Spg016746 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016746
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationscaffold9: 45878218 .. 45880931 (+)
RNA-Seq ExpressionSpg016746
SyntenySpg016746
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAATTATTCATTCCCAATCTTATTTTCCATATTTCCTCACCTTTGAATCCGACATGGCTGCAGCAGATTCCAACGACCCAGAGCTGGATGTCGAGCTTCTTCCTTACCTTAAACTCTACAAAAACGGTGTCGTCGAGAGGCTTATCGGGACACGTGTCACTCCGCCGGGGCTCGATTCTCAAACTGGAGTTGACTCTAAAGATATCGTTATTGTCCCAGAAACTGGCGTCTCGGCTCGACTTTACCGTCCCACCGCCGTCGAACCTCGCCGGAAACTTCCCTTGGTTGTTTACTTTCACGGCGGCGCGTTTGTTATAGCCTCTAGTGCTGAACCTATCTACCATAACAATTGTCTCATCCCGCTCGCTGCTGAGGTTGAAAGCGTCGTCGTTTCGGTGAATTACAGATTAGCGCCGGAGCACCCTCTCCCCGCCGCTTACGACGATTCGTGGGCTGCGCTGCAGTGGATTGCCGCCCAGTCCCAGTCCTCCGGCGACGATGCCGACCATGAGCCTTGGCTTAAACAGTTGGTCGACTTCGAGAAGGTATTGTTCAGTATCCTAGGACTTACACTTTTACAGTAAATTCATTTATTTTACATAGGTGTATATAAAATTCATTTTTTTTAAAAAGTTTAATAACATTTAGAGTAGTCGAACTTTTGAACTTTCAATACTATATGTTAATTAGTGTTGAGTTATGCTCACTTTAACTATAAAATCCATTAATTATTTTAATTTTTATATATGTGTCTATATTTTGAGTTGGACAAGAAGTCACTAACGTACCAAAAAGTGATTCATTTATATTATATTTATCAACATAGGTCTACACGGTTGGATAATAAAGAAAAATTGTAAAAACAACTCGATCTTGTATCCAACAATTATTAGAAGCACACAAATTTAATTTGAAATCTACATCCAACGATTTTAAAGAGCGTAACGATTCCATTGAATAATTAGCAGTGTTTTTTATCCTTTTAAATTAGTAGCATTTAAAAAAATTTATAATAACCACAATTAGTTTGAAAATTTATTATTTTTTCTTTTAAGTCAAGTTTTAAAATTTATAAATGCATATACAAAGTTTTTTTCTAAACCTTTTATATATACTAATTTTCTTTCAAGATTGTTTATATAATAATTGTCTAACTTAAAACCATAATTAATTAATTATTTATTTCTTTTGTATGCTAAACACATTTTTAAGAAACCATCAAGAATGACTCAATTAAGGACTTGTATTTTCGACAAAAAGGTCATAGGTTCGAATCTCTTCACCTCACATACTTAGTACATATTTTATGTTAGTAAATATACGAGCGTAGCTGTTAACATGAAGTATAATTCAACTGACATAAGACTTATACTAATGACTACGAGCTTTTTGATTCGAACCAACATTCTTTTGAAGGTTTAAATTAAATACTTACCTCAGACTATGCTAAAGAGAGAGAGAAAAAAAAAACATTCTAACCCCAAAACTTCCACTATAATACTCATATGTACTAAATTATCAACAGAAACACAAACTTAAGTAAATAAGGGTATGTTGTTTAAAAAAAAAAAAAAAAGTAAATAAGGGTATGTTTGGTTTAATTTTGAAAAAGAATCATTTTGAAAAAAATTATAGTGTTTGACAACCACTAAAAATGACTTATTAAAAAATAAGATTGAAAAATAAGTCATTTTGAGAGAAACATTGAAACTAAAATTTTTAAAAAGAGTTTTTAAGTATATAATAACTCAGCTATAAAAACACTTGGAAGGAATGTAATCAAACTATGAGTGTTTAAATTCTAAACAATATTTATACAAAATATTTTTTAAAAAATGCATACTTGAAATTTTTTAATTAAAACATATTTCAAACCGACACTAAGTTGGCAGTAAATTTAATCATTTATTCGTTTTTTTTTCTTTCTCTAACAGGTGTTCTTGGTCGGTGACAGTGCCGGTGCAAACATCTCACACCACATGGCCCTACGAGCCAAAAACTCCGATCTCGCCGGAAAAATAAAAATTCTCGGAATTGCTCTAATCCAACCGTACTTCTGGGGGCAGGAACCCATCGGATCGGAGATCACTGAGCAGGAGCAAAAAGCTCAGGTGGATCGTTGGTGGAATTTCGTGTGCCCATCGGACAGAGGAAACGACGATCTGTTAATCAACCCGTTCGCCGACGGATCTCCGGCGATCGAGGGCCTCGCGGGGGAGAAGGCTGTGGTATTAGTTGCAGAGAAAGATATTCTGAGGGACAGAGGGAAGCTTTACTATGAGAATCTGGAGACGAGCGAGTGGAAAGGGAAGGTCGAGATTTTTGAGACGGAAGGGGAGGATCATGCTTTCCATATCCTCGATCCAACTTCTGAGAAAGCCAAGAGCCTTACAAAACGTTTGGCTTTGTTTATAAATGAAGATTGAACGTTGAAATTGAAACTGAGCTGCGACAGGGTCGCTTTCATGGCAGATTTACTCTAAGAATGTGCGAGTACGATGGAAAAATGACCTGTGAAACTTGGTTGTATAGTGTTTAGCTATCTCGAAACCCTAATGATACATGTCAATTAATGCTGAATTATATTTGCTTTGACTAGAAAATGATGATATTAAAAATATCTCCTTTGTGTAAGTTAAATTACGTATCTGATTGCCAACAAGAATTTAGCTTAACTAGCATAAAGATGTACTAATAACCACAAAGTTTAT

mRNA sequence

ATGGCTGCAGCAGATTCCAACGACCCAGAGCTGGATGTCGAGCTTCTTCCTTACCTTAAACTCTACAAAAACGGTGTCGTCGAGAGGCTTATCGGGACACGTGTCACTCCGCCGGGGCTCGATTCTCAAACTGGAGTTGACTCTAAAGATATCGTTATTGTCCCAGAAACTGGCGTCTCGGCTCGACTTTACCGTCCCACCGCCGTCGAACCTCGCCGGAAACTTCCCTTGGTTGTTTACTTTCACGGCGGCGCGTTTGTTATAGCCTCTAGTGCTGAACCTATCTACCATAACAATTGTCTCATCCCGCTCGCTGCTGAGGTTGAAAGCGTCGTCGTTTCGGTGAATTACAGATTAGCGCCGGAGCACCCTCTCCCCGCCGCTTACGACGATTCGTGGGCTGCGCTGCAGTGGATTGCCGCCCAGTCCCAGTCCTCCGGCGACGATGCCGACCATGAGCCTTGGCTTAAACAGTTGGTCGACTTCGAGAAGGTGTTCTTGGTCGGTGACAGTGCCGGTGCAAACATCTCACACCACATGGCCCTACGAGCCAAAAACTCCGATCTCGCCGGAAAAATAAAAATTCTCGGAATTGCTCTAATCCAACCGTACTTCTGGGGGCAGGAACCCATCGGATCGGAGATCACTGAGCAGGAGCAAAAAGCTCAGGTGGATCGTTGGTGGAATTTCGTGTGCCCATCGGACAGAGGAAACGACGATCTGTTAATCAACCCGTTCGCCGACGGATCTCCGGCGATCGAGGGCCTCGCGGGGGAGAAGGCTGTGGTATTAGTTGCAGAGAAAGATATTCTGAGGGACAGAGGGAAGCTTTACTATGAGAATCTGGAGACGAGCGAGTGGAAAGGGAAGGTCGAGATTTTTGAGACGGAAGGGGAGGATCATGCTTTCCATATCCTCGATCCAACTTCTGAGAAAGCCAAGAGCCTTACAAAACGTTTGGCTTTGTTTATAAATGAAGATTGA

Coding sequence (CDS)

ATGGCTGCAGCAGATTCCAACGACCCAGAGCTGGATGTCGAGCTTCTTCCTTACCTTAAACTCTACAAAAACGGTGTCGTCGAGAGGCTTATCGGGACACGTGTCACTCCGCCGGGGCTCGATTCTCAAACTGGAGTTGACTCTAAAGATATCGTTATTGTCCCAGAAACTGGCGTCTCGGCTCGACTTTACCGTCCCACCGCCGTCGAACCTCGCCGGAAACTTCCCTTGGTTGTTTACTTTCACGGCGGCGCGTTTGTTATAGCCTCTAGTGCTGAACCTATCTACCATAACAATTGTCTCATCCCGCTCGCTGCTGAGGTTGAAAGCGTCGTCGTTTCGGTGAATTACAGATTAGCGCCGGAGCACCCTCTCCCCGCCGCTTACGACGATTCGTGGGCTGCGCTGCAGTGGATTGCCGCCCAGTCCCAGTCCTCCGGCGACGATGCCGACCATGAGCCTTGGCTTAAACAGTTGGTCGACTTCGAGAAGGTGTTCTTGGTCGGTGACAGTGCCGGTGCAAACATCTCACACCACATGGCCCTACGAGCCAAAAACTCCGATCTCGCCGGAAAAATAAAAATTCTCGGAATTGCTCTAATCCAACCGTACTTCTGGGGGCAGGAACCCATCGGATCGGAGATCACTGAGCAGGAGCAAAAAGCTCAGGTGGATCGTTGGTGGAATTTCGTGTGCCCATCGGACAGAGGAAACGACGATCTGTTAATCAACCCGTTCGCCGACGGATCTCCGGCGATCGAGGGCCTCGCGGGGGAGAAGGCTGTGGTATTAGTTGCAGAGAAAGATATTCTGAGGGACAGAGGGAAGCTTTACTATGAGAATCTGGAGACGAGCGAGTGGAAAGGGAAGGTCGAGATTTTTGAGACGGAAGGGGAGGATCATGCTTTCCATATCCTCGATCCAACTTCTGAGAAAGCCAAGAGCCTTACAAAACGTTTGGCTTTGTTTATAAATGAAGATTGA

Protein sequence

MAAADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHAFHILDPTSEKAKSLTKRLALFINED
Homology
BLAST of Spg016746 vs. NCBI nr
Match: XP_038898530.1 (probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 594.7 bits (1532), Expect = 4.7e-166
Identity = 276/325 (84.92%), Postives = 309/325 (95.08%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           AADSN+PELD+ELLPYL+LY+NGVVERL+GTR+TPPGLD++TGVDSKDI+IVPETGVSAR
Sbjct: 2   AADSNNPELDLELLPYLRLYRNGVVERLLGTRITPPGLDTRTGVDSKDIIIVPETGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPTAV+P RKLPLVVYFHGGAF++ASSAEPIYHNNCLIPLAAE ++V++SVNYRLAPE
Sbjct: 62  LYRPTAVDPHRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAQTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMAL 182
           HPLPAAYDDSWAALQWIAAQSQ+S D+  HEPWLK+LVDFEKVFLVGDSAG NISHHMAL
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSQTSADEPGHEPWLKELVDFEKVFLVGDSAGGNISHHMAL 181

Query: 183 RAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLL 242
           RAK+S+L  KIK++GIALIQPYFWGQEPIGSEITE  ++A+VDRWWNFVCPSDRGNDDLL
Sbjct: 182 RAKSSNLGAKIKVVGIALIQPYFWGQEPIGSEITEHHKRAEVDRWWNFVCPSDRGNDDLL 241

Query: 243 INPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHA 302
           INPF+DGSPAI+GL GEK VV+VAEKDILRDRGKLYYENL  SEWKGKVEI+ETEGEDHA
Sbjct: 242 INPFSDGSPAIDGLTGEKVVVIVAEKDILRDRGKLYYENLAKSEWKGKVEIYETEGEDHA 301

Query: 303 FHILDPTSEKAKSLTKRLALFINED 328
           FH+LDPTSEKAK+L KRLA F+NED
Sbjct: 302 FHMLDPTSEKAKALIKRLAFFLNED 326

BLAST of Spg016746 vs. NCBI nr
Match: XP_023525571.1 (probable carboxylesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 576.6 bits (1485), Expect = 1.3e-160
Identity = 273/329 (82.98%), Postives = 301/329 (91.49%), Query Frame = 0

Query: 1   MAAADSNDP--ELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETG 60
           M A DSN+P  EL++ELLPYL++YKNGVVERL+GTRVTPPGLDS TGVDSKDI IVPETG
Sbjct: 1   MVAPDSNNPELELELELLPYLRVYKNGVVERLVGTRVTPPGLDSLTGVDSKDITIVPETG 60

Query: 61  VSARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYR 120
           ++ARLYRPTAVEP RKLPLVVYFHGGAF+I SSAEPIYHNNCLIPLAA  ESVV+SVNYR
Sbjct: 61  ITARLYRPTAVEPPRKLPLVVYFHGGAFLITSSAEPIYHNNCLIPLAAATESVVLSVNYR 120

Query: 121 LAPEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISH 180
           LAPEHPLPAAY+DSWAALQW+A QS++SGD+A  EPWLKQLVDFEKVFL+GDSAG NI+H
Sbjct: 121 LAPEHPLPAAYEDSWAALQWVAEQSKTSGDEAGQEPWLKQLVDFEKVFLLGDSAGGNIAH 180

Query: 181 HMALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGN 240
           HMALRA+NS+LAGK KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSDRGN
Sbjct: 181 HMALRAQNSNLAGKFKIRGIALIQPYFWGEEPIGSEITEPEKKALVDSWWNFVCPSDRGN 240

Query: 241 DDLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEG 300
           DDLLINPFADGSP++EGL GEK VV+VAEKDILRDRG +YYENL  S WKGKV+IFETEG
Sbjct: 241 DDLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMIYYENLAKSNWKGKVDIFETEG 300

Query: 301 EDHAFHILDPTSEKAKSLTKRLALFINED 328
           EDHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 301 EDHAFHLLDPTSEKAKRLVNRLALFVNED 329

BLAST of Spg016746 vs. NCBI nr
Match: XP_022982269.1 (probable carboxylesterase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 575.9 bits (1483), Expect = 2.3e-160
Identity = 274/329 (83.28%), Postives = 299/329 (90.88%), Query Frame = 0

Query: 1   MAAADSNDP--ELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETG 60
           M A DSN+P  EL++ELLPYL++YKNGVVERL+GTRVTPPGLDS TGVDSKDI IVPETG
Sbjct: 1   MVAPDSNNPELELELELLPYLRVYKNGVVERLVGTRVTPPGLDSLTGVDSKDITIVPETG 60

Query: 61  VSARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYR 120
           +SARLYRPTAVEP RKLPL+VYFHGGAF+I SSAEPIYHNNCLIPLAA  ESVV+SVNYR
Sbjct: 61  ISARLYRPTAVEPPRKLPLIVYFHGGAFLITSSAEPIYHNNCLIPLAAATESVVLSVNYR 120

Query: 121 LAPEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISH 180
           LAPEHPLPAAYDDSWAALQW+A QS++SGD+A  EPWLKQLVDF+KVFL GDSAG NI+H
Sbjct: 121 LAPEHPLPAAYDDSWAALQWVAEQSKTSGDEAGQEPWLKQLVDFKKVFLFGDSAGGNIAH 180

Query: 181 HMALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGN 240
           HMALRA+NS LAGK KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSDRGN
Sbjct: 181 HMALRAQNSHLAGKFKIRGIALIQPYFWGREPIGSEITEPEKKALVDGWWNFVCPSDRGN 240

Query: 241 DDLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEG 300
           DDLLINPFADGSP++EGL GEK VV+VAEKDILRDRG LYYENL  S WKGKV+IFETEG
Sbjct: 241 DDLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLAKSHWKGKVDIFETEG 300

Query: 301 EDHAFHILDPTSEKAKSLTKRLALFINED 328
           EDHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 301 EDHAFHLLDPTSEKAKCLVNRLALFVNED 329

BLAST of Spg016746 vs. NCBI nr
Match: XP_011654101.1 (probable carboxylesterase 2 [Cucumis sativus] >KGN55177.1 hypothetical protein Csa_012076 [Cucumis sativus])

HSP 1 Score: 575.9 bits (1483), Expect = 2.3e-160
Identity = 269/325 (82.77%), Postives = 302/325 (92.92%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A D N+PELDVELLPYL+LYKNGVVERL+GTRVTPPGLDS+TGV SKDIVIVP+TGVSAR
Sbjct: 2   ATDLNNPELDVELLPYLRLYKNGVVERLLGTRVTPPGLDSRTGVHSKDIVIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPTAV+P RKLPLVVYFHGGAF++ASSAEP+YHNNCLIPLAAE ++V++SVNYRLAPE
Sbjct: 62  LYRPTAVDPGRKLPLVVYFHGGAFLVASSAEPVYHNNCLIPLAAEAQTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMAL 182
           HPLPAAYDDSWAALQWIAAQS+SS D+  HEPWLK+LVDFEKVFLVGDSAG NI HHMAL
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSKSSADEPGHEPWLKELVDFEKVFLVGDSAGGNICHHMAL 181

Query: 183 RAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLL 242
           RAKNS+L  KIKI+GIALIQPYFWGQEPIGSEITE  +KA+VD WWNFVCPSDRGNDDLL
Sbjct: 182 RAKNSNLGAKIKIVGIALIQPYFWGQEPIGSEITEHHKKAEVDSWWNFVCPSDRGNDDLL 241

Query: 243 INPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHA 302
           INPF+DGSPAI+GLAGE+ +V+VA KDILR+RGKLYYE L  SEWKGKVE +ETEGEDHA
Sbjct: 242 INPFSDGSPAIDGLAGERVLVIVAGKDILRERGKLYYETLANSEWKGKVEFYETEGEDHA 301

Query: 303 FHILDPTSEKAKSLTKRLALFINED 328
           FH+L+P+SEKAK+L KRLA F+N+D
Sbjct: 302 FHMLNPSSEKAKALLKRLAFFLNQD 326

BLAST of Spg016746 vs. NCBI nr
Match: XP_008452527.1 (PREDICTED: probable carboxylesterase 2 [Cucumis melo] >KAA0064370.1 putative carboxylesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 575.5 bits (1482), Expect = 3.0e-160
Identity = 269/325 (82.77%), Postives = 300/325 (92.31%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A DSN+PEL VELLPYL+LYKNG+V+RLIGTRVTPPGLDS+TGVDSKDI+IVP+TGVSAR
Sbjct: 2   ATDSNNPELAVELLPYLRLYKNGIVDRLIGTRVTPPGLDSRTGVDSKDIIIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPT V+PRRKLPLVVYFHGGAF++ASSAEPIYHNNCLIPLAAE  +V++SVNYRLAPE
Sbjct: 62  LYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMAL 182
           HPLPAAYDDSWAALQWIAAQSQSS D   HEPWL++LVDFEKVFLVGDSAG NISHHMAL
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMAL 181

Query: 183 RAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLL 242
           RAKNS+L  KIKI+GIALIQPYFWGQEPIG EI E  ++A+VD WWNFVCPSDRGNDDLL
Sbjct: 182 RAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDLL 241

Query: 243 INPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHA 302
           INPF+DGSPAI+GLAGEK +V+VA KDILR+RGKLYYE L  SEWKGKVE +ETEGEDHA
Sbjct: 242 INPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDHA 301

Query: 303 FHILDPTSEKAKSLTKRLALFINED 328
           FH+L+PTSEKAK+L KRLA F+N+D
Sbjct: 302 FHMLNPTSEKAKALVKRLAFFLNQD 326

BLAST of Spg016746 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.1e-104
Identity = 179/322 (55.59%), Postives = 236/322 (73.29%), Query Frame = 0

Query: 5   DSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLY 64
           +S   ++ +ELLP+L ++ +G VERL GT V PPGLD  TGV SKDI+I P+TG+SAR+Y
Sbjct: 2   ESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIY 61

Query: 65  RPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHP 124
           RP +++P +K+PL++YFHGGAF+I+S++ P YH + L  +  +   + VSVNYRLAPEHP
Sbjct: 62  RPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTS-LNKIVNQANVIAVSVNYRLAPEHP 121

Query: 125 LPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRA 184
           LP AY+DSW AL+ I          A +EPW+    D + +FLVGDSAGANISHH+A RA
Sbjct: 122 LPTAYEDSWTALKNI---------QAINEPWINDYADLDSLFLVGDSAGANISHHLAFRA 181

Query: 185 KNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLLIN 244
           K SD   KIK  GI +I PYFWG +PIG+EI ++ +K  VD WW FVCPS++G+DD  IN
Sbjct: 182 KQSDQTLKIK--GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWIN 241

Query: 245 PFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHAFH 304
           PFADGSP + GL  E+ ++ VAEKDIL +RGK+YYE L  SEWKGKVEI ET+ +DH FH
Sbjct: 242 PFADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFH 301

Query: 305 ILDPTSEKAKSLTKRLALFINE 327
           I +P  ++A  + + LALFIN+
Sbjct: 302 IFEPDCDEAMEMVRCLALFINQ 311

BLAST of Spg016746 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 9.8e-74
Identity = 142/325 (43.69%), Postives = 201/325 (61.85%), Query Frame = 0

Query: 2   AAADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPG-LDSQTGVDSKDIVIVPETGVS 61
           + + +   E+D EL P L++YK+G VER +G+   PP   D +TGV +KDIVI     +S
Sbjct: 4   STSTTTSKEIDRELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTIS 63

Query: 62  ARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLA 121
           AR+Y P       KLP++VY+HGGAF +  SA    H   L  +A++   +VVS+ YRLA
Sbjct: 64  ARVYLPKLNNTTEKLPILVYYHGGAFCL-ESAFSFLHQRYLNIVASKANVLVVSIEYRLA 123

Query: 122 PEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHM 181
           PEHPLPAAY+D W AL+W+ + S ++    + +PWL +  DF + ++ GD++GANI+H+ 
Sbjct: 124 PEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNA 183

Query: 182 ALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGN-D 241
           ALR     L G ++I G+    P FWG +P+ SE  E  +K+   + WNFV P   G  D
Sbjct: 184 ALRVGAEALPGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGID 243

Query: 242 DLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGE 301
           + LINP A G+P +  L   K +V VA KD LRDRG  YYE ++ S WKG VE+ + EGE
Sbjct: 244 NPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGE 303

Query: 302 DHAFHILDPTSEKAKSLTKRLALFI 325
           +H F I  P +E +K L  R+A F+
Sbjct: 304 EHCFQIYHPETENSKDLIGRIASFL 327

BLAST of Spg016746 vs. ExPASy Swiss-Prot
Match: Q5NUF3 (2-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.4e-72
Identity = 137/317 (43.22%), Postives = 200/317 (63.09%), Query Frame = 0

Query: 10  ELDVELLPYLKLYKNGVVERLIGT-RVTPPGLDSQTGVDSKDIVIVPETGVSARLYRPTA 69
           E+  ELLP +++YK+G VERL+ +  V     D QTGV SKDIVI     VSAR++ P +
Sbjct: 4   EIVKELLPLIRVYKDGSVERLLSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPKS 63

Query: 70  VEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLPAA 129
                KLP+ +YFHGGAF + S+     H    I LA+E   + +SV++RL P HP+PAA
Sbjct: 64  HHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNI-LASEANIIAISVDFRLLPHHPIPAA 123

Query: 130 YDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRAKNSD 189
           Y+D W  L+WIA+ + ++ +  + EPWL    DF KV++ G+++GANI+H++ LRA N  
Sbjct: 124 YEDGWTTLKWIASHANNT-NTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNES 183

Query: 190 LAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGN-DDLLINPFA 249
           L G +KILG  L  P+FWG +PIGSE  E  +++   + WNF CP   G  D+  INP  
Sbjct: 184 LPGDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCV 243

Query: 250 DGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHAFHILD 309
            G+P++  LA  K +V +  KD  RDR  LY+  +E S W+G++++F+   E+HAF +  
Sbjct: 244 PGAPSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFK 303

Query: 310 PTSEKAKSLTKRLALFI 325
           P +  AK++ KRLA F+
Sbjct: 304 PETHLAKAMIKRLASFL 318

BLAST of Spg016746 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.2e-72
Identity = 141/318 (44.34%), Postives = 201/318 (63.21%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRPT 67
           D E+ ++L P+L +YK+G +ER +GT V P    +   V +KD+VI P TGVS RLY P 
Sbjct: 80  DDEIVLDLKPFLIIYKSGRIERFLGTTVIP----ACPEVATKDVVIDPATGVSVRLYLPN 139

Query: 68  AVE-PRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLP 127
            V+ P +KLP++VYFHGG FVI ++  P YH N L  LAA+   ++VS+NYRLAPE+PLP
Sbjct: 140 VVDLPSKKLPVLVYFHGGGFVIENTGSPNYH-NYLTLLAAKAGVLIVSINYRLAPEYPLP 199

Query: 128 AAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRAKN 187
           A+YDD  A   W+ + S         EPWL Q  DF ++ L GDSAG N++H++A+RA  
Sbjct: 200 ASYDDCMAGFNWVVSHSAGPA----LEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRAD- 259

Query: 188 SDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLLINPF 247
              AG I+  G+A++ PYF G EP+G+EI +       D+ W    P   G DD LINP 
Sbjct: 260 ---AGVIE--GVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPV 319

Query: 248 ADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHAFHIL 307
           A G+P++ GL  ++AVV VA  D L +RG++YYE L  S W+G+ E+ + EG  H FH+ 
Sbjct: 320 APGAPSLAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHVFHLS 379

Query: 308 DPTSEKAKSLTKRLALFI 325
           D + + + ++  +L  F+
Sbjct: 380 DYSGDISVAMMTKLIAFL 382

BLAST of Spg016746 vs. ExPASy Swiss-Prot
Match: I4DST9 (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 141/318 (44.34%), Postives = 199/318 (62.58%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRPT 67
           D E+ ++L P+L +YK+G +ER +GT V P    +   V +KD+VI P TGVS RLY P 
Sbjct: 77  DDEIVLDLKPFLIIYKSGRIERFLGTTVIP----ACPEVATKDVVIDPATGVSVRLYLPN 136

Query: 68  AVE-PRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLP 127
            V+ P +KLP++VYFHGG FVI ++  P YH N L  LAA+   ++VS+NYRLAPE+PLP
Sbjct: 137 VVDLPSKKLPVLVYFHGGGFVIENTGSPNYH-NYLTLLAAKAGVLIVSINYRLAPEYPLP 196

Query: 128 AAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRAKN 187
           A+YDD  A   W+ + S         EPWL Q  DF ++ L GDSAG N++H++A+RA  
Sbjct: 197 ASYDDCMAGFNWVVSHSAGPA----LEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRAD- 256

Query: 188 SDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLLINPF 247
              AG I+  G+A++ PYF G EP+G+EI +       D+ W    P   G DD LINP 
Sbjct: 257 ---AGVIE--GVAIVHPYFLGSEPVGNEINDPANIEFHDKLWRLAAPDTEGLDDPLINPV 316

Query: 248 ADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHAFHIL 307
           A G+P + GL  ++AVV VA  D L +RG++YYE L  S W G+ E+ + EG  H FH+ 
Sbjct: 317 APGAPILAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWGGEAELVQHEGVGHVFHLS 376

Query: 308 DPTSEKAKSLTKRLALFI 325
           D + + + ++  +L  F+
Sbjct: 377 DYSGDISVAMMTKLIAFL 379

BLAST of Spg016746 vs. ExPASy TrEMBL
Match: A0A6J1J426 (probable carboxylesterase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481148 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.1e-160
Identity = 274/329 (83.28%), Postives = 299/329 (90.88%), Query Frame = 0

Query: 1   MAAADSNDP--ELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETG 60
           M A DSN+P  EL++ELLPYL++YKNGVVERL+GTRVTPPGLDS TGVDSKDI IVPETG
Sbjct: 1   MVAPDSNNPELELELELLPYLRVYKNGVVERLVGTRVTPPGLDSLTGVDSKDITIVPETG 60

Query: 61  VSARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYR 120
           +SARLYRPTAVEP RKLPL+VYFHGGAF+I SSAEPIYHNNCLIPLAA  ESVV+SVNYR
Sbjct: 61  ISARLYRPTAVEPPRKLPLIVYFHGGAFLITSSAEPIYHNNCLIPLAAATESVVLSVNYR 120

Query: 121 LAPEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISH 180
           LAPEHPLPAAYDDSWAALQW+A QS++SGD+A  EPWLKQLVDF+KVFL GDSAG NI+H
Sbjct: 121 LAPEHPLPAAYDDSWAALQWVAEQSKTSGDEAGQEPWLKQLVDFKKVFLFGDSAGGNIAH 180

Query: 181 HMALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGN 240
           HMALRA+NS LAGK KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSDRGN
Sbjct: 181 HMALRAQNSHLAGKFKIRGIALIQPYFWGREPIGSEITEPEKKALVDGWWNFVCPSDRGN 240

Query: 241 DDLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEG 300
           DDLLINPFADGSP++EGL GEK VV+VAEKDILRDRG LYYENL  S WKGKV+IFETEG
Sbjct: 241 DDLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLAKSHWKGKVDIFETEG 300

Query: 301 EDHAFHILDPTSEKAKSLTKRLALFINED 328
           EDHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 301 EDHAFHLLDPTSEKAKCLVNRLALFVNED 329

BLAST of Spg016746 vs. ExPASy TrEMBL
Match: A0A0A0L3I9 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639160 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.1e-160
Identity = 269/325 (82.77%), Postives = 302/325 (92.92%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A D N+PELDVELLPYL+LYKNGVVERL+GTRVTPPGLDS+TGV SKDIVIVP+TGVSAR
Sbjct: 2   ATDLNNPELDVELLPYLRLYKNGVVERLLGTRVTPPGLDSRTGVHSKDIVIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPTAV+P RKLPLVVYFHGGAF++ASSAEP+YHNNCLIPLAAE ++V++SVNYRLAPE
Sbjct: 62  LYRPTAVDPGRKLPLVVYFHGGAFLVASSAEPVYHNNCLIPLAAEAQTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMAL 182
           HPLPAAYDDSWAALQWIAAQS+SS D+  HEPWLK+LVDFEKVFLVGDSAG NI HHMAL
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSKSSADEPGHEPWLKELVDFEKVFLVGDSAGGNICHHMAL 181

Query: 183 RAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLL 242
           RAKNS+L  KIKI+GIALIQPYFWGQEPIGSEITE  +KA+VD WWNFVCPSDRGNDDLL
Sbjct: 182 RAKNSNLGAKIKIVGIALIQPYFWGQEPIGSEITEHHKKAEVDSWWNFVCPSDRGNDDLL 241

Query: 243 INPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHA 302
           INPF+DGSPAI+GLAGE+ +V+VA KDILR+RGKLYYE L  SEWKGKVE +ETEGEDHA
Sbjct: 242 INPFSDGSPAIDGLAGERVLVIVAGKDILRERGKLYYETLANSEWKGKVEFYETEGEDHA 301

Query: 303 FHILDPTSEKAKSLTKRLALFINED 328
           FH+L+P+SEKAK+L KRLA F+N+D
Sbjct: 302 FHMLNPSSEKAKALLKRLAFFLNQD 326

BLAST of Spg016746 vs. ExPASy TrEMBL
Match: A0A5A7VAW2 (Putative carboxylesterase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00710 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 269/325 (82.77%), Postives = 300/325 (92.31%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A DSN+PEL VELLPYL+LYKNG+V+RLIGTRVTPPGLDS+TGVDSKDI+IVP+TGVSAR
Sbjct: 2   ATDSNNPELAVELLPYLRLYKNGIVDRLIGTRVTPPGLDSRTGVDSKDIIIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPT V+PRRKLPLVVYFHGGAF++ASSAEPIYHNNCLIPLAAE  +V++SVNYRLAPE
Sbjct: 62  LYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMAL 182
           HPLPAAYDDSWAALQWIAAQSQSS D   HEPWL++LVDFEKVFLVGDSAG NISHHMAL
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMAL 181

Query: 183 RAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLL 242
           RAKNS+L  KIKI+GIALIQPYFWGQEPIG EI E  ++A+VD WWNFVCPSDRGNDDLL
Sbjct: 182 RAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDLL 241

Query: 243 INPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHA 302
           INPF+DGSPAI+GLAGEK +V+VA KDILR+RGKLYYE L  SEWKGKVE +ETEGEDHA
Sbjct: 242 INPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDHA 301

Query: 303 FHILDPTSEKAKSLTKRLALFINED 328
           FH+L+PTSEKAK+L KRLA F+N+D
Sbjct: 302 FHMLNPTSEKAKALVKRLAFFLNQD 326

BLAST of Spg016746 vs. ExPASy TrEMBL
Match: A0A1S3BU25 (probable carboxylesterase 2 OS=Cucumis melo OX=3656 GN=LOC103493527 PE=4 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.4e-160
Identity = 269/325 (82.77%), Postives = 300/325 (92.31%), Query Frame = 0

Query: 3   AADSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSAR 62
           A DSN+PEL VELLPYL+LYKNG+V+RLIGTRVTPPGLDS+TGVDSKDI+IVP+TGVSAR
Sbjct: 2   ATDSNNPELAVELLPYLRLYKNGIVDRLIGTRVTPPGLDSRTGVDSKDIIIVPDTGVSAR 61

Query: 63  LYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 122
           LYRPT V+PRRKLPLVVYFHGGAF++ASSAEPIYHNNCLIPLAAE  +V++SVNYRLAPE
Sbjct: 62  LYRPTVVDPRRKLPLVVYFHGGAFLVASSAEPIYHNNCLIPLAAEAHTVLLSVNYRLAPE 121

Query: 123 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMAL 182
           HPLPAAYDDSWAALQWIAAQSQSS D   HEPWL++LVDFEKVFLVGDSAG NISHHMAL
Sbjct: 122 HPLPAAYDDSWAALQWIAAQSQSSADQPGHEPWLEELVDFEKVFLVGDSAGGNISHHMAL 181

Query: 183 RAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLL 242
           RAKNS+L  KIKI+GIALIQPYFWGQEPIG EI E  ++A+VD WWNFVCPSDRGNDDLL
Sbjct: 182 RAKNSNLGAKIKIVGIALIQPYFWGQEPIGLEIIEHHKRAEVDSWWNFVCPSDRGNDDLL 241

Query: 243 INPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHA 302
           INPF+DGSPAI+GLAGEK +V+VA KDILR+RGKLYYE L  SEWKGKVE +ETEGEDHA
Sbjct: 242 INPFSDGSPAIDGLAGEKVLVIVAGKDILRERGKLYYETLARSEWKGKVEFYETEGEDHA 301

Query: 303 FHILDPTSEKAKSLTKRLALFINED 328
           FH+L+PTSEKAK+L KRLA F+N+D
Sbjct: 302 FHMLNPTSEKAKALVKRLAFFLNQD 326

BLAST of Spg016746 vs. ExPASy TrEMBL
Match: A0A6J1FKX0 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446350 PE=4 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.9e-160
Identity = 273/329 (82.98%), Postives = 300/329 (91.19%), Query Frame = 0

Query: 1   MAAADSNDP--ELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETG 60
           M A DSN+P  EL++ELLPYL++YKNGVVERL+GTRVTPPGLDS TGVDSKDI IVPETG
Sbjct: 1   MVAPDSNNPELELELELLPYLRVYKNGVVERLVGTRVTPPGLDSLTGVDSKDITIVPETG 60

Query: 61  VSARLYRPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYR 120
           ++ARLYRPTAVEP RKLPLVVYFHGGAF+I SSAEPIYHNNCLIPLAA  ESVV+SVNYR
Sbjct: 61  ITARLYRPTAVEPPRKLPLVVYFHGGAFLITSSAEPIYHNNCLIPLAAATESVVLSVNYR 120

Query: 121 LAPEHPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISH 180
           LAPEHPLPAAY+DSWAALQW+A QS++SGD+A  EPWLKQLVDFEKVFL+GDSAG NI+H
Sbjct: 121 LAPEHPLPAAYEDSWAALQWVAEQSKTSGDEAGQEPWLKQLVDFEKVFLLGDSAGGNIAH 180

Query: 181 HMALRAKNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGN 240
           HMALRA+NS+LAG  KI GIALIQPYFWG+EPIGSEITE E+KA VD WWNFVCPSDRGN
Sbjct: 181 HMALRAQNSNLAGNFKIRGIALIQPYFWGEEPIGSEITEPEKKALVDSWWNFVCPSDRGN 240

Query: 241 DDLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEG 300
           DDLLINPFADGSP++EGL GEK VV+VAEKDILRDRG LYYENL  S WKGKV+IFETEG
Sbjct: 241 DDLLINPFADGSPSVEGLVGEKVVVIVAEKDILRDRGMLYYENLVKSHWKGKVDIFETEG 300

Query: 301 EDHAFHILDPTSEKAKSLTKRLALFINED 328
           EDHAFH+LDPTSEKAK L  RLALF+NED
Sbjct: 301 EDHAFHLLDPTSEKAKRLVNRLALFVNED 329

BLAST of Spg016746 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 6.5e-105
Identity = 179/322 (55.59%), Postives = 236/322 (73.29%), Query Frame = 0

Query: 5   DSNDPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLY 64
           +S   ++ +ELLP+L ++ +G VERL GT V PPGLD  TGV SKDI+I P+TG+SAR+Y
Sbjct: 2   ESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIY 61

Query: 65  RPTAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHP 124
           RP +++P +K+PL++YFHGGAF+I+S++ P YH + L  +  +   + VSVNYRLAPEHP
Sbjct: 62  RPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTS-LNKIVNQANVIAVSVNYRLAPEHP 121

Query: 125 LPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRA 184
           LP AY+DSW AL+ I          A +EPW+    D + +FLVGDSAGANISHH+A RA
Sbjct: 122 LPTAYEDSWTALKNI---------QAINEPWINDYADLDSLFLVGDSAGANISHHLAFRA 181

Query: 185 KNSDLAGKIKILGIALIQPYFWGQEPIGSEITEQEQKAQVDRWWNFVCPSDRGNDDLLIN 244
           K SD   KIK  GI +I PYFWG +PIG+EI ++ +K  VD WW FVCPS++G+DD  IN
Sbjct: 182 KQSDQTLKIK--GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWIN 241

Query: 245 PFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHAFH 304
           PFADGSP + GL  E+ ++ VAEKDIL +RGK+YYE L  SEWKGKVEI ET+ +DH FH
Sbjct: 242 PFADGSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFH 301

Query: 305 ILDPTSEKAKSLTKRLALFINE 327
           I +P  ++A  + + LALFIN+
Sbjct: 302 IFEPDCDEAMEMVRCLALFINQ 311

BLAST of Spg016746 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 265.0 bits (676), Expect = 8.0e-71
Identity = 141/332 (42.47%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRP- 67
           D E+  +  P L +YK+G +ERL+G    PP  + Q GV SKD+V  P+  +S R+Y P 
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPE 61

Query: 68  ----TAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPE 127
                  E   KLPL+VYFHGG F++ ++  P YH      ++A  + V VSV+YR APE
Sbjct: 62  KAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSAS-DCVAVSVDYRRAPE 121

Query: 128 HPLPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMAL 187
           HP+P +YDDSW AL+W+ +    SG     E WL +  DF KVFL GDSAGANI+HHM +
Sbjct: 122 HPIPTSYDDSWTALKWVFSHIAGSGS----EDWLNKHADFSKVFLAGDSAGANITHHMTM 181

Query: 188 RAKNSDLA----GKIKILGIALIQPYFWGQEPI-GSEITEQEQKAQVDRWWNFVCPSDR- 247
           +A    L+     +  I GI L+ PYFW + P+   E T+   +  ++  W    P+ + 
Sbjct: 182 KAAKDKLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKD 241

Query: 248 GNDDLLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKV-EIFE 307
           G+DD  IN     S  + GL   K +V+VAEKD L  +G  Y+E L  S W G+V ++ E
Sbjct: 242 GSDDPFINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVE 301

Query: 308 TEGEDHAFHILDPTSEKAKSLTKRLALFINED 328
           T+GE H FH+ DP SEKA  L  R A FI  D
Sbjct: 302 TKGEGHVFHLRDPNSEKAHELVHRFAGFIKGD 328

BLAST of Spg016746 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.0e-70
Identity = 140/324 (43.21%), Postives = 191/324 (58.95%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRP- 67
           D E+ V+  P LK+YK+G +ERL+G    PP  + Q GV SKD+V   +  +S R+Y P 
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPE 61

Query: 68  -TAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPL 127
             A E   KLPL+VYFHGG F+I ++  P YH      ++A    V VSV+YR APEHP+
Sbjct: 62  KAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSAS-NCVAVSVDYRRAPEHPI 121

Query: 128 PAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRAK 187
              +DDSW AL+W+      SG     E WL +  DF +VFL GDSAGANI HHMA+RA 
Sbjct: 122 SVPFDDSWTALKWVFTHITGSG----QEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAA 181

Query: 188 NSDLAGKIK---ILGIALIQPYFWGQEPIGSEITEQEQ-KAQVDRWWNFVCPSDR-GNDD 247
              L+  +    I GI L+ PYFW + PI  + T+ E  + +++ +W    P+ + G DD
Sbjct: 182 KEKLSPGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDD 241

Query: 248 LLINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGED 307
            L+N     S  + GL   K +V+VAEKD L  +G  Y   LE S WKG+VE+ E+EGED
Sbjct: 242 PLLNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGED 301

Query: 308 HAFHILDPTSEKAKSLTKRLALFI 325
           H FH+L P  + A  +  + + FI
Sbjct: 302 HVFHLLKPECDNAIEVMHKFSGFI 320

BLAST of Spg016746 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 258.1 bits (658), Expect = 9.7e-69
Identity = 139/323 (43.03%), Postives = 189/323 (58.51%), Query Frame = 0

Query: 8   DPELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRP- 67
           + E+  E LP+ ++YK+G VERLIGT   P  LD    V SKD++  PE  +S RL+ P 
Sbjct: 2   ESEIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPH 61

Query: 68  --TAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHP 127
             T +    KLPL++Y HGGA++I S   P+YH N L  +      + VSV YR APE P
Sbjct: 62  KSTKLTAGNKLPLLIYIHGGAWIIESPFSPLYH-NYLTEVVKSANCLAVSVQYRRAPEDP 121

Query: 128 LPAAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRA 187
           +PAAY+D W+A+QWI A S  SG       W+ +  DF KVFL GDSAG NISHHMA++A
Sbjct: 122 VPAAYEDVWSAIQWIFAHSNGSGP----VDWINKHADFGKVFLGGDSAGGNISHHMAMKA 181

Query: 188 KNSDLAGKIKILGIALIQPYFWGQEPIGS-EITEQEQKAQVDRWWNFVCP--SDRGNDDL 247
              +    +KI GIA++ P FWG +P+   ++ ++E ++ +   W  +    S  G DD 
Sbjct: 182 -GKEKKLDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDP 241

Query: 248 LINPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDH 307
           L N    GS    GL  +K +V VA KD+   +G  Y   LE  EW+G VE+ E EGEDH
Sbjct: 242 LFNVNGSGSD-FSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDH 301

Query: 308 AFHILDPTSEKAKSLTKRLALFI 325
            FH+ +P S+KA    K+   FI
Sbjct: 302 VFHLQNPKSDKALKFLKKFVEFI 317

BLAST of Spg016746 vs. TAIR 10
Match: AT1G49650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.5e-66
Identity = 137/321 (42.68%), Postives = 191/321 (59.50%), Query Frame = 0

Query: 10  ELDVELLPYLKLYKNGVVERLIGTRVTPPGLDSQTGVDSKDIVIVPETGVSARLYRP--- 69
           E+  E  P++++YK+G +ERL GT   P  L+ +  V SKD+V  P   +S RL+ P   
Sbjct: 60  EIISEHPPFVRVYKDGRIERLSGTETVPASLNPRNDVVSKDVVYSPGHNLSVRLFLPHKS 119

Query: 70  TAVEPRRKLPLVVYFHGGAFVIASSAEPIYHNNCLIPLAAEVESVVVSVNYRLAPEHPLP 129
           T +    KLPL++YFHGGA++  S   PIYH N L  +      + VSV YR APE P+P
Sbjct: 120 TQLAAGNKLPLLIYFHGGAWINESPFSPIYH-NFLTEVVKSANCLAVSVQYRRAPEDPVP 179

Query: 130 AAYDDSWAALQWIAAQSQSSGDDADHEPWLKQLVDFEKVFLVGDSAGANISHHMALRAKN 189
           AAY+D+W+A+QWI + S  SG+    E W+ +  DFE+VFL GDSAG NISHHMA+RA  
Sbjct: 180 AAYEDTWSAIQWIFSHSCGSGE----EDWINKYADFERVFLAGDSAGGNISHHMAMRAGK 239

Query: 190 SDLAGKIKILGIALIQPYFWGQEPIGS-EITEQEQKAQV-DRWWNFVCP-SDRGNDDLLI 249
             L  +IK  G  ++ P  WG++P+   ++ ++E +  V + W   V P S  G DD   
Sbjct: 240 EKLKPRIK--GTVIVHPAIWGKDPVDEHDVQDREIRDGVAEVWEKIVSPNSVDGADDPWF 299

Query: 250 NPFADGSPAIEGLAGEKAVVLVAEKDILRDRGKLYYENLETSEWKGKVEIFETEGEDHAF 309
           N    GS    G+  +K +V VA KD+   +G  Y   L+ S WKG+VE+ E E E+H F
Sbjct: 300 NVVGSGS-NFSGMGCDKVLVEVAGKDVFWRQGLAYAAKLKKSGWKGEVEVIEEEDEEHCF 359

Query: 310 HILDPTSEKAKSLTKRLALFI 325
           H+L+P+SE A S  KR   FI
Sbjct: 360 HLLNPSSENAPSFMKRFVEFI 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898530.14.7e-16684.92probable carboxylesterase 2 [Benincasa hispida][more]
XP_023525571.11.3e-16082.98probable carboxylesterase 2 [Cucurbita pepo subsp. pepo][more]
XP_022982269.12.3e-16083.28probable carboxylesterase 2 isoform X1 [Cucurbita maxima][more]
XP_011654101.12.3e-16082.77probable carboxylesterase 2 [Cucumis sativus] >KGN55177.1 hypothetical protein C... [more]
XP_008452527.13.0e-16082.77PREDICTED: probable carboxylesterase 2 [Cucumis melo] >KAA0064370.1 putative car... [more]
Match NameE-valueIdentityDescription
Q9SX789.1e-10455.59Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q5NUF49.8e-7443.692-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Q5NUF32.4e-7243.222-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1[more]
I4DST83.2e-7244.34Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
I4DST91.6e-7144.34Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1J4261.1e-16083.28probable carboxylesterase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114811... [more]
A0A0A0L3I91.1e-16082.77Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G6391... [more]
A0A5A7VAW21.4e-16082.77Putative carboxylesterase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3BU251.4e-16082.77probable carboxylesterase 2 OS=Cucumis melo OX=3656 GN=LOC103493527 PE=4 SV=1[more]
A0A6J1FKX01.9e-16082.98probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111446350 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G47480.16.5e-10555.59alpha/beta-Hydrolases superfamily protein [more]
AT3G48700.18.0e-7142.47carboxyesterase 13 [more]
AT3G48690.11.0e-7043.21alpha/beta-Hydrolases superfamily protein [more]
AT1G49660.19.7e-6943.03carboxyesterase 5 [more]
AT1G49650.13.5e-6642.68alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 78..304
e-value: 3.0E-51
score: 174.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 18..327
e-value: 2.1E-72
score: 246.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 22..326
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 9..325
NoneNo IPR availablePANTHERPTHR23024:SF549RECEPTOR GID1, PUTATIVE-RELATEDcoord: 9..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016746.1Spg016746.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity