Spg016586 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016586 vs. NCBI nr
Match: KAE8649841.1 (hypothetical protein Csa_012471 [Cucumis sativus])

HSP 1 Score: 1033.1 bits (2670), Expect = 1.0e-297
Identity = 576/645 (89.30%), Postives = 596/645 (92.40%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMV GP+DS   ADNSVLKSKENGDLDQLPEKNA IKFGSHEDEPVKEQAD 
Sbjct: 1   MGVEVVGFEMVNGPMDS---ADNSVLKSKENGDLDQLPEKNATIKFGSHEDEPVKEQADG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRAYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIANAAMRAKLQDSMVQK
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDN+IK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA+DLAA+KDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELASNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RDEYEKRILPSLDGRQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDEYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHK  KS  P EQEDKAE+EI GLEKLSK IPKE EVDL KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKVGKSARPLEQEDKAEDEIHGLEKLSKDIPKEPEVDLAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKAT 540
           KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYI+THIDREKKAKKKAT
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIHTHIDREKKAKKKAT 540

Query: 541 ASGS-AALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRGQ 600
            SGS A LPDEEP DA+AVEEESTE+EKVNE AEAP  VP K  I KET +R  GRQRG 
Sbjct: 541 TSGSFATLPDEEPTDADAVEEESTEVEKVNETAEAP--VPVKTKIPKETVVRRPGRQRGL 600

Query: 601 DSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
           DS+PKVI KRKK+ NY +WA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 DSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 639

BLAST of Spg016586 vs. NCBI nr
Match: TYK20260.1 (proton pump-interactor 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1020.0 bits (2636), Expect = 8.9e-294
Identity = 571/648 (88.12%), Postives = 597/648 (92.13%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMVKGP+DS   ADNSVLKSKENG+LDQLPEKNA IKFGSHEDEPVKEQ D 
Sbjct: 1   MGVEVVGFEMVKGPMDS---ADNSVLKSKENGNLDQLPEKNATIKFGSHEDEPVKEQVDG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRLYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIANAAMRAKLQDSMVQK
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDN+IK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA+DLAA+KDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELAFNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RD+YEKRILPSLDGRQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDDYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHKA KS+ P EQEDKAE+E+ GLEKLSK IPKE EVDL KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKAGKSVQPLEQEDKAEDEMHGLEKLSKDIPKEPEVDLAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTH---IDREKKAKK 540
           KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIY +T+   +DREKKAKK
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYTHTYSLTLDREKKAKK 540

Query: 541 KATASGS-AALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQ 600
           KAT SGS A LPDEEP DA+AVEEESTE+EKVNE AEAP  VP K  I KET +R  GRQ
Sbjct: 541 KATTSGSFATLPDEEPTDADAVEEESTEVEKVNETAEAP--VPVKTKIPKETIVRRPGRQ 600

Query: 601 RGQDSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
           RG DS+PKVI KRKK+ NY +WA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 RGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 642

BLAST of Spg016586 vs. NCBI nr
Match: XP_038898028.1 (proton pump-interactor 1-like [Benincasa hispida])

HSP 1 Score: 1018.8 bits (2633), Expect = 2.0e-293
Identity = 573/646 (88.70%), Postives = 592/646 (91.64%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMVKGP+DSVP ADNSVLKSKENGDLDQLPEKNA IKFGSHEDEPVKEQAD 
Sbjct: 1   MGVEVVGFEMVKGPLDSVPAADNSVLKSKENGDLDQLPEKNAPIKFGSHEDEPVKEQADG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRAYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIANAAMRAKLQDSMV K
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVHK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA++LAAKKDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARELAAKKDVKALEELAFNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RD+YEKRILPSLD RQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDDYEKRILPSLDIRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHKA K + P EQEDKAEEEIPGLEKLSK IPKE EVD  KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKAGKPIRPLEQEDKAEEEIPGLEKLSKDIPKEPEVDPAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKAT 540
           KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK         DREKKAKKKAT
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK---------DREKKAKKKAT 540

Query: 541 ASGSAALPDEEP--ADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRG 600
           ASGSA L DEEP  ADA AV+EESTELEKVNE+AEAP  VP K  I KE+G R+RGRQRG
Sbjct: 541 ASGSATLTDEEPVDADAGAVQEESTELEKVNESAEAP--VPVKTKIPKESGGRNRGRQRG 600

Query: 601 QDSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
            DS+PKVI KRKK+ NY DWA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 LDSVPKVIRKRKKSINYLDWAGPAAAGAAVLLVILLVLGYYYYYLL 634

BLAST of Spg016586 vs. NCBI nr
Match: XP_011654071.2 (proton pump-interactor 1 [Cucumis sativus])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.7e-290
Identity = 568/645 (88.06%), Postives = 587/645 (91.01%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMV GP+DS   ADNSVLKSKENGDLDQLPEKNA IKFGSHEDEPVKEQAD 
Sbjct: 1   MGVEVVGFEMVNGPMDS---ADNSVLKSKENGDLDQLPEKNATIKFGSHEDEPVKEQADG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRAYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIANAAMRAKLQDSMVQK
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDN+IK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA+DLAA+KDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELASNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RDEYEKRILPSLDGRQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDEYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHK  KS  P EQEDKAE+EI GLEKLSK IPKE EVDL KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKVGKSARPLEQEDKAEDEIHGLEKLSKDIPKEPEVDLAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKAT 540
           KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK         DREKKAKKKAT
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK---------DREKKAKKKAT 540

Query: 541 ASGS-AALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRGQ 600
            SGS A LPDEEP DA+AVEEESTE+EKVNE AEAP  VP K  I KET +R  GRQRG 
Sbjct: 541 TSGSFATLPDEEPTDADAVEEESTEVEKVNETAEAP--VPVKTKIPKETVVRRPGRQRGL 600

Query: 601 DSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
           DS+PKVI KRKK+ NY +WA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 DSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 630

BLAST of Spg016586 vs. NCBI nr
Match: XP_008452453.1 (PREDICTED: proton pump-interactor 1 isoform X1 [Cucumis melo] >XP_008452454.1 PREDICTED: proton pump-interactor 1 isoform X2 [Cucumis melo])

HSP 1 Score: 1003.8 bits (2594), Expect = 6.6e-289
Identity = 565/645 (87.60%), Postives = 588/645 (91.16%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMVKGP+DS   ADNSVLKSKENG+LDQLPEKNA IKFGSHEDEPVKEQ D 
Sbjct: 1   MGVEVVGFEMVKGPMDS---ADNSVLKSKENGNLDQLPEKNATIKFGSHEDEPVKEQVDG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRAYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQ LREIKQ EGTREKVIANAAMRAKLQDSMVQK
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQNLREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDN+IK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA+DLAA+KDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELAFNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RD+YEKRILPSLDGRQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDDYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHKA KS+ P EQEDKAE+E+ GLEKLSK IPKE EVDL KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKAGKSVQPLEQEDKAEDEMHGLEKLSKDIPKEPEVDLAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKAT 540
           KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK         DREKKAKKKAT
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK---------DREKKAKKKAT 540

Query: 541 ASGS-AALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRGQ 600
            SGS A LPDEEP DA+AVEEESTE+EKVNE AEAP  VP K  I KET +R  GRQRG 
Sbjct: 541 TSGSFATLPDEEPTDADAVEEESTEVEKVNETAEAP--VPVKTKIPKETIVRRPGRQRGL 600

Query: 601 DSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
           DS+PKVI KRKK+ NY +WA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 DSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 630

BLAST of Spg016586 vs. ExPASy Swiss-Prot
Match: O23144 (Proton pump-interactor 1 OS=Arabidopsis thaliana OX=3702 GN=PPI1 PE=1 SV=2)

HSP 1 Score: 501.5 bits (1290), Expect = 1.4e-140
Identity = 328/653 (50.23%), Postives = 437/653 (66.92%), Query Frame = 0

Query: 1   MGVEVV---GFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQ 60
           MGVEVV   GFE+   P +  P         ++NG LDQ    +A I FGS  + P   +
Sbjct: 1   MGVEVVNSGGFEVAPAPFEGKP---------EKNGKLDQGKGDDAPINFGSVGELPKNAE 60

Query: 61  ADASSTTN--IPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARF 120
            + +   N   PK+AA+EWP  KQIH+FY +++R Y DP +KAK+DLADKE++K ++AR 
Sbjct: 61  ENNNKVVNSDAPKNAAEEWPVAKQIHSFYLVKYRSYADPKIKAKLDLADKELEKLNKART 120

Query: 121 QITEALKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLR-NANNAGR 180
            + + L+AKR ER+EL   +  L+ + + F ++ DEK KE+EPL QALGKLR N   + R
Sbjct: 121 GVLDKLRAKRAERSELFDLLDPLKSERKGFNTMFDEKRKEMEPLQQALGKLRSNDGGSAR 180

Query: 181 NGGLCSSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQ 240
              +CSSEEELN++I S QYRIQHESIPL+EEKQIL+EI+  EGTR+KVIANAAMRAK++
Sbjct: 181 GPAICSSEEELNSMIYSYQYRIQHESIPLTEEKQILKEIRLLEGTRDKVIANAAMRAKIK 240

Query: 241 DSMVQKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTE 300
           +SM QK+ +Q QVK++G  LDGV+KE+QA+ A+I +L + LKA  ++I  L++EL +V+E
Sbjct: 241 ESMGQKDDIQGQVKLMGAGLDGVKKERQAISARINELSEKLKATKDEITVLENELKTVSE 300

Query: 301 KRGKAHESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALW 360
           KR KA+ +I   R+ RDE N+ +Y++R++LNKA+DLAA+K++  LE LA  EVEKF++LW
Sbjct: 301 KRDKAYSNIHDLRRQRDETNSEYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLW 360

Query: 361 NGDKAFRDEYEKRILPSLDGRQISRDGRIRNPDEKPIL----APAEPAPPQTET-----A 420
              K FR++YEKRIL SLD RQ+SRDGR+RNPDEKP++    AP++  P +TE      A
Sbjct: 361 CSKKNFREDYEKRILQSLDSRQLSRDGRMRNPDEKPLIAPEAAPSKATPSETEVVPKAKA 420

Query: 421 KPNVKRTKEEPKPVATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSI 480
           KP   + KEEP      DA  AQ  +     KA  ++      D  ++E+ GL K  K  
Sbjct: 421 KP---QPKEEPVSAPKPDATVAQNTE-----KAKDAVKVKNVADDDDDEVYGLGKPQK-- 480

Query: 481 PKETEVDLVKLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIY 540
            +E  VD    KEM++ EEIAKAK AMERKKKL EKAAAKA++RAQKEAEKK K+     
Sbjct: 481 -EEKPVDAATAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEKK----- 540

Query: 541 THIDREKKAKKKATASGSAALPDEEPADAEAVEEESTELEKVNENAEAPV--TVPAKPNI 600
              ++EKKAKKK T   +    +E P   EA EEE           EAPV    P K  +
Sbjct: 541 ---EQEKKAKKK-TGGNTETETEEVP---EASEEE----------IEAPVQEEKPQKEKV 600

Query: 601 RKETGIRSRGRQRGQDSIPKVILKRKKATNYWDWAAPAAAVLLVILLVLGYYY 637
            KE  IR+R R RG ++IP+ ILKRKK+TNYW +AAPAA V+L +LLVLGYYY
Sbjct: 601 FKEKPIRNRTRGRGPETIPRAILKRKKSTNYWVYAAPAALVVL-LLLVLGYYY 610

BLAST of Spg016586 vs. ExPASy Swiss-Prot
Match: Q762B4 (Proton pump-interactor BIP103 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP103 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.4e-123
Identity = 290/648 (44.75%), Postives = 401/648 (61.88%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MG+EVVG E        V V D  V   +EN       E+  A+ FGS         A  
Sbjct: 1   MGMEVVGTEAAPA---EVKVTDGEVNLFQENESKATAKEREEAVLFGSDNSTATANGAAN 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
            +    PKDA ++WP  ++ H+F+F++ R  +DP LK KID A+K+ QK+ QAR QI EA
Sbjct: 61  GADLAPPKDAEEDWPEARKTHSFFFVKIRLLEDPKLKMKIDQAEKDFQKKIQARSQIFEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNA--GRNGGL 180
           +KAK+ ER  +I+++K L  +N+Q+   V EK+K IEPL   LGK R+ NNA   +  G+
Sbjct: 121 IKAKKNERFGIISELKPLAAENKQYNEAVSEKLKAIEPLRNRLGKFRDENNAIRAQGAGI 180

Query: 181 CSSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMV 240
           CSS EEL   I+ L  RI HESIPL EEK+++++I++ E TR KVI+ +A RA++QD++V
Sbjct: 181 CSSIEELEKSIKRLNDRISHESIPLDEEKRLIKQIRELEKTRPKVISTSANRAQIQDTVV 240

Query: 241 QKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGK 300
           +++A+QDQVKIIG  +DGV+KE+QAVR+KIK L+D LKAID ++  LQ++LT+   ++ K
Sbjct: 241 ERDAIQDQVKIIGEGIDGVKKERQAVRSKIKVLEDELKAIDMEMGSLQEDLTAANARKDK 300

Query: 301 AHESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDK 360
           AHES+ Q R  RD  NA F+++R LL+KA+DLA++ ++  ++EL   +V+KF+A W   K
Sbjct: 301 AHESLVQLRHARDAYNASFHQNRQLLSKARDLASRSELAQVQELYKTQVDKFVAEWCNSK 360

Query: 361 AFRDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE----TAKPNVKRTKE 420
           AFR++YEKRIL SL+ RQ+SRDGR+RNPDEKPI    E A P  E     +K   K+ KE
Sbjct: 361 AFREDYEKRILSSLNSRQLSRDGRMRNPDEKPIFIETEAAAPPVEQEPIQSKMPAKQAKE 420

Query: 421 EPKPVATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLV 480
            P P A        +V    K   AKS    + +D  E E P      K  PK  EVD+ 
Sbjct: 421 APAPQAEVSPKDESRVKAIAKPSKAKS--SLDADDDYEAESP------KEKPKPKEVDVA 480

Query: 481 KLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKA 540
           KLKE+KR EE+ K +LA+ERKKKL EK AAKA+ RAQKEAEKKLK           E +A
Sbjct: 481 KLKEIKRQEEMEKNRLALERKKKLAEKQAAKAAARAQKEAEKKLKR---------EEMRA 540

Query: 541 KKKATASGSAALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGR 600
           +++A A+ + A  + +     A E ++       E+  AP + P     R+         
Sbjct: 541 RRRAGAADTEASTESDNRSDGAAEAQA-------EDDSAPASAPVMREQRESVRYSRNVV 600

Query: 601 QRGQDSIPKVILKRKKATNYWDWAAPAAAV---LLVILLVLGYYYYYL 640
            + +  +PK IL+RKKA +YW WA PAAAV   L+ +L VLGYY YYL
Sbjct: 601 TKSKAPLPKAILRRKKAQSYWSWAGPAAAVAAALVALLAVLGYYQYYL 621

BLAST of Spg016586 vs. ExPASy Swiss-Prot
Match: Q6ZBF6 (Proton pump-interactor BIP131 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP131 PE=1 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 2.2e-122
Identity = 293/648 (45.22%), Postives = 411/648 (63.43%), Query Frame = 0

Query: 3   VEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADASS 62
           +E  G E     V +V   DN + + KE+    +  E+  A  FG  E+      A +++
Sbjct: 1   MEATGAEATLSQVTAVDGEDN-LFQDKESRATAK--ERGEAAVFGL-ENIVTANGATSAA 60

Query: 63  TTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEALK 122
               PKD  DEWP PKQ HTF+F+R   Y+DP+LKAK++ ADKE QK+ QAR  I EAL+
Sbjct: 61  DLAPPKDVVDEWPEPKQTHTFFFVRICSYEDPSLKAKLEQADKECQKKIQARSHIFEALR 120

Query: 123 AKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGR--NGGLCS 182
            KR ER+ +I+++K L  +N+Q+  +V  K+KEIEPL ++LGK R+ NNA R    GLCS
Sbjct: 121 TKRSERSNIISELKPLAAENKQYNEVVSGKLKEIEPLQKSLGKFRSENNAMRAQGAGLCS 180

Query: 183 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 242
           S EEL+ +I+SL  RI HESI L EEK++++EIKQ  GTR KVI NAA RAK+QD++V++
Sbjct: 181 SIEELDQLIKSLNDRISHESISLDEEKRLVKEIKQLNGTRSKVIENAAKRAKMQDTVVER 240

Query: 243 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 302
             + DQVK IG  +D V++++QAVR KIK L+D + A+D +I  LQD+LT+ T ++ KA 
Sbjct: 241 GTIHDQVKQIGVGIDEVKRDRQAVRDKIKVLEDQIHAVDGEIAALQDDLTAATARKDKAF 300

Query: 303 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 362
           E++ + RK RD  N  F++ R++ N  +DL+A+ +V+A+++L  NEVEKFMA W   K+F
Sbjct: 301 EALNELRKTRDLNNTSFHQYRTISNSVRDLSARGEVEAVQQLCQNEVEKFMAQWCSSKSF 360

Query: 363 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTETAKP---NVKRTKEEPK 422
           R++YEKRIL SL+ RQ+SRDGR+RNPDEKPI+   + APP  +   P     K+ KE P 
Sbjct: 361 REDYEKRILVSLNSRQLSRDGRMRNPDEKPIVLETQVAPPAEQEPAPLKKPAKQAKEAPA 420

Query: 423 PVATSDALPAQKVDKEVKHKAAKSLPPSEQED--KAEEEIPGLEKLSKSIPKETEVDLVK 482
           P A  D  P  ++  +   KAAK+  P + +D     ++ P  EK       + +VD  K
Sbjct: 421 PRA--DVTPKDEIRAKAPAKAAKAKQPLDIDDIPDVHDDEPPKEK------TKPKVDEAK 480

Query: 483 LKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAK 542
           LKEMKR EEI K KLA+ERKKK  EK A KA+ RA+KEAEKKLKE         +EKKA+
Sbjct: 481 LKEMKRQEEIEKNKLALERKKKQAEKQAMKAAARAEKEAEKKLKE---------KEKKAR 540

Query: 543 KKATASGSAALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQ 602
           K++  +G A    EE A+++A   +S E E   E   APVT+  K N R  + +      
Sbjct: 541 KRSATAGGA--ESEEAAESDA---KSDEAEAQEEEPAAPVTI--KKNARHRSTV-----T 600

Query: 603 RGQDSIPKVILKRKKATNYWDWAAPAAAVLLVILLVLG----YYYYYL 640
           + +  +PK +LKRKK+  +W W AP AA+   ++ +LG    Y YYYL
Sbjct: 601 KTKTPLPKAVLKRKKSQAFWSWGAPMAALAAALVALLGALVYYQYYYL 615

BLAST of Spg016586 vs. ExPASy Swiss-Prot
Match: B3H4K7 (Proton pump-interactor 2 OS=Arabidopsis thaliana OX=3702 GN=PPI2 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.4e-71
Identity = 212/605 (35.04%), Postives = 335/605 (55.37%), Query Frame = 0

Query: 38  PEKNAAIKFGSHE-------DEPVKEQADASSTTNIPKDAADEWPAPKQIHTFYFIRHRP 97
           PE N  + FGS++            E     S  + P  A +E    ++  +FYF++   
Sbjct: 17  PEMNDLVLFGSNQSVSSCDVSTVTTEDGTVFSGDSSPGGATEEDFPEEKPFSFYFVKQPA 76

Query: 98  YDDPNLKAKIDLADKEIQKRSQARFQITEALKAKRGERAELITQMKALRDDNRQFKSIVD 157
           YDDP +KAKID A  EI + ++ R  ++ A ++++ E   L  QMK+L   +  ++ +++
Sbjct: 77  YDDPEIKAKIDEAGHEINRYNKDRIVVSNAQESEKAEILSLFGQMKSLVSKSEGYRVVIE 136

Query: 158 EKIKEIEPLNQALGKLRNANNAGRNGGLCSSEEELNAVIQSLQYRIQHESIPLSEEKQIL 217
           EK  E + L+++L  LR       +  LC S+EEL+ +I    Y+I+H SI   EE  +L
Sbjct: 137 EKKMEFDALHESLRNLR----CSTSDQLCFSKEELDHLIYIAHYQIEHGSIGFEEEDWVL 196

Query: 218 REIKQFEGTREKVIANAAMRAKLQDSMVQKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQ 277
           +E ++ +G               +DS+ +KEA  ++VK +  +L+ V+KE  A+  KI  
Sbjct: 197 KETEKADGI-----------VLSEDSLAEKEASINRVKSMAVELNEVKKELDAITWKINH 256

Query: 278 LDDALKAIDNDIKKLQDELTSVTEKRGKAHESIQQFRKNRDEGNAHFYESRSLLNKAKDL 337
           L D +    N+++ L  +   + E+R +++E I+  R  RD+G A FY+S  ++ KA++L
Sbjct: 257 LSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKAAFYQSLPVMRKAREL 316

Query: 338 AAKKDVKALEELACNEVEKFMALWNGDKAFRDEYEKRILPSLDGRQISRDGRIRNPDEKP 397
           AA  +V+ LE  A +E ++FM  WN DKAFRD+Y KRI PSL  RQ+++DGRI++P+ + 
Sbjct: 317 AASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCERQLNQDGRIKDPEVQV 376

Query: 398 ILAPAEPAPPQTETAKPNVKRTKEEPKPVATSDALPAQKVDKEVKHKAAK-SLPPSEQED 457
           +    E   P     K +    ++     +   ++   K  KE + KA   +   +E+ D
Sbjct: 377 VW---EKKVPVKGGEKVHETNREDSSSNSSQYGSVITDKRKKETRKKAMDFNRSSAEESD 436

Query: 458 KAEEEIPGLEKLSKSIPKETEVDLVKLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLR 517
             + E    EK  K   +E EVD   LKE +R E++ KA+LAMERK+KLQEKAAAKA++R
Sbjct: 437 VTDLEFSVYEKPKK---EEEEVDEETLKEREREEQLEKARLAMERKRKLQEKAAAKAAIR 496

Query: 518 AQKEAEKKLKEIIYIYTHIDREKKAKKKATASGSAALPDEEPADAEAVEEESTELEKVNE 577
           AQKEAEKKLKE          EKKAKKKA A+ S+      P++++  +E + +LEKV  
Sbjct: 497 AQKEAEKKLKEC---------EKKAKKKAAANSSS------PSESDHSQEVTKDLEKVRT 556

Query: 578 NAEAPVTVPAKPN--IRKETGIRSRGRQRGQDSIPKVILKRKKATNYWDWAAPAAAVLLV 633
            A +      K      K+   R + R RG +++PK IL R+KA  YW W   +AA+ + 
Sbjct: 557 LAVSGKEKHQKERSLFPKQRSFRYKHRGRGTEALPKAILNRRKAHKYWVWGLSSAALAVA 585

BLAST of Spg016586 vs. ExPASy Swiss-Prot
Match: P0DKC1 (Proton pump-interactor 3A OS=Arabidopsis thaliana OX=3702 GN=PPI3A PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 4.7e-64
Identity = 204/587 (34.75%), Postives = 325/587 (55.37%), Query Frame = 0

Query: 50  EDEPVKEQADASSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQK 109
           E++ +    D+SS     +D++ + P      +FY ++   YD+P +KAKID A++EI +
Sbjct: 41  EEDTIFSGGDSSSGLAAEEDSSGDKPL-----SFYIVKQPVYDNPEIKAKIDEANQEIFR 100

Query: 110 RSQARFQITEALKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNA 169
            ++ R  +  A K++  E + L TQM++L   +  ++ +++EK KE + L +AL  LR  
Sbjct: 101 CNELRINVLSAKKSELAEVSSLYTQMESLVPQSEGYRMVIEEKKKEFDTLLEALRNLR-- 160

Query: 170 NNAGRNGGLCSSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAM 229
                +  LC ++EEL+ +    QY+I++ SI L EE  +L+E ++ +G           
Sbjct: 161 --CTTSDQLCFTKEELDHLSYIAQYQIEYGSIGLEEEDWMLKETEKPDGI---------- 220

Query: 230 RAKLQDSMVQKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDEL 289
                  + +KEA  ++VK +  +L+ V+ E  A+  KI  L D L    N+I+ L  E 
Sbjct: 221 ------ILSEKEASINRVKSMALELNEVKNELDAITWKINDLSDKLWKSQNNIRVLDLEK 280

Query: 290 TSVTEKRGKAHESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEK 349
             + EKR + +  I+  R  RD+GNA F++S S++ KAK+LAA  +V+ LE  A +EV++
Sbjct: 281 AHILEKRDRFYARIKMLRIQRDKGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDR 340

Query: 350 FMALWNGDKAFRDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTETAKPNV 409
           FM LWN DKAFR++Y +RI  SL  R+++ DGRI++ D +       P      + K + 
Sbjct: 341 FMTLWNDDKAFREDYVRRISHSLCERELNEDGRIKDADLQIFWEKKVPVKTIKRSEKVH- 400

Query: 410 KRTKEEPKPVATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKET 469
           K  +E+    ++ D      V  + + K  KS            ++   EK  K   KE 
Sbjct: 401 KMNREDSSSNSSEDG----NVITDKRKKETKS------------DVIVYEKPKK---KEE 460

Query: 470 EVDLVKLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHI- 529
           E+D   LKE KR E++ KA+L MERK+KLQEKAAAKA++RAQKEAEKKLK II   +H  
Sbjct: 461 EIDEEALKERKREEQLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFF 520

Query: 530 -DREKKAKKKATASGSAALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNI--RK 589
            + EKKAKKKA A+ ++      P++++ V  +    EKV   A +      K  +   K
Sbjct: 521 NECEKKAKKKAAANSTS------PSESDQVIND----EKVRTLAVSGKEKHQKERLLFPK 572

Query: 590 ETGIRSRGRQRGQDSIPKVILKRKKATNYWDWAAPAAAVLLVILLVL 633
           +   R + R  G +++PK IL R+KA  YW W   +AA+ + ++LV+
Sbjct: 581 QRSFRYKHRGSGTEALPKAILNRRKARRYWVWGLSSAALAVSLVLVV 572

BLAST of Spg016586 vs. ExPASy TrEMBL
Match: A0A0A0L261 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637150 PE=4 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.9e-297
Identity = 575/645 (89.15%), Postives = 595/645 (92.25%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMV GP+DS   ADNSVLKSKENGDLDQLPEKNA IKFGSHEDEPVKEQAD 
Sbjct: 1   MGVEVVGFEMVNGPMDS---ADNSVLKSKENGDLDQLPEKNATIKFGSHEDEPVKEQADG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRAYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIANAAMRAKLQDSMVQK
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDN+IK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA+DLAA+KDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELASNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RDEYEKRILPSLDGRQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDEYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHK  KS  P EQEDKAE+EI GLEKLSK IPKE EVDL KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKVGKSARPLEQEDKAEDEIHGLEKLSKDIPKEPEVDLAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKAT 540
           KRAEEIAKAKLAMERKKKLQEKAA KASLRAQKEAEKKLKEIIYI+THIDREKKAKKKAT
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAEKASLRAQKEAEKKLKEIIYIHTHIDREKKAKKKAT 540

Query: 541 ASGS-AALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRGQ 600
            SGS A LPDEEP DA+AVEEESTE+EKVNE AEAP  VP K  I KET +R  GRQRG 
Sbjct: 541 TSGSFATLPDEEPTDADAVEEESTEVEKVNETAEAP--VPVKTKIPKETVVRRPGRQRGL 600

Query: 601 DSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
           DS+PKVI KRKK+ NY +WA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 DSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 639

BLAST of Spg016586 vs. ExPASy TrEMBL
Match: A0A5D3D9V7 (Proton pump-interactor 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G004000 PE=4 SV=1)

HSP 1 Score: 1020.0 bits (2636), Expect = 4.3e-294
Identity = 571/648 (88.12%), Postives = 597/648 (92.13%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMVKGP+DS   ADNSVLKSKENG+LDQLPEKNA IKFGSHEDEPVKEQ D 
Sbjct: 1   MGVEVVGFEMVKGPMDS---ADNSVLKSKENGNLDQLPEKNATIKFGSHEDEPVKEQVDG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRLYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIANAAMRAKLQDSMVQK
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDN+IK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA+DLAA+KDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELAFNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RD+YEKRILPSLDGRQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDDYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHKA KS+ P EQEDKAE+E+ GLEKLSK IPKE EVDL KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKAGKSVQPLEQEDKAEDEMHGLEKLSKDIPKEPEVDLAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTH---IDREKKAKK 540
           KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIY +T+   +DREKKAKK
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYTHTYSLTLDREKKAKK 540

Query: 541 KATASGS-AALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQ 600
           KAT SGS A LPDEEP DA+AVEEESTE+EKVNE AEAP  VP K  I KET +R  GRQ
Sbjct: 541 KATTSGSFATLPDEEPTDADAVEEESTEVEKVNETAEAP--VPVKTKIPKETIVRRPGRQ 600

Query: 601 RGQDSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
           RG DS+PKVI KRKK+ NY +WA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 RGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 642

BLAST of Spg016586 vs. ExPASy TrEMBL
Match: A0A1S3BUN5 (proton pump-interactor 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493484 PE=4 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.2e-289
Identity = 565/645 (87.60%), Postives = 588/645 (91.16%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQADA 60
           MGVEVVGFEMVKGP+DS   ADNSVLKSKENG+LDQLPEKNA IKFGSHEDEPVKEQ D 
Sbjct: 1   MGVEVVGFEMVKGPMDS---ADNSVLKSKENGNLDQLPEKNATIKFGSHEDEPVKEQVDG 60

Query: 61  SSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITEA 120
           SSTTN+PKDAADEWPAPKQIHTFYF+RHR YDDPN+KAKIDLADKEIQKRSQARFQITEA
Sbjct: 61  SSTTNVPKDAADEWPAPKQIHTFYFVRHRAYDDPNVKAKIDLADKEIQKRSQARFQITEA 120

Query: 121 LKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180
           LK KRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS
Sbjct: 121 LKGKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCS 180

Query: 181 SEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQK 240
           SEEELNAVIQSLQY IQHESIPLSEEKQ LREIKQ EGTREKVIANAAMRAKLQDSMVQK
Sbjct: 181 SEEELNAVIQSLQYHIQHESIPLSEEKQNLREIKQLEGTREKVIANAAMRAKLQDSMVQK 240

Query: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAH 300
           EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDN+IK LQDELTSVTEKRG+AH
Sbjct: 241 EALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNEIKTLQDELTSVTEKRGRAH 300

Query: 301 ESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAF 360
           ESIQQ RKNRDEGNAHFY+SRSLLNKA+DLAA+KDVKALEELA NEVEKFM+LWNGDKAF
Sbjct: 301 ESIQQLRKNRDEGNAHFYQSRSLLNKARDLAAQKDVKALEELAFNEVEKFMSLWNGDKAF 360

Query: 361 RDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKPV 420
           RD+YEKRILPSLDGRQ+SRDGRIRNPDEKPILAPAEPAPPQTE  AKPN+KRTKEEPKPV
Sbjct: 361 RDDYEKRILPSLDGRQMSRDGRIRNPDEKPILAPAEPAPPQTEMAAKPNIKRTKEEPKPV 420

Query: 421 ATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEM 480
             SD LPAQKVDKEVKHKA KS+ P EQEDKAE+E+ GLEKLSK IPKE EVDL KLKEM
Sbjct: 421 -PSDTLPAQKVDKEVKHKAGKSVQPLEQEDKAEDEMHGLEKLSKDIPKEPEVDLAKLKEM 480

Query: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKAT 540
           KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK         DREKKAKKKAT
Sbjct: 481 KRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK---------DREKKAKKKAT 540

Query: 541 ASGS-AALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRGQ 600
            SGS A LPDEEP DA+AVEEESTE+EKVNE AEAP  VP K  I KET +R  GRQRG 
Sbjct: 541 TSGSFATLPDEEPTDADAVEEESTEVEKVNETAEAP--VPVKTKIPKETIVRRPGRQRGL 600

Query: 601 DSIPKVILKRKKATNYWDWAAPA---AAVLLVILLVLGYYYYYLL 641
           DS+PKVI KRKK+ NY +WA PA   AAVLLVILLVLGYYYYYLL
Sbjct: 601 DSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILLVLGYYYYYLL 630

BLAST of Spg016586 vs. ExPASy TrEMBL
Match: A0A6J1F7E9 (proton pump-interactor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442817 PE=4 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 2.4e-281
Identity = 546/642 (85.05%), Postives = 579/642 (90.19%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQAD- 60
           MGVEVVGFEMVKGP+DS PVADN VLKS ENGDLDQLPEKN  IKFGSHE+EPVKEQAD 
Sbjct: 1   MGVEVVGFEMVKGPMDSAPVADNYVLKSDENGDLDQLPEKNVPIKFGSHEEEPVKEQADG 60

Query: 61  ASSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITE 120
           +SS +N+PKDAADEWPAPKQIH+FYFIRHRPYDDP++KAKIDLADKEI+KRSQARFQITE
Sbjct: 61  SSSASNVPKDAADEWPAPKQIHSFYFIRHRPYDDPSIKAKIDLADKEIRKRSQARFQITE 120

Query: 121 ALKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLC 180
            LK+KR ERAELITQ+K LRDDNRQ+KSIVDEKIKEIEPLNQALGK RNANNAGRNGGLC
Sbjct: 121 TLKSKRAERAELITQLKPLRDDNRQYKSIVDEKIKEIEPLNQALGKFRNANNAGRNGGLC 180

Query: 181 SSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQ 240
           SSEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIAN+AMRAKLQDSMV 
Sbjct: 181 SSEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANSAMRAKLQDSMVH 240

Query: 241 KEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKA 300
           KEALQDQVKIIGGDL+GVRKEQQAVRAKIKQL++AL+AID DIK LQDELTSVTEKRGKA
Sbjct: 241 KEALQDQVKIIGGDLNGVRKEQQAVRAKIKQLEEALEAIDEDIKTLQDELTSVTEKRGKA 300

Query: 301 HESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKA 360
           HE+IQQ RKN DEGNA FY+SRSLLNKAKDLAAKKD++ALEELA  EVEKFM+LWNGDKA
Sbjct: 301 HENIQQLRKNSDEGNAQFYQSRSLLNKAKDLAAKKDIQALEELAFTEVEKFMSLWNGDKA 360

Query: 361 FRDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKP 420
           FRD+YEKRIL SLD RQ+SRDGRIRNPDEKPILAPAEPAP QTE  AKPN+KRTKEEPKP
Sbjct: 361 FRDDYEKRILASLDIRQMSRDGRIRNPDEKPILAPAEPAPRQTEMAAKPNIKRTKEEPKP 420

Query: 421 VATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKE 480
           V  SD LPAQKVD EVK KA K + P EQEDKAEEEIP L KLSK IPKE EVDL KLKE
Sbjct: 421 V-PSDPLPAQKVDIEVKQKAGKPVRPLEQEDKAEEEIPSLLKLSKDIPKEPEVDLAKLKE 480

Query: 481 MKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKA 540
           MKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK         DREKKAKKK 
Sbjct: 481 MKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK---------DREKKAKKKV 540

Query: 541 TASGSAALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRGQ 600
           TASGSA +PDEEP +A+AVEEESTELE VNE+AEA   VP K  I KETGIRSRGRQRG 
Sbjct: 541 TASGSATVPDEEPVEADAVEEESTELENVNESAEA-APVPVKSKIPKETGIRSRGRQRGL 600

Query: 601 DSIPKVILKRKKATNYWDWAAPAAAVLLVILLVLGYYYYYLL 641
           DS+PK+I KRKK+ NYWDWAAPAAAVLLVILLVLGYYYYYLL
Sbjct: 601 DSVPKIIRKRKKSINYWDWAAPAAAVLLVILLVLGYYYYYLL 631

BLAST of Spg016586 vs. ExPASy TrEMBL
Match: A0A6J1IM70 (proton pump-interactor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476847 PE=4 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 7.9e-280
Identity = 544/642 (84.74%), Postives = 577/642 (89.88%), Query Frame = 0

Query: 1   MGVEVVGFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQAD- 60
           MGVEVVGFEMVKGP+DS PVADN VLKS ENGDLDQLPEKN  IKFGSHE+EPVKEQAD 
Sbjct: 1   MGVEVVGFEMVKGPMDSAPVADNYVLKSDENGDLDQLPEKNVPIKFGSHEEEPVKEQADG 60

Query: 61  ASSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARFQITE 120
           +SS +N+PKDAADEWPAPKQIH+FYFIRHRPYDDP++KAKIDLADKEI+KRSQARFQITE
Sbjct: 61  SSSASNVPKDAADEWPAPKQIHSFYFIRHRPYDDPSIKAKIDLADKEIRKRSQARFQITE 120

Query: 121 ALKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLC 180
            LK+KR ERAELITQ+K LRDDNRQ+KSIVDEKIKEIEPLNQALGK RNANNAGRNGGLC
Sbjct: 121 TLKSKRAERAELITQLKPLRDDNRQYKSIVDEKIKEIEPLNQALGKFRNANNAGRNGGLC 180

Query: 181 SSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQ 240
           SSEEELNAVIQSLQY IQHESIPLSEEKQILREIKQ EGTREKVIAN+AMRAKLQDSMV 
Sbjct: 181 SSEEELNAVIQSLQYHIQHESIPLSEEKQILREIKQLEGTREKVIANSAMRAKLQDSMVH 240

Query: 241 KEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKA 300
           KEALQDQVKIIGGDL+GVRKEQQAVRAKIKQL++AL+AID DIK LQDELTSVTEKRGKA
Sbjct: 241 KEALQDQVKIIGGDLNGVRKEQQAVRAKIKQLEEALEAIDEDIKTLQDELTSVTEKRGKA 300

Query: 301 HESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKA 360
           HE+IQQ RKN DEGNA FY+SRSLLNKAKDLAAKKD++ALEELA  EVEKFM+LWNGDKA
Sbjct: 301 HENIQQLRKNSDEGNAQFYQSRSLLNKAKDLAAKKDIQALEELAFTEVEKFMSLWNGDKA 360

Query: 361 FRDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTE-TAKPNVKRTKEEPKP 420
           FRD+YEKRIL SLD RQ+SRDGRIRNPDEKPILAPA+PAP QTE  AKPN+KRTKEEPKP
Sbjct: 361 FRDDYEKRILASLDIRQMSRDGRIRNPDEKPILAPADPAPRQTEMAAKPNIKRTKEEPKP 420

Query: 421 VATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKE 480
           V  SD LPAQKVD EVK KA K + P EQEDKAEEEIP L KLSK IPKE EVDL KLKE
Sbjct: 421 V-PSDPLPAQKVDIEVKQKAGKPVRPLEQEDKAEEEIPSLLKLSKDIPKEPEVDLAKLKE 480

Query: 481 MKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKA 540
           MKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK         DREKKAKKK 
Sbjct: 481 MKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLK---------DREKKAKKKV 540

Query: 541 TASGSAALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNIRKETGIRSRGRQRGQ 600
           TASGSA +PDEEP +A AVEEESTELE VNE+AEA   VP K  I KET IRSRGRQRG 
Sbjct: 541 TASGSAMVPDEEPVEAAAVEEESTELENVNESAEA-APVPVKSKIPKETSIRSRGRQRGL 600

Query: 601 DSIPKVILKRKKATNYWDWAAPAAAVLLVILLVLGYYYYYLL 641
           DS+PK+I KRKK+ NYWDWAAPAAAVLLVILLVLGYYYYYLL
Sbjct: 601 DSVPKIIRKRKKSINYWDWAAPAAAVLLVILLVLGYYYYYLL 631

BLAST of Spg016586 vs. TAIR 10
Match: AT4G27500.1 (proton pump interactor 1 )

HSP 1 Score: 501.5 bits (1290), Expect = 9.9e-142
Identity = 328/653 (50.23%), Postives = 437/653 (66.92%), Query Frame = 0

Query: 1   MGVEVV---GFEMVKGPVDSVPVADNSVLKSKENGDLDQLPEKNAAIKFGSHEDEPVKEQ 60
           MGVEVV   GFE+   P +  P         ++NG LDQ    +A I FGS  + P   +
Sbjct: 1   MGVEVVNSGGFEVAPAPFEGKP---------EKNGKLDQGKGDDAPINFGSVGELPKNAE 60

Query: 61  ADASSTTN--IPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQKRSQARF 120
            + +   N   PK+AA+EWP  KQIH+FY +++R Y DP +KAK+DLADKE++K ++AR 
Sbjct: 61  ENNNKVVNSDAPKNAAEEWPVAKQIHSFYLVKYRSYADPKIKAKLDLADKELEKLNKART 120

Query: 121 QITEALKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLR-NANNAGR 180
            + + L+AKR ER+EL   +  L+ + + F ++ DEK KE+EPL QALGKLR N   + R
Sbjct: 121 GVLDKLRAKRAERSELFDLLDPLKSERKGFNTMFDEKRKEMEPLQQALGKLRSNDGGSAR 180

Query: 181 NGGLCSSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQ 240
              +CSSEEELN++I S QYRIQHESIPL+EEKQIL+EI+  EGTR+KVIANAAMRAK++
Sbjct: 181 GPAICSSEEELNSMIYSYQYRIQHESIPLTEEKQILKEIRLLEGTRDKVIANAAMRAKIK 240

Query: 241 DSMVQKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTE 300
           +SM QK+ +Q QVK++G  LDGV+KE+QA+ A+I +L + LKA  ++I  L++EL +V+E
Sbjct: 241 ESMGQKDDIQGQVKLMGAGLDGVKKERQAISARINELSEKLKATKDEITVLENELKTVSE 300

Query: 301 KRGKAHESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALW 360
           KR KA+ +I   R+ RDE N+ +Y++R++LNKA+DLAA+K++  LE LA  EVEKF++LW
Sbjct: 301 KRDKAYSNIHDLRRQRDETNSEYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLW 360

Query: 361 NGDKAFRDEYEKRILPSLDGRQISRDGRIRNPDEKPIL----APAEPAPPQTET-----A 420
              K FR++YEKRIL SLD RQ+SRDGR+RNPDEKP++    AP++  P +TE      A
Sbjct: 361 CSKKNFREDYEKRILQSLDSRQLSRDGRMRNPDEKPLIAPEAAPSKATPSETEVVPKAKA 420

Query: 421 KPNVKRTKEEPKPVATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSI 480
           KP   + KEEP      DA  AQ  +     KA  ++      D  ++E+ GL K  K  
Sbjct: 421 KP---QPKEEPVSAPKPDATVAQNTE-----KAKDAVKVKNVADDDDDEVYGLGKPQK-- 480

Query: 481 PKETEVDLVKLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIY 540
            +E  VD    KEM++ EEIAKAK AMERKKKL EKAAAKA++RAQKEAEKK K+     
Sbjct: 481 -EEKPVDAATAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEKK----- 540

Query: 541 THIDREKKAKKKATASGSAALPDEEPADAEAVEEESTELEKVNENAEAPV--TVPAKPNI 600
              ++EKKAKKK T   +    +E P   EA EEE           EAPV    P K  +
Sbjct: 541 ---EQEKKAKKK-TGGNTETETEEVP---EASEEE----------IEAPVQEEKPQKEKV 600

Query: 601 RKETGIRSRGRQRGQDSIPKVILKRKKATNYWDWAAPAAAVLLVILLVLGYYY 637
            KE  IR+R R RG ++IP+ ILKRKK+TNYW +AAPAA V+L +LLVLGYYY
Sbjct: 601 FKEKPIRNRTRGRGPETIPRAILKRKKSTNYWVYAAPAALVVL-LLLVLGYYY 610

BLAST of Spg016586 vs. TAIR 10
Match: AT3G15340.1 (proton pump interactor 2 )

HSP 1 Score: 272.3 bits (695), Expect = 9.8e-73
Identity = 212/605 (35.04%), Postives = 335/605 (55.37%), Query Frame = 0

Query: 38  PEKNAAIKFGSHE-------DEPVKEQADASSTTNIPKDAADEWPAPKQIHTFYFIRHRP 97
           PE N  + FGS++            E     S  + P  A +E    ++  +FYF++   
Sbjct: 17  PEMNDLVLFGSNQSVSSCDVSTVTTEDGTVFSGDSSPGGATEEDFPEEKPFSFYFVKQPA 76

Query: 98  YDDPNLKAKIDLADKEIQKRSQARFQITEALKAKRGERAELITQMKALRDDNRQFKSIVD 157
           YDDP +KAKID A  EI + ++ R  ++ A ++++ E   L  QMK+L   +  ++ +++
Sbjct: 77  YDDPEIKAKIDEAGHEINRYNKDRIVVSNAQESEKAEILSLFGQMKSLVSKSEGYRVVIE 136

Query: 158 EKIKEIEPLNQALGKLRNANNAGRNGGLCSSEEELNAVIQSLQYRIQHESIPLSEEKQIL 217
           EK  E + L+++L  LR       +  LC S+EEL+ +I    Y+I+H SI   EE  +L
Sbjct: 137 EKKMEFDALHESLRNLR----CSTSDQLCFSKEELDHLIYIAHYQIEHGSIGFEEEDWVL 196

Query: 218 REIKQFEGTREKVIANAAMRAKLQDSMVQKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQ 277
           +E ++ +G               +DS+ +KEA  ++VK +  +L+ V+KE  A+  KI  
Sbjct: 197 KETEKADGI-----------VLSEDSLAEKEASINRVKSMAVELNEVKKELDAITWKINH 256

Query: 278 LDDALKAIDNDIKKLQDELTSVTEKRGKAHESIQQFRKNRDEGNAHFYESRSLLNKAKDL 337
           L D +    N+++ L  +   + E+R +++E I+  R  RD+G A FY+S  ++ KA++L
Sbjct: 257 LSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKAAFYQSLPVMRKAREL 316

Query: 338 AAKKDVKALEELACNEVEKFMALWNGDKAFRDEYEKRILPSLDGRQISRDGRIRNPDEKP 397
           AA  +V+ LE  A +E ++FM  WN DKAFRD+Y KRI PSL  RQ+++DGRI++P+ + 
Sbjct: 317 AASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCERQLNQDGRIKDPEVQV 376

Query: 398 ILAPAEPAPPQTETAKPNVKRTKEEPKPVATSDALPAQKVDKEVKHKAAK-SLPPSEQED 457
           +    E   P     K +    ++     +   ++   K  KE + KA   +   +E+ D
Sbjct: 377 VW---EKKVPVKGGEKVHETNREDSSSNSSQYGSVITDKRKKETRKKAMDFNRSSAEESD 436

Query: 458 KAEEEIPGLEKLSKSIPKETEVDLVKLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLR 517
             + E    EK  K   +E EVD   LKE +R E++ KA+LAMERK+KLQEKAAAKA++R
Sbjct: 437 VTDLEFSVYEKPKK---EEEEVDEETLKEREREEQLEKARLAMERKRKLQEKAAAKAAIR 496

Query: 518 AQKEAEKKLKEIIYIYTHIDREKKAKKKATASGSAALPDEEPADAEAVEEESTELEKVNE 577
           AQKEAEKKLKE          EKKAKKKA A+ S+      P++++  +E + +LEKV  
Sbjct: 497 AQKEAEKKLKEC---------EKKAKKKAAANSSS------PSESDHSQEVTKDLEKVRT 556

Query: 578 NAEAPVTVPAKPN--IRKETGIRSRGRQRGQDSIPKVILKRKKATNYWDWAAPAAAVLLV 633
            A +      K      K+   R + R RG +++PK IL R+KA  YW W   +AA+ + 
Sbjct: 557 LAVSGKEKHQKERSLFPKQRSFRYKHRGRGTEALPKAILNRRKAHKYWVWGLSSAALAVA 585

BLAST of Spg016586 vs. TAIR 10
Match: AT5G36780.1 (AT5G36780 and AT5G36690 represent identical copies. The duplication within clone F27C7 is believed an artifact with only one true copy existing. )

HSP 1 Score: 247.3 bits (630), Expect = 3.4e-65
Identity = 204/587 (34.75%), Postives = 325/587 (55.37%), Query Frame = 0

Query: 50  EDEPVKEQADASSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQK 109
           E++ +    D+SS     +D++ + P      +FY ++   YD+P +KAKID A++EI +
Sbjct: 41  EEDTIFSGGDSSSGLAAEEDSSGDKPL-----SFYIVKQPVYDNPEIKAKIDEANQEIFR 100

Query: 110 RSQARFQITEALKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNA 169
            ++ R  +  A K++  E + L TQM++L   +  ++ +++EK KE + L +AL  LR  
Sbjct: 101 CNELRINVLSAKKSELAEVSSLYTQMESLVPQSEGYRMVIEEKKKEFDTLLEALRNLR-- 160

Query: 170 NNAGRNGGLCSSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAM 229
                +  LC ++EEL+ +    QY+I++ SI L EE  +L+E ++ +G           
Sbjct: 161 --CTTSDQLCFTKEELDHLSYIAQYQIEYGSIGLEEEDWMLKETEKPDGI---------- 220

Query: 230 RAKLQDSMVQKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDEL 289
                  + +KEA  ++VK +  +L+ V+ E  A+  KI  L D L    N+I+ L  E 
Sbjct: 221 ------ILSEKEASINRVKSMALELNEVKNELDAITWKINDLSDKLWKSQNNIRVLDLEK 280

Query: 290 TSVTEKRGKAHESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEK 349
             + EKR + +  I+  R  RD+GNA F++S S++ KAK+LAA  +V+ LE  A +EV++
Sbjct: 281 AHILEKRDRFYARIKMLRIQRDKGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDR 340

Query: 350 FMALWNGDKAFRDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTETAKPNV 409
           FM LWN DKAFR++Y +RI  SL  R+++ DGRI++ D +       P      + K + 
Sbjct: 341 FMTLWNDDKAFREDYVRRISHSLCERELNEDGRIKDADLQIFWEKKVPVKTIKRSEKVH- 400

Query: 410 KRTKEEPKPVATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKET 469
           K  +E+    ++ D      V  + + K  KS            ++   EK  K   KE 
Sbjct: 401 KMNREDSSSNSSEDG----NVITDKRKKETKS------------DVIVYEKPKK---KEE 460

Query: 470 EVDLVKLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHI- 529
           E+D   LKE KR E++ KA+L MERK+KLQEKAAAKA++RAQKEAEKKLK II   +H  
Sbjct: 461 EIDEEALKERKREEQLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFF 520

Query: 530 -DREKKAKKKATASGSAALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNI--RK 589
            + EKKAKKKA A+ ++      P++++ V  +    EKV   A +      K  +   K
Sbjct: 521 NECEKKAKKKAAANSTS------PSESDQVIND----EKVRTLAVSGKEKHQKERLLFPK 572

Query: 590 ETGIRSRGRQRGQDSIPKVILKRKKATNYWDWAAPAAAVLLVILLVL 633
           +   R + R  G +++PK IL R+KA  YW W   +AA+ + ++LV+
Sbjct: 581 QRSFRYKHRGSGTEALPKAILNRRKARRYWVWGLSSAALAVSLVLVV 572

BLAST of Spg016586 vs. TAIR 10
Match: AT5G36690.1 (AT5G36690 and AT5G36780 represent identical copies. The duplication within clone F27C7 is believed an artifact with only one true copy existing. )

HSP 1 Score: 247.3 bits (630), Expect = 3.4e-65
Identity = 204/587 (34.75%), Postives = 325/587 (55.37%), Query Frame = 0

Query: 50  EDEPVKEQADASSTTNIPKDAADEWPAPKQIHTFYFIRHRPYDDPNLKAKIDLADKEIQK 109
           E++ +    D+SS     +D++ + P      +FY ++   YD+P +KAKID A++EI +
Sbjct: 41  EEDTIFSGGDSSSGLAAEEDSSGDKPL-----SFYIVKQPVYDNPEIKAKIDEANQEIFR 100

Query: 110 RSQARFQITEALKAKRGERAELITQMKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNA 169
            ++ R  +  A K++  E + L TQM++L   +  ++ +++EK KE + L +AL  LR  
Sbjct: 101 CNELRINVLSAKKSELAEVSSLYTQMESLVPQSEGYRMVIEEKKKEFDTLLEALRNLR-- 160

Query: 170 NNAGRNGGLCSSEEELNAVIQSLQYRIQHESIPLSEEKQILREIKQFEGTREKVIANAAM 229
                +  LC ++EEL+ +    QY+I++ SI L EE  +L+E ++ +G           
Sbjct: 161 --CTTSDQLCFTKEELDHLSYIAQYQIEYGSIGLEEEDWMLKETEKPDGI---------- 220

Query: 230 RAKLQDSMVQKEALQDQVKIIGGDLDGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDEL 289
                  + +KEA  ++VK +  +L+ V+ E  A+  KI  L D L    N+I+ L  E 
Sbjct: 221 ------ILSEKEASINRVKSMALELNEVKNELDAITWKINDLSDKLWKSQNNIRVLDLEK 280

Query: 290 TSVTEKRGKAHESIQQFRKNRDEGNAHFYESRSLLNKAKDLAAKKDVKALEELACNEVEK 349
             + EKR + +  I+  R  RD+GNA F++S S++ KAK+LAA  +V+ LE  A +EV++
Sbjct: 281 AHILEKRDRFYARIKMLRIQRDKGNAAFFQSLSVMCKAKELAASGNVRELEVFASSEVDR 340

Query: 350 FMALWNGDKAFRDEYEKRILPSLDGRQISRDGRIRNPDEKPILAPAEPAPPQTETAKPNV 409
           FM LWN DKAFR++Y +RI  SL  R+++ DGRI++ D +       P      + K + 
Sbjct: 341 FMTLWNDDKAFREDYVRRISHSLCERELNEDGRIKDADLQIFWEKKVPVKTIKRSEKVH- 400

Query: 410 KRTKEEPKPVATSDALPAQKVDKEVKHKAAKSLPPSEQEDKAEEEIPGLEKLSKSIPKET 469
           K  +E+    ++ D      V  + + K  KS            ++   EK  K   KE 
Sbjct: 401 KMNREDSSSNSSEDG----NVITDKRKKETKS------------DVIVYEKPKK---KEE 460

Query: 470 EVDLVKLKEMKRAEEIAKAKLAMERKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHI- 529
           E+D   LKE KR E++ KA+L MERK+KLQEKAAAKA++RAQKEAEKKLK II   +H  
Sbjct: 461 EIDEEALKERKREEQLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFF 520

Query: 530 -DREKKAKKKATASGSAALPDEEPADAEAVEEESTELEKVNENAEAPVTVPAKPNI--RK 589
            + EKKAKKKA A+ ++      P++++ V  +    EKV   A +      K  +   K
Sbjct: 521 NECEKKAKKKAAANSTS------PSESDQVIND----EKVRTLAVSGKEKHQKERLLFPK 572

Query: 590 ETGIRSRGRQRGQDSIPKVILKRKKATNYWDWAAPAAAVLLVILLVL 633
           +   R + R  G +++PK IL R+KA  YW W   +AA+ + ++LV+
Sbjct: 581 QRSFRYKHRGSGTEALPKAILNRRKARRYWVWGLSSAALAVSLVLVV 572

BLAST of Spg016586 vs. TAIR 10
Match: AT3G15340.2 (proton pump interactor 2 )

HSP 1 Score: 231.5 bits (589), Expect = 1.9e-60
Identity = 181/501 (36.13%), Postives = 284/501 (56.69%), Query Frame = 0

Query: 135 MKALRDDNRQFKSIVDEKIKEIEPLNQALGKLRNANNAGRNGGLCSSEEELNAVIQSLQY 194
           MK+L   +  ++ +++EK  E + L+++L  LR       +  LC S+EEL+ +I    Y
Sbjct: 1   MKSLVSKSEGYRVVIEEKKMEFDALHESLRNLR----CSTSDQLCFSKEELDHLIYIAHY 60

Query: 195 RIQHESIPLSEEKQILREIKQFEGTREKVIANAAMRAKLQDSMVQKEALQDQVKIIGGDL 254
           +I+H SI   EE  +L+E ++ +G               +DS+ +KEA  ++VK +  +L
Sbjct: 61  QIEHGSIGFEEEDWVLKETEKADGI-----------VLSEDSLAEKEASINRVKSMAVEL 120

Query: 255 DGVRKEQQAVRAKIKQLDDALKAIDNDIKKLQDELTSVTEKRGKAHESIQQFRKNRDEGN 314
           + V+KE  A+  KI  L D +    N+++ L  +   + E+R +++E I+  R  RD+G 
Sbjct: 121 NEVKKELDAITWKINHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGK 180

Query: 315 AHFYESRSLLNKAKDLAAKKDVKALEELACNEVEKFMALWNGDKAFRDEYEKRILPSLDG 374
           A FY+S  ++ KA++LAA  +V+ LE  A +E ++FM  WN DKAFRD+Y KRI PSL  
Sbjct: 181 AAFYQSLPVMRKARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCE 240

Query: 375 RQISRDGRIRNPDEKPILAPAEPAPPQTETAKPNVKRTKEEPKPVATSDALPAQKVDKEV 434
           RQ+++DGRI++P+ + +    E   P     K +    ++     +   ++   K  KE 
Sbjct: 241 RQLNQDGRIKDPEVQVVW---EKKVPVKGGEKVHETNREDSSSNSSQYGSVITDKRKKET 300

Query: 435 KHKAAK-SLPPSEQEDKAEEEIPGLEKLSKSIPKETEVDLVKLKEMKRAEEIAKAKLAME 494
           + KA   +   +E+ D  + E    EK  K   +E EVD   LKE +R E++ KA+LAME
Sbjct: 301 RKKAMDFNRSSAEESDVTDLEFSVYEKPKK---EEEEVDEETLKEREREEQLEKARLAME 360

Query: 495 RKKKLQEKAAAKASLRAQKEAEKKLKEIIYIYTHIDREKKAKKKATASGSAALPDEEPAD 554
           RK+KLQEKAAAKA++RAQKEAEKKLKE          EKKAKKKA A+ S+      P++
Sbjct: 361 RKRKLQEKAAAKAAIRAQKEAEKKLKEC---------EKKAKKKAAANSSS------PSE 420

Query: 555 AEAVEEESTELEKVNENAEAPVTVPAKPN--IRKETGIRSRGRQRGQDSIPKVILKRKKA 614
           ++  +E + +LEKV   A +      K      K+   R + R RG +++PK IL R+KA
Sbjct: 421 SDHSQEVTKDLEKVRTLAVSGKEKHQKERSLFPKQRSFRYKHRGRGTEALPKAILNRRKA 465

Query: 615 TNYWDWAAPAAAVLLVILLVL 633
             YW W   +AA+ + + LV+
Sbjct: 481 HKYWVWGLSSAALAVALFLVV 465