Spg016215 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016215 vs. NCBI nr
Match: XP_022980996.1 (protein ECERIFERUM 2 [Cucurbita maxima])

HSP 1 Score: 733.0 bits (1891), Expect = 1.5e-207
Identity = 374/439 (85.19%), Postives = 394/439 (89.75%), Query Frame = 0

Query: 1   MDGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  N LIS VK SSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNDLISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE Y VVSGRIRRR ED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 MILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINAL 180
            I HR G LV+SRA+GPDLGFSPLVFIQLT F+CGGL+VGLSWAHVLGDIFSASTFINA 
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDPLRP-PPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFR 240
           GH+MKNRPV+ LRP P  AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           I EEQL RI SV+ RNR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 IMEEQLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSAVEADFPVAGAEEGALAE+I+KKKIDE  EI ELVEKEREESDFIAYGAR
Sbjct: 301 QIPRNGMVMSAVEADFPVAGAEEGALAELIVKKKIDEDEEIKELVEKEREESDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYEIGGVGENG VLVLPGPPR DG  GGGRTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGTPGGGRTVTVIL 420

Query: 421 PEKLLPDLTDELQKQWAIV 439
           PEK LPDL DELQKQWAIV
Sbjct: 421 PEKQLPDLIDELQKQWAIV 439

BLAST of Spg016215 vs. NCBI nr
Match: XP_023523388.1 (protein ECERIFERUM 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 733.0 bits (1891), Expect = 1.5e-207
Identity = 376/439 (85.65%), Postives = 395/439 (89.98%), Query Frame = 0

Query: 1   MDGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  N LIS VKLSSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNDLISEVKLSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE Y VVSGRIRRR ED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 MILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINAL 180
            I HR G LV+SRA+GPDLGFSPLVFIQLT F+CGGL+VGLSWAHVLGDIFSAS FINA 
Sbjct: 121 KISHRHGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASNFINAW 180

Query: 181 GHIMKNRPVDPLRP-PPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFR 240
           GH+MKNRPV+ LRP P  AA LIRPS STGRST PVKRLDSTGDLWI S+D KMANRSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPVKRLDSTGDLWIASNDCKMANRSFR 240

Query: 241 ITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEEQL RI SV+ RNR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST  P+REG
Sbjct: 241 ITEEQLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKFPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSAVEADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKEREESDFIAYGAR
Sbjct: 301 QIPRNGMVMSAVEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREESDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYEIGGVGENG VLVLPGPPR DG  GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGTPGGDRTVTVIL 420

Query: 421 PEKLLPDLTDELQKQWAIV 439
           PEK LPDL DELQKQWAIV
Sbjct: 421 PEKQLPDLIDELQKQWAIV 439

BLAST of Spg016215 vs. NCBI nr
Match: KAG6608131.1 (Protein ECERIFERUM 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 731.1 bits (1886), Expect = 5.6e-207
Identity = 370/439 (84.28%), Postives = 396/439 (90.21%), Query Frame = 0

Query: 1   MDGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  N LIS VK SSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEE+RNLTI
Sbjct: 1   MDGSGNELISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEMRNLTI 60

Query: 61  YDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE Y VVSGRIRRR ED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 MILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINAL 180
            I HR G LV+SRA+GPDLGFSPLVFIQLT F+CGGL+VGLSWAHVLGDIFSASTFINA 
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDPLRP-PPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFR 240
           GH+MKNRPV+ LRP P  AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEE+L RI SV+ RNR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 ITEEKLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSA+EADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKERE+SDFIAYGAR
Sbjct: 301 QIPRNGMVMSAIEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREDSDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG E+EGQ+PVHVNYEIGGVGENG VLVLPGPPR DGR GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLEMEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGRPGGDRTVTVIL 420

Query: 421 PEKLLPDLTDELQKQWAIV 439
           PEK LP+L DELQKQWAIV
Sbjct: 421 PEKQLPELIDELQKQWAIV 439

BLAST of Spg016215 vs. NCBI nr
Match: XP_022940107.1 (protein ECERIFERUM 2 [Cucurbita moschata] >KAG7031769.1 Protein ECERIFERUM 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 729.9 bits (1883), Expect = 1.3e-206
Identity = 371/439 (84.51%), Postives = 395/439 (89.98%), Query Frame = 0

Query: 1   MDGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  N LIS VK SSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNELISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE Y VVSGRIRRR ED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 MILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINAL 180
            I HR G LV+SRA+GPDLGFSPLVFIQLT F+CGGL+VGLSWAHVLGDIFSASTFINA 
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDPLRP-PPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFR 240
           GH+MKNRPV+ LRP P  AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEE+L RI SV+ RNR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 ITEEKLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + P NGMVMSA+EADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKERE+SDFIAYGAR
Sbjct: 301 QIPINGMVMSAIEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREDSDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYEIGGVGENG VLVLPGPPR DGR GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGRPGGDRTVTVIL 420

Query: 421 PEKLLPDLTDELQKQWAIV 439
           PEK LP+L DELQKQWAIV
Sbjct: 421 PEKQLPELIDELQKQWAIV 439

BLAST of Spg016215 vs. NCBI nr
Match: XP_038898516.1 (protein ECERIFERUM 2 [Benincasa hispida])

HSP 1 Score: 706.8 bits (1823), Expect = 1.1e-199
Identity = 363/437 (83.07%), Postives = 380/437 (86.96%), Query Frame = 0

Query: 2   DGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIY 61
           DG +N+LIS +K SSVVPAKATG+NKV+ELTAIDLAMKLHYIRGVYFFR SEEVRNLTIY
Sbjct: 3   DGSKNTLISELKFSSVVPAKATGDNKVKELTAIDLAMKLHYIRGVYFFRASEEVRNLTIY 62

