Spg016774 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016774
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionSodium-bile acid cotransporter
Locationscaffold9: 39079860 .. 39082920 (+)
RNA-Seq ExpressionSpg016774
SyntenySpg016774
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGACTCTACCATCGACCACTTCACTCAATATATTATTAACGAACTGACGAGGGTTTTGGCGGGATCCAAGATCGCCGGTCACCGGAGCTCGCCCAGTGCCGGAAATACAGAACTGAAATTCACCACCACTCAGCCATCTAAATTCCTTTATTCTTCTCCGTTTGTTCGTCACTGTGAGAGACGGAGACAATCAGACAATGCGGTCTCTTCCCGTAGATACTTGCGGTCTTCGTCCCCATATTCATCCTCATCAATATTCCCTTTCAAGCTTCAGGACGCGCATTTCACTCGTCACTTCTTCTACTTCTAACAATCCTCTCCCTTCGTCTTTGTCCTCTCCGTCTGCAGCTGGATTTCGCCGCGAATCTTCGCTACGGTTGTGCAACGAACGAAGTTGGGATTGGAATCCGCTTGTATGCTCCAGAACGAGGGCTGATGCCAGATTTCCGGTTCCTCCTCTTCGATGCGGAGCTTCATCGGACGGCAACGGCGTCGGGGGTGAGCGGAGTTTGATGCAGTGGATTGAATTGATCGGCGAAGCTTTGTCGACTGCGTTTCCTGTTTGGGTGGCGTTAGGGTGTTTACTGGGGCTGTTCAGACCGGCTTCCTTCGATTGGGTTCAACCGCGATGGACTGTTCTTGGAATTACTCTCACAATGCTTGGTATGGGTATGACATTGACCCTCGACGATCTTCGTGGAGCCCTGGGAATGCCTAAGGAATTGATTTCTGGATTCTTGCTTCAGTATTCGGTTAGTTCGTTTATTTCTGCTATTATACTGTTGTTTGAACGAATCTGGAACTTATTTTGTTAATCTCGAGTAACATCTTGTGTATGCTGCATAAAATTCTGAATCTGAAAATGAAGAAGGCGGCTGGGGCTGGGAATATTAGAAAGTGGTTTAGAGAAGGGGAAAAAGAAACAAGAGAAGGCTCCAATATATGGATATGAAACAAGGGAAATGTTGAAACAGAAATGCATCAAGTTAATGCTAATACTGGGAAGTTAATCAACTTTTATACTAATTGTCTAATTTCCAAAGTGGGCATGCTTTTTGATATTGATTTGTCAGAATGAGATGAAGATTTACTATTGAACAATACTTGGCTACACACCATGTGTAGCCAAGTATTGTTCTATACTATTTTAAGCTAAATGGATGAATGAATCATTATGTCTTATATGGCATTACTTTCTATGACTTTGAATTGTATGTGCACTATGAAATGGAGAGCGTTTTATTTGAAGGAGAACCTTCCATTATTGCTCTACTGTGTAAATTTTTCTGAGTTTCTAGCCATGCACTTCAATGCTCCTTTCTCCTCTGTATTGAAGTAAACAATTACTAAGAATAGTGAGGCCATGAGGATTTCAGTTTCAGTTTCTTTAGCAGTTCGATGAACATTGATCTATGAATGAAATTCTGATAGATATATTTTCATGATTTCTGTTGCAGGTGATGCCAATATCAGGCTTTGTTGTCAGCAAACTTTTAAATCTGCCATCTTACTATGCAGCTGGTTTAATACTGGTTGGCTGCTGTCCTGGTGGTATGCATCTGTAGGTAGTGTCAATAATCTTTCCCGTCTCGCTATTTTAATAGTGCTGTTTATCTTTGGTTTCAGGAACTGCAAGCAATATCGTGACGTACATTGCACGGTATTCTAATTTTTGAAATGTGATTGCTTAACTCTTTCTTGTTAAATTATCGAAGAGGTTAAGAAATATTTGATGGCCAGGGGAAATGTAGCTCTTTCTGTGCTGATGACAGCGGCAAGCACTGTGGCAGCTGTGGTTAGCAAACCTACATATTACTTTTGCTTTCTTTGTAGAAATGTTTTCATTTTTACATACCTCTTTTGTAATTAAAGCAGAACCTAATATCTCTGTTCGTAGAAATGCAAGCAAAAAAGCAACCATTGTTTACTGCTAATTTCATTTTTACAAGAAATCATATAAAAAGCATCAACTGAACACAACAATAAATTTATTATGTTTTTCTAGTACTCTTCAATCTTTCAGGAATTTAAGCAGACAGTTTCATAGATATTTAAGTCTAAGTTATCATTTCAATGTTACAGATCATGACCCCATTTCTCACTGCTAAGCTTGCTGGTCAATTTGTTGCTGTGGATGCAGCTGGTTTATTAATGTCTACACTGCAGGTGACATTTCTAGCATTCAACCGGGATACCAATTTTTTCTTTTTCCATCTAATTACTACATTACTAACTTCCCTTTCCATCACATGCAGATTGTGCTTCTTCCTGTACTGGCTGGTGCATTTTTAAACCAGTATTTCCACAGCTTGGTTAGACTTGTCTCTCCGTTGATGCCTCCTATTGCTGTTGGTACTGTAGCTATTTTATGTGGAAATGCAATTGCCCAGAGTTCTTCTGCTATTCTCATGTCTGGTCAGCAAGTTCTGCTGGCTGCATCTCTTCTTCACGCTTCAGGATTCTTCTTCGGTTATGTGCTTGCAAGAATGCTTGGTATCGATGTCTCATCTTCTCGGACAATCTCCATCGAAGTTGGAATGCAGGTAAAAACATTTCATTTACCTAAGGCCTATCTTAGCTGCACTTTTTGGCAAGTCTGCTTCTGAATATTTTACCAGATAACTCACTCGTTTCACCGTTCCTGGCTCAACCTGGTTCTAACGAATTGATTTATGTTACAGAACTCGGTACTAGGTGTTGTTCTTGCTACCCAGCACTTTGGAAATCCTCTGACGGCGGTGCCCTGTGCAGTTTCCAGTGTTTGTCACTCAATCTTTGGTAGCGTTCTTGCTGGGATCTGGAGACAACAAAGCAAAGATTAGATAAAAATGATGTCTACATTTTGTATGCGGCATGCAATTGATCTCTCTGATTCAGTAAATTTCAGACATTGGAAGAGCTTATGGGCCATTTCCAAATGTAACCAATTCTTGAGAGGGTGTTGGCAATCATTCCATGTTTGAACTTTGATTAAGTTTAAGTTTGTTGTAGAACGAAGCCCTTGGATGTTATATAAAAAATCTTGCAACTTTTTCTTGGACCTGTTATTATATGGCTTGGG

mRNA sequence

ATGCGGTCTCTTCCCGTAGATACTTGCGGTCTTCGTCCCCATATTCATCCTCATCAATATTCCCTTTCAAGCTTCAGGACGCGCATTTCACTCGTCACTTCTTCTACTTCTAACAATCCTCTCCCTTCGTCTTTGTCCTCTCCGTCTGCAGCTGGATTTCGCCGCGAATCTTCGCTACGGTTGTGCAACGAACGAAGTTGGGATTGGAATCCGCTTGTATGCTCCAGAACGAGGGCTGATGCCAGATTTCCGGTTCCTCCTCTTCGATGCGGAGCTTCATCGGACGGCAACGGCGTCGGGGGTGAGCGGAGTTTGATGCAGTGGATTGAATTGATCGGCGAAGCTTTGTCGACTGCGTTTCCTGTTTGGGTGGCGTTAGGGTGTTTACTGGGGCTGTTCAGACCGGCTTCCTTCGATTGGGTTCAACCGCGATGGACTGTTCTTGGAATTACTCTCACAATGCTTGGTATGGGTATGACATTGACCCTCGACGATCTTCGTGGAGCCCTGGGAATGCCTAAGGAATTGATTTCTGGATTCTTGCTTCAGTATTCGGTGATGCCAATATCAGGCTTTGTTGTCAGCAAACTTTTAAATCTGCCATCTTACTATGCAGCTGGTTTAATACTGGTTGGCTGCTGTCCTGGTGGAACTGCAAGCAATATCGTGACGTACATTGCACGGGGAAATGTAGCTCTTTCTGTGCTGATGACAGCGGCAAGCACTGTGGCAGCTGTGGTTAGCAAACCTACATATTACTTTTGCTTTCTTTGTAGAAATATCATGACCCCATTTCTCACTGCTAAGCTTGCTGGTCAATTTGTTGCTGTGGATGCAGCTGGTTTATTAATGTCTACACTGCAGATTGTGCTTCTTCCTGTACTGGCTGGTGCATTTTTAAACCAGTATTTCCACAGCTTGGTTAGACTTGTCTCTCCGTTGATGCCTCCTATTGCTGTTGGTACTGTAGCTATTTTATGTGGAAATGCAATTGCCCAGAGTTCTTCTGCTATTCTCATGTCTGGTCAGCAAGTTCTGCTGGCTGCATCTCTTCTTCACGCTTCAGGATTCTTCTTCGGTTATGTGCTTGCAAGAATGCTTGGTATCGATGTCTCATCTTCTCGGACAATCTCCATCGAAGTTGGAATGCAGAACTCGGTACTAGGTGTTGTTCTTGCTACCCAGCACTTTGGAAATCCTCTGACGGCGGTGCCCTGTGCAGTTTCCAGTGTTTGTCACTCAATCTTTGGTAGCGTTCTTGCTGGGATCTGGAGACAACAAAGCAAAGATTAG

Coding sequence (CDS)

ATGCGGTCTCTTCCCGTAGATACTTGCGGTCTTCGTCCCCATATTCATCCTCATCAATATTCCCTTTCAAGCTTCAGGACGCGCATTTCACTCGTCACTTCTTCTACTTCTAACAATCCTCTCCCTTCGTCTTTGTCCTCTCCGTCTGCAGCTGGATTTCGCCGCGAATCTTCGCTACGGTTGTGCAACGAACGAAGTTGGGATTGGAATCCGCTTGTATGCTCCAGAACGAGGGCTGATGCCAGATTTCCGGTTCCTCCTCTTCGATGCGGAGCTTCATCGGACGGCAACGGCGTCGGGGGTGAGCGGAGTTTGATGCAGTGGATTGAATTGATCGGCGAAGCTTTGTCGACTGCGTTTCCTGTTTGGGTGGCGTTAGGGTGTTTACTGGGGCTGTTCAGACCGGCTTCCTTCGATTGGGTTCAACCGCGATGGACTGTTCTTGGAATTACTCTCACAATGCTTGGTATGGGTATGACATTGACCCTCGACGATCTTCGTGGAGCCCTGGGAATGCCTAAGGAATTGATTTCTGGATTCTTGCTTCAGTATTCGGTGATGCCAATATCAGGCTTTGTTGTCAGCAAACTTTTAAATCTGCCATCTTACTATGCAGCTGGTTTAATACTGGTTGGCTGCTGTCCTGGTGGAACTGCAAGCAATATCGTGACGTACATTGCACGGGGAAATGTAGCTCTTTCTGTGCTGATGACAGCGGCAAGCACTGTGGCAGCTGTGGTTAGCAAACCTACATATTACTTTTGCTTTCTTTGTAGAAATATCATGACCCCATTTCTCACTGCTAAGCTTGCTGGTCAATTTGTTGCTGTGGATGCAGCTGGTTTATTAATGTCTACACTGCAGATTGTGCTTCTTCCTGTACTGGCTGGTGCATTTTTAAACCAGTATTTCCACAGCTTGGTTAGACTTGTCTCTCCGTTGATGCCTCCTATTGCTGTTGGTACTGTAGCTATTTTATGTGGAAATGCAATTGCCCAGAGTTCTTCTGCTATTCTCATGTCTGGTCAGCAAGTTCTGCTGGCTGCATCTCTTCTTCACGCTTCAGGATTCTTCTTCGGTTATGTGCTTGCAAGAATGCTTGGTATCGATGTCTCATCTTCTCGGACAATCTCCATCGAAGTTGGAATGCAGAACTCGGTACTAGGTGTTGTTCTTGCTACCCAGCACTTTGGAAATCCTCTGACGGCGGTGCCCTGTGCAGTTTCCAGTGTTTGTCACTCAATCTTTGGTAGCGTTCTTGCTGGGATCTGGAGACAACAAAGCAAAGATTAG

Protein sequence

MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLRLCNERSWDWNPLVCSRTRADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALSTAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELISGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQQSKD
Homology
BLAST of Spg016774 vs. NCBI nr
Match: XP_038898003.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida])

HSP 1 Score: 677.2 bits (1746), Expect = 9.5e-191
Identity = 365/431 (84.69%), Postives = 381/431 (88.40%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           M+SLP DT  LRPHI  H+YSLSSFR R SLVTSSTSNNPLPSSLSS S AGF RESSLR
Sbjct: 1   MQSLPSDTFVLRPHIQSHRYSLSSFRRRTSLVTSSTSNNPLPSSLSSSSPAGFHRESSLR 60

Query: 61  LCNERSWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALSTA 120
           L NER W  N   CS+T R D    VPPLRC ASS+GN V GERSLMQWIEL+GEALSTA
Sbjct: 61  LFNERIWSRNAFFCSKTKRIDVGLLVPPLRCEASSNGNSVQGERSLMQWIELLGEALSTA 120

Query: 121 FPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELISG 180
           FPVWVALGCLLGL RPAS+ WVQP WTVLGITLTMLGMGMTLTL+DLRGAL MPKELISG
Sbjct: 121 FPVWVALGCLLGLLRPASYAWVQPGWTVLGITLTMLGMGMTLTLEDLRGALAMPKELISG 180

Query: 181 FLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 240
           FLLQYSVMPISGF+VSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA
Sbjct: 181 FLLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 240

Query: 241 ASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAGAF 300
           AST+AAV              IMTPFLTAKLAGQ+VAVDAAGLLMSTLQIVLLPVL GAF
Sbjct: 241 ASTMAAV--------------IMTPFLTAKLAGQYVAVDAAGLLMSTLQIVLLPVLVGAF 300

Query: 301 LNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGFFF 360
           LNQYFH LV+ VSP MPPIAV TVAILCGNAIAQSSSAILMSGQQV+LAA+LLHASGFFF
Sbjct: 301 LNQYFHGLVKFVSPFMPPIAVSTVAILCGNAIAQSSSAILMSGQQVVLAAALLHASGFFF 360

Query: 361 GYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSV 420
           GYVLAR+LGIDV+SSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHS+FGSV
Sbjct: 361 GYVLARLLGIDVASSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSVFGSV 416

Query: 421 LAGIWRQQSKD 431
           LAGIWR QSKD
Sbjct: 421 LAGIWR-QSKD 416

BLAST of Spg016774 vs. NCBI nr
Match: KAG6607947.1 (putative sodium/metabolite cotransporter BASS1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 660.6 bits (1703), Expect = 9.2e-186
Identity = 361/433 (83.37%), Postives = 377/433 (87.07%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           MRSLP+DT GLRPHI P Q SLSSF  R SL+TSSTSNN      SS SAA F R +SL+
Sbjct: 1   MRSLPLDTYGLRPHIQPPQRSLSSFSPRTSLLTSSTSNN----RFSSASAATFHRNASLQ 60

Query: 61  LCNER--SWDWNPLVCSRTR-ADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALS 120
           + N+R   W+ NPL+CS+T+  D    VPPLRCG+SSD NGV  ERSLMQWIELIGEALS
Sbjct: 61  ISNQRISVWNRNPLLCSKTKWIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALS 120

Query: 121 TAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELI 180
           TAFPVWVALGCLLGL RPAS+DWVQPRWTVLG+T+TMLGMGMTLTLDDLRGAL MPKELI
Sbjct: 121 TAFPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELI 180

Query: 181 SGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240
           SGFLLQYSVMPISG+VVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM
Sbjct: 181 SGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240

Query: 241 TAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAG 300
           TA STVAAV              IMTPFLTAKLAGQFVAVDAAGLL STLQIVLLPVLAG
Sbjct: 241 TAMSTVAAV--------------IMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAG 300

Query: 301 AFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGF 360
           AFLNQYFH LVR VSPLMPPIAVGTVA+LCGNAIAQSSSAIL SG QVLLAASLLH SGF
Sbjct: 301 AFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGF 360

Query: 361 FFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 420
           FFGYVLARMLGID SSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG
Sbjct: 361 FFGYVLARMLGIDTSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 414

Query: 421 SVLAGIWRQQSKD 431
           SVLAGIWR QSKD
Sbjct: 421 SVLAGIWR-QSKD 414

BLAST of Spg016774 vs. NCBI nr
Match: KAG7037462.1 (putative sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659.4 bits (1700), Expect = 2.0e-185
Identity = 361/433 (83.37%), Postives = 376/433 (86.84%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           MRSLP+DT GLRPHI P Q SLSSF  R SL+TSST NN      SS SAA F R +SL+
Sbjct: 18  MRSLPLDTYGLRPHIQPPQRSLSSFSPRTSLLTSSTFNN----RFSSASAATFHRNASLQ 77

Query: 61  LCNER--SWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALS 120
           + N+R   W+ NPL+CS+T R D    VPPLRCG+SSD NGV  ERSLMQWIELIGEALS
Sbjct: 78  ISNQRISVWNRNPLLCSKTKRIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALS 137

Query: 121 TAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELI 180
           TAFPVWVALGCLLGL RPAS+DWVQPRWTVLG+T+TMLGMGMTLTLDDLRGAL MPKELI
Sbjct: 138 TAFPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELI 197

Query: 181 SGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240
           SGFLLQYSVMPISG+VVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM
Sbjct: 198 SGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 257

Query: 241 TAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAG 300
           TA STVAAV              IMTPFLTAKLAGQFVAVDAAGLL STLQIVLLPVLAG
Sbjct: 258 TAMSTVAAV--------------IMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAG 317

Query: 301 AFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGF 360
           AFLNQYFH LVR VSPLMPPIAVGTVA+LCGNAIAQSSSAIL SG QVLLAASLLH SGF
Sbjct: 318 AFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGF 377

Query: 361 FFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 420
           FFGYVLARMLGID SSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG
Sbjct: 378 FFGYVLARMLGIDTSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 431

Query: 421 SVLAGIWRQQSKD 431
           SVLAGIWR QSKD
Sbjct: 438 SVLAGIWR-QSKD 431

BLAST of Spg016774 vs. NCBI nr
Match: XP_022941522.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 659.4 bits (1700), Expect = 2.0e-185
Identity = 361/433 (83.37%), Postives = 376/433 (86.84%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           MRSLP+DT GLRPHI P Q SLSSF  R SL+TSST NN      SS SAA F R +SL+
Sbjct: 1   MRSLPLDTYGLRPHIQPPQRSLSSFSPRTSLLTSSTFNN----RFSSASAATFHRNASLQ 60

Query: 61  LCNER--SWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALS 120
           + N+R   W+ NPL+CS+T R D    VPPLRCG+SSD NGV  ERSLMQWIELIGEALS
Sbjct: 61  ISNQRISVWNRNPLLCSKTKRIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALS 120

Query: 121 TAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELI 180
           TAFPVWVALGCLLGL RPAS+DWVQPRWTVLG+T+TMLGMGMTLTLDDLRGAL MPKELI
Sbjct: 121 TAFPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELI 180

Query: 181 SGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240
           SGFLLQYSVMPISG+VVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM
Sbjct: 181 SGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240

Query: 241 TAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAG 300
           TA STVAAV              IMTPFLTAKLAGQFVAVDAAGLL STLQIVLLPVLAG
Sbjct: 241 TAMSTVAAV--------------IMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAG 300

Query: 301 AFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGF 360
           AFLNQYFH LVR VSPLMPPIAVGTVA+LCGNAIAQSSSAIL SG QVLLAASLLH SGF
Sbjct: 301 AFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGF 360

Query: 361 FFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 420
           FFGYVLARMLGID SSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG
Sbjct: 361 FFGYVLARMLGIDTSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 414

Query: 421 SVLAGIWRQQSKD 431
           SVLAGIWR QSKD
Sbjct: 421 SVLAGIWR-QSKD 414

BLAST of Spg016774 vs. NCBI nr
Match: XP_022982179.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 656.0 bits (1691), Expect = 2.3e-184
Identity = 359/433 (82.91%), Postives = 375/433 (86.61%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           MRSLP+DTC LRPHI P   SLSSF  R SL+TSSTSNN      SS SAA F R +SL+
Sbjct: 1   MRSLPLDTCVLRPHIQPPPRSLSSFSPRTSLLTSSTSNN----RFSSASAATFHRNASLQ 60

Query: 61  LCNER--SWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALS 120
           + NER   W+ NPL+CS+T R D    VPPL+CG+SSD NGV  ERSLMQWIELIGEALS
Sbjct: 61  IFNERISVWNRNPLLCSKTKRIDVGLLVPPLQCGSSSDTNGVQSERSLMQWIELIGEALS 120

Query: 121 TAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELI 180
           TAFPVWVALGCLLGL RPA +DWVQPRWTVLG+T+TMLGMGMTLTLDDLRGAL MPKELI
Sbjct: 121 TAFPVWVALGCLLGLLRPAYYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELI 180

Query: 181 SGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240
           SGFLLQYSVMPISG+VVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM
Sbjct: 181 SGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240

Query: 241 TAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAG 300
           TA STVAAV              IMTPFLTAKLAGQFVAVDAAGLL STLQIVLLPVLAG
Sbjct: 241 TAMSTVAAV--------------IMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAG 300

Query: 301 AFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGF 360
           AFLNQYFH LVR VSPLMPPIAVGTVA+LCGNAIAQSSSAIL SG QVLLAASLLH SGF
Sbjct: 301 AFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGF 360

Query: 361 FFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 420
           FFGYVLARML ID+SSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG
Sbjct: 361 FFGYVLARMLSIDISSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 414

Query: 421 SVLAGIWRQQSKD 431
           SVLAGIWR QSKD
Sbjct: 421 SVLAGIWR-QSKD 414

BLAST of Spg016774 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.2e-140
Identity = 275/409 (67.24%), Postives = 321/409 (78.48%), Query Frame = 0

Query: 21  SLSSFRTRISLVTSSTSNNPL-PSSLSSPSAAGFRRESSLRLCNERSWDWNPLVCSRTRA 80
           SLS       L ++S   + L P  L + S +      S R  + ++    P+ C R+R 
Sbjct: 6   SLSLLNGATPLKSNSLHKSRLTPLHLRTISCSRLSYSPSSREISLKTQSTVPISCRRSRF 65

Query: 81  DARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALSTAFPVWVALGCLLGLFRPASFD 140
           D    VP  RCG SS+      ++S  +W+E +GEA+STAFP+WV+LGCLLGL RP++F+
Sbjct: 66  DF---VP--RCGISSNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFN 125

Query: 141 WVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELISGFLLQYSVMPISGFVVSKLLN 200
           WV P WT++G+T+TMLGMGMTLTLDDLRGAL MPKEL +GFLLQYSVMP+S F VSKLLN
Sbjct: 126 WVTPNWTIVGLTITMLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLN 185

Query: 201 LPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTVAAVVSKPTYYFCFLCR 260
           LP +YAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTV+AV             
Sbjct: 186 LPPHYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTVSAV------------- 245

Query: 261 NIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAGAFLNQYFHSLVRLVSPLMPPIA 320
            IMTP LTAKLA Q++ VDA GLLMSTLQ+VLLPVLAGAFLNQYF  LV+ VSP+MPPIA
Sbjct: 246 -IMTPLLTAKLAKQYITVDALGLLMSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIA 305

Query: 321 VGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGFFFGYVLARMLGIDVSSSRTISI 380
           VGTVAILCG AI Q++SAILMSG+QV+LA+ LLH SGF FGY+ +R+LGIDV+SSRTISI
Sbjct: 306 VGTVAILCGYAIGQNASAILMSGKQVVLASCLLHISGFLFGYLFSRILGIDVASSRTISI 365

Query: 381 EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQQS 429
           EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSI GSVLAGIWR+ +
Sbjct: 366 EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIWRRSA 395

BLAST of Spg016774 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 445.3 bits (1144), Expect = 8.0e-124
Identity = 245/372 (65.86%), Postives = 285/372 (76.61%), Query Frame = 0

Query: 71  PLVCSRTRADARFPV------PPLRCGASSD-------GNGVGG-ERSLMQWIEL---IG 130
           P+ C R R  +  PV      P  RC A++D       G G G  E  ++ W +L   +G
Sbjct: 40  PVTCLRIRYSSSNPVRHLCGIPSSRCHAAADPAPSKIPGGGSGALEAGVVGWRDLLLQVG 99

Query: 131 EALSTAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMP 190
           E LS  FPVWVA  C + L+RP +F WV P   ++GI+ TMLGMGMTLTLDDL+ AL MP
Sbjct: 100 EVLSLGFPVWVASACAVALWRPPAFLWVSPMAQIVGISFTMLGMGMTLTLDDLKTALLMP 159

Query: 191 KELISGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVAL 250
           KEL SGFLLQYSVMP+SGF++SKLLNLPSYYAAGLILV CCPGGTASNIVTY+ARGNVAL
Sbjct: 160 KELASGFLLQYSVMPLSGFLISKLLNLPSYYAAGLILVSCCPGGTASNIVTYLARGNVAL 219

Query: 251 SVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLP 310
           SVLMTAAST AA                +TP LT+KLAGQ+VAVD  GL +ST Q+VL P
Sbjct: 220 SVLMTAASTFAAA--------------FLTPLLTSKLAGQYVAVDPMGLFVSTSQVVLAP 279

Query: 311 VLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLH 370
           VL GA LNQY + LV+LVSPLMP IAV TVA+LCGNAIAQ++SAIL SG QV+++   LH
Sbjct: 280 VLLGALLNQYCNGLVQLVSPLMPFIAVATVAVLCGNAIAQNASAILSSGLQVVMSVCWLH 339

Query: 371 ASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCH 426
           ASGFFFGYVL+R +GID+SSSRTISIEVGMQNSVLGVVLA++HFGNPLTAVPCAVSSVCH
Sbjct: 340 ASGFFFGYVLSRTIGIDISSSRTISIEVGMQNSVLGVVLASKHFGNPLTAVPCAVSSVCH 397

BLAST of Spg016774 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 5.6e-69
Identity = 145/318 (45.60%), Postives = 195/318 (61.32%), Query Frame = 0

Query: 110 ELIGEALSTAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGA 169
           E I E L+T FPVWV LG ++G+++P+   W++     +G+   ML MG+TLT +D R  
Sbjct: 104 EKIVELLTTLFPVWVILGTIIGIYKPSMVTWLETDLFTVGLGFLMLSMGLTLTFEDFRRC 163

Query: 170 LGMPKELISGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARG 229
           +  P  +  GFL QY + P+ GF ++  L L +  A GLILV CCPGG ASN+ TYI++G
Sbjct: 164 MRNPWTVGVGFLAQYLIKPMLGFAIAMTLKLSAPLATGLILVSCCPGGQASNVATYISKG 223

Query: 230 NVALSVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQI 289
           NVALSVLMT  ST+ A+V              MTP LT  LAGQ V VDAAGL +ST Q+
Sbjct: 224 NVALSVLMTTCSTIGAIV--------------MTPLLTKLLAGQLVPVDAAGLAISTFQV 283

Query: 290 VLLPVLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAA 349
           VLLP + G   ++YF      +  + P I V    +LC + I Q S  +   G Q+++  
Sbjct: 284 VLLPTIVGVLAHEYFPKFTERIISITPLIGVLLTTLLCASPIGQVSEVLKAQGGQLIIPV 343

Query: 350 SLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVS 409
           +LLH + F  GY L+++     S+SRTISIE GMQ+S LG +LA +HF NPL AVP AVS
Sbjct: 344 ALLHVAAFALGYWLSKVSSFGESTSRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVS 403

Query: 410 SVCHSIFGSVLAGIWRQQ 428
            VC ++ GS LA  WR +
Sbjct: 404 VVCMALGGSALAVFWRNR 407

BLAST of Spg016774 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.1e-68
Identity = 152/334 (45.51%), Postives = 204/334 (61.08%), Query Frame = 0

Query: 98  GVGGE------RSLMQWIELIGEALSTAFPVWVALGCLLGLFRPASFDWVQPRWTVLGIT 157
           GV G+      + L Q+ ++I E L+T FP+WV LG L+G+F+P+   W++     LG+ 
Sbjct: 78  GVSGDLPESTPKELSQYEKII-ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLFTLGLG 137

Query: 158 LTMLGMGMTLTLDDLRGALGMPKELISGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILV 217
             ML MG+TLT +D R  L  P  +  GFL QY + PI GF+++  L L +  A GLILV
Sbjct: 138 FLMLSMGLTLTFEDFRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILV 197

Query: 218 GCCPGGTASNIVTYIARGNVALSVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLA 277
            CCPGG ASN+ TYI++GNVALSVLMT  ST+ A+              IMTP LT  LA
Sbjct: 198 SCCPGGQASNVATYISKGNVALSVLMTTCSTIGAI--------------IMTPLLTKLLA 257

Query: 278 GQFVAVDAAGLLMSTLQIVLLPVLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAI 337
           GQ V VDAAGL +ST Q+VL+P + G   N++F      +  + P I V    +LC + I
Sbjct: 258 GQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPI 317

Query: 338 AQSSSAILMSGQQVLLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVV 397
            Q +  +   G Q++L  +LLHA+ F  GY +++      S+SRTISIE GMQ+S LG +
Sbjct: 318 GQVADVLKTQGAQLILPVALLHAAAFAIGYWISK-FSFGESTSRTISIECGMQSSALGFL 377

Query: 398 LATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWR 426
           LA +HF NPL AVP AVS VC ++ GS LA  WR
Sbjct: 378 LAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFWR 395

BLAST of Spg016774 vs. ExPASy Swiss-Prot
Match: O34524 (Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 168) OX=224308 GN=yocS PE=3 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.3e-49
Identity = 118/324 (36.42%), Postives = 183/324 (56.48%), Query Frame = 0

Query: 106 MQWIELIGEALSTAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDD 165
           M  I  I       F +WV +  +LG   P+ F W+    T+  + + M GMG+TL  DD
Sbjct: 1   MDIIRKISHFAGQTFGIWVIVFAVLGFSFPSLFTWISSYITIF-LGIIMFGMGLTLQADD 60

Query: 166 LRGALGMPKELISGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTY 225
            +  +  P ++I G + QY++MP+  F ++  L+LP+  A G+ILVGCCPGGTASN++T+
Sbjct: 61  FKELVRKPWQVIIGVIAQYTIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGGTASNVMTF 120

Query: 226 IARGNVALSVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMS 285
           +A+GN ALSV +T  ST+ A V              +TP L    A +++ V    L +S
Sbjct: 121 LAKGNTALSVAVTTISTLLAPV--------------VTPLLIMLFAKEWLPVSPGSLFIS 180

Query: 286 TLQIVLLPVLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQV 345
            LQ VL P++AG  +  +F   V      +P ++V  +  +    ++ +   +L SG  +
Sbjct: 181 ILQAVLFPIIAGLIVKMFFRKQVAKAVHALPLVSVIGIVAIVSAVVSGNRENLLQSGLLI 240

Query: 346 LLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVP 405
                L +  G+  G++ A++L +D  S + I+IEVGMQNS LG  LAT HF +PL+AVP
Sbjct: 241 FSVVILHNGIGYLLGFLCAKLLKMDYPSQKAIAIEVGMQNSGLGAALATAHF-SPLSAVP 300

Query: 406 CAVSSVCHSIFGSVLAGIWRQQSK 430
            A+ SV H++ GS+LA  W ++ K
Sbjct: 301 SAIFSVWHNLSGSMLATYWSKKVK 308

BLAST of Spg016774 vs. ExPASy TrEMBL
Match: A0A6J1FMN4 (probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111446800 PE=3 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 9.9e-186
Identity = 361/433 (83.37%), Postives = 376/433 (86.84%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           MRSLP+DT GLRPHI P Q SLSSF  R SL+TSST NN      SS SAA F R +SL+
Sbjct: 1   MRSLPLDTYGLRPHIQPPQRSLSSFSPRTSLLTSSTFNN----RFSSASAATFHRNASLQ 60

Query: 61  LCNER--SWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALS 120
           + N+R   W+ NPL+CS+T R D    VPPLRCG+SSD NGV  ERSLMQWIELIGEALS
Sbjct: 61  ISNQRISVWNRNPLLCSKTKRIDVGLLVPPLRCGSSSDTNGVQSERSLMQWIELIGEALS 120

Query: 121 TAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELI 180
           TAFPVWVALGCLLGL RPAS+DWVQPRWTVLG+T+TMLGMGMTLTLDDLRGAL MPKELI
Sbjct: 121 TAFPVWVALGCLLGLLRPASYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELI 180

Query: 181 SGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240
           SGFLLQYSVMPISG+VVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM
Sbjct: 181 SGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240

Query: 241 TAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAG 300
           TA STVAAV              IMTPFLTAKLAGQFVAVDAAGLL STLQIVLLPVLAG
Sbjct: 241 TAMSTVAAV--------------IMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAG 300

Query: 301 AFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGF 360
           AFLNQYFH LVR VSPLMPPIAVGTVA+LCGNAIAQSSSAIL SG QVLLAASLLH SGF
Sbjct: 301 AFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGF 360

Query: 361 FFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 420
           FFGYVLARMLGID SSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG
Sbjct: 361 FFGYVLARMLGIDTSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 414

Query: 421 SVLAGIWRQQSKD 431
           SVLAGIWR QSKD
Sbjct: 421 SVLAGIWR-QSKD 414

BLAST of Spg016774 vs. ExPASy TrEMBL
Match: A0A6J1J3V0 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481085 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.1e-184
Identity = 359/433 (82.91%), Postives = 375/433 (86.61%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           MRSLP+DTC LRPHI P   SLSSF  R SL+TSSTSNN      SS SAA F R +SL+
Sbjct: 1   MRSLPLDTCVLRPHIQPPPRSLSSFSPRTSLLTSSTSNN----RFSSASAATFHRNASLQ 60

Query: 61  LCNER--SWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALS 120
           + NER   W+ NPL+CS+T R D    VPPL+CG+SSD NGV  ERSLMQWIELIGEALS
Sbjct: 61  IFNERISVWNRNPLLCSKTKRIDVGLLVPPLQCGSSSDTNGVQSERSLMQWIELIGEALS 120

Query: 121 TAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELI 180
           TAFPVWVALGCLLGL RPA +DWVQPRWTVLG+T+TMLGMGMTLTLDDLRGAL MPKELI
Sbjct: 121 TAFPVWVALGCLLGLLRPAYYDWVQPRWTVLGLTVTMLGMGMTLTLDDLRGALAMPKELI 180

Query: 181 SGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240
           SGFLLQYSVMPISG+VVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM
Sbjct: 181 SGFLLQYSVMPISGYVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240

Query: 241 TAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAG 300
           TA STVAAV              IMTPFLTAKLAGQFVAVDAAGLL STLQIVLLPVLAG
Sbjct: 241 TAMSTVAAV--------------IMTPFLTAKLAGQFVAVDAAGLLRSTLQIVLLPVLAG 300

Query: 301 AFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGF 360
           AFLNQYFH LVR VSPLMPPIAVGTVA+LCGNAIAQSSSAIL SG QVLLAASLLH SGF
Sbjct: 301 AFLNQYFHGLVRFVSPLMPPIAVGTVAVLCGNAIAQSSSAILTSGMQVLLAASLLHMSGF 360

Query: 361 FFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 420
           FFGYVLARML ID+SSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG
Sbjct: 361 FFGYVLARMLSIDISSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 414

Query: 421 SVLAGIWRQQSKD 431
           SVLAGIWR QSKD
Sbjct: 421 SVLAGIWR-QSKD 414

BLAST of Spg016774 vs. ExPASy TrEMBL
Match: A0A5D3DX81 (Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G00270 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.7e-183
Identity = 353/431 (81.90%), Postives = 377/431 (87.47%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           M+SLP DTC LR    P++YSLS+F  R SLVTSS+SNNPL SSLSS S  G RRESSL 
Sbjct: 1   MQSLPSDTCVLR----PYRYSLSTFTPRTSLVTSSSSNNPLNSSLSSFSLVGLRRESSLL 60

Query: 61  LCNERSWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALSTA 120
           L  +R W+ NP VCS+T R D    VPPLRC  SS+GN + GERSLMQWIELIGEALSTA
Sbjct: 61  LFGDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTA 120

Query: 121 FPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELISG 180
           FPVWVALGC+LGL RPAS+ WVQPRWTVLGITLTMLGMGMTLTLDDLRGAL MPKELISG
Sbjct: 121 FPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISG 180

Query: 181 FLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 240
           F+LQYSVMPISGF+VSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA
Sbjct: 181 FVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 240

Query: 241 ASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAGAF 300
            ST+AAV              IMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVL GAF
Sbjct: 241 TSTMAAV--------------IMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLGGAF 300

Query: 301 LNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGFFF 360
           LNQYFH LVR VSP MPPIAVGTVA+LCG+AIAQSSSAI +SGQQV+LAA+LLHASGFFF
Sbjct: 301 LNQYFHGLVRFVSPFMPPIAVGTVAVLCGHAIAQSSSAIRISGQQVVLAAALLHASGFFF 360

Query: 361 GYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSV 420
           GYVLAR+LGID++SSRTISIEVGMQNSVLGVVLA+QHFGNPLTAVPCAVSSVCHSIFGSV
Sbjct: 361 GYVLARLLGIDIASSRTISIEVGMQNSVLGVVLASQHFGNPLTAVPCAVSSVCHSIFGSV 412

Query: 421 LAGIWRQQSKD 431
           LAGIWR QSKD
Sbjct: 421 LAGIWR-QSKD 412

BLAST of Spg016774 vs. ExPASy TrEMBL
Match: A0A1S3CK43 (LOW QUALITY PROTEIN: probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501847 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.7e-183
Identity = 353/431 (81.90%), Postives = 377/431 (87.47%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTSNNPLPSSLSSPSAAGFRRESSLR 60
           M+SLP DTC LR    P++YSLS+F  R SLVTSS+SNNPL SSLSS S  G RRESSL 
Sbjct: 1   MQSLPSDTCVLR----PYRYSLSTFTPRTSLVTSSSSNNPLNSSLSSFSLVGLRRESSLL 60

Query: 61  LCNERSWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALSTA 120
           L  +R W+ NP VCS+T R D    VPPLRC  SS+GN + GERSLMQWIELIGEALSTA
Sbjct: 61  LFGDRIWNRNPFVCSQTKRNDFGLLVPPLRCEPSSNGNSIQGERSLMQWIELIGEALSTA 120

Query: 121 FPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELISG 180
           FPVWVALGC+LGL RPAS+ WVQPRWTVLGITLTMLGMGMTLTLDDLRGAL MPKELISG
Sbjct: 121 FPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELISG 180

Query: 181 FLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 240
           F+LQYSVMPISGF+VSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA
Sbjct: 181 FVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTA 240

Query: 241 ASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAGAF 300
            ST+AAV              IMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVL GAF
Sbjct: 241 TSTMAAV--------------IMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLGGAF 300

Query: 301 LNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGFFF 360
           LNQYFH LVR VSP MPPIAVGTVA+LCG+AIAQSSSAI +SGQQV+LAA+LLHASGFFF
Sbjct: 301 LNQYFHGLVRFVSPFMPPIAVGTVAVLCGHAIAQSSSAIRISGQQVVLAAALLHASGFFF 360

Query: 361 GYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSV 420
           GYVLAR+LGID++SSRTISIEVGMQNSVLGVVLA+QHFGNPLTAVPCAVSSVCHSIFGSV
Sbjct: 361 GYVLARLLGIDIASSRTISIEVGMQNSVLGVVLASQHFGNPLTAVPCAVSSVCHSIFGSV 412

Query: 421 LAGIWRQQSKD 431
           LAGIWR QSKD
Sbjct: 421 LAGIWR-QSKD 412

BLAST of Spg016774 vs. ExPASy TrEMBL
Match: A0A0A0KZF9 (Sodium-bile acid cotransporter OS=Cucumis sativus OX=3659 GN=Csa_4G622840 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 5.6e-181
Identity = 354/433 (81.76%), Postives = 375/433 (86.61%), Query Frame = 0

Query: 1   MRSLPVDTCGLRPHIHPHQYSLSSFRTRISLVTSSTS--NNPLPSSLSSPSAAGFRRESS 60
           M+SLP DTC LR    P++YSLS+FR R SLVTSS+S  N PL SSLSS S  G RRES 
Sbjct: 1   MQSLPSDTCVLR----PYRYSLSTFRPRTSLVTSSSSSFNIPLNSSLSSFSLVGLRRESP 60

Query: 61  LRLCNERSWDWNPLVCSRT-RADARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALS 120
           L L  +  W+ NP VCS+T R D    VPPLRC  SS+GN V GERSLMQWIELIGEALS
Sbjct: 61  LLLFGDPIWNGNPFVCSQTKRRDFGLLVPPLRCEPSSNGNSVQGERSLMQWIELIGEALS 120

Query: 121 TAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELI 180
           TAFPVWVALGC+LGL RPAS+ WVQPRWTVLGITLTMLGMGMTLTLDDLRGAL MPKELI
Sbjct: 121 TAFPVWVALGCVLGLLRPASYAWVQPRWTVLGITLTMLGMGMTLTLDDLRGALAMPKELI 180

Query: 181 SGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240
           SGF+LQYSVMPISGF+VSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM
Sbjct: 181 SGFVLQYSVMPISGFLVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLM 240

Query: 241 TAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAG 300
           TA ST+AAV              IMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVL G
Sbjct: 241 TATSTMAAV--------------IMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLGG 300

Query: 301 AFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGF 360
           AFLNQYFH LVR VSP MPPIAVGTVAILCG+AIAQSSSAI MSGQQV+LAA+LLHASGF
Sbjct: 301 AFLNQYFHGLVRFVSPFMPPIAVGTVAILCGHAIAQSSSAIRMSGQQVVLAAALLHASGF 360

Query: 361 FFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFG 420
           FFGYVLAR+LGID++SSRTISIEVGMQNSVLGVVLA+QHFGNPLTAVPCAVSSVCHSIFG
Sbjct: 361 FFGYVLARLLGIDIASSRTISIEVGMQNSVLGVVLASQHFGNPLTAVPCAVSSVCHSIFG 414

Query: 421 SVLAGIWRQQSKD 431
           SVLAGIWR QSKD
Sbjct: 421 SVLAGIWR-QSKD 414

BLAST of Spg016774 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 501.1 bits (1289), Expect = 8.7e-142
Identity = 275/409 (67.24%), Postives = 321/409 (78.48%), Query Frame = 0

Query: 21  SLSSFRTRISLVTSSTSNNPL-PSSLSSPSAAGFRRESSLRLCNERSWDWNPLVCSRTRA 80
           SLS       L ++S   + L P  L + S +      S R  + ++    P+ C R+R 
Sbjct: 6   SLSLLNGATPLKSNSLHKSRLTPLHLRTISCSRLSYSPSSREISLKTQSTVPISCRRSRF 65

Query: 81  DARFPVPPLRCGASSDGNGVGGERSLMQWIELIGEALSTAFPVWVALGCLLGLFRPASFD 140
           D    VP  RCG SS+      ++S  +W+E +GEA+STAFP+WV+LGCLLGL RP++F+
Sbjct: 66  DF---VP--RCGISSNDLPTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFN 125

Query: 141 WVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKELISGFLLQYSVMPISGFVVSKLLN 200
           WV P WT++G+T+TMLGMGMTLTLDDLRGAL MPKEL +GFLLQYSVMP+S F VSKLLN
Sbjct: 126 WVTPNWTIVGLTITMLGMGMTLTLDDLRGALSMPKELFAGFLLQYSVMPLSAFFVSKLLN 185

Query: 201 LPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTVAAVVSKPTYYFCFLCR 260
           LP +YAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTV+AV             
Sbjct: 186 LPPHYAAGLILVGCCPGGTASNIVTYIARGNVALSVLMTAASTVSAV------------- 245

Query: 261 NIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLAGAFLNQYFHSLVRLVSPLMPPIA 320
            IMTP LTAKLA Q++ VDA GLLMSTLQ+VLLPVLAGAFLNQYF  LV+ VSP+MPPIA
Sbjct: 246 -IMTPLLTAKLAKQYITVDALGLLMSTLQVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIA 305

Query: 321 VGTVAILCGNAIAQSSSAILMSGQQVLLAASLLHASGFFFGYVLARMLGIDVSSSRTISI 380
           VGTVAILCG AI Q++SAILMSG+QV+LA+ LLH SGF FGY+ +R+LGIDV+SSRTISI
Sbjct: 306 VGTVAILCGYAIGQNASAILMSGKQVVLASCLLHISGFLFGYLFSRILGIDVASSRTISI 365

Query: 381 EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQQS 429
           EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSI GSVLAGIWR+ +
Sbjct: 366 EVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIWRRSA 395

BLAST of Spg016774 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-69
Identity = 152/334 (45.51%), Postives = 204/334 (61.08%), Query Frame = 0

Query: 98  GVGGE------RSLMQWIELIGEALSTAFPVWVALGCLLGLFRPASFDWVQPRWTVLGIT 157
           GV G+      + L Q+ ++I E L+T FP+WV LG L+G+F+P+   W++     LG+ 
Sbjct: 78  GVSGDLPESTPKELSQYEKII-ELLTTLFPLWVILGTLVGIFKPSLVTWLETDLFTLGLG 137

Query: 158 LTMLGMGMTLTLDDLRGALGMPKELISGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILV 217
             ML MG+TLT +D R  L  P  +  GFL QY + PI GF+++  L L +  A GLILV
Sbjct: 138 FLMLSMGLTLTFEDFRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSAPLATGLILV 197

Query: 218 GCCPGGTASNIVTYIARGNVALSVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLA 277
            CCPGG ASN+ TYI++GNVALSVLMT  ST+ A+              IMTP LT  LA
Sbjct: 198 SCCPGGQASNVATYISKGNVALSVLMTTCSTIGAI--------------IMTPLLTKLLA 257

Query: 278 GQFVAVDAAGLLMSTLQIVLLPVLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAI 337
           GQ V VDAAGL +ST Q+VL+P + G   N++F      +  + P I V    +LC + I
Sbjct: 258 GQLVPVDAAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLCASPI 317

Query: 338 AQSSSAILMSGQQVLLAASLLHASGFFFGYVLARMLGIDVSSSRTISIEVGMQNSVLGVV 397
            Q +  +   G Q++L  +LLHA+ F  GY +++      S+SRTISIE GMQ+S LG +
Sbjct: 318 GQVADVLKTQGAQLILPVALLHAAAFAIGYWISK-FSFGESTSRTISIECGMQSSALGFL 377

Query: 398 LATQHFGNPLTAVPCAVSSVCHSIFGSVLAGIWR 426
           LA +HF NPL AVP AVS VC ++ GS LA  WR
Sbjct: 378 LAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFWR 395

BLAST of Spg016774 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 154.5 bits (389), Expect = 2.0e-37
Identity = 109/334 (32.63%), Postives = 168/334 (50.30%), Query Frame = 0

Query: 109 IELIGEALSTAF---PVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDD 168
           +  I E L  A+   P  + L  +L L  P SF W +PR+ V G+   M  +G+     D
Sbjct: 88  VSSIMETLKQAYSFIPHGILLSTILALVYPPSFTWFKPRYFVPGLGFMMFAVGINSNERD 147

Query: 169 LRGALGMPKELISGFLLQYSVMPISGFVVS----KLLNLPSYYAAGLILVGCCPGGTASN 228
              AL  P  + +G++ QY + P+ G++       L NLP+   AG++LV C  G   SN
Sbjct: 148 FLEALKRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVSGAQLSN 207

Query: 229 IVTYIARGNV-ALSVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAA 288
             T++   ++ ALS++MT+ ST  AV              ++TP L+  L G+ + VD  
Sbjct: 208 YTTFLTDPSLAALSIVMTSISTATAV--------------LVTPMLSLLLIGKKLPVDVF 267

Query: 289 GLLMSTLQIVLLPVLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILM 348
           G++ S LQ+V+ P+ AG  LN+ F  L   + P +P + V  ++   G  +A +  +IL 
Sbjct: 268 GMISSILQVVITPIAAGLLLNRLFPRLSNAIKPFLPALTVIDMSCCIGAPLALNIDSILS 327

