Spg016528 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016528 vs. NCBI nr
Match: XP_023536566.1 (carbon catabolite repressor protein 4 homolog 4 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.5 bits (1869), Expect = 4.7e-205
Identity = 350/392 (89.29%), Postives = 371/392 (94.64%), Query Frame = 0

Query: 1   MLAAFSSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYN 60
           MLAAF SLP LP+R++TRNIVRNMS VQ+PKHPKFISVEGADIYSRSK DGIRFRFVSYN
Sbjct: 1   MLAAFFSLPQLPVRAITRNIVRNMSGVQAPKHPKFISVEGADIYSRSKSDGIRFRFVSYN 60

Query: 61  ILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120
           ILAQVYVKSSFFPHSP  CL+WKARSQAILAVLKNLEADFLCLQE+DEYDSFYKG LERC
Sbjct: 61  ILAQVYVKSSFFPHSPSSCLRWKARSQAILAVLKNLEADFLCLQEIDEYDSFYKGTLERC 120

Query: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTR 180
           GYSSLYIQRSGQKRDGCGIFFKHENAELIIED+IEYNDLV+S+QD D SCE+KSE VVT 
Sbjct: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDRIEYNDLVNSIQDDDCSCEEKSENVVTS 180

Query: 181 ATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYW 240
           A++DVES  G SPKTT+ADRGDPNDPRVRLKRDCVGIMAAF+LKKPFHHVVIVANTHLYW
Sbjct: 181 ASHDVESNKGSSPKTTLADRGDPNDPRVRLKRDCVGIMAAFRLKKPFHHVVIVANTHLYW 240

Query: 241 DPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTS 300
           DPEWADVKLAQAKYLLSRLARFKTLV+EKFECTPS+LLAGDFNSTPGDKVYQYLVSG +S
Sbjct: 241 DPEWADVKLAQAKYLLSRLARFKTLVSEKFECTPSILLAGDFNSTPGDKVYQYLVSGKSS 300

Query: 301 SGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELP 360
           SGFSPECLEELPLPL SVYAT LG EPSFTNFTPGFTGTLDYIF SPSDSIRPIS LELP
Sbjct: 301 SGFSPECLEELPLPLSSVYATTLGREPSFTNFTPGFTGTLDYIFFSPSDSIRPISFLELP 360

Query: 361 ESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           ESE PE++GGLPN+SYPSDHLPIGA+FEITME
Sbjct: 361 ESECPEVIGGLPNFSYPSDHLPIGADFEITME 392

BLAST of Spg016528 vs. NCBI nr
Match: KAG7024715.1 (Carbon catabolite repressor protein 4-like 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 722.6 bits (1864), Expect = 1.8e-204
Identity = 349/392 (89.03%), Postives = 370/392 (94.39%), Query Frame = 0

Query: 1   MLAAFSSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYN 60
           MLAAF SLP LP+R++TRNIVRNM+ VQ+PKHPKFISVEGADIYSRSK DGIRFRFVSYN
Sbjct: 1   MLAAFFSLPQLPLRAITRNIVRNMTGVQAPKHPKFISVEGADIYSRSKSDGIRFRFVSYN 60

Query: 61  ILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120
           ILAQVYVKSSFFPHSP  CL+WKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC
Sbjct: 61  ILAQVYVKSSFFPHSPSSCLRWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120

Query: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTR 180
           GYSSLYIQRSGQKRDGCGIFFKHENAELIIED+IEYNDLV+S+QD D SCE KSE VVT 
Sbjct: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDRIEYNDLVNSIQDDDCSCEKKSENVVTS 180

Query: 181 ATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYW 240
           A++DVES  G SPKTT+ADRGDPNDPRVRLKRDCVGIMAAF+ KKPFHHVVIVANTHLYW
Sbjct: 181 ASHDVESNKGSSPKTTLADRGDPNDPRVRLKRDCVGIMAAFRFKKPFHHVVIVANTHLYW 240

Query: 241 DPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTS 300
           DPEWADVKLAQAKYLLSRLARFKTLV+EKFECTPS+LLAGDFNSTPGDKVYQYL+SG +S
Sbjct: 241 DPEWADVKLAQAKYLLSRLARFKTLVSEKFECTPSILLAGDFNSTPGDKVYQYLISGKSS 300

Query: 301 SGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELP 360
           SGFSPECLEELPLPL SVYAT LG EPSFTNFTPGFTGTLDYIF SPSDSIRPIS LELP
Sbjct: 301 SGFSPECLEELPLPLSSVYATTLGREPSFTNFTPGFTGTLDYIFFSPSDSIRPISFLELP 360

Query: 361 ESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           ESE PE++GGLPN+SYPSDHLPIGA+FEITME
Sbjct: 361 ESECPEVIGGLPNFSYPSDHLPIGADFEITME 392

BLAST of Spg016528 vs. NCBI nr
Match: KAG6591850.1 (Carbon catabolite repressor protein 4-like 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 720.7 bits (1859), Expect = 6.8e-204
Identity = 348/392 (88.78%), Postives = 370/392 (94.39%), Query Frame = 0

Query: 1   MLAAFSSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYN 60
           MLAAF SLP LP+R++TRNIVRNMS VQ+PKHPKFISVEGADIYSRSK DGIRFRFVSYN
Sbjct: 1   MLAAFFSLPQLPLRAITRNIVRNMSGVQAPKHPKFISVEGADIYSRSKSDGIRFRFVSYN 60

Query: 61  ILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120
           ILAQVYVKSSFFPHSP  CL+WKARSQAILAVLK+LEADFLCLQEVDEYDSFYKGNLERC
Sbjct: 61  ILAQVYVKSSFFPHSPSSCLRWKARSQAILAVLKSLEADFLCLQEVDEYDSFYKGNLERC 120

Query: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTR 180
           GYSSLYIQRSGQKRDGCGIFFKHENAELIIED+IEYNDLV+S+QD D SCE KSE VVT 
Sbjct: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDRIEYNDLVNSIQDDDCSCEKKSENVVTS 180

Query: 181 ATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYW 240
           A++DVES  G SPKTT+ADRGDPNDPRVRLKRDCVGIMAAF+ KKPFHHVVIVANTHLYW
Sbjct: 181 ASHDVESNKGSSPKTTLADRGDPNDPRVRLKRDCVGIMAAFRFKKPFHHVVIVANTHLYW 240

Query: 241 DPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTS 300
           DPEWADVKLAQAKYLLSRLARFKTLV+EKFECTPS+LLAGDFNSTPGDKVYQYL+SG +S
Sbjct: 241 DPEWADVKLAQAKYLLSRLARFKTLVSEKFECTPSILLAGDFNSTPGDKVYQYLISGKSS 300

Query: 301 SGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELP 360
           SGFSPECLEELPLPL SVYAT LG EPSFTNFTPGFTGTLDYIF SPSDSIRPIS LELP
Sbjct: 301 SGFSPECLEELPLPLSSVYATTLGREPSFTNFTPGFTGTLDYIFFSPSDSIRPISFLELP 360

Query: 361 ESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           ESE PE++GGLPN+SYPSDHLPIGA+F+ITME
Sbjct: 361 ESECPEVIGGLPNFSYPSDHLPIGADFDITME 392

BLAST of Spg016528 vs. NCBI nr
Match: XP_022936761.1 (carbon catabolite repressor protein 4 homolog 4 isoform X1 [Cucurbita moschata])

HSP 1 Score: 719.5 bits (1856), Expect = 1.5e-203
Identity = 347/392 (88.52%), Postives = 369/392 (94.13%), Query Frame = 0

Query: 1   MLAAFSSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYN 60
           MLAAF SLP LP+R++TRNIVRNM+ VQ+PKHPKFISVEGADIYSRSK DGIRFRFVSYN
Sbjct: 1   MLAAFFSLPQLPLRAITRNIVRNMTGVQAPKHPKFISVEGADIYSRSKSDGIRFRFVSYN 60

Query: 61  ILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120
           ILAQVYVKSSFFPHSP  CL+WKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC
Sbjct: 61  ILAQVYVKSSFFPHSPSSCLRWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120

Query: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTR 180
           GYSSLYIQRSGQKRDGCGIFFKHENAELIIED+IEYNDLV+S+QD D SCE+KSE VVT 
Sbjct: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDRIEYNDLVNSIQDDDCSCEEKSENVVTS 180

Query: 181 ATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYW 240
           A++DVES  G SPKTT+ D GDPNDPRVRLKRDCVGIMAAF+ KKPFHHVVIVANTHLYW
Sbjct: 181 ASHDVESNKGSSPKTTLPDSGDPNDPRVRLKRDCVGIMAAFRFKKPFHHVVIVANTHLYW 240

Query: 241 DPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTS 300
           DPEWADVKLAQAKYLLSRLARFKTLV+EKFECTPS+LLAGDFNSTPGDKVYQYL+SG +S
Sbjct: 241 DPEWADVKLAQAKYLLSRLARFKTLVSEKFECTPSILLAGDFNSTPGDKVYQYLISGKSS 300

Query: 301 SGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELP 360
           SGFSPECLEELPLPL SVYAT LG EPSFTNFTPGFTGTLDYIF SPSDSIRPIS LELP
Sbjct: 301 SGFSPECLEELPLPLSSVYATTLGREPSFTNFTPGFTGTLDYIFFSPSDSIRPISFLELP 360

Query: 361 ESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           ESE PE++GGLPN+SYPSDHLPIGA+FEITME
Sbjct: 361 ESECPEVIGGLPNFSYPSDHLPIGADFEITME 392

BLAST of Spg016528 vs. NCBI nr
Match: XP_008444261.1 (PREDICTED: carbon catabolite repressor protein 4 homolog 4 [Cucumis melo])

HSP 1 Score: 718.8 bits (1854), Expect = 2.6e-203
Identity = 348/387 (89.92%), Postives = 365/387 (94.32%), Query Frame = 0

Query: 6   SSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYNILAQV 65
           SSL   PIR LTRNI+RNMS VQSPKHPKFISVEGADIYSRSK DGIRFRFVSYNILAQV
Sbjct: 7   SSLHQFPIRFLTRNILRNMSAVQSPKHPKFISVEGADIYSRSKSDGIRFRFVSYNILAQV 66

Query: 66  YVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERCGYSSL 125
           YVKSSFFPHSP  CLKWKARSQAILAVLKNLEADFLCLQEVDEYD+FYKGNLERCGYSSL
Sbjct: 67  YVKSSFFPHSPSSCLKWKARSQAILAVLKNLEADFLCLQEVDEYDTFYKGNLERCGYSSL 126

Query: 126 YIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRATNDV 185
           YIQRSGQKRDGCGIFFKHE AELIIED+IEYNDLVDS+QD   SCEDKSE+VVT A+NDV
Sbjct: 127 YIQRSGQKRDGCGIFFKHEKAELIIEDRIEYNDLVDSIQDDSGSCEDKSEDVVTSASNDV 186

Query: 186 ESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYWDPEWA 245
           ES  G SPKTTVA RGDPNDPRVRLKRDCVGIMAAFKL++PFHHVVIVANTHLYWDPEWA
Sbjct: 187 ESNKGSSPKTTVAYRGDPNDPRVRLKRDCVGIMAAFKLEQPFHHVVIVANTHLYWDPEWA 246

Query: 246 DVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTSSGFSP 305
           DVKLAQAKYLLSRLARFK+LVAEKFECTPS+LLAGDFNSTPGDKVYQYL+SGN+SSGFSP
Sbjct: 247 DVKLAQAKYLLSRLARFKSLVAEKFECTPSILLAGDFNSTPGDKVYQYLISGNSSSGFSP 306

Query: 306 ECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWP 365
           ECLEELPLPL SVYA ILGSEPSFTNFTPGFTGTLDYIF SPSDSIRP+S LELPES+WP
Sbjct: 307 ECLEELPLPLCSVYANILGSEPSFTNFTPGFTGTLDYIFFSPSDSIRPVSFLELPESDWP 366

Query: 366 EILGGLPNYSYPSDHLPIGAEFEITME 393
           EI+GGLPN+SYPSDHLPI A+FEITME
Sbjct: 367 EIIGGLPNHSYPSDHLPIAADFEITME 393

BLAST of Spg016528 vs. ExPASy Swiss-Prot
Match: A8MS41 (Carbon catabolite repressor protein 4 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=CCR4-4 PE=1 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 4.4e-129
Identity = 245/418 (58.61%), Postives = 293/418 (70.10%), Query Frame = 0

Query: 10  LLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPD------------------- 69
           LLP + ++R +  N +    PK  KF SVEG DI SR+K D                   
Sbjct: 16  LLPRKVISRRMSTNPAI--EPKVRKFESVEGVDIGSRNKSDGFFAIPLYLSKLVALYNCI 75

Query: 70  ---------------GIRFRFVSYNILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKN 129
                          GIRFR VSYNILAQVYVKS+  PHSPP CLKWKARS AIL+VLKN
Sbjct: 76  SLSRIGTSNENFVFSGIRFRLVSYNILAQVYVKSALLPHSPPACLKWKARSHAILSVLKN 135

Query: 130 LEADFLCLQEVDEYDSFYKGNLERCGYSSLYIQRSGQ-KRDGCGIFFKHENAELIIEDKI 189
           L+ADF CLQEVDEYDSFY+ N++  GYS +YIQR+GQ KRDGC IF+K   AEL+ +++I
Sbjct: 136 LQADFFCLQEVDEYDSFYRNNMDSLGYSGIYIQRTGQRKRDGCAIFYKPSCAELVTKERI 195

Query: 190 EYNDLVDSLQDGDRSCEDKSEEVVTRATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDC 249
           EYNDLVDS++    SC ++           +E+ N    K +  D  D NDP VRLKRDC
Sbjct: 196 EYNDLVDSIKADSVSCSEQK----------IETSN--EGKDSRKDSRDLNDPLVRLKRDC 255

Query: 250 VGIMAAFKLKKPFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFKTLVAEKFECTP 309
           VGIMAAF++ KPF H+VIVANTHLYWDPE ADVKLAQAKYLLSRLA+FKTL++++FECTP
Sbjct: 256 VGIMAAFRINKPFQHIVIVANTHLYWDPELADVKLAQAKYLLSRLAQFKTLISDEFECTP 315

Query: 310 SVLLAGDFNSTPGDKVYQYLVSGNTSSGFSPECLEELPLPLRSVYATILGSEPSFTNFTP 369
           S+LLAGDFNS PGD VY YLVSGN     + E  EE P+PL SVY    G EP FTN TP
Sbjct: 316 SLLLAGDFNSIPGDMVYSYLVSGNAKPTETIE-EEEAPVPLSSVYEVTRG-EPKFTNCTP 375

Query: 370 GFTGTLDYIFLSPSDSIRPISLLELPESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           GFT TLDYIF+SPSD I+P+S+L+LPE + P+++G LPN+ +PSDHLPIGAEFEI  E
Sbjct: 376 GFTNTLDYIFISPSDFIKPVSILQLPEPDSPDVVGFLPNHHHPSDHLPIGAEFEIRRE 417

BLAST of Spg016528 vs. ExPASy Swiss-Prot
Match: O74874 (CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ccr4 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.3e-32
Identity = 106/383 (27.68%), Postives = 168/383 (43.86%), Query Frame = 0

Query: 52  IRFRFVSYNILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVD--EY 111
           ++F  +SYN+L + Y  S+ + ++P   L W  R   I+  L    AD +CLQEVD   Y
Sbjct: 334 LKFTIMSYNVLCERYATSTLYGYTPSWALSWSYRKDLIMQELGGYNADIICLQEVDVENY 393

Query: 112 DSFYKGNLERCGYSSLYIQRSGQKR---------DGCGIFFKHENAELIIEDKIEYNDLV 171
           D+F+   +   GY  ++  +S  +          DGC  FFK     +  +  IEYN   
Sbjct: 394 DTFFAPQMSLKGYKGVHFPKSRVRTMNEVERRIVDGCATFFKTSKYVMHEKMVIEYNQA- 453

Query: 172 DSLQDGDRSCEDKSEEVVTRATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAA 231
                   S   +  ++ +   N V +K+ +S  T + ++   N  R             
Sbjct: 454 -------PSLRRQDIKLTSNMYNRVMTKDNISVITLLENK--ENGSR------------- 513

Query: 232 FKLKKPFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPS----- 291
                     +IVAN H++WDP++ DVK+ Q   L+  +A+    VA KF   PS     
Sbjct: 514 ----------LIVANCHIHWDPQFRDVKVIQVAMLMDEIAQ----VATKFRNMPSKIPSD 573

Query: 292 ----------------VLLAGDFNSTPGDKVYQYLVSGNTSSGFSPECLEEL-------- 351
                           +L+ GDFNS  G  VY +L SG+ S         +         
Sbjct: 574 QLKDERPTYPEYLKIPILICGDFNSVQGSGVYDFLSSGSISQNHEDFMNNDYGEYTVNGR 633

Query: 352 --PLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWPEILG 393
                L+S Y        SFTN+TPGF G +D+I+ +  +S+    LL+  + ++   + 
Sbjct: 634 SHAFNLKSAYGE--SEALSFTNYTPGFKGAIDHIWYT-GNSLEVTGLLKGVDKDYLSGVV 676

BLAST of Spg016528 vs. ExPASy Swiss-Prot
Match: C4V7I7 (Probable CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 OS=Nosema ceranae (strain BRL01) OX=578460 GN=CCR4 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.9e-32
Identity = 110/356 (30.90%), Postives = 166/356 (46.63%), Query Frame = 0

Query: 52  IRFRFVSYNILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVD--EY 111
           I F   +YNIL+        +P  P   L    R + IL  + ++  D LCLQEV+   Y
Sbjct: 169 ITFSCGTYNILSNYSAVRLGYP--PTWVLNPDYRKENILHNICSINVDILCLQEVETYNY 228

Query: 112 DSFYKGNLE-RCGYSSLYIQRSGQKR-------DGCGIFFKHENAELIIEDKIEYNDLVD 171
           + FYK  LE RC YSS++  +   K        DGC  F+K        + KI+ N ++D
Sbjct: 229 EDFYKDQLELRCEYSSVFQPKGRSKNLTDSKSVDGCATFWKKS------KFKIKENLVID 288

Query: 172 SLQDGDRSCEDKSEEVVTRATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAF 231
                            ++  ND      ++    V+  G         K+D + +++ F
Sbjct: 289 ---------------FYSKFINDYRFNKNIN---LVSRYG---------KKDNIALISIF 348

Query: 232 KLKKPFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGD 291
           ++ +     +IV N HLYWDPE+ D+K  QA  LL  L +       K    PS++L GD
Sbjct: 349 EISQT-KQTLIVVNVHLYWDPEYEDIKFVQAIILLEELEK-----VSKCYKNPSIVLLGD 408

Query: 292 FNSTPGDKVYQYLVSGNTSS--------GFSPECLEELPLPLRSVYATILGSEPSFTNFT 351
           FNS     VY ++   + S+        GF P       L L   Y   L  E  FTNFT
Sbjct: 409 FNSLQNSSVYSFITQNSVSNTNLCKYNIGFIPGHF----LKLSDAY---LSEENDFTNFT 468

Query: 352 PGFTGTLDYIFLSPSDSIRPISLLELPESEWPEILGGLPNYSYPSDHLPIGAEFEI 390
           P F G +D+IF   SD++   S+L   E+E+ + + GLPN  +PSDH+ + ++F++
Sbjct: 469 PTFKGVIDFIFY--SDTLELRSILSTIENEYCDQVVGLPNIHFPSDHIFLASKFKL 474

BLAST of Spg016528 vs. ExPASy Swiss-Prot
Match: Q1EA11 (CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 OS=Coccidioides immitis (strain RS) OX=246410 GN=CCR4 PE=3 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 4.2e-31
Identity = 114/438 (26.03%), Postives = 184/438 (42.01%), Query Frame = 0

Query: 14  RSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYNILAQVYVKSSFFP 73
           +SL + +   M     P +  ++ ++     S +  +  +F  ++YN L   Y  +  + 
Sbjct: 343 KSLIKYLKEEMPVHLPPSNRDWLILDETGKNSANGGNDNKFTALTYNTLCDRYATNQQYG 402

Query: 74  HSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDE--YDSFYKGNLERCGYSSLY----- 133
           ++P   L W+ R   +L  ++  +AD +CLQE+D+  Y  F++  L    Y  +Y     
Sbjct: 403 YAPSRALAWEFRRDLLLNEIRGHDADIVCLQEIDQGSYHGFFREQLAYNDYKGVYWPKGR 462

Query: 134 ----IQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRAT 193
                +   +  DGC  FFK     L+ ++ I +       Q   R  + K ++      
Sbjct: 463 AQGMPEEEAKLVDGCATFFKGSKYILLEKNMIHFG------QTAVRRPDAKGQD------ 522

Query: 194 NDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYWDP 253
              +  N L  K  +A                   +  F   +     +IV N H+YWDP
Sbjct: 523 ---DIYNRLWQKDNIA-------------------VIVFLENRLTGERLIVVNAHIYWDP 582

Query: 254 EWADVKLAQAKYLLSRLARFKTLVAEKF------------------------------EC 313
            + DVKL Q   ++  +    T +AEK+                              E 
Sbjct: 583 AYKDVKLIQVAIMMEEV----TQLAEKYVKIPPCTDKTAFRFSEPEDGKESQGTSTPVEP 642

Query: 314 TPSV----------LLAGDFNSTPGDKVYQYLVSGNTSSGFSPECLEEL----------- 373
            PSV          L+ GDFNS PG  VY  L  G  +    P+  + L           
Sbjct: 643 APSVEYSSASQIPILVCGDFNSCPGSAVYNLLAHGRMAEE-HPDLEQRLYGNLSRMGMSH 702

Query: 374 PLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWPEILGGL 390
           P  L+S Y+TI   E SFTN+TPGFT  +DYI+ S S++++  +LL   + E+ + + G 
Sbjct: 703 PFTLKSAYSTI--GELSFTNYTPGFTDVIDYIWYS-SNTLQVTALLGEVDKEYLKRVPGF 738

BLAST of Spg016528 vs. ExPASy Swiss-Prot
Match: Q8SU52 (Probable CCR4-Not complex 3'-5'-exoribonuclease subunit Ccr4 OS=Encephalitozoon cuniculi (strain GB-M1) OX=284813 GN=CCR4 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.1e-30
Identity = 103/348 (29.60%), Postives = 160/348 (45.98%), Query Frame = 0

Query: 58  SYNILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYD--SFYKG 117
           ++NIL+ +Y  ++   ++P   +  + R + +L  +     D LCLQE++ Y    FYK 
Sbjct: 176 TFNILSNIY--ATRMTYAPSWVINSEFRREGVLQEIVLYNVDILCLQEIELYSFFDFYKE 235

Query: 118 NLE-RCGYSSLYIQRSGQKR-------DGCGIFFKHENAELIIEDKIEYNDLVDSLQDGD 177
            LE RC Y S+   R   K        DGC IF++     LI +  I+++  V  +QD  
Sbjct: 236 QLEMRCNYDSIIYPRGRVKSVPDKKNVDGCAIFWRRSKFRLIAQFPIDFHQKV--IQD-- 295

Query: 178 RSCEDKSEEVVTRATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPF 237
                      TR   + E  +    K  +A                +G +    L++P 
Sbjct: 296 -----------TRFNTNQELLDRYGKKDNIA----------------IGAL----LERPN 355

Query: 238 HHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPG 297
              V+V NTH++WDP++ D+KL Q   L+  + R  +           +LL GDFNS   
Sbjct: 356 GQQVLVMNTHIFWDPDYPDIKLLQVLLLVEEIKRVSSR-----HPNACLLLQGDFNSLRS 415

Query: 298 DKVYQYLVS--------GNTSSGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGT 357
             VY+ + +        G+T    S +   +  L L   Y+     +  FTNFTPGF G 
Sbjct: 416 SSVYKSITTPVIDFADFGDTMQHLSNQQFGD-GLGLNDAYS---NQDLGFTNFTPGFKGV 475

Query: 358 LDYIFLSPSDSIRPISLLELPESEWPEILGGLPNYSYPSDHLPIGAEF 388
           +DYIF     S+   S+L   E E+ E + GLPN  +PSDH+ +GA+F
Sbjct: 476 IDYIFYGGGISL--ASVLSPVEDEYTENVAGLPNMHFPSDHIFLGAKF 475

BLAST of Spg016528 vs. ExPASy TrEMBL
Match: A0A6J1F8E0 (carbon catabolite repressor protein 4 homolog 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443256 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 7.4e-204
Identity = 347/392 (88.52%), Postives = 369/392 (94.13%), Query Frame = 0

Query: 1   MLAAFSSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYN 60
           MLAAF SLP LP+R++TRNIVRNM+ VQ+PKHPKFISVEGADIYSRSK DGIRFRFVSYN
Sbjct: 1   MLAAFFSLPQLPLRAITRNIVRNMTGVQAPKHPKFISVEGADIYSRSKSDGIRFRFVSYN 60

Query: 61  ILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120
           ILAQVYVKSSFFPHSP  CL+WKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC
Sbjct: 61  ILAQVYVKSSFFPHSPSSCLRWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120

Query: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTR 180
           GYSSLYIQRSGQKRDGCGIFFKHENAELIIED+IEYNDLV+S+QD D SCE+KSE VVT 
Sbjct: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDRIEYNDLVNSIQDDDCSCEEKSENVVTS 180

Query: 181 ATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYW 240
           A++DVES  G SPKTT+ D GDPNDPRVRLKRDCVGIMAAF+ KKPFHHVVIVANTHLYW
Sbjct: 181 ASHDVESNKGSSPKTTLPDSGDPNDPRVRLKRDCVGIMAAFRFKKPFHHVVIVANTHLYW 240

Query: 241 DPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTS 300
           DPEWADVKLAQAKYLLSRLARFKTLV+EKFECTPS+LLAGDFNSTPGDKVYQYL+SG +S
Sbjct: 241 DPEWADVKLAQAKYLLSRLARFKTLVSEKFECTPSILLAGDFNSTPGDKVYQYLISGKSS 300

Query: 301 SGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELP 360
           SGFSPECLEELPLPL SVYAT LG EPSFTNFTPGFTGTLDYIF SPSDSIRPIS LELP
Sbjct: 301 SGFSPECLEELPLPLSSVYATTLGREPSFTNFTPGFTGTLDYIFFSPSDSIRPISFLELP 360

Query: 361 ESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           ESE PE++GGLPN+SYPSDHLPIGA+FEITME
Sbjct: 361 ESECPEVIGGLPNFSYPSDHLPIGADFEITME 392

BLAST of Spg016528 vs. ExPASy TrEMBL
Match: A0A1S3B9X8 (carbon catabolite repressor protein 4 homolog 4 OS=Cucumis melo OX=3656 GN=LOC103487637 PE=4 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.3e-203
Identity = 348/387 (89.92%), Postives = 365/387 (94.32%), Query Frame = 0

Query: 6   SSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYNILAQV 65
           SSL   PIR LTRNI+RNMS VQSPKHPKFISVEGADIYSRSK DGIRFRFVSYNILAQV
Sbjct: 7   SSLHQFPIRFLTRNILRNMSAVQSPKHPKFISVEGADIYSRSKSDGIRFRFVSYNILAQV 66

Query: 66  YVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERCGYSSL 125
           YVKSSFFPHSP  CLKWKARSQAILAVLKNLEADFLCLQEVDEYD+FYKGNLERCGYSSL
Sbjct: 67  YVKSSFFPHSPSSCLKWKARSQAILAVLKNLEADFLCLQEVDEYDTFYKGNLERCGYSSL 126

Query: 126 YIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRATNDV 185
           YIQRSGQKRDGCGIFFKHE AELIIED+IEYNDLVDS+QD   SCEDKSE+VVT A+NDV
Sbjct: 127 YIQRSGQKRDGCGIFFKHEKAELIIEDRIEYNDLVDSIQDDSGSCEDKSEDVVTSASNDV 186

Query: 186 ESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYWDPEWA 245
           ES  G SPKTTVA RGDPNDPRVRLKRDCVGIMAAFKL++PFHHVVIVANTHLYWDPEWA
Sbjct: 187 ESNKGSSPKTTVAYRGDPNDPRVRLKRDCVGIMAAFKLEQPFHHVVIVANTHLYWDPEWA 246

Query: 246 DVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTSSGFSP 305
           DVKLAQAKYLLSRLARFK+LVAEKFECTPS+LLAGDFNSTPGDKVYQYL+SGN+SSGFSP
Sbjct: 247 DVKLAQAKYLLSRLARFKSLVAEKFECTPSILLAGDFNSTPGDKVYQYLISGNSSSGFSP 306

Query: 306 ECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWP 365
           ECLEELPLPL SVYA ILGSEPSFTNFTPGFTGTLDYIF SPSDSIRP+S LELPES+WP
Sbjct: 307 ECLEELPLPLCSVYANILGSEPSFTNFTPGFTGTLDYIFFSPSDSIRPVSFLELPESDWP 366

Query: 366 EILGGLPNYSYPSDHLPIGAEFEITME 393
           EI+GGLPN+SYPSDHLPI A+FEITME
Sbjct: 367 EIIGGLPNHSYPSDHLPIAADFEITME 393

BLAST of Spg016528 vs. ExPASy TrEMBL
Match: A0A6J1CI40 (carbon catabolite repressor protein 4 homolog 4 OS=Momordica charantia OX=3673 GN=LOC111011381 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 1.9e-199
Identity = 345/392 (88.01%), Postives = 362/392 (92.35%), Query Frame = 0

Query: 1   MLAAFSSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYN 60
           MLA + +LP LPIRSLTR IV NMSTVQSPK+PKFISVEGADIYSRSKPDGIRFR VSYN
Sbjct: 1   MLAPYLTLPQLPIRSLTRQIVHNMSTVQSPKYPKFISVEGADIYSRSKPDGIRFRLVSYN 60

Query: 61  ILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120
           ILAQVYVKSSFFPHSP PCLKWKARS AILAVLKNLEADFLCLQEVDEYDSFYKGNLERC
Sbjct: 61  ILAQVYVKSSFFPHSPSPCLKWKARSLAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120

Query: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTR 180
           GYSSLYIQRSGQKRDGCGIFFKHENAELIIED+IEYNDLVDS+QD   SCEDKSE+VVT 
Sbjct: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDRIEYNDLVDSIQDDGCSCEDKSEDVVTG 180

Query: 181 ATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYW 240
           A+NDVES NGLS KTT  D GDPNDPRVRLKRDCVGIMAAFKLKKPFHH+VIVANTHLYW
Sbjct: 181 ASNDVESNNGLSTKTTAGDHGDPNDPRVRLKRDCVGIMAAFKLKKPFHHIVIVANTHLYW 240

Query: 241 DPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTS 300
           DPEWADVKLAQAKYLLSRLARF+TLV+EKFEC PSVLLAGDFNSTPGDKVYQYLVSG+  
Sbjct: 241 DPEWADVKLAQAKYLLSRLARFRTLVSEKFECKPSVLLAGDFNSTPGDKVYQYLVSGD-- 300

Query: 301 SGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELP 360
              SPECLEELPLPL SVY+TILG+EPSFTN TPGFTGTLDYIF SPSD IRPIS LELP
Sbjct: 301 ---SPECLEELPLPLCSVYSTILGTEPSFTNCTPGFTGTLDYIFFSPSDPIRPISFLELP 360

Query: 361 ESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           +SEWPE++GGLPN +YPSDHLPI AEFEITME
Sbjct: 361 QSEWPEVIGGLPNNTYPSDHLPIAAEFEITME 387

BLAST of Spg016528 vs. ExPASy TrEMBL
Match: A0A5A7UEY1 (Carbon catabolite repressor protein 4-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold207G001030 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 3.9e-197
Identity = 335/369 (90.79%), Postives = 351/369 (95.12%), Query Frame = 0

Query: 24  MSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYNILAQVYVKSSFFPHSPPPCLKWK 83
           MS VQSPKHPKFISVEGADIYSRSK DGIRFRFVSYNILAQVYVKSSFFPHSP  CLKWK
Sbjct: 1   MSAVQSPKHPKFISVEGADIYSRSKSDGIRFRFVSYNILAQVYVKSSFFPHSPSSCLKWK 60

Query: 84  ARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERCGYSSLYIQRSGQKRDGCGIFFKH 143
           ARSQAILAVLKNLEADFLCLQEVDEYD+FYKGNLERCGYSSLYIQRSGQKRDGCGIFFKH
Sbjct: 61  ARSQAILAVLKNLEADFLCLQEVDEYDTFYKGNLERCGYSSLYIQRSGQKRDGCGIFFKH 120

Query: 144 ENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRATNDVESKNGLSPKTTVADRGDP 203
           E AELIIED+IEYNDLVDS+QD   SCEDKSE+VVT A+NDVES  G SPKTTVA RGDP
Sbjct: 121 EKAELIIEDRIEYNDLVDSIQDDSGSCEDKSEDVVTSASNDVESNKGSSPKTTVAYRGDP 180

Query: 204 NDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFK 263
           NDPRVRLKRDCVGIMAAFKL++PFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFK
Sbjct: 181 NDPRVRLKRDCVGIMAAFKLEQPFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFK 240

Query: 264 TLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTSSGFSPECLEELPLPLRSVYATIL 323
           +LVAEKFECTPS+LLAGDFNSTPGDKVYQYL+SGN+SSGFSPECLEELPLPL SVYA IL
Sbjct: 241 SLVAEKFECTPSILLAGDFNSTPGDKVYQYLISGNSSSGFSPECLEELPLPLCSVYANIL 300

Query: 324 GSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWPEILGGLPNYSYPSDHLPI 383
           GSEPSFTNFTPGFTGTLDYIF SPSDSIRP+S LELPES+WPEI+GGLPN+SYPSDHLPI
Sbjct: 301 GSEPSFTNFTPGFTGTLDYIFFSPSDSIRPVSFLELPESDWPEIIGGLPNHSYPSDHLPI 360

Query: 384 GAEFEITME 393
            A+FEITME
Sbjct: 361 AADFEITME 369

BLAST of Spg016528 vs. ExPASy TrEMBL
Match: A0A6J1IN76 (carbon catabolite repressor protein 4 homolog 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477068 PE=4 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.7e-197
Identity = 342/392 (87.24%), Postives = 362/392 (92.35%), Query Frame = 0

Query: 1   MLAAFSSLPLLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYN 60
           MLAAF SLP LP+R++TRNIVRNMS VQ+ KHPKFISVEGADIYSRSK DGIRFRFVSYN
Sbjct: 1   MLAAFFSLPQLPLRAITRNIVRNMSGVQALKHPKFISVEGADIYSRSKSDGIRFRFVSYN 60

Query: 61  ILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERC 120
           ILAQVYVKSSFFPHSP  CL+WKARSQAILAVLKNLEADFLCLQEVDEYDSFY GNLERC
Sbjct: 61  ILAQVYVKSSFFPHSPSSCLRWKARSQAILAVLKNLEADFLCLQEVDEYDSFYNGNLERC 120

Query: 121 GYSSLYIQRSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTR 180
           GYSSLYIQRSGQKRDGCGIFFK ENAELIIED+IEYNDLV+S+QD D SCE+ S      
Sbjct: 121 GYSSLYIQRSGQKRDGCGIFFKRENAELIIEDRIEYNDLVNSMQDDDCSCEENS------ 180

Query: 181 ATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYW 240
           A++DVES  G SPKTT+ADRGDPNDPRVRLKRDCVGIMAAF+LKKPFHHVVIVANTHLYW
Sbjct: 181 ASHDVESSKGSSPKTTLADRGDPNDPRVRLKRDCVGIMAAFRLKKPFHHVVIVANTHLYW 240

Query: 241 DPEWADVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTS 300
           DPEWADVKLAQAKYLLSRLARFKTLV+EKFECTPS+LLAGDFNSTPGDKVYQ LVSG +S
Sbjct: 241 DPEWADVKLAQAKYLLSRLARFKTLVSEKFECTPSILLAGDFNSTPGDKVYQCLVSGKSS 300

Query: 301 SGFSPECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELP 360
           S  SPECLEELPLPL SVYAT LG EPSFTNFTPGFTGTLDYIF SPSDSIRPISLLELP
Sbjct: 301 SELSPECLEELPLPLSSVYATTLGREPSFTNFTPGFTGTLDYIFFSPSDSIRPISLLELP 360

Query: 361 ESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           ESE PE++GGLPN+SYPSDHLPIGA+FEITME
Sbjct: 361 ESECPEVIGGLPNFSYPSDHLPIGADFEITME 386

BLAST of Spg016528 vs. TAIR 10
Match: AT1G31500.1 (DNAse I-like superfamily protein )

HSP 1 Score: 481.5 bits (1238), Expect = 6.5e-136
Identity = 248/387 (64.08%), Postives = 294/387 (75.97%), Query Frame = 0

Query: 10  LLPIRSLTRNIV-RNMST--VQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYNILAQVY 69
           LLP   + R ++ R MST     PK  KF SVEG DI SR+K DGIRFR VSYNILAQVY
Sbjct: 16  LLPSSRVCRKVISRRMSTNPAIEPKVRKFESVEGVDIGSRNKSDGIRFRLVSYNILAQVY 75

Query: 70  VKSSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERCGYSSLY 129
           VKS+  PHSPP CLKWKARS AIL+VLKNL+ADF CLQEVDEYDSFY+ N++  GYS +Y
Sbjct: 76  VKSALLPHSPPACLKWKARSHAILSVLKNLQADFFCLQEVDEYDSFYRNNMDSLGYSGIY 135

Query: 130 IQRSGQ-KRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRATNDV 189
           IQR+GQ KRDGC IF+K   AEL+ +++IEYNDLVDS++    SC ++           +
Sbjct: 136 IQRTGQRKRDGCAIFYKPSCAELVTKERIEYNDLVDSIKADSVSCSEQK----------I 195

Query: 190 ESKNGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYWDPEWA 249
           E+ N    K +  D  D NDP VRLKRDCVGIMAAF++ KPF H+VIVANTHLYWDPE A
Sbjct: 196 ETSN--EGKDSRKDSRDLNDPLVRLKRDCVGIMAAFRINKPFQHIVIVANTHLYWDPELA 255

Query: 250 DVKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTSSGFSP 309
           DVKLAQAKYLLSRLA+FKTL++++FECTPS+LLAGDFNS PGD VY YLVSGN     + 
Sbjct: 256 DVKLAQAKYLLSRLAQFKTLISDEFECTPSLLLAGDFNSIPGDMVYSYLVSGNAKPTETI 315

Query: 310 ECLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWP 369
           E  EE P+PL SVY    G EP FTN TPGFT TLDYIF+SPSD I+P+S+L+LPE + P
Sbjct: 316 E-EEEAPVPLSSVYEVTRG-EPKFTNCTPGFTNTLDYIFISPSDFIKPVSILQLPEPDSP 375

Query: 370 EILGGLPNYSYPSDHLPIGAEFEITME 393
           +++G LPN+ +PSDHLPIGAEFEI  E
Sbjct: 376 DVVGFLPNHHHPSDHLPIGAEFEIRRE 388

BLAST of Spg016528 vs. TAIR 10
Match: AT1G31500.2 (DNAse I-like superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.2e-134
Identity = 240/364 (65.93%), Postives = 283/364 (77.75%), Query Frame = 0

Query: 30  PKHPKFISVEGADIYSRSKPDGIRFRFVSYNILAQVYVKSSFFPHSPPPCLKWKARSQAI 89
           PK  KF SVEG DI SR+K DGIRFR VSYNILAQVYVKS+  PHSPP CLKWKARS AI
Sbjct: 9   PKVRKFESVEGVDIGSRNKSDGIRFRLVSYNILAQVYVKSALLPHSPPACLKWKARSHAI 68

Query: 90  LAVLKNLEADFLCLQEVDEYDSFYKGNLERCGYSSLYIQRSGQ-KRDGCGIFFKHENAEL 149
           L+VLKNL+ADF CLQEVDEYDSFY+ N++  GYS +YIQR+GQ KRDGC IF+K   AEL
Sbjct: 69  LSVLKNLQADFFCLQEVDEYDSFYRNNMDSLGYSGIYIQRTGQRKRDGCAIFYKPSCAEL 128

Query: 150 IIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRATNDVESKNGLSPKTTVADRGDPNDPRV 209
           + +++IEYNDLVDS++    SC ++           +E+ N    K +  D  D NDP V
Sbjct: 129 VTKERIEYNDLVDSIKADSVSCSEQK----------IETSN--EGKDSRKDSRDLNDPLV 188

Query: 210 RLKRDCVGIMAAFKLKKPFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFKTLVAE 269
           RLKRDCVGIMAAF++ KPF H+VIVANTHLYWDPE ADVKLAQAKYLLSRLA+FKTL+++
Sbjct: 189 RLKRDCVGIMAAFRINKPFQHIVIVANTHLYWDPELADVKLAQAKYLLSRLAQFKTLISD 248

Query: 270 KFECTPSVLLAGDFNSTPGDKVYQYLVSGNTSSGFSPECLEELPLPLRSVYATILGSEPS 329
           +FECTPS+LLAGDFNS PGD VY YLVSGN     + E  EE P+PL SVY    G EP 
Sbjct: 249 EFECTPSLLLAGDFNSIPGDMVYSYLVSGNAKPTETIE-EEEAPVPLSSVYEVTRG-EPK 308

Query: 330 FTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWPEILGGLPNYSYPSDHLPIGAEFE 389
           FTN TPGFT TLDYIF+SPSD I+P+S+L+LPE + P+++G LPN+ +PSDHLPIGAEFE
Sbjct: 309 FTNCTPGFTNTLDYIFISPSDFIKPVSILQLPEPDSPDVVGFLPNHHHPSDHLPIGAEFE 358

Query: 390 ITME 393
           I  E
Sbjct: 369 IRRE 358

BLAST of Spg016528 vs. TAIR 10
Match: AT1G31500.4 (DNAse I-like superfamily protein )

HSP 1 Score: 462.6 bits (1189), Expect = 3.1e-130
Identity = 245/418 (58.61%), Postives = 293/418 (70.10%), Query Frame = 0

Query: 10  LLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPD------------------- 69
           LLP + ++R +  N +    PK  KF SVEG DI SR+K D                   
Sbjct: 16  LLPRKVISRRMSTNPAI--EPKVRKFESVEGVDIGSRNKSDGFFAIPLYLSKLVALYNCI 75

Query: 70  ---------------GIRFRFVSYNILAQVYVKSSFFPHSPPPCLKWKARSQAILAVLKN 129
                          GIRFR VSYNILAQVYVKS+  PHSPP CLKWKARS AIL+VLKN
Sbjct: 76  SLSRIGTSNENFVFSGIRFRLVSYNILAQVYVKSALLPHSPPACLKWKARSHAILSVLKN 135

Query: 130 LEADFLCLQEVDEYDSFYKGNLERCGYSSLYIQRSGQ-KRDGCGIFFKHENAELIIEDKI 189
           L+ADF CLQEVDEYDSFY+ N++  GYS +YIQR+GQ KRDGC IF+K   AEL+ +++I
Sbjct: 136 LQADFFCLQEVDEYDSFYRNNMDSLGYSGIYIQRTGQRKRDGCAIFYKPSCAELVTKERI 195

Query: 190 EYNDLVDSLQDGDRSCEDKSEEVVTRATNDVESKNGLSPKTTVADRGDPNDPRVRLKRDC 249
           EYNDLVDS++    SC ++           +E+ N    K +  D  D NDP VRLKRDC
Sbjct: 196 EYNDLVDSIKADSVSCSEQK----------IETSN--EGKDSRKDSRDLNDPLVRLKRDC 255

Query: 250 VGIMAAFKLKKPFHHVVIVANTHLYWDPEWADVKLAQAKYLLSRLARFKTLVAEKFECTP 309
           VGIMAAF++ KPF H+VIVANTHLYWDPE ADVKLAQAKYLLSRLA+FKTL++++FECTP
Sbjct: 256 VGIMAAFRINKPFQHIVIVANTHLYWDPELADVKLAQAKYLLSRLAQFKTLISDEFECTP 315

Query: 310 SVLLAGDFNSTPGDKVYQYLVSGNTSSGFSPECLEELPLPLRSVYATILGSEPSFTNFTP 369
           S+LLAGDFNS PGD VY YLVSGN     + E  EE P+PL SVY    G EP FTN TP
Sbjct: 316 SLLLAGDFNSIPGDMVYSYLVSGNAKPTETIE-EEEAPVPLSSVYEVTRG-EPKFTNCTP 375

Query: 370 GFTGTLDYIFLSPSDSIRPISLLELPESEWPEILGGLPNYSYPSDHLPIGAEFEITME 393
           GFT TLDYIF+SPSD I+P+S+L+LPE + P+++G LPN+ +PSDHLPIGAEFEI  E
Sbjct: 376 GFTNTLDYIFISPSDFIKPVSILQLPEPDSPDVVGFLPNHHHPSDHLPIGAEFEIRRE 417

BLAST of Spg016528 vs. TAIR 10
Match: AT1G31500.3 (DNAse I-like superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 2.4e-98
Identity = 182/282 (64.54%), Postives = 217/282 (76.95%), Query Frame = 0

Query: 10  LLPIRSLTRNIVRNMSTVQSPKHPKFISVEGADIYSRSKPDGIRFRFVSYNILAQVYVKS 69
           LLP + ++R +  N +    PK  KF SVEG DI SR+K DGIRFR VSYNILAQVYVKS
Sbjct: 16  LLPRKVISRRMSTNPAI--EPKVRKFESVEGVDIGSRNKSDGIRFRLVSYNILAQVYVKS 75

Query: 70  SFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERCGYSSLYIQR 129
           +  PHSPP CLKWKARS AIL+VLKNL+ADF CLQEVDEYDSFY+ N++  GYS +YIQR
Sbjct: 76  ALLPHSPPACLKWKARSHAILSVLKNLQADFFCLQEVDEYDSFYRNNMDSLGYSGIYIQR 135

Query: 130 SGQ-KRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRATNDVESK 189
           +GQ KRDGC IF+K   AEL+ +++IEYNDLVDS++    SC ++           +E+ 
Sbjct: 136 TGQRKRDGCAIFYKPSCAELVTKERIEYNDLVDSIKADSVSCSEQK----------IETS 195

Query: 190 NGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLYWDPEWADVK 249
           N    K +  D  D NDP VRLKRDCVGIMAAF++ KPF H+VIVANTHLYWDPE ADVK
Sbjct: 196 N--EGKDSRKDSRDLNDPLVRLKRDCVGIMAAFRINKPFQHIVIVANTHLYWDPELADVK 255

Query: 250 LAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKV 291
           LAQAKYLLSRLA+FKTL++++FECTPS+LLAGDFNS PGD V
Sbjct: 256 LAQAKYLLSRLAQFKTLISDEFECTPSLLLAGDFNSIPGDMV 283

BLAST of Spg016528 vs. TAIR 10
Match: AT1G31530.1 (DNAse I-like superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 9.0e-53
Identity = 131/323 (40.56%), Postives = 175/323 (54.18%), Query Frame = 0

Query: 69  SSFFPHSPPPCLKWKARSQAILAVLKNLEADFLCLQEVDEYDSFYKGNLERCGYSSLYIQ 128
           SS    SPP  + W+ RS+AIL  +KN EADF+CLQEVDEY SF+  N+E  GYS + I+
Sbjct: 4   SSLMYVSPPESILWEKRSKAILDNIKNFEADFICLQEVDEYHSFFDRNMEAQGYSGIPIE 63

Query: 129 RSGQKRDGCGIFFKHENAELIIEDKIEYNDLVDSLQDGDRSCEDKSEEVVTRATNDVESK 188
              ++   C IFFK + AE I          +      +  C   S   V+  ++DV + 
Sbjct: 64  --NKEGYECAIFFKPKFAEFITYQTTR----IQGYTKYENLCVAPSSSTVSSESSDVVNA 123

Query: 189 NGLSPKTTVADRGDPNDPRVRLKRDCVGIMAAFKLKKPFHHVVIVANTHLY--WDPEWAD 248
             LS                        +M AFK+ KPF+HVVI+A++HL       W D
Sbjct: 124 EELSV-----------------------VMVAFKILKPFNHVVIIASSHLKSGKPDRWDD 183

Query: 249 VKLAQAKYLLSRLARFKTLVAEKFECTPSVLLAGDFNSTPGDKVYQYLVSGNTSSGFSPE 308
           +KLAQ K L++ LA FK +++    C+PSV+LAGDFNS P   V++Y+ S N  S     
Sbjct: 184 LKLAQVKTLMTELASFKEIISALTNCSPSVILAGDFNSKP--YVHKYINSDNIPS----- 243

Query: 309 CLEELPLPLRSVYATILGSEPSFTNFTPGFTGTLDYIFLSPSDSIRPISLLELPESEWPE 368
                 + LRSVY    G EP FTN  PGF  TLDY+F + S+ I P+ LL+ P+     
Sbjct: 244 -----DIDLRSVYEFTKG-EPRFTNNVPGFAETLDYMFYTHSEIISPVKLLDSPDE---- 279

Query: 369 ILGGLPNYSYPSDHLPIGAEFEI 390
            +  LPN  +PSDHLPIG EFEI
Sbjct: 304 -VDFLPNEIHPSDHLPIGVEFEI 279