Spg016126 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016126 vs. NCBI nr
Match: KAE8800683.1 (retrotransposon unclassified [Hordeum vulgare])

HSP 1 Score: 212.2 bits (539), Expect = 2.3e-50
Identity = 249/1061 (23.47%), Postives = 428/1061 (40.34%), Query Frame = 0

Query: 92   RILTPKLINPEVFKTFMPRIWNKEGRVRIKAMGRNTYLCNFNNSFEKDRIKRGGPWNYDR 151
            +IL+ KL +P+     + ++W     +  K MG N ++  F     K R    GPW +D 
Sbjct: 35   KILSEKLAHPDAISLSLGKVWCPIKGINCKEMGENRFVFTFMQDSGKRRAIEDGPWMFDN 94

Query: 152  ALLVIEDTQGASRISHTSFRYVNFWVHIHDLPMVCMRRKWAEKLGNSLGEFVEADLDEGG 211
             L+V+E+     R+    F  +  WV +++LP+  M  + AE +GN +G+FVEAD    G
Sbjct: 95   DLVVVEEFDAQKRLEEYEFNNIPIWVRVYNLPLGMMNEESAEDIGNIIGQFVEADTGVDG 154

Query: 212  STENT-LRIQVKIDVSEPLKRGLMV------------RIGSKAEET-WVKVTYERLPEFC 271
            S     LRI++++ + +PL RG  +             +G + + + W +  YE LP+FC
Sbjct: 155  SAIGMYLRIKIRMRIDKPLMRGFTLDDDDERKKHKGKNMGKEEDGSGWCRFEYEFLPDFC 214

Query: 272  YGCGIIGHVQQECEKISNEGEENLYGDFM-------RATPIVGGTPKQKTQENKRGNFWG 331
            Y CG++GH +++C     +GE+  +G ++       RA    GG  K            G
Sbjct: 215  YTCGLLGHGEKDCNTKLQKGEKQQFGRWIKADMGHRRAAGDAGGWRK-----------GG 274

Query: 332  RERGRRGAYQFQNNRQNQKYNDEKEETWRRRDQSGKSNEENVGVLRPENSTGGR--SSPE 391
            RE G +  Y +  +R N +     +    R+D    S +       PEN+  G   +SP 
Sbjct: 275  RENGTQRNYGY--SRSNGRTGSGSDSLSWRKDGFRASGD------GPENTEKGEEVTSPV 334

Query: 392  KETVGKSQ-GQPTASEVSEQSRMKTGKAVMETNCQAVEIGK-IRDLGQKYVPDKKATKGI 451
            K T G+ Q G P    + E  + K G    E     V  GK +R+ G         TK  
Sbjct: 335  KNTQGRVQSGVPKKLLLGENVKEKDGNDGEE----LVAGGKGVREDGHSREEQDLLTKQA 394

Query: 452  VLK--DQTSEMITRKEWASGTEKDKKGPRAK--NSQSGLEKEINKALEREKHLEEKE--- 511
             L    Q  +   R    + ++ + K P  K    +  +E       +RE  L +K    
Sbjct: 395  ELTGIPQAQQDTQRTGDGNKSKTNDKNPNGKKFRRRDRMEHMSGPNPQRESILGQKRTQT 454

Query: 512  ISEMDTNQDS-------LMFRGEGKNVRTWKRRARVENNSVDSESLKGATIAN------- 571
             +E +  +D+       +M RGE  N    +R  R   N+   E+     + N       
Sbjct: 455  ATEGEEEKDAKKGRLVVVMERGE-LNYYICERLDRATANARWCEAFPEFEVINSIPRHSD 514

Query: 572  ----VGRKKPLKRKRRSK-----KFEEAWIRVSDSKKIVESSWKEIPGRSFTDYSNKLNR 631
                +   K   R+R  K     +FE  W+      + ++ +W+E         +  + R
Sbjct: 515  HRPIIVNTKGEGRRRSGKGDGSFRFEAWWLEEEGCTEEIQGAWEESWMTGEGGVAGAMRR 574

Query: 632  CLSNLLKWNKDRIGGSIRAAIDRKRDEILR-MEGDDSTANLASIGLAEKELENLLNEEES 691
                + KW+K  + G +   + + R E+ R M    S   +         L  L  ++  
Sbjct: 575  VAGRMRKWHKG-VVGELEGRVKKARAELERCMRSPPSEHKVREEARLRCVLRELEEKKSI 634

Query: 692  YWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLNSSGVWIDDEDEIGNIASSYFK 751
              K RS   WL+ G+RNT++F    + RKK+N +K L    G  + +  E+ N   SYF+
Sbjct: 635  KAKQRSHITWLRKGNRNTRYFMSVVAARKKQNRLKMLRKEDGSDVKEGTELTNYVRSYFQ 694

Query: 752  DLFSSSNPTIVRESFSAIDAEVILNTPVGGEGTRDEIIWNREKKGLFTVKSAYHLAVLLS 811
            +LF++             + E+      GG G   + + +  K                 
Sbjct: 695  ELFTT-------------NVEMQRMNKNGGIGGSSDAVGDLNK----------------- 754

Query: 812  NSKMESPSTVTSHDTFWRKFWKIKALPKAKICVWRIIHDSLPTRVNILKKGIPINYL-CP 871
                         +  W++ WK+      ++  WRI H+SL    N+ ++G  +    C 
Sbjct: 755  -----------CTEDSWKRIWKLACPRNVQMFAWRIKHESLALLTNMQRRGFQLQTTRCF 814

Query: 872  FSRSSEESAKHILWDCKLSKNLW--------KIFIPLTNGLFALNRGSWIPRDYWAWLMD 931
            F   ++E   H+   CK+ K +W        +I +     + A+        DY  W +D
Sbjct: 815  FCGLADEDGAHLFVKCKMVKEVWRELALEKERINLEAITDVHAM-------MDY-LWGLD 874

Query: 932  NLAEEELEIAITILWSIWEYRNKVTHTESKPIYQEIS-RIISSKIDFPKVVSRTYLPKSS 991
                + L + +T  W  W YRNKV   E      E++ R  SS +++ ++ +        
Sbjct: 875  EC--KRLHV-LTFWWLWWSYRNKVRQGELPSTPAEVARRTRSSVLEYRQIYA-------- 934

Query: 992  GKNQPTVKNLASHAIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICVGFKRM 1051
                P  K +++   WKPP +  +K N+D S+    +  G G   R S G  +     R 
Sbjct: 935  ----PGTKKISADE-WKPPGEGVVKFNLDGSFTPGDQHVGWGVAARTSDGDLVAARAGRQ 994

Query: 1052 HNRWSIKALEAKAVLEGLISILSVADLHPSLSSISLLVESDASSVVNLLNEEDEDFTEIS 1086
                     E       +I++ +   L   L  +  + E+D+  ++  L+    D +  +
Sbjct: 995  EYISDPFGAE-------VIAMANAVALAADLGVVQPVFETDSQLLMEALDLRKADSSPYA 998

