Spg016624 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016624
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationscaffold9: 45691483 .. 45695421 (+)
RNA-Seq ExpressionSpg016624
SyntenySpg016624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGCAAATTAAAAATGGCTCTATAAATATGAAGCAGCAAAACATCTTTCTCACACACAAAATTCTCAACCTGTTTAGTTGGTGTCTATACGACATCAACAAAACACACCACATCTAAAACCAGCTGCCCACAATCCTTTTTTCCAATTTTTGTTGTCATAATTAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAATGGGTCCTGAAGATCACCAATTTAGTGATGATTATCTGATATTGAAGCCTGAGAGTGTAAGTTGGTTTGATCTGTTTCTCTTCCTTTTTCCTTTCGGTTTCGTAGACATCAGAGCGTTGGCTGACATCCCCGAAGGCAAAGAAGATTCTTACAATAGCTTTGGAGTCCGATGGGTTATCTTCGTCTCCGTTTTCTTGCAGAAACTCCTTGATGCCATCGCCAATCTCTTCAAACTATTCAAATTTTTCCGTCGTATTTTTACCAAGAGAGGTCAAATTTCATATGCTCCAACTTTTTGTTGAGAGAATGAAAATATTGTTAAATATAATTAATAGTGGGACGTGGGCATGCATGCATGCAGGAAAGTTCTCGGGGATGCCTGGTAAAACTTACGCAACGGAGGTGAAATGTAGAGATTGGAGAATAGAAGTGGCCGTGGGGAAGAGGAAGAACATAAAATACGGTGAGGAGGATTTCAGATTTGATGGTTCCCTAACCATGATGGCTTCCGCTTTAGCCTACGAAGACTATTCACCAGCCCCCTCCGTCGTCCAGACCGTCGTAAATAACTGCTGGAAGGTATATTATATATGCGCTGCTGCTTCTTATGACGAGCTTTGGAGTCTTATATTTAATTGTTCAAATCTCCTGCATTAACCAACTTTAATTTTATTTGAGGCTTCATTTTCGTATAAACTACTGAGTTCTATAGATTTTTTATTTTCTTTTTGAAATTATTGGGTGAATACAGTGAAACTTATACGTCAGTTATGAATCGAAACATTTATCTAAATTCAATTTGATGGATTGAAGTTTAAGGTGAAGTTTGTTGATTTCAAAGATAAAAATTGATTTTTTTCGGGTTTTTTTTTTTTTTGCAAAAATGATTATTATTATTATACAATATATCTGTTTAATTGTGTTTCCTTTTCGATGCAGATGAAGCTACTTGACTGCTACGATGATTTTTGGAATGGTAAGACTTTCAGGTTCTAATAAGATATGGGTCATATAATTAAAATAGATTATAAATATGGTTTTTTTTTTCTCTCTCTTTTGTTGAGAATATGACTCGGATTAGATTGGAGAGTTGAACTTATAACTTTTAGAAAAATCAGAAATGTTTTGACCCATTCTTATGTTAAAGCTACTAAAATTAACTACTATATGTTGAAATAATGATGATACATCAATTAATGTACATCAATTAATGTTGTAGTCATTAATTAACATAAAGACCCATTTACTATATGTGATATGATCATGATAAATCAATATATTTATGTCAATATAAACATAGTTCAACTAGAACATCGAACTTATATCTTCCATTCCAATATTTCTTAACTAAAAAATAAAATAAAAATATCTATATATTGATTAATAGTAATCACTTAATTACTTAGTGATTTATCATTATATACTAATAATAGTAACCTCCGATTTATTTATATCTTAATATTTTAATTTTGCTATATTGTAATTATTTATTATAATAATTAATTTGCAACCGAAAACTACCTATAAACATGAATAGTGAAGAGAATTATAAGATTTATATATGATATATAGTTAGACCTTCATTTATCCTCTCTTTATATGTCATTTGTTTTTTCACAAATTTATCTTCCTTGCAATCATGGTAAAAATGTGGTAGTTAAAAAAAACATACACAATTAAAAAAAAAAAAAGTGGTTAACAAGCGACCTAAGTTTTTAAAAACTATTTTAACACCTATCTTCCTTTATTTTTATTTTTAATTCTTTAAAAATACTTTAGGGATCTCATACTTAAAAAAAAATGAAAACAACTCTTTATTATCAGCGCTGTTAACCATTATTTTTAATTTTAAAGTAGATTAGCATTATTTTCAAAACACAATTAAATTTTTTTTTTCTTAAATCAAACGCATTGAATTTTAAGTGTTTTTTTTTTTCTGTTCTTTAAAATATATTTATATTTTTCAAAATCTCAAATTGTTTAACAGTCCATGTATAAACTTCTCAGGTACATACTCCCCATGAGCATGCATCTCCTCTAACAACATATATATTTCTCACTAATATCTCTATTTACGAACTTTCCCTCCTCTTTTTCCTTGATTCTTCTTTAATATAATGGTATGCAAACATAAAAAAAAAACTTTATTAATTTCCATTTCTATAATTAAAAAATAAAATTACAACCGAATTGTTCTTATATAATTAATAAATCAACATAAGCAAAAAATCGAGTTTTATATTTGAAATCGAACCATTTGTTGTTCTTTGATATTTCCAGACTTCCAAGATGTAGCTTCGACTCAAGCATTCACGTTTCAGAACACCGCGGCCACAGACCCAAACGTAACAATAGTGGCATTTAGAGGTACTACACTCAACAACTTTGTGGATATAAAGGAAGATATCAATTGCTCTTGGTACAACATCAAAGGCATTGGCAACATCCACAGCGGCTTCATGCGAGCCCTCGGCCTCCAAAAGGCCACTGGCTGGCCAAAGGACCTCCCTCCAAGCCCTGACAATCACCAATTCGCCTACTACACCCTTCGCCAACAACTCAAAGACATTGTCAAAGCCAATGACAAGGCAAGGTTCATCTTCACTGGCCACAGCTTGGGCGGAGCCCTCGCCATTCTCTTTGTCACTATCCTCGCCTACCACGGCGAGTCTGATCTTCTACAAAGGCTGCAAGCCGTTTACACCTTCGGCCAACCCAGGATCGGCAACTCCGACTGTGCTAACTTTATGGAACAAACTATCAAAACATATGGCTTCAAATATTACAGATACGTTTACTCTTACGACGTAGTGCCCAGAGTTCCTTTCGATTTGTGGAATTTATGGTACAAACACTTCGGCGGATGCGTCTACTTCAACTGCTGCTACAAAGGGGAGGTAATTAAATTACTTACAACCTCCTATAAAGAAAATTAATTTATTCAACTTGTAATTTAACAATTACAAATCTTGGGTTGAATGCAGTTTTTGGAGACTGACGAACCAGACAAGAACTACTGTTTGGAGTTTTGGCTGATACCATGGATCCATATCGTTGCATGTTGGGAGCTTATAAGAAGTTTAATAAACCCAATCTTCAAGGGTTTTGATTACTTTGAAGGTTTCGGTCCTTTACTGATTAGGCTGTTTGGGTTGATTTTTCCAGGAGTATCAGCTCACATATGTCCGAATTATATTAATACCACCCGCTATGGAAAGATTCAACCATCCAAGGACGACATGGACAAGATGAAGATCAGAGATATAATATGTTTGTAATCTGTCCTTAATATTTATCTTCATGTGCTTTATTTTACTCCCAAGTCATCAATCGCATATTTTAATATGGAATAAAGGCTAGGGCTACTGTTTGCTTGTTAGCATATATATTTGAAGGTGTTTTAGATTTCTTTAAGTATAGAACATTATGAATAATTTAGGTTTGTAATAACTTGTGTCCTTTAATTATAAAATCTAGGTTTATGTGTGTGTGTAACTTAGGGTTATGTATAATTTCTAGGGTTAAGCATATATCCAAATTTAATTTGGAATATGCTATTTCCTAATCTTATGTTACACAAATCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAAAAAGTGTGCTTTGATTTTATGTGAGTACTTCAATAAATAATACTTCATATTATAAAGTTTCGTTCTAATATGTCTTAAAATTGTTACTCTTTAATCTAACTAAAGTGATTTTAATTTAATTGCGATA

mRNA sequence

ATGGGTCCTGAAGATCACCAATTTAGTGATGATTATCTGATATTGAAGCCTGAGAGTGTAAGTTGGTTTGATCTGTTTCTCTTCCTTTTTCCTTTCGGTTTCGTAGACATCAGAGCGTTGGCTGACATCCCCGAAGGCAAAGAAGATTCTTACAATAGCTTTGGAGTCCGATGGGTTATCTTCGTCTCCGTTTTCTTGCAGAAACTCCTTGATGCCATCGCCAATCTCTTCAAACTATTCAAATTTTTCCGTCGTATTTTTACCAAGAGAGGAAAGTTCTCGGGGATGCCTGGTAAAACTTACGCAACGGAGGTGAAATGTAGAGATTGGAGAATAGAAGTGGCCGTGGGGAAGAGGAAGAACATAAAATACGGTGAGGAGGATTTCAGATTTGATGGTTCCCTAACCATGATGGCTTCCGCTTTAGCCTACGAAGACTATTCACCAGCCCCCTCCGTCGTCCAGACCGTCGTAAATAACTGCTGGAAGATGAAGCTACTTGACTGCTACGATGATTTTTGGAATGACTTCCAAGATGTAGCTTCGACTCAAGCATTCACGTTTCAGAACACCGCGGCCACAGACCCAAACGTAACAATAGTGGCATTTAGAGGTACTACACTCAACAACTTTGTGGATATAAAGGAAGATATCAATTGCTCTTGGTACAACATCAAAGGCATTGGCAACATCCACAGCGGCTTCATGCGAGCCCTCGGCCTCCAAAAGGCCACTGGCTGGCCAAAGGACCTCCCTCCAAGCCCTGACAATCACCAATTCGCCTACTACACCCTTCGCCAACAACTCAAAGACATTGTCAAAGCCAATGACAAGGCAAGGTTCATCTTCACTGGCCACAGCTTGGGCGGAGCCCTCGCCATTCTCTTTGTCACTATCCTCGCCTACCACGGCGAGTCTGATCTTCTACAAAGGCTGCAAGCCGTTTACACCTTCGGCCAACCCAGGATCGGCAACTCCGACTGTGCTAACTTTATGGAACAAACTATCAAAACATATGGCTTCAAATATTACAGATACGTTTACTCTTACGACGTAGTGCCCAGAGTTCCTTTCGATTTGTGGAATTTATGGTACAAACACTTCGGCGGATGCGTCTACTTCAACTGCTGCTACAAAGGGGAGTTTTTGGAGACTGACGAACCAGACAAGAACTACTGTTTGGAGTTTTGGCTGATACCATGGATCCATATCGTTGCATGTTGGGAGCTTATAAGAAGTTTAATAAACCCAATCTTCAAGGGTTTTGATTACTTTGAAGGTTTCGGTCCTTTACTGATTAGGCTGTTTGGGTTGATTTTTCCAGGAGTATCAGCTCACATATGTCCGAATTATATTAATACCACCCGCTATGGAAAGATTCAACCATCCAAGGACGACATGGACAAGATGAAGATCAGAGATATAATATGTTTGTAA

Coding sequence (CDS)

ATGGGTCCTGAAGATCACCAATTTAGTGATGATTATCTGATATTGAAGCCTGAGAGTGTAAGTTGGTTTGATCTGTTTCTCTTCCTTTTTCCTTTCGGTTTCGTAGACATCAGAGCGTTGGCTGACATCCCCGAAGGCAAAGAAGATTCTTACAATAGCTTTGGAGTCCGATGGGTTATCTTCGTCTCCGTTTTCTTGCAGAAACTCCTTGATGCCATCGCCAATCTCTTCAAACTATTCAAATTTTTCCGTCGTATTTTTACCAAGAGAGGAAAGTTCTCGGGGATGCCTGGTAAAACTTACGCAACGGAGGTGAAATGTAGAGATTGGAGAATAGAAGTGGCCGTGGGGAAGAGGAAGAACATAAAATACGGTGAGGAGGATTTCAGATTTGATGGTTCCCTAACCATGATGGCTTCCGCTTTAGCCTACGAAGACTATTCACCAGCCCCCTCCGTCGTCCAGACCGTCGTAAATAACTGCTGGAAGATGAAGCTACTTGACTGCTACGATGATTTTTGGAATGACTTCCAAGATGTAGCTTCGACTCAAGCATTCACGTTTCAGAACACCGCGGCCACAGACCCAAACGTAACAATAGTGGCATTTAGAGGTACTACACTCAACAACTTTGTGGATATAAAGGAAGATATCAATTGCTCTTGGTACAACATCAAAGGCATTGGCAACATCCACAGCGGCTTCATGCGAGCCCTCGGCCTCCAAAAGGCCACTGGCTGGCCAAAGGACCTCCCTCCAAGCCCTGACAATCACCAATTCGCCTACTACACCCTTCGCCAACAACTCAAAGACATTGTCAAAGCCAATGACAAGGCAAGGTTCATCTTCACTGGCCACAGCTTGGGCGGAGCCCTCGCCATTCTCTTTGTCACTATCCTCGCCTACCACGGCGAGTCTGATCTTCTACAAAGGCTGCAAGCCGTTTACACCTTCGGCCAACCCAGGATCGGCAACTCCGACTGTGCTAACTTTATGGAACAAACTATCAAAACATATGGCTTCAAATATTACAGATACGTTTACTCTTACGACGTAGTGCCCAGAGTTCCTTTCGATTTGTGGAATTTATGGTACAAACACTTCGGCGGATGCGTCTACTTCAACTGCTGCTACAAAGGGGAGTTTTTGGAGACTGACGAACCAGACAAGAACTACTGTTTGGAGTTTTGGCTGATACCATGGATCCATATCGTTGCATGTTGGGAGCTTATAAGAAGTTTAATAAACCCAATCTTCAAGGGTTTTGATTACTTTGAAGGTTTCGGTCCTTTACTGATTAGGCTGTTTGGGTTGATTTTTCCAGGAGTATCAGCTCACATATGTCCGAATTATATTAATACCACCCGCTATGGAAAGATTCAACCATCCAAGGACGACATGGACAAGATGAAGATCAGAGATATAATATGTTTGTAA

Protein sequence

MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQKLLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRKNIKYGEEDFRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMRALGLQKATGWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLINPIFKGFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSKDDMDKMKIRDIICL
Homology
BLAST of Spg016624 vs. NCBI nr
Match: XP_011654114.1 (uncharacterized protein LOC101203879 [Cucumis sativus] >KGN55201.1 hypothetical protein Csa_012153 [Cucumis sativus])

HSP 1 Score: 571.2 bits (1471), Expect = 8.2e-159
Identity = 283/452 (62.61%), Postives = 334/452 (73.89%), Query Frame = 0

Query: 11  DYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQKLL 70
           DYL LKPES +  DLFLF   F +VDIR L   P GKE SY SFG RW+I  S+ L KLL
Sbjct: 15  DYLTLKPESATLLDLFLFTLSFSYVDIRKLVKCPAGKERSYQSFGDRWIIVSSILLVKLL 74

Query: 71  DAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRKNIKYGEED-F 130
            AI  LF+ FK      T R K  G+P +TY  +VKC DWRIE  VGK  N K G+++ F
Sbjct: 75  IAITKLFQTFK------TMREKIFGVPQETYGAKVKCEDWRIE--VGKNSNSKLGDDNGF 134

Query: 131 RFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFTFQ 190
           R+ G+LTMMAS LAYE    +  V++TVV+NCWKM++  CY DFWNDFQD   T+AF F+
Sbjct: 135 RYYGALTMMASTLAYE----STPVIETVVDNCWKMEMDKCY-DFWNDFQDKIRTRAFAFR 194

Query: 191 NTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMRALGLQKATGWPK 250
              A DPNV +VAF+GT+     D  E++N SWYNIKGIGNIH GFM+ALGLQ+ T WPK
Sbjct: 195 ---AKDPNVMVVAFKGTSA--LGDWSENLNVSWYNIKGIGNIHDGFMQALGLQQNTDWPK 254

Query: 251 DLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGESDLL 310
           +LPP PDNH+FAYYTLRQ L+D VK NDKARFI TGHSLGGALAILFVTILA+H ES LL
Sbjct: 255 ELPPRPDNHEFAYYTLRQVLRDFVKDNDKARFIITGHSLGGALAILFVTILAFHEESALL 314

Query: 311 QRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYKHFG 370
           +RLQA+YTFGQPR G+ + A FM    K YGF YYRYVYS+D+VPRVPFD  N WYKHFG
Sbjct: 315 KRLQAIYTFGQPRSGDRNFAKFMNNLTKKYGFDYYRYVYSFDIVPRVPFDCKNFWYKHFG 374

Query: 371 GCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLINPIFKGFDYFEGFG 430
           GCVY+N CYKG+FLE  +P+ NY  E WL P+ ++ A WEL+RSL+ P+FKG  YFEGF 
Sbjct: 375 GCVYYNSCYKGKFLEA-QPNPNYFCESWLTPFQYLTAWWELLRSLVIPLFKGPKYFEGFN 434

Query: 431 PLLIRLFGLIFPGVSAHICPNYINTTRYGKIQ 462
            L++RL GL+ PGVSAH   NYIN TRYGKIQ
Sbjct: 435 TLMLRLIGLVVPGVSAHSSQNYINLTRYGKIQ 447

BLAST of Spg016624 vs. NCBI nr
Match: XP_022135571.1 (uncharacterized protein LOC111007483 isoform X2 [Momordica charantia])

HSP 1 Score: 568.9 bits (1465), Expect = 4.0e-158
Identity = 294/471 (62.42%), Postives = 351/471 (74.52%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           MGPED+QFS+DYLILKPE  +  DLFLF+ PFG++ +R L D PE KE+SY SFG RWVI
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  FVSVFLQKLLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRK 120
           F+S+  QK L AIANL + ++        RGKF  +  +TYA+EV CRDW+I    GK +
Sbjct: 61  FISILSQKFLLAIANLLEKWE------PMRGKFWRV--QTYASEVNCRDWKI----GKEE 120

Query: 121 NIKYGEED--FRFDGSLTMMASALAYEDYS-PAPSVVQTVVNNCWKMKLLDCYDDFWNDF 180
           NIK   +D  F + G++T+MAS L Y+D S P  SVV TVVN+CWKMKLL CY DF N F
Sbjct: 121 NIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCY-DFENMF 180

Query: 181 QDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNI-KGIGNIHSGFM 240
           +  A TQA  FQNT ATDPNVT+VAF+GT  N   D   D+N SWYN+ + IGNIHSGFM
Sbjct: 181 ESKARTQATMFQNT-ATDPNVTVVAFKGT--NTIYDAILDLNFSWYNVDQRIGNIHSGFM 240

Query: 241 RALGLQKAT-GWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAIL 300
            ALGLQK T GWPK+LP S  +HQFAYY LRQQL+DI K+NDKA+FIFTGHSLGGALAIL
Sbjct: 241 EALGLQKGTIGWPKELPKS--DHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAIL 300

Query: 301 FVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPR 360
           FVTIL+YH ESD+L +LQAVYTFGQPR+GNS    FME T K Y FKYYRYVYS D+VPR
Sbjct: 301 FVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSMDLVPR 360

Query: 361 VPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLI 420
           +PFD+++ WYKHFGGCVYFNCCY G+F+E  +P+KNY    WLIP  ++ A WELIRSL+
Sbjct: 361 IPFDIFDEWYKHFGGCVYFNCCYSGQFMEV-QPNKNYFSPLWLIP-KYLFALWELIRSLM 420

Query: 421 NPIFK-GFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSKD 466
            P+ K    YFEGF  LL+R FGL+ PG SAH+C NYI   R GK+ P KD
Sbjct: 421 MPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGKMPPLKD 451

BLAST of Spg016624 vs. NCBI nr
Match: XP_022139747.1 (uncharacterized protein LOC111010582 [Momordica charantia])

HSP 1 Score: 565.8 bits (1457), Expect = 3.4e-157
Identity = 295/488 (60.45%), Postives = 359/488 (73.57%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           MG ++++FS DYLILKPE  +   LFLF  PFG+  +R L D P  KE+SY +FG RWVI
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  FVSVFLQKLLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYAT-EVKCRDWRIEVAVGKR 120
           F+S+ LQK L AIANL K  K      + RGKF     KT A  +V C++W+I   +G  
Sbjct: 61  FISILLQKFLLAIANLIKYCK------SMRGKFH----KTCAAPKVNCKEWKI---IGME 120

Query: 121 KNIKYGEED-FRFDGSLTMMASALAYEDY-SPAPSVVQTVVNNCWKMKLLDCYDDFWNDF 180
           KNIK  ++D F++ G+LT+MAS LAY+DY SP PSVVQTVVN CWKM LL CY DFWNDF
Sbjct: 121 KNIKLADDDTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLGCY-DFWNDF 180

Query: 181 QDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMR 240
           Q+ A+TQ F FQNT ATDPNVT+VAFRG+  +   D   D+N SWYNI+GIG IH GFM+
Sbjct: 181 QNKATTQVFMFQNT-ATDPNVTVVAFRGS--SEIYDWMVDLNLSWYNIEGIGKIHGGFMQ 240

Query: 241 ALGLQKATGWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFV 300
           ALGLQK TGWPK+L  +PD H FAYYTLRQQL+DIVK+ND ARFIFTGHSLGGALA+LF 
Sbjct: 241 ALGLQKGTGWPKEL-ANPD-HDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGALAVLFA 300

Query: 301 TILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVP 360
           T+LA+H ++ LL++LQAVYTFGQPR+G+   A FME TI+ YGFKYYRYVYS D+VPRVP
Sbjct: 301 TLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVYSSDLVPRVP 360

Query: 361 FDL-WNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLIN 420
           FD   NLWY+HFGGCVYFNC Y G+FLE  +P+KNY    WLI   ++ A WELIRSL+ 
Sbjct: 361 FDFNDNLWYEHFGGCVYFNCSYNGKFLEV-QPNKNYFSPIWLIS-KYVSAWWELIRSLMI 420

Query: 421 PIFK-GFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSK--------DDM 476
           P+ K   DY EGF  LL+RLFGL+FPG SAH+C NYIN+ R+GK    K         ++
Sbjct: 421 PVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWGKCNHEKTLIPAAEHQNV 467

BLAST of Spg016624 vs. NCBI nr
Match: XP_008452564.1 (PREDICTED: uncharacterized protein LOC103493550 [Cucumis melo] >KAA0064391.1 triacylglycerol lipase [Cucumis melo var. makuwa] >TYK20195.1 triacylglycerol lipase [Cucumis melo var. makuwa])

HSP 1 Score: 564.3 bits (1453), Expect = 1.0e-156
Identity = 288/455 (63.30%), Postives = 336/455 (73.85%), Query Frame = 0

Query: 9   SDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQK 68
           S+DYL LKPES ++ +LFLF   F FVDIR LAD P GKE SY SFG RW+I  S+ LQK
Sbjct: 3   SNDYLKLKPESATFLNLFLFTLSFNFVDIRTLADCPAGKERSYQSFGDRWIIVSSILLQK 62

Query: 69  LLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRKNIKYGEED 128
           LL AIAN  + FK        R K  G+P +TY  +VKC DWRIEV    + N+K G+++
Sbjct: 63  LLLAIANHLQTFKII------RAKIFGVPQETYGGKVKCGDWRIEVG---KNNLKLGDDN 122

Query: 129 -FRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFT 188
            FR+ G+LTMMAS LAYE    +PSV+QTVVNNCWKM LL CY DFWNDFQ   +TQAF 
Sbjct: 123 GFRYYGALTMMASTLAYE----SPSVIQTVVNNCWKMNLLKCY-DFWNDFQGKPTTQAFA 182

Query: 189 FQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMRALGLQK-ATG 248
           FQNT A DPNV +VAFRG+  +   D   D N SWYNI+GIG+IH GFM+ALGLQ     
Sbjct: 183 FQNT-ANDPNVIVVAFRGS--SELGDWLVDFNVSWYNIEGIGHIHYGFMQALGLQNDGID 242

Query: 249 WPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGES 308
           WPK+LP  PDNHQFAYYTLRQ L+D+VKANDKARFI TGHSLGGALA LFVTILA+HGES
Sbjct: 243 WPKELPSRPDNHQFAYYTLRQVLRDVVKANDKARFIITGHSLGGALATLFVTILAFHGES 302

Query: 309 DLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYK 368
            LL+RLQA+YTFGQPR G+ + A FM    K YGF YYRYVYS+D+V RVPFD  N WYK
Sbjct: 303 ALLKRLQAIYTFGQPRAGDRNFAQFMNNLTKNYGFGYYRYVYSFDMVARVPFDFKNSWYK 362

Query: 369 HFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLINPIFKGFDYFE 428
           HFG CVY+N CYKG+FLE  EP+ NY  E  LIP  ++ A WEL+RSL+ P FKG    E
Sbjct: 363 HFGECVYYNSCYKGKFLEA-EPNPNYFFEILLIPRKYLTAWWELLRSLVIPPFKG----E 422

Query: 429 GFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQ 462
           GF  L++RLFGL+ PG SAH+  NYIN+TRYGKIQ
Sbjct: 423 GFNTLMVRLFGLLLPGASAHLTQNYINSTRYGKIQ 435

BLAST of Spg016624 vs. NCBI nr
Match: XP_022135562.1 (uncharacterized protein LOC111007483 isoform X1 [Momordica charantia])

HSP 1 Score: 543.9 bits (1400), Expect = 1.4e-150
Identity = 283/471 (60.08%), Postives = 342/471 (72.61%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           MG  +++FS+DYL LKP+  +  DLF+FL PFG + +R L D P  KE+SY SF  RWVI
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 61  FVSVFLQKLLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRK 120
           F S+  QK L A+ANL + ++      +   K    P +TYA+EV CRDW+I    GK +
Sbjct: 61  FFSILSQKSLFALANLLEKWE------SSTEKLRTAP-QTYASEVNCRDWKI----GKEE 120

Query: 121 NIKYGEED--FRFDGSLTMMASALAYEDYS-PAPSVVQTVVNNCWKMKLLDCYDDFWNDF 180
           NIK   +D  F + G++T+MAS L Y+D S P  SVV TVVN+CWKMKLL CY DF N F
Sbjct: 121 NIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCY-DFENMF 180

Query: 181 QDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNI-KGIGNIHSGFM 240
           +  A TQA  FQNT ATDPNVT+VAF+GT  N   D   D+N SWYN+ + IGNIHSGFM
Sbjct: 181 ESKARTQATMFQNT-ATDPNVTVVAFKGT--NTIYDAILDLNFSWYNVDQRIGNIHSGFM 240

Query: 241 RALGLQKAT-GWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAIL 300
            ALGLQK T GWPK+LP S  +HQFAYY LRQQL+DI K+NDKA+FIFTGHSLGGALAIL
Sbjct: 241 EALGLQKGTIGWPKELPKS--DHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAIL 300

Query: 301 FVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPR 360
           FVTIL+YH ESD+L +LQAVYTFGQPR+GNS    FME T K Y FKYYRYVYS D+VPR
Sbjct: 301 FVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSMDLVPR 360

Query: 361 VPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLI 420
           +PFD+++ WYKHFGGCVYFNCCY G+F+E  +P+KNY    WLIP  ++ A WELIRSL+
Sbjct: 361 IPFDIFDEWYKHFGGCVYFNCCYSGQFMEV-QPNKNYFSPLWLIP-KYLFALWELIRSLM 420

Query: 421 NPIFK-GFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSKD 466
            P+ K    YFEGF  LL+R FGL+ PG SAH+C NYI   R GK+ P KD
Sbjct: 421 MPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGKMPPLKD 452

BLAST of Spg016624 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 5.8e-67
Identity = 167/503 (33.20%), Postives = 255/503 (50.70%), Query Frame = 0

Query: 11  DYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGV-----RWVIFVSVF 70
           +YLI++P    + DLF +             ++P+ +E S  +        RWVI VS+ 
Sbjct: 18  NYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWVIVVSIL 77

Query: 71  LQKLLDAIANLFK----LFKFFRRIFTKRGKFSGM-----------PGKTYATEVKC--- 130
           ++K++  +    +    +  FF  +F+  G F G+           P +   T V     
Sbjct: 78  VRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTFVSTIGQ 137

Query: 131 --------RDWR-------IEVAVGKRKNIKYGEEDFRFDGSLTMMASALAYEDYSPAPS 190
                   ++W        I+     R  I+ G         L +MAS LAYE+      
Sbjct: 138 LDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLM---DLCVMASKLAYEN----AK 197

Query: 191 VVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFV 250
           VV+ VV+  WKM L++ + D WND+Q   STQ F F +    D N+ +++FRGT   +  
Sbjct: 198 VVENVVDLHWKMNLVE-FLDCWNDYQKQMSTQVFVFTD-KQKDANLIVISFRGTEPFDAD 257

Query: 251 DIKEDINCSWYNIKGIGNIHSGFMRALGL---QKATGWPKDLPPSPDNHQ---------- 310
           D   D + SWY +  +G +H GF+ A+GL      T +  +L     + +          
Sbjct: 258 DWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDM 317

Query: 311 ---FAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGESDLLQRLQAVY 370
               AYY +R  LK ++  ++ ARF+ TGHSLGGALAILF T+L  + E+++++RL  VY
Sbjct: 318 VERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVY 377

Query: 371 TFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYKHFGGCVYFNC 430
           TFGQPRIGN +   FM+  +     +Y+R VY  D+VPR+P+D     YKHFG C++++ 
Sbjct: 378 TFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDS 437

Query: 431 CYKGEFLETDEPDKN-YCLEFWLIPWIHIVACWELIRSLINPIFKGFDYFEGFGPLLIRL 459
            Y  E    DEPD N Y L + ++   H++A WEL+R L      G DY EG+  +L RL
Sbjct: 438 FY-NETKAEDEPDPNPYGLRYKILG--HVIAVWELVRGLTMGYTHGPDYKEGWFRILFRL 497

BLAST of Spg016624 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.5e-65
Identity = 166/491 (33.81%), Postives = 241/491 (49.08%), Query Frame = 0

Query: 57  RWVIFVSVFLQKLLDAIANLFK----LFKFFRRIFTKRGKFSG-----------MP---G 116
           RWVIFVS+ ++KL+       +    L +FF  +F+  G F G           MP    
Sbjct: 86  RWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPHRGS 145

Query: 117 KTYATEVKCRDWRIEVAVGKRKNIKYGEEDF--------RFDGSLTMMASALAYEDYSPA 176
           +T+ + +   D RI +   +    + GE DF        R    L MMAS LAYE+    
Sbjct: 146 ETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYEN---- 205

Query: 177 PSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFTFQNTAATDPNVTIVAFRGTTLNN 236
             VV+ VVN  WKM  +D Y + WNDF+   STQ F   +    D N+ +V+FRGT   +
Sbjct: 206 EKVVRNVVNLHWKMHFVDFY-NCWNDFEKEMSTQVFLLCD-KPKDANLILVSFRGTEPFD 265

Query: 237 FVDIKEDINCSWYNIKGIGNIHSGFMRALGLQKATGWP--------------------KD 296
             D   D + SWY I  +G +H GF+ ALGL   T                         
Sbjct: 266 ADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHAT 325

Query: 297 LPPSPDN-------------------------------------HQFAYYTLRQQLKDIV 356
           +PPS  +                                      + AYY +R +LK ++
Sbjct: 326 IPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLL 385

Query: 357 KANDKARFIFTGHSLGGALAILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFME 416
           K +  A+F+ TGHSLGGALAILF  +L  H E D+++RL  +YT+GQPR+GN     FME
Sbjct: 386 KEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFME 445

Query: 417 QTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYC 465
             ++    KY+R VY  D+VPR+P+D     +KHFG C Y+N  Y  + +  +EP+ NY 
Sbjct: 446 AHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNI-NEEPNPNYF 505

BLAST of Spg016624 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 2.0e-51
Identity = 130/348 (37.36%), Postives = 187/348 (53.74%), Query Frame = 0

Query: 135 LTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFTFQNTAAT 194
           L +MAS LAYE+      VV+ VV   WKM  +  Y    N FQD  +T AF F +    
Sbjct: 172 LCIMASKLAYEN----TKVVERVVAEHWKMHFVADYGGM-NYFQDARNTHAFIFCD-KPK 231

Query: 195 DPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMRALGL------------- 254
           D N+ +++FRGT   +  +   D + S   +   G++H GF+ A+GL             
Sbjct: 232 DANLIVISFRGTGPFSIPNWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSI 291

Query: 255 -----QKATGWPKDLPPSPDNHQFA----YYTLRQQLKDIVKANDKARFIFTGHSLGGAL 314
                   T   K+   +PD+  +A    Y+     LK ++K +  A+F+ TGHSLGGAL
Sbjct: 292 NTKSPGSITELRKESEMAPDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGAL 351

Query: 315 AILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDV 374
           AILF  IL    E+++L RL  VYTFGQPRIGN +   FM+  +     +Y+R VY  D+
Sbjct: 352 AILFTCILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDM 411

Query: 375 VPRVPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKN-YCLEFWLIPWIHIVACWELI 434
           VPRVPFD     ++HFG C+Y++  + G F + +EP +N + +E  +    HI A WEL 
Sbjct: 412 VPRVPFDDVFFTFEHFGTCIYYDSRFFGYFTK-EEPSRNPFGIENAIS--AHITAWWELW 471

Query: 435 RSLINPIFKGFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGK 460
           RS I     G +Y E +   + R+ GL  PGV+AH   NY+N+ R G+
Sbjct: 472 RSFILNHVYGAEYKETWESRMFRILGLFLPGVAAHSPVNYVNSVRLGR 510

BLAST of Spg016624 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.5e-09
Identity = 38/94 (40.43%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 264 TLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGESDLLQRLQAVYTFGQPRI 323
           TLRQ+++D V+ +   R +FTGHSLGGALA +    L  +G          V+++G PR+
Sbjct: 145 TLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYD------IDVFSYGAPRV 204

Query: 324 GNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVP 358
           GN   A F+  T++T G   YR  ++ D+VPR+P
Sbjct: 205 GNRAFAEFL--TVQT-GGTLYRITHTNDIVPRLP 229

BLAST of Spg016624 vs. ExPASy Swiss-Prot
Match: P61871 (Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.0e-07
Identity = 55/168 (32.74%), Postives = 80/168 (47.62%), Query Frame = 0

Query: 194 TDPNVTI-VAFRGTTLNNFVDIKEDI--NCSWYN-IKGIGNIHSGFMRALGLQKATGWPK 253
           +D   TI + FRGT  N+F     DI  N S Y  +KG   +H+GF+ +        +P 
Sbjct: 193 SDKQKTIYLVFRGT--NSFRSAITDIVFNFSDYKPVKG-AKVHAGFLSSYEQVVNDYFP- 252

Query: 254 DLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGESDLL 313
                          +++QL     A+   + I TGHSLGGA A+L    L Y  E  L 
Sbjct: 253 --------------VVQEQL----TAHPTYKVIVTGHSLGGAQALLAGMDL-YQREPRLS 312

Query: 314 QRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVP 358
            +  +++T G PR+GN   A ++E T    G  + R V+  D+VP VP
Sbjct: 313 PKNLSIFTVGGPRVGNPTFAYYVEST----GIPFQRTVHKRDIVPHVP 333

BLAST of Spg016624 vs. ExPASy TrEMBL
Match: A0A0A0L4W4 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639890 PE=4 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 4.0e-159
Identity = 283/452 (62.61%), Postives = 334/452 (73.89%), Query Frame = 0

Query: 11  DYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQKLL 70
           DYL LKPES +  DLFLF   F +VDIR L   P GKE SY SFG RW+I  S+ L KLL
Sbjct: 15  DYLTLKPESATLLDLFLFTLSFSYVDIRKLVKCPAGKERSYQSFGDRWIIVSSILLVKLL 74

Query: 71  DAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRKNIKYGEED-F 130
            AI  LF+ FK      T R K  G+P +TY  +VKC DWRIE  VGK  N K G+++ F
Sbjct: 75  IAITKLFQTFK------TMREKIFGVPQETYGAKVKCEDWRIE--VGKNSNSKLGDDNGF 134

Query: 131 RFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFTFQ 190
           R+ G+LTMMAS LAYE    +  V++TVV+NCWKM++  CY DFWNDFQD   T+AF F+
Sbjct: 135 RYYGALTMMASTLAYE----STPVIETVVDNCWKMEMDKCY-DFWNDFQDKIRTRAFAFR 194

Query: 191 NTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMRALGLQKATGWPK 250
              A DPNV +VAF+GT+     D  E++N SWYNIKGIGNIH GFM+ALGLQ+ T WPK
Sbjct: 195 ---AKDPNVMVVAFKGTSA--LGDWSENLNVSWYNIKGIGNIHDGFMQALGLQQNTDWPK 254

Query: 251 DLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGESDLL 310
           +LPP PDNH+FAYYTLRQ L+D VK NDKARFI TGHSLGGALAILFVTILA+H ES LL
Sbjct: 255 ELPPRPDNHEFAYYTLRQVLRDFVKDNDKARFIITGHSLGGALAILFVTILAFHEESALL 314

Query: 311 QRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYKHFG 370
           +RLQA+YTFGQPR G+ + A FM    K YGF YYRYVYS+D+VPRVPFD  N WYKHFG
Sbjct: 315 KRLQAIYTFGQPRSGDRNFAKFMNNLTKKYGFDYYRYVYSFDIVPRVPFDCKNFWYKHFG 374

Query: 371 GCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLINPIFKGFDYFEGFG 430
           GCVY+N CYKG+FLE  +P+ NY  E WL P+ ++ A WEL+RSL+ P+FKG  YFEGF 
Sbjct: 375 GCVYYNSCYKGKFLEA-QPNPNYFCESWLTPFQYLTAWWELLRSLVIPLFKGPKYFEGFN 434

Query: 431 PLLIRLFGLIFPGVSAHICPNYINTTRYGKIQ 462
            L++RL GL+ PGVSAH   NYIN TRYGKIQ
Sbjct: 435 TLMLRLIGLVVPGVSAHSSQNYINLTRYGKIQ 447

BLAST of Spg016624 vs. ExPASy TrEMBL
Match: A0A6J1C148 (uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 2.0e-158
Identity = 294/471 (62.42%), Postives = 351/471 (74.52%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           MGPED+QFS+DYLILKPE  +  DLFLF+ PFG++ +R L D PE KE+SY SFG RWVI
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  FVSVFLQKLLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRK 120
           F+S+  QK L AIANL + ++        RGKF  +  +TYA+EV CRDW+I    GK +
Sbjct: 61  FISILSQKFLLAIANLLEKWE------PMRGKFWRV--QTYASEVNCRDWKI----GKEE 120

Query: 121 NIKYGEED--FRFDGSLTMMASALAYEDYS-PAPSVVQTVVNNCWKMKLLDCYDDFWNDF 180
           NIK   +D  F + G++T+MAS L Y+D S P  SVV TVVN+CWKMKLL CY DF N F
Sbjct: 121 NIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLGCY-DFENMF 180

Query: 181 QDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNI-KGIGNIHSGFM 240
           +  A TQA  FQNT ATDPNVT+VAF+GT  N   D   D+N SWYN+ + IGNIHSGFM
Sbjct: 181 ESKARTQATMFQNT-ATDPNVTVVAFKGT--NTIYDAILDLNFSWYNVDQRIGNIHSGFM 240

Query: 241 RALGLQKAT-GWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAIL 300
            ALGLQK T GWPK+LP S  +HQFAYY LRQQL+DI K+NDKA+FIFTGHSLGGALAIL
Sbjct: 241 EALGLQKGTIGWPKELPKS--DHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGGALAIL 300

Query: 301 FVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPR 360
           FVTIL+YH ESD+L +LQAVYTFGQPR+GNS    FME T K Y FKYYRYVYS D+VPR
Sbjct: 301 FVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSMDLVPR 360

Query: 361 VPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLI 420
           +PFD+++ WYKHFGGCVYFNCCY G+F+E  +P+KNY    WLIP  ++ A WELIRSL+
Sbjct: 361 IPFDIFDEWYKHFGGCVYFNCCYSGQFMEV-QPNKNYFSPLWLIP-KYLFALWELIRSLM 420

Query: 421 NPIFK-GFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSKD 466
            P+ K    YFEGF  LL+R FGL+ PG SAH+C NYI   R GK+ P KD
Sbjct: 421 MPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGKMPPLKD 451

BLAST of Spg016624 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.7e-157
Identity = 295/488 (60.45%), Postives = 359/488 (73.57%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           MG ++++FS DYLILKPE  +   LFLF  PFG+  +R L D P  KE+SY +FG RWVI
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  FVSVFLQKLLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYAT-EVKCRDWRIEVAVGKR 120
           F+S+ LQK L AIANL K  K      + RGKF     KT A  +V C++W+I   +G  
Sbjct: 61  FISILLQKFLLAIANLIKYCK------SMRGKFH----KTCAAPKVNCKEWKI---IGME 120

Query: 121 KNIKYGEED-FRFDGSLTMMASALAYEDY-SPAPSVVQTVVNNCWKMKLLDCYDDFWNDF 180
           KNIK  ++D F++ G+LT+MAS LAY+DY SP PSVVQTVVN CWKM LL CY DFWNDF
Sbjct: 121 KNIKLADDDTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLGCY-DFWNDF 180

Query: 181 QDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMR 240
           Q+ A+TQ F FQNT ATDPNVT+VAFRG+  +   D   D+N SWYNI+GIG IH GFM+
Sbjct: 181 QNKATTQVFMFQNT-ATDPNVTVVAFRGS--SEIYDWMVDLNLSWYNIEGIGKIHGGFMQ 240

Query: 241 ALGLQKATGWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFV 300
           ALGLQK TGWPK+L  +PD H FAYYTLRQQL+DIVK+ND ARFIFTGHSLGGALA+LF 
Sbjct: 241 ALGLQKGTGWPKEL-ANPD-HDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGALAVLFA 300

Query: 301 TILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVP 360
           T+LA+H ++ LL++LQAVYTFGQPR+G+   A FME TI+ YGFKYYRYVYS D+VPRVP
Sbjct: 301 TLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVYSSDLVPRVP 360

Query: 361 FDL-WNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLIN 420
           FD   NLWY+HFGGCVYFNC Y G+FLE  +P+KNY    WLI   ++ A WELIRSL+ 
Sbjct: 361 FDFNDNLWYEHFGGCVYFNCSYNGKFLEV-QPNKNYFSPIWLIS-KYVSAWWELIRSLMI 420

Query: 421 PIFK-GFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSK--------DDM 476
           P+ K   DY EGF  LL+RLFGL+FPG SAH+C NYIN+ R+GK    K         ++
Sbjct: 421 PVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWGKCNHEKTLIPAAEHQNV 467

BLAST of Spg016624 vs. ExPASy TrEMBL
Match: A0A5D3D9F9 (Triacylglycerol lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003290 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 4.8e-157
Identity = 288/455 (63.30%), Postives = 336/455 (73.85%), Query Frame = 0

Query: 9   SDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQK 68
           S+DYL LKPES ++ +LFLF   F FVDIR LAD P GKE SY SFG RW+I  S+ LQK
Sbjct: 3   SNDYLKLKPESATFLNLFLFTLSFNFVDIRTLADCPAGKERSYQSFGDRWIIVSSILLQK 62

Query: 69  LLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRKNIKYGEED 128
           LL AIAN  + FK        R K  G+P +TY  +VKC DWRIEV    + N+K G+++
Sbjct: 63  LLLAIANHLQTFKII------RAKIFGVPQETYGGKVKCGDWRIEVG---KNNLKLGDDN 122

Query: 129 -FRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFT 188
            FR+ G+LTMMAS LAYE    +PSV+QTVVNNCWKM LL CY DFWNDFQ   +TQAF 
Sbjct: 123 GFRYYGALTMMASTLAYE----SPSVIQTVVNNCWKMNLLKCY-DFWNDFQGKPTTQAFA 182

Query: 189 FQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMRALGLQK-ATG 248
           FQNT A DPNV +VAFRG+  +   D   D N SWYNI+GIG+IH GFM+ALGLQ     
Sbjct: 183 FQNT-ANDPNVIVVAFRGS--SELGDWLVDFNVSWYNIEGIGHIHYGFMQALGLQNDGID 242

Query: 249 WPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGES 308
           WPK+LP  PDNHQFAYYTLRQ L+D+VKANDKARFI TGHSLGGALA LFVTILA+HGES
Sbjct: 243 WPKELPSRPDNHQFAYYTLRQVLRDVVKANDKARFIITGHSLGGALATLFVTILAFHGES 302

Query: 309 DLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYK 368
            LL+RLQA+YTFGQPR G+ + A FM    K YGF YYRYVYS+D+V RVPFD  N WYK
Sbjct: 303 ALLKRLQAIYTFGQPRAGDRNFAQFMNNLTKNYGFGYYRYVYSFDMVARVPFDFKNSWYK 362

Query: 369 HFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLINPIFKGFDYFE 428
           HFG CVY+N CYKG+FLE  EP+ NY  E  LIP  ++ A WEL+RSL+ P FKG    E
Sbjct: 363 HFGECVYYNSCYKGKFLEA-EPNPNYFFEILLIPRKYLTAWWELLRSLVIPPFKG----E 422

Query: 429 GFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQ 462
           GF  L++RLFGL+ PG SAH+  NYIN+TRYGKIQ
Sbjct: 423 GFNTLMVRLFGLLLPGASAHLTQNYINSTRYGKIQ 435

BLAST of Spg016624 vs. ExPASy TrEMBL
Match: A0A1S3BVA9 (uncharacterized protein LOC103493550 OS=Cucumis melo OX=3656 GN=LOC103493550 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 4.8e-157
Identity = 288/455 (63.30%), Postives = 336/455 (73.85%), Query Frame = 0

Query: 9   SDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQK 68
           S+DYL LKPES ++ +LFLF   F FVDIR LAD P GKE SY SFG RW+I  S+ LQK
Sbjct: 3   SNDYLKLKPESATFLNLFLFTLSFNFVDIRTLADCPAGKERSYQSFGDRWIIVSSILLQK 62

Query: 69  LLDAIANLFKLFKFFRRIFTKRGKFSGMPGKTYATEVKCRDWRIEVAVGKRKNIKYGEED 128
           LL AIAN  + FK        R K  G+P +TY  +VKC DWRIEV    + N+K G+++
Sbjct: 63  LLLAIANHLQTFKII------RAKIFGVPQETYGGKVKCGDWRIEVG---KNNLKLGDDN 122

Query: 129 -FRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDDFWNDFQDVASTQAFT 188
            FR+ G+LTMMAS LAYE    +PSV+QTVVNNCWKM LL CY DFWNDFQ   +TQAF 
Sbjct: 123 GFRYYGALTMMASTLAYE----SPSVIQTVVNNCWKMNLLKCY-DFWNDFQGKPTTQAFA 182

Query: 189 FQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIHSGFMRALGLQK-ATG 248
           FQNT A DPNV +VAFRG+  +   D   D N SWYNI+GIG+IH GFM+ALGLQ     
Sbjct: 183 FQNT-ANDPNVIVVAFRGS--SELGDWLVDFNVSWYNIEGIGHIHYGFMQALGLQNDGID 242

Query: 249 WPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFIFTGHSLGGALAILFVTILAYHGES 308
           WPK+LP  PDNHQFAYYTLRQ L+D+VKANDKARFI TGHSLGGALA LFVTILA+HGES
Sbjct: 243 WPKELPSRPDNHQFAYYTLRQVLRDVVKANDKARFIITGHSLGGALATLFVTILAFHGES 302

Query: 309 DLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYSYDVVPRVPFDLWNLWYK 368
            LL+RLQA+YTFGQPR G+ + A FM    K YGF YYRYVYS+D+V RVPFD  N WYK
Sbjct: 303 ALLKRLQAIYTFGQPRAGDRNFAQFMNNLTKNYGFGYYRYVYSFDMVARVPFDFKNSWYK 362

Query: 369 HFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWELIRSLINPIFKGFDYFE 428
           HFG CVY+N CYKG+FLE  EP+ NY  E  LIP  ++ A WEL+RSL+ P FKG    E
Sbjct: 363 HFGECVYYNSCYKGKFLEA-EPNPNYFFEILLIPRKYLTAWWELLRSLVIPPFKG----E 422

Query: 429 GFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQ 462
           GF  L++RLFGL+ PG SAH+  NYIN+TRYGKIQ
Sbjct: 423 GFNTLMVRLFGLLLPGASAHLTQNYINSTRYGKIQ 435

BLAST of Spg016624 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 337.4 bits (864), Expect = 1.8e-92
Identity = 194/494 (39.27%), Postives = 285/494 (57.69%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           M   + +F + Y ++ P   S+ DL L LF       R +   P+  +    SF  RW++
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWIL 60

Query: 61  FVSVFLQKLLDAIANLF----KLFKFFRRIFTKRGKFSG-----MPGK---------TYA 120
            +++FLQK+L  ++  F    +   ++  + T  G F       M GK         TY 
Sbjct: 61  ALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYT 120

Query: 121 TEVKCRDWRIEVAVGKRKNIKYGEEDFRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCW 180
           + + C D RIE+     + I  G  +++    L++MAS ++YE    +   + +VV N W
Sbjct: 121 SFIGCSDRRIEL----DEKINVGSIEYK--SMLSIMASKISYE----SKPYITSVVKNTW 180

Query: 181 KMKLLDCYDDFWNDFQDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSW 240
           KM L+  Y DF+N FQ+   TQAF F+ T++T+P++ +V+FRGT      D   D++ SW
Sbjct: 181 KMDLVGNY-DFYNAFQESKLTQAFVFK-TSSTNPDLIVVSFRGTEPFEAADWCTDLDLSW 240

Query: 241 YNIKGIGNIHSGFMRALGLQKATGWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFI 300
           Y +K +G +H+GF RALGLQK  GWPK+       HQ+AYYT+RQ L+D +  N   ++I
Sbjct: 241 YEMKNVGKVHAGFSRALGLQK-DGWPKE--NISLLHQYAYYTIRQMLRDKLGRNKNLKYI 300

Query: 301 FTGHSLGGALAILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFK 360
            TGHSLGGALA LF  ILA HGE +LL +L+ +YTFGQPR+G+ D   FM+  +K +G +
Sbjct: 301 LTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIE 360

Query: 361 YYRYVYSYDVVPRVPFDLWNLW-YKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPW 420
           Y R+VY+ DVVPRVPFD   L+ YKH+G C  FN  YKG+  E D P+ NY    WLIP 
Sbjct: 361 YERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVRE-DAPNANYFNLLWLIPQ 420

Query: 421 IHIVACWELIRSLINPIFKGFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKI- 473
           + +   WE IRS I   +KG +Y E +    +R+ G++FPG S H   +Y+N+TR G + 
Sbjct: 421 L-LTGLWEFIRSFILQFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLV 477

BLAST of Spg016624 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 7.3e-89
Identity = 188/477 (39.41%), Postives = 269/477 (56.39%), Query Frame = 0

Query: 11  DYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQKLL 70
           +Y +L P   +  DL   LF     D + +    E  ED  + F  RW+IFVS+ +QKL+
Sbjct: 6   NYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQKLI 65

Query: 71  DAIANLFKLFKF----FRRIFTKRGKFSGM-----------PGKTYATEVKCR---DWRI 130
                      F    +  + +  G F  +           P K  AT        D R+
Sbjct: 66  ILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDRRV 125

Query: 131 EVAVGKRKNIKYGEEDFRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYDD 190
           E+      N K      R+   L++MAS L+YE+     + V +V++N WKM LL  Y  
Sbjct: 126 EL------NPKVERGSKRYKAMLSIMASKLSYENI----NFVSSVLHNHWKMDLLGFY-S 185

Query: 191 FWNDFQDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNIH 250
            WN +Q   ST+    ++T +TDPN+ IV+FRGT   +  D   D++ SWY +K +G IH
Sbjct: 186 CWNGYQKQRSTEVIVIKDT-STDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIH 245

Query: 251 SGFMRALGLQKATGWPKDLPPSPDNHQ---FAYYTLRQQLKDIVKANDKARFIFTGHSLG 310
            GFM+ALGLQK  GWPK++      +    +AYYT+R+ LK+I+  N  ++FI TGHSLG
Sbjct: 246 GGFMKALGLQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLG 305

Query: 311 GALAILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVYS 370
           GALAILF  +L  H E  +L+RL+ VYTFGQPR+G+ +  NFM+ ++K +  KY RYVY 
Sbjct: 306 GALAILFTAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYC 365

Query: 371 YDVVPRVPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACWE 430
            D+VPR+PFD   L +KHFG C+Y++  YKG+ +E +EP+KNY    W++P I + A WE
Sbjct: 366 NDMVPRLPFDDKTLMFKHFGACLYYDSFYKGK-VEEEEPNKNYFNLVWVLPKI-MNALWE 425

Query: 431 LIRSLINPIFKGFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSKDD 467
           LIRS + P +KG ++ EG+     R+  L+ PG+ AH    YIN T  G +  S  D
Sbjct: 426 LIRSFVMPYWKGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLGDLPDSHLD 467

BLAST of Spg016624 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.7e-85
Identity = 187/487 (38.40%), Postives = 273/487 (56.06%), Query Frame = 0

Query: 11  DYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVIFVSVFLQKLL 70
           +Y +L P   +  DL   LF     + + +    E  E+    F  RW+IFVS+ +QKLL
Sbjct: 6   NYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKLL 65

Query: 71  DAIANLFKLFKF--------------FRRIFTK--RGKFSGMPGKTYATEVKCR---DWR 130
             +        F              F +IF    +G+F   P KT AT        D +
Sbjct: 66  IILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFI-WPEKTSATFASINGNLDQK 125

Query: 131 IEVAVGKRKNIKYGEEDFRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKLLDCYD 190
           +E+ +G  ++IK G+E  R+   L++MAS LAYE+       +++V+ + W+M LL  Y 
Sbjct: 126 VELGLG--RSIKIGDE--RYKPLLSIMASKLAYEN----EHFIRSVLQDHWQMDLLGFY- 185

Query: 191 DFWNDFQDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSWYNIKGIGNI 250
              NDF    ST+    ++T   +PN+ +V+FRGT   N  D   D++ SW+N+  +G I
Sbjct: 186 SCPNDFDQTRSTEVIVIRDT-KDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKI 245

Query: 251 HSGFMRALGLQKATGWPKDLPPSPDNH---QFAYYTLRQQLKDIVKANDKARFIFTGHSL 310
           H GFM+ALGL K  GW +++      +   Q AYYT+ +QLK++ + N  ++FI +GHSL
Sbjct: 246 HGGFMKALGLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSL 305

Query: 311 GGALAILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFKYYRYVY 370
           GGALAILF  +L  H E ++L+RL+ VYTFGQPR+G+ D   +M+  +K +  KY RYVY
Sbjct: 306 GGALAILFTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVY 365

Query: 371 SYDVVPRVPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIPWIHIVACW 430
             D+VPR+PFD   L +KHFGGC+Y +  YKG+ +E +EP+KNY   FW+IP I I A W
Sbjct: 366 CNDMVPRLPFDDKTLMFKHFGGCLYCDSFYKGK-VEEEEPNKNYFNIFWVIPKI-INAVW 425

Query: 431 ELIRSLINPIFKGFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTRYGKIQPSKDDMD 476
           ELIRS I   ++G +Y EG+     RL  L+ PG+ AH    Y+N    G   P   D  
Sbjct: 426 ELIRSFIISCWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGNFPPQVPDSQ 478

BLAST of Spg016624 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-80
Identity = 178/476 (37.39%), Postives = 262/476 (55.04%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           M  +D      YLIL+PE +  ++L   LF       R++    +  E   +SF  RW+I
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRSV----DSSETEEHSFRHRWLI 61

Query: 61  FVSVFLQKLLDAIANLFKLFKF---FRRIFTKRGKFSG--------MPGKT---YATEVK 120
           FVS+ L KLL   + L  L      F   F     FSG        MP +T   Y + + 
Sbjct: 62  FVSLVLLKLLRFFSKLLALVGSALEFSLNFLSNNSFSGLFLRGEVVMPQRTSENYQSFIG 121

Query: 121 CRDWRIEVAVGKRKNIKYGEEDFRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCWKMKL 180
             D R+ + +   +     E+  ++  +L++MAS +AYE+     + ++ VV N W MK 
Sbjct: 122 HLDTRVSLDMTLNR-----EDGEKYYAALSIMASKIAYEN----AARIKHVVENHWNMKY 181

Query: 181 LDCYDDFWNDFQDVASTQAFTFQNTAAT-----DPNVTIVAFRGTTLNNFVDIKEDINCS 240
           L    D+WN++Q+  +TQAF       T          +VAFRGT L N  D   D + +
Sbjct: 182 LGLV-DYWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDIT 241

Query: 241 WYNIKGIGNIHSGFMRALGLQKATGWPKDLPPSPDNHQ-FAYYTLRQQLKDIVKANDKAR 300
           W+ +  IGNIH GFM+ALGLQ    WPK+   +PD     AYY++R  LK ++  N   +
Sbjct: 242 WFELPNIGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTK 301

Query: 301 FIFTGHSLGGALAILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYG 360
           F+ TGHSLGGALAILF  +L  H E++LL+R+Q VYT+GQPR+G+S    FME+ ++ Y 
Sbjct: 302 FVLTGHSLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYN 361

Query: 361 FKYYRYVYSYDVVPRVPFDLWNLWYKHFGGCVYFNCCYKGEFLETDEPDKNYCLEFWLIP 420
            KYYR+VY+ D+VPR+P+D  +L +KHFG C+Y++  Y+ + +  ++ D+N+ L   +I 
Sbjct: 362 IKYYRFVYNNDIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMR-EQSDENFFLLRGIIK 421

Query: 421 WIHIVACWELIRSLINPIFKGFDYFEGFGPLLIRLFGLIFPGVSAHICPNYINTTR 457
            +   A  E IRS      KG +Y EG+     R  G+I PGVS H   +Y+N TR
Sbjct: 422 MMW-SAILEFIRSFTIVAEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATR 461

BLAST of Spg016624 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 281.2 bits (718), Expect = 1.6e-75
Identity = 160/400 (40.00%), Postives = 234/400 (58.50%), Query Frame = 0

Query: 1   MGPEDHQFSDDYLILKPESVSWFDLFLFLFPFGFVDIRALADIPEGKEDSYNSFGVRWVI 60
           M   + +F + Y ++ P   S+ DL L LF       R +   P+  +    SF  RW++
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWIL 60

Query: 61  FVSVFLQKLLDAIANLF----KLFKFFRRIFTKRGKFSG-----MPGK---------TYA 120
            +++FLQK+L  ++  F    +   ++  + T  G F       M GK         TY 
Sbjct: 61  ALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYT 120

Query: 121 TEVKCRDWRIEVAVGKRKNIKYGEEDFRFDGSLTMMASALAYEDYSPAPSVVQTVVNNCW 180
           + + C D RIE+     + I  G  +++    L++MAS ++YE    +   + +VV N W
Sbjct: 121 SFIGCSDRRIEL----DEKINVGSIEYK--SMLSIMASKISYE----SKPYITSVVKNTW 180

Query: 181 KMKLLDCYDDFWNDFQDVASTQAFTFQNTAATDPNVTIVAFRGTTLNNFVDIKEDINCSW 240
           KM L+  Y DF+N FQ+   TQAF F+ T++T+P++ +V+FRGT      D   D++ SW
Sbjct: 181 KMDLVGNY-DFYNAFQESKLTQAFVFK-TSSTNPDLIVVSFRGTEPFEAADWCTDLDLSW 240

Query: 241 YNIKGIGNIHSGFMRALGLQKATGWPKDLPPSPDNHQFAYYTLRQQLKDIVKANDKARFI 300
           Y +K +G +H+GF RALGLQK  GWPK+       HQ+AYYT+RQ L+D +  N   ++I
Sbjct: 241 YEMKNVGKVHAGFSRALGLQK-DGWPKE--NISLLHQYAYYTIRQMLRDKLGRNKNLKYI 300

Query: 301 FTGHSLGGALAILFVTILAYHGESDLLQRLQAVYTFGQPRIGNSDCANFMEQTIKTYGFK 360
            TGHSLGGALA LF  ILA HGE +LL +L+ +YTFGQPR+G+ D   FM+  +K +G +
Sbjct: 301 LTGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIE 360

Query: 361 YYRYVYSYDVVPRVPFDLWNLW-YKHFGGCVYFNCCYKGE 382
           Y R+VY+ DVVPRVPFD   L+ YKH+G C  FN  YKG+
Sbjct: 361 YERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGK 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011654114.18.2e-15962.61uncharacterized protein LOC101203879 [Cucumis sativus] >KGN55201.1 hypothetical ... [more]
XP_022135571.14.0e-15862.42uncharacterized protein LOC111007483 isoform X2 [Momordica charantia][more]
XP_022139747.13.4e-15760.45uncharacterized protein LOC111010582 [Momordica charantia][more]
XP_008452564.11.0e-15663.30PREDICTED: uncharacterized protein LOC103493550 [Cucumis melo] >KAA0064391.1 tri... [more]
XP_022135562.11.4e-15060.08uncharacterized protein LOC111007483 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
F4JFU85.8e-6733.20Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
A0A1S3ZP852.5e-6533.81Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1[more]
Q5VKJ72.0e-5137.36Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1[more]
O599522.5e-0940.43Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
P618714.0e-0732.74Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L4W44.0e-15962.61Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639890 PE... [more]
A0A6J1C1482.0e-15862.42uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1CEU21.7e-15760.45uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A5D3D9F94.8e-15763.30Triacylglycerol lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BVA94.8e-15763.30uncharacterized protein LOC103493550 OS=Cucumis melo OX=3656 GN=LOC103493550 PE=... [more]
Match NameE-valueIdentityDescription
AT1G45201.11.8e-9239.27triacylglycerol lipase-like 1 [more]
AT5G42930.17.3e-8939.41alpha/beta-Hydrolases superfamily protein [more]
AT1G56630.11.7e-8538.40alpha/beta-Hydrolases superfamily protein [more]
AT5G67050.11.6e-8037.39alpha/beta-Hydrolases superfamily protein [more]
AT1G45201.21.6e-7540.00triacylglycerol lipase-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 200..359
e-value: 1.2E-35
score: 122.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 130..409
e-value: 4.4E-46
score: 159.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 137..404
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..464
NoneNo IPR availablePANTHERPTHR46086:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..464
NoneNo IPR availableCDDcd00519Lipase_3coord: 135..381
e-value: 5.31791E-40
score: 141.845

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016624.1Spg016624.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004806 triglyceride lipase activity