Spg016332 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016332
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Transcription factor IIIB 90 kDa subunit
Location	scaffold9: 40752002 .. 40756146 (+)
RNA-Seq Expression	Spg016332
Synteny	Spg016332

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACCTCGGACCAAACGGAGACGGAAGACCTCGGCCTCGGCCTCGCCCAAGGCCGAGGCCGAGCAGGGGTCGGGCCAAGGCCCGGTCCCTCCAACCTTGGCCCGTTTCGCCTCCGGAGTCCATTTTTCGATCCTATTTCTGGGCTCATGTCAGGTTGTCCTCGTCAACTCCTTGTATATCGGATTGGTCCAAAATTACCTATAACATAAAATATAACAAAAGTTCGATGTTATTAAAATAGTAATATGAAAAATTTTGTTTCCTATTTTTTTTTTTAAAAAAAAAAGAAAAAAGAATGCAGGTCAAAGCACCACAATATAATTTGTTTCAAAAAAAAAAAAAGCACCACAATATAATTATTTAAAATAAAATAGTAAGTTGATAATCCCTCAATTGATCACTATTACGATTTGCTAAATATTGAACTCCTTGGTTATAATTTATAAGAACTAAACGGGGTGGGGAAAAGGAATGATGAATACATTCCCCCATTCTCGTCTATGTTTAGCTTGTGAAATTTGTTTATTTTCTCCATTACCTTGCTCATTCTCTGTCTGAACGAGGATTTTCCATGGAGGAGAAGAATATTCCTTGCACATTCACTGTAGCTACTTATATTTTTATTTTTCTATAAAAGAGAAACTAAATATAATTAAAAAAAAATTTCCCGTACTAATACAAGCTGTATAATTATTTTTTAAACTTATATAAAAAAATAAAATTATTTTTTAAACTATGTTTGAAATTGTAGAATTGGCCGACCTCGAAGAAAACAATGATGGGCCGGTTCATGTACTAGGCCCTCTATTATTGGGCCATCTCTGAGATGAAGGTCGAGTCCTTTCTTCTCTCATCTGCTTACACCGTATATAAGTTACAATGCGTTTCTTTTTAGGCGGCAACGGATCGTCGTCGAGATTCCGAATTTATTTTCAACTTCTTTTGTTCGCGTTCAATCACTAACCGGCGGTTCCGTTTTCTTCTCTGAGCCTTCTTCATCGACGGCGAGTGATTTGTTTGTGCTCTGCGCATTTCGATCCGTTGATCCATATCGTAAGTATTCCATCAAGTTATTATGCTTACGATCTTTAGCGTTATGTAAGAAAATCTAGGATAGATTGTTCTGGGATTTTTCTGTTTGATTACTCTTTCCCCCTCATGATTGGAACATGTTTAGGTCAAGCATCACCGTTTTTAGCTTTCAGTTATCTAGGTTAGCTTTAAGGTTAACCAATCATGAAATGAAGTTCTACTAAGTTGGCTTTCTGGTGAAGGCGATTCTTCTGCGTTTTTGTAACCGATTTCTGATACACGTCTCAACAACAGAGTGGAAATATAGTAGATGTAGATGTAGTTCTGATGGTCATGTTCTGTATTTTGTATTTGTACACTGAAATAACAACTTTATTTTTGAGAAGGAGATTTTGGAAACTGATAGTCAGTGTAATTCCATTCATATTCTCTAAAACTGCTGCTCAAAAGATGTCGAACGGGCATTAGCATTTCAATTGAATTCGGAAGCCTGTGTTCATTGTTATAATATCTTTGACCATTCCAGTCTGTCTTTCTTATTTGTTATTGAGATGATATGTTTCTGGTCGGACTTATTTTTTCGTTTGAGAAGGCCAACATGCAGAAGGTAGTGTGTAATTGAATGTTGTAATTTCAGAAGGCTTTCTGTTTTTCTTTTATTTATTTATTTTATTTTCCTTTTGAACAACAAAGACTTTCTGGTCTTAGTAGATACTAGATTGTCACTGTCCATAGTTTGGAAAGATATAACATCCAGTTATTGATGCTGATTGCATTTTGATATTGGTAAACGCTGCTGTTGTTTTCAGATGAAAACTGCCAATGCATCATTGTTCAGCATGTTCTGCAAACGTAAAGATAAAAGTTCTCCATCTGATGATGAGTCAGAGGACCTTGGTGATGTTCTTGACAGTGAGGTATCCCTACGACTTAACTGCATTAGCTTTTGGTTTCTTATATTTCTGCTAGTGGTGTTCTAACCGTAGTTTTTCACAAATTATAGGTGAACTTTTACCTAAACAATAAGGAGGAGGCACATTATAAAAAGATTATTTGGGAGAAAATTAACAAAGACTACCTTCAGGTTAAAGGCTTCTTGCCCCTCCACAACCCTTTGATTGCTTCAAGGAAAGTTTTAACTTATAGTTACACTGGGAAAAGGTGATTTACATTTCAATCATAAGAAATGCCATGCATGATTTTCAGGAACAAGCAGCTAAGGAACAAGGTCATCATGTTGCTGGAGCTGCAGCTGTGGTTAAAAAGTCAAAGAAGGTATCTAATCTAACAAACTGCATCTCACCTTAGATAAGAAGTTTCTTATCTTATCCAGAAAAAGATTGAATAGGAGGTTTGCACCTTTACATTTTATGGTATGATTTATGTTTTCTCATACTATTGGAAAGCAGAGGCAAAGGAAAAAGGAAGCTCCAACAAATAACCCAGCTCAAGGCGATACAGAAACTACACGTGAAATGCTAACCAAGAAGGTATATCAGCGGAATAAACTGATGATAAATTCATTTGGTGCACATGTGATTTATCCTATTGAATCTGTTCTATTCTCATTTACAGTTGTTAAGTTTCTTAACTTTGCATTCCTCAGGCTCGTTACTTTCCCGTTCACTAAAACCAACTTCCACTCATAACTATTGCCTAGTTGGTCTTGTATGCACCATATGAATACAATGTGTTTTATGAAATGTTTTGTTGATATTTCTTTTTCTCAAGTAATTTTTCTTAAGATACACTTGAGTGTAGTAATCCGCATGATTATTTTGGCGCAGGGAAGGGAATTTGTTTCTGTTATTCCATGGTTTTTTGCCTGCTAACTTATATTGTTTGGTCGCGTTACATCAATTCACCCAATGACTTGTTTAGCACAATCGTTCTCTGCAGAGGCTCAGTTCGAAAGTCAACTTTGATGTGTTGGAAGAACTCTTCTCTGATGCGGTATATTTATTTATCTGTTTCTACTTTCTTGTTTTGGTTTTGTTAAGTTCATCCTAGTAATTGCATGTTCCAAGGCGAATGTAATTGGACTGTCAGTAGAGTACAGTCAAATAACCTTGTCTGAACAAAGGGTGGAAAAAAGGAGGGAACACAATCTAGCCTTCCTGATGATTTCAGTTAGTATGCAAACACATGATTACTTATAACTACCTAATAATTTGCGTGCAGCCTGCATCTGGTTCAAACAAAAAGCAGGAAGCCTCGAGTTGTGAAGAGAATAGTTCAGCTGGACAAAGCCACTGTAGGAACCAGCTTGAAGAAGACCTCGAACACTACGACTCCAATGTGGTGTTTGAAGAGGATGTTGACACGGAACATGAAAATCCTGCAGAAACTTATGGTAACGAGTATTATGATTTCGAAAACGAGGAAGAGTATGGTTATAGATATGGCGACTATGGTGCAGATGAGGAATATTAGCTGTCATCTGTTTATACCAAAGCAAAGCTTCTTAATTGCCTGAGGTCATGAGATGGATTTCAGCTAGCACAGCGACAACAGCAGCAAGTGCATGAAAGAGCTTTGCATTAATTATAGGTGTTTGACGACAGGTTCAAGGAACAGCAACAGTCATTAACTTCTTTTTACTTTCTCCTCTTTGGAAGTAGCATTGGTCAAAGTTAACGATGGGAAAAAGGAAAGTAAAACTATAGAAGTGTGAAGAGAGGCTTCGATAACACAGAGAGAACGAATGAAGGCGATAGCAAAAAAGGGTAGTAAGAAAGAAGAAACGTGAATGGGGTTAGGTCCATGATTTGTTACGCTTCTTTCAACAAGATTCAAATTTCTGACTTATTGATTATTTTTTTTTTTGACACAAAGAGATAAGATATCATTAATCAATAGTGAGTCCCTTGTGGGTACAAACAATGACTTCTCACTAAGAGTCATATTTTGAATCCAAATAGGAAAATCATCCAACCATAACATCGGAGGAGAGAAAAGACCTTGTCGAGCTAATTTATGAGCAATCCTATTATGATTCCTAGGGGTATACAAAAAATCCACCTTATGGAACTTTTTATGAGCTAATTTCTGACTTCTTGATTGATGACATATGATTTAGTTAATTCATCTATGTTGAGGTTCGCTTTTTT

mRNA sequence

ATGACACCTCGGACCAAACGGAGACGGAAGACCTCGGCCTCGGCCTCGCCCAAGGCCGAGGCCGAGCAGGGGTCGGGCCAAGGCCCGGTCCCTCCAACCTTGGCCCGTTTCGCCTCCGGAGTCCATTTTTCGATCCTATTTCTGGGCTCATGTCAGGTTGTCCTCGTCAACTCCTTGCGGCAACGGATCGTCGTCGAGATTCCGAATTTATTTTCAACTTCTTTTGTTCGCGTTCAATCACTAACCGGCGGTTCCGTTTTCTTCTCTGAGCCTTCTTCATCGACGGCGAGTGATTTGTTTGTGCTCTGCGCATTTCGATCCGTTGATCCATATCATGATATGTTTCTGGTCGGACTTATTTTTTCGTTTGAGAAGGCCAACATGCAGAAGATGAAAACTGCCAATGCATCATTGTTCAGCATGTTCTGCAAACGTAAAGATAAAAGTTCTCCATCTGATGATGAGTCAGAGGACCTTGGTGATGTTCTTGACAGTGAGGTGAACTTTTACCTAAACAATAAGGAGGAGGCACATTATAAAAAGATTATTTGGGAGAAAATTAACAAAGACTACCTTCAGGAACAAGCAGCTAAGGAACAAGGTCATCATGTTGCTGGAGCTGCAGCTGTGGTTAAAAAGTCAAAGAAGCAGAGGCAAAGGAAAAAGGAAGCTCCAACAAATAACCCAGCTCAAGGCGATACAGAAACTACACGTGAAATGCTAACCAAGAAGAGGCTCAGTTCGAAAGTCAACTTTGATGTGTTGGAAGAACTCTTCTCTGATGCGCCTGCATCTGGTTCAAACAAAAAGCAGGAAGCCTCGAGTTGTGAAGAGAATAGTTCAGCTGGACAAAGCCACTGTAGGAACCAGCTTGAAGAAGACCTCGAACACTACGACTCCAATGTGGTGTTTGAAGAGGATGTTGACACGGAACATGAAAATCCTGCAGAAACTTATGGTAACGAGTATTATGATTTCGAAAACGAGGAAGAGTATGGTTATAGATATGGCGACTATGGTGCAGATGAGGAATATTAG

Coding sequence (CDS)

ATGACACCTCGGACCAAACGGAGACGGAAGACCTCGGCCTCGGCCTCGCCCAAGGCCGAGGCCGAGCAGGGGTCGGGCCAAGGCCCGGTCCCTCCAACCTTGGCCCGTTTCGCCTCCGGAGTCCATTTTTCGATCCTATTTCTGGGCTCATGTCAGGTTGTCCTCGTCAACTCCTTGCGGCAACGGATCGTCGTCGAGATTCCGAATTTATTTTCAACTTCTTTTGTTCGCGTTCAATCACTAACCGGCGGTTCCGTTTTCTTCTCTGAGCCTTCTTCATCGACGGCGAGTGATTTGTTTGTGCTCTGCGCATTTCGATCCGTTGATCCATATCATGATATGTTTCTGGTCGGACTTATTTTTTCGTTTGAGAAGGCCAACATGCAGAAGATGAAAACTGCCAATGCATCATTGTTCAGCATGTTCTGCAAACGTAAAGATAAAAGTTCTCCATCTGATGATGAGTCAGAGGACCTTGGTGATGTTCTTGACAGTGAGGTGAACTTTTACCTAAACAATAAGGAGGAGGCACATTATAAAAAGATTATTTGGGAGAAAATTAACAAAGACTACCTTCAGGAACAAGCAGCTAAGGAACAAGGTCATCATGTTGCTGGAGCTGCAGCTGTGGTTAAAAAGTCAAAGAAGCAGAGGCAAAGGAAAAAGGAAGCTCCAACAAATAACCCAGCTCAAGGCGATACAGAAACTACACGTGAAATGCTAACCAAGAAGAGGCTCAGTTCGAAAGTCAACTTTGATGTGTTGGAAGAACTCTTCTCTGATGCGCCTGCATCTGGTTCAAACAAAAAGCAGGAAGCCTCGAGTTGTGAAGAGAATAGTTCAGCTGGACAAAGCCACTGTAGGAACCAGCTTGAAGAAGACCTCGAACACTACGACTCCAATGTGGTGTTTGAAGAGGATGTTGACACGGAACATGAAAATCCTGCAGAAACTTATGGTAACGAGTATTATGATTTCGAAAACGAGGAAGAGTATGGTTATAGATATGGCGACTATGGTGCAGATGAGGAATATTAG

Protein sequence

MTPRTKRRRKTSASASPKAEAEQGSGQGPVPPTLARFASGVHFSILFLGSCQVVLVNSLRQRIVVEIPNLFSTSFVRVQSLTGGSVFFSEPSSSTASDLFVLCAFRSVDPYHDMFLVGLIFSFEKANMQKMKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTETTREMLTKKRLSSKVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDLEHYDSNVVFEEDVDTEHENPAETYGNEYYDFENEEEYGYRYGDYGADEEY

Homology

[Close]

BLAST of Spg016332 vs. NCBI nr
Match: XP_022936233.1 (uncharacterized protein LOC111442904 [Cucurbita moschata])

HSP 1 Score: 305.4 bits (781), Expect = 6.0e-79
Identity = 186/292 (63.70%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 59  LRQRIVVEIPNLFSTSFVRVQSLTGGS---VFFSEPSSSTASDLFVLCAFRSVDPYHDMF 118
           +RQRIVVEIPN  STSF RV  LTGGS   +FFS P SS  SDL +   F S+       
Sbjct: 5   MRQRIVVEIPNFCSTSF-RVLLLTGGSSAYLFFSVPFSSAESDLLLSARFGSL------- 64

Query: 119 LVGLIFSFEKANMQKMKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKE 178
                       +  MKTA ASLFS+ CK KD+ S SDDESEDLGDVLDSEV  YLN KE
Sbjct: 65  ------------ISIMKTAEASLFSLLCKHKDEDSSSDDESEDLGDVLDSEVKTYLNTKE 124

Query: 179 EAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTE 238
           E HYKKIIWE++NKDYLQEQAAK++GH V GAAA+VKKSKK RQR  EAP N PAQ +T+
Sbjct: 125 ETHYKKIIWEQMNKDYLQEQAAKKEGHDVVGAAAIVKKSKK-RQRITEAPINKPAQAETD 184

Query: 239 TTREMLTKKRLSSKVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDL 298
           TTRE+LTKKRLSSK NFDVL++LF+D PA  S+KKQEASS EENS + Q+H  NQLEEDL
Sbjct: 185 TTREILTKKRLSSKFNFDVLDKLFTDTPAPDSSKKQEASSFEENSLSTQNHSTNQLEEDL 244

Query: 299 E---HYDSNVVFEEDVDTEHENPAETYGNEYYDFENEEEYGYRYGDYGADEE 345
           E   H D+ +  E  +D E E+P E YGNEYYD+ENEEEYGY YGDYG +EE
Sbjct: 245 EGNNHVDT-ISEENAIDFEDEDPPEAYGNEYYDYENEEEYGYGYGDYGGEEE 274

[Close]

BLAST of Spg016332 vs. NCBI nr
Match: XP_022975738.1 (uncharacterized protein LOC111475976 [Cucurbita maxima])

HSP 1 Score: 303.9 bits (777), Expect = 1.8e-78
Identity = 185/292 (63.36%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 59  LRQRIVVEIPNLFSTSFVRVQSLTGGSV---FFSEPSSSTASDLFVLCAFRSVDPYHDMF 118
           +RQRIVVEIPN  STS+ RVQ L GGS+   FFSEP SS ASD  +   F S+       
Sbjct: 5   MRQRIVVEIPNFCSTSY-RVQLLIGGSIANLFFSEPLSSAASDFLLSARFGSL------- 64

Query: 119 LVGLIFSFEKANMQKMKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKE 178
                       +  MKTA ASLFS+ CK KD+ S SDD SEDLGDVLDSEVN YLN KE
Sbjct: 65  ------------IYIMKTAEASLFSLLCKHKDEDSSSDDVSEDLGDVLDSEVNSYLNTKE 124

Query: 179 EAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTE 238
           EA YKKIIWE+INKDYLQEQAAK++GH V GAAA+VKKSKK RQR  EAP N PAQ +T+
Sbjct: 125 EARYKKIIWEQINKDYLQEQAAKKEGHDVVGAAAIVKKSKK-RQRITEAPINKPAQAETD 184

Query: 239 TTREMLTKKRLSSKVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDL 298
           TT E+LTKKRLSSK NFDVL++LF+D PA  S+KKQEASS EENS + Q+H  NQLEEDL
Sbjct: 185 TTCELLTKKRLSSKFNFDVLDKLFTDTPAPDSSKKQEASSFEENSLSAQNHSTNQLEEDL 244

Query: 299 E---HYDSNVVFEEDVDTEHENPAETYGNEYYDFENEEEYGYRYGDYGADEE 345
           E   H D+ +  E  +D E E+P E YGNEYYD+ENEEEYGY YG+YG +EE
Sbjct: 245 EGNNHVDT-ISEENAIDFEDEDPTEAYGNEYYDYENEEEYGYGYGNYGGEEE 274

[Close]

BLAST of Spg016332 vs. NCBI nr
Match: XP_022142780.1 (transcription factor IIIB 90 kDa subunit-like isoform X1 [Momordica charantia])

HSP 1 Score: 302.4 bits (773), Expect = 5.1e-78
Identity = 168/215 (78.14%), Postives = 182/215 (84.65%), Query Frame = 0


Query: 131 MKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKD 190
           M+T +ASLFSMF K KDKSSPSDDESED+GD+L+SEVN YLNN++EA YKKIIWEKINKD
Sbjct: 1   MRTKDASLFSMFFKXKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 60

Query: 191 YLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTETTREMLTKKRLSSKV 250
           YLQEQAAK+QG  VAGAA VVKKSKK RQRKKEA TN PA+ DTE TREMLTKKRLSSKV
Sbjct: 61  YLQEQAAKKQGRDVAGAAHVVKKSKK-RQRKKEAATNTPAEADTEATREMLTKKRLSSKV 120

Query: 251 NFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDLEHYDSNVVFEEDVDT 310
           NFDVL+ELFSD P S SNKKQ AS+CEENSS  QSHCR     DLE  D + V EEDVDT
Sbjct: 121 NFDVLDELFSDMPVSCSNKKQGASNCEENSSFDQSHCR-----DLEDNDPHAVLEEDVDT 180

Query: 311 EHENPAETYGNEYYDFENEEEYGYRYGDYGADEEY 346
           EHE+P ETYGNEYYD ENEEEYG+ YGDYG DEEY
Sbjct: 181 EHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 209

[Close]

BLAST of Spg016332 vs. NCBI nr
Match: KAG6591886.1 (hypothetical protein SDJN03_14232, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.9 bits (751), Expect = 1.8e-75
Identity = 180/294 (61.22%), Postives = 209/294 (71.09%), Query Frame = 0


Query: 57  NSLRQRIVVEIPNLFSTSFVRVQSLTGGSV---FFSEPSSSTASDLFVLCAFRSVDPYHD 116
           +S R RI + +P        RVQ LTGG+    FFSEP SS ASDL +   F S+     
Sbjct: 10  SSSRFRIFIRLP-------FRVQLLTGGTAAYFFFSEPLSSAASDLLLSARFGSL----- 69

Query: 117 MFLVGLIFSFEKANMQKMKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNN 176
                         +  MKTA ASLFS+ CK KD+ S SDDESEDLGDVLDSEV  YLN 
Sbjct: 70  --------------ISIMKTAEASLFSLLCKHKDEDSSSDDESEDLGDVLDSEVKTYLNT 129

Query: 177 KEEAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGD 236
           KEEAHYKKIIWE++NKDYLQEQAAK++GH V GAAA+VKKSKK RQR  EAP N PAQ +
Sbjct: 130 KEEAHYKKIIWEQMNKDYLQEQAAKKEGHDVVGAAAIVKKSKK-RQRITEAPINKPAQAE 189

Query: 237 TETTREMLTKKRLSSKVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEE 296
           T+TTRE+LTKKRLSSK NFDVL++LF+D PA  S+KKQEASS EENS + Q+H  NQLEE
Sbjct: 190 TDTTREILTKKRLSSKFNFDVLDKLFTDTPAPDSSKKQEASSFEENSLSTQNHSTNQLEE 249

Query: 297 DLE---HYDSNVVFEEDVDTEHENPAETYGNEYYDFENEEEYGYRYGDYGADEE 345
           DLE   H D+ +  E  +D E E+P E YGNEYYD+ENEEEYGY YGDYG +EE
Sbjct: 250 DLEGNNHVDT-ISEENAIDFEDEDPPEAYGNEYYDYENEEEYGYGYGDYGGEEE 275

[Close]

BLAST of Spg016332 vs. NCBI nr
Match: XP_023536162.1 (uncharacterized protein LOC111797407 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-74
Identity = 179/279 (64.16%), Postives = 204/279 (73.12%), Query Frame = 0


Query: 72  STSFVRVQSLTGGS---VFFSEPSSSTASDLFVLCAFRSVDPYHDMFLVGLIFSFEKANM 131
           STSF RVQ LTGGS   +FFSEP S  ASDL     F            G + +      
Sbjct: 19  STSF-RVQLLTGGSSAYLFFSEPLSLAASDLLRSARF------------GCLINI----- 78

Query: 132 QKMKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKIN 191
             MKTA ASLFS+ C+ KD+ S SDDESEDLGDVLDSEVN YLN KEEAHYKKIIWE++N
Sbjct: 79  --MKTAEASLFSVLCEHKDEDSSSDDESEDLGDVLDSEVNSYLNTKEEAHYKKIIWEQMN 138

Query: 192 KDYLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTETTREMLTKKRLSS 251
           KDYLQEQAAK++GH V GAAA+VKKSKK RQR  EAP N PAQ +T+ TREMLTKKRLSS
Sbjct: 139 KDYLQEQAAKKEGHDVVGAAAIVKKSKK-RQRVTEAPINKPAQAETDATREMLTKKRLSS 198

Query: 252 KVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDLE---HYDSNVVFE 311
           K NFDVL++LF+D PA  S+KKQEASS EENS + Q+H  NQLEEDLE   H D+ +  E
Sbjct: 199 KFNFDVLDKLFTDTPAPDSSKKQEASSFEENSLSAQNHSMNQLEEDLEGNNHVDT-ISEE 258

Query: 312 EDVDTEHENPAETYGNEYYDFENEEEYGYRYGDYGADEE 345
             +D E E+P E YGNEYYD+ENEEEYGY YGDYG +EE
Sbjct: 259 NAIDFEDEDPTEAYGNEYYDYENEEEYGYGYGDYGGEEE 275

[Close]

BLAST of Spg016332 vs. ExPASy Swiss-Prot
Match: Q92994 (Transcription factor IIIB 90 kDa subunit OS=Homo sapiens OX=9606 GN=BRF1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.6e-05
Identity = 48/149 (32.21%), Postives = 73/149 (48.99%), Query Frame = 0


Query: 143 CKRKDKSSPSD---DESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKDYLQEQAAKE 202
           C     S P D   D   DL  + D E++ Y+ N+ EA  K  +W + N +YL+EQ  KE
Sbjct: 430 CISSQSSDPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKE 489

Query: 203 QGHHVAGAAAVVKKSK----KQRQRKKEAPTNNPAQGDT--ETTREMLTKKRLSSKVNFD 262
                   A + K+ +    K+ + KK      P Q  T  E   +ML +K++SSK+N+ 
Sbjct: 490 --------ARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAIEKMLEQKKISSKINYS 549

Query: 263 VLEELFSDAPASGSNKKQEASSCEENSSA 283
           VL  L S   A G +  +E +  E ++SA
Sbjct: 550 VLRGLSS---AGGGSPHREDAQPEHSASA 567

[Close]

BLAST of Spg016332 vs. ExPASy Swiss-Prot
Match: Q9P6R0 (Transcription factor IIIB 60 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=brf1 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 2.1e-05
Identity = 33/109 (30.28%), Postives = 62/109 (56.88%), Query Frame = 0


Query: 154 DESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKK 213
           +E   L DV D E+   L +K+E   K  +W ++NKDYL E+ AK           +V++
Sbjct: 381 EEEVTLEDVDDDEIEDILLDKDEILTKTQVWMELNKDYLAEEEAKNLKLQEDLKKGIVRQ 440

Query: 214 SKKQRQ-RKKEAPTNNPAQGDTETTREMLTKKRLSSKVNFDVLEELFSD 262
            +K+R+ R +++ ++  A    E+ +EM+ ++  S K+N++ L+ LF +
Sbjct: 441 PRKRRRYRPRDSTSDGIADTAAESAKEMMQQRAFSKKINYEALDMLFDE 489

[Close]

BLAST of Spg016332 vs. ExPASy Swiss-Prot
Match: Q8CFK2 (Transcription factor IIIB 90 kDa subunit OS=Mus musculus OX=10090 GN=Brf1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.0e-04
Identity = 43/149 (28.86%), Postives = 73/149 (48.99%), Query Frame = 0


Query: 147 DKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKDYLQEQAAKEQGHHVAG 206
           D    S D   DL  + D E++ Y+ N+ EA  K  +W + N +YL+EQ  KE       
Sbjct: 436 DPKDSSGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQKEKEARIAKEK 495

Query: 207 AAAVVKKSKKQRQRKKEAPTNNPAQGDTETTREMLTKKRLSSKVNFDVLEELFS---DAP 266
              + K+ K ++  K+  P      G  E   +ML +K++SSK+N+ VL +L S    +P
Sbjct: 496 ELGIYKEHKPKKSCKRREPILASTAG--EAIEKMLEQKKISSKINYSVLRDLNSKGGGSP 555

Query: 267 ASGSNKKQEASSCEENSSAGQSHCRNQLE 293
               ++  E +S ++ S   ++  RN  +
Sbjct: 556 PRDDSQPPERASTKKLSRRKRATTRNSAD 582

[Close]

BLAST of Spg016332 vs. ExPASy TrEMBL
Match: A0A6J1FCP7 (uncharacterized protein LOC111442904 OS=Cucurbita moschata OX=3662 GN=LOC111442904 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.9e-79
Identity = 186/292 (63.70%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 59  LRQRIVVEIPNLFSTSFVRVQSLTGGS---VFFSEPSSSTASDLFVLCAFRSVDPYHDMF 118
           +RQRIVVEIPN  STSF RV  LTGGS   +FFS P SS  SDL +   F S+       
Sbjct: 5   MRQRIVVEIPNFCSTSF-RVLLLTGGSSAYLFFSVPFSSAESDLLLSARFGSL------- 64

Query: 119 LVGLIFSFEKANMQKMKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKE 178
                       +  MKTA ASLFS+ CK KD+ S SDDESEDLGDVLDSEV  YLN KE
Sbjct: 65  ------------ISIMKTAEASLFSLLCKHKDEDSSSDDESEDLGDVLDSEVKTYLNTKE 124

Query: 179 EAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTE 238
           E HYKKIIWE++NKDYLQEQAAK++GH V GAAA+VKKSKK RQR  EAP N PAQ +T+
Sbjct: 125 ETHYKKIIWEQMNKDYLQEQAAKKEGHDVVGAAAIVKKSKK-RQRITEAPINKPAQAETD 184

Query: 239 TTREMLTKKRLSSKVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDL 298
           TTRE+LTKKRLSSK NFDVL++LF+D PA  S+KKQEASS EENS + Q+H  NQLEEDL
Sbjct: 185 TTREILTKKRLSSKFNFDVLDKLFTDTPAPDSSKKQEASSFEENSLSTQNHSTNQLEEDL 244

Query: 299 E---HYDSNVVFEEDVDTEHENPAETYGNEYYDFENEEEYGYRYGDYGADEE 345
           E   H D+ +  E  +D E E+P E YGNEYYD+ENEEEYGY YGDYG +EE
Sbjct: 245 EGNNHVDT-ISEENAIDFEDEDPPEAYGNEYYDYENEEEYGYGYGDYGGEEE 274

[Close]

BLAST of Spg016332 vs. ExPASy TrEMBL
Match: A0A6J1IK57 (uncharacterized protein LOC111475976 OS=Cucurbita maxima OX=3661 GN=LOC111475976 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 8.5e-79
Identity = 185/292 (63.36%), Postives = 213/292 (72.95%), Query Frame = 0


Query: 59  LRQRIVVEIPNLFSTSFVRVQSLTGGSV---FFSEPSSSTASDLFVLCAFRSVDPYHDMF 118
           +RQRIVVEIPN  STS+ RVQ L GGS+   FFSEP SS ASD  +   F S+       
Sbjct: 5   MRQRIVVEIPNFCSTSY-RVQLLIGGSIANLFFSEPLSSAASDFLLSARFGSL------- 64

Query: 119 LVGLIFSFEKANMQKMKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKE 178
                       +  MKTA ASLFS+ CK KD+ S SDD SEDLGDVLDSEVN YLN KE
Sbjct: 65  ------------IYIMKTAEASLFSLLCKHKDEDSSSDDVSEDLGDVLDSEVNSYLNTKE 124

Query: 179 EAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTE 238
           EA YKKIIWE+INKDYLQEQAAK++GH V GAAA+VKKSKK RQR  EAP N PAQ +T+
Sbjct: 125 EARYKKIIWEQINKDYLQEQAAKKEGHDVVGAAAIVKKSKK-RQRITEAPINKPAQAETD 184

Query: 239 TTREMLTKKRLSSKVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDL 298
           TT E+LTKKRLSSK NFDVL++LF+D PA  S+KKQEASS EENS + Q+H  NQLEEDL
Sbjct: 185 TTCELLTKKRLSSKFNFDVLDKLFTDTPAPDSSKKQEASSFEENSLSAQNHSTNQLEEDL 244

Query: 299 E---HYDSNVVFEEDVDTEHENPAETYGNEYYDFENEEEYGYRYGDYGADEE 345
           E   H D+ +  E  +D E E+P E YGNEYYD+ENEEEYGY YG+YG +EE
Sbjct: 245 EGNNHVDT-ISEENAIDFEDEDPTEAYGNEYYDYENEEEYGYGYGNYGGEEE 274

[Close]

BLAST of Spg016332 vs. ExPASy TrEMBL
Match: A0A6J1CLW7 (transcription factor IIIB 90 kDa subunit-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012793 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 2.5e-78
Identity = 168/215 (78.14%), Postives = 182/215 (84.65%), Query Frame = 0


Query: 131 MKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKD 190
           M+T +ASLFSMF K KDKSSPSDDESED+GD+L+SEVN YLNN++EA YKKIIWEKINKD
Sbjct: 1   MRTKDASLFSMFFKXKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 60

Query: 191 YLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDTETTREMLTKKRLSSKV 250
           YLQEQAAK+QG  VAGAA VVKKSKK RQRKKEA TN PA+ DTE TREMLTKKRLSSKV
Sbjct: 61  YLQEQAAKKQGRDVAGAAHVVKKSKK-RQRKKEAATNTPAEADTEATREMLTKKRLSSKV 120

Query: 251 NFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDLEHYDSNVVFEEDVDT 310
           NFDVL+ELFSD P S SNKKQ AS+CEENSS  QSHCR     DLE  D + V EEDVDT
Sbjct: 121 NFDVLDELFSDMPVSCSNKKQGASNCEENSSFDQSHCR-----DLEDNDPHAVLEEDVDT 180

Query: 311 EHENPAETYGNEYYDFENEEEYGYRYGDYGADEEY 346
           EHE+P ETYGNEYYD ENEEEYG+ YGDYG DEEY
Sbjct: 181 EHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 209

[Close]

BLAST of Spg016332 vs. ExPASy TrEMBL
Match: A0A1S4DV37 (uncharacterized protein LOC103487591 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487591 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 2.4e-73
Identity = 155/216 (71.76%), Postives = 182/216 (84.26%), Query Frame = 0


Query: 131 MKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKD 190
           MK A+ASLFSMF + KDKSSPS+D+SEDLGDV DSEVN YLNN++EAHYKKIIWE+INKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 191 YLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDT-ETTREMLTKKRLSSK 250
           YLQ+QAAK+QG ++ GAAAVVKKSKKQR+RK EAP N PAQ DT ETT EML KKRLSSK
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKQRKRKIEAPINKPAQADTEETTHEMLIKKRLSSK 120

Query: 251 VNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDLEHYDSNVVFEEDVD 310
            +FDVL++L  D PA  S++KQ ASSC+ENSS+GQSHC N+LE+D E   +  VFEE++D
Sbjct: 121 FDFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLD 180

Query: 311 TEHENPAETYGNEYYDFENEEEYGYRYGDYGADEEY 346
            E E+P ETYGNEYYDF+NEEEYGY Y DYG +EEY
Sbjct: 181 IEGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 216

[Close]

BLAST of Spg016332 vs. ExPASy TrEMBL
Match: A0A5D3E7S6 (Transcription factor IIIB 90 kDa subunit-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold398G00100 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 1.7e-71
Identity = 154/216 (71.30%), Postives = 181/216 (83.80%), Query Frame = 0


Query: 131 MKTANASLFSMFCKRKDKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKD 190
           MK A+ASLFSMF + KDKSSPS+D+SEDLGDV DSEVN YLNN++EAHYKKIIWE+INKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 191 YLQEQAAKEQGHHVAGAAAVVKKSKKQRQRKKEAPTNNPAQGDT-ETTREMLTKKRLSSK 250
           YLQ+QAAK+QG ++ GAAAVVKKSKK R+RK EAP N PAQ DT ETT EML KKRLSSK
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKK-RKRKIEAPINKPAQADTEETTHEMLIKKRLSSK 120

Query: 251 VNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDLEHYDSNVVFEEDVD 310
            +FDVL++L  D PA  S++KQ ASSC+ENSS+GQSHC N+LE+D E   +  VFEE++D
Sbjct: 121 FDFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLD 180

Query: 311 TEHENPAETYGNEYYDFENEEEYGYRYGDYGADEEY 346
            E E+P ETYGNEYYDF+NEEEYGY Y DYG +EEY
Sbjct: 181 IEGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 215

[Close]

BLAST of Spg016332 vs. TAIR 10
Match: AT3G09360.1 (Cyclin/Brf1-like TBP-binding protein )

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-17
Identity = 78/224 (34.82%), Postives = 110/224 (49.11%), Query Frame = 0


Query: 147 DKSSPSDDESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKDYLQEQAAKEQGHHVA- 206
           ++ + + DES++  D+ D EVN Y+NN+EE HYK I W ++NKDYL+EQAAKE     A 
Sbjct: 387 EEHADTSDESDNFSDISDDEVNGYINNEEETHYKTITWTEMNKDYLEEQAAKEAALKAAS 446

Query: 207 -------------------GAAAVVKKSKKQRQRKKEAPTNNPAQGDT--ETTREMLTKK 266
                               A A   KS+K++Q+KK     N A   T  E  R  L KK
Sbjct: 447 EALKASNSNCPEDARKAFEAAKADAAKSRKEKQQKKAEEAKNAAPPATAVEAVRRTLDKK 506

Query: 267 RLSSKVNFDVLEELFSDAPASGSNKKQEASSCEENSSAGQSHCRNQLEEDLEHYDSNVVF 326
           RLSS +N+DVLE LF  +    S K+ +  +  E         ++   E+ E+ D     
Sbjct: 507 RLSSVINYDVLESLFDTSAPEKSPKRSKTETDIEKKKEENKEMKSNEHENGENED----- 566

Query: 327 EEDVDTEHENPAETY--------GNEYY-DFENEEEYGYRYGDY 340
            ED + E E   E+Y        G ++Y + E EEE G  +G Y
Sbjct: 567 -EDEEDEEEGNVESYDMKTDFQNGEKFYEEDEEEEEDGNDFGLY 604

[Close]

BLAST of Spg016332 vs. TAIR 10
Match: AT4G19550.1 (zinc ion binding;transcription regulators )

HSP 1 Score: 43.9 bits (102), Expect = 3.0e-04
Identity = 54/187 (28.88%), Postives = 88/187 (47.06%), Query Frame = 0


Query: 154 DESEDLGDVLDSEVNFYLNNKEEAHYKKIIWEKINKDYLQEQAAKEQGHHVAGAAAVVKK 213
           D +EDL D+ D+EV   L+N +E   KK  WE +N +Y + +  K            V  
Sbjct: 30  DLAEDLSDISDAEVAENLSNNKEFILKKRAWELMNPEYQKGKYRK------------VTT 89

Query: 214 SKKQRQRKKEAPTNNPAQGDTETTREMLTKKRLSSKVNFDVLEELF--SDAPASGSNKK- 273
            KK+    K AP+   +   TE+  E   KKR S ++N++VL++LF   ++P      K 
Sbjct: 90  RKKKDPANKIAPSKKTSATKTESNAETENKKRPSLEINYNVLDKLFDPENSPKRAKLDKP 149

Query: 274 ----QEASSCEENSSAGQSHCRNQLEEDLEHYDSNVVFEEDVDTE--HENPAETYGNEYY 332
                +    ++NS   +S  ++Q  E+ E       + ED   E  +    E YGNE  
Sbjct: 150 VVVGDQIEYSKQNSE--ESLLKSQYSEEEEEEAEEPDWNEDYSNEDAYRGNEECYGNENL 202

The following BLAST results are available for this feature:

BLAST of Spg016332 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022936233.1	6.0e-79	63.70	uncharacterized protein LOC111442904 [Cucurbita moschata]	[more]
XP_022975738.1	1.8e-78	63.36	uncharacterized protein LOC111475976 [Cucurbita maxima]	[more]
XP_022142780.1	5.1e-78	78.14	transcription factor IIIB 90 kDa subunit-like isoform X1 [Momordica charantia]	[more]
KAG6591886.1	1.8e-75	61.22	hypothetical protein SDJN03_14232, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023536162.1	1.2e-74	64.16	uncharacterized protein LOC111797407 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg016332 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q92994	1.6e-05	32.21	Transcription factor IIIB 90 kDa subunit OS=Homo sapiens OX=9606 GN=BRF1 PE=1 SV...	[more]
Q9P6R0	2.1e-05	30.28	Transcription factor IIIB 60 kDa subunit OS=Schizosaccharomyces pombe (strain 97...	[more]
Q8CFK2	1.0e-04	28.86	Transcription factor IIIB 90 kDa subunit OS=Mus musculus OX=10090 GN=Brf1 PE=1 S...	[more]

BLAST of Spg016332 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FCP7	2.9e-79	63.70	uncharacterized protein LOC111442904 OS=Cucurbita moschata OX=3662 GN=LOC1114429...	[more]
A0A6J1IK57	8.5e-79	63.36	uncharacterized protein LOC111475976 OS=Cucurbita maxima OX=3661 GN=LOC111475976...	[more]
A0A6J1CLW7	2.5e-78	78.14	transcription factor IIIB 90 kDa subunit-like isoform X1 OS=Momordica charantia ...	[more]
A0A1S4DV37	2.4e-73	71.76	uncharacterized protein LOC103487591 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5D3E7S6	1.7e-71	71.30	Transcription factor IIIB 90 kDa subunit-like isoform X3 OS=Cucumis melo var. ma...	[more]

BLAST of Spg016332 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09360.1	1.1e-17	34.82	Cyclin/Brf1-like TBP-binding protein	[more]
AT4G19550.1	3.0e-04	28.88	zinc ion binding;transcription regulators	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.5.650	Single helix bin	coord: 137..201 e-value: 3.8E-17 score: 63.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 264..288
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 289..318
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 328..345
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 203..242
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 262..345
IPR011665	Brf1, TBP-binding domain	PFAM	PF07741	BRF1	coord: 161..260 e-value: 6.0E-25 score: 87.5
IPR000812	Transcription factor TFIIB	PANTHER	PTHR11618	TRANSCRIPTION INITIATION FACTOR IIB-RELATED	coord: 147..261
IPR029529	Transcription factor IIIB subunit Brf1	PANTHER	PTHR11618:SF4	TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT	coord: 147..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016332.1	Spg016332.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
biological_process	GO:0070897	transcription preinitiation complex assembly
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0016020	membrane
cellular_component	GO:0000126	transcription factor TFIIIB complex
molecular_function	GO:0000995	RNA polymerase III general transcription initiation factor activity