Query: 62  DLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDM 121
           DLKKP+F LLE Y VVSGRIRRR ED DRPFIKCNDSGVRIVEADCEKTI+EWLSI DD 
Sbjct: 63  DLKKPLFQLLELYYVVSGRIRRRIEDEDRPFIKCNDSGVRIVEADCEKTIEEWLSIGDDK 122

Query: 122 ILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINALG 181
           I HR   LVHS+A+GPDLGFSPL FIQLT FKCGGL+VGLSW HVLGDIFSASTFIN  G
Sbjct: 123 ISHRDDCLVHSQAIGPDLGFSPLAFIQLTRFKCGGLSVGLSWTHVLGDIFSASTFINVWG 182

Query: 182 HIMKNRPVDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFRIT 241
           +IM NRP D L P P A  L RPSRST  ST PVKRLD TGDLWIGSSD KMA RSFRIT
Sbjct: 183 YIMNNRPADHLLPAPAA--LTRPSRSTRLSTPPVKRLDPTGDLWIGSSDCKMATRSFRIT 242

Query: 242 EEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGES 301
            EQLDRI  V GRNRA NFS FEAIAAIFWK LSKIRLEDS+S+TISIYST  PNREGE 
Sbjct: 243 AEQLDRILGVFGRNRAVNFSTFEAIAAIFWKFLSKIRLEDSNSRTISIYSTKFPNREGEI 302

Query: 302 PRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLT 361
           PRNGM MS VEA+FPVAGA EG LAEMI+KKKIDEGGEI  LVEKER+ESDFIAYGARLT
Sbjct: 303 PRNGMEMSGVEAEFPVAGAAEGELAEMIVKKKIDEGGEIGGLVEKERDESDFIAYGARLT 362

Query: 362 FVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPE 421
           FVDLEEAN+YGFELEGQKPVHVNYEIGGVGENG VLVLPGPP GDGRDGGG TVTVILPE
Sbjct: 363 FVDLEEANIYGFELEGQKPVHVNYEIGGVGENGVVLVLPGPPCGDGRDGGGLTVTVILPE 422

Query: 422 KLLPDLTDELQKQWAIV 439
           K LPDL DELQKQW IV
Sbjct: 423 KELPDLIDELQKQWGIV 437

BLAST of Spg016215 vs. ExPASy Swiss-Prot
Match: Q39048 (Protein ECERIFERUM 2 OS=Arabidopsis thaliana OX=3702 GN=CER2 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.4e-83
Identity = 183/438 (41.78%), Postives = 269/438 (61.42%), Query Frame = 0

Query: 5   ENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLK 64
           E S ++ V+LSSVVPA   GENK ++LT +DLAMKLHY+R VYFF+ +   R+ T+ D+K
Sbjct: 2   EGSPVTSVRLSSVVPASVVGENKPRQLTPMDLAMKLHYVRAVYFFKGA---RDFTVADVK 61

Query: 65  KPMFL---LLEPYCVVSGRIRRRTEDGDR-----PFIKCNDSGVRIVEADCEK-TIDEWL 124
             MF    LL+ Y  VSGRIR    D D      P+I+CNDSG+R+VEA+ E+ T+++WL
Sbjct: 62  NTMFTLQSLLQSYHHVSGRIRMSDNDNDTSAAAIPYIRCNDSGIRVVEANVEEFTVEKWL 121

Query: 125 SIEDDMILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSAST 184
            ++D  I HR   LV+   LGPDL FSPLVF+Q+T FKCGGL +GLSWAH+LGD+FSAST
Sbjct: 122 ELDDRSIDHR--FLVYDHVLGPDLTFSPLVFLQITQFKCGGLCIGLSWAHILGDVFSAST 181

Query: 185 FINALGHIMK-NRPVDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMA 244
           F+  LG ++  + P  P+ P      L   +R+ G + + ++++DS G+ W+ ++  KM 
Sbjct: 182 FMKTLGQLVSGHAPTKPVYPK--TPELTSHARNDGEA-ISIEKIDSVGEYWLLTNKCKMG 241

Query: 245 NRSFRITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNC 304
              F  +   +D + +     R   FS  + + A+ WKSL  IR E +++  I+I    C
Sbjct: 242 RHIFNFSLNHIDSLMAKY-TTRDQPFSEVDILYALIWKSLLNIRGE-TNTNVITI----C 301

Query: 305 PNREGESPRN-GMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDF 364
             ++  +  N  +V+S VE +  + G  E  LA +I  +K +E G I  ++E+++  SDF
Sbjct: 302 DRKKSSTCWNEDLVISVVEKNDEMVGISE--LAALIAGEKREENGAIKRMIEQDKGSSDF 361

Query: 365 IAYGARLTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGR 424
             YGA LTFV+L+E ++Y  E+ G KP  VNY I GVG+ G VLV P       +    R
Sbjct: 362 FTYGANLTFVNLDEIDMYELEINGGKPDFVNYTIHGVGDKGVVLVFP-------KQNFAR 416

Query: 425 TVTVILPEKLLPDLTDEL 432
            V+V++PE+ L  L +E+
Sbjct: 422 IVSVVMPEEDLAKLKEEV 416

BLAST of Spg016215 vs. ExPASy Swiss-Prot
Match: Q9LIS1 (Protein ECERIFERUM 26-like OS=Arabidopsis thaliana OX=3702 GN=CER26L PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 7.7e-66
Identity = 154/425 (36.24%), Postives = 233/425 (54.82%), Query Frame = 0

Query: 9   ISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMF 68
           + G +LS+V  +  +      E T +DLAMKLHY++ VY +  +   R+LT+ D+K P+F
Sbjct: 12  VHGFRLSTVSASLPSETGTTHEPTGLDLAMKLHYLKAVYIY-SAGTARDLTVMDVKAPLF 71

Query: 69  LLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDMILHRGGG 128
            +      + GR RR   +  RP++KCND G R VE+ C+ T++EWL + D  +      
Sbjct: 72  SVFYQIPCIIGRFRR--HESGRPYLKCNDCGTRFVESHCDLTVEEWLRVPDRSV---DES 131

Query: 129 LVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMKNRP 188
           LV+ + +GPDL FSPL++IQ+T F CGGLA+GLSWAH++GD FS S F N          
Sbjct: 132 LVYHQPVGPDLAFSPLLYIQMTRFSCGGLALGLSWAHIMGDPFSLSHFFNLWAQAFAGGK 191

Query: 189 VDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFRITEEQLDRI 248
           +   +          P+ ST +    VK++D  GDLW+  ++ KM   SF +T   L   
Sbjct: 192 IYCPKTSVTERDFQNPT-STFKKPDSVKQVDLVGDLWVAPNNSKMTTFSFNLTVNDLKTH 251

Query: 249 FSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRNGMVM 308
           F V G         FE +  I WK ++ +R E S   TI++  ++    +  + RNG ++
Sbjct: 252 FPVNGD------GEFEILTGIIWKCVATVRGE-SAPVTITVIRSDPKKLKPRAVRNGQMI 311

Query: 309 SAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVDLEEA 368
           S++  DF VA A    + + I + K DE   I E+V+   + SDFI YGA LTFVD+ E 
Sbjct: 312 SSIHVDFSVAEASLEEIVKSIGEAK-DERVVIDEIVD---DVSDFIVYGANLTFVDMSEV 371

Query: 369 NLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKLLPDLT 428
           + Y  ++ G+ P  V   + G+G++GAV+VLPG    +      R VTV LP   +  + 
Sbjct: 372 DFYEAKVMGKSPESVYCNVQGIGDDGAVVVLPGVVEEE------RVVTVTLPVDEIEKVK 412

Query: 429 DELQK 434
            E++K
Sbjct: 432 WEMKK 412

BLAST of Spg016215 vs. ExPASy Swiss-Prot
Match: Q9SVM9 (Protein ECERIFERUM 26 OS=Arabidopsis thaliana OX=3702 GN=CER26 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.2e-64
Identity = 149/426 (34.98%), Postives = 230/426 (53.99%), Query Frame = 0

Query: 9   ISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMF 68
           +  ++LS+V   + T      E T +DLAMKLHY++  Y +  +E  R+LT+  LK+ MF
Sbjct: 14  VHSIRLSTVGATRPTETGTTHEPTGLDLAMKLHYLKAAYIY-SAETARDLTVRHLKEAMF 73

Query: 69  LLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDMILHRGGG 128
           +L +     +GR  RR  D  RP+IKCND G R VE  C  T++EWLS  D  +      
Sbjct: 74  MLFDQIAWTTGRFSRR--DSGRPYIKCNDCGTRFVEGQCNLTVEEWLSKPDRSV---DEF 133

Query: 129 LVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMKNRP 188
           LV+   +GP+L FSPL+++Q+T FKCGGL +GLSWA+++GD FS     N     +    
Sbjct: 134 LVYHHPIGPELTFSPLIYVQMTRFKCGGLGLGLSWANIIGDAFSLFYAFNLWAKAITGEK 193

Query: 189 VDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFRIT-EEQLDR 248
           +     P +     +    T +  + +KR++  GDLW+  +D K+AN  F ++  +Q+  
Sbjct: 194 IYAPTTPSIGERRFQSPNPTVKDPVSIKRVEPVGDLWVTPNDKKLANYCFNLSVADQISP 253

Query: 249 IFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRNGMV 308
            F   G +   +   FE +A I WK ++K+R+E     T++I   +  + +  + RN  V
Sbjct: 254 HFPAKGDD---SIPVFEILAGIIWKCIAKVRVEPK-PVTVTIIKKDPNDLKLNAIRNSQV 313

Query: 309 MSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVDLEE 368
           +S+V  DFPVA A    L + + + K DE   I E+ E      DF+ YGA+LTF+DL  
Sbjct: 314 ISSVSVDFPVAEATVEELVKAMGEAK-DERCGIEEIGESCDGNLDFVVYGAKLTFLDLTG 373

Query: 369 ANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKLLPDL 428
            +LY  ++ G+ P  V   + G+GE G V+V       +      R VTV LPE+ +  +
Sbjct: 374 EDLYEAKVMGKSPESVYCNVEGIGEEGLVVVYAAAKSEE------RVVTVTLPEEEMERV 422

Query: 429 TDELQK 434
             E +K
Sbjct: 434 KLEFKK 422

BLAST of Spg016215 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ1 (Hydroxycinnamoyltransferase OS=Narcissus pseudonarcissus OX=39639 GN=HCT PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 6.0e-18
Identity = 91/332 (27.41%), Postives = 150/332 (45.18%), Query Frame = 0

Query: 8   LISGVKLSSVV-PAKATGENKVQELTAIDLAMKLHYIRGVYFF-RPSEEVRNLTIYDLKK 67
           +I  +K +++V P++ T   ++   + +DL +   +   VYF+ RP           LK+
Sbjct: 1   MIINIKETTMVRPSQPTPSQRLWN-SNLDLVVPRFHTPSVYFYRRPGNASDFFDARVLKE 60

Query: 68  PMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDMILHR 127
            +   L P+  ++GR+  R EDG R  I CN  GVR V A+ +  IDE+      M L +
Sbjct: 61  ALGRALVPFYPMAGRL-ARDEDG-RVEIDCNGEGVRFVVAETDSAIDEFGDFAPTMELKK 120

Query: 128 GGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMK 187
              L+     G D+   PL+ +Q+T FKCGG ++G+   H + D  S   FIN+   I  
Sbjct: 121 ---LIPKVEYGDDISAFPLLVLQITHFKCGGTSLGVGMQHHVADGASGLHFINSWSDIA- 180

Query: 188 NRPVDPLRPPPVAAPLIR---------------PSRSTGRSTLPVKRLDSTGDLWIGSSD 247
            R +D   PP +   L+R               P+ S   S  P++      D  + S  
Sbjct: 181 -RGLDIAVPPFIDRSLLRARDPPSPSFPHIEYQPAPSMNTSPAPIQ------DPTVKSDP 240

Query: 248 FKMANRSFRITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIY 307
              A   F++T++QLD + S +    +A +S++  +A   W+  S  R    D +T    
Sbjct: 241 TATAVSIFKLTKQQLDLLKSRV----SAKYSSYALVAGHVWRCTSIARGLPDDQRTKLYC 300

Query: 308 STNCPNREGESPRNGMVMSAVEADFPVAGAEE 323
           +T+   R      +G   + +    PVA A E
Sbjct: 301 ATDGRARLQPPLPSGYFGNVIFTATPVADAGE 314

BLAST of Spg016215 vs. ExPASy Swiss-Prot
Match: Q9FI78 (Shikimate O-hydroxycinnamoyltransferase OS=Arabidopsis thaliana OX=3702 GN=HST PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.8e-14
Identity = 71/276 (25.72%), Postives = 124/276 (44.93%), Query Frame = 0

Query: 34  IDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFI 93
           +DL +   +   VYF+RP+          +K+ +   L P+  ++GR++R  +D  R  I
Sbjct: 27  VDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSKALVPFYPMAGRLKR--DDDGRIEI 86

Query: 94  KCNDSGVRIVEADCEKTIDEWLSIEDDMILHRGGGLVHSRALGPDLGFS------PLVFI 153
            CN +GV  V AD    ID++      + L         R L P++  S      PL+ +
Sbjct: 87  DCNGAGVLFVVADTPSVIDDFGDFAPTLNL---------RQLIPEVDHSAGIHSFPLLVL 146

Query: 154 QLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMKNRPVDPLRPPPVAAPLIRPSRS 213
           Q+T+FKCGG ++G+   H   D FS   FIN    +   R +D   PP +   L+R +R 
Sbjct: 147 QVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMA--RGLDLTIPPFIDRTLLR-ARD 206

Query: 214 TGRSTLPVKRLDSTGDLWIGSSDFKMANRS-----FRITEEQLDRIFSVIGRN-RAANFS 273
             +             + I     K    +     F++T +QL  + +    +    ++S
Sbjct: 207 PPQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVALKAKSKEDGNTVSYS 266

Query: 274 NFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNR 298
           ++E +A   W+S+ K R   +D +T    +T+  +R
Sbjct: 267 SYEMLAGHVWRSVGKARGLPNDQETKLYIATDGRSR 288

BLAST of Spg016215 vs. ExPASy TrEMBL
Match: A0A6J1IV89 (protein ECERIFERUM 2 OS=Cucurbita maxima OX=3661 GN=LOC111480277 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 7.2e-208
Identity = 374/439 (85.19%), Postives = 394/439 (89.75%), Query Frame = 0

Query: 1   MDGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  N LIS VK SSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNDLISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE Y VVSGRIRRR ED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 MILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINAL 180
            I HR G LV+SRA+GPDLGFSPLVFIQLT F+CGGL+VGLSWAHVLGDIFSASTFINA 
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDPLRP-PPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFR 240
           GH+MKNRPV+ LRP P  AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           I EEQL RI SV+ RNR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 IMEEQLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + PRNGMVMSAVEADFPVAGAEEGALAE+I+KKKIDE  EI ELVEKEREESDFIAYGAR
Sbjct: 301 QIPRNGMVMSAVEADFPVAGAEEGALAELIVKKKIDEDEEIKELVEKEREESDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYEIGGVGENG VLVLPGPPR DG  GGGRTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGTPGGGRTVTVIL 420

Query: 421 PEKLLPDLTDELQKQWAIV 439
           PEK LPDL DELQKQWAIV
Sbjct: 421 PEKQLPDLIDELQKQWAIV 439

BLAST of Spg016215 vs. ExPASy TrEMBL
Match: A0A6J1FIP6 (protein ECERIFERUM 2 OS=Cucurbita moschata OX=3662 GN=LOC111445827 PE=3 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 6.1e-207
Identity = 371/439 (84.51%), Postives = 395/439 (89.98%), Query Frame = 0

Query: 1   MDGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  N LIS VK SSVVPAKATGENKV EL+AIDLAMKLHYIRGVYFFRPSEEVRNLTI
Sbjct: 1   MDGSGNELISEVKFSSVVPAKATGENKVHELSAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60

Query: 61  YDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDD 120
           YDLKKPMF LLE Y VVSGRIRRR ED DRPFIKCNDSGVRIVE +CEKTIDEWLSI+DD
Sbjct: 61  YDLKKPMFPLLEIYYVVSGRIRRRIEDRDRPFIKCNDSGVRIVEVECEKTIDEWLSIDDD 120

Query: 121 MILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINAL 180
            I HR G LV+SRA+GPDLGFSPLVFIQLT F+CGGL+VGLSWAHVLGDIFSASTFINA 
Sbjct: 121 KISHRDGDLVYSRAIGPDLGFSPLVFIQLTRFRCGGLSVGLSWAHVLGDIFSASTFINAW 180

Query: 181 GHIMKNRPVDPLRP-PPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFR 240
           GH+MKNRPV+ LRP P  AA LIRPS STGRST P+KRLDSTGDLWI S+D KMA RSFR
Sbjct: 181 GHVMKNRPVERLRPLPATAADLIRPSGSTGRSTSPIKRLDSTGDLWIASNDCKMAKRSFR 240

Query: 241 ITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREG 300
           ITEE+L RI SV+ RNR+AN+S+FEAIAAIFWK LSKIRLEDSDSKTISIYST CP+REG
Sbjct: 241 ITEEKLVRILSVVDRNRSANYSSFEAIAAIFWKCLSKIRLEDSDSKTISIYSTKCPDREG 300

Query: 301 ESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGAR 360
           + P NGMVMSA+EADFPVAGAEEGALAE+I+KKKIDEG EI ELVEKERE+SDFIAYGAR
Sbjct: 301 QIPINGMVMSAIEADFPVAGAEEGALAELIVKKKIDEGEEIKELVEKEREDSDFIAYGAR 360

Query: 361 LTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVIL 420
           LTFVDLEEANLYG ELEGQ+PVHVNYEIGGVGENG VLVLPGPPR DGR GG RTVTVIL
Sbjct: 361 LTFVDLEEANLYGLELEGQRPVHVNYEIGGVGENGVVLVLPGPPRCDGRPGGDRTVTVIL 420

Query: 421 PEKLLPDLTDELQKQWAIV 439
           PEK LP+L DELQKQWAIV
Sbjct: 421 PEKQLPELIDELQKQWAIV 439

BLAST of Spg016215 vs. ExPASy TrEMBL
Match: A0A6J1C6H6 (protein ECERIFERUM 2 OS=Momordica charantia OX=3673 GN=LOC111008780 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 3.4e-189
Identity = 348/440 (79.09%), Postives = 374/440 (85.00%), Query Frame = 0

Query: 1   MDGGENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTI 60
           MDG  +SLISGVK SSVVPAKA+GENKVQELTA+DL MKLHYIRGVYFFRPSEE+RNLTI
Sbjct: 1   MDGASDSLISGVKFSSVVPAKASGENKVQELTAMDLVMKLHYIRGVYFFRPSEEIRNLTI 60

Query: 61  YDLKKPMFLLLEPYCVVSGRIRRRTE-DGDRPFIKCNDSGVRIVEADCEKTIDEWLSIED 120
           YDLKKPMF LLE Y VVSGRIRRR E  G+RPFIKCNDSGVRIVEADC+K+IDEWLS+ED
Sbjct: 61  YDLKKPMFPLLELYFVVSGRIRRRIEAGGNRPFIKCNDSGVRIVEADCQKSIDEWLSMED 120

Query: 121 DMILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINA 180
           +   HR  GLVHSRALGPDLGFSPLVFIQLT FKCGGLAVGLSWAHVLGDIFSAS FIN 
Sbjct: 121 EKYSHRDHGLVHSRALGPDLGFSPLVFIQLTRFKCGGLAVGLSWAHVLGDIFSASAFINV 180

Query: 181 LGHIMKN-RPVDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSF 240
            GHIM N RPV  LR       + RPS ST ++  PVKRLD TGDLWIGSSD KMA RSF
Sbjct: 181 WGHIMNNRRPVCQLRTH--TPTICRPSGSTTQTRSPVKRLDQTGDLWIGSSDCKMATRSF 240

Query: 241 RITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNRE 300
           RI EEQL+RI S++ RNRA  FSNFEA+AAIFWKSLSKIR  DSD KTISIYST CPNRE
Sbjct: 241 RIMEEQLERILSIVRRNRAVAFSNFEALAAIFWKSLSKIR-PDSDLKTISIYSTKCPNRE 300

Query: 301 GESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGA 360
           GE P NGMVMSAV+AD PV GAEEGALAE+I++K+IDEGGEI ELVEKE EESDFIAYG 
Sbjct: 301 GEIPMNGMVMSAVKADAPVTGAEEGALAELILEKRIDEGGEIEELVEKEMEESDFIAYGG 360

Query: 361 RLTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVI 420
           RLTFVDLEEAN+YG EL GQKPVH NYEIGGVG++G VLVLPGPPR  G  GGGR VTVI
Sbjct: 361 RLTFVDLEEANIYGLELGGQKPVHANYEIGGVGDDGVVLVLPGPPRDGGGYGGGRNVTVI 420

Query: 421 LPEKLLPDLTDELQKQWAIV 439
           +PEK LP L DELQKQWAI+
Sbjct: 421 IPEKQLPALIDELQKQWAII 437

BLAST of Spg016215 vs. ExPASy TrEMBL
Match: A0A1S3CQJ9 (protein ECERIFERUM 1 OS=Cucumis melo OX=3656 GN=LOC103503654 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.4e-187
Identity = 347/446 (77.80%), Postives = 379/446 (84.98%), Query Frame = 0

Query: 1   MDG-GENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLT 60
           MDG  + + IS +K SSVVPAKATGE++V+ELTA+DLAMKLHYIRGVYFFR SEEV+NLT
Sbjct: 1   MDGVAKTTPISDLKFSSVVPAKATGEDEVKELTAMDLAMKLHYIRGVYFFRASEEVKNLT 60

Query: 61  IYDLKKPMFLLLEPYCVVSGRIRRR---TEDGDRPFIKCNDSGVRIVEADCEKTIDEWLS 120
           IYDLKKP+FLLLE Y VVSGRIRRR     DGDR FIKCNDSGVRIVEADCEKTI+EWLS
Sbjct: 61  IYDLKKPLFLLLEKYYVVSGRIRRRIIGDNDGDRAFIKCNDSGVRIVEADCEKTIEEWLS 120

Query: 121 IE---DDMILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSA 180
           IE   DD IL+R G LVHS+A+GPDLGFSPL FIQLT FKCGGL+VGLSW HVLGDIFSA
Sbjct: 121 IEDDDDDKILNRDGCLVHSQAIGPDLGFSPLAFIQLTRFKCGGLSVGLSWTHVLGDIFSA 180

Query: 181 STFINALGHIMKNRPVDPLRPPPVAAPLIRPSRSTGR-STLPVKRLDSTGDLWIGSSDFK 240
           STFIN  GHIM NRP+  LRP P  A  I PS S+   ST P+KRLD TGDLWIGS+D K
Sbjct: 181 STFINVWGHIMNNRPLHQLRPAP--ATHIPPSTSSRLISTPPLKRLDPTGDLWIGSTDCK 240

Query: 241 MANRSFRITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYST 300
           MA RSFRIT EQLDRI SV+GRNR+ NFS FE+I A+FWKSLSKIRLEDS S+TISIYST
Sbjct: 241 MATRSFRITSEQLDRITSVVGRNRSVNFSTFESITAVFWKSLSKIRLEDSGSRTISIYST 300

Query: 301 NCPNREGESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESD 360
             PNREGE P NGM MS VEADFPVAGA EG LAE+I+KKKIDEGGEI ELVE +  +SD
Sbjct: 301 KFPNREGEIPSNGMEMSGVEADFPVAGAAEGELAEVIVKKKIDEGGEIEELVETKMNDSD 360

Query: 361 FIAYGARLTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGG 420
           FIAYGARLTFVDLEEA++YGFELEGQK VHVNYEIGGVGENG VLVLPGPPR DGRDGGG
Sbjct: 361 FIAYGARLTFVDLEEADIYGFELEGQKAVHVNYEIGGVGENGVVLVLPGPPRSDGRDGGG 420

Query: 421 RTVTVILPEKLLPDLTDELQKQWAIV 439
           RTVTVILPEK LP+L DE++KQW IV
Sbjct: 421 RTVTVILPEKELPELIDEMEKQWGIV 444

BLAST of Spg016215 vs. ExPASy TrEMBL
Match: A0A0A0LEQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G892170 PE=3 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 3.1e-187
Identity = 340/443 (76.75%), Postives = 378/443 (85.33%), Query Frame = 0

Query: 1   MDG-GENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLT 60
           MDG  + + +S ++ SSVVPAKATGE++V+ELTA+DLAMKLHYIRGVYFFR SEEVRNLT
Sbjct: 1   MDGVTKTTPVSDLRFSSVVPAKATGEDEVKELTAMDLAMKLHYIRGVYFFRASEEVRNLT 60

Query: 61  IYDLKKPMFLLLEPYCVVSGRIRRRT--EDGDRPFIKCNDSGVRIVEADCEKTIDEWLSI 120
           +YDLKKP+FLLLE Y VVSGRIRRR   +DGDR FIKCNDSGVRIVEADCEKTI+EWLSI
Sbjct: 61  VYDLKKPLFLLLEKYYVVSGRIRRRIVGDDGDRAFIKCNDSGVRIVEADCEKTIEEWLSI 120

Query: 121 ED-DMILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTF 180
           ED D IL+R G LVHS+A+GPDLGFSPL FIQLT FKCGGL+VGLSW HVLGDIFSASTF
Sbjct: 121 EDGDKILNRDGCLVHSQAIGPDLGFSPLAFIQLTRFKCGGLSVGLSWTHVLGDIFSASTF 180

Query: 181 INALGHIMKNRPVDPLRPPPVA-APLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMAN 240
           IN  GHIM NRP+  LRP P    P  R SR    ST P+KRLD TGDLWIGS+D KMA 
Sbjct: 181 INVWGHIMNNRPLHQLRPAPATHIPTSRSSRLI--STPPLKRLDPTGDLWIGSTDCKMAT 240

Query: 241 RSFRITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCP 300
           RSFRIT  QLDRI SV+GRNR+ NFS FE+I A+FW SLSKIRLEDSDS+TISIYST CP
Sbjct: 241 RSFRITSAQLDRITSVVGRNRSMNFSTFESITAVFWNSLSKIRLEDSDSRTISIYSTKCP 300

Query: 301 NREGESPRNGMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIA 360
           NRE E P NGM MS VEADFPVAGA EG LAE+I+KK+IDEGGE+ ELVE + ++SDFIA
Sbjct: 301 NRESEIPSNGMEMSGVEADFPVAGAAEGELAEVIVKKRIDEGGEVEELVETKMDDSDFIA 360

Query: 361 YGARLTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTV 420
           YGARLTFVDLEEA++YGFELEGQK VHVNYEIGGVGENG V+VLPGPPR DGRDGGGRTV
Sbjct: 361 YGARLTFVDLEEADVYGFELEGQKAVHVNYEIGGVGENGVVMVLPGPPRSDGRDGGGRTV 420

Query: 421 TVILPEKLLPDLTDELQKQWAIV 439
           TVILPEK +PDL DE++KQW IV
Sbjct: 421 TVILPEKEVPDLIDEMEKQWGIV 441

BLAST of Spg016215 vs. TAIR 10
Match: AT4G24510.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.7e-84
Identity = 183/438 (41.78%), Postives = 269/438 (61.42%), Query Frame = 0

Query: 5   ENSLISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLK 64
           E S ++ V+LSSVVPA   GENK ++LT +DLAMKLHY+R VYFF+ +   R+ T+ D+K
Sbjct: 2   EGSPVTSVRLSSVVPASVVGENKPRQLTPMDLAMKLHYVRAVYFFKGA---RDFTVADVK 61

Query: 65  KPMFL---LLEPYCVVSGRIRRRTEDGDR-----PFIKCNDSGVRIVEADCEK-TIDEWL 124
             MF    LL+ Y  VSGRIR    D D      P+I+CNDSG+R+VEA+ E+ T+++WL
Sbjct: 62  NTMFTLQSLLQSYHHVSGRIRMSDNDNDTSAAAIPYIRCNDSGIRVVEANVEEFTVEKWL 121

Query: 125 SIEDDMILHRGGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSAST 184
            ++D  I HR   LV+   LGPDL FSPLVF+Q+T FKCGGL +GLSWAH+LGD+FSAST
Sbjct: 122 ELDDRSIDHR--FLVYDHVLGPDLTFSPLVFLQITQFKCGGLCIGLSWAHILGDVFSAST 181

Query: 185 FINALGHIMK-NRPVDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMA 244
           F+  LG ++  + P  P+ P      L   +R+ G + + ++++DS G+ W+ ++  KM 
Sbjct: 182 FMKTLGQLVSGHAPTKPVYPK--TPELTSHARNDGEA-ISIEKIDSVGEYWLLTNKCKMG 241

Query: 245 NRSFRITEEQLDRIFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNC 304
              F  +   +D + +     R   FS  + + A+ WKSL  IR E +++  I+I    C
Sbjct: 242 RHIFNFSLNHIDSLMAKY-TTRDQPFSEVDILYALIWKSLLNIRGE-TNTNVITI----C 301

Query: 305 PNREGESPRN-GMVMSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDF 364
             ++  +  N  +V+S VE +  + G  E  LA +I  +K +E G I  ++E+++  SDF
Sbjct: 302 DRKKSSTCWNEDLVISVVEKNDEMVGISE--LAALIAGEKREENGAIKRMIEQDKGSSDF 361

Query: 365 IAYGARLTFVDLEEANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGR 424
             YGA LTFV+L+E ++Y  E+ G KP  VNY I GVG+ G VLV P       +    R
Sbjct: 362 FTYGANLTFVNLDEIDMYELEINGGKPDFVNYTIHGVGDKGVVLVFP-------KQNFAR 416

Query: 425 TVTVILPEKLLPDLTDEL 432
            V+V++PE+ L  L +E+
Sbjct: 422 IVSVVMPEEDLAKLKEEV 416

BLAST of Spg016215 vs. TAIR 10
Match: AT3G23840.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 252.7 bits (644), Expect = 5.5e-67
Identity = 154/425 (36.24%), Postives = 233/425 (54.82%), Query Frame = 0

Query: 9   ISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMF 68
           + G +LS+V  +  +      E T +DLAMKLHY++ VY +  +   R+LT+ D+K P+F
Sbjct: 12  VHGFRLSTVSASLPSETGTTHEPTGLDLAMKLHYLKAVYIY-SAGTARDLTVMDVKAPLF 71

Query: 69  LLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDMILHRGGG 128
            +      + GR RR   +  RP++KCND G R VE+ C+ T++EWL + D  +      
Sbjct: 72  SVFYQIPCIIGRFRR--HESGRPYLKCNDCGTRFVESHCDLTVEEWLRVPDRSV---DES 131

Query: 129 LVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMKNRP 188
           LV+ + +GPDL FSPL++IQ+T F CGGLA+GLSWAH++GD FS S F N          
Sbjct: 132 LVYHQPVGPDLAFSPLLYIQMTRFSCGGLALGLSWAHIMGDPFSLSHFFNLWAQAFAGGK 191

Query: 189 VDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFRITEEQLDRI 248
           +   +          P+ ST +    VK++D  GDLW+  ++ KM   SF +T   L   
Sbjct: 192 IYCPKTSVTERDFQNPT-STFKKPDSVKQVDLVGDLWVAPNNSKMTTFSFNLTVNDLKTH 251

Query: 249 FSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRNGMVM 308
           F V G         FE +  I WK ++ +R E S   TI++  ++    +  + RNG ++
Sbjct: 252 FPVNGD------GEFEILTGIIWKCVATVRGE-SAPVTITVIRSDPKKLKPRAVRNGQMI 311

Query: 309 SAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVDLEEA 368
           S++  DF VA A    + + I + K DE   I E+V+   + SDFI YGA LTFVD+ E 
Sbjct: 312 SSIHVDFSVAEASLEEIVKSIGEAK-DERVVIDEIVD---DVSDFIVYGANLTFVDMSEV 371

Query: 369 NLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKLLPDLT 428
           + Y  ++ G+ P  V   + G+G++GAV+VLPG    +      R VTV LP   +  + 
Sbjct: 372 DFYEAKVMGKSPESVYCNVQGIGDDGAVVVLPGVVEEE------RVVTVTLPVDEIEKVK 412

Query: 429 DELQK 434
            E++K
Sbjct: 432 WEMKK 412

BLAST of Spg016215 vs. TAIR 10
Match: AT4G13840.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 247.3 bits (630), Expect = 2.3e-65
Identity = 149/426 (34.98%), Postives = 230/426 (53.99%), Query Frame = 0

Query: 9   ISGVKLSSVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMF 68
           +  ++LS+V   + T      E T +DLAMKLHY++  Y +  +E  R+LT+  LK+ MF
Sbjct: 14  VHSIRLSTVGATRPTETGTTHEPTGLDLAMKLHYLKAAYIY-SAETARDLTVRHLKEAMF 73

Query: 69  LLLEPYCVVSGRIRRRTEDGDRPFIKCNDSGVRIVEADCEKTIDEWLSIEDDMILHRGGG 128
           +L +     +GR  RR  D  RP+IKCND G R VE  C  T++EWLS  D  +      
Sbjct: 74  MLFDQIAWTTGRFSRR--DSGRPYIKCNDCGTRFVEGQCNLTVEEWLSKPDRSV---DEF 133

Query: 129 LVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMKNRP 188
           LV+   +GP+L FSPL+++Q+T FKCGGL +GLSWA+++GD FS     N     +    
Sbjct: 134 LVYHHPIGPELTFSPLIYVQMTRFKCGGLGLGLSWANIIGDAFSLFYAFNLWAKAITGEK 193

Query: 189 VDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFRIT-EEQLDR 248
           +     P +     +    T +  + +KR++  GDLW+  +D K+AN  F ++  +Q+  
Sbjct: 194 IYAPTTPSIGERRFQSPNPTVKDPVSIKRVEPVGDLWVTPNDKKLANYCFNLSVADQISP 253

Query: 249 IFSVIGRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNREGESPRNGMV 308
            F   G +   +   FE +A I WK ++K+R+E     T++I   +  + +  + RN  V
Sbjct: 254 HFPAKGDD---SIPVFEILAGIIWKCIAKVRVEPK-PVTVTIIKKDPNDLKLNAIRNSQV 313

Query: 309 MSAVEADFPVAGAEEGALAEMIMKKKIDEGGEIVELVEKEREESDFIAYGARLTFVDLEE 368
           +S+V  DFPVA A    L + + + K DE   I E+ E      DF+ YGA+LTF+DL  
Sbjct: 314 ISSVSVDFPVAEATVEELVKAMGEAK-DERCGIEEIGESCDGNLDFVVYGAKLTFLDLTG 373

Query: 369 ANLYGFELEGQKPVHVNYEIGGVGENGAVLVLPGPPRGDGRDGGGRTVTVILPEKLLPDL 428
            +LY  ++ G+ P  V   + G+GE G V+V       +      R VTV LPE+ +  +
Sbjct: 374 EDLYEAKVMGKSPESVYCNVEGIGEEGLVVVYAAAKSEE------RVVTVTLPEEEMERV 422

Query: 429 TDELQK 434
             E +K
Sbjct: 434 KLEFKK 422

BLAST of Spg016215 vs. TAIR 10
Match: AT4G29250.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 93.2 bits (230), Expect = 5.6e-19
Identity = 116/452 (25.66%), Postives = 205/452 (45.35%), Query Frame = 0

Query: 16  SVVPAKATGENKVQELTAIDLAMKLHYIRGVYFFRPSE--EVRNLTIYDLKKPMFLLLEP 75
           +VV  ++    ++ +L+ +D  M+ ++IR VY++R S+  E   +T   L++ +   L  
Sbjct: 21  TVVSTRSIEPGRLYQLSVLDHVMEPNHIRLVYYYRCSKTREPGEIT-KKLRESLAYTLNC 80

Query: 76  YCVVSGRIRRRTED-------GDRPFIKCNDSGVRIVEADCEKTIDEWL-SIEDDMILHR 135
           Y +V+GR+ +  +          R  +K ND+GVR+VEA    ++ EWL S+  +  L  
Sbjct: 81  YPIVTGRLVKEVDGMEENKDLSQRWMVKSNDAGVRMVEARATGSVKEWLRSVNREEELK- 140

Query: 136 GGGLVHSRALGPDLGFSPLVFIQLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMK 195
              LVH   +     +     +Q+T F+ GGLA+GLS +H+L D   A  FI A   +  
Sbjct: 141 ---LVHWEDMYHLQYYWSTFCVQVTEFESGGLAIGLSCSHLLADPVCAMMFIRAWADLTL 200

Query: 196 NRPVDPLRPPPVAAPLIRPSRSTGRSTLPVKRLDSTGDLWIGSSDFKMANRSFRITEEQL 255
           +R    +  PP+  PL  P R + +  +   +L S    +I S     +  +  +TE+ +
Sbjct: 201 SR---SMMAPPLFHPL-PPRRFSNQRLISNNQLLSH---YIKSCSLTASPSN--MTEDHM 260

Query: 256 DRIFSVI-------GRNRAANFSNFEAIAAIFWKSLSKIRLEDSDSKTISI--------- 315
             +  +        G N     S FE +A +FW  +S+ + + ++   +S+         
Sbjct: 261 VTVTFLFPDPLVRAGENE-PRISTFEILAGLFWVCVSRAKGKRNELMDMSLCLDVRKLLR 320

Query: 316 ----YSTNCPNREGESPRNGMVMSAVEADFPVAGAEE---GALAEMIMKKKIDEGGEIVE 375
               Y  NC           MV   V    PV   ++    A+ E+    K  +   +++
Sbjct: 321 LDQSYFGNC-----------MVYHKVPYSKPVKTKDKLLFHAVQEIESITKRLDYDTVMD 380

Query: 376 LVEKEREESDFIAYGARLTFVDLE---EANLYGFELEGQKPVHVN-YEIGGVGENGAVLV 431
           L+E     +  I+ G+ L   +LE    +    FE E     HV+ Y  G V   G V+V
Sbjct: 381 LIEWLSSNNGAISNGSDLVCTNLENMSHSRPMMFE-EDLALSHVSCYVEGPVAGGGQVIV 440

BLAST of Spg016215 vs. TAIR 10
Match: AT5G48930.1 (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase )

HSP 1 Score: 82.0 bits (201), Expect = 1.3e-15
Identity = 71/276 (25.72%), Postives = 124/276 (44.93%), Query Frame = 0

Query: 34  IDLAMKLHYIRGVYFFRPSEEVRNLTIYDLKKPMFLLLEPYCVVSGRIRRRTEDGDRPFI 93
           +DL +   +   VYF+RP+          +K+ +   L P+  ++GR++R  +D  R  I
Sbjct: 27  VDLVIPRFHTPSVYFYRPTGASNFFDPQVMKEALSKALVPFYPMAGRLKR--DDDGRIEI 86

Query: 94  KCNDSGVRIVEADCEKTIDEWLSIEDDMILHRGGGLVHSRALGPDLGFS------PLVFI 153
            CN +GV  V AD    ID++      + L         R L P++  S      PL+ +
Sbjct: 87  DCNGAGVLFVVADTPSVIDDFGDFAPTLNL---------RQLIPEVDHSAGIHSFPLLVL 146

Query: 154 QLTWFKCGGLAVGLSWAHVLGDIFSASTFINALGHIMKNRPVDPLRPPPVAAPLIRPSRS 213
           Q+T+FKCGG ++G+   H   D FS   FIN    +   R +D   PP +   L+R +R 
Sbjct: 147 QVTFFKCGGASLGVGMQHHAADGFSGLHFINTWSDMA--RGLDLTIPPFIDRTLLR-ARD 206

Query: 214 TGRSTLPVKRLDSTGDLWIGSSDFKMANRS-----FRITEEQLDRIFSVIGRN-RAANFS 273
             +             + I     K    +     F++T +QL  + +    +    ++S
Sbjct: 207 PPQPAFHHVEYQPAPSMKIPLDPSKSGPENTTVSIFKLTRDQLVALKAKSKEDGNTVSYS 266

Query: 274 NFEAIAAIFWKSLSKIRLEDSDSKTISIYSTNCPNR 298
           ++E +A   W+S+ K R   +D +T    +T+  +R
Sbjct: 267 SYEMLAGHVWRSVGKARGLPNDQETKLYIATDGRSR 288