Spg016401 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016401 vs. NCBI nr
Match: XP_023536241.1 (probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita pepo subsp. pepo] >XP_023536242.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita pepo subsp. pepo] >XP_023536243.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.1 bits (1951), Expect = 2.4e-214
Identity = 388/517 (75.05%), Postives = 418/517 (80.85%), Query Frame = 0

Query: 117 MDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNSMNHEVQH 176
           MD R+RT  PSHTTPT+DLELDQ SLLPELSL G GAFS  S HSMVT SRNSMN +VQ 
Sbjct: 1   MDTRDRTRAPSHTTPTMDLELDQPSLLPELSLSGHGAFSRWSGHSMVTASRNSMNPDVQR 60

Query: 177 LPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRNGCIGIPA 236
           LPEHANGAILYG+N YNGSRIQRAQDIQVAT G+PCS+LTSP+GN+QFPSPR+G IG PA
Sbjct: 61  LPEHANGAILYGVNRYNGSRIQRAQDIQVATTGNPCSYLTSPYGNQQFPSPRSGLIGNPA 120

Query: 237 DACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAHSHLTRGDL 296
           DA ARNSHF+EVGFPYKRRFSE VP+NF GPN L SSESLNAPFNP+H H  SHLTRGDL
Sbjct: 121 DAYARNSHFMEVGFPYKRRFSEGVPVNFQGPNGLASSESLNAPFNPVHGHVPSHLTRGDL 180

Query: 297 MVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSAIYCI 356
           MVQ  QPASNAL LDQPVNFNF+DR AWTWN  PAGFAIHG                   
Sbjct: 181 MVQPFQPASNALRLDQPVNFNFDDRSAWTWNQTPAGFAIHG------------------- 240

Query: 357 FCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQPHFQV 416
                 DNGIRGSSESF+SGLHRYSEISGMSCSPNFQHPPSTMIQHSSH+IP  Q H   
Sbjct: 241 ------DNGIRGSSESFSSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHRIPLQQVH--- 300

Query: 417 RGQNISLHPQVAPAT--PRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYNPHQGCID 476
                 LHPQ A A   PR+ LSSAYGI+HQQF+ELEPRQT  LPFS R+AYNPHQGCID
Sbjct: 301 ------LHPQAAAAAAIPRVRLSSAYGIVHQQFMELEPRQTSDLPFSGRIAYNPHQGCID 360

Query: 477 PETMFRHRSSSQMRVLEEDEV--SVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEEL 536
           PETMFR RS+SQMRV EEDEV   V  A ASASDSN+ GDF+DAYRD+RLDIEHMSYEEL
Sbjct: 361 PETMFRRRSTSQMRVPEEDEVPFRVVQASASASDSNDIGDFADAYRDMRLDIEHMSYEEL 420

Query: 537 LALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICLDAINDG 596
           LALEERIGYV TGLSEETITSQLKTRISMPS+ +       SRNEE  SCTICLD +NDG
Sbjct: 421 LALEERIGYVRTGLSEETITSQLKTRISMPSSRE-------SRNEEPVSCTICLDTLNDG 475

Query: 597 KKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
            KIG+LDC HYYH+DCLKQWLL+KNVCP+CKSEALTR
Sbjct: 481 -KIGVLDCNHYYHADCLKQWLLVKNVCPICKSEALTR 475

BLAST of Spg016401 vs. NCBI nr
Match: XP_038897553.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X2 [Benincasa hispida])

HSP 1 Score: 753.1 bits (1943), Expect = 2.0e-213
Identity = 386/519 (74.37%), Postives = 417/519 (80.35%), Query Frame = 0

Query: 113 NCTFMDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNSMNH 172
           NCTFMDIRER  V SHT PT+DLELD+ SLLPELSLLGQGAFS  SSHSMVTTSRNSMN 
Sbjct: 17  NCTFMDIRERNRVLSHTPPTMDLELDRPSLLPELSLLGQGAFSQWSSHSMVTTSRNSMNP 76

Query: 173 EVQHLPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRNGCI 232
           +VQHLPEHANGA+LYG++ YNGSRIQ AQDIQV TA +PCS+LTSP+GN+ FPSPRNG I
Sbjct: 77  DVQHLPEHANGAVLYGVSQYNGSRIQCAQDIQVTTAANPCSYLTSPYGNQHFPSPRNGLI 136

Query: 233 GIPADACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAHSHLT 292
           G P D  ARNSHFIE GFPYKRRFSE  P++F GPNSL S ESLNAP      HAHSHL 
Sbjct: 137 GNPVDTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSLASFESLNAP------HAHSHLI 196

Query: 293 RGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSA 352
           RGDLMVQHLQP +NAL LDQPVNFN EDR AWTWN APAGF IHG               
Sbjct: 197 RGDLMVQHLQPTNNALWLDQPVNFNLEDRSAWTWNQAPAGFPIHG--------------- 256

Query: 353 IYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQP 412
                     D+GIRG SESFNSGLHRYSE+SG+S SPNFQHPPST+IQHSSH +P PQ 
Sbjct: 257 ----------DSGIRGFSESFNSGLHRYSEMSGIS-SPNFQHPPSTIIQHSSHPVPLPQA 316

Query: 413 HFQ-VRGQNISLHPQVAPATPRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYNPHQG 472
           H Q  RGQNISLHPQ A  TPR+PL+S YG MH QF ELE RQTR + F DR+ YNPHQ 
Sbjct: 317 HLQGQRGQNISLHPQAAATTPRVPLNSPYGNMH-QFPELELRQTRDITFGDRITYNPHQS 376

Query: 473 CIDPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEE 532
           C+DPET F+ R + QMRV EEDEV + GA ASASD N  GDF+DAYRD+RLDIEHMSYEE
Sbjct: 377 CVDPETTFQRRLTYQMRVPEEDEVPIRGARASASDPNGIGDFADAYRDMRLDIEHMSYEE 436

Query: 533 LLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLE-EAASRNEETNSCTICLDAIN 592
           LLALEERIGYVGTGLSEETITSQLKTRI+MPSA DVNLE  A SRNEETNSCTICLD I 
Sbjct: 437 LLALEERIGYVGTGLSEETITSQLKTRITMPSARDVNLEVGATSRNEETNSCTICLDVIG 496

Query: 593 DGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
           +G KIGILDC HYYH+DCLKQWLLIKNVCPVCKSEALTR
Sbjct: 497 EGTKIGILDCEHYYHADCLKQWLLIKNVCPVCKSEALTR 502

BLAST of Spg016401 vs. NCBI nr
Match: XP_038897552.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X1 [Benincasa hispida])

HSP 1 Score: 753.1 bits (1943), Expect = 2.0e-213
Identity = 386/519 (74.37%), Postives = 417/519 (80.35%), Query Frame = 0

Query: 113 NCTFMDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNSMNH 172
           NCTFMDIRER  V SHT PT+DLELD+ SLLPELSLLGQGAFS  SSHSMVTTSRNSMN 
Sbjct: 18  NCTFMDIRERNRVLSHTPPTMDLELDRPSLLPELSLLGQGAFSQWSSHSMVTTSRNSMNP 77

Query: 173 EVQHLPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRNGCI 232
           +VQHLPEHANGA+LYG++ YNGSRIQ AQDIQV TA +PCS+LTSP+GN+ FPSPRNG I
Sbjct: 78  DVQHLPEHANGAVLYGVSQYNGSRIQCAQDIQVTTAANPCSYLTSPYGNQHFPSPRNGLI 137

Query: 233 GIPADACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAHSHLT 292
           G P D  ARNSHFIE GFPYKRRFSE  P++F GPNSL S ESLNAP      HAHSHL 
Sbjct: 138 GNPVDTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSLASFESLNAP------HAHSHLI 197

Query: 293 RGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSA 352
           RGDLMVQHLQP +NAL LDQPVNFN EDR AWTWN APAGF IHG               
Sbjct: 198 RGDLMVQHLQPTNNALWLDQPVNFNLEDRSAWTWNQAPAGFPIHG--------------- 257

Query: 353 IYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQP 412
                     D+GIRG SESFNSGLHRYSE+SG+S SPNFQHPPST+IQHSSH +P PQ 
Sbjct: 258 ----------DSGIRGFSESFNSGLHRYSEMSGIS-SPNFQHPPSTIIQHSSHPVPLPQA 317

Query: 413 HFQ-VRGQNISLHPQVAPATPRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYNPHQG 472
           H Q  RGQNISLHPQ A  TPR+PL+S YG MH QF ELE RQTR + F DR+ YNPHQ 
Sbjct: 318 HLQGQRGQNISLHPQAAATTPRVPLNSPYGNMH-QFPELELRQTRDITFGDRITYNPHQS 377

Query: 473 CIDPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEE 532
           C+DPET F+ R + QMRV EEDEV + GA ASASD N  GDF+DAYRD+RLDIEHMSYEE
Sbjct: 378 CVDPETTFQRRLTYQMRVPEEDEVPIRGARASASDPNGIGDFADAYRDMRLDIEHMSYEE 437

Query: 533 LLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLE-EAASRNEETNSCTICLDAIN 592
           LLALEERIGYVGTGLSEETITSQLKTRI+MPSA DVNLE  A SRNEETNSCTICLD I 
Sbjct: 438 LLALEERIGYVGTGLSEETITSQLKTRITMPSARDVNLEVGATSRNEETNSCTICLDVIG 497

Query: 593 DGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
           +G KIGILDC HYYH+DCLKQWLLIKNVCPVCKSEALTR
Sbjct: 498 EGTKIGILDCEHYYHADCLKQWLLIKNVCPVCKSEALTR 503

BLAST of Spg016401 vs. NCBI nr
Match: XP_022976491.1 (probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita maxima] >XP_022976492.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita maxima] >XP_022976493.1 probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita maxima])

HSP 1 Score: 746.9 bits (1927), Expect = 1.4e-211
Identity = 381/516 (73.84%), Postives = 412/516 (79.84%), Query Frame = 0

Query: 117 MDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNSMNHEVQH 176
           MD R+RT  PSHTTP +DLELDQ S LPELSL G GAFS  S HSMVT SRNSMN +VQ 
Sbjct: 1   MDTRDRTRAPSHTTPIMDLELDQPSHLPELSLSGHGAFSRWSGHSMVTASRNSMNPDVQR 60

Query: 177 LPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRNGCIGIPA 236
           LPEHANGAILYG+N YN SRIQRAQDIQVAT G+PCS+LTSP+GN+QFPSPR+G IG PA
Sbjct: 61  LPEHANGAILYGVNRYNSSRIQRAQDIQVATTGNPCSYLTSPYGNQQFPSPRSGLIGNPA 120

Query: 237 DACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAHSHLTRGDL 296
           DA ARNSHF+EVGFPYKRRFSE VP+NF GPNSL SSESLN PFNP+H H  SHLTRGDL
Sbjct: 121 DAYARNSHFMEVGFPYKRRFSEGVPVNFQGPNSLASSESLNGPFNPVHGHVPSHLTRGDL 180

Query: 297 MVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSAIYCI 356
           MVQ  Q ASNAL LDQPVNFNF+DR AWTWN APAGFAIHG                   
Sbjct: 181 MVQPFQQASNALRLDQPVNFNFDDRSAWTWNQAPAGFAIHG------------------- 240

Query: 357 FCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQPHFQV 416
                 DNGIRGSSESF+SGLHRY EISGMSCSPNFQHPPSTMIQHSSH+IP  Q H   
Sbjct: 241 ------DNGIRGSSESFSSGLHRYGEISGMSCSPNFQHPPSTMIQHSSHRIPLQQVH--- 300

Query: 417 RGQNISLHPQVAPAT---PRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYNPHQGCI 476
                 LHPQ A A    PR+ LSSAYGI+HQQF+ELEPRQT  LPFS R+AYNPHQGCI
Sbjct: 301 ------LHPQAAAAAAAIPRVCLSSAYGIVHQQFMELEPRQTSDLPFSGRIAYNPHQGCI 360

Query: 477 DPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEELL 536
           DPETMFR RS+SQMRV EEDEV      ASAS+SN+ GDF+DAYRD+RLDIEHMSYEELL
Sbjct: 361 DPETMFRRRSTSQMRVPEEDEVPFRRVQASASNSNDTGDFADAYRDMRLDIEHMSYEELL 420

Query: 537 ALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICLDAINDGK 596
           ALEERIGYV TGLSEETITSQLKTRISMPS+ +       SRNEE  SCTICLD +NDG 
Sbjct: 421 ALEERIGYVRTGLSEETITSQLKTRISMPSSRE-------SRNEEPVSCTICLDTLNDG- 474

Query: 597 KIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
           KIG+LDC HYYH+DCLKQWLL+KNVCP+CKSEALTR
Sbjct: 481 KIGVLDCNHYYHADCLKQWLLVKNVCPICKSEALTR 474

BLAST of Spg016401 vs. NCBI nr
Match: XP_038897554.1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X3 [Benincasa hispida])

HSP 1 Score: 743.4 bits (1918), Expect = 1.6e-210
Identity = 382/515 (74.17%), Postives = 413/515 (80.19%), Query Frame = 0

Query: 117 MDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNSMNHEVQH 176
           MDIRER  V SHT PT+DLELD+ SLLPELSLLGQGAFS  SSHSMVTTSRNSMN +VQH
Sbjct: 1   MDIRERNRVLSHTPPTMDLELDRPSLLPELSLLGQGAFSQWSSHSMVTTSRNSMNPDVQH 60

Query: 177 LPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRNGCIGIPA 236
           LPEHANGA+LYG++ YNGSRIQ AQDIQV TA +PCS+LTSP+GN+ FPSPRNG IG P 
Sbjct: 61  LPEHANGAVLYGVSQYNGSRIQCAQDIQVTTAANPCSYLTSPYGNQHFPSPRNGLIGNPV 120

Query: 237 DACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAHSHLTRGDL 296
           D  ARNSHFIE GFPYKRRFSE  P++F GPNSL S ESLNAP      HAHSHL RGDL
Sbjct: 121 DTYARNSHFIEGGFPYKRRFSEGFPVSFQGPNSLASFESLNAP------HAHSHLIRGDL 180

Query: 297 MVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSAIYCI 356
           MVQHLQP +NAL LDQPVNFN EDR AWTWN APAGF IHG                   
Sbjct: 181 MVQHLQPTNNALWLDQPVNFNLEDRSAWTWNQAPAGFPIHG------------------- 240

Query: 357 FCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQPHFQ- 416
                 D+GIRG SESFNSGLHRYSE+SG+S SPNFQHPPST+IQHSSH +P PQ H Q 
Sbjct: 241 ------DSGIRGFSESFNSGLHRYSEMSGIS-SPNFQHPPSTIIQHSSHPVPLPQAHLQG 300

Query: 417 VRGQNISLHPQVAPATPRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYNPHQGCIDP 476
            RGQNISLHPQ A  TPR+PL+S YG MH QF ELE RQTR + F DR+ YNPHQ C+DP
Sbjct: 301 QRGQNISLHPQAAATTPRVPLNSPYGNMH-QFPELELRQTRDITFGDRITYNPHQSCVDP 360

Query: 477 ETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEELLAL 536
           ET F+ R + QMRV EEDEV + GA ASASD N  GDF+DAYRD+RLDIEHMSYEELLAL
Sbjct: 361 ETTFQRRLTYQMRVPEEDEVPIRGARASASDPNGIGDFADAYRDMRLDIEHMSYEELLAL 420

Query: 537 EERIGYVGTGLSEETITSQLKTRISMPSAMDVNLE-EAASRNEETNSCTICLDAINDGKK 596
           EERIGYVGTGLSEETITSQLKTRI+MPSA DVNLE  A SRNEETNSCTICLD I +G K
Sbjct: 421 EERIGYVGTGLSEETITSQLKTRITMPSARDVNLEVGATSRNEETNSCTICLDVIGEGTK 480

Query: 597 IGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
           IGILDC HYYH+DCLKQWLLIKNVCPVCKSEALTR
Sbjct: 481 IGILDCEHYYHADCLKQWLLIKNVCPVCKSEALTR 482

BLAST of Spg016401 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.0e-30
Identity = 140/549 (25.50%), Postives = 224/549 (40.80%), Query Frame = 0

Query: 129 TTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNSMNHEVQHLPEHANGAILYG 188
           T   IDLE +Q               SH  S S   T  +S +   QH       A   G
Sbjct: 9   TPQVIDLETEQ-------------GHSHIHSESFNRTGNDSSDQGAQHAVRGVGNATNIG 68

Query: 189 MNP----YNGSRIQRAQDIQ--------------VATAGSPCSFLTS----PFGNEQFPS 248
           ++     Y+       Q +                ++  +PC   TS        + F  
Sbjct: 69  LSDMRSYYDAGMNHPHQPVHNLPPNLGVDSGFVFPSSMYNPCMSTTSMNQYVSHTQSFGL 128

Query: 249 PRNGCIGIPADACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSH 308
           P N  +    D  +RN +  E    + +R + AV  ++H  N   SS S +A  NP H  
Sbjct: 129 PSNQVVLGSMDEGSRNENAGESARGFIKRKNAAVAGSYHCANGFASSSSSHASLNPTH-- 188

Query: 309 AHSHLTRGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLK 368
                       +   P+  +  L    ++N  +  + T  P+  G +I    F  +   
Sbjct: 189 ------------RPWDPSFESNVLPNTASYNPSEYHSQTSWPSMEGSSIPSNGFNLMGAH 248

Query: 369 KIDQSAIYCIFCGLH--------RDNGIRGSSESFNSGLHRYSEISGMSCSP-------- 428
                     F   H         +  I  S+     G+ ++  ++GM+ +P        
Sbjct: 249 PESAQHGNYAFPTSHISQCFQPTSNTWISQSANGIADGIPQWEYVNGMNNAPGRFSRSGM 308

Query: 429 ---------NFQHPPSTMIQHSSHQIPFPQPHFQVRGQNISLHPQV-APATPRIPLSSAY 488
                     +Q+ PST+ +     +P+   H  +   N+  H QV AP          +
Sbjct: 309 TETVNGSFREYQNGPSTLCR---GPLPYFHQHAGMHAHNLLDHTQVQAPYQQCHNNPVLH 368

Query: 489 GIMHQ-QFLELEPRQTRGLPFSDRMAYNPHQGCIDPETMFRHRSSSQMRVLEEDEVSVHG 548
           G+ H      L PR       S+R    PH   +       HR+   +R+L  +    H 
Sbjct: 369 GVNHSGNRFHLGPRIPVLFSNSERTFGPPHHPLL--ANPVNHRN---IRILPPE----HA 428

Query: 549 AHASASDSNERGDFSDAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTRI 608
                S   E  +  D +RD+RLDI+ M+YEELLALEE+IG V TGL++  I  +LKT +
Sbjct: 429 TIMDFSRLYEVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSL 488

Query: 609 SMPSAMDVNLEEAASRNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVC 629
            +P +  ++  +++  + E ++C IC +     + IG LDCGH YH DC+KQWL++KN+C
Sbjct: 489 FVPGSSCMS-NKSSESSMENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLC 517

BLAST of Spg016401 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 2.3e-26
Identity = 59/116 (50.86%), Postives = 77/116 (66.38%), Query Frame = 0

Query: 512 DFSDAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEE 571
           D  D +RD+RLDI++MSYEELLALEERIG V TGLSEE +T  LK R      ++ ++EE
Sbjct: 558 DIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEE 617

Query: 572 AASRNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEAL 628
                     C IC +   DG  +G LDCGH +H  C++QWL++KN CP+CK+ AL
Sbjct: 618 --------EPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTAL 665

BLAST of Spg016401 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 7.3e-25
Identity = 56/119 (47.06%), Postives = 79/119 (66.39%), Query Frame = 0

Query: 512 DFSDAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTR---ISMPSAMDVN 571
           D  D +R++RLD+++MSYEELLAL ERIG V TGLSEE I   +K      S PS++++ 
Sbjct: 591 DMHDRHREMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVEL- 650

Query: 572 LEEAASRNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEAL 628
                  ++    C IC +   +G  +G L CGH +H DC+KQW++IKN+CP+CK+EAL
Sbjct: 651 -------HQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTEAL 701

BLAST of Spg016401 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.8e-24
Identity = 52/113 (46.02%), Postives = 76/113 (67.26%), Query Frame = 0

Query: 515 DAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAAS 574
           D YRD+RLD+++MSYEELLALEERIG V TG++EETI+++LK R           +    
Sbjct: 579 DRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNRLKQR---------KYKSNTK 638

Query: 575 RNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEAL 628
             ++   C +C +   +G+ +G L+CGH +HS C+K+WL  KN+CP+CK+  L
Sbjct: 639 SPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNLCPICKTTGL 682

BLAST of Spg016401 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.0e-23
Identity = 52/117 (44.44%), Postives = 76/117 (64.96%), Query Frame = 0

Query: 512 DFSDAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEE 571
           +  D +RD+RLD+++MSYEELLAL ERIG V TGLSEE I   +K           +   
Sbjct: 557 EMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKVMK--------QHKHTSS 616

Query: 572 AASRNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALT 629
           AA  +++   C +C +   +G  +G L CGH +H+ C+KQWL++KN+CP+CK+ AL+
Sbjct: 617 AAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVALS 665

BLAST of Spg016401 vs. ExPASy TrEMBL
Match: A0A6J1IH15 (probable E3 ubiquitin-protein ligase ZFP1 OS=Cucurbita maxima OX=3661 GN=LOC111476874 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 6.9e-212
Identity = 381/516 (73.84%), Postives = 412/516 (79.84%), Query Frame = 0

Query: 117 MDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNSMNHEVQH 176
           MD R+RT  PSHTTP +DLELDQ S LPELSL G GAFS  S HSMVT SRNSMN +VQ 
Sbjct: 1   MDTRDRTRAPSHTTPIMDLELDQPSHLPELSLSGHGAFSRWSGHSMVTASRNSMNPDVQR 60

Query: 177 LPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRNGCIGIPA 236
           LPEHANGAILYG+N YN SRIQRAQDIQVAT G+PCS+LTSP+GN+QFPSPR+G IG PA
Sbjct: 61  LPEHANGAILYGVNRYNSSRIQRAQDIQVATTGNPCSYLTSPYGNQQFPSPRSGLIGNPA 120

Query: 237 DACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAHSHLTRGDL 296
           DA ARNSHF+EVGFPYKRRFSE VP+NF GPNSL SSESLN PFNP+H H  SHLTRGDL
Sbjct: 121 DAYARNSHFMEVGFPYKRRFSEGVPVNFQGPNSLASSESLNGPFNPVHGHVPSHLTRGDL 180

Query: 297 MVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSAIYCI 356
           MVQ  Q ASNAL LDQPVNFNF+DR AWTWN APAGFAIHG                   
Sbjct: 181 MVQPFQQASNALRLDQPVNFNFDDRSAWTWNQAPAGFAIHG------------------- 240

Query: 357 FCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQPHFQV 416
                 DNGIRGSSESF+SGLHRY EISGMSCSPNFQHPPSTMIQHSSH+IP  Q H   
Sbjct: 241 ------DNGIRGSSESFSSGLHRYGEISGMSCSPNFQHPPSTMIQHSSHRIPLQQVH--- 300

Query: 417 RGQNISLHPQVAPAT---PRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYNPHQGCI 476
                 LHPQ A A    PR+ LSSAYGI+HQQF+ELEPRQT  LPFS R+AYNPHQGCI
Sbjct: 301 ------LHPQAAAAAAAIPRVCLSSAYGIVHQQFMELEPRQTSDLPFSGRIAYNPHQGCI 360

Query: 477 DPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEELL 536
           DPETMFR RS+SQMRV EEDEV      ASAS+SN+ GDF+DAYRD+RLDIEHMSYEELL
Sbjct: 361 DPETMFRRRSTSQMRVPEEDEVPFRRVQASASNSNDTGDFADAYRDMRLDIEHMSYEELL 420

Query: 537 ALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICLDAINDGK 596
           ALEERIGYV TGLSEETITSQLKTRISMPS+ +       SRNEE  SCTICLD +NDG 
Sbjct: 421 ALEERIGYVRTGLSEETITSQLKTRISMPSSRE-------SRNEEPVSCTICLDTLNDG- 474

Query: 597 KIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
           KIG+LDC HYYH+DCLKQWLL+KNVCP+CKSEALTR
Sbjct: 481 KIGVLDCNHYYHADCLKQWLLVKNVCPICKSEALTR 474

BLAST of Spg016401 vs. ExPASy TrEMBL
Match: A0A6J1CD12 (probable E3 ubiquitin-protein ligase ZFP1 isoform X7 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 2.2e-205
Identity = 373/526 (70.91%), Postives = 412/526 (78.33%), Query Frame = 0

Query: 106 SVMLARDNCTFMDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTT 165
           S   +RD+CTFMDIR+RTL PSHTT  IDLELDQ+         GQG FS RS+HSMVT 
Sbjct: 21  SQFASRDSCTFMDIRKRTLAPSHTTSAIDLELDQQ---------GQGTFSQRSNHSMVTG 80

Query: 166 SRNSMNHEVQHLPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFP 225
            R+S N EVQH PEHANGAILY M+ Y+GS IQRAQD+ VATAG+PCS+LTSP+GNEQFP
Sbjct: 81  FRSSTNPEVQHQPEHANGAILYAMSQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFP 140

Query: 226 SPRNGCIGIPADACARNSHFI-EVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLH 285
           SPR+G +GIP+DA ARNSHFI EVG PYKRRF+E V +NF GPNS  SSESLNAPF+P+ 
Sbjct: 141 SPRSGLMGIPSDAYARNSHFIEEVGSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMF 200

Query: 286 SHAHSHLTRGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIP 345
            H  SHL RG+ MVQHLQPASNAL LDQ  N NFEDRGAW WN APAGF +HG       
Sbjct: 201 CHTRSHLARGEYMVQHLQPASNALWLDQRFNCNFEDRGAWNWNHAPAGFPVHG------- 260

Query: 346 LKKIDQSAIYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSS 405
                             DN IRG+SE+ NSGLHRYSEI GM+CSP+FQHPPST+IQH S
Sbjct: 261 ------------------DNAIRGASENSNSGLHRYSEIGGMNCSPSFQHPPSTLIQHPS 320

Query: 406 HQIPFPQPHFQ-VRGQNISLHPQVAPATPRIPLSSAYGIMHQQFLELEPRQTRGLPFSDR 465
           ++IP PQPH Q  RGQNI L+P VA  T R PLSSAYGIMHQQFLELEPRQTR LPF+DR
Sbjct: 321 YRIPLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEPRQTRDLPFNDR 380

Query: 466 MAYNPHQGCIDPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLD 525
             YNPH      ETMFR   +S+MRVL+E E  V GA ASAS +++  D  DAYRD+RLD
Sbjct: 381 RVYNPH------ETMFRRHLTSRMRVLQEGEAGVPGAQASAS-ASDSNDIGDAYRDMRLD 440

Query: 526 IEHMSYEELLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCT 585
           IEHMSYEELLALEE+IGYVGTGLSEE ITSQLKTRISMPSA D NLEEAASRNEETNSCT
Sbjct: 441 IEHMSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEETNSCT 500

Query: 586 ICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
           ICLD I DGKKIGILDCGHYYH+DCLKQWLLIKNVCPVCKSEALTR
Sbjct: 501 ICLDVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 505

BLAST of Spg016401 vs. ExPASy TrEMBL
Match: A0A6J1CCM1 (probable E3 ubiquitin-protein ligase ZFP1 isoform X6 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 3.7e-205
Identity = 372/522 (71.26%), Postives = 411/522 (78.74%), Query Frame = 0

Query: 110 ARDNCTFMDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNS 169
           +RD+CTFMDIR+RTL PSHTT  IDLELDQ+         GQG FS RS+HSMVT  R+S
Sbjct: 29  SRDSCTFMDIRKRTLAPSHTTSAIDLELDQQ---------GQGTFSQRSNHSMVTGFRSS 88

Query: 170 MNHEVQHLPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRN 229
            N EVQH PEHANGAILY M+ Y+GS IQRAQD+ VATAG+PCS+LTSP+GNEQFPSPR+
Sbjct: 89  TNPEVQHQPEHANGAILYAMSQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRS 148

Query: 230 GCIGIPADACARNSHFI-EVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAH 289
           G +GIP+DA ARNSHFI EVG PYKRRF+E V +NF GPNS  SSESLNAPF+P+  H  
Sbjct: 149 GLMGIPSDAYARNSHFIEEVGSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTR 208

Query: 290 SHLTRGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKI 349
           SHL RG+ MVQHLQPASNAL LDQ  N NFEDRGAW WN APAGF +HG           
Sbjct: 209 SHLARGEYMVQHLQPASNALWLDQRFNCNFEDRGAWNWNHAPAGFPVHG----------- 268

Query: 350 DQSAIYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIP 409
                         DN IRG+SE+ NSGLHRYSEI GM+CSP+FQHPPST+IQH S++IP
Sbjct: 269 --------------DNAIRGASENSNSGLHRYSEIGGMNCSPSFQHPPSTLIQHPSYRIP 328

Query: 410 FPQPHFQ-VRGQNISLHPQVAPATPRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYN 469
            PQPH Q  RGQNI L+P VA  T R PLSSAYGIMHQQFLELEPRQTR LPF+DR  YN
Sbjct: 329 LPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEPRQTRDLPFNDRRVYN 388

Query: 470 PHQGCIDPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHM 529
           PH      ETMFR   +S+MRVL+E E  V GA ASAS +++  D  DAYRD+RLDIEHM
Sbjct: 389 PH------ETMFRRHLTSRMRVLQEGEAGVPGAQASAS-ASDSNDIGDAYRDMRLDIEHM 448

Query: 530 SYEELLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICLD 589
           SYEELLALEE+IGYVGTGLSEE ITSQLKTRISMPSA D NLEEAASRNEETNSCTICLD
Sbjct: 449 SYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEETNSCTICLD 508

Query: 590 AINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
            I DGKKIGILDCGHYYH+DCLKQWLLIKNVCPVCKSEALTR
Sbjct: 509 VIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 509

BLAST of Spg016401 vs. ExPASy TrEMBL
Match: A0A6J1CFW4 (probable E3 ubiquitin-protein ligase ZFP1 isoform X5 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 3.7e-205
Identity = 372/523 (71.13%), Postives = 411/523 (78.59%), Query Frame = 0

Query: 109 LARDNCTFMDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRN 168
           + RD+CTFMDIR+RTL PSHTT  IDLELDQ+         GQG FS RS+HSMVT  R+
Sbjct: 31  MKRDSCTFMDIRKRTLAPSHTTSAIDLELDQQ---------GQGTFSQRSNHSMVTGFRS 90

Query: 169 SMNHEVQHLPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPR 228
           S N EVQH PEHANGAILY M+ Y+GS IQRAQD+ VATAG+PCS+LTSP+GNEQFPSPR
Sbjct: 91  STNPEVQHQPEHANGAILYAMSQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPR 150

Query: 229 NGCIGIPADACARNSHFI-EVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHA 288
           +G +GIP+DA ARNSHFI EVG PYKRRF+E V +NF GPNS  SSESLNAPF+P+  H 
Sbjct: 151 SGLMGIPSDAYARNSHFIEEVGSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHT 210

Query: 289 HSHLTRGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKK 348
            SHL RG+ MVQHLQPASNAL LDQ  N NFEDRGAW WN APAGF +HG          
Sbjct: 211 RSHLARGEYMVQHLQPASNALWLDQRFNCNFEDRGAWNWNHAPAGFPVHG---------- 270

Query: 349 IDQSAIYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQI 408
                          DN IRG+SE+ NSGLHRYSEI GM+CSP+FQHPPST+IQH S++I
Sbjct: 271 ---------------DNAIRGASENSNSGLHRYSEIGGMNCSPSFQHPPSTLIQHPSYRI 330

Query: 409 PFPQPHFQ-VRGQNISLHPQVAPATPRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAY 468
           P PQPH Q  RGQNI L+P VA  T R PLSSAYGIMHQQFLELEPRQTR LPF+DR  Y
Sbjct: 331 PLPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEPRQTRDLPFNDRRVY 390

Query: 469 NPHQGCIDPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEH 528
           NPH      ETMFR   +S+MRVL+E E  V GA ASAS +++  D  DAYRD+RLDIEH
Sbjct: 391 NPH------ETMFRRHLTSRMRVLQEGEAGVPGAQASAS-ASDSNDIGDAYRDMRLDIEH 450

Query: 529 MSYEELLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICL 588
           MSYEELLALEE+IGYVGTGLSEE ITSQLKTRISMPSA D NLEEAASRNEETNSCTICL
Sbjct: 451 MSYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEETNSCTICL 510

Query: 589 DAINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
           D I DGKKIGILDCGHYYH+DCLKQWLLIKNVCPVCKSEALTR
Sbjct: 511 DVIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 512

BLAST of Spg016401 vs. ExPASy TrEMBL
Match: A0A6J1CD07 (probable E3 ubiquitin-protein ligase ZFP1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111010433 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 3.7e-205
Identity = 372/522 (71.26%), Postives = 411/522 (78.74%), Query Frame = 0

Query: 110 ARDNCTFMDIRERTLVPSHTTPTIDLELDQRSLLPELSLLGQGAFSHRSSHSMVTTSRNS 169
           +RD+CTFMDIR+RTL PSHTT  IDLELDQ+         GQG FS RS+HSMVT  R+S
Sbjct: 61  SRDSCTFMDIRKRTLAPSHTTSAIDLELDQQ---------GQGTFSQRSNHSMVTGFRSS 120

Query: 170 MNHEVQHLPEHANGAILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFPSPRN 229
            N EVQH PEHANGAILY M+ Y+GS IQRAQD+ VATAG+PCS+LTSP+GNEQFPSPR+
Sbjct: 121 TNPEVQHQPEHANGAILYAMSQYSGSMIQRAQDMWVATAGNPCSYLTSPYGNEQFPSPRS 180

Query: 230 GCIGIPADACARNSHFI-EVGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNPLHSHAH 289
           G +GIP+DA ARNSHFI EVG PYKRRF+E V +NF GPNS  SSESLNAPF+P+  H  
Sbjct: 181 GLMGIPSDAYARNSHFIEEVGSPYKRRFAEPVLVNFQGPNSSASSESLNAPFDPMFCHTR 240

Query: 290 SHLTRGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKI 349
           SHL RG+ MVQHLQPASNAL LDQ  N NFEDRGAW WN APAGF +HG           
Sbjct: 241 SHLARGEYMVQHLQPASNALWLDQRFNCNFEDRGAWNWNHAPAGFPVHG----------- 300

Query: 350 DQSAIYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIP 409
                         DN IRG+SE+ NSGLHRYSEI GM+CSP+FQHPPST+IQH S++IP
Sbjct: 301 --------------DNAIRGASENSNSGLHRYSEIGGMNCSPSFQHPPSTLIQHPSYRIP 360

Query: 410 FPQPHFQ-VRGQNISLHPQVAPATPRIPLSSAYGIMHQQFLELEPRQTRGLPFSDRMAYN 469
            PQPH Q  RGQNI L+P VA  T R PLSSAYGIMHQQFLELEPRQTR LPF+DR  YN
Sbjct: 361 LPQPHLQGQRGQNICLNPPVAAVTQRFPLSSAYGIMHQQFLELEPRQTRDLPFNDRRVYN 420

Query: 470 PHQGCIDPETMFRHRSSSQMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHM 529
           PH      ETMFR   +S+MRVL+E E  V GA ASAS +++  D  DAYRD+RLDIEHM
Sbjct: 421 PH------ETMFRRHLTSRMRVLQEGEAGVPGAQASAS-ASDSNDIGDAYRDMRLDIEHM 480

Query: 530 SYEELLALEERIGYVGTGLSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICLD 589
           SYEELLALEE+IGYVGTGLSEE ITSQLKTRISMPSA D NLEEAASRNEETNSCTICLD
Sbjct: 481 SYEELLALEEQIGYVGTGLSEEIITSQLKTRISMPSARDANLEEAASRNEETNSCTICLD 540

Query: 590 AINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEALTR 630
            I DGKKIGILDCGHYYH+DCLKQWLLIKNVCPVCKSEALTR
Sbjct: 541 VIEDGKKIGILDCGHYYHADCLKQWLLIKNVCPVCKSEALTR 541

BLAST of Spg016401 vs. TAIR 10
Match: AT3G15070.1 (RING/U-box superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 3.6e-43
Identity = 158/502 (31.47%), Postives = 216/502 (43.03%), Query Frame = 0

Query: 174 VQHLPEHA------NGAILYGMNPYN-------GSRIQRAQDIQVATAG--SPCSFLTSP 233
           V HL  H+      N ++ YG   Y+       GS +  A +  V      +P SFL S 
Sbjct: 42  VPHLEAHSLQEPYDNNSMFYGPPQYHHQHASNLGSGMSTAPNFYVPYVNYEAPPSFLLSH 101

Query: 234 FGNEQFPSPRNGCIGIPADACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLN- 293
             N+         +G+ +    RN+HF++ GF  KR+ SE +P N   P +  S   LN 
Sbjct: 102 GSND-------AVVGVTSTEHERNAHFMDHGF--KRKSSEVIPGNSQYPVAPCSFPQLNT 161

Query: 294 ---APF--------------------------NPLHSHAHSHLTRGDLMVQHLQPASNAL 353
              APF                          +PL SH H++ ++G+    H  P   ++
Sbjct: 162 SETAPFSFPHFGTYPQPLDQRSVRNRAGAATMDPLLSHGHNNFSQGN-YAAHPFPPLGSI 221

Query: 354 SLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSAIYCIFCGLHRDNGIRG 413
             DQ  N N  D  +  W+ APA   +HG                          N   G
Sbjct: 222 WYDQHCNGNRSDGSSSLWSQAPAVPYMHG--------------------------NIATG 281

Query: 414 SSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQPHFQVRGQNISLHPQVA 473
           S ES N    RY E S                  SS     P P    R   IS HP   
Sbjct: 282 SIESGNVCFPRYHETS------------------SSRN---PTPSVYQRNHYISHHPVPP 341

Query: 474 P--ATPRIPLSSAYGIMH-QQFLELEPRQTRGLPFSDRMAYNPHQGCIDPETMFRHRSSS 533
           P    P +P +S    +H   +  +   Q+ G     R+   P +  + P  + RHR   
Sbjct: 342 PPIVYPHMPSASYAETLHPASYSHMGQVQSTGF----RINQYPGEDFV-PAAILRHRELP 401

Query: 534 QMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEELLALEERIGYVGTG 593
             R +  +E     A     D     ++ D ++D+RLDIE MSYEELLAL ++IG V TG
Sbjct: 402 HFRAMPANE----NAFWEVGDFYNAVNYVDHHQDMRLDIEDMSYEELLALSDQIGTVKTG 461

Query: 594 LSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICLDAINDGKKIGILDCGHYYH 628
           LS E +   LK R S      +NLEE  S + ET+SCTIC +   +  KI  LDC H YH
Sbjct: 462 LSSEDVKELLKRRTS----TRINLEEGPSTDLETDSCTICQENYKNEDKIATLDCMHKYH 473

BLAST of Spg016401 vs. TAIR 10
Match: AT3G15070.2 (RING/U-box superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 3.6e-43
Identity = 158/502 (31.47%), Postives = 216/502 (43.03%), Query Frame = 0

Query: 174 VQHLPEHA------NGAILYGMNPYN-------GSRIQRAQDIQVATAG--SPCSFLTSP 233
           V HL  H+      N ++ YG   Y+       GS +  A +  V      +P SFL S 
Sbjct: 42  VPHLEAHSLQEPYDNNSMFYGPPQYHHQHASNLGSGMSTAPNFYVPYVNYEAPPSFLLSH 101

Query: 234 FGNEQFPSPRNGCIGIPADACARNSHFIEVGFPYKRRFSEAVPLNFHGPNSLGSSESLN- 293
             N+         +G+ +    RN+HF++ GF  KR+ SE +P N   P +  S   LN 
Sbjct: 102 GSND-------AVVGVTSTEHERNAHFMDHGF--KRKSSEVIPGNSQYPVAPCSFPQLNT 161

Query: 294 ---APF--------------------------NPLHSHAHSHLTRGDLMVQHLQPASNAL 353
              APF                          +PL SH H++ ++G+    H  P   ++
Sbjct: 162 SETAPFSFPHFGTYPQPLDQRSVRNRAGAATMDPLLSHGHNNFSQGN-YAAHPFPPLGSI 221

Query: 354 SLDQPVNFNFEDRGAWTWNPAPAGFAIHGIFFLFIPLKKIDQSAIYCIFCGLHRDNGIRG 413
             DQ  N N  D  +  W+ APA   +HG                          N   G
Sbjct: 222 WYDQHCNGNRSDGSSSLWSQAPAVPYMHG--------------------------NIATG 281

Query: 414 SSESFNSGLHRYSEISGMSCSPNFQHPPSTMIQHSSHQIPFPQPHFQVRGQNISLHPQVA 473
           S ES N    RY E S                  SS     P P    R   IS HP   
Sbjct: 282 SIESGNVCFPRYHETS------------------SSRN---PTPSVYQRNHYISHHPVPP 341

Query: 474 P--ATPRIPLSSAYGIMH-QQFLELEPRQTRGLPFSDRMAYNPHQGCIDPETMFRHRSSS 533
           P    P +P +S    +H   +  +   Q+ G     R+   P +  + P  + RHR   
Sbjct: 342 PPIVYPHMPSASYAETLHPASYSHMGQVQSTGF----RINQYPGEDFV-PAAILRHRELP 401

Query: 534 QMRVLEEDEVSVHGAHASASDSNERGDFSDAYRDLRLDIEHMSYEELLALEERIGYVGTG 593
             R +  +E     A     D     ++ D ++D+RLDIE MSYEELLAL ++IG V TG
Sbjct: 402 HFRAMPANE----NAFWEVGDFYNAVNYVDHHQDMRLDIEDMSYEELLALSDQIGTVKTG 461

Query: 594 LSEETITSQLKTRISMPSAMDVNLEEAASRNEETNSCTICLDAINDGKKIGILDCGHYYH 628
           LS E +   LK R S      +NLEE  S + ET+SCTIC +   +  KI  LDC H YH
Sbjct: 462 LSSEDVKELLKRRTS----TRINLEEGPSTDLETDSCTICQENYKNEDKIATLDCMHKYH 473

BLAST of Spg016401 vs. TAIR 10
Match: AT1G53190.1 (RING/U-box superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 4.8e-40
Identity = 164/548 (29.93%), Postives = 242/548 (44.16%), Query Frame = 0

Query: 133 IDLELDQR----SLLPELSLLGQGAFSHRSSH----SMVTTSRNSMNHEVQHLPEHANGA 192
           +DLE++Q+    SL  E  +L  G+F  +  +    +MV    N   H +Q  P + N A
Sbjct: 8   VDLEMEQQQSQASLQAEPCIL-LGSFPQQPDNNNMPAMVAHVPNLEPHSLQD-PTYDNSA 67

Query: 193 ILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFP-SPRNGCIGI-PADACARN 252
           + YG+  Y+    QR           P     +P G  Q P S  +G +G+ P     RN
Sbjct: 68  MFYGLPQYHHHPHQRVP----TNFYVPYVAFQAPPG--QLPSSSSHGVVGVSPDHEYERN 127

Query: 253 SHFIE-VGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNP------------------- 312
           +HF++     YKR+ +E +P     P  L    +L APFN                    
Sbjct: 128 AHFMDHTRGTYKRKNAEGIP---GQPQYL---STLAAPFNTPETIAPFGGARNRPGAVTV 187

Query: 313 ---LHSHAHSHLTRGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIF 372
              L SHA ++  +G+    H  P   ++  DQ    +    G+ ++ P P         
Sbjct: 188 NTVLPSHAPNNFIQGNYAGHHPFPPPGSMWYDQ---HHGRSDGSPSFWPTPY-------- 247

Query: 373 FLFIPLKKIDQSAIYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTM 432
                               +H  N   GS ES +     +   S ++   ++       
Sbjct: 248 --------------------MHGSNIFAGSIESSSRNPTSFMYPSQLNPRDHY------- 307

Query: 433 IQHSSHQIPFPQPHFQVRGQNISLHPQVAPA----------TPRI-----PLSSAYGIMH 492
             +S H  P P P   +RGQN +L+P  A +          TP+      PL S  G  H
Sbjct: 308 --YSHHHHPAPPPVQGMRGQNATLYPHTASSASYRVPPGSFTPQNTMNSGPLGSEMGSSH 367

Query: 493 QQFLELEPRQTRGLPFSDRMAYNPHQ--GCIDPETMFRHRSS-SQMRVLEEDEVSV--HG 552
                + P Q  G        Y  HQ    +   T+ +HR    ++RV+ +DEV++   G
Sbjct: 368 -----MGPVQPTGF-----RIYQHHQRDDSVPVATLRQHRGGVPRLRVMPDDEVALLEFG 427

Query: 553 AHASASDSNERGDFSDAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTRI 612
                S +N      D +RD+RLDIE MSYEELLAL ERIG V TGL EE + + LKTR 
Sbjct: 428 DFLGGSGNNH----IDHHRDMRLDIEEMSYEELLALSERIGTVNTGLPEEDVKNHLKTRT 487

Query: 613 SMPSAMDVNLEEAASRNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVC 628
                 +       +++ ET  CTIC ++  + +KI  LDCGH YH++CL++WL++KNVC
Sbjct: 488 CSGINFEKESSSPRTKDLETEPCTICQESFKNEEKIATLDCGHEYHAECLEKWLIVKNVC 487

BLAST of Spg016401 vs. TAIR 10
Match: AT1G53190.2 (RING/U-box superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 4.8e-40
Identity = 164/548 (29.93%), Postives = 242/548 (44.16%), Query Frame = 0

Query: 133 IDLELDQR----SLLPELSLLGQGAFSHRSSH----SMVTTSRNSMNHEVQHLPEHANGA 192
           +DLE++Q+    SL  E  +L  G+F  +  +    +MV    N   H +Q  P + N A
Sbjct: 8   VDLEMEQQQSQASLQAEPCIL-LGSFPQQPDNNNMPAMVAHVPNLEPHSLQD-PTYDNSA 67

Query: 193 ILYGMNPYNGSRIQRAQDIQVATAGSPCSFLTSPFGNEQFP-SPRNGCIGI-PADACARN 252
           + YG+  Y+    QR           P     +P G  Q P S  +G +G+ P     RN
Sbjct: 68  MFYGLPQYHHHPHQRVP----TNFYVPYVAFQAPPG--QLPSSSSHGVVGVSPDHEYERN 127

Query: 253 SHFIE-VGFPYKRRFSEAVPLNFHGPNSLGSSESLNAPFNP------------------- 312
           +HF++     YKR+ +E +P     P  L    +L APFN                    
Sbjct: 128 AHFMDHTRGTYKRKNAEGIP---GQPQYL---STLAAPFNTPETIAPFGGARNRPGAVTV 187

Query: 313 ---LHSHAHSHLTRGDLMVQHLQPASNALSLDQPVNFNFEDRGAWTWNPAPAGFAIHGIF 372
              L SHA ++  +G+    H  P   ++  DQ    +    G+ ++ P P         
Sbjct: 188 NTVLPSHAPNNFIQGNYAGHHPFPPPGSMWYDQ---HHGRSDGSPSFWPTPY-------- 247

Query: 373 FLFIPLKKIDQSAIYCIFCGLHRDNGIRGSSESFNSGLHRYSEISGMSCSPNFQHPPSTM 432
                               +H  N   GS ES +     +   S ++   ++       
Sbjct: 248 --------------------MHGSNIFAGSIESSSRNPTSFMYPSQLNPRDHY------- 307

Query: 433 IQHSSHQIPFPQPHFQVRGQNISLHPQVAPA----------TPRI-----PLSSAYGIMH 492
             +S H  P P P   +RGQN +L+P  A +          TP+      PL S  G  H
Sbjct: 308 --YSHHHHPAPPPVQGMRGQNATLYPHTASSASYRVPPGSFTPQNTMNSGPLGSEMGSSH 367

Query: 493 QQFLELEPRQTRGLPFSDRMAYNPHQ--GCIDPETMFRHRSS-SQMRVLEEDEVSV--HG 552
                + P Q  G        Y  HQ    +   T+ +HR    ++RV+ +DEV++   G
Sbjct: 368 -----MGPVQPTGF-----RIYQHHQRDDSVPVATLRQHRGGVPRLRVMPDDEVALLEFG 427

Query: 553 AHASASDSNERGDFSDAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTRI 612
                S +N      D +RD+RLDIE MSYEELLAL ERIG V TGL EE + + LKTR 
Sbjct: 428 DFLGGSGNNH----IDHHRDMRLDIEEMSYEELLALSERIGTVNTGLPEEDVKNHLKTRT 487

Query: 613 SMPSAMDVNLEEAASRNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVC 628
                 +       +++ ET  CTIC ++  + +KI  LDCGH YH++CL++WL++KNVC
Sbjct: 488 CSGINFEKESSSPRTKDLETEPCTICQESFKNEEKIATLDCGHEYHAECLEKWLIVKNVC 487

BLAST of Spg016401 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 117.1 bits (292), Expect = 5.2e-26
Identity = 56/119 (47.06%), Postives = 79/119 (66.39%), Query Frame = 0

Query: 512 DFSDAYRDLRLDIEHMSYEELLALEERIGYVGTGLSEETITSQLKTR---ISMPSAMDVN 571
           D  D +R++RLD+++MSYEELLAL ERIG V TGLSEE I   +K      S PS++++ 
Sbjct: 591 DMHDRHREMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVEL- 650

Query: 572 LEEAASRNEETNSCTICLDAINDGKKIGILDCGHYYHSDCLKQWLLIKNVCPVCKSEAL 628
                  ++    C IC +   +G  +G L CGH +H DC+KQW++IKN+CP+CK+EAL
Sbjct: 651 -------HQNIEPCCICQEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTEAL 701