = 0

Query: 32  VFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNNLYLGIWYKTIPIP 91
           + L S+  L+ A D L A+Q L +G+T+VS+ G FE+GFF PG S N YLGIWYK I + 
Sbjct: 12  ISLFSTILLAQATDILIANQTLKDGDTIVSQGGSFEVGFFSPGGSRNRYLGIWYKKISLQ 71

Query: 92  TVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKS-----LKPVESPRLQLLD 151
           TVVWVANR++PL D SG L ++      +    + ++WS+ S        + +P +Q+LD
Sbjct: 72  TVVWVANRDSPLYDLSGTLKVSENGSLCLFNDRNHIIWSSSSSPSSQKASLRNPIVQILD 131

Query: 152 TGNLVL-GDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDDPSPGTLA 211
           TGNLV+   G+ ++++WQS D+P D  LPGMK G +F  G+NR L+SW+  DDPS G   
Sbjct: 132 TGNLVVRNSGDDQDYIWQSLDYPGDMFLPGMKYGLNFVTGLNRFLTSWRAIDDPSTGNYT 191

Query: 212 MEMENHSYPELAMWNGTQEFVRTGPWNGVRFS-PKSAAGLPILVYHYVNNKEELYFSYQL 271
            +M+ +  P+  +   +    RTGPWNG+RF+   +    PI  Y YV  +EE+Y++Y+L
Sbjct: 192 NKMDPNGVPQFFLKKNSVVVFRTGPWNGLRFTGMPNLKPNPIYRYEYVFTEEEVYYTYKL 251

Query: 272 INNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCDIETMPA 331
            N S++ RM LN +   +    W +  ++W  Y +   D CD+Y  CG++G+C+I   PA
Sbjct: 252 ENPSVLTRMQLNPNGALQR-YTWVDNLQSWNFYLSAMMDSCDQYTLCGSYGSCNINESPA 311

Query: 332 CQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNC-SDEVGFAQLPGLKLPDTKFSWVNESMS 391
           C+CLKGF  + P+ W   D++EGCVR   L+C   E GF ++  LKLPDT+ SW +++M 
Sbjct: 312 CRCLKGFVAKTPQAWVAGDWSEGCVRRVKLDCGKGEDGFLKISKLKLPDTRTSWYDKNMD 371

Query: 392 LNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILASELD- 451
           LNEC++ CLRNC+C A++  DIR  G GC +W GDLIDI+   + GQDLYVR+ +SE++ 
Sbjct: 372 LNECKKVCLRNCTCSAYSPFDIRDGGKGCILWFGDLIDIREYNENGQDLYVRLASSEIET 431

Query: 452 IKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDLSTIS 511
           +++ SS V                        SRK         +EEDLELP  DL T+S
Sbjct: 432 LQRESSRV-----------------------SSRK--------QEEEDLELPFLDLDTVS 491

Query: 512 NATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHR 571
            AT  FS  NKLG+GGFG V++G L  GQE+AVKRLS  SRQG  EFKNE+ LIAKLQHR
Sbjct: 492 EATSGFSAGNKLGQGGFGPVYKGTLACGQEVAVKRLSRTSRQGVEEFKNEIKLIAKLQHR 551

Query: 572 NL---------------------------------------------------------- 631
           NL                                                          
Sbjct: 552 NLVKILGYCVDEEERMLIYEYQPNKSLDSFIFDKERRRELDWPKRVEIIKGIARGMLYLH 611

Query: 632 ------------------------------------------------------------ 666
                                                                       
Sbjct: 612 EDSRLRIIHRDLKASNVLLDSDMNAKISDFGLARTLGGDETEANTTRVVGTYGYMSPEYQ 671

BLAST of Spg016816 vs. ExPASy Swiss-Prot
Match: Q39086 (Receptor-like serine/threonine-protein kinase SD1-7 OS=Arabidopsis thaliana OX=3702 GN=SD17 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 7.7e-134
Identity = 294/845 (34.79%), Postives = 417/845 (49.35%), Query Frame = 0

Query: 21  NFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNNLY 80
           N+    F+   + L  +F++S      T S  +S+  T++S   IFELGFF P  S+  Y
Sbjct: 6   NYHHSFFIFLILILFLAFSVSPNTLSATESLTISSNKTIISPSQIFELGFFNPASSSRWY 65

Query: 81  LGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLI-QNDTVVWSAK-SLKPVE 140
           LGIWYK IPI T VWVANR+ PL  S+G  T+  +  N+V+  Q+D  VWS   +   V 
Sbjct: 66  LGIWYKIIPIRTYVWVANRDNPLSSSNG--TLKISGNNLVIFDQSDRPVWSTNITGGDVR 125

Query: 141 SP-RLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISD 200
           SP   +LLD GN +L D N+   LWQSFD PTDTLL  MKLGWD K G NR L SWK +D
Sbjct: 126 SPVAAELLDNGNFLLRDSNN-RLLWQSFDFPTDTLLAEMKLGWDQKTGFNRILRSWKTTD 185

Query: 201 DPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSP-KSAAGLPILVYHYVNNKE 260
           DPS G  + ++E   +PE  + +      R+GPWNG+RFS       +  +VY++  +KE
Sbjct: 186 DPSSGEFSTKLETSEFPEFYICSKESILYRSGPWNGMRFSSVPGTIQVDYMVYNFTASKE 245

Query: 261 ELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGN 320
           E+ +SY++   +L  R+ LN +   +  L W ET ++WK     P+D CD Y  CG FG 
Sbjct: 246 EVTYSYRINKTNLYSRLYLNSAGLLQR-LTWFETTQSWKQLWYSPKDLCDNYKVCGNFGY 305

Query: 321 CDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFS 380
           CD  ++P C C+KGF+P   + W++ D + GC+R   L+C    GF +L  +KLPDT  +
Sbjct: 306 CDSNSLPNCYCIKGFKPVNEQAWDLRDGSAGCMRKTRLSCDGRDGFTRLKRMKLPDTTAT 365

Query: 381 WVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRI 440
            V+  + L  C+E+CL +C+C AFAN DIR  GSGC IW  +++D++   KGGQDLYVR+
Sbjct: 366 IVDREIGLKVCKERCLEDCNCTAFANADIRNGGSGCVIWTREILDMRNYAKGGQDLYVRL 425

Query: 441 LASELDIKK-ASSVVIGVIVSATVLIIAGLLLVVFY------------------------ 500
            A+EL+ K+  +  +IG  +  ++L++   ++  F+                        
Sbjct: 426 AAAELEDKRIKNEKIIGSSIGVSILLLLSFVIFHFWKRKQKRSITIQTPNVDQVRSQDSL 485

Query: 501 ---IIKSRKILKGKELEGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLT 560
              ++ SR+    K  E + E LELPL +L  ++ AT+NFSN NKLG+GGFG V++GRL 
Sbjct: 486 INDVVVSRRGYTSK--EKKSEYLELPLLELEALATATNNFSNDNKLGQGGFGIVYKGRLL 545

Query: 561 DGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHRNL------------------------ 620
           DG+EIAVKRLS  S QGT+EF NEV LIAKLQH NL                        
Sbjct: 546 DGKEIAVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYLENLS 605

Query: 621 ------------------------------------------------------------ 666
                                                                       
Sbjct: 606 LDSHLFDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKNMTPK 665

BLAST of Spg016816 vs. ExPASy Swiss-Prot
Match: Q9S972 (Receptor-like serine/threonine-protein kinase SD1-6 OS=Arabidopsis thaliana OX=3702 GN=SD16 PE=1 SV=2)

HSP 1 Score: 449.5 bits (1155), Expect = 6.5e-125
Identity = 288/849 (33.92%), Postives = 412/849 (48.53%), Query Frame = 0

Query: 21  NFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNNLY 80
           N+    F+   + L  +F++  +    T S  +S+  T++S   IFELGFF P  S+  Y
Sbjct: 6   NYHHSYFILFIIILFLAFSVYASNFSATESLTISSNKTIISPSQIFELGFFNPDSSSRWY 65

Query: 81  LGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLI-QNDTVVWSAK-SLKPVE 140
           LGIWYK IPI T VWVANR+ PL  S+G L I+    N+V+  Q+D  VWS   +   V 
Sbjct: 66  LGIWYKIIPIRTYVWVANRDNPLSSSNGTLKISDN--NLVIFDQSDRPVWSTNITGGDVR 125

Query: 141 SP-RLQLLDTGNLVLGDGNSEE---FLWQSFDHPTDTLLPGMKLGWDFKN-GMNRRLSSW 200
           SP   +LLD GN VL D  + +   FLWQSFD PTDTLL  MK+GWD K+ G NR L SW
Sbjct: 126 SPVAAELLDYGNFVLRDSKNNKPSGFLWQSFDFPTDTLLSDMKMGWDNKSGGFNRILRSW 185

Query: 201 KISDDPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVY---H 260
           K +DDPS G  + ++    +PE  ++N      R+GPW G RFS  S  G+  + Y    
Sbjct: 186 KTTDDPSSGDFSTKLRTSGFPEFYIYNKESITYRSGPWLGNRFS--SVPGMKPVDYIDNS 245

Query: 261 YVNNKEELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNS 320
           +  N +++ +SY++   ++   ++L+ +   +  L W E  ++WK     P+D CD Y  
Sbjct: 246 FTENNQQVVYSYRVNKTNIYSILSLSSTGLLQR-LTWMEAAQSWKQLWYSPKDLCDNYKE 305

Query: 321 CGAFGNCDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKL 380
           CG +G CD  T P C C+KGF P + E+  + D + GCVR   L+C    GF +L  ++L
Sbjct: 306 CGNYGYCDANTSPICNCIKGFEP-MNEQAALRDDSVGCVRKTKLSCDGRDGFVRLKKMRL 365

Query: 381 PDTKFSWVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQ 440
           PDT  + V++ + L EC E+CL+ C+C AFANTDIR  GSGC IW G L DI+   KGGQ
Sbjct: 366 PDTTETSVDKGIGLKECEERCLKGCNCTAFANTDIRNGGSGCVIWSGGLFDIRNYAKGGQ 425

Query: 441 DLYVRILASELDIKK-ASSVVIGVIVSATVLIIAGLLLVVFY----------------II 500
           DLYVR+ A +L+ K+  S  +IG  +  ++L++   ++  F+                ++
Sbjct: 426 DLYVRVAAGDLEDKRIKSKKIIGSSIGVSILLLLSFIIFHFWKRKQKRSITIQTPIVDLV 485

Query: 501 KSRKILKGK---------ELEGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFR 560
           +S+  L  +           E + + LELPL +   ++ AT+NFS  NKLG+GGFG V++
Sbjct: 486 RSQDSLMNELVKASRSYTSKENKTDYLELPLMEWKALAMATNNFSTDNKLGQGGFGIVYK 545

Query: 561 GRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHRNL-------------------- 620
           G L DG+EIAVKRLS  S QGT+EF NEV LIAKLQH NL                    
Sbjct: 546 GMLLDGKEIAVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYL 605

Query: 621 ------------------------------------------------------------ 666
                                                                       
Sbjct: 606 ENLSLDSHLFDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKN 665

BLAST of Spg016816 vs. ExPASy Swiss-Prot
Match: P0DH86 (G-type lectin S-receptor-like serine/threonine-protein kinase SRK OS=Arabidopsis thaliana OX=3702 GN=SRK PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 7.2e-124
Identity = 243/571 (42.56%), Postives = 349/571 (61.12%), Query Frame = 0

Query: 27  FVAATVFLCSSFNLSVAADFLTASQNL--SNGNTLVSEKGIFELGFFRPGISNNLYLGIW 86
           FV    FL    +LS++ + L+A+++L  S+  T+VS  G+FELGFFR  + ++ YLGIW
Sbjct: 15  FVFLFFFLILFPDLSISVNTLSATESLTISSNKTIVSPGGVFELGFFRI-LGDSWYLGIW 74

Query: 87  YKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQN-DTVVWSAKSLKPVESPRL- 146
           YK I   T VWVANR+TPL +  GIL I+    N+V++ N DT VWS      V S  + 
Sbjct: 75  YKKISQRTYVWVANRDTPLSNPIGILKISNA--NLVILDNSDTHVWSTNLTGAVRSSVVA 134

Query: 147 QLLDTGNLVLGD---GNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDDP 206
           +LLD GN VL       S+EFLWQSFD PTDTLLP MKLG D K G+NR ++SWK S DP
Sbjct: 135 ELLDNGNFVLRGSKINESDEFLWQSFDFPTDTLLPQMKLGRDHKRGLNRFVTSWKSSFDP 194

Query: 207 SPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSP-KSAAGLPILVYHYVNNKEEL 266
           S G+   ++E    PE   +    E  R+GPW+G+RFS          ++Y++  N+EE+
Sbjct: 195 SSGSFMFKLETLGLPEFFGFTSFLEVYRSGPWDGLRFSGILEMQQWDDIIYNFTENREEV 254

Query: 267 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 326
            +++++ +++   R+T+N    R E  +W  T++ W ++  +P+D CD Y  CG +  CD
Sbjct: 255 AYTFRVTDHNSYSRLTINTVG-RLEGFMWEPTQQEWNMFWFMPKDTCDLYGICGPYAYCD 314

Query: 327 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 386
           + T P C C+KGF+P  P+ W   D T  C R   L C ++  F +L  +K+P T  + V
Sbjct: 315 MSTSPTCNCIKGFQPLSPQDWASGDVTGRCRRKTQLTCGED-RFFRLMNMKIPATTAAIV 374

Query: 387 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 446
           ++ + L EC EKC  +C+C A+AN+DIR  GSGC IW+G+  DI+     GQDL+VR+ A
Sbjct: 375 DKRIGLKECEEKCKTHCNCTAYANSDIRNGGSGCIIWIGEFRDIRNYAADGQDLFVRLAA 434

Query: 447 SELDIKKA-SSVVIGVIVSATVLIIAGLLLVVFYIIKSRK-------------------- 506
           +E   ++     +IG+I+  +++++   ++  F+  K ++                    
Sbjct: 435 AEFGERRTIRGKIIGLIIGISLMLVLSFIIYCFWKKKQKRARATAAPIGYRDRIQELIIT 494

Query: 507 ----ILKGKELEGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEI 565
               +  G+ L G+EEDLELPL +  T+  AT+NFS+SN LG+GGFG V++GRL DGQEI
Sbjct: 495 NGVVMSSGRRLLGEEEDLELPLTEFETVVMATENFSDSNILGRGGFGIVYKGRLLDGQEI 554


HSP 2 Score: 82.4 bits (202), Expect = 2.1e-14
Identity = 48/111 (43.24%), Postives = 67/111 (60.36%), Query Frame = 0

Query: 566 WKLWKEGIPLELIDPSIGDS------YALSEVLRCIHVSLLCLQQHPEDRPIMSNVILML 625
           W+ WKEG  LE++D  I DS      +   EVLRCI + LLC+Q+  EDRP MS+V+LML
Sbjct: 743 WENWKEGKGLEIVDSIIVDSSSSMSLFQPHEVLRCIQIGLLCVQERAEDRPKMSSVVLML 802

Query: 626 SSET-VLAQPKQPIYYMERDSF----KEDSLSGKNECSTSNELTVTLVHAR 666
            SE   + QPK+P Y + R S        S    +E  T N++TV++++AR
Sbjct: 803 GSEKGEIPQPKRPGYCVGRSSLDTADSSSSTKRDSESLTVNQITVSVINAR 853

BLAST of Spg016816 vs. ExPASy TrEMBL
Match: A0A6J1CD71 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111010408 PE=3 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 3.4e-302
Identity = 545/807 (67.53%), Postives = 586/807 (72.61%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MANF L+S V  TVFL SSFN+SVA DFLTASQNLS+GNTLVSEKG FELGFF PG S N
Sbjct: 1   MANFPLMSIV-TTVFLYSSFNISVAVDFLTASQNLSDGNTLVSEKGFFELGFFSPGNSKN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVE 138
            YLGIWYK IPI TVVWVANRE PL +SSGIL INTTA  IVL QN T+VWS KSLK V 
Sbjct: 61  RYLGIWYKIIPISTVVWVANRENPLNNSSGILRINTTANYIVLTQNSTIVWSTKSLKQVG 120

Query: 139 SPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDD 198
           +PRLQLLD GNLVL DGNS EFLWQSFD+PTDTLLPGMKLGWDFKNG+NRRLS+WK SDD
Sbjct: 121 NPRLQLLDNGNLVLKDGNSREFLWQSFDYPTDTLLPGMKLGWDFKNGINRRLSAWKNSDD 180

Query: 199 PSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEEL 258
           PSPGTL MEMENHSYPE AMWNGTQEF+RTGPWNG+R+S KS +GLPILVYHYVNNK EL
Sbjct: 181 PSPGTLNMEMENHSYPEPAMWNGTQEFMRTGPWNGIRYSSKSNSGLPILVYHYVNNKNEL 240

Query: 259 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 318
           YFSYQLINNSLIGRM LNQS FRRE +LWSE EKNWKVYAT+PRD CD YN CGA+G+C+
Sbjct: 241 YFSYQLINNSLIGRMVLNQSMFRREAILWSEAEKNWKVYATMPRDYCDTYNVCGAYGSCN 300

Query: 319 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 378
           IE MP+CQCLKGFRPRV EKWN+MDYTEGCVRNKPLNCSD+VGFA+ PGLKLPDT+ SWV
Sbjct: 301 IEDMPSCQCLKGFRPRVLEKWNLMDYTEGCVRNKPLNCSDKVGFAKFPGLKLPDTRLSWV 360

Query: 379 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 438
           NESMSL+ECREKCLRNCSCMAFANTDIR SGSGCAIWLGDLIDIKVVLKGGQDLYVR+LA
Sbjct: 361 NESMSLSECREKCLRNCSCMAFANTDIRESGSGCAIWLGDLIDIKVVLKGGQDLYVRVLA 420

Query: 439 SELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDL 498
           SELD K+ASSVVIG+IVSA VLIIAGLLLV FYII+SRK L+GKELEGQEEDL+LPLFDL
Sbjct: 421 SELDKKEASSVVIGIIVSAAVLIIAGLLLVGFYIIRSRKSLEGKELEGQEEDLDLPLFDL 480

Query: 499 STISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAK 558
           S ISNATDNFSN NKLG+GGFGAVFRGRLTDGQEIAVKRLS+YS+QG NEFKNEVILIAK
Sbjct: 481 SAISNATDNFSNCNKLGEGGFGAVFRGRLTDGQEIAVKRLSSYSKQGANEFKNEVILIAK 540

Query: 559 LQHRNL------------------------------------------------------ 618
           LQHRNL                                                      
Sbjct: 541 LQHRNLVKLLGCCIHEKERMLIYEYMPNKSLDSFIFDSAGRNLLDWSKRFNIICGIARGI 600

Query: 619 ------------------------------------------------------------ 666
                                                                       
Sbjct: 601 LYLHQDSRLRIIHRDLKPSNVLLDIDMNPKISDFGMARTFGGDQTEGNTRRVVGTYGYMA 660

BLAST of Spg016816 vs. ExPASy TrEMBL
Match: A0A6J1CE47 (Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=LOC111010408 PE=3 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 1.0e-282
Identity = 514/807 (63.69%), Postives = 551/807 (68.28%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MANF L+S V  TVFL SSFN+SVA DFLTASQNLS+GNTLVSEKG FELGFF PG S N
Sbjct: 1   MANFPLMSIV-TTVFLYSSFNISVAVDFLTASQNLSDGNTLVSEKGFFELGFFSPGNSKN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVE 138
            YLGIWYK IPI TVVWVANRE PL +SSGIL INTTA  IVL QN T+VWS KSLK V 
Sbjct: 61  RYLGIWYKIIPISTVVWVANRENPLNNSSGILRINTTANYIVLTQNSTIVWSTKSLKQVG 120

Query: 139 SPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDD 198
           +PRLQLLD GNLVL DGNS EFLWQSFD+PTDTLLPGMKLGWDFKNG+NRRLS+WK SDD
Sbjct: 121 NPRLQLLDNGNLVLKDGNSREFLWQSFDYPTDTLLPGMKLGWDFKNGINRRLSAWKNSDD 180

Query: 199 PSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEEL 258
           PSPGTL MEMENHSYPE AMWNGTQEF+RTGPWNG+R+S KS +GLPILVYHYVNNK EL
Sbjct: 181 PSPGTLNMEMENHSYPEPAMWNGTQEFMRTGPWNGIRYSSKSNSGLPILVYHYVNNKNEL 240

Query: 259 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 318
           YFSYQLINNSLIGRM LNQS FRRE +LWSE EKNWKVYAT+PRD CD YN CGA+G+C+
Sbjct: 241 YFSYQLINNSLIGRMVLNQSMFRREAILWSEAEKNWKVYATMPRDYCDTYNVCGAYGSCN 300

Query: 319 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 378
           IE MP+CQCLKGFRPRV EKWN+MDYTEGCVRNKPLNCSD+VGFA+ PGLKLPDT+ SWV
Sbjct: 301 IEDMPSCQCLKGFRPRVLEKWNLMDYTEGCVRNKPLNCSDKVGFAKFPGLKLPDTRLSWV 360

Query: 379 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 438
           NESMSL+ECREKCLRNCSCMAFANTDIR SGSGCAIWLGDLIDIKVVLKGGQDLYVR+LA
Sbjct: 361 NESMSLSECREKCLRNCSCMAFANTDIRESGSGCAIWLGDLIDIKVVLKGGQDLYVRVLA 420

Query: 439 SELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDL 498
           SEL                                       GKELEGQEEDL+LPLFDL
Sbjct: 421 SEL---------------------------------------GKELEGQEEDLDLPLFDL 480

Query: 499 STISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAK 558
           S ISNATDNFSN NKLG+GGFGAVFRGRLTDGQEIAVKRLS+YS+QG NEFKNEVILIAK
Sbjct: 481 SAISNATDNFSNCNKLGEGGFGAVFRGRLTDGQEIAVKRLSSYSKQGANEFKNEVILIAK 540

Query: 559 LQHRNL------------------------------------------------------ 618
           LQHRNL                                                      
Sbjct: 541 LQHRNLVKLLGCCIHEKERMLIYEYMPNKSLDSFIFDSAGRNLLDWSKRFNIICGIARGI 600

Query: 619 ------------------------------------------------------------ 666
                                                                       
Sbjct: 601 LYLHQDSRLRIIHRDLKPSNVLLDIDMNPKISDFGMARTFGGDQTEGNTRRVVGTYGYMA 660

BLAST of Spg016816 vs. ExPASy TrEMBL
Match: A0A6J1CFS4 (G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010408 PE=4 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 5.3e-271
Identity = 462/546 (84.62%), Postives = 495/546 (90.66%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MANF L+S V  TVFL SSFN+SVA DFLTASQNLS+GNTLVSEKG FELGFF PG S N
Sbjct: 1   MANFPLMSIV-TTVFLYSSFNISVAVDFLTASQNLSDGNTLVSEKGFFELGFFSPGNSKN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKSLKPVE 138
            YLGIWYK IPI TVVWVANRE PL +SSGIL INTTA  IVL QN T+VWS KSLK V 
Sbjct: 61  RYLGIWYKIIPISTVVWVANRENPLNNSSGILRINTTANYIVLTQNSTIVWSTKSLKQVG 120

Query: 139 SPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDD 198
           +PRLQLLD GNLVL DGNS EFLWQSFD+PTDTLLPGMKLGWDFKNG+NRRLS+WK SDD
Sbjct: 121 NPRLQLLDNGNLVLKDGNSREFLWQSFDYPTDTLLPGMKLGWDFKNGINRRLSAWKNSDD 180

Query: 199 PSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKEEL 258
           PSPGTL MEMENHSYPE AMWNGTQEF+RTGPWNG+R+S KS +GLPILVYHYVNNK EL
Sbjct: 181 PSPGTLNMEMENHSYPEPAMWNGTQEFMRTGPWNGIRYSSKSNSGLPILVYHYVNNKNEL 240

Query: 259 YFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCD 318
           YFSYQLINNSLIGRM LNQS FRRE +LWSE EKNWKVYAT+PRD CD YN CGA+G+C+
Sbjct: 241 YFSYQLINNSLIGRMVLNQSMFRREAILWSEAEKNWKVYATMPRDYCDTYNVCGAYGSCN 300

Query: 319 IETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWV 378
           IE MP+CQCLKGFRPRV EKWN+MDYTEGCVRNKPLNCSD+VGFA+ PGLKLPDT+ SWV
Sbjct: 301 IEDMPSCQCLKGFRPRVLEKWNLMDYTEGCVRNKPLNCSDKVGFAKFPGLKLPDTRLSWV 360

Query: 379 NESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILA 438
           NESMSL+ECREKCLRNCSCMAFANTDIR SGSGCAIWLGDLIDIKVVLKGGQDLYVR+LA
Sbjct: 361 NESMSLSECREKCLRNCSCMAFANTDIRESGSGCAIWLGDLIDIKVVLKGGQDLYVRVLA 420

Query: 439 SELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDL 498
           SELD K+ASSVVIG+IVSA VLIIAGLLLV FYII+SRK L+GKELEGQEEDL+LPLFDL
Sbjct: 421 SELDKKEASSVVIGIIVSAAVLIIAGLLLVGFYIIRSRKSLEGKELEGQEEDLDLPLFDL 480

Query: 499 STISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAK 558
           S ISNATDNFSN NKLG+GGFGAVFRGRLTDGQEIAVKRLS+YS+QG NEFKNEVILIAK
Sbjct: 481 SAISNATDNFSNCNKLGEGGFGAVFRGRLTDGQEIAVKRLSSYSKQGANEFKNEVILIAK 540

Query: 559 LQHRNL 565
           LQHRNL
Sbjct: 541 LQHRNL 545

BLAST of Spg016816 vs. ExPASy TrEMBL
Match: A0A6J1IWF3 (Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111480527 PE=3 SV=1)

HSP 1 Score: 936.4 bits (2419), Expect = 6.5e-269
Identity = 481/818 (58.80%), Postives = 557/818 (68.09%), Query Frame = 0

Query: 9   LAKPNEAQCRMANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFEL 68
           +AKPN  QCRM N  L+SFV  T+FL SSF++S+A DFLT SQ LS+GNTLVSE G FE+
Sbjct: 2   VAKPNGTQCRMGNLPLLSFV-MTLFLYSSFSISLAVDFLTPSQTLSDGNTLVSENGYFEM 61

Query: 69  GFFRPGISNNLYLGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQN-DTV 128
           GFF PG   NLYLGIWYK IPIPTV+WVANRE P+KD  G+L INTTA  I L QN D V
Sbjct: 62  GFFSPGNPMNLYLGIWYKIIPIPTVIWVANREAPIKDLHGVLRINTTASEITLTQNDDLV 121

Query: 129 VWSAKSLKPVESPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMN 188
           VWSAKSLKPV +PRLQLLD+GNLVL DGNSE FLW+SFD+PTDTLLPGMKLGWD+KNG+N
Sbjct: 122 VWSAKSLKPVGNPRLQLLDSGNLVLKDGNSEVFLWESFDYPTDTLLPGMKLGWDYKNGLN 181

Query: 189 RRLSSWKISDDPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPIL 248
           RRLS+WK  DDPSPGTL MEMENHSYPE AMWNGTQEF+R+GPWNG+R+S K     PI 
Sbjct: 182 RRLSAWKNWDDPSPGTLTMEMENHSYPEPAMWNGTQEFMRSGPWNGIRYSAKPFTAFPIF 241

Query: 249 VYHYVNNKEELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDR 308
           V+HY  NK+E+Y+SYQLIN S+IGRM LNQS F+RE LLWSE EKNWKVYA+IPR+ CD 
Sbjct: 242 VFHYTKNKDEVYYSYQLINQSMIGRMVLNQSKFKREALLWSEAEKNWKVYASIPREYCDT 301

Query: 309 YNSCGAFGNCDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPG 368
           YN CGAFG+C+IE+MP+C+CLKGF+P+V E+WN+MDYTEGCVRN+PLNC DEVGFA+  G
Sbjct: 302 YNFCGAFGSCNIESMPSCKCLKGFKPKVAERWNLMDYTEGCVRNRPLNCLDEVGFAKFVG 361

Query: 369 LKLPDTKFSWVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLK 428
           +KLPDTKFSWVNESM L+ECRE CLRNCSCMAFAN DIRGSGSGC IW+GDL+DI V+LK
Sbjct: 362 MKLPDTKFSWVNESMRLDECREMCLRNCSCMAFANXDIRGSGSGCVIWVGDLVDIPVILK 421

Query: 429 GGQDLYVRILASELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQ 488
           GGQDLYV++LASEL+ KK SS+ I V+V A  LI+A LLLV FY+++SRK L+GK+LE Q
Sbjct: 422 GGQDLYVKVLASELETKKKSSLTIVVVVLAAALIVAALLLVGFYVVRSRKHLRGKKLEVQ 481

Query: 489 EEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTN 548
           EE +ELPL+DLSTIS++TDNFSNSNKLG+GGFGAVF GRL +GQEIAVKRLS++SRQG +
Sbjct: 482 EESMELPLYDLSTISHSTDNFSNSNKLGEGGFGAVFWGRLPNGQEIAVKRLSSHSRQGMD 541

Query: 549 EFKNEVILIAKLQHRNL------------------------------------------- 608
           EF NEV LIAKLQHRNL                                           
Sbjct: 542 EFMNEVKLIAKLQHRNLVKLLGCCIQGEEKMLVYEYMPNNSLDSFIFDLAGRKLLDWSKR 601

