Spg016536 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016536
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	scaffold9: 41124122 .. 41126575 (-)
RNA-Seq Expression	Spg016536
Synteny	Spg016536

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGACTCGGCACGGTCGGAGTCAACTACGGGCTGAACGGCGACAATCTTCCGGTGCCGACCGAGGTGATAAACTTGTACGGTCGATGTGGGATCGATATCGTGCGGATATTTGAGCCGAACCATGGGGTTCTTGATGCGATGCAGGGCAAGGGGAACCTAGTGCTGTGGCTGGGGACGAGAAATGAAGACATAGAGGGGTTTGCTACGAGCCCAATGGCGGCGAGTGTTTGGGTTAATGCTCACGTGGTGCCTTACTATGAGAAGGTCAACATTGCGTATATCACCGTCGGCAATGAGGTTGTCCCTGGCGATGCGGCTGCACCATTTGTCGCTAATGCCATTAAAAATATAATGCAGGCGCTTGTCACCGCGGGCGTCAAAACAGACATTAAGGTGACCTTTTAATTTAATTCAATTCTAAATTTGGTTTGTATTTATGGTTTCAGTACGACCTTCTTTTTATTGTTTTAAAAGTTTCTATTTGTTTTTTTAGTTCAACAATTTGAATTTTTGAGCTCTCGTTTGATAATCATTTGGTTAAGTTTTTTGTTTTTGAAAATTAAGCTTATATTCACATACTTTCACCCATAAATTTCTTGGTTTTAGTATCAATCTTCTATCTTGTTTTCAAAAACCAAGTCCAATTTTGGAAACAAAAAAAATAGTTTTCAAAAACTTGTTTTTGTTTTTAAGATTTGGCTAGGAATTCAAATGCAACCTTAACTAGGATGAAAGTCATGTTAGGAAATTTAGGAGAAAACAAACATAATTTTCAAAAACAAAAAACAAAAAACCAAATGGTTATCTAACAGGGCCTGAACTATGCTTATTTGGACAAAATTTTGATTTGGTTGGTGTTATTTGAATGAGGTGTTTTTAATTTTTAATGTTTAAGAATTTTAAAGCTTTAGTTAGACTTAATTTTATTTTGAACAATACTACTTGATTGTACATCGGTGTGTATGAATACTTCATTAAAATCTTGATAACTAAGGAGTGAAAAGTAGAAAACGAAACAACTTTTTTATCCGTACGTCTTAGTTGTTAACATTTTAATGGTAATATGCAGAGAGATCCCTACATGTATGTAATAAATTTATATTTTGGATCTTATGCATTATAATTTTGTATCCATTTGATTTATATATAATTTTTTTTTATTCATTTTTCTTTTTTGGTTAGATCTTACTCGTTATTACTATATTGAACGTGTGACTTTTTATTTCTTTTTTTTTTCTTTTGTTTGAGAAAGGTAAATTTTTTAAAAAAATTTTATGGTAATAAATTGATGTTTTTTGAAGTCAAAATAAGAAATAGAAATAGGATGTAATTTTTTTTTTCACCAAATAGGATGTAATTTTGAGTTAATTGAAAACAGTTTAGAGATGATTTATGAGTTTAACCTAATTGTAGGAATAGGAACTACATAATTTTTTTTTTGAAACTTTAGAGTATTTTTTTTTTCATATAGGGATTAAAATTATTTGAGAAAGGTAACATTTTTTTTAAAAAAAAAATTTATGGTAATTAATTGATGTTTTTTGAAGTCAAAATAAGAAATAGAAATAGATGTACGTGATTCTGAGCTAATTATTGAAAACAGTTTAGAGATGATTTATGAGTTAACATAATTGTAGGAACTACATAAATTTTTTTTGAAACTTTAGAGTAATTTTTTTTTTTCATATAGGAATTAAAATTATTTGTTCTTTAATTCAACAACACATATAAAGACGAGGATTCAAACTTTTGACTAGACGTTTACTCCACACTCGTACATCCATCCCATATGTCATTGATAGATTGAATTTAAACTCACAAACGACACCAAAGTTCATAGTTCCATCATAAAATCATCACTAAACTTTGTTACTCATAACACAGGTGACAACAGTGGTAGCGATGACCGCCTTGGCAGTCTCATACCCACCGTCTGCCGGAGCATTCAGCGACGCTGCCGCCGGACCACTAAAAGATATCATACAAGTGCTAGCCTCTTCCGGCGCACCGATCTTAATCAACGTGTACCCATATTTCGCATACGCATCAAACCCAGGACAGATCTCACTGGACTACGCCCTCTTCAGGTCGACCTCGCCGGTGGAGGTGGACGGGAACCTAAACTACTACAACCTGTTCGACGCGATGGTGGATTCATTCTACGCGGCGCTGGAGAAGATCGGCGGCGGCGGAGTGGGAATCGGGATTTCAGAGACTGGGTGGCCAAGCAAAGGGAACGAGCCGTTCACGAGCATTGATAACGCGGAGACGTATAACAAAAACTTTGTGAAGCATGTGACGAGTGGAATTGGAACTCCGAGAAGGCCGAATGTGAAGTATGATGGGATTTTGTTTGAGATGTTCAATGAAGATCTTAAGGCGGCTGGAGCTGAGCAGAATTTTGGCTTGTTTTATCCCGATACGCATCCGGTTTATCCCTTGTGGAACTGCTGA

mRNA sequence

GGACTCGGCACGGTCGGAGTCAACTACGGGCTGAACGGCGACAATCTTCCGGTGCCGACCGAGGTGATAAACTTGTACGGTCGATGTGGGATCGATATCGTGCGGATATTTGAGCCGAACCATGGGGTTCTTGATGCGATGCAGGGCAAGGGGAACCTAGTGCTGTGGCTGGGGACGAGAAATGAAGACATAGAGGGGTTTGCTACGAGCCCAATGGCGGCGAGTGTTTGGGTTAATGCTCACGTGGTGCCTTACTATGAGAAGGTCAACATTGCGTATATCACCGTCGGCAATGAGGTTGTCCCTGGCGATGCGGCTGCACCATTTGTCGCTAATGCCATTAAAAATATAATGCAGGCGCTTGTCACCGCGGGCGTCAAAACAGACATTAAGGTGACAACAGTGGTAGCGATGACCGCCTTGGCAGTCTCATACCCACCGTCTGCCGGAGCATTCAGCGACGCTGCCGCCGGACCACTAAAAGATATCATACAAGTGCTAGCCTCTTCCGGCGCACCGATCTTAATCAACGTGTACCCATATTTCGCATACGCATCAAACCCAGGACAGATCTCACTGGACTACGCCCTCTTCAGGTCGACCTCGCCGGTGGAGGTGGACGGGAACCTAAACTACTACAACCTGTTCGACGCGATGGTGGATTCATTCTACGCGGCGCTGGAGAAGATCGGCGGCGGCGGAGTGGGAATCGGGATTTCAGAGACTGGGTGGCCAAGCAAAGGGAACGAGCCGTTCACGAGCATTGATAACGCGGAGACGTATAACAAAAACTTTGTGAAGCATGTGACGAGTGGAATTGGAACTCCGAGAAGGCCGAATGTGAAGTATGATGGGATTTTGTTTGAGATGTTCAATGAAGATCTTAAGGCGGCTGGAGCTGAGCAGAATTTTGGCTTGTTTTATCCCGATACGCATCCGGTTTATCCCTTGTGGAACTGCTGA

Coding sequence (CDS)

GGACTCGGCACGGTCGGAGTCAACTACGGGCTGAACGGCGACAATCTTCCGGTGCCGACCGAGGTGATAAACTTGTACGGTCGATGTGGGATCGATATCGTGCGGATATTTGAGCCGAACCATGGGGTTCTTGATGCGATGCAGGGCAAGGGGAACCTAGTGCTGTGGCTGGGGACGAGAAATGAAGACATAGAGGGGTTTGCTACGAGCCCAATGGCGGCGAGTGTTTGGGTTAATGCTCACGTGGTGCCTTACTATGAGAAGGTCAACATTGCGTATATCACCGTCGGCAATGAGGTTGTCCCTGGCGATGCGGCTGCACCATTTGTCGCTAATGCCATTAAAAATATAATGCAGGCGCTTGTCACCGCGGGCGTCAAAACAGACATTAAGGTGACAACAGTGGTAGCGATGACCGCCTTGGCAGTCTCATACCCACCGTCTGCCGGAGCATTCAGCGACGCTGCCGCCGGACCACTAAAAGATATCATACAAGTGCTAGCCTCTTCCGGCGCACCGATCTTAATCAACGTGTACCCATATTTCGCATACGCATCAAACCCAGGACAGATCTCACTGGACTACGCCCTCTTCAGGTCGACCTCGCCGGTGGAGGTGGACGGGAACCTAAACTACTACAACCTGTTCGACGCGATGGTGGATTCATTCTACGCGGCGCTGGAGAAGATCGGCGGCGGCGGAGTGGGAATCGGGATTTCAGAGACTGGGTGGCCAAGCAAAGGGAACGAGCCGTTCACGAGCATTGATAACGCGGAGACGTATAACAAAAACTTTGTGAAGCATGTGACGAGTGGAATTGGAACTCCGAGAAGGCCGAATGTGAAGTATGATGGGATTTTGTTTGAGATGTTCAATGAAGATCTTAAGGCGGCTGGAGCTGAGCAGAATTTTGGCTTGTTTTATCCCGATACGCATCCGGTTTATCCCTTGTGGAACTGCTGA

Protein sequence

GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPYFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAEQNFGLFYPDTHPVYPLWNC

Homology

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BLAST of Spg016536 vs. NCBI nr
Match: XP_038896614.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 540.4 bits (1391), Expect = 1.0e-149
Identity = 259/320 (80.94%), Postives = 289/320 (90.31%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           G GTVGVNYGLNGDNLP+P++VINLYGRCGI+I+RIFEPNHGVLDA++G+ NLVLWLGTR
Sbjct: 30  GSGTVGVNYGLNGDNLPIPSDVINLYGRCGINIIRIFEPNHGVLDALKGQANLVLWLGTR 89

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDIEGFAT  +AA+ WVNA+VVPYY+ VNIAYITVGNEVVP DAAAPFVANAI NIMQA
Sbjct: 90  NEDIEGFATDQVAANAWVNANVVPYYKDVNIAYITVGNEVVPADAAAPFVANAITNIMQA 149

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV AG+++DIKVTTVVAMTAL VSYPPSAGAF+  AAG +KDI  VL SSGAPIL+NVYP
Sbjct: 150 LVNAGIQSDIKVTTVVAMTALEVSYPPSAGAFTATAAGVMKDIANVLGSSGAPILVNVYP 209

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISL+YALF S++PV VDG+L+Y+NLFDAMVDSFYAALEKI  GGVGI IS
Sbjct: 210 YFAYASNPEQISLNYALFTSSTPVVVDGDLHYFNLFDAMVDSFYAALEKIDAGGVGIAIS 269

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWP+ GNEPFTS+DNA TYNKNFVKHVTSG+GTP+RPNV YD ILFEMFNEDLKA G 
Sbjct: 270 ETGWPTDGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNVFYDAILFEMFNEDLKAPGV 329

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG FYP+ +PVYP WNC
Sbjct: 330 EQNFGFFYPNMNPVYPFWNC 349

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BLAST of Spg016536 vs. NCBI nr
Match: KAG6591759.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 540.4 bits (1391), Expect = 1.0e-149
Identity = 257/320 (80.31%), Postives = 286/320 (89.38%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           GLG VGVNYGLNGDNLPV +EV+ LYGRCGIDI+R++EPNH VLDA++G+ NLV+WLGTR
Sbjct: 26  GLGAVGVNYGLNGDNLPVASEVVELYGRCGIDILRVYEPNHSVLDALKGRRNLVVWLGTR 85

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDI+GFA   +AA+ WVNA+VVPYY+ VNIAY+TVGNEVVP DAAAPFVANAIKNIMQA
Sbjct: 86  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYVTVGNEVVPDDAAAPFVANAIKNIMQA 145

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV AGVK+DIKVTTVV M+ LAVSYPPSAGAFS  A GPLKDI QVL +SGAP+L+N YP
Sbjct: 146 LVIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSGTAIGPLKDITQVLGTSGAPLLLNAYP 205

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISLDYALFRST+PV VDGNL Y+NLFDAMVDSFY+ALEKIG  GV I IS
Sbjct: 206 YFAYASNPQQISLDYALFRSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDIS 265

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWPSKGNEPFTS+DNA TYNK FV+HV SG GTP+RPNVKYDG+LFEMFNEDLK AG 
Sbjct: 266 ETGWPSKGNEPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGV 325

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG+FYP+ HPVYPLWNC
Sbjct: 326 EQNFGMFYPNMHPVYPLWNC 345

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BLAST of Spg016536 vs. NCBI nr
Match: XP_022935980.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata])

HSP 1 Score: 538.1 bits (1385), Expect = 5.1e-149
Identity = 256/320 (80.00%), Postives = 286/320 (89.38%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           GLG VGVNYGLNGDNLPVP+EV+ LYGRCGIDI+R++EPNH VLDA++G+ NLV+WLGTR
Sbjct: 26  GLGAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWLGTR 85

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDI+GFA   +AA+ WVNA+VVPYY+ VNIAYITVGNEVVP DAAAPFVANAIKNIMQA
Sbjct: 86  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNIMQA 145

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV AGVK+DIKVTTVV M+ LAVSYPPSAGAFS  A GPLKDI QVL +SGAP+L+N YP
Sbjct: 146 LVIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLNAYP 205

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISLDYALF+ST+PV VDGNL Y+NLFDAMVDSFY+ALEKIG  GV I IS
Sbjct: 206 YFAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDIS 265

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWPSKGN+PFTS+DNA TYNK FV+HV SG GTP+RPNVKYDG+LFEMFNEDLK AG 
Sbjct: 266 ETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGV 325

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG+FYP+ H VYPLWNC
Sbjct: 326 EQNFGMFYPNMHSVYPLWNC 345

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BLAST of Spg016536 vs. NCBI nr
Match: XP_023536146.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 534.6 bits (1376), Expect = 5.7e-148
Identity = 254/319 (79.62%), Postives = 284/319 (89.03%), Query Frame = 0


Query: 2   LGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRN 61
           LG VGVNYGLNGDNLPVP+EV+ LYGRCGIDI+R+FEPNH VLDA++G+ NLV+WLGTRN
Sbjct: 27  LGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVFEPNHSVLDALKGRRNLVVWLGTRN 86

Query: 62  EDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQAL 121
           EDI+GFA   +AA+ WVNA+VVPYY+ VNIAYITVGNEVVP DAAAPFVA AIKNIMQAL
Sbjct: 87  EDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVAKAIKNIMQAL 146

Query: 122 VTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPY 181
           V AGVK+DIKVTTVV M+ +AVSY PSAGAFS  A GPLKDI QVL +SGAP+L+N YPY
Sbjct: 147 VIAGVKSDIKVTTVVPMSVMAVSYTPSAGAFSTTAIGPLKDITQVLGTSGAPLLLNAYPY 206

Query: 182 FAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISE 241
           FAYASNP QISLDYALF+ST+PV VDGNL Y+NLFDAMVDSFY+ALEKIG  GV I ISE
Sbjct: 207 FAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDISE 266

Query: 242 TGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAE 301
           TGWPSKGN+PFTS+DNA TYNK FV+HV SG GTP+RPNVKYDG+LFEMFNEDLK AG E
Sbjct: 267 TGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGVE 326

Query: 302 QNFGLFYPDTHPVYPLWNC 321
           QNFG+FYP+ HPVYPLWNC
Sbjct: 327 QNFGMFYPNMHPVYPLWNC 345

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BLAST of Spg016536 vs. NCBI nr
Match: XP_016899853.1 (PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo])

HSP 1 Score: 532.3 bits (1370), Expect = 2.8e-147
Identity = 253/320 (79.06%), Postives = 287/320 (89.69%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           G GT+GVNYGLNGDNLP+P++VINLY RCGI+IVR+FEPNHGVLDA+QGK +LVLWLGTR
Sbjct: 333 GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 392

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDIEGFAT+ +AA+ WVNA+VVPYY+ VNI+YITVGNEVVPGDAAAPFVANAIKNIMQA
Sbjct: 393 NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 452

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV  G+K+DIKVTTVVAMT L VSYPPSAGAF+D+AAG +KDI  VL SSGAPIL+NVYP
Sbjct: 453 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 512

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISL+YALF ST+PV VDG+L Y+NLFDAMVDSFYAALEKI    + IGIS
Sbjct: 513 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 572

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWP+ GNEPFTS+DNA TYNKN V HVTSG+GTP+RPN+KYD +LFEMFNEDLKA G 
Sbjct: 573 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 632

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG F+P+ +PVYP WNC
Sbjct: 633 EQNFGFFFPNMNPVYPFWNC 652

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BLAST of Spg016536 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 316.2 bits (809), Expect = 4.2e-85
Identity = 155/314 (49.36%), Postives = 213/314 (67.83%), Query Frame = 0


Query: 4   TVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNED 63
           ++GV YG+ G+NLP P+EV++LY    I  +R+++PN   L A++   N+ + L     D
Sbjct: 28  SIGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALR-NSNIQVLLDVPRSD 87

Query: 64  IEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALVT 123
           ++  A++P AA  W+  +VV Y+  V+  YI VGNE++PG   A ++  A++NI  AL +
Sbjct: 88  VQSLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSS 147

Query: 124 AGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPYFA 183
           AG++  IKV+T V    L  SYPPSAGAFS AA   L  I+Q LAS+GAP+L+NVYPYF+
Sbjct: 148 AGLQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFS 207

Query: 184 YASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISETG 243
           Y  NPGQISL YALF ++  V  DG  +Y NLFDA+VD+ +AALE++GG  V + +SE+G
Sbjct: 208 YTGNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESG 267

Query: 244 WPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAEQN 303
           WPS G     S  NA TYN+N ++HV  G GTPRRP  + +  +FEMFNE+ KA G EQN
Sbjct: 268 WPSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQN 327

Query: 304 FGLFYPDTHPVYPL 318
           FGLFYP+  PVY +
Sbjct: 328 FGLFYPNKQPVYQI 338

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BLAST of Spg016536 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.9e-77
Identity = 151/318 (47.48%), Postives = 208/318 (65.41%), Query Frame = 0


Query: 4   TVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNED 63
           ++G+NYGL GDNLP P+ VINLY   GI  +RIF+PN  VL+A++G  ++ + +G +++D
Sbjct: 33  SIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQD 92

Query: 64  IEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQAL-- 123
           +   A S  A   W  A++  Y   VNI +ITVGNEV+PG      +   +  +MQ+L  
Sbjct: 93  LAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-----IGPQVLPVMQSLTN 152

Query: 124 VTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPY 183
           +       I ++TVVAM+ L  SYPPSAG F+  A   L  ++++L+ +  PIL+N+YPY
Sbjct: 153 LVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY 212

Query: 184 FAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISE 243
           FAYAS+P  I LDYA F + S V  DG+L Y N+FDA+ D+F  A+EK G   + + +SE
Sbjct: 213 FAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSE 272

Query: 244 TGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAE 303
           TGWPS GN  FT+   A TYN+NFVKH+ SG GTP+RPN   +G LF  FNE+ K AG E
Sbjct: 273 TGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTE 332

Query: 304 QNFGLFYP-DTHPVYPLW 319
           QNFGL+ P D  P+Y L+
Sbjct: 333 QNFGLYNPSDMKPIYKLF 345

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BLAST of Spg016536 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.0e-75
Identity = 146/318 (45.91%), Postives = 206/318 (64.78%), Query Frame = 0


Query: 4   TVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNED 63
           ++G+NYGL GDNLP P++VI LY    I  +RIF+PN  VL+A++G  ++ + +G R++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 64  IEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQAL-- 123
           +   + S  A   W   ++ PY   +NIA+ITVGNEV+PG      +   +  +MQ+L  
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGP-----IGPQVLPVMQSLTN 161

Query: 124 VTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPY 183
           +       I ++TVVAM  L  SYPPSAG F+  A   L  ++++L+ + +PIL+ +YPY
Sbjct: 162 LVKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPY 221

Query: 184 FAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISE 243
           F+YAS+P  I LDYA F + + V  DG+L Y N+FDA+ D+F  A+EK G   + + +SE
Sbjct: 222 FSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSE 281

Query: 244 TGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAE 303
           TGWPS GN   T+ D A TYN+NFVKH+ SG GTP+RPN   DG LF  FNE+ K  G E
Sbjct: 282 TGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTE 341

Query: 304 QNFGLFYP-DTHPVYPLW 319
           QNFGL+ P D  P+Y L+
Sbjct: 342 QNFGLYNPNDMKPIYNLF 354

[Close]

BLAST of Spg016536 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 9.9e-71
Identity = 148/317 (46.69%), Postives = 203/317 (64.04%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           G+  +GVNYG+ G +LP P +V+ LY    I  VRIF P+  VL+A++  G L + LGT 
Sbjct: 3   GVEGIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALRNSG-LGVVLGTL 62

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           N D+   A+    A+ WV+++V P+   V+  YI  GNEV+PG++AA  V  A+KN+  A
Sbjct: 63  NSDLAPLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEAA 122

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           L  AG+   + VTT +A + L  SYPPS G FS+AA   +  I+  LASSG P+L+NVYP
Sbjct: 123 LQAAGL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVYP 182

Query: 181 YFAYASNPGQISLDYALFRSTSPVEV-DGNLNYYNLFDAMVDSFYAALEKIGGG-GVGIG 240
           YFAY+++P  + LDYAL  S++ V V D  + Y N+FDA++D+ YAA+EK GGG  + + 
Sbjct: 183 YFAYSADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLELV 242

Query: 241 ISETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAA 300
           +SETGWPS G     S++NA  Y  N V+HV    GTPRRP    +  +F MFNE+ K  
Sbjct: 243 VSETGWPSGGGGYGASVENAAAYINNLVRHVG---GTPRRPGKAVETYIFAMFNENQKPE 302

Query: 301 GAEQNFGLFYPDTHPVY 316
           G EQNFG+F PD   VY
Sbjct: 303 GVEQNFGMFQPDMSQVY 312

[Close]

BLAST of Spg016536 vs. ExPASy Swiss-Prot
Match: Q03773 (Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.3e-67
Identity = 144/314 (45.86%), Postives = 199/314 (63.38%), Query Frame = 0


Query: 6   GVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNEDIE 65
           GV YG  G+NLP P EV+ LY +  I  +RI+ P+  VL+A++G  N+ L L   N+++ 
Sbjct: 35  GVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRG-SNIELLLDIPNDNLR 94

Query: 66  GFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALVTAG 125
             A+S   A+ WV  ++  Y   V   Y++VGNEV P  + A F+  A++NI +A+  AG
Sbjct: 95  NLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQRAISNAG 154

Query: 126 VKTDIKVTTVVAMTALAVSYPPSAGAF-SDAAAGPLKDIIQVLASSGAPILINVYPYFAY 185
           +   +KV+T +   ALA S+PPS G+F SD     L  +I+ L ++ AP+++NVY YFAY
Sbjct: 155 LGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNVYSYFAY 214

Query: 186 ASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISETGW 245
            +NP  ISLDYALFRS S V  DG+L Y NLFDA VD+ YAALEK GGG + I +SE+GW
Sbjct: 215 TANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIVVSESGW 274

Query: 246 PSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAEQNF 305
           PS G    TS+DNA TYN N V++V    GTP+RP    +  +F MF+E+ K    E+ +
Sbjct: 275 PSSGGTA-TSLDNARTYNTNLVRNVKQ--GTPKRPGAPLETYVFAMFDENQKQPEFEKFW 334

Query: 306 GLFYPDT-HPVYPL 318
           GLF P T  P Y +
Sbjct: 335 GLFSPITKQPKYSI 344

[Close]

BLAST of Spg016536 vs. ExPASy TrEMBL
Match: A0A6J1FC84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.5e-149
Identity = 256/320 (80.00%), Postives = 286/320 (89.38%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           GLG VGVNYGLNGDNLPVP+EV+ LYGRCGIDI+R++EPNH VLDA++G+ NLV+WLGTR
Sbjct: 26  GLGAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWLGTR 85

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDI+GFA   +AA+ WVNA+VVPYY+ VNIAYITVGNEVVP DAAAPFVANAIKNIMQA
Sbjct: 86  NEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNIMQA 145

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV AGVK+DIKVTTVV M+ LAVSYPPSAGAFS  A GPLKDI QVL +SGAP+L+N YP
Sbjct: 146 LVIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLNAYP 205

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISLDYALF+ST+PV VDGNL Y+NLFDAMVDSFY+ALEKIG  GV I IS
Sbjct: 206 YFAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRIDIS 265

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWPSKGN+PFTS+DNA TYNK FV+HV SG GTP+RPNVKYDG+LFEMFNEDLK AG 
Sbjct: 266 ETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKTAGV 325

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG+FYP+ H VYPLWNC
Sbjct: 326 EQNFGMFYPNMHSVYPLWNC 345

[Close]

BLAST of Spg016536 vs. ExPASy TrEMBL
Match: A0A5A7V7B6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G002240 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.4e-147
Identity = 253/320 (79.06%), Postives = 287/320 (89.69%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           G GT+GVNYGLNGDNLP+P++VINLY RCGI+IVR+FEPNHGVLDA+QGK +LVLWLGTR
Sbjct: 345 GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 404

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDIEGFAT+ +AA+ WVNA+VVPYY+ VNI+YITVGNEVVPGDAAAPFVANAIKNIMQA
Sbjct: 405 NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 464

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV  G+K+DIKVTTVVAMT L VSYPPSAGAF+D+AAG +KDI  VL SSGAPIL+NVYP
Sbjct: 465 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 524

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISL+YALF ST+PV VDG+L Y+NLFDAMVDSFYAALEKI    + IGIS
Sbjct: 525 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 584

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWP+ GNEPFTS+DNA TYNKN V HVTSG+GTP+RPN+KYD +LFEMFNEDLKA G 
Sbjct: 585 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 644

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG F+P+ +PVYP WNC
Sbjct: 645 EQNFGFFFPNMNPVYPFWNC 664

[Close]

BLAST of Spg016536 vs. ExPASy TrEMBL
Match: A0A5D3D518 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001700 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.4e-147
Identity = 253/320 (79.06%), Postives = 287/320 (89.69%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           G GT+GVNYGLNGDNLP+P++VINLY RCGI+IVR+FEPNHGVLDA+QGK +LVLWLGTR
Sbjct: 25  GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 84

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDIEGFAT+ +AA+ WVNA+VVPYY+ VNI+YITVGNEVVPGDAAAPFVANAIKNIMQA
Sbjct: 85  NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 144

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV  G+K+DIKVTTVVAMT L VSYPPSAGAF+D+AAG +KDI  VL SSGAPIL+NVYP
Sbjct: 145 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 204

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISL+YALF ST+PV VDG+L Y+NLFDAMVDSFYAALEKI    + IGIS
Sbjct: 205 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 264

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWP+ GNEPFTS+DNA TYNKN V HVTSG+GTP+RPN+KYD +LFEMFNEDLKA G 
Sbjct: 265 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 324

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG F+P+ +PVYP WNC
Sbjct: 325 EQNFGFFFPNMNPVYPFWNC 344

[Close]

BLAST of Spg016536 vs. ExPASy TrEMBL
Match: A0A1S4DV56 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.4e-147
Identity = 253/320 (79.06%), Postives = 287/320 (89.69%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           G GT+GVNYGLNGDNLP+P++VINLY RCGI+IVR+FEPNHGVLDA+QGK +LVLWLGTR
Sbjct: 333 GSGTIGVNYGLNGDNLPIPSDVINLYRRCGINIVRLFEPNHGVLDALQGKKDLVLWLGTR 392

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDIEGFAT+ +AA+ WVNA+VVPYY+ VNI+YITVGNEVVPGDAAAPFVANAIKNIMQA
Sbjct: 393 NEDIEGFATNQLAANTWVNANVVPYYKDVNISYITVGNEVVPGDAAAPFVANAIKNIMQA 452

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV  G+K+DIKVTTVVAMT L VSYPPSAGAF+D+AAG +KDI  VL SSGAPIL+NVYP
Sbjct: 453 LVNIGIKSDIKVTTVVAMTGLEVSYPPSAGAFTDSAAGIMKDIANVLGSSGAPILVNVYP 512

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISL+YALF ST+PV VDG+L Y+NLFDAMVDSFYAALEKI    + IGIS
Sbjct: 513 YFAYASNPQQISLNYALFTSTTPVVVDGDLQYFNLFDAMVDSFYAALEKIDAEEIRIGIS 572

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWP+ GNEPFTS+DNA TYNKN V HVTSG+GTP+RPN+KYD +LFEMFNEDLKA G 
Sbjct: 573 ETGWPTSGNEPFTSVDNALTYNKNLVNHVTSGVGTPKRPNLKYDAVLFEMFNEDLKAPGV 632

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG F+P+ +PVYP WNC
Sbjct: 633 EQNFGFFFPNMNPVYPFWNC 652

[Close]

BLAST of Spg016536 vs. ExPASy TrEMBL
Match: A0A0A0L3A5 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.3e-147
Identity = 252/320 (78.75%), Postives = 287/320 (89.69%), Query Frame = 0


Query: 1   GLGTVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTR 60
           G GT+GVNYGLNGDNLP P++VINLYGRCGI+I+R+FEPNHGVLDA+QGK +LVLWLGTR
Sbjct: 25  GFGTLGVNYGLNGDNLPTPSDVINLYGRCGINILRLFEPNHGVLDALQGKKDLVLWLGTR 84

Query: 61  NEDIEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQA 120
           NEDI+GFAT+ +AA+ WVNA+VVPYY+ VNIAYIT+GNEVVPGDAAAPFVAN IKNIMQA
Sbjct: 85  NEDIQGFATNQLAANAWVNANVVPYYKNVNIAYITIGNEVVPGDAAAPFVANGIKNIMQA 144

Query: 121 LVTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYP 180
           LV  G+K+DIKVTTVVAMTAL VSYPPSAGAF+ +AAG +KDI  VL SSGAPIL+NVYP
Sbjct: 145 LVDVGIKSDIKVTTVVAMTALGVSYPPSAGAFTGSAAGIMKDIANVLGSSGAPILVNVYP 204

Query: 181 YFAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGIS 240
           YFAYASNP QISL+YALF S++PV VDGNL Y+NLFDAMVDSFYAALEKI  G + IGIS
Sbjct: 205 YFAYASNPQQISLNYALFTSSTPVVVDGNLQYFNLFDAMVDSFYAALEKIDAGEIRIGIS 264

Query: 241 ETGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGA 300
           ETGWP+ GNEPFTS++NA TYNKN VKHVTSG+GTP+RPN+KYD +LFEMFNEDLKA G 
Sbjct: 265 ETGWPTNGNEPFTSVENALTYNKNLVKHVTSGVGTPKRPNLKYDVVLFEMFNEDLKAPGV 324

Query: 301 EQNFGLFYPDTHPVYPLWNC 321
           EQNFG F P+ +PVYP WNC
Sbjct: 325 EQNFGFFSPNMNPVYPFWNC 344

[Close]

BLAST of Spg016536 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.2e-81
Identity = 152/314 (48.41%), Postives = 208/314 (66.24%), Query Frame = 0


Query: 6   GVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNEDIE 65
           GV YG NGDNLP P + ++LY +  +  +R++EP   ++ ++QG G LV  +G RNE I+
Sbjct: 25  GVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQGTGLLVA-IGPRNEAIK 84

Query: 66  GFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALVTAG 125
             A     A  W    + P Y+ V   +ITVGNEV+ G+    +V  A+KNI  AL   G
Sbjct: 85  TLAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGEIGR-YVPQAMKNIKAALTEIG 144

Query: 126 VKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPYFAYA 185
             + I VTTV++  ALA SYPPSAG F  A    + +I+ +L+S+ +P+++NVYPYFAYA
Sbjct: 145 -NSKIHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLMVNVYPYFAYA 204

Query: 186 SNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISETGWP 245
           S+P  +SL+YA FRSTSPV  DG   Y N+FDA +D+F  ALEKI  G V + ++ETGWP
Sbjct: 205 SDPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSVKVYVAETGWP 264

Query: 246 SKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAEQNFG 305
           ++GN+P+TS++NA  YN+  +K +T+G GTPRRPNV      FEMFNEDLK    EQ+FG
Sbjct: 265 TRGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDLKQGAVEQSFG 324

Query: 306 LFYPDTHPVYPLWN 320
            F P+  PVY +WN
Sbjct: 325 FFDPNMAPVYDMWN 334

[Close]

BLAST of Spg016536 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 290.8 bits (743), Expect = 1.3e-78
Identity = 151/318 (47.48%), Postives = 208/318 (65.41%), Query Frame = 0


Query: 4   TVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNED 63
           ++G+NYGL GDNLP P+ VINLY   GI  +RIF+PN  VL+A++G  ++ + +G +++D
Sbjct: 33  SIGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQD 92

Query: 64  IEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQAL-- 123
           +   A S  A   W  A++  Y   VNI +ITVGNEV+PG      +   +  +MQ+L  
Sbjct: 93  LAALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGP-----IGPQVLPVMQSLTN 152

Query: 124 VTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPY 183
           +       I ++TVVAM+ L  SYPPSAG F+  A   L  ++++L+ +  PIL+N+YPY
Sbjct: 153 LVKSRNLPISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY 212

Query: 184 FAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISE 243
           FAYAS+P  I LDYA F + S V  DG+L Y N+FDA+ D+F  A+EK G   + + +SE
Sbjct: 213 FAYASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSE 272

Query: 244 TGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAE 303
           TGWPS GN  FT+   A TYN+NFVKH+ SG GTP+RPN   +G LF  FNE+ K AG E
Sbjct: 273 TGWPSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTE 332

Query: 304 QNFGLFYP-DTHPVYPLW 319
           QNFGL+ P D  P+Y L+
Sbjct: 333 QNFGLYNPSDMKPIYKLF 345

[Close]

BLAST of Spg016536 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.6e-76
Identity = 152/312 (48.72%), Postives = 202/312 (64.74%), Query Frame = 0


Query: 9   YGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNEDIEGFA 68
           YG NG+NLP P + + LY    ID +R++EP   +L+A++G G LV + G RNEDI+  A
Sbjct: 34  YGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADMLEALRGSGLLVAF-GPRNEDIQSLA 93

Query: 69  TSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALVTAGVKT 128
             P AA+ +V+  + PY   V I +IT+GNEV PG+  A FVA AIKN+  AL  +GV T
Sbjct: 94  HDPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGE-IAQFVAAAIKNVNVALTNSGV-T 153

Query: 129 DIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPYFAYASNP 188
            I VTTV+AMTAL  +YPPSA  F       + +I  +L+ + +P++ N+YPYFAYAS+P
Sbjct: 154 GISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITSILSETNSPLMTNIYPYFAYASDP 213

Query: 189 GQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISETGWPSKG 248
             ISLDYA F+S +PV +DG+L Y N+F+AMVD F AALEKI    V + ++ETGWP++G
Sbjct: 214 YHISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNAALEKINAANVVVMVAETGWPTEG 273

Query: 249 NEPFTSIDNAETYNKNF--VKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAEQNFGL 308
           N P TS+DNA+ YN              TPRR N   D  LF MF E+ K    EQ+FG+
Sbjct: 274 NPPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPVDVFLFAMFKENQKDGPVEQSFGI 333

Query: 309 FYPDTHPVYPLW 319
           F PD  PVY L+
Sbjct: 334 FAPDMTPVYDLF 342

[Close]

BLAST of Spg016536 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 283.5 bits (724), Expect = 2.1e-76
Identity = 146/318 (45.91%), Postives = 206/318 (64.78%), Query Frame = 0


Query: 4   TVGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNED 63
           ++G+NYGL GDNLP P++VI LY    I  +RIF+PN  VL+A++G  ++ + +G R++D
Sbjct: 42  SIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQD 101

Query: 64  IEGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQAL-- 123
           +   + S  A   W   ++ PY   +NIA+ITVGNEV+PG      +   +  +MQ+L  
Sbjct: 102 LAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGP-----IGPQVLPVMQSLTN 161

Query: 124 VTAGVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPY 183
           +       I ++TVVAM  L  SYPPSAG F+  A   L  ++++L+ + +PIL+ +YPY
Sbjct: 162 LVKSRNLPISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPY 221

Query: 184 FAYASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISE 243
           F+YAS+P  I LDYA F + + V  DG+L Y N+FDA+ D+F  A+EK G   + + +SE
Sbjct: 222 FSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSE 281

Query: 244 TGWPSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAE 303
           TGWPS GN   T+ D A TYN+NFVKH+ SG GTP+RPN   DG LF  FNE+ K  G E
Sbjct: 282 TGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTE 341

Query: 304 QNFGLFYP-DTHPVYPLW 319
           QNFGL+ P D  P+Y L+
Sbjct: 342 QNFGLYNPNDMKPIYNLF 354

[Close]

BLAST of Spg016536 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 2.2e-73
Identity = 141/315 (44.76%), Postives = 198/315 (62.86%), Query Frame = 0


Query: 5   VGVNYGLNGDNLPVPTEVINLYGRCGIDIVRIFEPNHGVLDAMQGKGNLVLWLGTRNEDI 64
           +G+NYGL GDNLP P+EVINLY    +  +RIF+    VL+A +G  N+ + +  +N+D+
Sbjct: 33  IGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVLNAFRGNRNIGVMVDVKNQDL 92

Query: 65  EGFATSPMAASVWVNAHVVPYYEKVNIAYITVGNEVVPGDAAAPFVANAIKNIMQALVTA 124
           E  + S  A + W   ++ PY   VNI +I VGNEV+PG+  + +V   +K++   + + 
Sbjct: 93  EALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGEIGS-YVLPVMKSLTNIVKSR 152

Query: 125 GVKTDIKVTTVVAMTALAVSYPPSAGAFSDAAAGPLKDIIQVLASSGAPILINVYPYFAY 184
            +   I ++T VAMT L  SYPPSAG F   A   L  +++ L+ +  PIL+N+YPYFAY
Sbjct: 153 SL--PILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPVLKFLSQTNTPILVNIYPYFAY 212

Query: 185 ASNPGQISLDYALFRSTSPVEVDGNLNYYNLFDAMVDSFYAALEKIGGGGVGIGISETGW 244
           A++P  I LDYA+F +   V  DG L Y N+FD + D+F  A+EK G   + + ++ETGW
Sbjct: 213 AADPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAFVWAMEKEGVKDLPMVVTETGW 272

Query: 245 PSKGNEPFTSIDNAETYNKNFVKHVTSGIGTPRRPNVKYDGILFEMFNEDLKAAGAEQNF 304
           PS GN   T+ D A  YN NFVKHV SG GTP+RP     G LF  FNE+ K AG EQNF
Sbjct: 273 PSAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGISGFLFATFNENQKPAGTEQNF 332

Query: 305 GLFYP-DTHPVYPLW 319
           GL+ P D  P+Y ++
Sbjct: 333 GLYNPTDMKPIYKMF 344

The following BLAST results are available for this feature:

BLAST of Spg016536 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896614.1	1.0e-149	80.94	glucan endo-1,3-beta-glucosidase-like [Benincasa hispida]	[more]
KAG6591759.1	1.0e-149	80.31	Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022935980.1	5.1e-149	80.00	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata]	[more]
XP_023536146.1	5.7e-148	79.62	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita pepo subsp. pepo]	[more]
XP_016899853.1	2.8e-147	79.06	PREDICTED: probable glucan endo-1,3-beta-glucosidase BG4 [Cucumis melo]	[more]

BLAST of Spg016536 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22317	4.2e-85	49.36	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
O49352	1.9e-77	47.48	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49353	3.0e-75	45.91	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q02439	9.9e-71	46.69	Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4...	[more]
Q03773	1.3e-67	45.86	Glucan endo-1,3-beta-glucosidase OS=Glycine max OX=3847 PE=1 SV=1	[more]

BLAST of Spg016536 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FC84	2.5e-149	80.00	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 P...	[more]
A0A5A7V7B6	1.4e-147	79.06	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3D518	1.4e-147	79.06	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S4DV56	1.4e-147	79.06	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487671 PE=3 SV...	[more]
A0A0A0L3A5	2.3e-147	78.75	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G377200 PE=3...	[more]

BLAST of Spg016536 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77780.1	2.2e-81	48.41	Glycosyl hydrolase superfamily protein	[more]
AT5G20330.1	1.3e-78	47.48	beta-1,3-glucanase 4	[more]
AT1G77790.1	1.6e-76	48.72	Glycosyl hydrolase superfamily protein	[more]
AT5G20340.1	2.1e-76	45.91	beta-1,3-glucanase 5	[more]
AT5G20390.1	2.2e-73	44.76	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 4..319 e-value: 6.2E-114 score: 382.2
None	No IPR available	PANTHER	PTHR32227:SF248	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 4..317
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 5..317 e-value: 7.5E-90 score: 301.7
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 4..317
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 5..317

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016536.1	Spg016536.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds