Spg016400 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016400
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Domain of unknown function (DUF303)
Location	scaffold9: 37963982 .. 37966783 (-)
RNA-Seq Expression	Spg016400
Synteny	Spg016400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGGTGAGTTGCTTGGCCAAATTTTGTCATCAACAGTTGACACCGTCTGTGGGGAAGAAGCTTGCTATTTTACATATACATCAAGGAAACAGAGAGAGGTTTTGGAGATGCAGAGGATTGCGCCGCGGTTCATGGGTGTGACCATACATGTAATAACCGAGCCTTACATACAGCGGTTTGTAGGATAGTTGCGTCTTTGGTTTTTAGCTGATGGGTTGAGTTAAGATCCGAGGAGTTATGTGAAGATCATTACTTGAGTGAGTTCACAGAATTTGTTTGGAATAGATTTGTGCATTATCCATGGATCGTGTAGTAATGCTTCTCTACTCTTTGGGTAAAGTTGCGTGAATAGATTGAATGATATTTGCTTGGACAGTGAGTTTGGGGCGATTCTTAAAAAAAGAAAATGTTTCTTAAGCATTTTAAAAGAAGGTTCTGAAGGATAATTTTAATCGGTTAAAAGCACTCCACTGTTTTAGAAAATTTTTATAATTCAATCTTTAGAGTTTGTTTGATAACTGGTTTTTTAAAATTAGGCTGCCTATAAGTTCTTTATTTTATTATCATGTTTTCAGAAACTGAGTTGTGAAAACTAAAACAAAGTAGCTTTTGTTTTTAGGATTTGGTTGATAATTACATTCTTAAATTTTAAATTAAAAGAAAAACTTAAACATTAACTTCGTTAAAAATGAAAATGTTGAATTTATAAAATGCTTAAAGGTTCTTTACTAACCTATTTCTAGAACTCTCTTTTGGATCACTCTGACGGAGTGGCGTTTGAGCCTAGGATTCTACATGGCAAGAAGATTGTTTAAGATTATGAGACGATAGCCAACAACAGCAAGGTTTGGCTGGCATAAAACTTGTATCTTGACCAAGAGGTTAAAGGTTCAATTTCCAATCCTACACGTTGTCAGAAAAAATATATATATATATGAGACGATAATTATTTCAAATGATTCGAGTAAGAAGATTAAAATGTAGGCATAAGTTAACATGTTCGATTGATTGATTGATTGATTGATTTATTATTATTATTATTTTATTCCAACAAGATTTGGGAATAGAGAGATTTGAATCCATTACCTCTTGGTACTCATGTCAGTTAAGCTATGCTTTTGTTAGCTCACGTTCGATAAGAGTTAACATCAATTAAACACCATATTTTTACCATTTTAGAAATTAATGGAGAATTGGTATATATTTTTACCTGTAAAATGTAATAATGTCTTTTTATCATTATTAGCGATTGACAAGCTCTTTTGAGATAGTTCTTGGGTAGGGGCGATCTCCTCCCCTTGGCCCCTAAGCTGTATTTGCCTTTGTGATTAATAGATATCTATTTATCGTTTCTTAAAAAAAAAAAAAAACTTTAAATGGAGTAACTATATATAAAGTTTGCTTCCAAACTTTTAAAATTATGCCTCTAATCTTATAGAATCGTGACAATTAAACTCTATTATTAGAGTTATAATTAGGGGCAGATCTTTGTAGGTTCCCTTTTAGAAGGAACTTGAGATTTTCCTCCATTTTAAAAAATACTGGCTCAAGTTTTTTATCCATACATCTATTTATAGAAAATTATAACAAATTAGGTGTGAACTAAGATGAAAAAGACCGACAAGCACTCACAATCCACAAATATTCACCCATTGACATTAACTTTGAAACATAAATAATACTAAAAATAGAATCAAAATGGAGTATAAAAGGTGAGGATCGCTATCGAAGAAAGACAGAGGCAAGGATCAGCCAACAAACAAAAGAGCAAGGGCGAGCAAAATTTCAAAGGAAGAGTAGTTGTGTCTGTGCAAATAGACACAGAGGAAAAGTTACAACTTTTTTTTTTTTAGACAAGACAAAGTAGCTTTCAATCCACTCAAACTTTTCTTCCGTGTCTACTTGCACAGACACAAGGGGTCAAAATGGCTTTGTTGAGACTATCTATCTTGTTATGTATGATGCTATTTGGTCCTTCCCTTTCAGGGGCTACTACTCCTAAGAACATATTCATCCTCGCTGGTCAAAGCAATATGGCTGGCCGAGGTGGAGTTGAGAATGATAAATCGGGAAAAAACCTCGTGTGGGATAGGTATGTCCCACCAATGTGTCAACCTGACCCATCCATCCTACGATTGAACCCTCAACTCCAATGGGAGATAGCACGAGAGCCTATCCATGCAGGGATCGACCTCCCCGACAAGACCCCTGGGATTGGTCCGGCAATGGCAATTGCTCGCCAGATACAAGCGAAAGTCGGGCAAAAGGCAGGTGTTGTGGGTTTAGTTCCTTGTGCTAAAGGTGGCACTTTAATCAAACAATGGATGAAAAATCCTAGCAATCCTAATGCAACTTTTTACCAAAATTTCATCGAACGAATCAAAGCGTCAGATAAAGAAGGTGGGGTGGTGCGTGCTCTTTTCTGGCACCTAGGGGAAAGCGATGCGGCTATGAGTGACACTGCTCATAAATACAAAGATAACTTAAAGAAGTTCTTCACTGACGTCCGCAATGATATAAAACCTAGATTTTTACCCATCATTATTGTTAAAGCAACCCTCTATGATTTTTTTATGAAGCATGATACTCATGATTTGCCAGCAGTGAGGGCGGCACAAGAAGCACTCCAACACGAGTTGCCGGACGTGGTGACCATCGACTCCTGGAAATTGCCTATAAACTTGAACACTCATGAAGGTTTTAACATGGATCACGGTCATTTTAATACTGCCACTGAGATTGCTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCACTATGGTCATTTACTCTAA

mRNA sequence

ATGTCAGGTGAGTTGCTTGGCCAAATTTTGTCATCAACAGTTGACACCGTCTGTGGGGAAGAAGCTTGCTATTTTACATATACATCAAGGAAACAGAGAGAGGTTTTGGAGATGCAGAGGATTGCGCCGCGGTTCATGGGTGTGACCATACATGTAATAACCGAGCCTTACATACAGCGATTGAATGATATTTGCTTGGACAGGGCTACTACTCCTAAGAACATATTCATCCTCGCTGGTCAAAGCAATATGGCTGGCCGAGGTGGAGTTGAGAATGATAAATCGGGAAAAAACCTCGTGTGGGATAGGTATGTCCCACCAATGTGTCAACCTGACCCATCCATCCTACGATTGAACCCTCAACTCCAATGGGAGATAGCACGAGAGCCTATCCATGCAGGGATCGACCTCCCCGACAAGACCCCTGGGATTGGTCCGGCAATGGCAATTGCTCGCCAGATACAAGCGAAAGTCGGGCAAAAGGCAGGTGTTGTGGGTTTAGTTCCTTGTGCTAAAGGTGGCACTTTAATCAAACAATGGATGAAAAATCCTAGCAATCCTAATGCAACTTTTTACCAAAATTTCATCGAACGAATCAAAGCGTCAGATAAAGAAGGTGGGGTGGTGCGTGCTCTTTTCTGGCACCTAGGGGAAAGCGATGCGGCTATGAGTGACACTGCTCATAAATACAAAGATAACTTAAAGAAGTTCTTCACTGACGTCCGCAATGATATAAAACCTAGATTTTTACCCATCATTATTGTTAAAGCAACCCTCTATGATTTTTTTATGAAGCATGATACTCATGATTTGCCAGCAGTGAGGGCGGCACAAGAAGCACTCCAACACGAGTTGCCGGACGTGGTGACCATCGACTCCTGGAAATTGCCTATAAACTTGAACACTCATGAAGGTTTTAACATGGATCACGGTCATTTTAATACTGCCACTGAGATTGCTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCACTATGGTCATTTACTCTAA

Coding sequence (CDS)

ATGTCAGGTGAGTTGCTTGGCCAAATTTTGTCATCAACAGTTGACACCGTCTGTGGGGAAGAAGCTTGCTATTTTACATATACATCAAGGAAACAGAGAGAGGTTTTGGAGATGCAGAGGATTGCGCCGCGGTTCATGGGTGTGACCATACATGTAATAACCGAGCCTTACATACAGCGATTGAATGATATTTGCTTGGACAGGGCTACTACTCCTAAGAACATATTCATCCTCGCTGGTCAAAGCAATATGGCTGGCCGAGGTGGAGTTGAGAATGATAAATCGGGAAAAAACCTCGTGTGGGATAGGTATGTCCCACCAATGTGTCAACCTGACCCATCCATCCTACGATTGAACCCTCAACTCCAATGGGAGATAGCACGAGAGCCTATCCATGCAGGGATCGACCTCCCCGACAAGACCCCTGGGATTGGTCCGGCAATGGCAATTGCTCGCCAGATACAAGCGAAAGTCGGGCAAAAGGCAGGTGTTGTGGGTTTAGTTCCTTGTGCTAAAGGTGGCACTTTAATCAAACAATGGATGAAAAATCCTAGCAATCCTAATGCAACTTTTTACCAAAATTTCATCGAACGAATCAAAGCGTCAGATAAAGAAGGTGGGGTGGTGCGTGCTCTTTTCTGGCACCTAGGGGAAAGCGATGCGGCTATGAGTGACACTGCTCATAAATACAAAGATAACTTAAAGAAGTTCTTCACTGACGTCCGCAATGATATAAAACCTAGATTTTTACCCATCATTATTGTTAAAGCAACCCTCTATGATTTTTTTATGAAGCATGATACTCATGATTTGCCAGCAGTGAGGGCGGCACAAGAAGCACTCCAACACGAGTTGCCGGACGTGGTGACCATCGACTCCTGGAAATTGCCTATAAACTTGAACACTCATGAAGGTTTTAACATGGATCACGGTCATTTTAATACTGCCACTGAGATTGCTTTGGGTAAATGGTTGGCTGATACCTACCTCTCCCACTATGGTCATTTACTCTAA

Protein sequence

MSGELLGQILSSTVDTVCGEEACYFTYTSRKQREVLEMQRIAPRFMGVTIHVITEPYIQRLNDICLDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWEIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPSNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDIKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEGFNMDHGHFNTATEIALGKWLADTYLSHYGHLL

Homology

[Close]

BLAST of Spg016400 vs. NCBI nr
Match: XP_023002177.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002871.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 453.8 bits (1166), Expect = 1.3e-123
Identity = 212/272 (77.94%), Postives = 240/272 (88.24%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L  AT+P NIFILAGQSNMAGRGGVEN++ GK L WD  VP  CQ DPSILRLNP  QWE
Sbjct: 19  LSWATSPTNIFILAGQSNMAGRGGVENNQKGK-LEWDGKVPLECQSDPSILRLNPARQWE 78

Query: 126 IAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPS 185
           IA+EP+H GID+  KTPGIGP +  A Q +AK GQKAG+VGLVPCA+GGTLI+QW+KNPS
Sbjct: 79  IAQEPLHLGIDI-GKTPGIGPGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWIKNPS 138

Query: 186 NPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDI 245
           NP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAMSDTAH+YKDNLKKF TD+RNDI
Sbjct: 139 NPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFITDIRNDI 198

Query: 246 KPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEG 305
           KPRFLP+IIVK ++YDFFMKHDTHDLPAVRAA++A+Q ELPD++TIDSW+LPIN  T EG
Sbjct: 199 KPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDIITIDSWELPINFTTFEG 258

Query: 306 FNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           F +DHGHFNTATEIALGKWLADTYL+HY HLL
Sbjct: 259 FCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

[Close]

BLAST of Spg016400 vs. NCBI nr
Match: XP_038886575.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 451.8 bits (1161), Expect = 5.1e-123
Identity = 213/272 (78.31%), Postives = 239/272 (87.87%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L RA +PKNIFILAGQSNMAGRGGVEN++ GK L W+R +PP CQ D SILRLNP LQWE
Sbjct: 19  LSRAASPKNIFILAGQSNMAGRGGVENNQVGK-LEWNRLIPPECQSDTSILRLNPALQWE 78

Query: 126 IAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPS 185
           +AREP+H GID+ +KT GIGP M  A Q+ AKVG KAG+VGLVPCAKGGT+I+QW+KNPS
Sbjct: 79  MAREPLHEGIDI-NKTVGIGPGMPFAHQLLAKVGPKAGIVGLVPCAKGGTIIEQWIKNPS 138

Query: 186 NPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDI 245
           NP+ATFY++FIERIKASDKEGGVVRALFW  GESDAAM+DTA +YKDNLK FFTD+RNDI
Sbjct: 139 NPDATFYKSFIERIKASDKEGGVVRALFWFQGESDAAMNDTASRYKDNLKNFFTDIRNDI 198

Query: 246 KPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEG 305
           KPRFLPII+VK  LYDF MKHDTHDLP VRAAQ+A+  ELPDVVTID+ KLPIN++THEG
Sbjct: 199 KPRFLPIILVKIALYDFMMKHDTHDLPVVRAAQDAVSKELPDVVTIDALKLPINVDTHEG 258

Query: 306 FNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           FN DHGHFNT TEI LGKWLADTYLSHYGHLL
Sbjct: 259 FNQDHGHFNTTTEITLGKWLADTYLSHYGHLL 288

[Close]

BLAST of Spg016400 vs. NCBI nr
Match: XP_038886442.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 448.7 bits (1153), Expect = 4.3e-122
Identity = 210/272 (77.21%), Postives = 237/272 (87.13%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L RA +P NIFILAGQSNMAGRGGVEN++  + L WD  +PP CQ DPSILRLNP LQWE
Sbjct: 19  LSRAASPNNIFILAGQSNMAGRGGVENNQV-RELEWDGLIPPECQSDPSILRLNPALQWE 78

Query: 126 IAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPS 185
           IAREP+H GID+ +KT GIGP M  A Q+  KVG +AG VGLVPCA+GGT+I+QW+KNPS
Sbjct: 79  IAREPLHEGIDI-NKTVGIGPGMPFAHQLLTKVGPRAGTVGLVPCARGGTIIEQWIKNPS 138

Query: 186 NPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDI 245
           NP+ATFY+NFIERIKASDKEGGVVRALFW  GESDAAMSDTA++YKDNLK FFTD+RNDI
Sbjct: 139 NPDATFYKNFIERIKASDKEGGVVRALFWFQGESDAAMSDTANRYKDNLKNFFTDIRNDI 198

Query: 246 KPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEG 305
           KPRFLPII+VK  LYDF MKHDTHDLPAVRAAQ+A+  ELPD+VTID+ KLPIN++THEG
Sbjct: 199 KPRFLPIILVKIALYDFMMKHDTHDLPAVRAAQDAVSKELPDIVTIDALKLPINVDTHEG 258

Query: 306 FNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           FN DHGHFNT T+I LGKWLADTYLSHYGHLL
Sbjct: 259 FNQDHGHFNTTTQITLGKWLADTYLSHYGHLL 288

[Close]

BLAST of Spg016400 vs. NCBI nr
Match: XP_022973316.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_022973317.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 446.4 bits (1147), Expect = 2.1e-121
Identity = 208/273 (76.19%), Postives = 238/273 (87.18%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L  AT+P NIFILAGQSNMAGRGGVE   +G+ LVWD  VP  CQ DPSILR NP+ QWE
Sbjct: 19  LSGATSPTNIFILAGQSNMAGRGGVEKTPTGE-LVWDGKVPSECQSDPSILRFNPERQWE 78

Query: 126 IAREPIHAGIDL-PDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNP 185
           IA EP+H GID+   KTPGIGP +  A Q++ K GQKAG+VGLVPCA+GGTLI+QW+KNP
Sbjct: 79  IAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNP 138

Query: 186 SNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRND 245
           SNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA +YKDNLKKF TD+RND
Sbjct: 139 SNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRND 198

Query: 246 IKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHE 305
           IKPRFLP+IIVK  LYDFFMKHDTH+LPAVRAA++A+Q ELPD++TIDSW+LP+NL T E
Sbjct: 199 IKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTTFE 258

Query: 306 GFNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           GF+ DHGHFNTATEIALGKWLADTYL+HYGHLL
Sbjct: 259 GFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

[Close]

BLAST of Spg016400 vs. NCBI nr
Match: KAE8646868.1 (hypothetical protein Csa_020851 [Cucumis sativus])

HSP 1 Score: 444.1 bits (1141), Expect = 1.1e-120
Identity = 209/273 (76.56%), Postives = 235/273 (86.08%), Query Frame = 0


Query: 65  CLDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQW 124
           CL  A +PKNIFI AGQSNMAGRGGVEN+  G NL+WD  VPP CQ +PSILRLNP  QW
Sbjct: 18  CLSGAISPKNIFIFAGQSNMAGRGGVENNNKG-NLMWDGLVPPECQSEPSILRLNPDRQW 77

Query: 125 EIAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNP 184
           EIAREP+H GID+ ++TPGIGP M  A ++ AKVG  AG VGLVPCA+GGTLI QW+KNP
Sbjct: 78  EIAREPLHLGIDI-NRTPGIGPGMPFAHELLAKVGPNAGAVGLVPCARGGTLIGQWVKNP 137

Query: 185 SNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRND 244
           SNP+ATFYQNFIERIKASDK+GGVVRALFW  GESDAAM+DTA +YKDNLKKFFTD+RND
Sbjct: 138 SNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIRYKDNLKKFFTDIRND 197

Query: 245 IKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHE 304
           IKPRFLPII+VK  LYDF M+HDTH+LPAVR AQ+A+  ELPDVV IDS +LPINL T+E
Sbjct: 198 IKPRFLPIIVVKIALYDFMMQHDTHNLPAVREAQDAVSKELPDVVAIDSLELPINLTTNE 257

Query: 305 GFNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           GFN+DHGHFNT TEI LGKWLA+TYLSHYGHLL
Sbjct: 258 GFNLDHGHFNTTTEITLGKWLANTYLSHYGHLL 288

[Close]

BLAST of Spg016400 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 2.2e-44
Identity = 101/262 (38.55%), Postives = 145/262 (55.34%), Query Frame = 0


Query: 72  PKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWEIAREPI 131
           P  IFIL+GQSNMAGRGGV  D      VWD+ +PP C P+ SILRL+  L+WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 132 HAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPSNPNATF 191
           H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT IK+W +      +  
Sbjct: 81  HVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHL 140

Query: 192 YQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDIKPRFLP 251
           Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+D+    LP
Sbjct: 141 YERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLP 200

Query: 252 IIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEGFNMDHG 311
           II V       +       +  VR AQ  L  +L +VV +D+  LP+          D+ 
Sbjct: 201 IIQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL--------KSDNL 259

Query: 312 HFNTATEIALGKWLADTYLSHY 334
           H  T  ++ LG  LA  YLS++
Sbjct: 261 HLTTEAQVQLGLSLAQAYLSNF 259

[Close]

BLAST of Spg016400 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 6.5e-124
Identity = 212/272 (77.94%), Postives = 240/272 (88.24%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L  AT+P NIFILAGQSNMAGRGGVEN++ GK L WD  VP  CQ DPSILRLNP  QWE
Sbjct: 19  LSWATSPTNIFILAGQSNMAGRGGVENNQKGK-LEWDGKVPLECQSDPSILRLNPARQWE 78

Query: 126 IAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPS 185
           IA+EP+H GID+  KTPGIGP +  A Q +AK GQKAG+VGLVPCA+GGTLI+QW+KNPS
Sbjct: 79  IAQEPLHLGIDI-GKTPGIGPGIPFAHQFKAKAGQKAGIVGLVPCARGGTLIEQWIKNPS 138

Query: 186 NPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDI 245
           NP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAMSDTAH+YKDNLKKF TD+RNDI
Sbjct: 139 NPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHRYKDNLKKFITDIRNDI 198

Query: 246 KPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEG 305
           KPRFLP+IIVK ++YDFFMKHDTHDLPAVRAA++A+Q ELPD++TIDSW+LPIN  T EG
Sbjct: 199 KPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDIITIDSWELPINFTTFEG 258

Query: 306 FNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           F +DHGHFNTATEIALGKWLADTYL+HY HLL
Sbjct: 259 FCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

[Close]

BLAST of Spg016400 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 1.0e-121
Identity = 208/273 (76.19%), Postives = 238/273 (87.18%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L  AT+P NIFILAGQSNMAGRGGVE   +G+ LVWD  VP  CQ DPSILR NP+ QWE
Sbjct: 19  LSGATSPTNIFILAGQSNMAGRGGVEKTPTGE-LVWDGKVPSECQSDPSILRFNPERQWE 78

Query: 126 IAREPIHAGIDL-PDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNP 185
           IA EP+H GID+   KTPGIGP +  A Q++ K GQKAG+VGLVPCA+GGTLI+QW+KNP
Sbjct: 79  IAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNP 138

Query: 186 SNPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRND 245
           SNP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA +YKDNLKKF TD+RND
Sbjct: 139 SNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRND 198

Query: 246 IKPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHE 305
           IKPRFLP+IIVK  LYDFFMKHDTH+LPAVRAA++A+Q ELPD++TIDSW+LP+NL T E
Sbjct: 199 IKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTTFE 258

Query: 306 GFNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           GF+ DHGHFNTATEIALGKWLADTYL+HYGHLL
Sbjct: 259 GFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

[Close]

BLAST of Spg016400 vs. ExPASy TrEMBL
Match: A0A5A7VGP3 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold37G00180 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 6.3e-119
Identity = 204/272 (75.00%), Postives = 232/272 (85.29%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L  A +PKNIFILAGQSNMAGRGGVE D+SG NLVWDR VPP C+P PSILRLNP+ +WE
Sbjct: 25  LSGAASPKNIFILAGQSNMAGRGGVEKDQSG-NLVWDRLVPPECEPQPSILRLNPEREWE 84

Query: 126 IAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPS 185
            AREP+H GID+ ++T GIGP M  A  + AK G  AGVVGLVPCA+GGTLI+QW+KNPS
Sbjct: 85  TAREPLHVGIDI-NRTAGIGPGMPFAHHLLAKAGPNAGVVGLVPCARGGTLIEQWIKNPS 144

Query: 186 NPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDI 245
           NPNATFY+NFIERIKASDK+GGVVRALFW  GESDAAMSDTAH+YKDNLK+FFTD+RNDI
Sbjct: 145 NPNATFYKNFIERIKASDKDGGVVRALFWFQGESDAAMSDTAHRYKDNLKQFFTDIRNDI 204

Query: 246 KPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEG 305
           KPRFLPII+ K  +YD FMKHDTHDL AVRAAQE +  ELPD++TID+ +LPIN  T+ G
Sbjct: 205 KPRFLPIILAKIAVYDPFMKHDTHDLAAVRAAQEEVSKELPDILTIDALQLPINFTTNAG 264

Query: 306 FNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           FN+DH HFNT TEI +GKW ADTYLSHYGHLL
Sbjct: 265 FNLDHAHFNTNTEIVVGKWFADTYLSHYGHLL 294

[Close]

BLAST of Spg016400 vs. ExPASy TrEMBL
Match: A0A6J1DUH7 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111024143 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.8e-118
Identity = 209/272 (76.84%), Postives = 239/272 (87.87%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L RAT+PKNIFILAGQSNMAGRGGVEND+ G +LVWD YVPP  QPDPSILRLNP+ QWE
Sbjct: 19  LSRATSPKNIFILAGQSNMAGRGGVENDRPG-HLVWDGYVPPEAQPDPSILRLNPERQWE 78

Query: 126 IAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPS 185
           +AREP+H GID+ +KT G+GPA+A ARQ+QAK G K G VGLVPCA+GGTLI+QW+KNPS
Sbjct: 79  VAREPVHEGIDI-NKTVGVGPAIAFARQLQAKGGSKVGSVGLVPCARGGTLIEQWVKNPS 138

Query: 186 NPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDI 245
           N +ATFY+NFIERI+ASD+EGGVVRALFW  GESDAA  DTA++YK+NLKKFFTD+RNDI
Sbjct: 139 NTSATFYKNFIERIQASDREGGVVRALFWLQGESDAASRDTANRYKNNLKKFFTDIRNDI 198

Query: 246 KPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEG 305
           KPRFLPII+VK  +YD FMKHDTHDLPAVRAA++A+Q ELP+VVTIDS KL +N  T EG
Sbjct: 199 KPRFLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVVTIDSLKL-VNTTTVEG 258

Query: 306 FNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           FN+D GHFN  TEIALGKWLADTYLS YGHLL
Sbjct: 259 FNLDRGHFNIKTEIALGKWLADTYLSQYGHLL 287

[Close]

BLAST of Spg016400 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 3.1e-118
Identity = 204/272 (75.00%), Postives = 235/272 (86.40%), Query Frame = 0


Query: 66  LDRATTPKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWE 125
           L  AT+P NIFILAGQSNMAGRGGVE  ++GK LVWD  VP  CQ DPSILRLNP+ QWE
Sbjct: 19  LSGATSPTNIFILAGQSNMAGRGGVEKIQNGK-LVWDGKVPLECQFDPSILRLNPERQWE 78

Query: 126 IAREPIHAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPS 185
           IA EP+H GID+   TPGIGP +  A Q + K GQKAG+VGLVPCA+GGTLI+QW+KNPS
Sbjct: 79  IAHEPLHLGIDI-SHTPGIGPGIPFAHQFKEKAGQKAGIVGLVPCARGGTLIEQWIKNPS 138

Query: 186 NPNATFYQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDI 245
           NP+ATFYQNFIERIK S+KEGGVVRALFW+ GESDAAM+DTA +YKDNLKKF TD+RNDI
Sbjct: 139 NPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDI 198

Query: 246 KPRFLPIIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEG 305
           KPRFLP+IIVK +LYDFFMKHDTH+LPAVRAA++A+Q ELPD++TIDS +LP+N  T EG
Sbjct: 199 KPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSRELPVNFTTFEG 258

Query: 306 FNMDHGHFNTATEIALGKWLADTYLSHYGHLL 338
           F+ DHGHFNT TEI LGKWLA+TYL+HYGHLL
Sbjct: 259 FSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

[Close]

BLAST of Spg016400 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 181.8 bits (460), Expect = 9.1e-46
Identity = 108/261 (41.38%), Postives = 153/261 (58.62%), Query Frame = 0


Query: 74  NIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWEIAREPIHA 133
           +IFILAGQSNMAGRGGV ND +    VWD  +PP C+ +PSILRL  +L+W+ A+EP+H 
Sbjct: 30  SIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHV 89

Query: 134 GIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPSNPNATFYQ 193
            ID+ +KT G+GP M  A ++  + GQ    VGLVPC+ GGT + QW K         Y+
Sbjct: 90  DIDI-NKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQK-----GEFLYE 149

Query: 194 NFIERIKA--SDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDIKPRFLP 253
             ++R KA  +   GG  RA+ W+ GESD      A  YK  L KFF+D+RND++   LP
Sbjct: 150 ETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLP 209

Query: 254 II-IVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEGFNMDH 313
           II +  AT    +       L AVR AQ  L+ +L +V  +D+  LP+          D 
Sbjct: 210 IIQVALATGAGPY-------LDAVRKAQ--LKTDLENVYCVDARGLPL--------EPDG 263

Query: 314 GHFNTATEIALGKWLADTYLS 332
            H  T++++ LG  +A+++L+
Sbjct: 270 LHLTTSSQVQLGHMIAESFLA 263

[Close]

BLAST of Spg016400 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-45
Identity = 101/262 (38.55%), Postives = 145/262 (55.34%), Query Frame = 0


Query: 72  PKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWEIAREPI 131
           P  IFIL+GQSNMAGRGGV  D      VWD+ +PP C P+ SILRL+  L+WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 132 HAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPSNPNATF 191
           H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT IK+W +      +  
Sbjct: 81  HVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHL 140

Query: 192 YQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDIKPRFLP 251
           Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+D+    LP
Sbjct: 141 YERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLP 200

Query: 252 IIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEGFNMDHG 311
           II V       +       +  VR AQ  L  +L +VV +D+  LP+          D+ 
Sbjct: 201 IIQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL--------KSDNL 259

Query: 312 HFNTATEIALGKWLADTYLSHY 334
           H  T  ++ LG  LA  YLS++
Sbjct: 261 HLTTEAQVQLGLSLAQAYLSNF 259

[Close]

BLAST of Spg016400 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-45
Identity = 101/262 (38.55%), Postives = 145/262 (55.34%), Query Frame = 0


Query: 72  PKNIFILAGQSNMAGRGGVENDKSGKNLVWDRYVPPMCQPDPSILRLNPQLQWEIAREPI 131
           P  IFIL+GQSNMAGRGGV  D      VWD+ +PP C P+ SILRL+  L+WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 132 HAGIDLPDKTPGIGPAMAIARQIQAKVGQKAGVVGLVPCAKGGTLIKQWMKNPSNPNATF 191
           H  ID   K  G+GP MA A  ++ ++   + V+GLVPCA GGT IK+W +      +  
Sbjct: 81  HVDID-TGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHL 140

Query: 192 YQNFIERIKASDKEGGVVRALFWHLGESDAAMSDTAHKYKDNLKKFFTDVRNDIKPRFLP 251
           Y+  ++R + S K GG ++A+ W+ GESD      A  Y +N+ +   ++R+D+    LP
Sbjct: 141 YERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLP 200

Query: 252 IIIVKATLYDFFMKHDTHDLPAVRAAQEALQHELPDVVTIDSWKLPINLNTHEGFNMDHG 311
           II V       +       +  VR AQ  L  +L +VV +D+  LP+          D+ 
Sbjct: 201 IIQVAIASGGGY-------IDKVREAQLGL--KLSNVVCVDAKGLPL--------KSDNL 259

Query: 312 HFNTATEIALGKWLADTYLSHY 334
           H  T  ++ LG  LA  YLS++
Sbjct: 261 HLTTEAQVQLGLSLAQAYLSNF 259

The following BLAST results are available for this feature:

BLAST of Spg016400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002177.1	1.3e-123	77.94	probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 prob...	[more]
XP_038886575.1	5.1e-123	78.31	probable carbohydrate esterase At4g34215 [Benincasa hispida]	[more]
XP_038886442.1	4.3e-122	77.21	probable carbohydrate esterase At4g34215 [Benincasa hispida]	[more]
XP_022973316.1	2.1e-121	76.19	probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_022973317.1 prob...	[more]
KAE8646868.1	1.1e-120	76.56	hypothetical protein Csa_020851 [Cucumis sativus]	[more]

BLAST of Spg016400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9J9	2.2e-44	38.55	Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g...	[more]

BLAST of Spg016400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KIR8	6.5e-124	77.94	probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1I774	1.0e-121	76.19	probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A5A7VGP3	6.3e-119	75.00	Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1DUH7	1.8e-118	76.84	probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1GJF6	3.1e-118	75.00	probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Spg016400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53010.1	9.1e-46	41.38	Domain of unknown function (DUF303)	[more]
AT4G34215.1	1.6e-45	38.55	Domain of unknown function (DUF303)	[more]
AT4G34215.2	1.6e-45	38.55	Domain of unknown function (DUF303)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005181	Sialate O-acetylesterase domain	PFAM	PF03629	SASA	coord: 73..330 e-value: 4.1E-64 score: 216.3
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 71..334 e-value: 3.6E-55 score: 189.4
None	No IPR available	PANTHER	PTHR31988	ESTERASE, PUTATIVE (DUF303)-RELATED	coord: 69..333
None	No IPR available	PANTHER	PTHR31988:SF19	BNACNNG62850D PROTEIN	coord: 69..333
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 74..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016400.1	Spg016400.1	mRNA