Query: 609 ------------------------------------------------------------ 666
                                                                       
Sbjct: 602 FNIICGIARGILYLHQDSRLRIIHRDLKPSNILLDVDMIPKISDFGMARTFGGDQIEGNT 661

BLAST of Spg016816 vs. ExPASy TrEMBL
Match: A0A5A7SZX8 (Receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold20G00040 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.3e-266
Identity = 482/814 (59.21%), Postives = 561/814 (68.92%), Query Frame = 0

Query: 19  MANFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNN 78
           MAN SL  F+  ++FL SS   S A DF+T+SQNL++G+TLVS KG FELGFF PG S N
Sbjct: 1   MANASLTLFLFLSLFLFSS--PSTAVDFITSSQNLTHGDTLVSAKGFFELGFFTPGNSTN 60

Query: 79  LYLGIWYKTIPIPTVVWVANRETPLKDSS--GILTINTTAYNIVLIQNDTVVWSAKSLKP 138
            YLGIWYK IP+ T+VWVANRE P+++SS   +L IN+T+ ++ L +ND VVWS KSLKP
Sbjct: 61  RYLGIWYKIIPVRTIVWVANRENPIRNSSAVAVLKINSTSSDLFLFENDVVVWSGKSLKP 120

Query: 139 VESPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKIS 198
            +SP+LQLLD GNLVL D  SEE  WQSFD+PTDTLLPGMKLGWDFKNG+ RRLS+WK S
Sbjct: 121 AKSPKLQLLDDGNLVLKDAESEETSWQSFDYPTDTLLPGMKLGWDFKNGIQRRLSAWKTS 180

Query: 199 DDPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVYHYVNNKE 258
           DDPSPG+L MEM N SYPE AMWNG+ E++R+GPWNG++FS K  + LPILVY YVNNK 
Sbjct: 181 DDPSPGSLTMEMMNTSYPEPAMWNGSNEYMRSGPWNGLQFSAKPTSALPILVYSYVNNKS 240

Query: 259 ELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGN 318
           EL +SY+LIN+SLIGRM LNQ+ FRRE LLWSE+EKNWK+YAT+PRD CD Y  CGAFG+
Sbjct: 241 ELSYSYELINSSLIGRMVLNQTKFRREALLWSESEKNWKLYATMPRDYCDTYGLCGAFGS 300

Query: 319 CDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFS 378
           CDIE +PAC+CL GF P+V EKWN+MDYTEGCVRNKPLNCSD+ GFA+LPGLKLPDTK S
Sbjct: 301 CDIEQVPACRCLFGFHPKVQEKWNLMDYTEGCVRNKPLNCSDKTGFAKLPGLKLPDTKQS 360

Query: 379 WVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRI 438
           WVNESMSLNEC+EKCLR+CSC+AFANTDIRGSG+GCAIW G+L+DIKVV KGGQDLYVR+
Sbjct: 361 WVNESMSLNECKEKCLRDCSCVAFANTDIRGSGNGCAIWFGELVDIKVVRKGGQDLYVRM 420

Query: 439 LASELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRK-----ILKGKELEGQEEDL 498
           LASEL+ KK SS V+G+IV A  L+I GL+L+ FY+I+S++      + GK+LEGQE+DL
Sbjct: 421 LASELETKKTSSAVVGIIVGAAALVILGLVLIGFYVIRSKRRNLQATVAGKDLEGQEDDL 480

Query: 499 ELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKN 558
           ELPLFDL+TISNATDNFSNSNKLG+GGFGAVFRGRL DGQEIAVKRLS+YSRQGT+EFKN
Sbjct: 481 ELPLFDLATISNATDNFSNSNKLGEGGFGAVFRGRLKDGQEIAVKRLSSYSRQGTDEFKN 540

Query: 559 EVILIAKLQHRNL----------------------------------------------- 618
           EVILIAKLQHRNL                                               
Sbjct: 541 EVILIAKLQHRNLVKLLGCCIQGDEKMLIYEYMPNKSLDSFIFDSARKKLLDWSKRFNII 600

Query: 619 ------------------------------------------------------------ 666
                                                                       
Sbjct: 601 CGVARGILYLHQDSRLRIIHRDLKASNVLLDIDLNPKISDFGMARTFGGDQTEGNTRRVV 660

BLAST of Spg016816 vs. TAIR 10
Match: AT4G21380.1 (receptor kinase 3 )

HSP 1 Score: 522.3 bits (1344), Expect = 5.6e-148
Identity = 310/832 (37.26%), Postives = 429/832 (51.56%), Query Frame = 0

Query: 41  SVAADFLTASQNL--SNGNTLVSEKGIFELGFFRPGISNNLYLGIWYKTIPIPTVVWVAN 100
           S++A+ L+AS++L  S+ NT+VS   +FELGFF+PG+ +  YLGIWYK I   T VWVAN
Sbjct: 26  SISANTLSASESLTISSNNTIVSPGNVFELGFFKPGLDSRWYLGIWYKAISKRTYVWVAN 85

Query: 101 RETPLKDSSGILTINTTAYNIVLIQNDTVVWSAK-SLKPVESPRL-QLLDTGNLVLGDGN 160
           R+TPL  S G L I+ +   +VL Q+DT VWS   +   V SP + +LLD GN VL D  
Sbjct: 86  RDTPLSSSIGTLKISDSNL-VVLDQSDTPVWSTNLTGGDVRSPLVAELLDNGNFVLRDSK 145

Query: 161 S---EEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDDPSPGTLAMEMENHSY 220
           +   +  LWQSFD PTDTLLP MKLGWD K G NR + SWK  DDPS G  + ++E   +
Sbjct: 146 NSAPDGVLWQSFDFPTDTLLPEMKLGWDAKTGFNRFIRSWKSPDDPSSGDFSFKLETEGF 205

Query: 221 PELAMWNGTQEFVRTGPWNGVRFSPKSAAGLP------ILVYHYVNNKEELYFSYQLINN 280
           PE+ +WN      R+GPWNG+RFS     G+P       +V+++  +KEE+ +S+++  +
Sbjct: 206 PEIFLWNRESRMYRSGPWNGIRFS-----GVPEMQPFEYMVFNFTTSKEEVTYSFRITKS 265

Query: 281 SLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCDIETMPACQC 340
            +  R++++ S   +    W ET +NW  +   P+D CD Y  CG +G CD  T P C C
Sbjct: 266 DVYSRLSISSSGLLQR-FTWIETAQNWNQFWYAPKDQCDEYKECGVYGYCDSNTSPVCNC 325

Query: 341 LKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFSWVNESMSLNEC 400
           +KGF+PR P+ W + D ++GCVR   L+C    GF +L  +KLPDT  + V+  + + EC
Sbjct: 326 IKGFKPRNPQVWGLRDGSDGCVRKTLLSCGGGDGFVRLKKMKLPDTTTASVDRGIGVKEC 385

Query: 401 REKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILASELDIKK-A 460
            +KCLR+C+C AFANTDIRGSGSGC  W G+L DI+   KGGQDLYVR+ A++L+ K+  
Sbjct: 386 EQKCLRDCNCTAFANTDIRGSGSGCVTWTGELFDIRNYAKGGQDLYVRLAATDLEDKRNR 445

Query: 461 SSVVIGVIVSATVLIIAGLLL-----------------VVFYIIKSRKILKGKEL----- 520
           S+ +IG  +  +VL++   ++                 +V + ++SR +L  + +     
Sbjct: 446 SAKIIGSSIGVSVLLLLSFIIFFLWKRKQKRSILIETPIVDHQLRSRDLLMNEVVISSRR 505

Query: 521 ----EGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSN 580
               E   +DLELPL +   ++ AT+NFSN+NKLG+GGFG V++G+L DGQE+AVKRLS 
Sbjct: 506 HISRENNTDDLELPLMEFEEVAMATNNFSNANKLGQGGFGIVYKGKLLDGQEMAVKRLSK 565

Query: 581 YSRQGTNEFKNEVILIAKLQHRNL------------------------------------ 640
            S QGT+EFKNEV LIA+LQH NL                                    
Sbjct: 566 TSVQGTDEFKNEVKLIARLQHINLVRLLACCVDAGEKMLIYEYLENLSLDSHLFDKSRNS 625

Query: 641 ------------------------------------------------------------ 666
                                                                       
Sbjct: 626 KLNWQMRFDIINGIARGLLYLHQDSRFRIIHRDLKASNILLDKYMTPKISDFGMARIFGR 685

BLAST of Spg016816 vs. TAIR 10
Match: AT4G27290.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 491.1 bits (1263), Expect = 1.4e-138
Identity = 288/804 (35.82%), Postives = 407/804 (50.62%), Query Frame = 0

Query: 32  VFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNNLYLGIWYKTIPIP 91
           + L S+  L+ A D L A+Q L +G+T+VS+ G FE+GFF PG S N YLGIWYK I + 
Sbjct: 12  ISLFSTILLAQATDILIANQTLKDGDTIVSQGGSFEVGFFSPGGSRNRYLGIWYKKISLQ 71

Query: 92  TVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSAKS-----LKPVESPRLQLLD 151
           TVVWVANR++PL D SG L ++      +    + ++WS+ S        + +P +Q+LD
Sbjct: 72  TVVWVANRDSPLYDLSGTLKVSENGSLCLFNDRNHIIWSSSSSPSSQKASLRNPIVQILD 131

Query: 152 TGNLVL-GDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISDDPSPGTLA 211
           TGNLV+   G+ ++++WQS D+P D  LPGMK G +F  G+NR L+SW+  DDPS G   
Sbjct: 132 TGNLVVRNSGDDQDYIWQSLDYPGDMFLPGMKYGLNFVTGLNRFLTSWRAIDDPSTGNYT 191

Query: 212 MEMENHSYPELAMWNGTQEFVRTGPWNGVRFS-PKSAAGLPILVYHYVNNKEELYFSYQL 271
            +M+ +  P+  +   +    RTGPWNG+RF+   +    PI  Y YV  +EE+Y++Y+L
Sbjct: 192 NKMDPNGVPQFFLKKNSVVVFRTGPWNGLRFTGMPNLKPNPIYRYEYVFTEEEVYYTYKL 251

Query: 272 INNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGNCDIETMPA 331
            N S++ RM LN +   +    W +  ++W  Y +   D CD+Y  CG++G+C+I   PA
Sbjct: 252 ENPSVLTRMQLNPNGALQR-YTWVDNLQSWNFYLSAMMDSCDQYTLCGSYGSCNINESPA 311

Query: 332 CQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNC-SDEVGFAQLPGLKLPDTKFSWVNESMS 391
           C+CLKGF  + P+ W   D++EGCVR   L+C   E GF ++  LKLPDT+ SW +++M 
Sbjct: 312 CRCLKGFVAKTPQAWVAGDWSEGCVRRVKLDCGKGEDGFLKISKLKLPDTRTSWYDKNMD 371

Query: 392 LNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRILASELD- 451
           LNEC++ CLRNC+C A++  DIR  G GC +W GDLIDI+   + GQDLYVR+ +SE++ 
Sbjct: 372 LNECKKVCLRNCTCSAYSPFDIRDGGKGCILWFGDLIDIREYNENGQDLYVRLASSEIET 431

Query: 452 IKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFDLSTIS 511
           +++ SS V                        SRK         +EEDLELP  DL T+S
Sbjct: 432 LQRESSRV-----------------------SSRK--------QEEEDLELPFLDLDTVS 491

Query: 512 NATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHR 571
            AT  FS  NKLG+GGFG V++G L  GQE+AVKRLS  SRQG  EFKNE+ LIAKLQHR
Sbjct: 492 EATSGFSAGNKLGQGGFGPVYKGTLACGQEVAVKRLSRTSRQGVEEFKNEIKLIAKLQHR 551

Query: 572 NL---------------------------------------------------------- 631
           NL                                                          
Sbjct: 552 NLVKILGYCVDEEERMLIYEYQPNKSLDSFIFDKERRRELDWPKRVEIIKGIARGMLYLH 611

Query: 632 ------------------------------------------------------------ 666
                                                                       
Sbjct: 612 EDSRLRIIHRDLKASNVLLDSDMNAKISDFGLARTLGGDETEANTTRVVGTYGYMSPEYQ 671

BLAST of Spg016816 vs. TAIR 10
Match: AT1G65790.1 (receptor kinase 1 )

HSP 1 Score: 479.2 bits (1232), Expect = 5.5e-135
Identity = 294/845 (34.79%), Postives = 417/845 (49.35%), Query Frame = 0

Query: 21  NFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNNLY 80
           N+    F+   + L  +F++S      T S  +S+  T++S   IFELGFF P  S+  Y
Sbjct: 6   NYHHSFFIFLILILFLAFSVSPNTLSATESLTISSNKTIISPSQIFELGFFNPASSSRWY 65

Query: 81  LGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLI-QNDTVVWSAK-SLKPVE 140
           LGIWYK IPI T VWVANR+ PL  S+G  T+  +  N+V+  Q+D  VWS   +   V 
Sbjct: 66  LGIWYKIIPIRTYVWVANRDNPLSSSNG--TLKISGNNLVIFDQSDRPVWSTNITGGDVR 125

Query: 141 SP-RLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKISD 200
           SP   +LLD GN +L D N+   LWQSFD PTDTLL  MKLGWD K G NR L SWK +D
Sbjct: 126 SPVAAELLDNGNFLLRDSNN-RLLWQSFDFPTDTLLAEMKLGWDQKTGFNRILRSWKTTD 185

Query: 201 DPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSP-KSAAGLPILVYHYVNNKE 260
           DPS G  + ++E   +PE  + +      R+GPWNG+RFS       +  +VY++  +KE
Sbjct: 186 DPSSGEFSTKLETSEFPEFYICSKESILYRSGPWNGMRFSSVPGTIQVDYMVYNFTASKE 245

Query: 261 ELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSCGAFGN 320
           E+ +SY++   +L  R+ LN +   +  L W ET ++WK     P+D CD Y  CG FG 
Sbjct: 246 EVTYSYRINKTNLYSRLYLNSAGLLQR-LTWFETTQSWKQLWYSPKDLCDNYKVCGNFGY 305

Query: 321 CDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKLPDTKFS 380
           CD  ++P C C+KGF+P   + W++ D + GC+R   L+C    GF +L  +KLPDT  +
Sbjct: 306 CDSNSLPNCYCIKGFKPVNEQAWDLRDGSAGCMRKTRLSCDGRDGFTRLKRMKLPDTTAT 365

Query: 381 WVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQDLYVRI 440
            V+  + L  C+E+CL +C+C AFAN DIR  GSGC IW  +++D++   KGGQDLYVR+
Sbjct: 366 IVDREIGLKVCKERCLEDCNCTAFANADIRNGGSGCVIWTREILDMRNYAKGGQDLYVRL 425

Query: 441 LASELDIKK-ASSVVIGVIVSATVLIIAGLLLVVFY------------------------ 500
            A+EL+ K+  +  +IG  +  ++L++   ++  F+                        
Sbjct: 426 AAAELEDKRIKNEKIIGSSIGVSILLLLSFVIFHFWKRKQKRSITIQTPNVDQVRSQDSL 485

Query: 501 ---IIKSRKILKGKELEGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLT 560
              ++ SR+    K  E + E LELPL +L  ++ AT+NFSN NKLG+GGFG V++GRL 
Sbjct: 486 INDVVVSRRGYTSK--EKKSEYLELPLLELEALATATNNFSNDNKLGQGGFGIVYKGRLL 545

Query: 561 DGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHRNL------------------------ 620
           DG+EIAVKRLS  S QGT+EF NEV LIAKLQH NL                        
Sbjct: 546 DGKEIAVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYLENLS 605

Query: 621 ------------------------------------------------------------ 666
                                                                       
Sbjct: 606 LDSHLFDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKNMTPK 665

BLAST of Spg016816 vs. TAIR 10
Match: AT1G65800.1 (receptor kinase 2 )

HSP 1 Score: 449.5 bits (1155), Expect = 4.6e-126
Identity = 288/849 (33.92%), Postives = 412/849 (48.53%), Query Frame = 0

Query: 21  NFSLISFVAATVFLCSSFNLSVAADFLTASQNLSNGNTLVSEKGIFELGFFRPGISNNLY 80
           N+    F+   + L  +F++  +    T S  +S+  T++S   IFELGFF P  S+  Y
Sbjct: 6   NYHHSYFILFIIILFLAFSVYASNFSATESLTISSNKTIISPSQIFELGFFNPDSSSRWY 65

Query: 81  LGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLI-QNDTVVWSAK-SLKPVE 140
           LGIWYK IPI T VWVANR+ PL  S+G L I+    N+V+  Q+D  VWS   +   V 
Sbjct: 66  LGIWYKIIPIRTYVWVANRDNPLSSSNGTLKISDN--NLVIFDQSDRPVWSTNITGGDVR 125

Query: 141 SP-RLQLLDTGNLVLGDGNSEE---FLWQSFDHPTDTLLPGMKLGWDFKN-GMNRRLSSW 200
           SP   +LLD GN VL D  + +   FLWQSFD PTDTLL  MK+GWD K+ G NR L SW
Sbjct: 126 SPVAAELLDYGNFVLRDSKNNKPSGFLWQSFDFPTDTLLSDMKMGWDNKSGGFNRILRSW 185

Query: 201 KISDDPSPGTLAMEMENHSYPELAMWNGTQEFVRTGPWNGVRFSPKSAAGLPILVY---H 260
           K +DDPS G  + ++    +PE  ++N      R+GPW G RFS  S  G+  + Y    
Sbjct: 186 KTTDDPSSGDFSTKLRTSGFPEFYIYNKESITYRSGPWLGNRFS--SVPGMKPVDYIDNS 245

Query: 261 YVNNKEELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNS 320
           +  N +++ +SY++   ++   ++L+ +   +  L W E  ++WK     P+D CD Y  
Sbjct: 246 FTENNQQVVYSYRVNKTNIYSILSLSSTGLLQR-LTWMEAAQSWKQLWYSPKDLCDNYKE 305

Query: 321 CGAFGNCDIETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLKL 380
           CG +G CD  T P C C+KGF P + E+  + D + GCVR   L+C    GF +L  ++L
Sbjct: 306 CGNYGYCDANTSPICNCIKGFEP-MNEQAALRDDSVGCVRKTKLSCDGRDGFVRLKKMRL 365

Query: 381 PDTKFSWVNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLKGGQ 440
           PDT  + V++ + L EC E+CL+ C+C AFANTDIR  GSGC IW G L DI+   KGGQ
Sbjct: 366 PDTTETSVDKGIGLKECEERCLKGCNCTAFANTDIRNGGSGCVIWSGGLFDIRNYAKGGQ 425

Query: 441 DLYVRILASELDIKK-ASSVVIGVIVSATVLIIAGLLLVVFY----------------II 500
           DLYVR+ A +L+ K+  S  +IG  +  ++L++   ++  F+                ++
Sbjct: 426 DLYVRVAAGDLEDKRIKSKKIIGSSIGVSILLLLSFIIFHFWKRKQKRSITIQTPIVDLV 485

Query: 501 KSRKILKGK---------ELEGQEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFR 560
           +S+  L  +           E + + LELPL +   ++ AT+NFS  NKLG+GGFG V++
Sbjct: 486 RSQDSLMNELVKASRSYTSKENKTDYLELPLMEWKALAMATNNFSTDNKLGQGGFGIVYK 545

Query: 561 GRLTDGQEIAVKRLSNYSRQGTNEFKNEVILIAKLQHRNL-------------------- 620
           G L DG+EIAVKRLS  S QGT+EF NEV LIAKLQH NL                    
Sbjct: 546 GMLLDGKEIAVKRLSKMSSQGTDEFMNEVRLIAKLQHINLVRLLGCCVDKGEKMLIYEYL 605

Query: 621 ------------------------------------------------------------ 666
                                                                       
Sbjct: 606 ENLSLDSHLFDQTRSSNLNWQKRFDIINGIARGLLYLHQDSRCRIIHRDLKASNVLLDKN 665

BLAST of Spg016816 vs. TAIR 10
Match: AT4G27300.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 402.9 bits (1034), Expect = 5.0e-112
Identity = 232/562 (41.28%), Postives = 332/562 (59.07%), Query Frame = 0

Query: 26  SFVAATVFLCSSFNLSVAADF--LTASQNLSNGNTLVSEKGIFELGFF---RPGISNNLY 85
           S  + ++FL SS +LSVA D+  +T  + L +G+TL S   +F+LGFF   +     + +
Sbjct: 6   SLFSLSLFLISS-SLSVALDYNVITPKEFLKDGDTLSSPDQVFQLGFFSLDQEEQPQHRF 65

Query: 86  LGIWYKTIPIPTVVWVANRETPLKDSSGILTINTTAYNIVLIQNDTVVWSA-----KSLK 145
           LG+WY   P   VVWVANR  PL  +SG L +++     +       +WS+     K+ K
Sbjct: 66  LGLWYME-PF-AVVWVANRNNPLYGTSGFLNLSSLGDLQLFDGEHKALWSSSSSSTKASK 125

Query: 146 PVESPRLQLLDTGNLVLGDGNSEEFLWQSFDHPTDTLLPGMKLGWDFKNGMNRRLSSWKI 205
              +P L++  +GNL+  DG  E  LWQSFD+P +T+L GMKLG +FK  M   LSSWK 
Sbjct: 126 TANNPLLKISCSGNLISSDG-EEAVLWQSFDYPMNTILAGMKLGKNFKTQMEWSLSSWKT 185

Query: 206 SDDPSPGTLAMEMENHSYPELAM-WNGTQEF-VRTGPWNGVRFSPKSAAGL--PILVYHY 265
             DPSPG   + ++    P+L +  NG   +  R G WNG+ F+   A G    +  Y +
Sbjct: 186 LKDPSPGDFTLSLDTRGLPQLILRKNGDSSYSYRLGSWNGLSFTGAPAMGRENSLFDYKF 245

Query: 266 VNNKEELYFSYQLINNSLIGRMTLNQSSFRREVLLWSETEKNWKVYATIPRDDCDRYNSC 325
            ++ +E+ +S+    + ++ R+ LN +      +     +  W +  T P D+CD Y+ C
Sbjct: 246 TSSAQEVNYSW-TPRHRIVSRLVLNNTGKLHRFI--QSKQNQWILANTAPEDECDYYSIC 305

Query: 326 GAFGNCDI--ETMPACQCLKGFRPRVPEKWNVMDYTEGCVRNKPLNCSDEVGFAQLPGLK 385
           GA+  C I  +  P+C CL+GF+P+   KWN+     GCV   P NC  +  F + PGLK
Sbjct: 306 GAYAVCGINSKNTPSCSCLQGFKPKSGRKWNISRGAYGCVHEIPTNCEKKDAFVKFPGLK 365

Query: 386 LPDTKFSW--VNESMSLNECREKCLRNCSCMAFANTDIRGSGSGCAIWLGDLIDIKVVLK 445
           LPDT +SW      M+L +C+ KC  NCSC A+ANTDIR  G GC +W GDL+D++    
Sbjct: 366 LPDTSWSWYDAKNEMTLEDCKIKCSSNCSCTAYANTDIREGGKGCLLWFGDLVDMREYSS 425

Query: 446 GGQDLYVRILASELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEG- 505
            GQD+Y+R+  ++++ K     V+G++V + V I   ++LVV +    +KI+K    E  
Sbjct: 426 FGQDVYIRMGFAKIEFK--GREVVGMVVGSVVAI--AVVLVVVFACFRKKIMKRYRGENF 485

Query: 506 ----QEEDLELPLFDLSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQEIAVKRLSNYS 565
               +EEDL+LP+FD  TIS ATD+FS  N LG+GGFG V++G+L DGQEIAVKRLS  S
Sbjct: 486 RKGIEEEDLDLPIFDRKTISIATDDFSYVNFLGRGGFGPVYKGKLEDGQEIAVKRLSANS 545


HSP 2 Score: 76.3 bits (186), Expect = 1.1e-13
Identity = 60/240 (25.00%), Postives = 118/240 (49.17%), Query Frame = 0

Query: 438 ASELDIKKASSVVIGVIVSATVLIIAGLLLVVFYIIKSRKILKGKELEGQEEDLELPLFD 497
           ++ELD KK  +++ GV      L     L ++   +K+  +L   ++  +  D     F 
Sbjct: 591 STELDWKKRMNIINGVARGILYLHQDSRLRIIHRDLKAGNVLLDNDMNPKISD-----FG 650

Query: 498 LSTISNATDNFSNSNKLGKGGFGAVFRGRLTDGQ-----------EIAVKRLSNYSRQGT 557
           L+       + S++N++  G +G +      DG             + ++ ++  + +G 
Sbjct: 651 LAKSFGGDQSESSTNRV-VGTYGYMPPEYAIDGHFSVKSDVFSFGVLVLEIITGKTNRGF 710

Query: 558 NEFKNEVILIAKLQHRNLAWKLWKEGIPLELIDPS-IGDSYALSEVLRCIHVSLLCLQQH 617
               +++ L+  +      WK+W E   +E+ +   + ++  + EVLRCIHV+LLC+QQ 
Sbjct: 711 RHADHDLNLLGHV------WKMWVEDREIEVPEEEWLEETSVIPEVLRCIHVALLCVQQK 770

Query: 618 PEDRPIMSNVILMLSSETVLAQPKQPIYYMERDSFKEDSLSGKNECSTSNELTVTLVHAR 666
           PEDRP M++V+LM  S++ L  P QP ++  R+      +S      + NE+++T++  R
Sbjct: 771 PEDRPTMASVVLMFGSDSSLPHPTQPGFFTNRN---VPDISSSLSLRSQNEVSITMLQGR 815

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139494.17.1e-30267.53G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform ... [more]
XP_038897080.18.9e-29766.10G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 [Beninca... [more]
XP_031738392.14.2e-28664.86G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform ... [more]
XP_022139507.12.1e-28263.69G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform ... [more]
XP_031738391.11.8e-28162.32G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform ... [more]
Match NameE-valueIdentityDescription
O819057.9e-14737.26Receptor-like serine/threonine-protein kinase SD1-8 OS=Arabidopsis thaliana OX=3... [more]
O818322.0e-13735.82G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 OS=Arabi... [more]
Q390867.7e-13434.79Receptor-like serine/threonine-protein kinase SD1-7 OS=Arabidopsis thaliana OX=3... [more]
Q9S9726.5e-12533.92Receptor-like serine/threonine-protein kinase SD1-6 OS=Arabidopsis thaliana OX=3... [more]
P0DH867.2e-12442.56G-type lectin S-receptor-like serine/threonine-protein kinase SRK OS=Arabidopsis... [more]
Match NameE-valueIdentityDescription
A0A6J1CD713.4e-30267.53Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1CE471.0e-28263.69Receptor-like serine/threonine-protein kinase OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1CFS45.3e-27184.62G-type lectin S-receptor-like serine/threonine-protein kinase At4g27290 isoform ... [more]
A0A6J1IWF36.5e-26958.80Receptor-like serine/threonine-protein kinase OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7SZX82.3e-26659.21Receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT4G21380.15.6e-14837.26receptor kinase 3 [more]
AT4G27290.11.4e-13835.82S-locus lectin protein kinase family protein [more]
AT1G65790.15.5e-13534.79receptor kinase 1 [more]
AT1G65800.14.6e-12633.92receptor kinase 2 [more]
AT4G27300.15.0e-11241.28S-locus lectin protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001480Bulb-type lectin domainSMARTSM00108blect_4coord: 50..168
e-value: 1.9E-37
score: 140.4
IPR001480Bulb-type lectin domainPFAMPF01453B_lectincoord: 92..194
e-value: 1.4E-35
score: 121.9
IPR001480Bulb-type lectin domainPROSITEPS50927BULB_LECTINcoord: 44..165
score: 16.08511
IPR001480Bulb-type lectin domainCDDcd00028B_lectincoord: 53..168
e-value: 1.4421E-34
score: 125.502
IPR003609PAN/Apple domainSMARTSM00473ntp_6coord: 357..436
e-value: 4.6E-14
score: 62.7
IPR003609PAN/Apple domainPFAMPF08276PAN_2coord: 356..422
e-value: 1.1E-21
score: 76.7
IPR003609PAN/Apple domainPROSITEPS50948PANcoord: 356..437
score: 11.196589
IPR000858S-locus glycoprotein domainPFAMPF00954S_locus_glycopcoord: 227..334
e-value: 4.6E-26
score: 91.5
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 511..564
e-value: 1.3E-5
score: 24.6
NoneNo IPR availableGENE3D3.50.4.10Hepatocyte Growth Factorcoord: 353..435
e-value: 2.4E-6
score: 29.1
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 473..568
e-value: 8.0E-25
score: 88.8
NoneNo IPR availablePANTHERPTHR27002RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8coord: 565..662
NoneNo IPR availablePANTHERPTHR27002:SF842SUBFAMILY NOT NAMEDcoord: 43..564
NoneNo IPR availablePANTHERPTHR27002RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8coord: 43..564
NoneNo IPR availablePANTHERPTHR27002:SF842SUBFAMILY NOT NAMEDcoord: 565..662
NoneNo IPR availableCDDcd01098PAN_AP_plantcoord: 353..436
e-value: 4.79065E-24
score: 94.4253
IPR036426Bulb-type lectin domain superfamilyGENE3D2.90.10.10coord: 39..166
e-value: 1.1E-18
score: 69.4
IPR036426Bulb-type lectin domain superfamilySUPERFAMILY51110alpha-D-mannose-specific plant lectinscoord: 93..199
IPR021820S-locus receptor kinase, C-terminalPFAMPF11883DUF3403coord: 620..665
e-value: 5.9E-9
score: 35.9
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 514..536
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 508..665
score: 9.941162
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 493..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016816.1Spg016816.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0048544 recognition of pollen
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity