Spg016750 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016750 vs. NCBI nr
Match: XP_038896145.1 (amino acid transporter AVT6A-like [Benincasa hispida])

HSP 1 Score: 798.5 bits (2061), Expect = 3.0e-227
Identity = 413/450 (91.78%), Postives = 434/450 (96.44%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +V+ADRK+RRSP+KPLLPEKCDHQE VEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK
Sbjct: 4   LVSADRKYRRSPKKPLLPEKCDHQEAVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGLV+IMLGSTLTELSIDFILKFSR+SKS +YAGAVGDSLG AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLVLIMLGSTLTELSIDFILKFSRASKSVSYAGAVGDSLGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGML+VYMIIIGDVFSGTSANN+HH GVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF
Sbjct: 124 NLGMLVVYMIIIGDVFSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLM+GSIGMPRLMPK+ DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMNGSIGMPRLMPKVTDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           ILVTAYIC HNVHPIENELKDPTQMKSIVRTSLTICS+VYI TSFFGFLLFGDQTLDDVL
Sbjct: 244 ILVTAYICQHNVHPIENELKDPTQMKSIVRTSLTICSTVYITTSFFGFLLFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLN+D LLFPYAIPI+ D++RF
Sbjct: 304 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNIDGLLFPYAIPISFDNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSIT ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDR I
Sbjct: 364 FSITTALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRLI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEKRA 451
           AWIMFLLA+ SS+ AIS DIYS+YVI+K A
Sbjct: 424 AWIMFLLAVLSSIVAISCDIYSSYVIKKHA 453

BLAST of Spg016750 vs. NCBI nr
Match: XP_023535239.1 (amino acid transporter AVT6A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796.2 bits (2055), Expect = 1.5e-226
Identity = 412/450 (91.56%), Postives = 436/450 (96.89%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +VAADRK+RRSPRK LLPEK DHQEP+EVGL+GASFSGAVFNLSSTIVGAGIMALPAAVK
Sbjct: 4   IVAADRKYRRSPRKSLLPEKYDHQEPLEVGLEGASFSGAVFNLSSTIVGAGIMALPAAVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL+MI+LGSTLTELSIDFILKFSR+SKSATYAGAVGDSLG AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLIMIILGSTLTELSIDFILKFSRTSKSATYAGAVGDSLGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGMLIVYMIIIGDVFSGT+ NNVHH GVMEEWFGQHWWTSRISLML TTLLIFAPLISF
Sbjct: 124 NLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRISLMLFTTLLIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP+L+DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELIDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           ILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICSS+YIATSFFGF+LFGDQTLDDVL
Sbjct: 244 ILVTAYICHHNVLPIENELKDPTQMKSIVRTSLIICSSLYIATSFFGFILFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFP AIPIA +++RF
Sbjct: 304 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSIT+ALMSFIF+GANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCG+ASKKDR I
Sbjct: 364 FSITVALMSFIFVGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGVASKKDRVI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEKRA 451
           AW+MFLLA+ SSMAAISSDIYSTYV+EKRA
Sbjct: 424 AWLMFLLAVLSSMAAISSDIYSTYVVEKRA 453

BLAST of Spg016750 vs. NCBI nr
Match: KAG6591444.1 (Amino acid transporter AVT6A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 790.4 bits (2040), Expect = 8.1e-225
Identity = 413/450 (91.78%), Postives = 435/450 (96.67%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +VAADRK+RRSPRK LLPEK DHQEP+E GL+GASFSGAVFNLSSTIVGAGIMALPAAVK
Sbjct: 4   IVAADRKYRRSPRKSLLPEKYDHQEPLE-GLEGASFSGAVFNLSSTIVGAGIMALPAAVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL+MI+LGSTLTELSIDFILKFSR+SKSATYAGAVGDSLG AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLIMIILGSTLTELSIDFILKFSRTSKSATYAGAVGDSLGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGMLIVYMIIIGDVFSGT+ NNVHH GVMEEWFGQHWWTSRISLML TTLLIFAPLISF
Sbjct: 124 NLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRISLMLFTTLLIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP+L+DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELIDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           ILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICSS+YIATSFFGF+LFGDQTLDDVL
Sbjct: 244 ILVTAYICHHNVLPIENELKDPTQMKSIVRTSLIICSSLYIATSFFGFILFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFP AIPIA +++RF
Sbjct: 304 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSIT+ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDR I
Sbjct: 364 FSITVALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRVI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEKRA 451
           AW+MFLLA+ SSMAAISSDIYSTYV+EKRA
Sbjct: 424 AWLMFLLAVLSSMAAISSDIYSTYVVEKRA 452

BLAST of Spg016750 vs. NCBI nr
Match: XP_008452683.1 (PREDICTED: sodium-coupled neutral amino acid transporter 2-like [Cucumis melo] >KAA0064473.1 sodium-coupled neutral amino acid transporter 2-like [Cucumis melo var. makuwa] >TYK20115.1 sodium-coupled neutral amino acid transporter 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 785.4 bits (2027), Expect = 2.6e-223
Identity = 405/448 (90.40%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +V+ADRK+RRSP+KPLLPEK  HQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPA VK
Sbjct: 4   LVSADRKYRRSPKKPLLPEKWGHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAVVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL++IMLGSTLTELSIDFILKFSR+SKS TYAGAVG+S G AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGML+VY+IIIGDV SGTSANN+HH GVMEEWFGQHWWTSRISLMLLTTL IFAPLISF
Sbjct: 124 NLGMLVVYLIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLFIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRL+PK++DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLLPKVIDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           +LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICS+VYIATSFFGFLLFGDQTLDDVL
Sbjct: 244 VLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFPYAIPIA D++RF
Sbjct: 304 ANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPIAFDNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFP ALVLRDTCGIASKKDR I
Sbjct: 364 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPPALVLRDTCGIASKKDRLI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEK 449
           AWIMFLLA+ SS+ AIS DIYS+Y+IEK
Sbjct: 424 AWIMFLLAVFSSIVAISCDIYSSYMIEK 451

BLAST of Spg016750 vs. NCBI nr
Match: XP_022936973.1 (amino acid transporter AVT6A-like [Cucurbita moschata])

HSP 1 Score: 785.4 bits (2027), Expect = 2.6e-223
Identity = 411/450 (91.33%), Postives = 433/450 (96.22%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +VAADRK+RRSPRK LLPEK DHQEP+E GL+GASFSGAVFNLSSTIVGAGIMALPAAVK
Sbjct: 4   IVAADRKYRRSPRKSLLPEKYDHQEPLE-GLEGASFSGAVFNLSSTIVGAGIMALPAAVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL+MI+LGSTLTELSIDFILKFSR+SKSATYAGAVGDSLG AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLLMIILGSTLTELSIDFILKFSRTSKSATYAGAVGDSLGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGMLIVYMIIIGDVFSGT+ NNVHH GVMEEWFGQHWWTSRISLML TTLLIFAPLISF
Sbjct: 124 NLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRISLMLFTTLLIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP+L+DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELIDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           ILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICSS+YIATSFFGF+LFGDQTLDDVL
Sbjct: 244 ILVTAYICHHNVLPIENELKDPTQMKSIVRTSLRICSSLYIATSFFGFILFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFP AIPIA +++RF
Sbjct: 304 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSIT+ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIAS KDR I
Sbjct: 364 FSITVALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASNKDRVI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEKRA 451
           AW+MFLLA+ SS AAISSDIYSTYV+EKRA
Sbjct: 424 AWLMFLLAVLSSTAAISSDIYSTYVVEKRA 452

BLAST of Spg016750 vs. ExPASy Swiss-Prot
Match: Q9LI61 (Amino acid transporter AVT6A OS=Arabidopsis thaliana OX=3702 GN=AVT6A PE=2 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 2.3e-142
Identity = 252/430 (58.60%), Postives = 336/430 (78.14%), Query Frame = 0

Query: 15  PLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLG 74
           PLLPE C   E      +GASFSGAVFNL++TI+GAGIMALPA +K LGL  G+ MI++ 
Sbjct: 25  PLLPE-CHGDEVAHDEFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVM 84

Query: 75  STLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGD 134
           + LT+ SI+F+L+FS++ K+ +Y G +G S G  GR LLQ  ++VNN+G+LIVYMIIIGD
Sbjct: 85  AFLTDASIEFLLRFSKAGKNRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIGD 144

Query: 135 VFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSV 194
           V +G + + +HH GV+E WFG HWW  R +++L+TTL +FAPL  FKR+DSL++TSALSV
Sbjct: 145 VLAGKTEDGIHHFGVLEGWFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSV 204

Query: 195 GLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHP 254
            LA+VF+ ITAG++I KL+ G + MPRL+P + D  SFW LFT VP+LVTA+ICH+NVH 
Sbjct: 205 ALAVVFLIITAGISIMKLISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHS 264

Query: 255 IENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSL 314
           I+NEL+DP+Q++ +VR++L +CSSVYI TS FGFLLFGD TLDDVLANFD DLG+P+ S+
Sbjct: 265 IQNELEDPSQIRPVVRSALMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSI 324

Query: 315 LDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSFIFIG 374
           L+D VRVSY +HLMLVFPIVF+ LR+N+D LLFP A  ++  + RF  +T  L+S IF+G
Sbjct: 325 LNDAVRVSYALHLMLVFPIVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLG 384

Query: 375 ANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAISSSMA 434
           ANF+PSIWDAFQ TGATAA+ +GFIFPA+++L+D    A+ +D T+A  M +LA+ S+  
Sbjct: 385 ANFIPSIWDAFQFTGATAAVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAI 444

Query: 435 AISSDIYSTY 445
           AI SD Y+ +
Sbjct: 445 AIYSDAYALF 453

BLAST of Spg016750 vs. ExPASy Swiss-Prot
Match: F4KBM7 (Amino acid transporter AVT6B OS=Arabidopsis thaliana OX=3702 GN=AVT6B PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 7.3e-136
Identity = 242/415 (58.31%), Postives = 324/415 (78.07%), Query Frame = 0

Query: 32  DGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLGSTLTELSIDFILKFSRS 91
           +GASFSGAVFNL++TI+GAGIMALPA +K LGL+PG+ +I+L + LT+ SI+F+L+FS  
Sbjct: 33  NGASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFSNI 92

Query: 92  SKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGDVFSGTSANNVHHTGVME 151
               +Y G + DS G  GR +LQ  I+V+N+G+LIVYMIIIGDV +G +   +HH G++E
Sbjct: 93  GNQRSYGGVMDDSFGKCGRIMLQVSILVSNIGVLIVYMIIIGDVLAGKNEYGIHHAGMLE 152

Query: 152 EWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAK 211
            WFG  WW  R  ++L+TTL +FAPL  FKR+DSLR+TSA+SV LA+VF+ ITAG+ I K
Sbjct: 153 GWFGISWWNRRTFVLLVTTLTVFAPLTCFKRIDSLRFTSAISVALAVVFLVITAGITIIK 212

Query: 212 LMDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHPIENELKDPTQMKSIVRT 271
           L    + MPRL+P + D +SFW+LFT VP+LV AYICH+NVH I+NEL+DP+++K +VR+
Sbjct: 213 LFTDGLMMPRLLPNVTDLSSFWKLFTVVPVLVNAYICHYNVHSIQNELEDPSRIKPVVRS 272

Query: 272 SLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVF 331
           +L +CSSVY+ TS FG+LLFGD TLDDVLANFD DLG+P+ S+L+D VR SY  HLMLVF
Sbjct: 273 ALAMCSSVYVMTSLFGYLLFGDGTLDDVLANFDTDLGIPFGSVLNDAVRFSYAAHLMLVF 332

Query: 332 PIVFFSLRLNVDSLLFPYAIPI--ALDDQRFFSITIALMSFIFIGANFVPSIWDAFQLTG 391
           P+VF+ LR+N+D L+FP A P+  +  D RF SIT  L++ IF+GANF+PSIWDAFQ TG
Sbjct: 333 PVVFYPLRINIDGLIFPTAPPLTSSESDLRFGSITAGLIAVIFLGANFIPSIWDAFQFTG 392

Query: 392 ATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAISSSMAAISSDIYSTY 445
           ATAA+ +GFIFPAA++L+D    A+K+D+TIA  M +LA+ S+  AI SD Y+ +
Sbjct: 393 ATAAVCIGFIFPAAVILKDRHNQATKRDKTIAICMIVLAVFSNAIAIYSDAYALF 447

BLAST of Spg016750 vs. ExPASy Swiss-Prot
Match: Q9LYM2 (Amino acid transporter AVT6C OS=Arabidopsis thaliana OX=3702 GN=AVT6C PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 5.4e-99
Identity = 190/427 (44.50%), Postives = 287/427 (67.21%), Query Frame = 0

Query: 16  LLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLGS 75
           LLP+    QEP      G+S SG VFN+S++I+GAGIM++PAA K LG++P  ++I + +
Sbjct: 9   LLPK----QEPSSSENHGSSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIA 68

Query: 76  TLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGDV 135
            L+ +S+ F++K + + +S TYAG + +S G  G   +Q   +V   G +I++ IIIGDV
Sbjct: 69  WLSTISVGFLMKSTLAGESTTYAGVMKESFGKTGSIAVQIATMVATFGCMIIFSIIIGDV 128

Query: 136 FSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVG 195
            SG       H GV++EWFG +WW +RI  +L     +  PL+  +RV+ L  +SA+S  
Sbjct: 129 LSGNENGGPEHIGVLQEWFGSYWWNTRIFALLFVYGFVLLPLVLRRRVERLAISSAVSFL 188

Query: 196 LAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHPI 255
           LA++FV I++ +AI+ L++G    PRL P+L +  SFWQLFT  P++VTA+  H NVHPI
Sbjct: 189 LAVLFVVISSVLAISALVNGQTKNPRLFPELSNGGSFWQLFTASPVIVTAFTFHFNVHPI 248

Query: 256 ENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLL 315
             ELKDP Q+    + S+ +C+++Y AT  FG+LLFGD T+ D+L NFD   G    SLL
Sbjct: 249 GFELKDPLQVIPATKISVILCAAIYFATGLFGYLLFGDATMSDILVNFDQSSGSSIGSLL 308

Query: 316 DDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSFIFIGA 375
           +D+VR+SY +HLMLVFP++ FSLR N+D LL+P    +  D +RF  +T+AL+   F+ A
Sbjct: 309 NDIVRLSYVLHLMLVFPLLNFSLRANLDELLYPKKPSLEKDTKRFIGLTLALLICCFLSA 368

Query: 376 NFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAISSSMAA 435
             VP IW  FQ  G+T  +S+ FIFPAA+VLR+  G+++ +++ +A IM +LA+++S+ A
Sbjct: 369 IAVPDIWYFFQFMGSTITVSIAFIFPAAIVLRNIHGVSTSREKIVAAIMLVLAVATSIIA 428

Query: 436 ISSDIYS 443
           IS+++YS
Sbjct: 429 ISTNLYS 431

BLAST of Spg016750 vs. ExPASy Swiss-Prot
Match: Q0WQJ3 (Amino acid transporter AVT6D OS=Arabidopsis thaliana OX=3702 GN=AVT6D PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 7.1e-99
Identity = 194/438 (44.29%), Postives = 294/438 (67.12%), Query Frame = 0

Query: 13  RKPLLPEKCDHQEPVEVGLDG-ASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMI 72
           + PLLP    +QEP     +   SF+GAVFN+S++IVGAGIMA+PAA K LG++P L +I
Sbjct: 6   KAPLLP----NQEPSSSSSENHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLSII 65

Query: 73  MLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMII 132
           ++ + L+ +S  F++K S + +S TYAG + +S G +G   +    +V   G +I++ II
Sbjct: 66  VIIAWLSNVSAGFLMKSSLAGESTTYAGVMKESFGKSGAVAVTVVTMVVTFGSMIIFSII 125

Query: 133 IGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSA 192
           IGDV SG   + + H G+++EWFG HWW +R   +L   + +F PL+  +RV+ L ++SA
Sbjct: 126 IGDVISGNEKDGIIHLGLLQEWFGSHWWNTRFFGLLFIFVFLFLPLVLCRRVERLAFSSA 185

Query: 193 LSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQA-SFWQLFTTVPILVTAYICHH 252
           +S  LA++FV I++ +AI  L+ G    PRL P+L D   SF+ LFT  P++VTA+  H 
Sbjct: 186 ISFLLALLFVVISSVLAIIALVQGKTKPPRLFPELNDGGLSFFSLFTASPVIVTAFTFHF 245

Query: 253 NVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLP 312
           NVHP+  ELKDP  + S  R S+ +C+++Y AT  F +LLFGD T+ DVL NFD      
Sbjct: 246 NVHPVAFELKDPLNVLSATRISVILCATIYSATGLFCYLLFGDSTMTDVLMNFDQSTSSS 305

Query: 313 YSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSF 372
             SLL+D+VR+SY IHLMLVFP++ FSLR N+D LLFP  + +  D++RFF++T  L+  
Sbjct: 306 VGSLLNDIVRLSYAIHLMLVFPLLNFSLRANLDELLFPMKLSLVEDNKRFFALTFPLLIS 365

Query: 373 IFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAIS 432
            F+GA  +P IW  FQ  G+T+ +S+ FIFPAA+VLR+  G ++ +++ +A +M +LA++
Sbjct: 366 CFLGAIAIPDIWYFFQFLGSTSTVSIAFIFPAAIVLRNVNGFSTLREKIVASVMLVLAVA 425

Query: 433 SSMAAISSDIYSTYVIEK 449
           +S+ AIS++IY+    E+
Sbjct: 426 TSIIAISTNIYTFTATEE 439

BLAST of Spg016750 vs. ExPASy Swiss-Prot
Match: Q9M8L9 (Amino acid transporter AVT6E OS=Arabidopsis thaliana OX=3702 GN=AVT6E PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.7e-90
Identity = 180/414 (43.48%), Postives = 274/414 (66.18%), Query Frame = 0

Query: 33  GASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLGSTLTELSIDFILKFSRSS 92
           G+   GAVFNL+++I+GAGIMALPA +K LGL+ G V+I+L + L+E+S++ +++FS   
Sbjct: 74  GSGIYGAVFNLTTSIIGAGIMALPATMKVLGLVLGFVLIILMALLSEISVELLVRFSVLY 133

Query: 93  KSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGDVFSGTSANNVHHTGVMEE 152
           KS +Y   V  +LG   R L + CI+VNN G+L+VY+II+GDV SG    ++HH GV+++
Sbjct: 134 KSKSYGEVVQFALGKTARVLSEICIIVNNGGVLVVYLIIMGDVMSG----SLHHIGVLDQ 193

Query: 153 WFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAKL 212
           W G  +W  R  L+L+  ++  APL +  ++DSL  TSA SV LA+VFV +   VA  KL
Sbjct: 194 WLGNGFWDHRKVLILIVMVIFLAPLCALNKIDSLSVTSAASVALAVVFVVVCFVVATIKL 253

Query: 213 MDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHPIENEL--KDPTQMKSIVR 272
           ++G+I  PRL P    + +   L   +PI+  AY+CH NV PI NEL  + P +M  + R
Sbjct: 254 IEGTIDPPRLSPDFGSKQAILDLLVVIPIMSNAYVCHFNVQPIYNELEGRSPHKMNRVGR 313

Query: 273 TSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLV 332
            +  IC  VY +T+  G+LLFG  T  D+L NFD DLG+ +SS ++ +VR+ Y +HL+LV
Sbjct: 314 ITTAICVVVYASTAVSGYLLFGKDTESDILTNFDQDLGIRFSSAVNYIVRIGYILHLVLV 373

Query: 333 FPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSFIFIGANFVPSIWDAFQLTGA 392
           FP++ FSLR  V++LLF  + P++   +R   +T+ L++ I+IG+  +P+IW AF+ TGA
Sbjct: 374 FPVIHFSLRETVNTLLFEGSPPLSESKKRSLGLTVVLLALIYIGSTMIPNIWTAFKFTGA 433

Query: 393 TAAISVGFIFPAALVLR--DTCGIASKKDRTIAWIMFLLAISSSMAAISSDIYS 443
           T+A+S+GF FPA + LR        S  +R+++W+M +LA+  S+     +IYS
Sbjct: 434 TSAVSLGFTFPALIALRLGKQSNSLSFVERSVSWLMLILAVVVSIVGTIGNIYS 483

BLAST of Spg016750 vs. ExPASy TrEMBL
Match: A0A5A7V8L7 (Sodium-coupled neutral amino acid transporter 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002400 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.3e-223
Identity = 405/448 (90.40%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +V+ADRK+RRSP+KPLLPEK  HQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPA VK
Sbjct: 4   LVSADRKYRRSPKKPLLPEKWGHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAVVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL++IMLGSTLTELSIDFILKFSR+SKS TYAGAVG+S G AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGML+VY+IIIGDV SGTSANN+HH GVMEEWFGQHWWTSRISLMLLTTL IFAPLISF
Sbjct: 124 NLGMLVVYLIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLFIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRL+PK++DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLLPKVIDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           +LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICS+VYIATSFFGFLLFGDQTLDDVL
Sbjct: 244 VLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFPYAIPIA D++RF
Sbjct: 304 ANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPIAFDNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFP ALVLRDTCGIASKKDR I
Sbjct: 364 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPPALVLRDTCGIASKKDRLI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEK 449
           AWIMFLLA+ SS+ AIS DIYS+Y+IEK
Sbjct: 424 AWIMFLLAVFSSIVAISCDIYSSYMIEK 451

BLAST of Spg016750 vs. ExPASy TrEMBL
Match: A0A6J1FER3 (amino acid transporter AVT6A-like OS=Cucurbita moschata OX=3662 GN=LOC111443403 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.3e-223
Identity = 411/450 (91.33%), Postives = 433/450 (96.22%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +VAADRK+RRSPRK LLPEK DHQEP+E GL+GASFSGAVFNLSSTIVGAGIMALPAAVK
Sbjct: 4   IVAADRKYRRSPRKSLLPEKYDHQEPLE-GLEGASFSGAVFNLSSTIVGAGIMALPAAVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL+MI+LGSTLTELSIDFILKFSR+SKSATYAGAVGDSLG AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLLMIILGSTLTELSIDFILKFSRTSKSATYAGAVGDSLGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGMLIVYMIIIGDVFSGT+ NNVHH GVMEEWFGQHWWTSRISLML TTLLIFAPLISF
Sbjct: 124 NLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRISLMLFTTLLIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP+L+DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELIDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           ILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICSS+YIATSFFGF+LFGDQTLDDVL
Sbjct: 244 ILVTAYICHHNVLPIENELKDPTQMKSIVRTSLRICSSLYIATSFFGFILFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFP AIPIA +++RF
Sbjct: 304 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSIT+ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIAS KDR I
Sbjct: 364 FSITVALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASNKDRVI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEKRA 451
           AW+MFLLA+ SS AAISSDIYSTYV+EKRA
Sbjct: 424 AWLMFLLAVLSSTAAISSDIYSTYVVEKRA 452

BLAST of Spg016750 vs. ExPASy TrEMBL
Match: A0A1S3BTU4 (sodium-coupled neutral amino acid transporter 2-like OS=Cucumis melo OX=3656 GN=LOC103493628 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.3e-223
Identity = 405/448 (90.40%), Postives = 429/448 (95.76%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +V+ADRK+RRSP+KPLLPEK  HQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPA VK
Sbjct: 4   LVSADRKYRRSPKKPLLPEKWGHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAVVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL++IMLGSTLTELSIDFILKFSR+SKS TYAGAVG+S G AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGSAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGML+VY+IIIGDV SGTSANN+HH GVMEEWFGQHWWTSRISLMLLTTL IFAPLISF
Sbjct: 124 NLGMLVVYLIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRISLMLLTTLFIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRL+PK++DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLLPKVIDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           +LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICS+VYIATSFFGFLLFGDQTLDDVL
Sbjct: 244 VLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFPYAIPIA D++RF
Sbjct: 304 ANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPIAFDNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFP ALVLRDTCGIASKKDR I
Sbjct: 364 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPPALVLRDTCGIASKKDRLI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEK 449
           AWIMFLLA+ SS+ AIS DIYS+Y+IEK
Sbjct: 424 AWIMFLLAVFSSIVAISCDIYSSYMIEK 451

BLAST of Spg016750 vs. ExPASy TrEMBL
Match: A0A0A0L004 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G645260 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.1e-222
Identity = 404/448 (90.18%), Postives = 427/448 (95.31%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +V+ADRK+RRSP+K LLPEK DHQEPVE GLDGASFSGAVFNLSSTIVGAGIMALPA VK
Sbjct: 4   LVSADRKYRRSPKKLLLPEKYDHQEPVEAGLDGASFSGAVFNLSSTIVGAGIMALPAVVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGL++IMLGSTLTELSIDFILKFSR+SKS TYAGAVG+S G AGRTLLQ CIVVN
Sbjct: 64  QLGLIPGLILIMLGSTLTELSIDFILKFSRASKSVTYAGAVGESFGNAGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGML+VYMIIIGDV SGTSANN+HH GVMEEWFGQHWWTSR+SLMLLTTL IFAPLISF
Sbjct: 124 NLGMLVVYMIIIGDVLSGTSANNIHHKGVMEEWFGQHWWTSRLSLMLLTTLFIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTSALSV LAIVFVAITAGVAIAKLMDGSI MPRLMPK+VDQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSALSVALAIVFVAITAGVAIAKLMDGSIVMPRLMPKVVDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           +LVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICS+VYIATSFFGFLLFGDQTLDDVL
Sbjct: 244 VLVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSTVYIATSFFGFLLFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLP+SSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFPYAIPI  D++RF
Sbjct: 304 ANFDGDLGLPFSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPYAIPITFDNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           F ITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAAL+LRDTCGIASKKDR I
Sbjct: 364 FLITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALILRDTCGIASKKDRLI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEK 449
           AWIMFLLA+ SS+ AIS DIYS+YV+EK
Sbjct: 424 AWIMFLLAVFSSIVAISCDIYSSYVMEK 451

BLAST of Spg016750 vs. ExPASy TrEMBL
Match: A0A6J1IHA5 (amino acid transporter AVT6A-like OS=Cucurbita maxima OX=3661 GN=LOC111477216 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.0e-222
Identity = 410/450 (91.11%), Postives = 430/450 (95.56%), Query Frame = 0

Query: 1   MVAADRKFRRSPRKPLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVK 60
           +VAADRK+RRSPRK LLPEK  HQEP+E GL+GASFSGAVFNLSSTIVGAGIMALPAAVK
Sbjct: 4   IVAADRKYRRSPRKSLLPEKYAHQEPLE-GLEGASFSGAVFNLSSTIVGAGIMALPAAVK 63

Query: 61  QLGLLPGLVMIMLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVN 120
           QLGL+PGLVMI+LGSTLTELSIDFILKFSR+SKSATYAGAV DSLG  GRTLLQ CIVVN
Sbjct: 64  QLGLIPGLVMIILGSTLTELSIDFILKFSRTSKSATYAGAVSDSLGSVGRTLLQVCIVVN 123

Query: 121 NLGMLIVYMIIIGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISF 180
           NLGMLIVYMIIIGDVFSGT+ NNVHH GVMEEWFGQHWWTSR SLML TTLLIFAPLISF
Sbjct: 124 NLGMLIVYMIIIGDVFSGTTTNNVHHKGVMEEWFGQHWWTSRTSLMLFTTLLIFAPLISF 183

Query: 181 KRVDSLRYTSALSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVP 240
           KRVDSLRYTS+LSVGLAIVFVAITAGV+IAKLMDGSIGMPRLMP+L DQASFWQLFTTVP
Sbjct: 184 KRVDSLRYTSSLSVGLAIVFVAITAGVSIAKLMDGSIGMPRLMPELFDQASFWQLFTTVP 243

Query: 241 ILVTAYICHHNVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVL 300
           ILVTAYICHHNV PIENELKDPTQMKSIVRTSL ICSS+YIATSFFGF+LFGDQTLDDVL
Sbjct: 244 ILVTAYICHHNVLPIENELKDPTQMKSIVRTSLIICSSLYIATSFFGFILFGDQTLDDVL 303

Query: 301 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRF 360
           ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVD LLFP AIPIA +++RF
Sbjct: 304 ANFDGDLGLPYSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDGLLFPCAIPIAFNNRRF 363

Query: 361 FSITIALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTI 420
           FSIT+ALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDR I
Sbjct: 364 FSITVALMSFIFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRVI 423

Query: 421 AWIMFLLAISSSMAAISSDIYSTYVIEKRA 451
           AW+MFLLA+ SSMAAISSDIYSTYV+EKRA
Sbjct: 424 AWLMFLLAVLSSMAAISSDIYSTYVVEKRA 452

BLAST of Spg016750 vs. TAIR 10
Match: AT3G30390.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.7e-143
Identity = 252/430 (58.60%), Postives = 336/430 (78.14%), Query Frame = 0

Query: 15  PLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLG 74
           PLLPE C   E      +GASFSGAVFNL++TI+GAGIMALPA +K LGL  G+ MI++ 
Sbjct: 25  PLLPE-CHGDEVAHDEFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVM 84

Query: 75  STLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGD 134
           + LT+ SI+F+L+FS++ K+ +Y G +G S G  GR LLQ  ++VNN+G+LIVYMIIIGD
Sbjct: 85  AFLTDASIEFLLRFSKAGKNRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIGD 144

Query: 135 VFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSV 194
           V +G + + +HH GV+E WFG HWW  R +++L+TTL +FAPL  FKR+DSL++TSALSV
Sbjct: 145 VLAGKTEDGIHHFGVLEGWFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSV 204

Query: 195 GLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHP 254
            LA+VF+ ITAG++I KL+ G + MPRL+P + D  SFW LFT VP+LVTA+ICH+NVH 
Sbjct: 205 ALAVVFLIITAGISIMKLISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHS 264

Query: 255 IENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSL 314
           I+NEL+DP+Q++ +VR++L +CSSVYI TS FGFLLFGD TLDDVLANFD DLG+P+ S+
Sbjct: 265 IQNELEDPSQIRPVVRSALMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSI 324

Query: 315 LDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSFIFIG 374
           L+D VRVSY +HLMLVFPIVF+ LR+N+D LLFP A  ++  + RF  +T  L+S IF+G
Sbjct: 325 LNDAVRVSYALHLMLVFPIVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLG 384

Query: 375 ANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAISSSMA 434
           ANF+PSIWDAFQ TGATAA+ +GFIFPA+++L+D    A+ +D T+A  M +LA+ S+  
Sbjct: 385 ANFIPSIWDAFQFTGATAAVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAI 444

Query: 435 AISSDIYSTY 445
           AI SD Y+ +
Sbjct: 445 AIYSDAYALF 453

BLAST of Spg016750 vs. TAIR 10
Match: AT3G30390.2 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.7e-143
Identity = 252/430 (58.60%), Postives = 336/430 (78.14%), Query Frame = 0

Query: 15  PLLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLG 74
           PLLPE C   E      +GASFSGAVFNL++TI+GAGIMALPA +K LGL  G+ MI++ 
Sbjct: 25  PLLPE-CHGDEVAHDEFNGASFSGAVFNLATTIIGAGIMALPATMKILGLGLGITMIVVM 84

Query: 75  STLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGD 134
           + LT+ SI+F+L+FS++ K+ +Y G +G S G  GR LLQ  ++VNN+G+LIVYMIIIGD
Sbjct: 85  AFLTDASIEFLLRFSKAGKNRSYGGLMGGSFGNPGRILLQVAVLVNNIGVLIVYMIIIGD 144

Query: 135 VFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSV 194
           V +G + + +HH GV+E WFG HWW  R +++L+TTL +FAPL  FKR+DSL++TSALSV
Sbjct: 145 VLAGKTEDGIHHFGVLEGWFGHHWWNGRAAILLITTLGVFAPLACFKRIDSLKFTSALSV 204

Query: 195 GLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHP 254
            LA+VF+ ITAG++I KL+ G + MPRL+P + D  SFW LFT VP+LVTA+ICH+NVH 
Sbjct: 205 ALAVVFLIITAGISIMKLISGGVAMPRLLPDVTDLTSFWNLFTVVPVLVTAFICHYNVHS 264

Query: 255 IENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSL 314
           I+NEL+DP+Q++ +VR++L +CSSVYI TS FGFLLFGD TLDDVLANFD DLG+P+ S+
Sbjct: 265 IQNELEDPSQIRPVVRSALMLCSSVYIMTSIFGFLLFGDDTLDDVLANFDTDLGIPFGSI 324

Query: 315 LDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSFIFIG 374
           L+D VRVSY +HLMLVFPIVF+ LR+N+D LLFP A  ++  + RF  +T  L+S IF+G
Sbjct: 325 LNDAVRVSYALHLMLVFPIVFYPLRINIDGLLFPSARSLSTSNVRFGCLTAGLISVIFLG 384

Query: 375 ANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAISSSMA 434
           ANF+PSIWDAFQ TGATAA+ +GFIFPA+++L+D    A+ +D T+A  M +LA+ S+  
Sbjct: 385 ANFIPSIWDAFQFTGATAAVCLGFIFPASIILKDRHDKATNRDTTLAIFMIVLAVLSNAI 444

Query: 435 AISSDIYSTY 445
           AI SD Y+ +
Sbjct: 445 AIYSDAYALF 453

BLAST of Spg016750 vs. TAIR 10
Match: AT5G38820.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 5.2e-137
Identity = 242/415 (58.31%), Postives = 324/415 (78.07%), Query Frame = 0

Query: 32  DGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLGSTLTELSIDFILKFSRS 91
           +GASFSGAVFNL++TI+GAGIMALPA +K LGL+PG+ +I+L + LT+ SI+F+L+FS  
Sbjct: 33  NGASFSGAVFNLATTIIGAGIMALPATMKILGLIPGITIIVLMAFLTDASIEFLLRFSNI 92

Query: 92  SKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGDVFSGTSANNVHHTGVME 151
               +Y G + DS G  GR +LQ  I+V+N+G+LIVYMIIIGDV +G +   +HH G++E
Sbjct: 93  GNQRSYGGVMDDSFGKCGRIMLQVSILVSNIGVLIVYMIIIGDVLAGKNEYGIHHAGMLE 152

Query: 152 EWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVGLAIVFVAITAGVAIAK 211
            WFG  WW  R  ++L+TTL +FAPL  FKR+DSLR+TSA+SV LA+VF+ ITAG+ I K
Sbjct: 153 GWFGISWWNRRTFVLLVTTLTVFAPLTCFKRIDSLRFTSAISVALAVVFLVITAGITIIK 212

Query: 212 LMDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHPIENELKDPTQMKSIVRT 271
           L    + MPRL+P + D +SFW+LFT VP+LV AYICH+NVH I+NEL+DP+++K +VR+
Sbjct: 213 LFTDGLMMPRLLPNVTDLSSFWKLFTVVPVLVNAYICHYNVHSIQNELEDPSRIKPVVRS 272

Query: 272 SLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLLDDVVRVSYGIHLMLVF 331
           +L +CSSVY+ TS FG+LLFGD TLDDVLANFD DLG+P+ S+L+D VR SY  HLMLVF
Sbjct: 273 ALAMCSSVYVMTSLFGYLLFGDGTLDDVLANFDTDLGIPFGSVLNDAVRFSYAAHLMLVF 332

Query: 332 PIVFFSLRLNVDSLLFPYAIPI--ALDDQRFFSITIALMSFIFIGANFVPSIWDAFQLTG 391
           P+VF+ LR+N+D L+FP A P+  +  D RF SIT  L++ IF+GANF+PSIWDAFQ TG
Sbjct: 333 PVVFYPLRINIDGLIFPTAPPLTSSESDLRFGSITAGLIAVIFLGANFIPSIWDAFQFTG 392

Query: 392 ATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAISSSMAAISSDIYSTY 445
           ATAA+ +GFIFPAA++L+D    A+K+D+TIA  M +LA+ S+  AI SD Y+ +
Sbjct: 393 ATAAVCIGFIFPAAVILKDRHNQATKRDKTIAICMIVLAVFSNAIAIYSDAYALF 447

BLAST of Spg016750 vs. TAIR 10
Match: AT3G56200.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 362.8 bits (930), Expect = 3.9e-100
Identity = 190/427 (44.50%), Postives = 287/427 (67.21%), Query Frame = 0

Query: 16  LLPEKCDHQEPVEVGLDGASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMIMLGS 75
           LLP+    QEP      G+S SG VFN+S++I+GAGIM++PAA K LG++P  ++I + +
Sbjct: 9   LLPK----QEPSSSENHGSSTSGIVFNVSTSIIGAGIMSMPAAFKVLGIVPAFLIITIIA 68

Query: 76  TLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMIIIGDV 135
            L+ +S+ F++K + + +S TYAG + +S G  G   +Q   +V   G +I++ IIIGDV
Sbjct: 69  WLSTISVGFLMKSTLAGESTTYAGVMKESFGKTGSIAVQIATMVATFGCMIIFSIIIGDV 128

Query: 136 FSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSALSVG 195
            SG       H GV++EWFG +WW +RI  +L     +  PL+  +RV+ L  +SA+S  
Sbjct: 129 LSGNENGGPEHIGVLQEWFGSYWWNTRIFALLFVYGFVLLPLVLRRRVERLAISSAVSFL 188

Query: 196 LAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQASFWQLFTTVPILVTAYICHHNVHPI 255
           LA++FV I++ +AI+ L++G    PRL P+L +  SFWQLFT  P++VTA+  H NVHPI
Sbjct: 189 LAVLFVVISSVLAISALVNGQTKNPRLFPELSNGGSFWQLFTASPVIVTAFTFHFNVHPI 248

Query: 256 ENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLPYSSLL 315
             ELKDP Q+    + S+ +C+++Y AT  FG+LLFGD T+ D+L NFD   G    SLL
Sbjct: 249 GFELKDPLQVIPATKISVILCAAIYFATGLFGYLLFGDATMSDILVNFDQSSGSSIGSLL 308

Query: 316 DDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSFIFIGA 375
           +D+VR+SY +HLMLVFP++ FSLR N+D LL+P    +  D +RF  +T+AL+   F+ A
Sbjct: 309 NDIVRLSYVLHLMLVFPLLNFSLRANLDELLYPKKPSLEKDTKRFIGLTLALLICCFLSA 368

Query: 376 NFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAISSSMAA 435
             VP IW  FQ  G+T  +S+ FIFPAA+VLR+  G+++ +++ +A IM +LA+++S+ A
Sbjct: 369 IAVPDIWYFFQFMGSTITVSIAFIFPAAIVLRNIHGVSTSREKIVAAIMLVLAVATSIIA 428

Query: 436 ISSDIYS 443
           IS+++YS
Sbjct: 429 ISTNLYS 431

BLAST of Spg016750 vs. TAIR 10
Match: AT2G40420.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 362.5 bits (929), Expect = 5.0e-100
Identity = 194/438 (44.29%), Postives = 294/438 (67.12%), Query Frame = 0

Query: 13  RKPLLPEKCDHQEPVEVGLDG-ASFSGAVFNLSSTIVGAGIMALPAAVKQLGLLPGLVMI 72
           + PLLP    +QEP     +   SF+GAVFN+S++IVGAGIMA+PAA K LG++P L +I
Sbjct: 6   KAPLLP----NQEPSSSSSENHGSFAGAVFNISTSIVGAGIMAIPAAFKVLGVIPSLSII 65

Query: 73  MLGSTLTELSIDFILKFSRSSKSATYAGAVGDSLGGAGRTLLQACIVVNNLGMLIVYMII 132
           ++ + L+ +S  F++K S + +S TYAG + +S G +G   +    +V   G +I++ II
Sbjct: 66  VIIAWLSNVSAGFLMKSSLAGESTTYAGVMKESFGKSGAVAVTVVTMVVTFGSMIIFSII 125

Query: 133 IGDVFSGTSANNVHHTGVMEEWFGQHWWTSRISLMLLTTLLIFAPLISFKRVDSLRYTSA 192
           IGDV SG   + + H G+++EWFG HWW +R   +L   + +F PL+  +RV+ L ++SA
Sbjct: 126 IGDVISGNEKDGIIHLGLLQEWFGSHWWNTRFFGLLFIFVFLFLPLVLCRRVERLAFSSA 185

Query: 193 LSVGLAIVFVAITAGVAIAKLMDGSIGMPRLMPKLVDQA-SFWQLFTTVPILVTAYICHH 252
           +S  LA++FV I++ +AI  L+ G    PRL P+L D   SF+ LFT  P++VTA+  H 
Sbjct: 186 ISFLLALLFVVISSVLAIIALVQGKTKPPRLFPELNDGGLSFFSLFTASPVIVTAFTFHF 245

Query: 253 NVHPIENELKDPTQMKSIVRTSLTICSSVYIATSFFGFLLFGDQTLDDVLANFDGDLGLP 312
           NVHP+  ELKDP  + S  R S+ +C+++Y AT  F +LLFGD T+ DVL NFD      
Sbjct: 246 NVHPVAFELKDPLNVLSATRISVILCATIYSATGLFCYLLFGDSTMTDVLMNFDQSTSSS 305

Query: 313 YSSLLDDVVRVSYGIHLMLVFPIVFFSLRLNVDSLLFPYAIPIALDDQRFFSITIALMSF 372
             SLL+D+VR+SY IHLMLVFP++ FSLR N+D LLFP  + +  D++RFF++T  L+  
Sbjct: 306 VGSLLNDIVRLSYAIHLMLVFPLLNFSLRANLDELLFPMKLSLVEDNKRFFALTFPLLIS 365

Query: 373 IFIGANFVPSIWDAFQLTGATAAISVGFIFPAALVLRDTCGIASKKDRTIAWIMFLLAIS 432
            F+GA  +P IW  FQ  G+T+ +S+ FIFPAA+VLR+  G ++ +++ +A +M +LA++
Sbjct: 366 CFLGAIAIPDIWYFFQFLGSTSTVSIAFIFPAAIVLRNVNGFSTLREKIVASVMLVLAVA 425

Query: 433 SSMAAISSDIYSTYVIEK 449
           +S+ AIS++IY+    E+
Sbjct: 426 TSIIAISTNIYTFTATEE 439