Spg016549 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016549 vs. NCBI nr
Match: XP_038897592.1 (activating signal cointegrator 1 complex subunit 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1503.4 bits (3891), Expect = 0.0e+00
Identity = 782/874 (89.47%), Postives = 819/874 (93.71%), Query Frame = 0

Query: 1   MSNRYNQDGNKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
           MSNRYN DGNKGLRKDQKKYIPKNQNQST ELPNPKPTLS+SLRQSLPKPSDSAA +T+A
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQNQSTNELPNPKPTLSSSLRQSLPKPSDSAAVTTSA 60

Query: 61  APSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
           APS SRIQMGANGDWVSSR SGGSFVNYLPQDEAVATGLRAE GALDPMESQRVVDLLNR
Sbjct: 61  APSTSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEAGALDPMESQRVVDLLNR 120

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
           ELSRLLKLSAKEFW EVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE+ELS
Sbjct: 121 ELSRLLKLSAKEFWREVAMDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGEYELS 180

Query: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
           RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY+HENEDLTR
Sbjct: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYSHENEDLTR 240

Query: 241 ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
           ILVENAIKSQPSIHE LPSVISHFL+IVSMMH+RC+SSLETLFSSS+HG +GYSKLQADF
Sbjct: 241 ILVENAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSNHGGSGYSKLQADF 300

Query: 301 LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
           LEVIDFINDAIVTLDSFV AYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLP L +GF
Sbjct: 301 LEVIDFINDAIVTLDSFVTAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPYLHKGF 360

Query: 361 HIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
            IV V +GDEM+ NVATSLKMLALRIVSLGWKLLEICYL DEVFG+ LPVPVSMKMFPAN
Sbjct: 361 QIVLVPQGDEMLSNVATSLKMLALRIVSLGWKLLEICYLDDEVFGHALPVPVSMKMFPAN 420

Query: 421 VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
           VEDPVIRADILIQTLREINGISQQ  DKQLGQTFLQRMEKNHS++NRINSLRNNGWI +D
Sbjct: 421 VEDPVIRADILIQTLREINGISQQASDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFVD 480

Query: 481 DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
           DEQFDYLST+VMYT TS++K+PSLSKAP MSHISEVDEDAAMLESKICQIKDLFPEYGSG
Sbjct: 481 DEQFDYLSTIVMYTPTSDVKNPSLSKAPVMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540

Query: 541 FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
           FLAACL AYNQNPEEVI+RILEGTLHSDLQSLDTSLET PVPNS ATANNRNDKGKGKL 
Sbjct: 541 FLAACLEAYNQNPEEVIERILEGTLHSDLQSLDTSLETKPVPNSRATANNRNDKGKGKLF 600

Query: 601 ESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAAL 660
           E PTVAY  QVS+ KD   EGPSVSSTS GR+VRKSKD+MPYSETLDNRNEADP+RTAAL
Sbjct: 601 EFPTVAYIDQVSRDKDLTSEGPSVSSTSVGRFVRKSKDNMPYSETLDNRNEADPVRTAAL 660

Query: 661 ISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSK 720
           ISQYEYEDEYDDSFDDLGIS+AET TEDNED + QKLSS  G+SSNSTN SSA NAP+SK
Sbjct: 661 ISQYEYEDEYDDSFDDLGISIAETTTEDNEDSIGQKLSSNSGSSSNSTNGSSASNAPNSK 720

Query: 721 WGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780
           WGSRRKPQYYVKDGKNYSYKVAGS+AV+NSDEASLVTQAQKELIYGLGRGGNLPLGAVKK
Sbjct: 721 WGSRRKPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780

Query: 781 LTESEQDSQPDVSTVDPRENG-RKSWGRGRREGGSGTAVGVPAVPEGQGKQSNVAEVSDR 840
           LTE+EQDSQPDVS  DPR+N  RKSWGRGRRE  SGTA   P +PEGQGKQ NVAEVSDR
Sbjct: 781 LTEAEQDSQPDVSAGDPRDNNVRKSWGRGRRERESGTA---PGMPEGQGKQPNVAEVSDR 840

Query: 841 GGRGGNRGRGRRGGGNHHRKDRAMNKHFAGLSGF 874
           GGRGGNRGRGRRGGG+HHRKDRAM KHFAGLSGF
Sbjct: 841 GGRGGNRGRGRRGGGDHHRKDRAMKKHFAGLSGF 871

BLAST of Spg016549 vs. NCBI nr
Match: XP_023535224.1 (activating signal cointegrator 1 complex subunit 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1500.7 bits (3884), Expect = 0.0e+00
Identity = 792/876 (90.41%), Postives = 821/876 (93.72%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKGLRK+QKKYIPKNQ QST E PNPKP+LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPSLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRIQMGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYFHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSS S+GE+GYSKLQAD
Sbjct: 241 RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSGSNGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MM +V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHSI+NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ KD  +EGPSVSST GGRYVRKSKDDMPYSETLD+RNEAD +RTAA
Sbjct: 601 FESSTVAYTDQVSQSKDLMVEGPSVSSTFGGRYVRKSKDDMPYSETLDSRNEADSVRTAA 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLSSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
           KWGS+RKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELI+GLGRGGNLPLGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAVPEGQGKQSNVAEVSD 840
           KLTESEQDSQPDVS  DPREN RKSWGRG RR+ GSG+A G   VPEGQGKQ NVAEVS+
Sbjct: 781 KLTESEQDSQPDVSAADPRENVRKSWGRGSRRDLGSGSAAG---VPEGQGKQPNVAEVSE 840

Query: 841 RGGRGGNRGRGRRG-GGNHHRKDRAMNKHFAGLSGF 874
           RGGRGGNRGRGR G  GNHHRKDRAM KHFAGLSGF
Sbjct: 841 RGGRGGNRGRGRGGASGNHHRKDRAMKKHFAGLSGF 872

BLAST of Spg016549 vs. NCBI nr
Match: KAG6591418.1 (Transcription factor basic helix-loop-helix 85, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1494.9 bits (3869), Expect = 0.0e+00
Identity = 789/876 (90.07%), Postives = 818/876 (93.38%), Query Frame = 0

Query: 1    MSNRYNQDG-NKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
            MSNRYN DG NKGLRK+QKKYIPKNQ QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 391  MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 450

Query: 61   AAPSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
            A PSMSRIQMGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 451  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 510

Query: 121  RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
            RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 511  RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 570

Query: 181  SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
            SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 571  SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 630

Query: 241  RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
            R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSSS +GE+GYSKLQAD
Sbjct: 631  RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSSRNGESGYSKLQAD 690

Query: 301  FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
             LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 691  LLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 750

Query: 361  FHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
            F IVFV RGD+MM +V TS KMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 751  FQIVFVPRGDDMMSSVVTSSKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 810

Query: 421  NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
            NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHSI+NRINSLRNNGWI +
Sbjct: 811  NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFV 870

Query: 481  DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
            DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 871  DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 930

Query: 541  GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
            GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 931  GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 990

Query: 601  IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
             ES TVAYT QVSQ KD  +EGPSVSST GGRYVRKSKDDMPYSETLD+RNEAD +RTAA
Sbjct: 991  FESSTVAYTDQVSQSKDLMVEGPSVSSTFGGRYVRKSKDDMPYSETLDSRNEADSVRTAA 1050

Query: 661  LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
            L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 1051 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLSSDLGNSSNSKNASSAQNASNS 1110

Query: 721  KWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
            KWGS+RKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELI+GLGRGGNLPLGAVK
Sbjct: 1111 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 1170

Query: 781  KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAVPEGQGKQSNVAEVSD 840
            KLT+SEQDSQPDVS  DPR+N RKSWGRG RRE GSG+A G   VPEGQGKQ NVAEVS+
Sbjct: 1171 KLTDSEQDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAG---VPEGQGKQPNVAEVSE 1230

Query: 841  RGGRGGNRGRGRRG-GGNHHRKDRAMNKHFAGLSGF 874
            RGGRGGNRGRGR G  GNHHRKDRAM KHFAGLSGF
Sbjct: 1231 RGGRGGNRGRGRGGASGNHHRKDRAMKKHFAGLSGF 1262

BLAST of Spg016549 vs. NCBI nr
Match: XP_022936905.1 (activating signal cointegrator 1 complex subunit 2-like [Cucurbita moschata])

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 788/876 (89.95%), Postives = 817/876 (93.26%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKGLRK+QKKYIPKNQ QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRIQMGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSSSS GE+GYSKLQAD
Sbjct: 241 RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSSSSGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MM +V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHS++NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+S +V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISKIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ KD  +EGPSVSST  GRYVRKSKDDMPYSETLD+RNEAD +RTA+
Sbjct: 601 FESSTVAYTDQVSQSKDLMVEGPSVSSTFSGRYVRKSKDDMPYSETLDSRNEADSVRTAS 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVSQKLSSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
           KWGS+RKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELI+GLGRGGNLPLGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAVPEGQGKQSNVAEVSD 840
           KLTESEQDSQPDVS  DPR+N RKSWGRG RRE GSG+A G   VPEGQGKQ NVAEVS+
Sbjct: 781 KLTESEQDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAG---VPEGQGKQPNVAEVSE 840

Query: 841 RGGRGGNRGRGRRG-GGNHHRKDRAMNKHFAGLSGF 874
           R GRGGNRGRGR G  GNHHRKDRAM KHFAGLSGF
Sbjct: 841 RRGRGGNRGRGRGGASGNHHRKDRAMKKHFAGLSGF 872

BLAST of Spg016549 vs. NCBI nr
Match: XP_022976975.1 (activating signal cointegrator 1 complex subunit 2-like [Cucurbita maxima])

HSP 1 Score: 1484.5 bits (3842), Expect = 0.0e+00
Identity = 783/876 (89.38%), Postives = 814/876 (92.92%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKG RK+QKKYIPKNQ QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGSNKGRRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRIQMGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSA+EFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAREFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSS S+GE+GYSKLQAD
Sbjct: 241 RSLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSGSNGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MM +V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRM+KNHSI+NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMKKNHSIMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA   NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAVINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ  D  +EGPSVSST GGRYVRKSKDDMPYSE LDNRNEAD +RTAA
Sbjct: 601 FESSTVAYTDQVSQSNDLMVEGPSVSSTFGGRYVRKSKDDMPYSEALDNRNEADSVRTAA 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKL S LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLRSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
           KWGS+RKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQ+QKELI+GLGRGGNLPLGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQSQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAVPEGQGKQSNVAEVSD 840
           KLTESE+DSQPDVS  DPR+N RKSWGRG RRE GSG+A G   VPEGQGKQ NVAEVS+
Sbjct: 781 KLTESEEDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAG---VPEGQGKQPNVAEVSE 840

Query: 841 RGGRGGNRGRGRRG-GGNHHRKDRAMNKHFAGLSGF 874
           RGGRGGNR RGR G  GNHHRKDRAM KHFAGLSGF
Sbjct: 841 RGGRGGNRVRGRGGASGNHHRKDRAMKKHFAGLSGF 872

BLAST of Spg016549 vs. ExPASy Swiss-Prot
Match: Q9H1I8 (Activating signal cointegrator 1 complex subunit 2 OS=Homo sapiens OX=9606 GN=ASCC2 PE=1 SV=3)

HSP 1 Score: 109.8 bits (273), Expect = 1.6e-22
Identity = 142/621 (22.87%), Postives = 248/621 (39.94%), Query Frame = 0

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAG--VIVGEFE 180
           +L  LL L   +FW +V  D +L + LDS+L++  R +D       G+ +   V+  +  
Sbjct: 65  DLDWLLALPHDKFWCQVIFDETLQKCLDSYLRYVPRKFD------EGVASAPEVVDMQKR 124

Query: 181 LSRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDL 240
           L R VF+   RMS++++        +S    G +L    L D+PK+LD+C ++   N  L
Sbjct: 125 LHRSVFLTFLRMSTHKE---SKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPL 184

Query: 241 TRILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQA 300
            + ++ N    QPS + +L   +   L + S + Q C    +   ++    E       +
Sbjct: 185 LQKMIGNIFTQQPSYYSDLDETLPTILQVFSNILQHCGLQGDGANTTPQKLEERGRLTPS 244

Query: 301 D-----FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLL 360
           D       +++ ++ D   TL +F+  + LA   F           D    LA  ++  +
Sbjct: 245 DMPLLELKDIVLYLCDTCTTLWAFLDIFPLACQTFQ--------KHDFCYRLASFYEAAI 304

Query: 361 PSLQQGFHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVS 420
           P ++            +     +  L  L  R+     KL+EI ++   +      +P+ 
Sbjct: 305 PEMESAI--------KKRRLEDSKLLGDLWQRLSHSRKKLMEIFHI---ILNQICLLPI- 364

Query: 421 MKMFPANVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRN 480
                  +E         I+   +I   S  + +K+    FL+  +    +   I+ L+ 
Sbjct: 365 -------LESSCDNIQGFIEEFLQI--FSSLLQEKR----FLRDYDALFPVAEDISLLQQ 424

Query: 481 NGWILIDDEQFDYLSTV------VMYTSTSNIKDPSLSKAP----------------AMS 540
              +L +      L  V      V     ++ KDPS+ + P                A S
Sbjct: 425 ASSVLDETRTAYILQAVESAWEGVDRRKATDAKDPSVIEEPNGEPNGVTVTAEAVSQASS 484

Query: 541 HISEVDEDAAM--------------LESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVI 600
           H    +E+  M              L+S I Q+KDL P+ G GF+ ACL  Y+ +PE+VI
Sbjct: 485 HPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILACLEYYHYDPEQVI 544

Query: 601 QRILEGTLHSDLQSLDTSL--ETMPVPNSSATANNRNDKGKGKLIESPTVAYTHQVSQGK 660
             ILE  L   L  LD +L  E  P P    T+ +   +     + S       +V +GK
Sbjct: 545 NNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVHKGK 604

Query: 661 DFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAALISQYEYEDEYDDSFD 697
               E  + S  +  R V   +        +       P  +    S Y YEDEYDD++D
Sbjct: 605 STRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVY-YEDEYDDTYD 640

BLAST of Spg016549 vs. ExPASy Swiss-Prot
Match: Q91WR3 (Activating signal cointegrator 1 complex subunit 2 OS=Mus musculus OX=10090 GN=Ascc2 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 6.1e-22
Identity = 146/623 (23.43%), Postives = 256/623 (41.09%), Query Frame = 0

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
           +L  LL L   +FW +V  D +L + LDS+L +  R +D  +      VA +   +  L 
Sbjct: 65  DLDWLLALPHDKFWCQVIFDETLQKCLDSYLHYVPRKFD-EWVAPTPEVADM---QNHLH 124

Query: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
           R VF+   RMS++++        +S    G +L    L D+PK+LD+C ++   N  L +
Sbjct: 125 RSVFLTFLRMSTHKE---SKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPLLQ 184

Query: 241 ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLE-TLFSSSSHGENG-YSKLQA 300
            ++ N    QPS + +L   I   L + S + Q C    + T  +    GE    +    
Sbjct: 185 KMIGNIFTQQPSYYTDLDETIPTILQVFSNILQHCGLQGDGTSTTPQKLGERSPLTPSDM 244

Query: 301 DFLEVID---FINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPS 360
             LE+ D   ++ D   TL +F+  + LA   F           D    LA  +++ +P 
Sbjct: 245 PLLELKDIVLYLCDTSTTLWAFLDIFPLACQTFQ--------KHDFCYRLASFYEMAIPE 304

Query: 361 LQQGFHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPV--- 420
           L+            +     +  L  +  R+     KL+E+ ++   +      +P+   
Sbjct: 305 LESAI--------KKRRLEDSKLLGDMWQRLSHSKKKLMEVFHI---ILNQICLLPILES 364

Query: 421 SMKMFPANVEDPVIRADILIQT---LREINGISQQVPDKQLGQTFLQRMEKNHS--IINR 480
           S       +E+ +     L+Q    LR+ +  S    D  L Q     +++  +  I+  
Sbjct: 365 SCDNIQGFIEEFLQIFSSLLQEKRFLRDYDTFSPVAEDISLLQQASSALDETRTAYILQA 424

Query: 481 INSLRNNGWILIDDEQFDYLSTVVMYTSTSN---IKDPSLSKAPAMSHISEVDED----- 540
           + S     W  +D ++   +        ++N   +    +S+ P+     E DE+     
Sbjct: 425 VES----AWEGVDRQKIKDIKDPPRAKGSNNEVTVTAEPVSEMPSQLENLEEDEECMGAA 484

Query: 541 AAM--------LESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHSDLQS 600
           AA+        L+S I Q+KDL P+ G GF+ ACL  Y+ + E+VI  ILE  L  +L  
Sbjct: 485 AALGPAVSGVELDSLISQVKDLLPDLGEGFILACLEHYSYDSEQVINNILEDRLAPELSQ 544

Query: 601 LDTSLETMPVPNSSATANNRNDKGKGKLIESPTVAYTHQVSQGKDFPI---EGPSVSSTS 660
           LD  LE    P+                  +P ++  H + Q  +F +   +   +S   
Sbjct: 545 LDRGLERQVKPD-----------------PTPLLSSRHNIFQNDEFDVFSRDSVDLSRVH 604

Query: 661 GGR----YVRKSKDD-----------MPYSETLDNRNEADPLRTAALISQYEYEDEYDDS 697
            GR     VR   +D             YS  +    E  PL+     +  +YEDEYDD+
Sbjct: 605 KGRRKEENVRSLVNDKQAVVAQWQRYQKYSVVV----EEVPLQPGEYQAD-DYEDEYDDT 633

BLAST of Spg016549 vs. ExPASy Swiss-Prot
Match: Q54VC4 (Activating signal cointegrator 1 complex subunit 2 homolog OS=Dictyostelium discoideum OX=44689 GN=ascc2 PE=3 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 2.9e-16
Identity = 151/714 (21.15%), Postives = 285/714 (39.92%), Query Frame = 0

Query: 84  SFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNRELSRLLKLSAKEFWTEVAGDTSL 143
           SF+ +LP D         E+G+     S+  +  +N +LS LLK     FW+    + SL
Sbjct: 102 SFITFLPAD-------NNEQGS-----SEESILFMNSDLSSLLKFQYNIFWSHCIFNKSL 161

Query: 144 HEFLDSFLKFRSR------------WYDFPYRGANGIVAGVIVGEFE----LSRRVFMVL 203
           +EF+DSFLKF  R              +      N I+        E    L +RVF+VL
Sbjct: 162 NEFIDSFLKFFKRDNQPIISLVNNNSVNNNNNNNNSIIKKGSKDFNESKKLLFKRVFLVL 221

Query: 204 YRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTRILVENAI 263
            RMS  ++     +  ++ + +  L+   KL  +PKL DI ++Y+    D    ++++  
Sbjct: 222 VRMSLQQE----KSGFITREFYSDLIYKNKLFTIPKLFDIVSLYSSHARDAVTTMIQSIF 281

Query: 264 KSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADFLEVI--- 323
            +QP+ +++L   + HF  I            +TLF      E   S L  + L+++   
Sbjct: 282 DTQPNYYKDL---LQHFQLI-----------SKTLF------ELNQSLLNRNLLDLLRLE 341

Query: 324 -DFINDAIVTLDSFVAAYRLAA--IFFSSAVEISCGNED----LLGTLARLHDLLLPSLQ 383
            +++ D +  L+ F+  + + +  +F    + +  G  D    +LG L   ++ ++P   
Sbjct: 342 DNYLMDIVYNLEQFIRIFPMGSHQLFDEYLMNLEKGGGDGNGGVLGWLTYFYEYIIPIFN 401

Query: 384 QGFHIVFVSRGDEMMFNVATSLKM-LALRIVSLGWKL-----------LEICYLGDEVFG 443
           +        +      N+  S+ + L   I+S+   +           L++         
Sbjct: 402 KE-----NQKASNKQVNLTPSIYVPLKQHILSIFHTIFRHHFMIKLEQLQLLIQDPCQLC 461

Query: 444 NDLPV-PVSMKMFPANVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSI 503
             LP+  +S + F            I+  T    + +  +  D     + L   E+ + +
Sbjct: 462 KKLPLDEISHRFFSLLGNITSFSQQIIGSTKSNRSKLFNEYFDNITSASILYDYEQTYKL 521

Query: 504 INRINSLRNNGWILIDDEQFDYL----------------------STVVMYTSTSNIKDP 563
            N ++ L       ID   + Y                       ST ++ ++ S+    
Sbjct: 522 SNFLDKLVTLD-SSIDFTSYTYFMQLIGKPVSQDQKQIQKPKFKKSTTIIDSNISSSSSS 581

Query: 564 SLSKAP--AMSHISEV--DEDAAMLESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQ 623
           S S +P  A + +  +  +    M   KI Q+K LFP+ G  F+  CL  YNQ+ E+VI 
Sbjct: 582 SSSSSPSNATTTVKPILSNNPVIMNTVKIDQVKSLFPDLGDWFVHCCLKYYNQDVEQVIN 641

Query: 624 RIL-EGTLHSDLQSLDTSL--------ETMPVPNSSATANNRNDKGKGKLIESPTVAYTH 683
            +  + +L   L+S+D SL        + +P P ++ T     DK       + T     
Sbjct: 642 ALCDDSSLPPHLKSMDRSLSSDPSQPSKNIPTPTTTTTTTTTTDKPNNTTTATTT----- 701

Query: 684 QVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAALISQYEYEDE 723
                        + ++TS  + + KS +++  S                +  +  Y+++
Sbjct: 702 -------------TTTTTSSSKSLEKSMNELNIS----------------IGYEKIYDEK 733

BLAST of Spg016549 vs. ExPASy TrEMBL
Match: A0A6J1FF14 (activating signal cointegrator 1 complex subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443354 PE=4 SV=1)

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 788/876 (89.95%), Postives = 817/876 (93.26%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKGLRK+QKKYIPKNQ QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRIQMGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSSSS GE+GYSKLQAD
Sbjct: 241 RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSSSSGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MM +V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRMEKNHS++NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+S +V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISKIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA A NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ KD  +EGPSVSST  GRYVRKSKDDMPYSETLD+RNEAD +RTA+
Sbjct: 601 FESSTVAYTDQVSQSKDLMVEGPSVSSTFSGRYVRKSKDDMPYSETLDSRNEADSVRTAS 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKLSS LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVSQKLSSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
           KWGS+RKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELI+GLGRGGNLPLGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAVPEGQGKQSNVAEVSD 840
           KLTESEQDSQPDVS  DPR+N RKSWGRG RRE GSG+A G   VPEGQGKQ NVAEVS+
Sbjct: 781 KLTESEQDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAG---VPEGQGKQPNVAEVSE 840

Query: 841 RGGRGGNRGRGRRG-GGNHHRKDRAMNKHFAGLSGF 874
           R GRGGNRGRGR G  GNHHRKDRAM KHFAGLSGF
Sbjct: 841 RRGRGGNRGRGRGGASGNHHRKDRAMKKHFAGLSGF 872

BLAST of Spg016549 vs. ExPASy TrEMBL
Match: A0A6J1IKZ3 (activating signal cointegrator 1 complex subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111477182 PE=4 SV=1)

HSP 1 Score: 1484.5 bits (3842), Expect = 0.0e+00
Identity = 783/876 (89.38%), Postives = 814/876 (92.92%), Query Frame = 0

Query: 1   MSNRYNQDG-NKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTT 60
           MSNRYN DG NKG RK+QKKYIPKNQ QST E PNPKP LSTSLRQSLPKPSDSAA S++
Sbjct: 1   MSNRYNHDGSNKGRRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120
           A PSMSRIQMGANGDWVSSR +GGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFEL 180
           RELSRLLKLSA+EFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAGVIVGE EL
Sbjct: 121 RELSRLLKLSAREFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLT 240
           SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQAD 300
           R LVENAIKSQPSIHE LPSVISHFL+IVSMMHQRCNSSLETLFSS S+GE+GYSKLQAD
Sbjct: 241 RSLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSGSNGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360
           FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           F IVFV RGD+MM +V TSLKMLALR+VSLGWKLLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILI 480
           NVEDPVIRADI IQTLREINGISQQ PDKQLGQTFLQRM+KNHSI+NRINSLRNNGWI +
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMKKNHSIMNRINSLRNNGWIFV 480

Query: 481 DDEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQFDY+ST+V YT T NIKD SLSKAP MSHISEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISTIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNSSA   NRNDKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAVINRNDKGKGKL 600

Query: 601 IESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVSQ  D  +EGPSVSST GGRYVRKSKDDMPYSE LDNRNEAD +RTAA
Sbjct: 601 FESSTVAYTDQVSQSNDLMVEGPSVSSTFGGRYVRKSKDDMPYSEALDNRNEADSVRTAA 660

Query: 661 LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
           L+SQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QKL S LGNSSNS NASSAQNA +S
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKLRSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
           KWGS+RKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQ+QKELI+GLGRGGNLPLGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQSQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESEQDSQPDVSTVDPRENGRKSWGRG-RREGGSGTAVGVPAVPEGQGKQSNVAEVSD 840
           KLTESE+DSQPDVS  DPR+N RKSWGRG RRE GSG+A G   VPEGQGKQ NVAEVS+
Sbjct: 781 KLTESEEDSQPDVSAADPRDNVRKSWGRGSRREVGSGSAAG---VPEGQGKQPNVAEVSE 840

Query: 841 RGGRGGNRGRGRRG-GGNHHRKDRAMNKHFAGLSGF 874
           RGGRGGNR RGR G  GNHHRKDRAM KHFAGLSGF
Sbjct: 841 RGGRGGNRVRGRGGASGNHHRKDRAMKKHFAGLSGF 872

BLAST of Spg016549 vs. ExPASy TrEMBL
Match: A0A6J1FRD2 (uncharacterized protein LOC111446220 OS=Cucurbita moschata OX=3662 GN=LOC111446220 PE=4 SV=1)

HSP 1 Score: 1476.5 bits (3821), Expect = 0.0e+00
Identity = 771/874 (88.22%), Postives = 813/874 (93.02%), Query Frame = 0

Query: 1    MSNRYNQDGNKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
            MSNRYNQDGNKGLRKDQKKYIPKNQNQS+++L NPKPTLS S R SLPK SDSAA ST A
Sbjct: 1331 MSNRYNQDGNKGLRKDQKKYIPKNQNQSSKQLLNPKPTLSASFRHSLPKSSDSAAVSTAA 1390

Query: 61   APSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
            APS+SRIQMG +GDWVSSR SGGSFVNYLPQDEAVA+GLRAEEGALDP+ESQRVVDL NR
Sbjct: 1391 APSLSRIQMGVSGDWVSSRVSGGSFVNYLPQDEAVASGLRAEEGALDPVESQRVVDLSNR 1450

Query: 121  ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
            ELSRLLKLSAKEFWTEVA DTSLHEFLDSFLKFRSRWYDFP+RGANGIVAG+I+GEFEL 
Sbjct: 1451 ELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGIIIGEFELC 1510

Query: 181  RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
            RRVFMVLYRMSSNRDPGARAADSLS+KDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR
Sbjct: 1511 RRVFMVLYRMSSNRDPGARAADSLSIKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 1570

Query: 241  ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
            ILVENAIKSQ SIHENLPSVISHFL+IVSMMHQRCNSSLETL S+SSH    +SKLQADF
Sbjct: 1571 ILVENAIKSQLSIHENLPSVISHFLSIVSMMHQRCNSSLETLISTSSH---EHSKLQADF 1630

Query: 301  LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
            LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVE+SCGNEDLLGTLARLHD+LLPSLQQGF
Sbjct: 1631 LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEVSCGNEDLLGTLARLHDILLPSLQQGF 1690

Query: 361  HIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
             IVFV + D+M+ NVA SLKMLALRIVSLGWKLLEICYLGDEVFG DLPVPVSMKMFPA+
Sbjct: 1691 QIVFVPQEDDMVSNVAKSLKMLALRIVSLGWKLLEICYLGDEVFGKDLPVPVSMKMFPAS 1750

Query: 421  VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
            VEDPVIRADILIQTLREI+GISQQVPDKQLGQTFLQ MEKNHSIINRINSLRNNGWI +D
Sbjct: 1751 VEDPVIRADILIQTLREIHGISQQVPDKQLGQTFLQHMEKNHSIINRINSLRNNGWIYVD 1810

Query: 481  DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
            DEQFDYLST+VM   TSNIKDPSLSK P M HISEVDEDAAMLESKICQIKDLFPEYG+G
Sbjct: 1811 DEQFDYLSTIVMCIPTSNIKDPSLSKTPVMRHISEVDEDAAMLESKICQIKDLFPEYGNG 1870

Query: 541  FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
            FLAACLVAYNQNPEEVIQRILEGTLH+DLQSLDTSLETMPVPNS  TAN RNDKGKGKLI
Sbjct: 1871 FLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSGTTANGRNDKGKGKLI 1930

Query: 601  ESPTVAYTHQVSQGK-DFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAA 660
            ES TVAYT QVS  K D PIEGPSVSS S GRYVRKSKD MP S+TLD+RNEADP+RTAA
Sbjct: 1931 ESSTVAYTDQVSGCKEDIPIEGPSVSSASAGRYVRKSKDGMPNSQTLDSRNEADPVRTAA 1990

Query: 661  LISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSS 720
            LISQYEYEDEYDDSFDDLGIS+AETATEDN D V +K S+ LGNSSNST A SA++AP+ 
Sbjct: 1991 LISQYEYEDEYDDSFDDLGISIAETATEDNGDFVGRKPSADLGNSSNSTTAGSARSAPNP 2050

Query: 721  KWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
            KWGSRR+PQ+YVKDGKNYSYKVAGS+AVSNS+EASLVTQAQKELIYGLGRGGNLPLGAV+
Sbjct: 2051 KWGSRRRPQFYVKDGKNYSYKVAGSVAVSNSEEASLVTQAQKELIYGLGRGGNLPLGAVR 2110

Query: 781  KLTESEQDSQPDVSTVDPRENGRKSWGRGRREGGSGTAVGVPAVPEGQGKQSNVAEVSDR 840
            KLTESEQDS PDVS VDPR+NGRKSWGRGRREGG+GTA   P+VPE QGKQ NVAEVSDR
Sbjct: 2111 KLTESEQDSPPDVSAVDPRDNGRKSWGRGRREGGNGTA---PSVPEEQGKQPNVAEVSDR 2170

Query: 841  GGRGGNRGRGRRGGGNHHRKDRAMNKHFAGLSGF 874
            GGRGGNRGRGRRGGGNHHRKDRAMNKHFAGL GF
Sbjct: 2171 GGRGGNRGRGRRGGGNHHRKDRAMNKHFAGLQGF 2198

BLAST of Spg016549 vs. ExPASy TrEMBL
Match: A0A1S4DZ72 (uncharacterized protein LOC103493893 OS=Cucumis melo OX=3656 GN=LOC103493893 PE=4 SV=1)

HSP 1 Score: 1470.7 bits (3806), Expect = 0.0e+00
Identity = 768/874 (87.87%), Postives = 806/874 (92.22%), Query Frame = 0

Query: 1    MSNRYNQDGNKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
            MSNRYN DGNKGLRKDQKKYIPKNQ QST ELPNPKPTLSTSLRQSLP PSDS     TA
Sbjct: 1385 MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDS-----TA 1444

Query: 61   APSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
            APS+SRIQMGANGDWVSSR SGGSFVNYLPQDEAVATGLRAEEGALDP+ESQRVVDLLNR
Sbjct: 1445 APSVSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNR 1504

Query: 121  ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
            ELSRLLKLSAKEFW EVA DTSLHEFLDSFLKFR+RWYDFP+RGA G VAGVIVGE+ELS
Sbjct: 1505 ELSRLLKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELS 1564

Query: 181  RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
            RRVFM LYRMSSNRDPGARAADSLSLKDHGVLLQ KKLLDLPKLLDICAIY+HENEDLTR
Sbjct: 1565 RRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTR 1624

Query: 241  ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
            ILV+NAIKSQPSIHE LPSVISHFL+IVSMMH+RC+SSLETLFSSSSHG +GY KLQADF
Sbjct: 1625 ILVDNAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADF 1684

Query: 301  LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
            LEVIDFINDAIV LDSFVAAYRLAAIFFSSAVEISCGNEDLLG LARLHDLLLPSLQQGF
Sbjct: 1685 LEVIDFINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGF 1744

Query: 361  HIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
             IV + +GDEM+ NVATSLKMLALRIVS GW LLEICYLGDEVFGNDLPVPVSMKMFPAN
Sbjct: 1745 QIVLMPQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPAN 1804

Query: 421  VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
            VEDPVIRADILIQTLREINGISQQ   KQLGQTFLQRMEKNHSI+NRINSLRNNGWI +D
Sbjct: 1805 VEDPVIRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVD 1864

Query: 481  DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
            DEQF+YLST+VMYT TS+ KDPSLSKAP +SH+SEVDEDAAMLESKICQIKDLFPEYGSG
Sbjct: 1865 DEQFNYLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSG 1924

Query: 541  FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
            FLAACLVAYNQNPEEVIQRILEGTLH DLQSLDTSLETMPVPNSSATA NR DKGKGKL 
Sbjct: 1925 FLAACLVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLF 1984

Query: 601  ESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAAL 660
            E  TV YT QVS+GKD P EGPSVSSTS GR+VRKSKDD+PYSETLDNRNEAD +RTAAL
Sbjct: 1985 EPSTVPYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAAL 2044

Query: 661  ISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSK 720
            ISQYEYEDEYDDSFDDLGIS+AETATEDNEDLV QK SS LG+SSNSTN SSAQNAP+SK
Sbjct: 2045 ISQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSK 2104

Query: 721  WGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780
            WGSRR PQYYVKDGKNYSYKVAGS+AV+NSDEASLVTQAQKELIYGLGRGGNLPLGAVKK
Sbjct: 2105 WGSRRTPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 2164

Query: 781  LTESEQDSQPDVSTVDPRENGRKSWGRGRREGGSGTAVG-VPAVPEGQGKQSNVAEVSDR 840
            LTES+QDSQP  + VDPR+N RK+WGRGRRE       G  P +PEG+GKQ NVAE SDR
Sbjct: 2165 LTESQQDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDR 2224

Query: 841  GGRGGNRGRGRRGGGNHHRKDRAMNKHFAGLSGF 874
            GGRGGNRGRGRRGGG+HHRKDRA+ KHFAGLSGF
Sbjct: 2225 GGRGGNRGRGRRGGGDHHRKDRAIRKHFAGLSGF 2253

BLAST of Spg016549 vs. ExPASy TrEMBL
Match: A0A0A0L0H3 (CUE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G642490 PE=4 SV=1)

HSP 1 Score: 1464.1 bits (3789), Expect = 0.0e+00
Identity = 766/877 (87.34%), Postives = 805/877 (91.79%), Query Frame = 0

Query: 1   MSNRYNQDGNKGLRKDQKKYIPKNQNQSTRELPNPKPTLSTSLRQSLPKPSDSAAASTTA 60
           MSNRYN DGNKGLRKDQKKYIPKNQ QST ELPNPKPTLSTSL+QSLP PSDS     TA
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTNELPNPKPTLSTSLKQSLPNPSDS-----TA 60

Query: 61  APSMSRIQMGANGDWVSSRGSGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLNR 120
           APSMSRIQMGANGDWVSSR SGGSFVNYLPQDEAVATGLRAEEGALDP+ESQRVVDLLNR
Sbjct: 61  APSMSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNR 120

Query: 121 ELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAGVIVGEFELS 180
           ELSRLLKL+AKEFW EVA DTSLHEFLDSFLKFR+RWYDFP+RGANG VAGVIVGE ELS
Sbjct: 121 ELSRLLKLNAKEFWREVAMDTSLHEFLDSFLKFRTRWYDFPHRGANGTVAGVIVGENELS 180

Query: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYNHENEDLTR 240
           RRVFM LYRMSSNRDPGARAADSLSLKDHGVLLQ KKLLDLPKLLDICAIY+HENEDLTR
Sbjct: 181 RRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTR 240

Query: 241 ILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGENGYSKLQADF 300
           ILV+NAIKSQPSIH+ LPSVISHFL IVSMMH+RC+SSLETLFSSSSHG +GYSKLQADF
Sbjct: 241 ILVDNAIKSQPSIHQTLPSVISHFLRIVSMMHERCSSSLETLFSSSSHGGSGYSKLQADF 300

Query: 301 LEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQGF 360
           LEVIDFINDAIV+LDSFV AYRLAAIFF SAVEISCGNEDLLG LARLHDLLLPSLQQGF
Sbjct: 301 LEVIDFINDAIVSLDSFVTAYRLAAIFFCSAVEISCGNEDLLGMLARLHDLLLPSLQQGF 360

Query: 361 HIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
            IV + +GDEM+ NVATSLKMLALRIVS GWKLLEICYL DEVFGNDLP+PVSMKMFPAN
Sbjct: 361 QIVLMPQGDEMISNVATSLKMLALRIVSFGWKLLEICYLDDEVFGNDLPIPVSMKMFPAN 420

Query: 421 VEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSLRNNGWILID 480
           VEDPVIRADILIQTLREINGISQQ  DKQLGQTFLQ MEKNHS +NRINSLR  GW+ +D
Sbjct: 421 VEDPVIRADILIQTLREINGISQQASDKQLGQTFLQHMEKNHSTMNRINSLRKKGWMFVD 480

Query: 481 DEQFDYLSTVVMYTSTSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540
           DEQF+YLST+VMYT TS IKDPSLSKAP +SHISEVDEDAAMLESKICQIKDLFPEYGSG
Sbjct: 481 DEQFNYLSTIVMYTPTSGIKDPSLSKAPMISHISEVDEDAAMLESKICQIKDLFPEYGSG 540

Query: 541 FLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANNRNDKGKGKLI 600
           F+AACLVAYNQNPEEVIQRILEGTLH DL SLDTSLETMPVPNSSATANNR DKGKGKL 
Sbjct: 541 FVAACLVAYNQNPEEVIQRILEGTLHVDLLSLDTSLETMPVPNSSATANNRKDKGKGKLF 600

Query: 601 ESPTVAYTHQVSQGKDFPIEGPSVSSTSGGRYVRKSKDDMPYSETLDNRNEADPLRTAAL 660
           E  TV YT QVS+GKD P EGPSVSSTS GR+VRKSKDD+PYSETLD+RNEAD +RTAAL
Sbjct: 601 EPSTVPYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDSRNEADSVRTAAL 660

Query: 661 ISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSK 720
           ISQYEYEDEYDDSFDDLGIS+AETATEDNEDLV Q+ SS L +S NSTN SSAQNAP+SK
Sbjct: 661 ISQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQRPSSHLSSSMNSTNGSSAQNAPNSK 720

Query: 721 WGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780
           WGSRR PQYYVKDGKNYSYKVAGSIAV+NSDEASLVTQAQKELIYGLGRGGNLPLGAVKK
Sbjct: 721 WGSRRTPQYYVKDGKNYSYKVAGSIAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780

Query: 781 LTESEQDSQPDVSTVDPRENGRKSWGRGR----REGGSGTAVGVPAVPEGQGKQSNVAEV 840
           LTES+QDSQPDVS VDPR+N RKSWGRGR    REGG+      P +PEG+GKQ NVAEV
Sbjct: 781 LTESQQDSQPDVSAVDPRDNVRKSWGRGRREREREGGA-----APGMPEGEGKQPNVAEV 840

Query: 841 SDRGGRGGNRGRGRRGGGNHHRKDRAMNKHFAGLSGF 874
           SDRGGRGGNRGRGRRGGG+HHRKDRAM KHFAGLSGF
Sbjct: 841 SDRGGRGGNRGRGRRGGGDHHRKDRAMKKHFAGLSGF 867

BLAST of Spg016549 vs. TAIR 10
Match: AT1G27752.1 (Ubiquitin system component Cue protein )

HSP 1 Score: 850.1 bits (2195), Expect = 1.5e-246
Identity = 487/899 (54.17%), Postives = 631/899 (70.19%), Query Frame = 0

Query: 1   MSNRYNQDGNKGLR-----KDQKKYIPKNQNQSTRELPNPKP-TLSTSLRQSLPKPSDSA 60
           MSNR +   ++  R       Q+K++PK  N +      P P +LS+SLRQS      S 
Sbjct: 1   MSNRRSNRQDENTRYVPKGHQQQKFVPKPMNPTPTSNSTPFPVSLSSSLRQS----DSSG 60

Query: 61  AASTTAAPSMSRIQMGANGDWVSSRG---SGGSFVNYLPQDEAVATGLRAEEGALDPMES 120
           A+S  +A   SR+++G  G  VSS+     GGSFVNYLPQDEAVA GL  ++G LDP+ES
Sbjct: 61  ASSRVSASGGSRVRIGDQGQLVSSKSPAQGGGSFVNYLPQDEAVAAGLGPDDGGLDPVES 120

Query: 121 QRVVDLLNRELSRLLKLSAKEFWTEVAGDTSLHEFLDSFLKFRSRWYDFPYRGANGIVAG 180
           Q VVDLLNREL+RLLKL+ ++FW EVA D SLH+FLDSFL+FRSRWYDFP+ G  GIVAG
Sbjct: 121 QGVVDLLNRELTRLLKLNPRDFWREVASDASLHDFLDSFLQFRSRWYDFPFHGVKGIVAG 180

Query: 181 VIVGEFELSRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIY 240
           VIVGE EL RRVFMVLYR+SSNRDPGA+AADSLS KDH VLLQ KKLLDLPKLLDICAIY
Sbjct: 181 VIVGELELCRRVFMVLYRISSNRDPGAKAADSLSQKDHEVLLQDKKLLDLPKLLDICAIY 240

Query: 241 NHENEDLTRILVENAIKSQPSIHENLPSVISHFLTIVSMMHQRCNSSLETLFSSSSHGEN 300
            HEN +LT+ L+ENA+KSQ  I E+L  ++SHFL I+  MH RC SSLETL SS++  ++
Sbjct: 241 GHENAELTKSLIENAVKSQNGISESLNMMLSHFLGILHTMHHRCTSSLETLVSSANSEDH 300

Query: 301 GYSKLQADFLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDL 360
           G  +L +D LEV+DFIND +V+LD+F++AY  A    +  VE S G+++LL +L RLHD 
Sbjct: 301 GRRQLHSDLLEVMDFINDGVVSLDAFISAYTPAVFILACPVETSYGSDELLRSLVRLHDS 360

Query: 361 LLPSLQQGFHIVFVSRGDEMMFNVATSLKMLALRIVSLGWKLLEICYLGDEVFGNDLPVP 420
           LLPSL +GF ++F     + + +++TSL ML+ RI SL WK+L+ICYL ++ F ++  +P
Sbjct: 361 LLPSLHRGFQVLFKDEDHDSLSDISTSLNMLSTRIGSLCWKILDICYLSNDKFNHETSIP 420

Query: 421 VSMKMFPANVEDPVIRADILIQTLREINGISQQVPDKQLGQTFLQRMEKNHSIINRINSL 480
              KMFP+ VEDP++RADILIQT REI+G+S+Q  + +     LQ++EKN+ II+R+ SL
Sbjct: 421 AVTKMFPSRVEDPMVRADILIQTFREISGLSEQSLESK--NRLLQKIEKNYRIIDRLRSL 480

Query: 481 RNNGWILIDDEQFDYLSTVVMYTS-TSNIKDPSLSKAPAMSHISEVDEDAAMLESKICQI 540
           +N GWI ++DEQ  YLS ++++++ T ++K+  L      +    +DE+A +++SKI QI
Sbjct: 481 QNAGWISMEDEQLQYLSMIMLHSADTFSMKESPLLLTDGRNAEELMDENAVVMQSKISQI 540

Query: 541 KDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHSDLQSLDTSLETMPVPNSSATANN 600
           KD+FPEYG+GFLAACL AYNQNPEEVIQRILEGTLH DLQ LDTSLETMP P S+ T  +
Sbjct: 541 KDIFPEYGNGFLAACLEAYNQNPEEVIQRILEGTLHEDLQRLDTSLETMPQPKSAPTLRS 600

Query: 601 RNDKGKGKLIESPTVAYTHQVSQGKDFPIEGPSV-----SSTSGGRYVRKSKDDMPYSET 660
           + DKGKGKLIES T +    +   K  PI  PS+     SS + GR+VRK KDD P  + 
Sbjct: 601 K-DKGKGKLIESDTSSSASAIYTEK--PITRPSLPASSASSATVGRFVRKPKDDTPSYKI 660

Query: 661 LDNRNEADPLRTAALISQYEYEDEYDDSFDDLGISVAETATEDNEDLVDQKLSSGLGNSS 720
           LD R E+D  R AAL++QYEY+DEYDDSFDDLG+S+AE+ TE+         S   GN +
Sbjct: 661 LDARKESDRERNAALLAQYEYDDEYDDSFDDLGLSIAESGTEE---------SGAFGNRA 720

Query: 721 NSTNASSAQNAPSSKWGSRRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIY 780
            S      + + + KWGSR+ PQ+YVKDGKNYSYKVAG++AV+N++EASLV +A+ + I 
Sbjct: 721 GS------EPSDAPKWGSRKNPQFYVKDGKNYSYKVAGAVAVANANEASLVNEAEGDKIL 780

Query: 781 GLGRGGNLPLGAVKKLTE------SEQDSQPDVSTVDPRENGRKSWGRGRREGGSGTAVG 840
           GLGRGGN+PLGAV+KLTE       +  S  +V+  D RENGR +W RG R  G G    
Sbjct: 781 GLGRGGNIPLGAVRKLTEYQAQRDEKGQSNVNVNPSDGRENGR-NW-RGVRGRGRGVVNR 840

Query: 841 VPAVPEGQGKQSNVAEVSDRGGRGG--NRGRGRRGGG---NHHRKDRAMNKHFAGLSGF 874
                +     +N +EV+     GG   RGRGRRGGG   NH+ KDRAM KH A +SGF
Sbjct: 841 EQPQEKSNESSNNNSEVNTEAENGGGRGRGRGRRGGGGGRNHNHKDRAMKKHIASVSGF 873

BLAST of Spg016549 vs. TAIR 10
Match: AT1G27752.2 (Ubiquitin system component Cue protein )

HSP 1 Score: 618.6 bits (1594), Expect = 7.6e-177
Identity = 352/678 (51.92%), Postives = 471/678 (69.47%), Query Frame = 0

Query: 213 LQGKKLLDLPKLLDICAIYNHENEDLTRILVENAIKSQPSIHENLPSVISHFLTIVSMMH 272
           ++ KKLLDLPKLLDICAIY HEN +LT+ L+ENA+KSQ  I E+L  ++SHFL I+  MH
Sbjct: 1   MKDKKLLDLPKLLDICAIYGHENAELTKSLIENAVKSQNGISESLNMMLSHFLGILHTMH 60

Query: 273 QRCNSSLETLFSSSSHGENGYSKLQADFLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAV 332
            RC SSLETL SS++  ++G  +L +D LEV+DFIND +V+LD+F++AY  A    +  V
Sbjct: 61  HRCTSSLETLVSSANSEDHGRRQLHSDLLEVMDFINDGVVSLDAFISAYTPAVFILACPV 120

Query: 333 EISCGNEDLLGTLARLHDLLLPSLQQGFHIVFVSRGDEMMFNVATSLKMLALRIVSLGWK 392
           E S G+++LL +L RLHD LLPSL +GF ++F     + + +++TSL ML+ RI SL WK
Sbjct: 121 ETSYGSDELLRSLVRLHDSLLPSLHRGFQVLFKDEDHDSLSDISTSLNMLSTRIGSLCWK 180

Query: 393 LLEICYLGDEVFGNDLPVPVSMKMFPANVEDPVIRADILIQTLREINGISQQVPDKQLGQ 452
           +L+ICYL ++ F ++  +P   KMFP+ VEDP++RADILIQT REI+G+S+Q  + +   
Sbjct: 181 ILDICYLSNDKFNHETSIPAVTKMFPSRVEDPMVRADILIQTFREISGLSEQSLESK--N 240

Query: 453 TFLQRMEKNHSIINRINSLRNNGWILIDDEQFDYLSTVVMYTS-TSNIKDPSLSKAPAMS 512
             LQ++EKN+ II+R+ SL+N GWI ++DEQ  YLS ++++++ T ++K+  L      +
Sbjct: 241 RLLQKIEKNYRIIDRLRSLQNAGWISMEDEQLQYLSMIMLHSADTFSMKESPLLLTDGRN 300

Query: 513 HISEVDEDAAMLESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHSDLQS 572
               +DE+A +++SKI QIKD+FPEYG+GFLAACL AYNQNPEEVIQRILEGTLH DLQ 
Sbjct: 301 AEELMDENAVVMQSKISQIKDIFPEYGNGFLAACLEAYNQNPEEVIQRILEGTLHEDLQR 360

Query: 573 LDTSLETMPVPNSSATANNRNDKGKGKLIESPTVAYTHQVSQGKDFPIEGPSV-----SS 632
           LDTSLETMP P S+ T  ++ DKGKGKLIES T +    +   K  PI  PS+     SS
Sbjct: 361 LDTSLETMPQPKSAPTLRSK-DKGKGKLIESDTSSSASAIYTEK--PITRPSLPASSASS 420

Query: 633 TSGGRYVRKSKDDMPYSETLDNRNEADPLRTAALISQYEYEDEYDDSFDDLGISVAETAT 692
            + GR+VRK KDD P  + LD R E+D  R AAL++QYEY+DEYDDSFDDLG+S+AE+ T
Sbjct: 421 ATVGRFVRKPKDDTPSYKILDARKESDRERNAALLAQYEYDDEYDDSFDDLGLSIAESGT 480

Query: 693 EDNEDLVDQKLSSGLGNSSNSTNASSAQNAPSSKWGSRRKPQYYVKDGKNYSYKVAGSIA 752
           E+         S   GN + S      + + + KWGSR+ PQ+YVKDGKNYSYKVAG++A
Sbjct: 481 EE---------SGAFGNRAGS------EPSDAPKWGSRKNPQFYVKDGKNYSYKVAGAVA 540

Query: 753 VSNSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTE------SEQDSQPDVSTVDPREN 812
           V+N++EASLV +A+ + I GLGRGGN+PLGAV+KLTE       +  S  +V+  D REN
Sbjct: 541 VANANEASLVNEAEGDKILGLGRGGNIPLGAVRKLTEYQAQRDEKGQSNVNVNPSDGREN 600

Query: 813 GRKSWGRGRREGGSGTAVGVPAVPEGQGKQSNVAEVSDRGGRGG--NRGRGRRGGG---N 872
           GR +W RG R  G G         +     +N +EV+     GG   RGRGRRGGG   N
Sbjct: 601 GR-NW-RGVRGRGRGVVNREQPQEKSNESSNNNSEVNTEAENGGGRGRGRGRRGGGGGRN 656

Query: 873 HHRKDRAMNKHFAGLSGF 874
           H+ KDRAM KH A +SGF
Sbjct: 661 HNHKDRAMKKHIASVSGF 656