Spg016276 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016276
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionTranscription initiation factor IIA subunit 2
Locationscaffold9: 41904203 .. 41906120 (+)
RNA-Seq ExpressionSpg016276
SyntenySpg016276
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTTTCTGTGCTCTTCGAGGGAATTGCTTTCGTACTTTGTTGGCGGGAAAGGTCGCCTAAGAAAATCGGTCAAATCTTTGACTTTATTACTCGGTACCTAACCGTTTCTATTTTGGGCTGGGCCGAAATAGGGTTTTAATGGGCTCGAGAACAAACTGGGCCTTCAAATCATAGGCTTAACGGCCCATTTTACTTTTTGAGCCCAAAATAAGAAACGAAGTCGCCTCGCTAGGGGCAAAATCGTCATTTCGACGAGTTTCTATTCCGTCGCTTGGCTGCTATATATACCCTCATCTACAAAATCTAGGGTATAAAAAGCCCCAAATTTCTTCGCTCGCGAAAAGCTTTGGAGCTAACGTTAACCCAGTTAGAATCAAGAAACGAGAAGAATCACAAACTCACCAACAATCTGTAGCTTCAATTCCTTCCTTGTACTCTCTCTTTATTATTATTATTAATTTTCATTCCTTTTCGAATTGATCTCTCGCTTTAATTTCGAAGATTTTGCCAGCTTTCTCGAATTTTGACGCTATGTTCCAAATTTTTTTGTAGTTTTATCAATTCTGGATGTGTATTTTTGAACTAAATTTGATATCTTAGCTAAAGAACTTCCATATTTAGCAGATCTTGTTTTTCGAAGAGGAATGGCAACTTTTGAACTCTATCGCAGATCGACGATCGGAATGTGTTTGACTGAAACTTTGGACGAAATGGTCCAGAACGGTACTCTTAGTCCTGAGCTTGCTATTCAGGTTCTTGTCCAGTTCGATAAGGTACAGCTAAAGCTGGTTTGGAGAGATGTGTTTATGTGTATATTGTGGATTTGTGTTCGGAATTAAGGTCTTGTTTGATTTAGTTTCCTACCGCTGTTGGCTTTTTGCAGTCCATGACCGAGGCTCTGGAATCACAGGTGAAAAGCAAGGTGTCTATCAAGGTATCTATGTTCGGCTTATGAATTTGACGAATCTCTGAATGTCAGAGATTTATGTTGCTAAATAAAGTCAACATTATAATCTTGAAACGTGTTTTTACTGATAGGAGGAATTGGTTGTGTTTTCCTGTTCTGAGATTGCTCTGTTTGTGCTCTAGTACACGTCTCGTCTTGAGCCAAATTGTTTTGTCAGTAAAACATACGTGGAATGTATAGTAGAATATTTTCACATGGTCTTGTGGTTGCCCAAATGGACAGCATAGGAACATTTGTATTTGAAAGTTGGAAGTTTCGTTTCTGGAAGTTTTATTGATACTAAAATAGATTAGGTTACCTGTGTTCATTCCGTTCCTCGTTTTTTCTCCATTGATTCTTGAAACATTTACCAGCCGTCTATAAAATATCATACCTTGAATATGTTTCTCTGGAATAATCTTCATAATTGATCTGTGTTTTTGGAATCTGGGGATGAAGTAATCTTGTATTAGGGATGTGGCTTTGCTGTAATATCTGGTTCTACAATGAGTCTTAATGATTCCCTTACTCTTGCTTTCAAGTTATATATTCAATCCGATGGCTGTTGGATTTGTTATGATACTTAGTTGAAAGTTCATTGTACATAGATTGTAGAACTATTTCTTTGAAAGAAATGATGAAATCACAATCACGCAGCAATATTTAACTGTTTTTTGATGTCGTCTTCCCAGCCATCACTTTTGACTCTTTCTTTCAGAAGACTAATCGATTTGTTTTTACTTTCGACATACTGCCAAGTGAATTAATTTTATAAGACTACTATGGTGATTTCTGAACCCCTTGGTCCTCCTACTGTTTTGCAACAGGGACATTTGCACACCTATCGGTTCTGCGACAATGTTTGGACATTCATTTTGCAGGATGCAACATTTAAGAACGAGGATTCCCAAGAGACTGTTGGAAGAATTAAAATAGTAGCATGTGACTCGAAGTTACTCTCACAATGA

mRNA sequence

ATGAGCTTTCTGTGCTCTTCGAGGGAATTGCTTTCGTACTTTGTTGGCGGGAAAGGTCGCCTAAGAAAATCGGTCAAATCTTTGACTTTATTACTCGATCTTGTTTTTCGAAGAGGAATGGCAACTTTTGAACTCTATCGCAGATCGACGATCGGAATGTGTTTGACTGAAACTTTGGACGAAATGGTCCAGAACGGTACTCTTAGTCCTGAGCTTGCTATTCAGGTTCTTGTCCAGTTCGATAAGTCCATGACCGAGGCTCTGGAATCACAGGTGAAAAGCAAGGTGTCTATCAAGTACACGTCTCGTCTTGAGCCAAATTGTTTTACTACTATGGTGATTTCTGAACCCCTTGGTCCTCCTACTGTTTTGCAACAGGGACATTTGCACACCTATCGGTTCTGCGACAATGTTTGGACATTCATTTTGCAGGATGCAACATTTAAGAACGAGGATTCCCAAGAGACTGTTGGAAGAATTAAAATAGTAGCATGTGACTCGAAGTTACTCTCACAATGA

Coding sequence (CDS)

ATGAGCTTTCTGTGCTCTTCGAGGGAATTGCTTTCGTACTTTGTTGGCGGGAAAGGTCGCCTAAGAAAATCGGTCAAATCTTTGACTTTATTACTCGATCTTGTTTTTCGAAGAGGAATGGCAACTTTTGAACTCTATCGCAGATCGACGATCGGAATGTGTTTGACTGAAACTTTGGACGAAATGGTCCAGAACGGTACTCTTAGTCCTGAGCTTGCTATTCAGGTTCTTGTCCAGTTCGATAAGTCCATGACCGAGGCTCTGGAATCACAGGTGAAAAGCAAGGTGTCTATCAAGTACACGTCTCGTCTTGAGCCAAATTGTTTTACTACTATGGTGATTTCTGAACCCCTTGGTCCTCCTACTGTTTTGCAACAGGGACATTTGCACACCTATCGGTTCTGCGACAATGTTTGGACATTCATTTTGCAGGATGCAACATTTAAGAACGAGGATTCCCAAGAGACTGTTGGAAGAATTAAAATAGTAGCATGTGACTCGAAGTTACTCTCACAATGA

Protein sequence

MSFLCSSRELLSYFVGGKGRLRKSVKSLTLLLDLVFRRGMATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKYTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACDSKLLSQ
Homology
BLAST of Spg016276 vs. NCBI nr
Match: KAG6608125.1 (Transcription initiation factor IIA subunit 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 203.8 bits (517), Expect = 1.2e-48
Identity = 109/140 (77.86%), Postives = 112/140 (80.00%), Query Frame = 0

Query: 33  DLVFRRGMATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQV 92
           +LVFRR MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQV
Sbjct: 610 NLVFRRRMATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQV 669

Query: 93  KSKVSIKYTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNED 152
           KSKV+IK                           GHLHTYRFCDNVWTFILQDATFKNED
Sbjct: 670 KSKVTIK---------------------------GHLHTYRFCDNVWTFILQDATFKNED 722

Query: 153 SQETVGRIKIVACDSKLLSQ 173
           SQETVGRIKIVACDSKLL+Q
Sbjct: 730 SQETVGRIKIVACDSKLLTQ 722

BLAST of Spg016276 vs. NCBI nr
Match: BBG95885.1 (transcription initiation factor IIA gamma chain / TFIIA-gamma [Prunus dulcis])

HSP 1 Score: 200.3 bits (508), Expect = 1.4e-47
Identity = 107/136 (78.68%), Postives = 114/136 (83.82%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALE+QVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALETQVKSKVSIK 60

Query: 100 Y--TSRLEPNCFTTMVISEPLGPPTVLQ-QGHLHTYRFCDNVWTFILQDATFKNEDSQET 159
           Y   S         + +S   G   VL+ +GHLHTYRFCDNVWTFILQDA FKNEDSQE 
Sbjct: 61  YWVISNWSRFWLRNLEVSGMKGLICVLENEGHLHTYRFCDNVWTFILQDALFKNEDSQEN 120

Query: 160 VGRIKIVACDSKLLSQ 173
           VGR+KIVACDSKLL+Q
Sbjct: 121 VGRVKIVACDSKLLTQ 136

BLAST of Spg016276 vs. NCBI nr
Match: KAF7817052.1 (transcription initiation factor IIA subunit 2 [Senna tora])

HSP 1 Score: 197.2 bits (500), Expect = 1.2e-46
Identity = 105/135 (77.78%), Postives = 112/135 (82.96%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPE+AIQVLVQFDKSMTEALE+ VKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPEIAIQVLVQFDKSMTEALETNVKSKVSIK 60

Query: 100 Y--TSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETV 159
           +   SR+ P C T              ++GHLHTYRFCDNVWTFILQDA FKNEDSQE V
Sbjct: 61  FDLDSRI-PICIT-------------FKKGHLHTYRFCDNVWTFILQDALFKNEDSQENV 120

Query: 160 GRIKIVACDSKLLSQ 173
           GR+KIVACDSKLLSQ
Sbjct: 121 GRVKIVACDSKLLSQ 121

BLAST of Spg016276 vs. NCBI nr
Match: KAG5052649.1 (hypothetical protein JHK87_004847 [Glycine soja])

HSP 1 Score: 196.4 bits (498), Expect = 2.0e-46
Identity = 106/142 (74.65%), Postives = 113/142 (79.58%), Query Frame = 0

Query: 33  DLVFRRGMATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQV 92
           D V +  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALE+QV
Sbjct: 13  DRVSQAAMATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALETQV 72

Query: 93  KSKVSIKYTSRLEPNCFTTMVISEPLGPPT--VLQQGHLHTYRFCDNVWTFILQDATFKN 152
           KSKVSIK             +  E LG  +  +   GHLHTYRFCDNVWTFILQDA FKN
Sbjct: 73  KSKVSIK----------VAFLFVEMLGWKSFDLFLLGHLHTYRFCDNVWTFILQDALFKN 132

Query: 153 EDSQETVGRIKIVACDSKLLSQ 173
           EDSQE VGR+KIVACDSKLL+Q
Sbjct: 133 EDSQENVGRVKIVACDSKLLTQ 144

BLAST of Spg016276 vs. NCBI nr
Match: XP_022936677.1 (transcription initiation factor IIA subunit 2 [Cucurbita moschata] >XP_022936678.1 transcription initiation factor IIA subunit 2 [Cucurbita moschata] >XP_022975864.1 transcription initiation factor IIA subunit 2 [Cucurbita maxima] >XP_023536591.1 transcription initiation factor IIA subunit 2 [Cucurbita pepo subsp. pepo] >XP_023536592.1 transcription initiation factor IIA subunit 2 [Cucurbita pepo subsp. pepo] >KAG7024584.1 Transcription initiation factor IIA subunit 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 196.1 bits (497), Expect = 2.6e-46
Identity = 105/133 (78.95%), Postives = 106/133 (79.70%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 106

Query: 160 IKIVACDSKLLSQ 173
           IKIVACDSKLL+Q
Sbjct: 121 IKIVACDSKLLTQ 106

BLAST of Spg016276 vs. ExPASy Swiss-Prot
Match: Q39236 (Transcription initiation factor IIA subunit 2 OS=Arabidopsis thaliana OX=3702 GN=TFIIA-S PE=3 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.1e-44
Identity = 96/133 (72.18%), Postives = 102/133 (76.69%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQ+GTLSPELAIQVLVQFDKSMTEALESQVK+KVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQSGTLSPELAIQVLVQFDKSMTEALESQVKTKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDA FK++D QE V R
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDAMFKSDDRQENVSR 106

Query: 160 IKIVACDSKLLSQ 173
           +KIVACDSKLL+Q
Sbjct: 121 VKIVACDSKLLTQ 106

BLAST of Spg016276 vs. ExPASy Swiss-Prot
Match: A2XZI2 (Transcription initiation factor IIA subunit 2 OS=Oryza sativa subsp. indica OX=39946 GN=TFIIAy PE=3 SV=2)

HSP 1 Score: 177.9 bits (450), Expect = 9.5e-44
Identity = 94/133 (70.68%), Postives = 102/133 (76.69%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMV +GTLSPELAIQVLVQFDKSMTEALE+QVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVSSGTLSPELAIQVLVQFDKSMTEALENQVKSKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFIL +A+FKNE++ E VG+
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILTEASFKNEETTEQVGK 106

Query: 160 IKIVACDSKLLSQ 173
           +KIVACDSKLLSQ
Sbjct: 121 VKIVACDSKLLSQ 106

BLAST of Spg016276 vs. ExPASy Swiss-Prot
Match: Q0DLD3 (Transcription initiation factor IIA subunit 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TFIIAy PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 9.5e-44
Identity = 94/133 (70.68%), Postives = 102/133 (76.69%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMV +GTLSPELAIQVLVQFDKSMTEALE+QVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVSSGTLSPELAIQVLVQFDKSMTEALENQVKSKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFIL +A+FKNE++ E VG+
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILTEASFKNEETTEQVGK 106

Query: 160 IKIVACDSKLLSQ 173
           +KIVACDSKLLSQ
Sbjct: 121 VKIVACDSKLLSQ 106

BLAST of Spg016276 vs. ExPASy Swiss-Prot
Match: O74948 (Transcription initiation factor IIA subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=toa2 PE=3 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.0e-21
Identity = 52/126 (41.27%), Postives = 79/126 (62.70%), Query Frame = 0

Query: 43  FELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKYTS 102
           +ELYRRS+IG+ LT+ LD+++  G +SP+LA++VL  FDKSMTEAL  +V+S+++ K   
Sbjct: 5   YELYRRSSIGISLTDALDDLISQGKISPQLAMKVLFNFDKSMTEALAEKVRSRLTFK--- 64

Query: 103 RLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKI 162
                                   GHL TYRFCD VWTFI+++ +F+ ++   T  +I+I
Sbjct: 65  ------------------------GHLDTYRFCDEVWTFIIKNPSFRFDNETVTSNKIRI 103

Query: 163 VACDSK 169
           VAC ++
Sbjct: 125 VACATR 103

BLAST of Spg016276 vs. ExPASy Swiss-Prot
Match: B7Q1Q9 (Transcription initiation factor IIA subunit 2 OS=Ixodes scapularis OX=6945 GN=IscW_ISCW010050 PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 5.1e-21
Identity = 50/127 (39.37%), Postives = 76/127 (59.84%), Query Frame = 0

Query: 42  TFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKYT 101
           +++LYR +T+G  L E+LDE++Q G ++P LA++VL+QFDK++  AL ++VK++++ K  
Sbjct: 2   SYQLYRNTTLGNTLQESLDELIQCGQITPHLALKVLLQFDKAINNALANRVKTRLTFK-- 61

Query: 102 SRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIK 161
                                    GHL TYRFCDNVWTF+L+D  F+         ++K
Sbjct: 62  ------------------------AGHLSTYRFCDNVWTFVLKDVEFREVQELVKADKVK 102

Query: 162 IVACDSK 169
           IVACD K
Sbjct: 122 IVACDGK 102

BLAST of Spg016276 vs. ExPASy TrEMBL
Match: A0A4Y1QVK2 (Transcription initiation factor IIA subunit 2 OS=Prunus dulcis OX=3755 GN=Prudu_004544 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 6.6e-48
Identity = 107/136 (78.68%), Postives = 114/136 (83.82%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALE+QVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALETQVKSKVSIK 60

Query: 100 Y--TSRLEPNCFTTMVISEPLGPPTVLQ-QGHLHTYRFCDNVWTFILQDATFKNEDSQET 159
           Y   S         + +S   G   VL+ +GHLHTYRFCDNVWTFILQDA FKNEDSQE 
Sbjct: 61  YWVISNWSRFWLRNLEVSGMKGLICVLENEGHLHTYRFCDNVWTFILQDALFKNEDSQEN 120

Query: 160 VGRIKIVACDSKLLSQ 173
           VGR+KIVACDSKLL+Q
Sbjct: 121 VGRVKIVACDSKLLTQ 136

BLAST of Spg016276 vs. ExPASy TrEMBL
Match: A0A6J1F935 (Transcription initiation factor IIA subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC111443199 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 1.2e-46
Identity = 105/133 (78.95%), Postives = 106/133 (79.70%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 106

Query: 160 IKIVACDSKLLSQ 173
           IKIVACDSKLL+Q
Sbjct: 121 IKIVACDSKLLTQ 106

BLAST of Spg016276 vs. ExPASy TrEMBL
Match: A0A6J1ILT9 (Transcription initiation factor IIA subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111476437 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 1.2e-46
Identity = 105/133 (78.95%), Postives = 106/133 (79.70%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 106

Query: 160 IKIVACDSKLLSQ 173
           IKIVACDSKLL+Q
Sbjct: 121 IKIVACDSKLLTQ 106

BLAST of Spg016276 vs. ExPASy TrEMBL
Match: A0A6J1BWA2 (Transcription initiation factor IIA subunit 2 OS=Momordica charantia OX=3673 GN=LOC111006284 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 2.1e-46
Identity = 105/133 (78.95%), Postives = 105/133 (78.95%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDATFKNEDSQE VGR
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDATFKNEDSQENVGR 106

Query: 160 IKIVACDSKLLSQ 173
           IKIVACDSKLLSQ
Sbjct: 121 IKIVACDSKLLSQ 106

BLAST of Spg016276 vs. ExPASy TrEMBL
Match: A0A6J1J100 (Transcription initiation factor IIA subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111480333 PE=3 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.8e-46
Identity = 104/133 (78.20%), Postives = 106/133 (79.70%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKV+IK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVTIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 106

Query: 160 IKIVACDSKLLSQ 173
           IKIVACDSKLL+Q
Sbjct: 121 IKIVACDSKLLTQ 106

BLAST of Spg016276 vs. TAIR 10
Match: AT4G24440.1 (transcription initiation factor IIA gamma chain / TFIIA-gamma (TFIIA-S) )

HSP 1 Score: 181.0 bits (458), Expect = 8.0e-46
Identity = 96/133 (72.18%), Postives = 102/133 (76.69%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQ+GTLSPELAIQVLVQFDKSMTEALESQVK+KVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQSGTLSPELAIQVLVQFDKSMTEALESQVKTKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDA FK++D QE V R
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDAMFKSDDRQENVSR 106

Query: 160 IKIVACDSKLLSQ 173
           +KIVACDSKLL+Q
Sbjct: 121 VKIVACDSKLLTQ 106

BLAST of Spg016276 vs. TAIR 10
Match: AT4G24440.2 (transcription initiation factor IIA gamma chain / TFIIA-gamma (TFIIA-S) )

HSP 1 Score: 181.0 bits (458), Expect = 8.0e-46
Identity = 96/133 (72.18%), Postives = 102/133 (76.69%), Query Frame = 0

Query: 40  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIK 99
           MATFELYRRSTIGMCLTETLDEMVQ+GTLSPELAIQVLVQFDKSMTEALESQVK+KVSIK
Sbjct: 1   MATFELYRRSTIGMCLTETLDEMVQSGTLSPELAIQVLVQFDKSMTEALESQVKTKVSIK 60

Query: 100 YTSRLEPNCFTTMVISEPLGPPTVLQQGHLHTYRFCDNVWTFILQDATFKNEDSQETVGR 159
                                      GHLHTYRFCDNVWTFILQDA FK++D QE V R
Sbjct: 61  ---------------------------GHLHTYRFCDNVWTFILQDAMFKSDDRQENVSR 106

Query: 160 IKIVACDSKLLSQ 173
           +KIVACDSKLL+Q
Sbjct: 121 VKIVACDSKLLTQ 106

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6608125.11.2e-4877.86Transcription initiation factor IIA subunit 2, partial [Cucurbita argyrosperma s... [more]
BBG95885.11.4e-4778.68transcription initiation factor IIA gamma chain / TFIIA-gamma [Prunus dulcis][more]
KAF7817052.11.2e-4677.78transcription initiation factor IIA subunit 2 [Senna tora][more]
KAG5052649.12.0e-4674.65hypothetical protein JHK87_004847 [Glycine soja][more]
XP_022936677.12.6e-4678.95transcription initiation factor IIA subunit 2 [Cucurbita moschata] >XP_022936678... [more]
Match NameE-valueIdentityDescription
Q392361.1e-4472.18Transcription initiation factor IIA subunit 2 OS=Arabidopsis thaliana OX=3702 GN... [more]
A2XZI29.5e-4470.68Transcription initiation factor IIA subunit 2 OS=Oryza sativa subsp. indica OX=3... [more]
Q0DLD39.5e-4470.68Transcription initiation factor IIA subunit 2 OS=Oryza sativa subsp. japonica OX... [more]
O749481.0e-2141.27Transcription initiation factor IIA subunit 2 OS=Schizosaccharomyces pombe (stra... [more]
B7Q1Q95.1e-2139.37Transcription initiation factor IIA subunit 2 OS=Ixodes scapularis OX=6945 GN=Is... [more]
Match NameE-valueIdentityDescription
A0A4Y1QVK26.6e-4878.68Transcription initiation factor IIA subunit 2 OS=Prunus dulcis OX=3755 GN=Prudu_... [more]
A0A6J1F9351.2e-4678.95Transcription initiation factor IIA subunit 2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1ILT91.2e-4678.95Transcription initiation factor IIA subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1BWA22.1e-4678.95Transcription initiation factor IIA subunit 2 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1J1002.8e-4678.20Transcription initiation factor IIA subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G24440.18.0e-4672.18transcription initiation factor IIA gamma chain / TFIIA-gamma (TFIIA-S) [more]
AT4G24440.28.0e-4672.18transcription initiation factor IIA gamma chain / TFIIA-gamma (TFIIA-S) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015872Transcription initiation factor IIA, gamma subunit, N-terminalPFAMPF02268TFIIA_gamma_Ncoord: 43..88
e-value: 3.1E-22
score: 78.4
IPR015872Transcription initiation factor IIA, gamma subunit, N-terminalCDDcd10145TFIIA_gamma_Ncoord: 43..88
e-value: 5.20213E-22
score: 81.8431
IPR015871Transcription initiation factor IIA, gamma subunit, C-terminalPFAMPF02751TFIIA_gamma_Ccoord: 127..167
e-value: 1.0E-20
score: 73.6
IPR015871Transcription initiation factor IIA, gamma subunit, C-terminalCDDcd10014TFIIA_gamma_Ccoord: 126..168
e-value: 1.87453E-21
score: 80.3695
IPR009088Transcription factor IIA, beta-barrelGENE3D2.30.18.10coord: 124..171
e-value: 4.5E-20
score: 73.2
IPR009088Transcription factor IIA, beta-barrelSUPERFAMILY50784Transcription factor IIA (TFIIA), beta-barrel domaincoord: 126..168
IPR009083Transcription factor IIA, alpha-helical domainGENE3D1.10.287.190coord: 42..93
e-value: 9.0E-24
score: 85.1
IPR003194Transcription initiation factor IIA, gamma subunitPANTHERPTHR10966TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2coord: 123..172
coord: 34..103
NoneNo IPR availablePANTHERPTHR10966:SF8TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2coord: 123..172
coord: 34..103
NoneNo IPR availableSUPERFAMILY47396Transcription factor IIA (TFIIA), alpha-helical domaincoord: 43..93

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016276.1Spg016276.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051123 RNA polymerase II preinitiation complex assembly
biological_process GO:0006413 translational initiation
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
cellular_component GO:0005672 transcription factor TFIIA complex
molecular_function GO:0016251 RNA polymerase II general transcription initiation factor activity
molecular_function GO:0017025 TBP-class protein binding
molecular_function GO:0003743 translation initiation factor activity