Query: 349 S-GQQVLLAASLLHASGFFFGYVLARML---GIDVSS-SRTISIEVGMQNSVLGVVLATQ 408
             G  +L      H   F  GY           DV +  RTIS E GMQ+S+L + LAT+
Sbjct: 328 PFGATILFLVITFHLLAFVAGYFFTGFFFSKAPDVKALQRTISYETGMQSSLLALALATK 387

Query: 409 HFGNPLTAVPCAVSSVCHSIFGSVLAGIWRQQSK 430
            F +PL  VP A+S+V  S+ G  L  IW+ + +
Sbjct: 388 FFQDPLVGVPPAISTVVMSLMGVSLVTIWKNRKE 407

BLAST of Spg016774 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 149.1 bits (375), Expect = 8.4e-36
Identity = 102/324 (31.48%), Postives = 163/324 (50.31%), Query Frame = 0

Query: 117 STAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKEL 176
           ++  P  V    +L L  P SF W   R+ V  +   M  +G+     D   A   PK +
Sbjct: 98  NSILPHVVLASTILALIYPPSFTWFTSRYFVPALGFLMFAVGINSNEKDFLEAFKRPKAI 157

Query: 177 ISGFLLQYSVMPISGFVVS----KLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVA 236
           + G++ QY V P+ GF+       L  LP+   AG++LV C  G   SN  T++    +A
Sbjct: 158 LLGYVGQYLVKPVLGFIFGLAAVSLFQLPTPIGAGIMLVSCVSGAQLSNYATFLTDPALA 217

Query: 237 -LSVLMTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVL 296
            LS++MT+ ST  AV              ++TP L+  L G+ + VD  G++ S LQ+V+
Sbjct: 218 PLSIVMTSLSTATAV--------------LVTPMLSLLLIGKKLPVDVKGMISSILQVVI 277

Query: 297 LPVLAGAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMS-GQQVLLAAS 356
            P+ AG  LN+ F  +   + P +P ++V   A   G  +A + ++++   G  +LL  +
Sbjct: 278 APIAAGLLLNKLFPKVSNAIRPFLPILSVLDTACCVGAPLALNINSVMSPFGATILLLVT 337

Query: 357 LLHASGFFFGYVLA----RMLGIDVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPC 416
           + H S F  GY L     R      +  RT+S E GMQ+S+L + LAT+ F +PL  +P 
Sbjct: 338 MFHLSAFLAGYFLTGSVFRNAPDAKAMQRTLSYETGMQSSLLALALATKFFQDPLVGIPP 397

Query: 417 AVSSVCHSIFGSVLAGIWRQQSKD 431
           A+S+V  S+ G  L  IW ++  +
Sbjct: 398 AISTVVMSLMGFTLVLIWSKEKSN 407

BLAST of Spg016774 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-35
Identity = 105/311 (33.76%), Postives = 163/311 (52.41%), Query Frame = 0

Query: 117 STAFPVWVALGCLLGLFRPASFDWVQPRWTVLGITLTMLGMGMTLTLDDLRGALGMPKEL 176
           S   P  VAL  +  L  P SF WV        +   ML +G+ L++DD   A   P  L
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWVSKDLYAPALGGIMLSIGIQLSVDDFALAFKRPVPL 171

Query: 177 ISGFLLQYSVMPISGFVVSKLLNLPSYYAAGLILVGCCPGGTASNIVTYIARGNVALSVL 236
             GF+ QY + P+ G +V+    +P  + AG IL  C  G   S+  + +++ +VA+S+L
Sbjct: 172 SVGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVAMSIL 231

Query: 237 MTAASTVAAVVSKPTYYFCFLCRNIMTPFLTAKLAGQFVAVDAAGLLMSTLQIVLLPVLA 296
           +T+++T+A+V              I TP L+  L G  V VDA  +  S LQ+VL+P+  
Sbjct: 232 LTSSTTIASV--------------IFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITL 291

Query: 297 GAFLNQYFHSLVRLVSPLMPPIAVGTVAILCGNAIAQSSSAILMS-GQQVLLAASLLHAS 356
           G  LN Y   +V L+ P+MP +A+   ++  G+ ++ + S IL + G  +++     HA 
Sbjct: 292 GLVLNTYAKPVVTLLQPVMPFVAMVCTSLCIGSPLSINRSQILSAEGLGLIVPIVTFHAV 351

Query: 357 GFFFGYVLARMLGI--DVSSSRTISIEVGMQNSVLGVVLATQHFGNPLTAVPCAVSSVCH 416
            F  GY  +++ G+  +   SRTIS+  GMQ+S L  +LA+Q  G+   AVP A S V  
Sbjct: 352 AFALGYWFSKIPGLRQEEEVSRTISLCTGMQSSTLAGLLASQFLGSS-QAVPAACSVVVM 407

Query: 417 SIFGSVLAGIW 425
           +I G  LA  W
Sbjct: 412 AIMGLCLASFW 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898003.19.5e-19184.69probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida... [more]
KAG6607947.19.2e-18683.37putative sodium/metabolite cotransporter BASS1, chloroplastic, partial [Cucurbit... [more]
KAG7037462.12.0e-18583.37putative sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita argyros... [more]
XP_022941522.12.0e-18583.37probable sodium/metabolite cotransporter BASS1, chloroplastic [Cucurbita moschat... [more]
XP_022982179.12.3e-18482.91probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 [Cucurb... [more]
Match NameE-valueIdentityDescription
Q93YR21.2e-14067.24Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis tha... [more]
Q7XVB38.0e-12465.86Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa su... [more]
Q5VRB25.6e-6945.60Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa su... [more]
Q1EBV72.1e-6845.51Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=37... [more]
O345241.3e-4936.42Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 1... [more]
Match NameE-valueIdentityDescription
A0A6J1FMN49.9e-18683.37probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Cucurbita mosch... [more]
A0A6J1J3V01.1e-18482.91probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 OS=Cucu... [more]
A0A5D3DX812.7e-18381.90Putative sodium/metabolite cotransporter BASS1 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CK432.7e-18381.90LOW QUALITY PROTEIN: probable sodium/metabolite cotransporter BASS1, chloroplast... [more]
A0A0A0KZF95.6e-18181.76Sodium-bile acid cotransporter OS=Cucumis sativus OX=3659 GN=Csa_4G622840 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G78560.18.7e-14267.24Sodium Bile acid symporter family [more]
AT2G26900.11.5e-6945.51Sodium Bile acid symporter family [more]
AT4G12030.22.0e-3732.63bile acid transporter 5 [more]
AT4G22840.18.4e-3631.48Sodium Bile acid symporter family [more]
AT3G25410.11.4e-3533.76Sodium Bile acid symporter family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 103..428
e-value: 3.1E-83
score: 281.4
IPR002657Bile acid:sodium symporter/arsenical resistance protein Acr3PFAMPF01758SBFcoord: 148..334
e-value: 3.9E-33
score: 114.8
IPR004710Bile acid:sodium symporterPANTHERPTHR10361SODIUM-BILE ACID COTRANSPORTERcoord: 86..428
NoneNo IPR availablePANTHERPTHR10361:SF64SODIUM/METABOLITE COTRANSPORTER BASS1, CHLOROPLASTIC-RELATEDcoord: 86..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016774.1Spg016774.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane