Spg016300 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016300
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCytochrome b5 heme-binding domain-containing protein
Locationscaffold9: 39630413 .. 39636640 (-)
RNA-Seq ExpressionSpg016300
SyntenySpg016300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAGTTTAGGAGTATAATTGAAATAAATCACACACTTATTGGAAAATATTTTATAATTTAAAGAGAGAAAAAACAAAACCCCGCAAAGAATAATACACAACCCGACAACCGTACATTGCCATTCGGGTCCAGAACTATGGCCAGTGATCTCTCTCCGCAAAGACCAAGCGAAGACGCGCATGAACAGAAGAGGCCGAGCTCGGTGAAGGGCATAGCTATGGCGGGAAAGATCACGGGGTGGGTTTTGGCTCTTATGGTGGCTATTGTTGCGCTTCTCATTGGGTTTTCCTTCAAATCCCGATACCCGATCTCCTTCTCCTCCCCCTCCCCTGAACAGGTATGAGCTTCTTCTTGTTGAAGCCCGATACAGATCGCATGATTGTTAATTATGCCTGTATTTTAGTTTCGAAAGACATTTAGAATTTCTATCTTCTGTTGGAAGGCATTTCTCTTTTTCTGTCATTAAGAGTATGCTGGCACTTTACGAATTTTGGGAGTATATTTTTTTGGAAAGCTGCAATTGTGACTTGTTAGAGCTGCGTCTATGATTATCAAGCTGTTTAAGATATTTTGTTCGTTCTACACATGGGCAAGTTTTTGGCATGCTAGTTAATGTTATGGAACAAAATTTAAAGATTGAAGGTGAGAAGTTTTCCCATAGTTTGGTTACCGTTTCAAGTTGGGGGAAAGATGGCCTTTGGTTAAATGCTTTGAGATGGTGCTTCTAGCGTTGTTTTCTGCTGCTATAGCTTAATGATTGATTATCGTATTACTCTTTGGACTTTTGGGATCCTGTGATTAGAGCTTGATAAATGGGAGAAATCTACTCTCTCCATGGGGGATGGGAAAGCTCCATGTGGTATAAGATTATTGTGAGTAAATATAGGTCTCATCCTTTTGAGTGGTCGATAGGTGGGGTGAAAGGCACGTCCAAAAACCCTTGGAAAGACATTGTTAGGGAGCTCCCTTCCTTTTCTGACCTTGTGTATAATGTGGTGGGGGATGGGAAAGATACGTATTTCTAGGAGGATAGATGGGTGGGGGATAGACCCCTCTATCTTACCTTTCCTCGTCTCTTCCAGTTGTCATTAACAAAAAAACTGATCGGTGGTAGAAGTTCTTTTTCATTCAGGGAGCTCCCCCTTGTATTCTTTCGAGTTCCGTCGTGTTTTGTCCGATAAGAAAGCGTTGGATGTCACGACTCTTCTTTCTTTGATTGAGGAGTTTGAGTTCAGGTTAGGGAGTAAAGATCGTTGTTTATGGAGTCCTAGCCCCTACGAGGGATTTTCGCGTAAATCATACTTCTGTTCGTTGTTAAATCCTCTTCTCAGTAATGAATCCATTTTCGGGCATCTTCTTTATTTGGAAAGTTCCGAATAAGGTGAAGTTCTTTATTTGGCAAGTTCTCCATAGTAGAGTTAATACTTTTGATAGACTCGTGAGAAGAAATTATCCTTTGTTTGGCCCGTTATGCTATATTCTGTGTCGAAAGGCGGAGGAAGACTTGGATCATATTTTATGGAGATGTGCTTTTGCTCACTCAGTTTGGGAGCTTTTTTTCGCCTCTTTTGGGAGACATCCGATGGTCTTTCAGGACGTGAGAGCGATGATCGAAGAGTTCCTTCTCGATCCGATTTACTGAGGCAAAGGAGGATTCTTGCGGCTAGCCGACATGTTGGTGCTTTTATGGAACTTGTGGGAGGAAAGAAACAATAAGATTTTTAGGGGGTTAGTTAGAAGCCTGGGAGAACTGTGGTCCATTATTAGATACCATGTCTCCTTGTGGGCTTCGATCACGAATCTTTTTTGTAATTGTCCATTAGGTCTCATTATCCTTGATTGGAGAGTCAGAGACCCTTTCTTTGAGAGGGCTCCCTTTTGTGGGCTTGGTTTTTTGTCTGCCCTAGTATTCTTTCATTTTTTCTCAATGAAAGTTGGTTTTTCATTGGGTCCCCTTGGCTAAATAAGTCTTATCAAGCATTCCAACCTATATTCTGCCTAATATCCCTCTCAAAGTGGTTAAATTGATTGAAAGGAAGATTGGAGACTTTATATTTGGAAGATCTGAGACATGTGGTCTTGGCTTTGAAGTGGTTGTGGCATGAAAGGGGCTCTTTTTAACAATCAATTATAGCTAGCGAAGAGAGGGAGGTTTATTGTTAATACAAATGCTCTGGTTGAAGAGGCCCCTAGAATTTGATGAAGGTTTCCTTATCTTATGAGTGAGCACCGGTGAAAAAATTGACTTTTGGAAGGACTTGTGGATCAGCTTTTGTTTGGTTACTTGCTCAAATGCAGATCAAGTAGCAGTTGATGATTTGGTGATCAAATGATTTATTAATCCTAAGGTTTTTTTTTTTTTTTGATAAGAAACAGAAAGGATATATATTCACAAGCAAAAGCAACCATACAGCCTAAGGGCCAAGTTGATCATCATCACAGATAGTAGTGTACCATCTGCAAAGTATAGATTTGATAGGTGAATTTTATCTTCACTAACCTCATTCATTATATAACCTACAATCACAAAGAGGAAGGCAGAAATTGAGTCTCTTTCCTTGATCCCCTAAATGCAACCACCCTATTCCTAAGCTTTCCATTGGGGAATATGGAGAATTTGATGTCTGAGATACTCCGCATCCAACTCATCCATCTAGGCACGGTTGTTTTAACACCCCCAACTCCACCCTCACCAAAGGGTACCTCCAGAAAACCTTAGCCTCTGTCAAACCGAAGCGGGCATCTTCTTCTTCTCCTCTCTCTCTATCTTTTAATGTTTTCCTTTATTTTTTATAATTTAAATCTATCAAACATGTTGATGAAGAGATATAGACCTCAATTAATTGATTTAAGTTGGTAGAGTTCTATACTTTTATTTCAAGTTCTCGTTTTCTTTTGTAGAAACTCTTCAAAGTTGATGAGCTAGCTTTGTATAATGGAACTGATGAGGGATTGCCTATTCTTCTGGGAATTCTTGGGTACTCTCCACCTCCAGACTTTTTTCTATGGTTTTTTTTTGTTTTTTTGTTTTTTTGTTTTGTTTTTGTTTTGTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTTAAAAATTTGTTTTAATTATTGACATTCTTGATGAATCATTTTGACTAATGAGAGTATTTAAAAAATGGAGAAAGTAAATTGTGATAATTCAAAGAACCACTGAGCTGCATATCTCAATTCTTTCTTGTGGTTGCTCTTTTGAAATTATATTATTATTCCGCACATAAGAAATATATATATATATATTTTTTTTATAGGAAACATAAACCATAAGAAATAGTGTTACAATTAGCATGCACATAAGAAATACAAAAGGTCTTGGGATAAGACATCCTTAACCTGACAACATATAGAAAAGCATGCCAATTGTTATACGGTTAAGTAAAGGGGTCATTTTATGGAAATCAGTCCCTTGCTATTTAACCTCTAATAATTTTATTTTTTCAGATCAGTATTTGATGTCACAAAAGGAAAATCTCACTATGGTGTGGGAGGAGGTTATCATCACTTTGCTGGAAGGTTTGTGATCTCTACTTTCCTTGTTAATCTCCTATGCATCTTCTGCTATTTCTTATACGGCATGGCAATGATTCCATATTTGTTAAGAATAAATTTTAAAATTGCTATGTTGAAGCATTTGTTCTATCAATAGTTCGAGCATTAATCTCTTTTCATTATATCAATGAAAAGCTTCGTTTCTTGTTAAAAAATAGTTCACTTGCATTGTTTCTTTTTCTTTGTCTATTTGTGTTTGTCTTGTGTGGCTACTGAATCTAATAGAATATCTTAACAGGTCATTTTCCTTCTGCTCTATTTGCCACCTAGGAAAGTATGGAAGCCTTTTATAAAACATGAAATTTCAACTTAAATGTTACTAGTTCCTTACTCATCCAACTATATTTGATTTTTAACGAGACCTTGATAATTGATAACTGATTGCCACTTTTCTTTGAGTCTTGCAAAGTGCACATTCGGTAATGCACTGATTTGTATTTTTTCATGGTTGTTTCTAAAAACAGAGATGCTTCTCGTGCATTTGTATCAGGAAATTTCACAGGTATTTATGGTTTCTTATTGGGACGGAGAAAAATATTCTGTCAATTCCTTTTTATAACTTTGTTTATGTAATATAACAAACATCTGAGTATCCTAGAGATACAGTGAAATTTTTATTTTCTTTGAGCTGTCAATATGAATCAATTTCTAACCAATGCTTTTCTTTACTATATTGTGAACAGAATACCTAAAATTAAGGTCTTTTTAGGTTGAGGATGTGTATCCTCAATTATCTCTTGTATTAAAATGTCGTCATCATAATTATGCCTTTTTCTCTCTTTTTTTTTTGGGGGGGGGGGGGGGGGTGGTGGTGGTGGTGTAAGGGTGAGGTGAGGAAGGGAGGGAAAGAGTGGTTCGACAGAAGAAAATTTGTTATTACCTAAACATCATTCAAGTAATTGAGTTCTATCTTTTGTTATTGTCATAGGGGATGGACTCACCGATTCTTTGCGGGGTTTGTCAAATACTGAGGTACTCTTATTGCTTGTTTAATTTTTCACGAAGGGCAAGGAATTCCCCTTAAATATGTGGTGCTGTTCCTTATTATTTGTGCTCTCATTCTTTAGCTTTGTAGAACTTGGGCCCCCTCCAAAACCTTCCTTTCCCCTCTTTGGTGTAATGGTGCAATTCTCTCTTGACATTTCAATGAATCATTCTTTATCATTGAATAAAATTGATCATTTTTTCTTATTGCATGGTTGGGCCCAGCATCAAAGACCTCACTGTACATTATACATCTACTTGCTTATATATCTGACAGTTGTACTAACTATCTTGTAGATCAAGAGTGTTGTTGAATGGCGGAGCTTTTACCATAAAACCTACACGTAAGTTTTCTGTGATAGTGTCTATTATCTTAAATGATAGGGGATGACAATTGTTGCAAGTGTCGTTCATAAATTTCGAGTAAAAAAATTTGATCAATTGATCAAAAGGCGATAGATTTGAGAATGACATGCTATTATGGGAATAAATGATCGAGGATCATGCTCTTGGCCTTCACTACAGATTTGTAGGTAAGTTGGTGGGTCTTTACTATGATGATCAAGGAAATCCCACTAAACATTTAAAGGGAGCTGAGGCAAAGGCTGCTAGAGGCGCACAACTGTTGAAGAAACAAAAGAAAGAGGAAGATAAACTGCCAAGTTGTAATTCAAAATGGAGTCAGGCAGAAGGTGGTGAGGTATATACTCTACGAAGTATTCTTGGGATTTTCCTTTCAGCTTCTATGTTTCCTTGTTATAACTGTATCTTGGATTCATGCAATCTACCTAACAGATATATTTGATTATGAATCAAGTATTACGTTCTTTCATTTAAATACACGCACACGCAGAATAAACTTCAGATTGTGACTCTTTTACCCCACCTTTAGGTCTGGTGTGATGATGGCTTCCCGAGGTTGGTACAGAGGCCATTAGAAATTGCATTAACAGGGAAGATGAGGAAACGATGCGCTTGCTTTAGAGAAGACCAACTGAGTGAGCCGGGATTGGAAGTCTATGAAGGTTGTGACTACCTAGCCAAGACTTGCCGTGTTTAAAGAGCCATTACAAACAGCCTTAGCCAGTTCATTATTTAGTCATCATCCAGATATTAGAGGGAGGTGCAACCTGCAGGAGATTGACTGAAATGGCCAATAGATACGGCAATACGACATACCGAATTTTTTGTTCTCTTTTTTTCCTTCCGGTTGGCCGCGGGGGGTGGGGAAGATCGACCGTGTACAGGTATGTGATTATCTACTTCCATGGCACAATACTGTTCATTGATCATCATCAGTTTTCAGCTGCCTGCCTGCTTTCTAGATCTGTGTTTGCTTTTTCATTGTCAATAAGATTTTGATGCATTTTGATGTGAGTTGTGTTTATTGCCCCAGCTGCAAGACTTGAAGAGACAAACCTTGTCTCTCAATTCCGATGTTGCTACACTTCTAACTATTGATATATGCTCCCCTCGTGACATAGCTTTCGAGCTTGATTTGTTTGCCTTTTAACGAAATATGGTTGAATCCTTATGAACTAAAATTCAAAATCGAAAAGGTAGCTTCATTGGTTTAGCATTTGTTCTTAGTTTCCTTTTCATTTTTCGTAAATGAAATACTGATCAATCGAAACAATGTGCAATATTTTAGATACAAA

mRNA sequence

ATGGCCAGTGATCTCTCTCCGCAAAGACCAAGCGAAGACGCGCATGAACAGAAGAGGCCGAGCTCGGTGAAGGGCATAGCTATGGCGGGAAAGATCACGGGGTGGGTTTTGGCTCTTATGGTGGCTATTGTTGCGCTTCTCATTGGGTTTTCCTTCAAATCCCGATACCCGATCTCCTTCTCCTCCCCCTCCCCTGAACAGCTTGATAAATGGGAGAAATCTACTCTCTCCATGGGGGATGGGAAAGCTCCATGTGGTATAAGATTATTCTCCCCCTTGTATTCTTTCGAGTTCCGTCGTGTTTTGTCCGATAAGAAAGCGTTGGATGTCACGACTCTTCTTTCTTTGATTGAGGAGTTTGAGTTCAGTTTGGGAGCTTTTTTTCGCCTCTTTTGGGAGACATCCGATGGTCTTTCAGGACGTGAGAGCGATGATCGAAGAGTTCCTTCTCGATCCGATTTACTGAGGCAAAGGAGGATTCTTGCGGCTAGCCGACATAAACTCTTCAAAGTTGATGAGCTAGCTTTGTATAATGGAACTGATGAGGGATTGCCTATTCTTCTGGGAATTCTTGGATCAGTATTTGATGTCACAAAAGGAAAATCTCACTATGGTGTGGGAGGAGGTTATCATCACTTTGCTGGAAGAGATGCTTCTCGTGCATTTGTATCAGGAAATTTCACAGGGGATGGACTCACCGATTCTTTGCGGGGTTTGTCAAATACTGAGATCAAGAGTGTTGTTGAATGGCGGAGCTTTTACCATAAAACCTACACATTTGTAGGTAAGTTGGTGGGTCTTTACTATGATGATCAAGGAAATCCCACTAAACATTTAAAGGGAGCTGAGGCAAAGGCTGCTAGAGGCGCACAACTGTTGAAGAAACAAAAGAAAGAGGAAGATAAACTGCCAAGTTGTAATTCAAAATGGAGTCAGGCAGAAGGTGGTGAGGTCTGGTGTGATGATGGCTTCCCGAGGTTGGTACAGAGGCCATTAGAAATTGCATTAACAGGGAAGATGAGGAAACGATGCGCTTGCTTTAGAGAAGACCAACTGAGTGAGCCGGGATTGGAAGTCTATGAAGGTTGTGACTACCTAGCCAAGACTTGCCGTGTTTAA

Coding sequence (CDS)

ATGGCCAGTGATCTCTCTCCGCAAAGACCAAGCGAAGACGCGCATGAACAGAAGAGGCCGAGCTCGGTGAAGGGCATAGCTATGGCGGGAAAGATCACGGGGTGGGTTTTGGCTCTTATGGTGGCTATTGTTGCGCTTCTCATTGGGTTTTCCTTCAAATCCCGATACCCGATCTCCTTCTCCTCCCCCTCCCCTGAACAGCTTGATAAATGGGAGAAATCTACTCTCTCCATGGGGGATGGGAAAGCTCCATGTGGTATAAGATTATTCTCCCCCTTGTATTCTTTCGAGTTCCGTCGTGTTTTGTCCGATAAGAAAGCGTTGGATGTCACGACTCTTCTTTCTTTGATTGAGGAGTTTGAGTTCAGTTTGGGAGCTTTTTTTCGCCTCTTTTGGGAGACATCCGATGGTCTTTCAGGACGTGAGAGCGATGATCGAAGAGTTCCTTCTCGATCCGATTTACTGAGGCAAAGGAGGATTCTTGCGGCTAGCCGACATAAACTCTTCAAAGTTGATGAGCTAGCTTTGTATAATGGAACTGATGAGGGATTGCCTATTCTTCTGGGAATTCTTGGATCAGTATTTGATGTCACAAAAGGAAAATCTCACTATGGTGTGGGAGGAGGTTATCATCACTTTGCTGGAAGAGATGCTTCTCGTGCATTTGTATCAGGAAATTTCACAGGGGATGGACTCACCGATTCTTTGCGGGGTTTGTCAAATACTGAGATCAAGAGTGTTGTTGAATGGCGGAGCTTTTACCATAAAACCTACACATTTGTAGGTAAGTTGGTGGGTCTTTACTATGATGATCAAGGAAATCCCACTAAACATTTAAAGGGAGCTGAGGCAAAGGCTGCTAGAGGCGCACAACTGTTGAAGAAACAAAAGAAAGAGGAAGATAAACTGCCAAGTTGTAATTCAAAATGGAGTCAGGCAGAAGGTGGTGAGGTCTGGTGTGATGATGGCTTCCCGAGGTTGGTACAGAGGCCATTAGAAATTGCATTAACAGGGAAGATGAGGAAACGATGCGCTTGCTTTAGAGAAGACCAACTGAGTGAGCCGGGATTGGAAGTCTATGAAGGTTGTGACTACCTAGCCAAGACTTGCCGTGTTTAA

Protein sequence

MASDLSPQRPSEDAHEQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPISFSSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIEEFEFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLEVYEGCDYLAKTCRV
Homology
BLAST of Spg016300 vs. NCBI nr
Match: XP_022981227.1 (membrane-associated progesterone-binding protein 4 [Cucurbita maxima])

HSP 1 Score: 456.8 bits (1174), Expect = 1.7e-124
Identity = 245/374 (65.51%), Postives = 256/374 (68.45%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAH--EQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPI 60
           MASD SPQRP EDAH  EQK  SSVKG AMAGK  GWVLAL+VA+VAL +G SFKSRYPI
Sbjct: 1   MASDQSPQRPGEDAHEDEQKGSSSVKGKAMAGKTMGWVLALIVAVVALFVGLSFKSRYPI 60

Query: 61  SFSSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIE 120
           + S PSP+Q                                                   
Sbjct: 61  APSFPSPQQ--------------------------------------------------- 120

Query: 121 EFEFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYN 180
                                                           KLFKVDELALYN
Sbjct: 121 ------------------------------------------------KLFKVDELALYN 180

Query: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRG 240
           GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHF+GRDASRAFVSGNFTG+GLTDSLRG
Sbjct: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFSGRDASRAFVSGNFTGEGLTDSLRG 240

Query: 241 LSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 300
           LSN EIKSVVEWR FYHK+YTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK
Sbjct: 241 LSNAEIKSVVEWRGFYHKSYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 275

Query: 301 EEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLE 360
           EEDKLPSCNSKWSQ EGGEVWCD+GFPRL+QRPLEIALTGKM KRCACFREDQL EPGLE
Sbjct: 301 EEDKLPSCNSKWSQDEGGEVWCDNGFPRLIQRPLEIALTGKMSKRCACFREDQLGEPGLE 275

Query: 361 VYEGCDYLAKTCRV 373
           VYEGCDY A+TCRV
Sbjct: 361 VYEGCDYQAQTCRV 275

BLAST of Spg016300 vs. NCBI nr
Match: XP_022940087.1 (membrane-associated progesterone-binding protein 4 [Cucurbita moschata])

HSP 1 Score: 453.4 bits (1165), Expect = 1.9e-123
Identity = 244/374 (65.24%), Postives = 254/374 (67.91%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAH--EQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPI 60
           MASD SP RP EDAH  EQK  SSVKG AMAGK  GWVLAL+VA+VAL +G SFKSRYPI
Sbjct: 1   MASDQSPPRPGEDAHEDEQKGSSSVKGKAMAGKTLGWVLALIVAVVALFVGLSFKSRYPI 60

Query: 61  SFSSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIE 120
           + S PSP+                                                    
Sbjct: 61  APSLPSPQL--------------------------------------------------- 120

Query: 121 EFEFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYN 180
                                                           KLFKVDELALYN
Sbjct: 121 ------------------------------------------------KLFKVDELALYN 180

Query: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRG 240
           GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHF+GRDASRAFVSGNFTG+GLTDSLRG
Sbjct: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFSGRDASRAFVSGNFTGEGLTDSLRG 240

Query: 241 LSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 300
           LSN EIKSVVEWR FYHK+YTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK
Sbjct: 241 LSNAEIKSVVEWRGFYHKSYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 275

Query: 301 EEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLE 360
           EEDKLPSCNSKWSQ EGGEVWCD+GFPRL+QRPLEIALTGKM KRCACFREDQL EPGLE
Sbjct: 301 EEDKLPSCNSKWSQDEGGEVWCDNGFPRLIQRPLEIALTGKMSKRCACFREDQLGEPGLE 275

Query: 361 VYEGCDYLAKTCRV 373
           VYEGCDY AKTCRV
Sbjct: 361 VYEGCDYQAKTCRV 275

BLAST of Spg016300 vs. NCBI nr
Match: XP_023524767.1 (membrane-associated progesterone-binding protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 449.9 bits (1156), Expect = 2.1e-122
Identity = 243/374 (64.97%), Postives = 253/374 (67.65%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAH--EQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPI 60
           MASD SP RP EDAH  EQK  SSVKG AMAGK  GWVLAL+VA+VAL +G SFKSRY I
Sbjct: 1   MASDQSPPRPGEDAHEDEQKGSSSVKGKAMAGKTLGWVLALIVAVVALFVGLSFKSRYRI 60

Query: 61  SFSSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIE 120
           + S PSP+                                                    
Sbjct: 61  APSFPSPQL--------------------------------------------------- 120

Query: 121 EFEFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYN 180
                                                           KLFKVDELALYN
Sbjct: 121 ------------------------------------------------KLFKVDELALYN 180

Query: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRG 240
           GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHF+GRDASRAFVSGNFTG+GLTDSLRG
Sbjct: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFSGRDASRAFVSGNFTGEGLTDSLRG 240

Query: 241 LSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 300
           LSN EIKSVVEWR FYHK+YTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK
Sbjct: 241 LSNAEIKSVVEWRGFYHKSYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 275

Query: 301 EEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLE 360
           EEDKLPSCNSKWSQ EGGEVWCD+GFPRL+QRPLEIALTGKM KRCACFREDQL EPGLE
Sbjct: 301 EEDKLPSCNSKWSQDEGGEVWCDNGFPRLIQRPLEIALTGKMSKRCACFREDQLGEPGLE 275

Query: 361 VYEGCDYLAKTCRV 373
           VYEGCDY AKTCRV
Sbjct: 361 VYEGCDYQAKTCRV 275

BLAST of Spg016300 vs. NCBI nr
Match: KAA0063359.1 (membrane-associated progesterone-binding protein 4 [Cucumis melo var. makuwa] >TYK28061.1 membrane-associated progesterone-binding protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 440.7 bits (1132), Expect = 1.3e-119
Identity = 239/372 (64.25%), Postives = 248/372 (66.67%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAHEQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPISF 60
           MASDLS Q+PSE+AH     SSVKG  M GKI GWVLA +VAI AL IG SFKSRY IS 
Sbjct: 78  MASDLSQQKPSEEAHAHGSSSSVKGKEMGGKIRGWVLASIVAIFALFIGVSFKSRYKISS 137

Query: 61  SSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIEEF 120
            SPS +Q                                                     
Sbjct: 138 ISPSAQQ----------------------------------------------------- 197

Query: 121 EFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYNGT 180
                                                         KLFKVDELALYNGT
Sbjct: 198 ----------------------------------------------KLFKVDELALYNGT 257

Query: 181 DEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRGLS 240
           D+GLPILLGILGSVFDVTKGKSHYGVGGGY+HFAGRDASRAFVSGNFTG+GLTDSLRGLS
Sbjct: 258 DKGLPILLGILGSVFDVTKGKSHYGVGGGYNHFAGRDASRAFVSGNFTGEGLTDSLRGLS 317

Query: 241 NTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 300
           N EIKSVVEWRSFY KTYT VGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE
Sbjct: 318 NAEIKSVVEWRSFYQKTYTLVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 350

Query: 301 DKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLEVY 360
           DKLPSCNS+WSQ EGGEVWCDDGFPRLVQRPLEIALTGKM KRCACFREDQL EPGLEVY
Sbjct: 378 DKLPSCNSRWSQGEGGEVWCDDGFPRLVQRPLEIALTGKMSKRCACFREDQLDEPGLEVY 350

Query: 361 EGCDYLAKTCRV 373
           +GCDY AKTCRV
Sbjct: 438 DGCDYQAKTCRV 350

BLAST of Spg016300 vs. NCBI nr
Match: XP_008464659.1 (PREDICTED: membrane-associated progesterone-binding protein 4 [Cucumis melo])

HSP 1 Score: 440.7 bits (1132), Expect = 1.3e-119
Identity = 239/372 (64.25%), Postives = 248/372 (66.67%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAHEQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPISF 60
           MASDLS Q+PSE+AH     SSVKG  M GKI GWVLA +VAI AL IG SFKSRY IS 
Sbjct: 1   MASDLSQQKPSEEAHAHGSSSSVKGKEMGGKIRGWVLASIVAIFALFIGVSFKSRYKISS 60

Query: 61  SSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIEEF 120
            SPS +Q                                                     
Sbjct: 61  ISPSAQQ----------------------------------------------------- 120

Query: 121 EFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYNGT 180
                                                         KLFKVDELALYNGT
Sbjct: 121 ----------------------------------------------KLFKVDELALYNGT 180

Query: 181 DEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRGLS 240
           D+GLPILLGILGSVFDVTKGKSHYGVGGGY+HFAGRDASRAFVSGNFTG+GLTDSLRGLS
Sbjct: 181 DKGLPILLGILGSVFDVTKGKSHYGVGGGYNHFAGRDASRAFVSGNFTGEGLTDSLRGLS 240

Query: 241 NTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 300
           N EIKSVVEWRSFY KTYT VGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE
Sbjct: 241 NAEIKSVVEWRSFYQKTYTLVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 273

Query: 301 DKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLEVY 360
           DKLPSCNS+WSQ EGGEVWCDDGFPRLVQRPLEIALTGKM KRCACFREDQL EPGLEVY
Sbjct: 301 DKLPSCNSRWSQGEGGEVWCDDGFPRLVQRPLEIALTGKMSKRCACFREDQLDEPGLEVY 273

Query: 361 EGCDYLAKTCRV 373
           +GCDY AKTCRV
Sbjct: 361 DGCDYQAKTCRV 273

BLAST of Spg016300 vs. ExPASy Swiss-Prot
Match: Q2HIW2 (Membrane-associated progesterone-binding protein 4 OS=Arabidopsis thaliana OX=3702 GN=MAPR4 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.7e-93
Identity = 162/206 (78.64%), Postives = 182/206 (88.35%), Query Frame = 0

Query: 167 KLFKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGN 226
           +LF  +ELALYNGTDE LPILLGILGSVFDVTKGK HYG GGGY+HFAGRDASRAFVSGN
Sbjct: 40  RLFSAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGN 99

Query: 227 FTGDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKA 286
           FTGDGLTDSL+GLS++E+KS+V+WR FY +TYT VGKLVG YYD QGNPTKHLKGAEAKA
Sbjct: 100 FTGDGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLVGRYYDSQGNPTKHLKGAEAKA 159

Query: 287 ARGAQLLKKQKKEEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCAC 346
           +RGAQL++KQK EE K  +CNS+WSQ EGGEVWCD G PRLVQRPLEIA+TG M KRCAC
Sbjct: 160 SRGAQLMEKQKTEEAKQSNCNSRWSQDEGGEVWCDVGVPRLVQRPLEIAITGSMSKRCAC 219

Query: 347 FREDQLSEPGLEVYEGCDYLAKTCRV 373
           F EDQL + GLE+Y+ C+ LAKTCRV
Sbjct: 220 FEEDQLDQSGLEIYKDCEPLAKTCRV 245

BLAST of Spg016300 vs. ExPASy Swiss-Prot
Match: A2CES0 (Neuferricin OS=Danio rerio OX=7955 GN=cyb5d2 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 4.3e-41
Identity = 95/236 (40.25%), Postives = 131/236 (55.51%), Query Frame = 0

Query: 155 LRQR--RILAASRHKLFKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHH 214
           LRQR   + A+S   L   ++L+LYNG      + L ILG VFDV KG+ HYG GGGYH 
Sbjct: 38  LRQRVSEVSASSPGLLLTKEQLSLYNGGKNSKGLYLAILGQVFDVEKGRKHYGPGGGYHF 97

Query: 215 FAGRDASRAFVSGNFTGDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQ 274
           F G+DASRAF++G+FT  GL++ +   S ++I ++ +W SFY + YT VGKL+G +Y + 
Sbjct: 98  FTGKDASRAFITGDFTEAGLSNDVSDFSESQIVALYDWLSFYQRDYTPVGKLIGRFYTET 157

Query: 275 GNPTKHLKGAEAKAARGAQLLKKQKKEEDKLPSCNSKWSQAEGGEVWCDD---------- 334
           G PT  L   EA  + G +   + + E    PSCNS+WS+A GG VWC            
Sbjct: 158 GQPTDALLHVEAFLSDGLKKKAQAQSEMQLYPSCNSEWSEASGGRVWCSTMSGGIHRDWV 217

Query: 335 GFPRLVQRPLEIALTGKMRKRCACFREDQLSEP------GLEVYEGCDYLAKTCRV 373
           G PR++  P      G    RC C R   LS+P       L  Y  C   A++C++
Sbjct: 218 GVPRMLFTP------GSGHSRCVCIR---LSDPVHSENRNLREYTDCPPRAESCQI 264

BLAST of Spg016300 vs. ExPASy Swiss-Prot
Match: Q5SSH8 (Neuferricin OS=Mus musculus OX=10090 GN=Cyb5d2 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 8.1e-40
Identity = 91/230 (39.57%), Postives = 122/230 (53.04%), Query Frame = 0

Query: 167 KLFKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGN 226
           +LF  +ELA Y G      + L +LG V+DV+ G+ HY  G  Y  FAGRDASRAFV+G+
Sbjct: 36  RLFLPEELARYRGGPGDPGLYLALLGRVYDVSSGRRHYEPGAHYSGFAGRDASRAFVTGD 95

Query: 227 FTGDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKA 286
           ++  GL D + GLS++EI ++  W SFY K Y FVG+LVG +Y   G PT  L   EA  
Sbjct: 96  YSEAGLVDDINGLSSSEILTLHNWLSFYEKNYVFVGRLVGRFYRKDGLPTSELTQVEAMV 155

Query: 287 ARGAQLLKKQKKEEDKLPSCNSKWSQAEGGEVWCDD----------GFPRLVQRPLEIAL 346
            +G +  +++++E+ K P CNS+WS A+G  +WC            G PR + +P     
Sbjct: 156 TKGMEANEQEQREKQKFPPCNSEWSSAKGSRLWCSQKSGGVHRDWIGVPRKLYKP----- 215

Query: 347 TGKMRKRCACFR-----EDQ-----------LSEPGLEVYEGCDYLAKTC 371
            G     C C R      DQ           L  P LE Y GC  LA TC
Sbjct: 216 -GAKEPHCVCVRTTGPPSDQQDNPRHSNHGDLDNPNLEEYTGCPPLATTC 259

BLAST of Spg016300 vs. ExPASy Swiss-Prot
Match: Q6AY62 (Neuferricin OS=Rattus norvegicus OX=10116 GN=Cyb5d2 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 5.2e-39
Identity = 88/230 (38.26%), Postives = 122/230 (53.04%), Query Frame = 0

Query: 167 KLFKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGN 226
           +LF  +ELA Y G      + L +LG V+DV+ G+ HY  G  Y  FAGRDASRAFV+G+
Sbjct: 36  RLFVPEELARYRGGPGDPGLYLALLGRVYDVSSGRKHYEPGAHYSGFAGRDASRAFVTGD 95

Query: 227 FTGDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKA 286
           ++  GL D + GLS++EI ++  W SFY K Y FVG+L+G +Y   G PT  L   EA  
Sbjct: 96  YSEAGLVDDVNGLSSSEILTLHNWLSFYEKNYVFVGRLIGRFYGKDGLPTSELTQVEAMV 155

Query: 287 ARGAQLLKKQKKEEDKLPSCNSKWSQAEGGEVWCDD----------GFPRLVQRPLEIAL 346
            +G +  +++++E+ + P CN++WS A+G  +WC            G PR + +P     
Sbjct: 156 TKGMEANEQEQREKQRFPPCNAEWSSAKGSRLWCSQKSGGVHRDWIGVPRKLYKP----- 215

Query: 347 TGKMRKRCACFR-----EDQ-----------LSEPGLEVYEGCDYLAKTC 371
            G     C C R      DQ           L  P LE Y GC  LA TC
Sbjct: 216 -GAKEPHCVCVRTTGPPSDQQDNPRHSNRGDLDNPNLEEYTGCPPLAVTC 259

BLAST of Spg016300 vs. ExPASy Swiss-Prot
Match: Q28FI8 (Neuferricin OS=Xenopus tropicalis OX=8364 GN=cyb5d2 PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 6.0e-35
Identity = 87/227 (38.33%), Postives = 119/227 (52.42%), Query Frame = 0

Query: 164 SRHKLFKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFV 223
           S  +L   +EL++Y+G      I L ILG VFDV KG  HYG GG Y  FAG+DASRA++
Sbjct: 42  SEGRLMSKEELSVYDGGPGSSGIYLAILGQVFDVHKGSKHYGPGGSYSFFAGKDASRAYM 101

Query: 224 SGNFTGDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAE 283
           +G+FT  GL D +  LS  ++  +  W SFY + Y  +GKL G +YD+ GNPTK L+ A 
Sbjct: 102 TGDFTEKGLVDDVTELSPLQMLHLHNWLSFYQQNYITIGKLTGRFYDESGNPTKALEDAL 161

Query: 284 AKAARGAQLLKKQKKEEDKLPSCNSKWSQAEGGEVWC---DDGFPR-LVQRPLEIALTGK 343
                G +L +++++E  + P CNS+WS +E   VWC     G  R  V  P ++   G 
Sbjct: 162 KVIDIGLKLKEEREEENKQFPPCNSEWS-SESKRVWCSKNSGGIQRDWVGVPRKMYTAGT 221

Query: 344 MRKRCACFR----------------EDQLSEPGLEVYEGCDYLAKTC 371
              RC C R                   L  P L  YE C+ L + C
Sbjct: 222 NGYRCVCVRNFGPPSEQPDSTEHNDRGDLDNPMLHEYEDCNPLFEWC 267

BLAST of Spg016300 vs. ExPASy TrEMBL
Match: A0A6J1ITE8 (membrane-associated progesterone-binding protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111480426 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 8.5e-125
Identity = 245/374 (65.51%), Postives = 256/374 (68.45%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAH--EQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPI 60
           MASD SPQRP EDAH  EQK  SSVKG AMAGK  GWVLAL+VA+VAL +G SFKSRYPI
Sbjct: 1   MASDQSPQRPGEDAHEDEQKGSSSVKGKAMAGKTMGWVLALIVAVVALFVGLSFKSRYPI 60

Query: 61  SFSSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIE 120
           + S PSP+Q                                                   
Sbjct: 61  APSFPSPQQ--------------------------------------------------- 120

Query: 121 EFEFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYN 180
                                                           KLFKVDELALYN
Sbjct: 121 ------------------------------------------------KLFKVDELALYN 180

Query: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRG 240
           GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHF+GRDASRAFVSGNFTG+GLTDSLRG
Sbjct: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFSGRDASRAFVSGNFTGEGLTDSLRG 240

Query: 241 LSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 300
           LSN EIKSVVEWR FYHK+YTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK
Sbjct: 241 LSNAEIKSVVEWRGFYHKSYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 275

Query: 301 EEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLE 360
           EEDKLPSCNSKWSQ EGGEVWCD+GFPRL+QRPLEIALTGKM KRCACFREDQL EPGLE
Sbjct: 301 EEDKLPSCNSKWSQDEGGEVWCDNGFPRLIQRPLEIALTGKMSKRCACFREDQLGEPGLE 275

Query: 361 VYEGCDYLAKTCRV 373
           VYEGCDY A+TCRV
Sbjct: 361 VYEGCDYQAQTCRV 275

BLAST of Spg016300 vs. ExPASy TrEMBL
Match: A0A6J1FIN0 (membrane-associated progesterone-binding protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111445811 PE=4 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 9.3e-124
Identity = 244/374 (65.24%), Postives = 254/374 (67.91%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAH--EQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPI 60
           MASD SP RP EDAH  EQK  SSVKG AMAGK  GWVLAL+VA+VAL +G SFKSRYPI
Sbjct: 1   MASDQSPPRPGEDAHEDEQKGSSSVKGKAMAGKTLGWVLALIVAVVALFVGLSFKSRYPI 60

Query: 61  SFSSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIE 120
           + S PSP+                                                    
Sbjct: 61  APSLPSPQL--------------------------------------------------- 120

Query: 121 EFEFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYN 180
                                                           KLFKVDELALYN
Sbjct: 121 ------------------------------------------------KLFKVDELALYN 180

Query: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRG 240
           GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHF+GRDASRAFVSGNFTG+GLTDSLRG
Sbjct: 181 GTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFSGRDASRAFVSGNFTGEGLTDSLRG 240

Query: 241 LSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 300
           LSN EIKSVVEWR FYHK+YTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK
Sbjct: 241 LSNAEIKSVVEWRGFYHKSYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKK 275

Query: 301 EEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLE 360
           EEDKLPSCNSKWSQ EGGEVWCD+GFPRL+QRPLEIALTGKM KRCACFREDQL EPGLE
Sbjct: 301 EEDKLPSCNSKWSQDEGGEVWCDNGFPRLIQRPLEIALTGKMSKRCACFREDQLGEPGLE 275

Query: 361 VYEGCDYLAKTCRV 373
           VYEGCDY AKTCRV
Sbjct: 361 VYEGCDYQAKTCRV 275

BLAST of Spg016300 vs. ExPASy TrEMBL
Match: A0A5A7VCP1 (Membrane-associated progesterone-binding protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G003220 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 6.3e-120
Identity = 239/372 (64.25%), Postives = 248/372 (66.67%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAHEQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPISF 60
           MASDLS Q+PSE+AH     SSVKG  M GKI GWVLA +VAI AL IG SFKSRY IS 
Sbjct: 78  MASDLSQQKPSEEAHAHGSSSSVKGKEMGGKIRGWVLASIVAIFALFIGVSFKSRYKISS 137

Query: 61  SSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIEEF 120
            SPS +Q                                                     
Sbjct: 138 ISPSAQQ----------------------------------------------------- 197

Query: 121 EFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYNGT 180
                                                         KLFKVDELALYNGT
Sbjct: 198 ----------------------------------------------KLFKVDELALYNGT 257

Query: 181 DEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRGLS 240
           D+GLPILLGILGSVFDVTKGKSHYGVGGGY+HFAGRDASRAFVSGNFTG+GLTDSLRGLS
Sbjct: 258 DKGLPILLGILGSVFDVTKGKSHYGVGGGYNHFAGRDASRAFVSGNFTGEGLTDSLRGLS 317

Query: 241 NTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 300
           N EIKSVVEWRSFY KTYT VGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE
Sbjct: 318 NAEIKSVVEWRSFYQKTYTLVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 350

Query: 301 DKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLEVY 360
           DKLPSCNS+WSQ EGGEVWCDDGFPRLVQRPLEIALTGKM KRCACFREDQL EPGLEVY
Sbjct: 378 DKLPSCNSRWSQGEGGEVWCDDGFPRLVQRPLEIALTGKMSKRCACFREDQLDEPGLEVY 350

Query: 361 EGCDYLAKTCRV 373
           +GCDY AKTCRV
Sbjct: 438 DGCDYQAKTCRV 350

BLAST of Spg016300 vs. ExPASy TrEMBL
Match: A0A1S3CMH5 (membrane-associated progesterone-binding protein 4 OS=Cucumis melo OX=3656 GN=LOC103502493 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 6.3e-120
Identity = 239/372 (64.25%), Postives = 248/372 (66.67%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAHEQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPISF 60
           MASDLS Q+PSE+AH     SSVKG  M GKI GWVLA +VAI AL IG SFKSRY IS 
Sbjct: 1   MASDLSQQKPSEEAHAHGSSSSVKGKEMGGKIRGWVLASIVAIFALFIGVSFKSRYKISS 60

Query: 61  SSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIEEF 120
            SPS +Q                                                     
Sbjct: 61  ISPSAQQ----------------------------------------------------- 120

Query: 121 EFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYNGT 180
                                                         KLFKVDELALYNGT
Sbjct: 121 ----------------------------------------------KLFKVDELALYNGT 180

Query: 181 DEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRGLS 240
           D+GLPILLGILGSVFDVTKGKSHYGVGGGY+HFAGRDASRAFVSGNFTG+GLTDSLRGLS
Sbjct: 181 DKGLPILLGILGSVFDVTKGKSHYGVGGGYNHFAGRDASRAFVSGNFTGEGLTDSLRGLS 240

Query: 241 NTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 300
           N EIKSVVEWRSFY KTYT VGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE
Sbjct: 241 NAEIKSVVEWRSFYQKTYTLVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 273

Query: 301 DKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLEVY 360
           DKLPSCNS+WSQ EGGEVWCDDGFPRLVQRPLEIALTGKM KRCACFREDQL EPGLEVY
Sbjct: 301 DKLPSCNSRWSQGEGGEVWCDDGFPRLVQRPLEIALTGKMSKRCACFREDQLDEPGLEVY 273

Query: 361 EGCDYLAKTCRV 373
           +GCDY AKTCRV
Sbjct: 361 DGCDYQAKTCRV 273

BLAST of Spg016300 vs. ExPASy TrEMBL
Match: A0A0A0KZ74 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G617330 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 3.1e-119
Identity = 237/372 (63.71%), Postives = 248/372 (66.67%), Query Frame = 0

Query: 1   MASDLSPQRPSEDAHEQKRPSSVKGIAMAGKITGWVLALMVAIVALLIGFSFKSRYPISF 60
           MA DLS Q+ SEDAH  ++ SSVKG  M GKI GWVLA +VAI AL IGFSFKSRY IS 
Sbjct: 1   MAGDLSQQKASEDAHAHEKSSSVKGKEMRGKIMGWVLASIVAIFALFIGFSFKSRYQISS 60

Query: 61  SSPSPEQLDKWEKSTLSMGDGKAPCGIRLFSPLYSFEFRRVLSDKKALDVTTLLSLIEEF 120
            SPS +Q                                                     
Sbjct: 61  ISPSSQQ----------------------------------------------------- 120

Query: 121 EFSLGAFFRLFWETSDGLSGRESDDRRVPSRSDLLRQRRILAASRHKLFKVDELALYNGT 180
                                                         KLFKVDELALYNGT
Sbjct: 121 ----------------------------------------------KLFKVDELALYNGT 180

Query: 181 DEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDGLTDSLRGLS 240
           D+GLPILLGILGSVFDVTKGKSHYGVGGGY+HFAGRDASRAFVSGNF G+GLTDSLRGLS
Sbjct: 181 DKGLPILLGILGSVFDVTKGKSHYGVGGGYNHFAGRDASRAFVSGNFAGEGLTDSLRGLS 240

Query: 241 NTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 300
           N EIKSVVEWRSFY KTYT VGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE
Sbjct: 241 NAEIKSVVEWRSFYQKTYTLVGKLVGLYYDDQGNPTKHLKGAEAKAARGAQLLKKQKKEE 273

Query: 301 DKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCACFREDQLSEPGLEVY 360
           DKLPSCNS+WSQ EGGEVWCD+GFPRLVQRPLEIALTGKM KRCACFREDQL EPGLEVY
Sbjct: 301 DKLPSCNSRWSQGEGGEVWCDNGFPRLVQRPLEIALTGKMSKRCACFREDQLGEPGLEVY 273

Query: 361 EGCDYLAKTCRV 373
           +GCDY AKTCRV
Sbjct: 361 DGCDYQAKTCRV 273

BLAST of Spg016300 vs. TAIR 10
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4 )

HSP 1 Score: 344.4 bits (882), Expect = 1.2e-94
Identity = 162/206 (78.64%), Postives = 182/206 (88.35%), Query Frame = 0

Query: 167 KLFKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGN 226
           +LF  +ELALYNGTDE LPILLGILGSVFDVTKGK HYG GGGY+HFAGRDASRAFVSGN
Sbjct: 40  RLFSAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGN 99

Query: 227 FTGDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKA 286
           FTGDGLTDSL+GLS++E+KS+V+WR FY +TYT VGKLVG YYD QGNPTKHLKGAEAKA
Sbjct: 100 FTGDGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLVGRYYDSQGNPTKHLKGAEAKA 159

Query: 287 ARGAQLLKKQKKEEDKLPSCNSKWSQAEGGEVWCDDGFPRLVQRPLEIALTGKMRKRCAC 346
           +RGAQL++KQK EE K  +CNS+WSQ EGGEVWCD G PRLVQRPLEIA+TG M KRCAC
Sbjct: 160 SRGAQLMEKQKTEEAKQSNCNSRWSQDEGGEVWCDVGVPRLVQRPLEIAITGSMSKRCAC 219

Query: 347 FREDQLSEPGLEVYEGCDYLAKTCRV 373
           F EDQL + GLE+Y+ C+ LAKTCRV
Sbjct: 220 FEEDQLDQSGLEIYKDCEPLAKTCRV 245

BLAST of Spg016300 vs. TAIR 10
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2 )

HSP 1 Score: 89.0 bits (219), Expect = 8.9e-18
Identity = 44/97 (45.36%), Postives = 61/97 (62.89%), Query Frame = 0

Query: 169 FKVDELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFT 228
           F  ++L+ YNGTDE  PI + I G VFDVT GKS YG GG Y  FAG+DASRA    +  
Sbjct: 3   FTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKN 62

Query: 229 GDGLTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLV 266
            + ++ SL GL+  EI ++ +W + +   Y  VG++V
Sbjct: 63  EEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVV 99

BLAST of Spg016300 vs. TAIR 10
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3 )

HSP 1 Score: 75.1 bits (183), Expect = 1.3e-13
Identity = 40/118 (33.90%), Postives = 64/118 (54.24%), Query Frame = 0

Query: 172 DELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDG 231
           +EL LY+G+D   P+L+ I G ++DV++ +  YG GG Y  FAG+DASRA    +F    
Sbjct: 74  EELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEDQD 133

Query: 232 LTDSLRGLSNTEIKSVVEWRSFYHKTYTFVGKLVGLYYDDQGNPTKHLKGAEAKAARG 290
           LT  + GL   E++++ +W   +   Y  VG +     D +G  +     A+  +A G
Sbjct: 134 LTGDISGLGAFELEALQDWEYKFMSKYVKVGTI--QKKDGEGKESSEPSEAKTASAEG 189

BLAST of Spg016300 vs. TAIR 10
Match: AT5G52240.2 (membrane steroid binding protein 1 )

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-11
Identity = 37/98 (37.76%), Postives = 55/98 (56.12%), Query Frame = 0

Query: 172 DELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDG 231
           +EL  Y+G+D   P+L+ I   ++DVT+ +  YG GG Y  FAG+DASRA    +F    
Sbjct: 78  EELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDASRALAKMSFEEKD 137

Query: 232 LTDSLRGLSNTEIKSVVEWRSFYHKTYTFVG--KLVGL 268
           LT  + GL   E+ ++ +W   +   Y  VG  K+ GL
Sbjct: 138 LTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGL 175

BLAST of Spg016300 vs. TAIR 10
Match: AT5G52240.1 (membrane steroid binding protein 1 )

HSP 1 Score: 66.6 bits (161), Expect = 4.7e-11
Identity = 34/91 (37.36%), Postives = 51/91 (56.04%), Query Frame = 0

Query: 172 DELALYNGTDEGLPILLGILGSVFDVTKGKSHYGVGGGYHHFAGRDASRAFVSGNFTGDG 231
           +EL  Y+G+D   P+L+ I   ++DVT+ +  YG GG Y  FAG+DASRA    +F    
Sbjct: 78  EELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFAGKDASRALAKMSFEEKD 137

Query: 232 LTDSLRGLSNTEIKSVVEWRSFYHKTYTFVG 263
           LT  + GL   E+ ++ +W   +   Y  VG
Sbjct: 138 LTWDVSGLGPFELDALQDWEYKFMSKYAKVG 168

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022981227.11.7e-12465.51membrane-associated progesterone-binding protein 4 [Cucurbita maxima][more]
XP_022940087.11.9e-12365.24membrane-associated progesterone-binding protein 4 [Cucurbita moschata][more]
XP_023524767.12.1e-12264.97membrane-associated progesterone-binding protein 4 [Cucurbita pepo subsp. pepo][more]
KAA0063359.11.3e-11964.25membrane-associated progesterone-binding protein 4 [Cucumis melo var. makuwa] >T... [more]
XP_008464659.11.3e-11964.25PREDICTED: membrane-associated progesterone-binding protein 4 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q2HIW21.7e-9378.64Membrane-associated progesterone-binding protein 4 OS=Arabidopsis thaliana OX=37... [more]
A2CES04.3e-4140.25Neuferricin OS=Danio rerio OX=7955 GN=cyb5d2 PE=3 SV=1[more]
Q5SSH88.1e-4039.57Neuferricin OS=Mus musculus OX=10090 GN=Cyb5d2 PE=1 SV=1[more]
Q6AY625.2e-3938.26Neuferricin OS=Rattus norvegicus OX=10116 GN=Cyb5d2 PE=2 SV=1[more]
Q28FI86.0e-3538.33Neuferricin OS=Xenopus tropicalis OX=8364 GN=cyb5d2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1ITE88.5e-12565.51membrane-associated progesterone-binding protein 4 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1FIN09.3e-12465.24membrane-associated progesterone-binding protein 4 OS=Cucurbita moschata OX=3662... [more]
A0A5A7VCP16.3e-12064.25Membrane-associated progesterone-binding protein 4 OS=Cucumis melo var. makuwa O... [more]
A0A1S3CMH56.3e-12064.25membrane-associated progesterone-binding protein 4 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A0A0KZ743.1e-11963.71Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ... [more]
Match NameE-valueIdentityDescription
AT4G14965.11.2e-9478.64membrane-associated progesterone binding protein 4 [more]
AT2G24940.18.9e-1845.36membrane-associated progesterone binding protein 2 [more]
AT3G48890.11.3e-1333.90membrane-associated progesterone binding protein 3 [more]
AT5G52240.21.6e-1137.76membrane steroid binding protein 1 [more]
AT5G52240.14.7e-1137.36membrane steroid binding protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 169..265
e-value: 4.3E-21
score: 86.1
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 172..232
e-value: 4.0E-9
score: 36.5
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 161..266
e-value: 2.4E-32
score: 113.3
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 164..265
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR10281MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATEDcoord: 159..367
NoneNo IPR availablePANTHERPTHR10281:SF4NEUFERRICINcoord: 159..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016300.1Spg016300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane