Spg016196 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016196
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	E3 SUMO-protein ligase MMS21
Location	scaffold9: 38069685 .. 38072705 (+)
RNA-Seq Expression	Spg016196
Synteny	Spg016196

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGGGATTCTCCAAAATTCGAACCAGTTTGTTTGACGAAGCGGGTATTCTCTTTTGGCGCTTAAACAAACCATCGCCGGAGGAGTTTCCGTTTCGACAATCTCTTCTTCAACCGAATGGCTTCCGCTTCCAATTCCCGTTCAAGTGGCGTTTCCGATAGAATCAAATCCGCTGCCACTATTATGCACTCCGAGAATCAATCCCTTCTTGCCGTAACTTCCCTTCATACTACCCTCTAGTCTACTTCTATGACTGCTTTTAATTTCTCTTCGTTTTCGTTCTAGTTCTTGCGTTTTTCTGATTCATAGAGGGATTTTTGTTCTGGGAAATCCGGCTCTGGAAAGAAAAATGAAGTCGCTGTTGCTAAATCAGTGTCGGTTTCAGCTTTAAGATCTCGATTTTTTTGTATAGCGAGTCAGTCCTTATGCTGTATTCCCTTCACTCTCCGCTGTGAACTGGGTTTTCTTGATTTCTGAAAGCTAAAACGTCAGGGGCTTGAGTGATTTGTTAGGAATTTGATTGATGATGATGGGTTATCTGTTTGTTTTTGTGATTTGATTTTACTGTCTTAATTTCAGGAATTACGGAAGTCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAGAAGGACAATCAGTCGAGGATGGTAATTTTATTGATAATGAACCTTAATTTTGAGCATACAAAATGATGGTGGTTTGTTACTTTGGAAGATAATGTTTTCAAAACAAGGTACTTTTGTAAAATGTTAAGAAGCAAGGACAACACAAATTTCAAAGATTTTCTCTTGGACTCTCATCATAAGGCTTTATTTTAACATCTTTGGAGTTTCTTTCTTAGTTAATTGAAAATAAAATGTGAGTGAAGGAAAACAAGATATATGTGCAGCGTATTGTGTTGTGTACAACCTGCTTTGGAACTTTCACTATAAACCAGCCACTTTGTCAAACCGTTCAAAATTCAAATGTTTGAAATTCTTAATTCTATTCAGTGATTTTTCTTGCTTAGGTTTTTGGTTTTTGATAGACTTTCTATGGATGCTTACGATATGATCGAGATTTATTTGAATTGATTCCTTGCTTAAAAATTATGATCTTGGTTTCACTTGGTGTACATTAACTAATTACTATACCAAATATACTTTTTGGTGGATTTTCTGCTTCTGTCTATGAGATCCAATCTAAAGATGATTGAGAATCATTGTTGTTTGGAACTCAACCAATAATTTACTTGGTATTTGAACATTTTCTTAGGTCAAGGAGCTTGAAAATTCTGTTGTTGAGCTGCTGGGTACTTATGAATCCTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAACACATATGAACCAAAAGAAGAGGTGCTTAAATCTCTTTCCATGTTGAACTTTCCACTACACATTCTTTTATTAATTTTCTTTGTGCTGGTTCCTTCTTTTTCACACCTAATATTTAGGTTGATATTGTAGTTAACAGATTTTGAGAAACTACTCGACGACGAAGTTGCAAAAATCAGCGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAATTTTAGAGAAGCTATCTGGGTATGCATATTCATCTTCTTTTATACGCATTAGGAAGTGAAAGTATTCCAGTTTTATTATCTGATTCTAGGAAACTTTGGAATGCGTTGCCTCCCTTCTTTGACTTTCTATTTGTCGGGCTTTTGCTTTGATTATACATGGCAGCAACAAGAGAATCCTCAAATCTGTGCAAATATTTATCCTGAAACTGTGAAAATGACCAACAGAAAGTACATGAGTAGTAAACAAGAGAAACTAGGTTACCTGTTTGCAATTTACAAATGTTTGGAAAATATTTTATTGTTCTTACAATTGTAGATTTTATTCTCTCCAGTGCTTTAGCCTTTAGCCTATCTTCCGATATTCCTGCAGAATGTTCATCATGCAGGACAGCCGATGCCAGGTGAGGAACAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCATTGAGTGGAAAGCCTGTCACTGAATTAGCGGAACCGGTTCGCAGGTACACTAAAGATATTTTGATGAAGGATAAAGAATTGGAATTATTTCTCTTACTGTTGAAAAAGAAGTTTGCTCATCTATTCGCTCTGTAATAACAGCATGAATGTTTAAAGGGAATGGCTACATGCTTTATTTTCTCTAGTTACATTTTAGCCTGAAAATGGAATGAGATATTCTGTCACTGGGATGAGTTTAGAAATTCTTCTTGTGTAGGTCCTCTTGTTTTTCCATGGTTTTTTGTTTTTTCGAGTTCAACCACGTGTAGGGATGGAGATTCAAAATTCTGACATTTTAGTCGAGTATATAATATCTTAACTAGTTTAGTATGCTCATATTAGTGCAATGGCTCAGCCATCATACATTTAACACTTTTATTAAGTCTCTTTTTGTTTCTACCTTCTGAAATTTGTAGTATGGAATGCAAGCACGTATACGAAAAGGCGGTCATAATGCAGTACCTAAAATCGAAGAAATCTCGAGCTCAATGCCCTGTTGCTGGTATTCAGATCTTCTGCATTACATGTACGACATACAATATGTTATTGTATTAGATTGCTCGTCTGACTTAATGTCTTTGTCCTTGTAGCCTGTCCTAGGATGTTGCAGCCCGAAAAGGTCATTTCTGATCCATTCTTACAGATTGAAATTGATGAACTGCGAAAAGTGTCTAGGCATTCTGTGAGGATACAGGATTTCACAGAGCTTGATGCAGATTAGCAGCACTGAAGTAGTTTCTTTTCCTCTTTTTGTTATTGCTATTATTATTATTGTCGTCGTTGTCGTTGTCGTCGCTTTCTGAATCAAATGTTTTGTTGGTAAGTTGAGCTAGTTGGGATGTACATGGTATCTATAGTTGGTCTATATTCATGTGCACATTGAGTTGAATGGAGAAATGTGGCTTCATTTACAAATTGTCATTGCCCTTTATATTTTGAGCTGTTCTTT

mRNA sequence

ATGGCTTCCGCTTCCAATTCCCGTTCAAGTGGCGTTTCCGATAGAATCAAATCCGCTGCCACTATTATGCACTCCGAGAATCAATCCCTTCTTGCCAGGGATTTTTGTTCTGGGAAATCCGGCTCTGGAAAGAAAAATGAAGTCGCTGAATTACGGAAGTCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAGAAGGACAATCAGTCGAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTTGAGCTGCTGGGTACTTATGAATCCTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAACACATATGAACCAAAAGAAGAGTTAACAGATTTTGAGAAACTACTCGACGACGAAGTTGCAAAAATCAGCGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAATTTTAGAGAAGCTATCTGGAATGTTCATCATGCAGGACAGCCGATGCCAGGTGAGGAACAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCATTGAGTGGAAAGCCTGTCACTGAATTAGCGGAACCGGTTCGCAGTATGGAATGCAAGCACGTATACGAAAAGGCGGTCATAATGCAGTACCTAAAATCGAAGAAATCTCGAGCTCAATGCCCTGTTGCTGCCTGTCCTAGGATGTTGCAGCCCGAAAAGGTCATTTCTGATCCATTCTTACAGATTGAAATTGATGAACTGCGAAAAGTGTCTAGGCATTCTGTGAGGATACAGGATTTCACAGAGCTTGATGCAGATTAG

Coding sequence (CDS)

ATGGCTTCCGCTTCCAATTCCCGTTCAAGTGGCGTTTCCGATAGAATCAAATCCGCTGCCACTATTATGCACTCCGAGAATCAATCCCTTCTTGCCAGGGATTTTTGTTCTGGGAAATCCGGCTCTGGAAAGAAAAATGAAGTCGCTGAATTACGGAAGTCGCTGATTATGATGAAGGAAATTGGGGTGGATTTGGAGAAGGACAATCAGTCGAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTTGAGCTGCTGGGTACTTATGAATCCTGTAACAACTTTTCATCTGCAATTCAGTCGGTTGGAAACACATATGAACCAAAAGAAGAGTTAACAGATTTTGAGAAACTACTCGACGACGAAGTTGCAAAAATCAGCGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAATTTTAGAGAAGCTATCTGGAATGTTCATCATGCAGGACAGCCGATGCCAGGTGAGGAACAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCATTGAGTGGAAAGCCTGTCACTGAATTAGCGGAACCGGTTCGCAGTATGGAATGCAAGCACGTATACGAAAAGGCGGTCATAATGCAGTACCTAAAATCGAAGAAATCTCGAGCTCAATGCCCTGTTGCTGCCTGTCCTAGGATGTTGCAGCCCGAAAAGGTCATTTCTGATCCATTCTTACAGATTGAAATTGATGAACTGCGAAAAGTGTCTAGGCATTCTGTGAGGATACAGGATTTCACAGAGCTTGATGCAGATTAG

Protein sequence

MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKEIGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEIDELRKVSRHSVRIQDFTELDAD

Homology

[Close]

BLAST of Spg016196 vs. NCBI nr
Match: XP_022941288.1 (E3 SUMO-protein ligase MMS21 [Cucurbita moschata])

HSP 1 Score: 432.2 bits (1110), Expect = 3.3e-117
Identity = 225/265 (84.91%), Postives = 238/265 (89.81%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MAS SNS S+GVS+RIKSAATIM+SENQSLL                 AELRKSLIMMKE
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLL-----------------AELRKSLIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IGV+LE+DNQSRMVKELENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLDD
Sbjct: 61  IGVELERDNQSRMVKELENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDD 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+SENSSSNLQNHSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSENSSSNLQNHSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPVTELAEPVRS ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL 
Sbjct: 181 SGKPVTELAEPVRSTECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLP 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK S+HSVRIQDFTE+DAD
Sbjct: 241 IEIDELRKTSKHSVRIQDFTEVDAD 248

[Close]

BLAST of Spg016196 vs. NCBI nr
Match: XP_023525588.1 (E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-protein ligase MMS21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 431.8 bits (1109), Expect = 4.3e-117
Identity = 225/265 (84.91%), Postives = 238/265 (89.81%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MAS SNS S+GVS+RIKSAATIM+SENQSLL                 AELRKSLIMMKE
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLL-----------------AELRKSLIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IGVDLE+DNQSRMVKELENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+
Sbjct: 61  IGVDLERDNQSRMVKELENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDN 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+SENSSSNLQNHSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSENSSSNLQNHSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPVTELAEPVRS ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL 
Sbjct: 181 SGKPVTELAEPVRSTECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLP 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK S+HSVRIQDFTE+DAD
Sbjct: 241 IEIDELRKTSKHSVRIQDFTEVDAD 248

[Close]

BLAST of Spg016196 vs. NCBI nr
Match: KAG6607898.1 (E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429.9 bits (1104), Expect = 1.6e-116
Identity = 224/265 (84.53%), Postives = 237/265 (89.43%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MAS SNS S+GVS+RIKSAATIM+SENQSLL                 AELRKSLIMMKE
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLL-----------------AELRKSLIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IGVDLE+DNQSRMVKELENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+
Sbjct: 61  IGVDLERDNQSRMVKELENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDN 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+SENSSSNL NHSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSENSSSNLHNHSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPVTELAEPVRS ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL 
Sbjct: 181 SGKPVTELAEPVRSTECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLP 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK S+HSVRIQDFTE+DAD
Sbjct: 241 IEIDELRKTSKHSVRIQDFTEVDAD 248

[Close]

BLAST of Spg016196 vs. NCBI nr
Match: XP_022981951.1 (E3 SUMO-protein ligase MMS21 [Cucurbita maxima])

HSP 1 Score: 425.6 bits (1093), Expect = 3.1e-115
Identity = 222/263 (84.41%), Postives = 235/263 (89.35%), Query Frame = 0


Query: 3   SASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKEIG 62
           S SNS S+GVS+RIKSAATIM+SENQSLL                 AELRKSLIMMKEIG
Sbjct: 5   SNSNSNSTGVSERIKSAATIMNSENQSLL-----------------AELRKSLIMMKEIG 64

Query: 63  VDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEV 122
           VDLE+DNQSRMVKELENSVVELL TYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EV
Sbjct: 65  VDLERDNQSRMVKELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEV 124

Query: 123 AKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSG 182
           AK+SENSSSNLQNHSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSG
Sbjct: 125 AKVSENSSSNLQNHSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSG 184

Query: 183 KPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIE 242
           KPVTELAEPVRS ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IE
Sbjct: 185 KPVTELAEPVRSTECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIE 244

Query: 243 IDELRKVSRHSVRIQDFTELDAD 266
           IDELRK S+HSVRIQDFTE+DAD
Sbjct: 245 IDELRKTSKHSVRIQDFTEVDAD 250

[Close]

BLAST of Spg016196 vs. NCBI nr
Match: XP_038897059.1 (E3 SUMO-protein ligase MMS21 isoform X1 [Benincasa hispida])

HSP 1 Score: 419.5 bits (1077), Expect = 2.2e-113
Identity = 219/265 (82.64%), Postives = 234/265 (88.30%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MASAS+SRS+GVS RIKSAATIMHSENQSLL                 AELRK+LIMMKE
Sbjct: 1   MASASDSRSNGVSGRIKSAATIMHSENQSLL-----------------AELRKALIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IGV+LE+DNQSRMVKELENSVVELL TYE+CNNFS AIQSVGN YEPKEELTDF KLLDD
Sbjct: 61  IGVELERDNQSRMVKELENSVVELLSTYENCNNFSLAIQSVGNIYEPKEELTDFGKLLDD 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+S++SSSNL NHSIIR FREA+WNVHHAGQPMPGEE+EDIVMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSQDSSSNLMNHSIIRQFREAVWNVHHAGQPMPGEEKEDIVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPV EL EPVRS ECKHVYEKA IMQYLKSKKSRAQCPVAACP+MLQ +KV+ DPFLQ
Sbjct: 181 SGKPVIELTEPVRSAECKHVYEKAAIMQYLKSKKSRAQCPVAACPKMLQSDKVVPDPFLQ 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK+SRHS RIQDFTELDAD
Sbjct: 241 IEIDELRKISRHSDRIQDFTELDAD 248

[Close]

BLAST of Spg016196 vs. ExPASy Swiss-Prot
Match: Q8GYH7 (E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.4e-70
Identity = 143/261 (54.79%), Postives = 186/261 (71.26%), Query Frame = 0


Query: 4   ASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKEIGV 63
           AS S S GV+ RI++A+ ++ S+N S L                 A++RK++ MMK I V
Sbjct: 2   ASASSSDGVAGRIQNASLVLVSDNSSTL-----------------ADIRKAVAMMKNIAV 61

Query: 64  DLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVA 123
            LEK+NQ+  VK+LENSV ELL  +  CN+ S+AIQSV N Y+P E+LTDF+KLLDDE  
Sbjct: 62  QLEKENQTDKVKDLENSVAELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFT 121

Query: 124 KISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGK 183
           K+    SS  QN  ++R FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGK
Sbjct: 122 KLKATPSSVPQNDHLMRQFREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGK 181

Query: 184 PVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEI 243
           PVTELA+PVRSM+C+HVYEK+VI+ Y+ +  + A CPVA C   LQ  KVI D  L+ EI
Sbjct: 182 PVTELADPVRSMDCRHVYEKSVILHYIVNNPN-ANCPVAGCRGKLQNSKVICDAMLKFEI 241

Query: 244 DELRKVSRHSVR---IQDFTE 262
           +E+R +++ S R   I+DFTE
Sbjct: 242 EEMRSLNKQSNRAEVIEDFTE 244

[Close]

BLAST of Spg016196 vs. ExPASy TrEMBL
Match: A0A6J1FRP6 (E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.6e-117
Identity = 225/265 (84.91%), Postives = 238/265 (89.81%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MAS SNS S+GVS+RIKSAATIM+SENQSLL                 AELRKSLIMMKE
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLL-----------------AELRKSLIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IGV+LE+DNQSRMVKELENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLDD
Sbjct: 61  IGVELERDNQSRMVKELENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDD 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+SENSSSNLQNHSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSENSSSNLQNHSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPVTELAEPVRS ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL 
Sbjct: 181 SGKPVTELAEPVRSTECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLP 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK S+HSVRIQDFTE+DAD
Sbjct: 241 IEIDELRKTSKHSVRIQDFTEVDAD 248

[Close]

BLAST of Spg016196 vs. ExPASy TrEMBL
Match: A0A6J1IXZ2 (E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.5e-115
Identity = 222/263 (84.41%), Postives = 235/263 (89.35%), Query Frame = 0


Query: 3   SASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKEIG 62
           S SNS S+GVS+RIKSAATIM+SENQSLL                 AELRKSLIMMKEIG
Sbjct: 5   SNSNSNSTGVSERIKSAATIMNSENQSLL-----------------AELRKSLIMMKEIG 64

Query: 63  VDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEV 122
           VDLE+DNQSRMVKELENSVVELL TYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EV
Sbjct: 65  VDLERDNQSRMVKELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEV 124

Query: 123 AKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSG 182
           AK+SENSSSNLQNHSIIR FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSG
Sbjct: 125 AKVSENSSSNLQNHSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSG 184

Query: 183 KPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIE 242
           KPVTELAEPVRS ECKHVYEK  IMQYLKSK+SRAQCPVAACP+MLQPEKVISDPFL IE
Sbjct: 185 KPVTELAEPVRSTECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIE 244

Query: 243 IDELRKVSRHSVRIQDFTELDAD 266
           IDELRK S+HSVRIQDFTE+DAD
Sbjct: 245 IDELRKTSKHSVRIQDFTEVDAD 250

[Close]

BLAST of Spg016196 vs. ExPASy TrEMBL
Match: A0A5D3DWX9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001530 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.9e-113
Identity = 216/265 (81.51%), Postives = 233/265 (87.92%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MASAS+SRS+GV+ RIKSAATIMHSENQSLL                 AELRK+LIMMKE
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLL-----------------AELRKTLIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IGVDLEK+NQ++MVKELE S+VELL  YE+CNNFSSAIQSVGNTYEPKEELTDFEKLLDD
Sbjct: 61  IGVDLEKENQNKMVKELEKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+S NSSSN  NH IIR FREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSVNSSSNFANHPIIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPVTELAEPVRS ECKH+YEKA IMQYL SKKSRAQCPVAACP+MLQP+KV+ DPFL+
Sbjct: 181 SGKPVTELAEPVRSAECKHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLE 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK+SRHS RIQDFTELDAD
Sbjct: 241 IEIDELRKMSRHSERIQDFTELDAD 248

[Close]

BLAST of Spg016196 vs. ExPASy TrEMBL
Match: A0A1S3CLF9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.9e-113
Identity = 216/265 (81.51%), Postives = 233/265 (87.92%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MASAS+SRS+GV+ RIKSAATIMHSENQSLL                 AELRK+LIMMKE
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLL-----------------AELRKTLIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IGVDLEK+NQ++MVKELE S+VELL  YE+CNNFSSAIQSVGNTYEPKEELTDFEKLLDD
Sbjct: 61  IGVDLEKENQNKMVKELEKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+S NSSSN  NH IIR FREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSVNSSSNFANHPIIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPVTELAEPVRS ECKH+YEKA IMQYL SKKSRAQCPVAACP+MLQP+KV+ DPFL+
Sbjct: 181 SGKPVTELAEPVRSAECKHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLE 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK+SRHS RIQDFTELDAD
Sbjct: 241 IEIDELRKMSRHSERIQDFTELDAD 248

[Close]

BLAST of Spg016196 vs. ExPASy TrEMBL
Match: A0A6J1CCS6 (E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 9.0e-113
Identity = 216/265 (81.51%), Postives = 233/265 (87.92%), Query Frame = 0


Query: 1   MASASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKE 60
           MAS S++RSS VS RIKSAAT+MHSENQSLL                 AE+RK LIMMKE
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLL-----------------AEIRKLLIMMKE 60

Query: 61  IGVDLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDD 120
           IG+DLE+DNQS MVK+LEN+VVELL TYE+C+NFSSAIQSVGN YEP+EELTDFEKLLDD
Sbjct: 61  IGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDD 120

Query: 121 EVAKISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180
           EVAK+SENSSSNLQNHSIIR FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL
Sbjct: 121 EVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPL 180

Query: 181 SGKPVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQ 240
           SGKPVTELA+PVRSMECKHVYEK  +MQYL+SK SRAQCPVAACP+MLQPEKV  DPFL 
Sbjct: 181 SGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLL 240

Query: 241 IEIDELRKVSRHSVRIQDFTELDAD 266
           IEIDELRK SRHSVRIQDFTELDA+
Sbjct: 241 IEIDELRKTSRHSVRIQDFTELDAE 248

[Close]

BLAST of Spg016196 vs. TAIR 10
Match: AT3G15150.1 (RING/U-box superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.7e-71
Identity = 143/261 (54.79%), Postives = 186/261 (71.26%), Query Frame = 0


Query: 4   ASNSRSSGVSDRIKSAATIMHSENQSLLARDFCSGKSGSGKKNEVAELRKSLIMMKEIGV 63
           AS S S GV+ RI++A+ ++ S+N S L                 A++RK++ MMK I V
Sbjct: 2   ASASSSDGVAGRIQNASLVLVSDNSSTL-----------------ADIRKAVAMMKNIAV 61

Query: 64  DLEKDNQSRMVKELENSVVELLGTYESCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVA 123
            LEK+NQ+  VK+LENSV ELL  +  CN+ S+AIQSV N Y+P E+LTDF+KLLDDE  
Sbjct: 62  QLEKENQTDKVKDLENSVAELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFT 121

Query: 124 KISENSSSNLQNHSIIRNFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGK 183
           K+    SS  QN  ++R FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGK
Sbjct: 122 KLKATPSSVPQNDHLMRQFREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGK 181

Query: 184 PVTELAEPVRSMECKHVYEKAVIMQYLKSKKSRAQCPVAACPRMLQPEKVISDPFLQIEI 243
           PVTELA+PVRSM+C+HVYEK+VI+ Y+ +  + A CPVA C   LQ  KVI D  L+ EI
Sbjct: 182 PVTELADPVRSMDCRHVYEKSVILHYIVNNPN-ANCPVAGCRGKLQNSKVICDAMLKFEI 241

Query: 244 DELRKVSRHSVR---IQDFTE 262
           +E+R +++ S R   I+DFTE
Sbjct: 242 EEMRSLNKQSNRAEVIEDFTE 244

The following BLAST results are available for this feature:

BLAST of Spg016196 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941288.1	3.3e-117	84.91	E3 SUMO-protein ligase MMS21 [Cucurbita moschata]	[more]
XP_023525588.1	4.3e-117	84.91	E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-...	[more]
KAG6607898.1	1.6e-116	84.53	E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022981951.1	3.1e-115	84.41	E3 SUMO-protein ligase MMS21 [Cucurbita maxima]	[more]
XP_038897059.1	2.2e-113	82.64	E3 SUMO-protein ligase MMS21 isoform X1 [Benincasa hispida]	[more]

BLAST of Spg016196 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYH7	2.4e-70	54.79	E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1	[more]

BLAST of Spg016196 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRP6	1.6e-117	84.91	E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 ...	[more]
A0A6J1IXZ2	1.5e-115	84.41	E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV...	[more]
A0A5D3DWX9	6.9e-113	81.51	E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CLF9	6.9e-113	81.51	E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1	[more]
A0A6J1CCS6	9.0e-113	81.51	E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of Spg016196 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15150.1	1.7e-71	54.79	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 171..255 e-value: 9.6E-18 score: 66.2
IPR004181	Zinc finger, MIZ-type	PFAM	PF11789	zf-Nse	coord: 162..224 e-value: 3.2E-14 score: 52.6
IPR004181	Zinc finger, MIZ-type	CDD	cd16651	SPL-RING_NSE2	coord: 177..245 e-value: 7.84653E-23 score: 86.9148
IPR026846	E3 SUMO-protein ligase Nse2 (Mms21)	PANTHER	PTHR21330	UNCHARACTERIZED	coord: 6..252
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 176..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016196.1	Spg016196.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0060250	germ-line stem-cell niche homeostasis
biological_process	GO:0032876	negative regulation of DNA endoreduplication
biological_process	GO:0008284	positive regulation of cell population proliferation
biological_process	GO:0080038	positive regulation of cytokinin-activated signaling pathway
biological_process	GO:0045931	positive regulation of mitotic cell cycle
biological_process	GO:0016925	protein sumoylation
biological_process	GO:0048509	regulation of meristem development
biological_process	GO:0010082	regulation of root meristem growth
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030915	Smc5-Smc6 complex
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0061665	SUMO ligase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0019789	SUMO transferase activity