BLAST of Spg016126 vs. NCBI nr
Match: PWA36168.1 (hypothetical protein CTI12_AA602590 [Artemisia annua])

HSP 1 Score: 210.3 bits (534), Expect = 8.6e-50
Identity = 261/1163 (22.44%), Postives = 453/1163 (38.95%), Query Frame = 0

Query: 128  YLCNFNNSFEKDRIKRGGPWNYDRALLVIEDTQGASRISHTSFRYVNFWVHIHDLPMVCM 187
            +L    +  +  R+   GPW+++R L+V++  +   + + T    V FWV + ++P+   
Sbjct: 2    FLVQLGHDVDLRRVLEDGPWSFERNLVVLKLIENDEQPTETDMTKVPFWVRLINMPLGRR 61

Query: 188  RRKWAEKLGNSLGEFVEADLDEGGSTENTLRIQVKIDVSEPLKRGLMVRIGSKAEETWVK 247
                  ++   +G+ +E  +D+   T+ +  I+VK            VR         V 
Sbjct: 62   DESSVRRVAAKIGDVLE--VDDAYFTKGSKHIRVK---------NTKVR---------VN 121

Query: 248  VTYERLPEFCYGCGIIGHVQQEC------------------------------------- 307
            + YERLP FCY CG++GH ++EC                                     
Sbjct: 122  IQYERLPNFCYWCGLLGHTEKECLTKPFEINGKTFKDWPFHENLRASNSRDSVSLSVASP 181

Query: 308  --------------------EKISNEGE--ENLYGDFMRATPIVGGTPK----------- 367
                                + I NE +  E+   D      + GGT             
Sbjct: 182  LTHPAFNNFQQTTNQRLLIQDNIFNESQINESSNQDLDERKCLSGGTSNYCPTSTLKITG 241

Query: 368  QKTQENKRGNFWGRERGRRGAYQFQNNRQNQK--YNDEKEETWRRRDQSGKSNEENVGVL 427
            QK  +N  G+    + GR+     + + + Q+   N    + W+RR +   + +   G  
Sbjct: 242  QKEPKNSMGSGIELDMGRKIEPHLRGSTKAQQDLENTNLPKLWKRRTREETNTKTTTGT- 301

Query: 428  RPENSTGGRSSPEKETVGKSQGQPTASEVSEQSRMKTGKA-------VMETNCQAVEIGK 487
               NS    + P   ++     Q   +  + Q      K        +MET     E+  
Sbjct: 302  ---NSISIAAPPRPMSILSWNVQGLGNPWTVQHIRSLVKELSPSIIFLMETRLHGSEVTG 361

Query: 488  IRDLGQKY---VPD--KKATKGIVLKDQTSEMITR-KEWASGTEKDKKGPRAKNSQSGLE 547
             R +  +Y   V D  ++A   +V +D      T    W    EK +     ++ ++  E
Sbjct: 362  FRYIFPQYNLLVVDSIRRAGDFVVKEDGNFWRGTGIYGWPRRQEKHRTWALLRSLRTNQE 421

Query: 548  K---------EINKALEREKHLEEKEISEMDTNQDSLMF----------------RGEGK 607
            +         EI  A E+E        +EM   +++  F                 G   
Sbjct: 422  QAWVCFGDFNEIMYAFEKEGQRGSNN-TEMSAFREACSFCNLEDRSAMGVKLTWSNGRRG 481

Query: 608  NVRTWKRRARVENNSVDSESLKGATIANVGR------------KKPLKRKRRSKKFEEAW 667
            N    KR  R   NS   +    A+  N+ R               +K+K R  +FE  W
Sbjct: 482  NENVRKRLDRFLTNSHWFDLYPDASFENLPRIASDHSPIICRLSPMVKKKNRMFRFESMW 541

Query: 668  IRVSDSKKIVESSWK-EIPGRSFTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEI 727
            +R      +V   W   +      D    ++ C + L  WNK R  G ++ +I  K+  +
Sbjct: 542  LRDKSIHGVVRDGWAYGLAAGMQHDPCGIVSECANRLSDWNK-RSFGHVQRSIKSKQRSL 601

Query: 728  LRMEGDDSTANLASIGLAEKELENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERK 787
              ++     +  A      ++++ LL  EE  WK RSR +WL+ GD+NT++FH +AS R+
Sbjct: 602  QTLQSRFDGSTRAEQQALREQIKELLTREELMWKQRSRIEWLREGDKNTRFFHTRASNRQ 661

Query: 788  KRNEIKGLLNSSGVWIDDEDEIGNIASSYFKDLFSSSNP----TIVRE------------ 847
            +RN I  L    G W+++ +E+  + SSYF DLFSSS+P    ++VR+            
Sbjct: 662  RRNSILRLKGPDGRWVEEHNEVCKLVSSYFSDLFSSSSPQGCESVVRDIDRRLTENERQA 721

Query: 848  ---SFSAIDAEVILNTPVGG------------------------EGTRDEIIWNREKKGL 907
                 ++ +   +LNT   G                        +   D + W+    G 
Sbjct: 722  LERPVTSSEVRDLLNTEGDGWNHELMYSLFPHNIASKIGCCFISKSRNDILYWHNNPGGR 781

Query: 908  FTVKSAYHLAVLLSNSKMESPSTVTSHDTFWRKFWKIKALPKAKICVWRIIHDSLPTRVN 967
            F+ KSAY LA+      + + +   S   FWR  WK +   K K+ +WR  ++ +PT  N
Sbjct: 782  FSCKSAYLLALEADEDMVRTTTISNSLIDFWRVVWKARVPSKVKLFMWRAWNNYVPTIDN 841

Query: 968  ILKKGIPINYLCPFSRSSEESAKHILWDCKLSKNLWKIFIPLTNGLFALNRGSWIPRDYW 1027
            +  +G+     C     + E+  H+L+ C ++K++W        G F   +G+   +D+ 
Sbjct: 842  LKSRGLNPTSSCTHCGQTSENLVHVLFKCSVAKDVWN---RCNFGCFYDTQGAITFQDFC 901

Query: 1028 AWLMDNLAEEELEIAITILWSIWEYRNKVTHTESKPIYQEISRIISSKI-DFPKVVSRTY 1087
              +++     E E  + ILW +W  RN+  H +       +  I  S + D+ K   R  
Sbjct: 902  QVILEKFL-AEWETFMMILWGLWTRRNRHFHGQLNGREGNVEVIAKSVLSDYHKANQREN 961

Query: 1088 LPKSSGKNQPTVKNLASHAIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICV 1124
            +  +SG     V N+ +  +W  P    +K+N DA+W  +  K G+G++ R+  G  +  
Sbjct: 962  ISGNSG-----VHNIHA-GMWLRPDVEHIKINCDAAWQKESGKAGLGFVARNYKGEVLFS 1021

BLAST of Spg016126 vs. NCBI nr
Match: KAE8813692.1 (hypothetical protein D1007_09196 [Hordeum vulgare])

HSP 1 Score: 200.3 bits (508), Expect = 8.9e-47
Identity = 254/1118 (22.72%), Postives = 452/1118 (40.43%), Query Frame = 0

Query: 59   KLKITTAERAKVVAIEDEDLEKATEDLQGAIFCRILTPKLINPEVFKTFMPRIWNKEGRV 118
            K+K++  E+ K+VA +                 ++L+ +L +P+  +  + R+W     +
Sbjct: 22   KIKVSMKEKGKIVAAQ--------------AVGKVLSDRLAHPDAIRLSLGRVWCPIKGI 81

Query: 119  RIKAMGRNTYLCNFNNSFEKDRIKRGGPWNYDRALLVIEDTQGASRISHTSFRYVNFWVH 178
              K +G N ++  FN    K R    GPW +++ L+V++      R+    F     WV 
Sbjct: 82   ECKEVGENLFVFTFNQESGKRRALEDGPWMFEKDLMVVDYYDPGKRLEEYDFNETPIWVR 141

Query: 179  IHDLPMVCMRRKWAEKLGNSLGEFVEADLD-EGGSTENTLRIQVKIDVSEPL-------- 238
            I +LP+  M  + AE++GN +G FVEAD+  +G +    LR+++++ + +P+        
Sbjct: 142  IFNLPLGMMNAEAAEEIGNVVGNFVEADVGMDGSALGKCLRVKIRMKLDKPIMRGFTLDD 201

Query: 239  ---------KRGLMVRIGSKAEE-TWVKVTYERLPEFCYGCGIIGHVQQECEKISNEGEE 298
                     KR + +    + E+  W +  YE LP+FCY CG++GH Q+ C     +GE+
Sbjct: 202  EEHEARQQQKRSMKIDDAKEGEDGAWCRFEYEFLPDFCYICGLVGHGQKACSTKLAKGEK 261

Query: 299  NLYGDFMRATPIVGGTPKQKTQENKRGNFWGRERGRRGAYQFQNNRQNQKYNDEKEETWR 358
              +G  +RA        ++K    + G++ GR RG  GA  F   R  +K     +    
Sbjct: 262  TQFGGSLRA-----DMGRRKLYGEEGGSWRGRGRGAGGARSFGVARSYEKSGSGSDSLSW 321

Query: 359  RRDQSGKSNEENVGVLRPENSTGGRSSPEKETVG-KSQGQP----TASEVSEQSRMKTGK 418
            R+D S  +  +     + E  T   S P+    G +  G+P       + +E   +K+  
Sbjct: 322  RKDGSRSTEGKRGDAEKGEEVT---SPPKLPVTGHRDLGRPRKLIMEGQAAEGELIKSRD 381

Query: 419  AVMETNCQAVEI-GKIRDLGQKYVPDKKATKGIVLKDQTSEMIT---RKEWASGTEKDKK 478
                 +   VE+     + G + V D +   G    ++  +M T    ++    T+  KK
Sbjct: 382  GGGRGDLAQVELTHNAHEDGAEVVKDTQLGTG--SNERVQQMPTDMVEEQTHGATDVQKK 441

Query: 479  GP--------------------------RAKNSQSGL-EKEINKALEREKHLEEKEISEM 538
            GP                            K +Q G  E+E +K    E     +  + M
Sbjct: 442  GPGQRRFKRVGRENREDVPCNQTAKKVIGTKRAQDGEGEQEKSKKGRLEIGGGRRPQACM 501

Query: 539  DTNQDSLMFRG------EGKNVRTWKRRARVENNSVDSESLKGATIANV-------GRKK 598
            D  +++L+  G       G    TW+   R E +S   E L  AT ANV       G   
Sbjct: 502  DRFREALVHAGLCDIGYTGDRF-TWRNHNR-ELHSYICERLDRAT-ANVDWCAKFPGFSV 561

Query: 599  PLKRKRRSK-----------------------KFEEAWIRVSDSKKIVESSWKE--IPGR 658
              +R R S                        +FE  W++     ++++ +W+E  + G 
Sbjct: 562  VHERPRHSDHRPLVINTVGDVVKGGGGGDRGFRFEAWWLQEDGCAEVIQDAWEEGQVEG- 621

Query: 659  SFTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEILR-MEGDDSTANLASIGLAEK 718
               +    + +  + + +W+K+ I G +   + + + E+ R M    S A +   G    
Sbjct: 622  --CNVEQTMRKVAARMRRWHKEEI-GELTGRLKKAQAELGRCMCAPVSEAKIREEGRLRG 681

Query: 719  ELENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLNSSGVWIDDED 778
               +L  ++ +  K RS   WL+ G+RNT++F   AS R+K+N IKGL+   G+ + + D
Sbjct: 682  VDNDLEEKKNTRAKQRSHIAWLKDGNRNTRYFMAVASARRKKNRIKGLVKEDGMVVKEGD 741

Query: 779  EIGNIASSYFKDLFSSSNPTIVRESFSAIDAEVILNTPVGGEGTRDEIIWNREKKGLFTV 838
             + N   SYF                                            +GLFT 
Sbjct: 742  ALTNYVCSYF--------------------------------------------QGLFTS 801

Query: 839  KSAYHLAVLLSNSKMESPSTVTSHDTFWRKFWKIKALPK-AKICVWRIIHDSLPTRVNIL 898
              +  LA LL   +                  ++ A P+  ++  WR+ H+SL  R N+ 
Sbjct: 802  HMSGRLADLLDTVQP-----------------RVDAGPRNIQMFTWRLKHESLVLRSNLK 861

Query: 899  KKGIPI-NYLCPFSRSSEESAKHILWDCKLSKNLWK-IFIPLTNGLFALNRGSWIPRDYW 958
            K+GIP+ +  C F   +EE   H+   CK  K  W+ + +    GL           D+ 
Sbjct: 862  KRGIPVEDATCLFCGKAEEDGGHLFIKCKHVKGGWRALEMEQERGLLQEITSVHQALDF- 921

Query: 959  AWLMDNLAEEELEIAITILWSIWEYRNKVTHTESKPIYQEISRIISSKIDFPKVVSRTYL 1018
             W    L+E +    +T  W  W  RNK+   E     +E++R   +      V+    +
Sbjct: 922  LW---GLSEIKRMHILTFSWLWWSNRNKLREGELPEKAEEVARRTRA-----NVMEYMQI 981

Query: 1019 PKSSGKNQPTVKNLASHAIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICVG 1078
             ++  K++        H  WKPPPD ++K+N D S+         G + RD +G  +   
Sbjct: 982  FRAPDKDK-------CHTKWKPPPDGTIKINADGSFIPGQTDSSWGVVARDCNGDVLLAR 1024

BLAST of Spg016126 vs. NCBI nr
Match: GAU41525.1 (hypothetical protein TSUD_140560 [Trifolium subterraneum])

HSP 1 Score: 199.5 bits (506), Expect = 1.5e-46
Identity = 200/822 (24.33%), Postives = 345/822 (41.97%), Query Frame = 0

Query: 56  ELEKLKIT-TAERAKVVAIEDEDLEKATEDLQGAIFCRILTPKLINPEVFKTFMPRIWNK 115
           E EK  I+ T +    + +E E++ +  E  +  +  ++ T    N   FK  + + W  
Sbjct: 2   EREKQIISPTEDEEDCITVEAEEICEEEETFKRTLVGKLWTENPYNVRAFKQTIAQAWRL 61

Query: 116 EGRVRIKAMGRNTYLCNFNNSFEKDRIKRGGPWNYDRALLVIEDTQGASRISHTSFRYVN 175
           +  + ++ +  N +L  F    + D + R GPW++DR LL++    G  + S      VN
Sbjct: 62  KNSIEVQDLEENLFLFRFTTKKDADTVLRNGPWSFDRNLLILHRVSGEEQPSDLDMHNVN 121

Query: 176 FWVHIHDLPMVCMRRKWAEKLGNSLGEFVEADLDEGGSTENTLRIQVKIDVSEPLKRGLM 235
           FWV ++DLP        A+KLGN +G F E D  +   T   LR++  +D+ +PLKRG  
Sbjct: 122 FWVRVYDLPFKLRSEAMAKKLGNIMGNFEEVDPKDANRTGRFLRLKASLDLRKPLKRGTK 181

Query: 236 VRIGSKAEETWVKVTYERLPEFCYGCGIIGHVQQECEKISNEGEEN---------LYGDF 295
           ++   K    WV   YERLP FC+ CG IGH  +ECE + +  E N          YG +
Sbjct: 182 IKFQDK--NMWVDFKYERLPNFCFACGKIGHQMKECEDVEDVDENNYSDIAEKSQAYGPW 241

Query: 296 MRATPI--VGGTPKQKTQENKRGNFWGRERGRRGAYQFQNNRQNQKYNDEKEETWRRRDQ 355
           +RA+P+  +   P++                +    Q +  +  ++  D++      +  
Sbjct: 242 LRASPLPRIFEEPRKDASSGSCSKNLFPSSSQSKGGQSEGKKDKEQEVDQQPVVGIEKQL 301

Query: 356 SGKSNEENVGVLRPENSTGGRSSPEKETVGKSQGQPTASEVSEQSRMKTGKAVMETNCQ- 415
                  N+ V     S G  +      VGK+      S+  +  R K+ K V     + 
Sbjct: 302 VPTKATNNLDVEDVAESLGAVAISTSFVVGKTTKSQGGSKGRKWVRNKSSKPVKAQTARK 361

Query: 416 -AVEIGKIRDLGQKYVPDKKATKGIVLKDQTSE---MITRKE------------WASGTE 475
            A E+GK R+L    + + +A   ++  +       M TR +            ++SG  
Sbjct: 362 LAKELGK-RNLVDVAISEVRALSRLIRLENPQVVFLMETRLKVPEIDRLKFKLGFSSGLA 421

Query: 476 KDKKGPRAKNS-------------------------------------QSGLEKEINKA- 535
            D KG   + +                                      +G+  E  K  
Sbjct: 422 IDCKGVGRERAGGLALFWKDHMDITIKSYSLNHIHGQCVDVETNEPWDLTGIYAEEKKGG 481

Query: 536 -LEREKHLEEKEISEMDTNQDSLMFRGEGKNVRTWKRRARVENNSVDSESLKGATIANVG 595
            +  +  LE    + +++  + L F G      TW      ++N    +    ++   + 
Sbjct: 482 LVRTQGQLEIGRQAILESRLNDLGFEG---YPLTWSNGRNSDDNKQCRQDRAMSSDEFIN 541

Query: 596 RKKPL---------------------------KRKRRSK---KFEEAWIRVSDSKKIVES 655
           R  P+                           +R+RR K   +FEE+W   +  + ++++
Sbjct: 542 RFSPIHVLHLPRYGSDHVVLVITLEAPTHRDQRRRRRRKRLFRFEESWTSNARCEPLIQA 601

Query: 656 SWKEIPGRSFTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEILRME-GDDSTANL 715
            W + P  SF+D   +L R + N L    D   GSI   I R    I   +  D+S  ++
Sbjct: 602 CWSQ-PCLSFSDKLGRL-RDMGNEL---GDHSVGSIHKEIVRIEKLIQNHDMWDESETSI 661

Query: 716 ASIGLAEKELENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLNSS 775
                 E  LE LL EEE+ W+ RSR  WL+ GD+NTK+FH KAS+R+K NEIK L +  
Sbjct: 662 QRFKALEHTLEELLKEEETMWRQRSRALWLKDGDKNTKFFHGKASQRRKVNEIKKLKDEH 721

Query: 776 GVWIDDEDEIGNIASSYFKDLFSSSNPTIVRESFSAIDAEVILNTPVGGEGTRDEIIWNR 779
           G+W   E+++  +   YFK+LF+S++P+ V ++   +  ++ L          D +  + 
Sbjct: 722 GLWWRGEEKVEKLMLDYFKELFTSASPSNVEQTCGVVREKLSL---------EDRVRCDE 781

BLAST of Spg016126 vs. NCBI nr
Match: GAU41525.1 (hypothetical protein TSUD_140560 [Trifolium subterraneum])

HSP 1 Score: 99.0 bits (245), Expect = 2.8e-16
Identity = 63/206 (30.58%), Postives = 101/206 (49.03%), Query Frame = 0

Query: 689  IASSYFKDLFSSSNPTIVRESFSAIDAEVILNTPVGGEGTRDEIIWNREKKGLFTVKSAY 748
            + S    D     N  I+ + F+  +A  I++TP+      D+IIW+ E+ G+++V+ AY
Sbjct: 1295 LVSELIDDQTRQWNRAIIFKFFTNDEALKIVSTPLSLNLPADKIIWHWERDGVYSVRPAY 1354

Query: 749  HLAVLLSNSKMESPSTVTSHDTFWRKFWKIKALPKAKICVWRIIHDSLPTRVNILKKGIP 808
            HL     +  +  PS+ +  D+ W+K WK     K K  + R+  + LPTR N+ KKGI 
Sbjct: 1355 HLLCDARDINLPGPSS-SGDDSLWKKIWKAPIPNKVKNFMSRLAKNILPTRDNLKKKGIS 1414

Query: 809  INYLCPFSRSSEESAKHILWDCKLSKNLWKIFIPLTNGLFALNRGSWIPR--DYWAWLMD 868
            ++  CP   +  E+A H+   C +          L   LFA   G   P   D   WL++
Sbjct: 1415 LDTSCPLCHNDSENAHHLFMHCNM----------LKLALFASPLGCHPPMNVDLNCWLLE 1474

Query: 869  NL-AEEEL--EIAITILWSIWEYRNK 890
             L   ++L  ++  TILW  W  RN+
Sbjct: 1475 WLNCSDKLGAQLFCTILWKFWFARNQ 1489

HSP 2 Score: 195.7 bits (496), Expect = 2.2e-45
Identity = 174/663 (26.24%), Postives = 277/663 (41.78%), Query Frame = 0

Query: 502  WKRRARVENNSV-DSESLKGATIANVGRKKPLKRKRRSKKFEEAWIRVSDSKKIVESSWK 561
            WKRR  +   +V D  S     I    R    +   R  +FE  W   S  ++IVE  W 
Sbjct: 31   WKRRFALSKAAVLDYSSSDHLPILLQVRIFLPRHSPRLFRFENTWGAESGCREIVEEIWS 90

Query: 562  EIPGRSFTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEILRMEGDDSTANLASIG 621
            +    S      KL  C   L +W  D +    +  ID  +  +  + G     +  S  
Sbjct: 91   D---PSLGSVLEKLQFCSVRLGEWG-DNLRKLYKQEIDDCKRVMGLLRGKRDAHSRESFR 150

Query: 622  LAEKELENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLNSSGVWI 681
            +A+ +  +LL  +ESYW+ R++E WL+ GD+NTK+FH KA+ R+++N I  L + +G W 
Sbjct: 151  MAKSKFLDLLRRKESYWRQRAKEFWLKEGDQNTKFFHRKATIRQRKNRIDRLKDDNGNWC 210

Query: 682  DDEDEIGNIASSYFKDLFSSS--------------------------------------- 741
            D    +  + ++YFK++F+S+                                       
Sbjct: 211  DWSTGLEAMITNYFKNIFTSNAGSSNDIIDLVPALVTDDQNVDLMKPWLPDVAFPMVTTT 270

Query: 742  -------------------NPTIVRESFSAIDAEVILNTPVGGEGTRDEIIWNREKKGLF 801
                               N  +V   F+  D ++IL+ P+    + D   W  +K+G +
Sbjct: 271  FDFNFGVYMVSDLITDGAWNIDLVNRVFNERDRKLILSIPLRRSSSIDLQFWKHDKRGSY 330

Query: 802  TVKSAYHLAVLLSNSKMESPSTVTSHDTFWRKFWKIKALPKAKICVWRIIHDSLPTRVNI 861
            +V SAY L       + +   T   +  FW+K W I A PK +  +WR +  SLPTR  +
Sbjct: 331  SVSSAYKL-------QTQESDTAVGNKKFWKKLWSIPAAPKIRNFLWRAVSGSLPTRSRL 390

Query: 862  LKKGIPINYLCPFSRSSEESAKHILWDCKLSKNLWKIFIPLTNGLFALNRGSWIP--RDY 921
              + +P   +CP      ES KH+L +C  ++++W           A + G + P    +
Sbjct: 391  CSRHVPTTEVCPLCNVDCESVKHVLVECSFARHVW----------LASHLGWFSPSTHSF 450

Query: 922  WAWLMDNLA---EEELEIAITILWSIWEYRNKVTHTESKPIYQEI---SRIISSKIDFPK 981
              WLM  LA     +      + WSIWE RN V    ++P           +  + D   
Sbjct: 451  QDWLMRALAIFNPNDSATLAYLCWSIWEERNSVVWKSAQPAANSCRLRGLRLKREWDDAL 510

Query: 982  VVSRTYLPKSSGKNQPTVKNLASHAIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSS 1041
            V  R  +P       PT  +L +   WK P    +KLNVD + N      G+G ++R+  
Sbjct: 511  VSIRAAIP-------PTA-DLPTPQEWKKPGPNWVKLNVDIATNVNSGWTGIGMVVRNEM 570

Query: 1042 GSSICVGFKRMHNRWSIKALEAKAVLEGLISILSVADLHPSLSSISLLVESDASSVVNLL 1096
            GS +     RM   +S    E   V E L  I          +  +++VESD   VVN+L
Sbjct: 571  GSFVACKISRMVGLFSPTIAEIMGVREALSWI-------KDNNWQNVIVESDNLQVVNVL 630

BLAST of Spg016126 vs. ExPASy TrEMBL
Match: A0A803P5M6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.3e-55
Identity = 278/1318 (21.09%), Postives = 482/1318 (36.57%), Query Frame = 0

Query: 49   MEEAMANELEKLKITTAERAKVVAIEDEDLEKATEDLQGAIFCRILTPKLINPEVFKTFM 108
            M+  M     K+ +T  E + V    D +       +   ++ +ILT K +     +  M
Sbjct: 1    MDPLMNKMKTKINLTEDEES-VFQFHDTESTSPLLSVDTVLYAKILTKKKVWLSTLQNQM 60

Query: 109  PRIWNKEGRVRIK-AMGRNTYLCNFNNSFEKDRIKRGGPWNYDRALLVIEDTQGASRISH 168
               W  +GR  +K +   + ++  F    +K R+    P+++    +V+   +     + 
Sbjct: 61   AEHW--DGRFPVKISEAMDMFMLTFGCEGDKIRVLDREPYHFQNHHIVLYTPEVGKNFTS 120

Query: 169  TSFRYVNFWVHIHDLPMVCMRRKWAEKLGNSLGEF---VEADLDEGGSTENTLRIQVKID 228
                +  FWV I+ LP +   R  A  LGN +GE+    E  L+EG      LR++V +D
Sbjct: 121  DDLTFTPFWVQIYRLPFLSKTRTLAIALGNIIGEYKDVFEDSLNEGWGP--FLRVRVLLD 180

Query: 229  VSEPLKRGLMVRIGSKAEETWVKVTYERLPEFCYGCGIIGHVQQEC----EKISNEGEEN 288
            VS+PLKRG M+ +    ++ WV   YERLPE+C  CG+IGH   +C    EK+ N  E  
Sbjct: 181  VSKPLKRGRMISLSHVKDKFWVDFRYERLPEYCMECGVIGHPYNKCSVFLEKLDNGEEPA 240

Query: 289  L-YGDFMRATPIVGGTPKQKTQENKRGNFWG--------------RERGRRG-------- 348
            L Y  F++ + +      +   +  +G+ W                +  +RG        
Sbjct: 241  LEYQPFIKGSTLPTSGYDRYRTDFAKGDAWPLITRLAKKSLTSAIPQLAKRGQPHPRILF 300

Query: 349  AYQFQNNRQNQKYNDEKEETWRRRDQSGKSNEENVGVLRPENSTGGRSSPEKETVGKSQG 408
            + +  N    +  N+  ++    R    K   +   V+   ++    +    + +     
Sbjct: 301  SGESSNTNNFEDSNNNLDDIVAARSMVPKDTIKPPSVVFHPSTLLSSAQNLHKGMNAPAT 360

Query: 409  QPTASEVSEQSRMKTGKAVMETNCQAVEI---GKIRDLGQKYVPDKKATKGIVLKDQTSE 468
            + + +  S+ S +     V  T            + D+  K      AT  +  K     
Sbjct: 361  KDSPTNFSDLSSIYMPAFVPNTTNTFATYPPQTSLTDIRGKQPMPAHATNALPPK-VAPA 420

Query: 469  MITRKEWASGTEKDKKGPRAKNSQSGLEKEINKALER------------------EKHLE 528
             I+      GTE      ++K    GL   + + L+R                  + HL 
Sbjct: 421  TISPAVMHWGTENTNPNVQSKRQNEGL--SLRQTLKRCRGTIPNDAASTLSVGEVDHHLV 480

Query: 529  EKEISEMDTNQDS--------------LMFRGE-------GKNVRTWKRR---------- 588
              ++ +   ++D+              + F G+         N    K R          
Sbjct: 481  SIDLMDSMGDKDNSVRGLSDPPHSSMKIPFHGDKFTWIKGRHNPNALKERLDWCFLNDLW 540

Query: 589  ---------ARVENNSVDSESLKGATIANVGRKKPLKRKRRSKKFEEAWIRVSDSKKIVE 648
                     A ++  S D  ++   TI  +   +    ++   +FE+ W++  D+  I+ 
Sbjct: 541  ETIFKPITTAHLDYYSSDHRAI-AVTIEYITSNQQQPTRKTRFRFEKIWLKEPDAASIIR 600

Query: 649  SSWKEIPGRSFTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEILRME--GDDSTA 708
            + W +        + + L  C   L +W+  +  G+++  I   + ++ R+    D S +
Sbjct: 601  AHWSDTDAGGMDIFLSNLQSCTDALQQWHIRKF-GNMKKKITNMQQQVSRLNNPADRSVS 660

Query: 709  NLASIGLAEKELENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLN 768
             +  +  +E  L+ LL +EE+YW  RSR DWLQ GD+NT +FH  A+ RK +N IK L+N
Sbjct: 661  TMDELKDSEAILDELLEQEETYWHQRSRVDWLQCGDQNTSFFHAHATSRKSKNVIKSLIN 720

Query: 769  SSGVWIDDEDEIGNIASSYFKDLFSSS--------------------------------- 828
            + G+ +  + E+ N+   Y+  LF+S                                  
Sbjct: 721  TQGLSVSSKAEMTNVICDYYTSLFASDGVDPDSLNDILTTVPASITHEMNQSLTTPFTSN 780

Query: 829  ------------------------------------------------------------ 888
                                                                        
Sbjct: 781  EVLNNDKSVMSFSPNTSLAAQDFFARTLHMPITECHERYLGLPSYSGRDKQELFSSIKDK 840

Query: 889  ------------------------------------------------------------ 948
                                                                        
Sbjct: 841  VWKLLHAWNEKIFSVGGKEVLLKAVVQSIPTYAMSCFKLTKKFCDQLERDGIHVDSGKDP 900

Query: 949  -------------------------------NPTIVRESFSAIDAEVILNTPVGGEGTRD 1008
                                           N  ++   F  ID + IL+ P+      D
Sbjct: 901  WIPSHNDFKPVSYLGSASLPVSHFITNNRVWNVPLLTSYFQQIDIDRILSIPLAYFAGTD 960

Query: 1009 EIIWNREKKGLFTVKSAYHLAVLLSNSKMESPSTVTSHDTFWRKFWKIKALPKAKICVWR 1068
             ++W+    G+++VK+ +HLA  L +    S ST      +W+ FW +K  PK +I  W+
Sbjct: 961  RLVWHHSPNGIYSVKTGFHLATTLED--QNSSSTSNKQSEWWKFFWNLKLPPKIRIFAWK 1020

Query: 1069 IIHDSLPTRVNILKKGIPINYLCPFSRSSEESAKHILWDCKLSKNLWKIFIPLTNGLFAL 1083
            +  + LPT V + K+ +  +  C    S+ ES  H L+ CK +K++WK+     +   A 
Sbjct: 1021 VFQNILPTAVALFKRKVLDSGECSLCTSNWESIGHALFSCKHAKDIWKLSRFRIDFSQAH 1080

BLAST of Spg016126 vs. ExPASy TrEMBL
Match: A0A2U1KHJ0 (CCHC-type domain-containing protein OS=Artemisia annua OX=35608 GN=CTI12_AA602590 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.2e-50
Identity = 261/1163 (22.44%), Postives = 453/1163 (38.95%), Query Frame = 0

Query: 128  YLCNFNNSFEKDRIKRGGPWNYDRALLVIEDTQGASRISHTSFRYVNFWVHIHDLPMVCM 187
            +L    +  +  R+   GPW+++R L+V++  +   + + T    V FWV + ++P+   
Sbjct: 2    FLVQLGHDVDLRRVLEDGPWSFERNLVVLKLIENDEQPTETDMTKVPFWVRLINMPLGRR 61

Query: 188  RRKWAEKLGNSLGEFVEADLDEGGSTENTLRIQVKIDVSEPLKRGLMVRIGSKAEETWVK 247
                  ++   +G+ +E  +D+   T+ +  I+VK            VR         V 
Sbjct: 62   DESSVRRVAAKIGDVLE--VDDAYFTKGSKHIRVK---------NTKVR---------VN 121

Query: 248  VTYERLPEFCYGCGIIGHVQQEC------------------------------------- 307
            + YERLP FCY CG++GH ++EC                                     
Sbjct: 122  IQYERLPNFCYWCGLLGHTEKECLTKPFEINGKTFKDWPFHENLRASNSRDSVSLSVASP 181

Query: 308  --------------------EKISNEGE--ENLYGDFMRATPIVGGTPK----------- 367
                                + I NE +  E+   D      + GGT             
Sbjct: 182  LTHPAFNNFQQTTNQRLLIQDNIFNESQINESSNQDLDERKCLSGGTSNYCPTSTLKITG 241

Query: 368  QKTQENKRGNFWGRERGRRGAYQFQNNRQNQK--YNDEKEETWRRRDQSGKSNEENVGVL 427
            QK  +N  G+    + GR+     + + + Q+   N    + W+RR +   + +   G  
Sbjct: 242  QKEPKNSMGSGIELDMGRKIEPHLRGSTKAQQDLENTNLPKLWKRRTREETNTKTTTGT- 301

Query: 428  RPENSTGGRSSPEKETVGKSQGQPTASEVSEQSRMKTGKA-------VMETNCQAVEIGK 487
               NS    + P   ++     Q   +  + Q      K        +MET     E+  
Sbjct: 302  ---NSISIAAPPRPMSILSWNVQGLGNPWTVQHIRSLVKELSPSIIFLMETRLHGSEVTG 361

Query: 488  IRDLGQKY---VPD--KKATKGIVLKDQTSEMITR-KEWASGTEKDKKGPRAKNSQSGLE 547
             R +  +Y   V D  ++A   +V +D      T    W    EK +     ++ ++  E
Sbjct: 362  FRYIFPQYNLLVVDSIRRAGDFVVKEDGNFWRGTGIYGWPRRQEKHRTWALLRSLRTNQE 421

Query: 548  K---------EINKALEREKHLEEKEISEMDTNQDSLMF----------------RGEGK 607
            +         EI  A E+E        +EM   +++  F                 G   
Sbjct: 422  QAWVCFGDFNEIMYAFEKEGQRGSNN-TEMSAFREACSFCNLEDRSAMGVKLTWSNGRRG 481

Query: 608  NVRTWKRRARVENNSVDSESLKGATIANVGR------------KKPLKRKRRSKKFEEAW 667
            N    KR  R   NS   +    A+  N+ R               +K+K R  +FE  W
Sbjct: 482  NENVRKRLDRFLTNSHWFDLYPDASFENLPRIASDHSPIICRLSPMVKKKNRMFRFESMW 541

Query: 668  IRVSDSKKIVESSWK-EIPGRSFTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEI 727
            +R      +V   W   +      D    ++ C + L  WNK R  G ++ +I  K+  +
Sbjct: 542  LRDKSIHGVVRDGWAYGLAAGMQHDPCGIVSECANRLSDWNK-RSFGHVQRSIKSKQRSL 601

Query: 728  LRMEGDDSTANLASIGLAEKELENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERK 787
              ++     +  A      ++++ LL  EE  WK RSR +WL+ GD+NT++FH +AS R+
Sbjct: 602  QTLQSRFDGSTRAEQQALREQIKELLTREELMWKQRSRIEWLREGDKNTRFFHTRASNRQ 661

Query: 788  KRNEIKGLLNSSGVWIDDEDEIGNIASSYFKDLFSSSNP----TIVRE------------ 847
            +RN I  L    G W+++ +E+  + SSYF DLFSSS+P    ++VR+            
Sbjct: 662  RRNSILRLKGPDGRWVEEHNEVCKLVSSYFSDLFSSSSPQGCESVVRDIDRRLTENERQA 721

Query: 848  ---SFSAIDAEVILNTPVGG------------------------EGTRDEIIWNREKKGL 907
                 ++ +   +LNT   G                        +   D + W+    G 
Sbjct: 722  LERPVTSSEVRDLLNTEGDGWNHELMYSLFPHNIASKIGCCFISKSRNDILYWHNNPGGR 781

Query: 908  FTVKSAYHLAVLLSNSKMESPSTVTSHDTFWRKFWKIKALPKAKICVWRIIHDSLPTRVN 967
            F+ KSAY LA+      + + +   S   FWR  WK +   K K+ +WR  ++ +PT  N
Sbjct: 782  FSCKSAYLLALEADEDMVRTTTISNSLIDFWRVVWKARVPSKVKLFMWRAWNNYVPTIDN 841

Query: 968  ILKKGIPINYLCPFSRSSEESAKHILWDCKLSKNLWKIFIPLTNGLFALNRGSWIPRDYW 1027
            +  +G+     C     + E+  H+L+ C ++K++W        G F   +G+   +D+ 
Sbjct: 842  LKSRGLNPTSSCTHCGQTSENLVHVLFKCSVAKDVWN---RCNFGCFYDTQGAITFQDFC 901

Query: 1028 AWLMDNLAEEELEIAITILWSIWEYRNKVTHTESKPIYQEISRIISSKI-DFPKVVSRTY 1087
              +++     E E  + ILW +W  RN+  H +       +  I  S + D+ K   R  
Sbjct: 902  QVILEKFL-AEWETFMMILWGLWTRRNRHFHGQLNGREGNVEVIAKSVLSDYHKANQREN 961

Query: 1088 LPKSSGKNQPTVKNLASHAIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICV 1124
            +  +SG     V N+ +  +W  P    +K+N DA+W  +  K G+G++ R+  G  +  
Sbjct: 962  ISGNSG-----VHNIHA-GMWLRPDVEHIKINCDAAWQKESGKAGLGFVARNYKGEVLFS 1021

BLAST of Spg016126 vs. ExPASy TrEMBL
Match: A0A2N9GD63 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS25220 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 3.3e-47
Identity = 180/691 (26.05%), Postives = 317/691 (45.88%), Query Frame = 0

Query: 83  EDLQGAIFCRILTPKLINPE-VFKTFMPRIWNKEGRVRIKAMGRNTYLCNFNNSFEKDRI 142
           ED Q  +  R +T + +N E V +TF P +W       ++ +G N  +  F +  + +R+
Sbjct: 27  EDDQFYLAARFMTGRFLNIESVVRTFRP-LWRTVRGFTVRDLGHNVLVFAFEDVTDLERV 86

Query: 143 KRGGPWNYDRALLVIEDTQGASRISHTSFRYVNFWVHIHDLPMVCMRRKWAEKLGNSLGE 202
           ++G PW+YD+ L+  +     + ++     YV+FWV I++LP+  M+R++A  LG+++GE
Sbjct: 87  RQGEPWSYDKHLVSFQRVDIDTEVTEMQCGYVSFWVQIYNLPIGRMKREFAMALGSAVGE 146

Query: 203 FVE-ADLDEGGSTENTLRIQVKIDVSEPLKRGLMVRIGSKAEETWVKVTYERLPEFCYGC 262
             + A+ ++    E  +RI+VK+D+S+PL RG   R+ S   ETW+   YERLP FCY C
Sbjct: 147 VEQVAESEKEKGCEGCMRIRVKVDISKPLCRGRKARLAS-GRETWISFKYERLPIFCYWC 206

Query: 263 GIIGHVQQECEK-ISNEG----EENLYGDFMRATPIVGGTPKQKTQENKRGNFWGRERGR 322
           G + H +++CE  + ++G    EE  YG ++RA P+    P ++ +    GN    +   
Sbjct: 207 GCLTHGEKDCEMWLKHKGEMRREEQQYGAWLRA-PV--EKPVKRIEIKVAGNPMCLDGVI 266

Query: 323 RGAYQFQNNRQNQKYNDEKEETWRRRDQSGKSNEENVGVLRPENSTGGRSSPEKETVGKS 382
                 ++ R+ Q    + + +W    +   S +EN+G  +  N+    +  E +  G++
Sbjct: 267 NHLLLPRSRRKFQAVKLQVQTSWLNGSRRKGSGKENLG-SQSLNNVDLNAMHEVKVHGET 326

Query: 383 QGQPTASEVSEQSRMKTGKAVMETNCQAVEIGKIRDLGQKYVPDKKATKGIVLKDQTSEM 442
           +G      V       TG   +        IG   ++    V  KK  +G+   +     
Sbjct: 327 RG--GWKRVPRVIATITGDGELTKLAGVKRIGDWVEVQSPDVHGKKKGRGLAAMEGVE-- 386

Query: 443 ITRKEWA-SGTEKDKKGPRAKNSQSGLEK-------------EINKALEREKHLEEKEIS 502
           +   +W  +G        R   S + L+K             + N+ L  ++ L E   S
Sbjct: 387 VGELQWRFTGFYGHPVAHRRHESWALLDKLHSMHAVPWLLMGDFNEILSSDERLGESAGS 446

Query: 503 EMD-------TNQDSLMFRGEGKNVRTWKR--------RARVENNSVDSESLKGATIANV 562
           + +        N+  L+  G      TW+         + R++        +   T+  +
Sbjct: 447 QRNMYELGEVLNRCGLVDLGYRGYPFTWENCREAGANVQKRLDRAVASVSWMTMFTLCTI 506

Query: 563 GR------------------KKPLKRKRRSKKFEEAWIRVSDSKKIVESSWKEIP--GRS 622
                                   + KRR +KFEE W    + +KI+   W +    G  
Sbjct: 507 DHVPTSYSDHVPILLHTDLGSNSSRHKRRPRKFEEKWAIHPECEKIIHDVWGQADPIGSP 566

Query: 623 FTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEILRMEGDDSTANLASIGLAEKEL 682
                 K+ +C  +L +W K           D+       + G+    N  +I   ++E+
Sbjct: 567 MFVVCEKIKQCRESLFRWYKGMSSEFHLKIQDKTMSLSNLIAGNLLGVNAEAITAIKQEI 626

Query: 683 ENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLNSSGVWIDDEDEI 718
             LL  EE +W+ RSR  WL+ GDRNTK+FH  A++RKK N I+G  N + VW D E ++
Sbjct: 627 NQLLLSEELHWRQRSRMVWLEVGDRNTKYFHQYANQRKKTNGIQGFRNEANVWCDSEQQM 686

BLAST of Spg016126 vs. ExPASy TrEMBL
Match: A0A2Z6NZV1 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_140560 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.4e-47
Identity = 200/822 (24.33%), Postives = 345/822 (41.97%), Query Frame = 0

Query: 56  ELEKLKIT-TAERAKVVAIEDEDLEKATEDLQGAIFCRILTPKLINPEVFKTFMPRIWNK 115
           E EK  I+ T +    + +E E++ +  E  +  +  ++ T    N   FK  + + W  
Sbjct: 2   EREKQIISPTEDEEDCITVEAEEICEEEETFKRTLVGKLWTENPYNVRAFKQTIAQAWRL 61

Query: 116 EGRVRIKAMGRNTYLCNFNNSFEKDRIKRGGPWNYDRALLVIEDTQGASRISHTSFRYVN 175
           +  + ++ +  N +L  F    + D + R GPW++DR LL++    G  + S      VN
Sbjct: 62  KNSIEVQDLEENLFLFRFTTKKDADTVLRNGPWSFDRNLLILHRVSGEEQPSDLDMHNVN 121

Query: 176 FWVHIHDLPMVCMRRKWAEKLGNSLGEFVEADLDEGGSTENTLRIQVKIDVSEPLKRGLM 235
           FWV ++DLP        A+KLGN +G F E D  +   T   LR++  +D+ +PLKRG  
Sbjct: 122 FWVRVYDLPFKLRSEAMAKKLGNIMGNFEEVDPKDANRTGRFLRLKASLDLRKPLKRGTK 181

Query: 236 VRIGSKAEETWVKVTYERLPEFCYGCGIIGHVQQECEKISNEGEEN---------LYGDF 295
           ++   K    WV   YERLP FC+ CG IGH  +ECE + +  E N          YG +
Sbjct: 182 IKFQDK--NMWVDFKYERLPNFCFACGKIGHQMKECEDVEDVDENNYSDIAEKSQAYGPW 241

Query: 296 MRATPI--VGGTPKQKTQENKRGNFWGRERGRRGAYQFQNNRQNQKYNDEKEETWRRRDQ 355
           +RA+P+  +   P++                +    Q +  +  ++  D++      +  
Sbjct: 242 LRASPLPRIFEEPRKDASSGSCSKNLFPSSSQSKGGQSEGKKDKEQEVDQQPVVGIEKQL 301

Query: 356 SGKSNEENVGVLRPENSTGGRSSPEKETVGKSQGQPTASEVSEQSRMKTGKAVMETNCQ- 415
                  N+ V     S G  +      VGK+      S+  +  R K+ K V     + 
Sbjct: 302 VPTKATNNLDVEDVAESLGAVAISTSFVVGKTTKSQGGSKGRKWVRNKSSKPVKAQTARK 361

Query: 416 -AVEIGKIRDLGQKYVPDKKATKGIVLKDQTSE---MITRKE------------WASGTE 475
            A E+GK R+L    + + +A   ++  +       M TR +            ++SG  
Sbjct: 362 LAKELGK-RNLVDVAISEVRALSRLIRLENPQVVFLMETRLKVPEIDRLKFKLGFSSGLA 421

Query: 476 KDKKGPRAKNS-------------------------------------QSGLEKEINKA- 535
            D KG   + +                                      +G+  E  K  
Sbjct: 422 IDCKGVGRERAGGLALFWKDHMDITIKSYSLNHIHGQCVDVETNEPWDLTGIYAEEKKGG 481

Query: 536 -LEREKHLEEKEISEMDTNQDSLMFRGEGKNVRTWKRRARVENNSVDSESLKGATIANVG 595
            +  +  LE    + +++  + L F G      TW      ++N    +    ++   + 
Sbjct: 482 LVRTQGQLEIGRQAILESRLNDLGFEG---YPLTWSNGRNSDDNKQCRQDRAMSSDEFIN 541

Query: 596 RKKPL---------------------------KRKRRSK---KFEEAWIRVSDSKKIVES 655
           R  P+                           +R+RR K   +FEE+W   +  + ++++
Sbjct: 542 RFSPIHVLHLPRYGSDHVVLVITLEAPTHRDQRRRRRRKRLFRFEESWTSNARCEPLIQA 601

Query: 656 SWKEIPGRSFTDYSNKLNRCLSNLLKWNKDRIGGSIRAAIDRKRDEILRME-GDDSTANL 715
            W + P  SF+D   +L R + N L    D   GSI   I R    I   +  D+S  ++
Sbjct: 602 CWSQ-PCLSFSDKLGRL-RDMGNEL---GDHSVGSIHKEIVRIEKLIQNHDMWDESETSI 661

Query: 716 ASIGLAEKELENLLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLNSS 775
                 E  LE LL EEE+ W+ RSR  WL+ GD+NTK+FH KAS+R+K NEIK L +  
Sbjct: 662 QRFKALEHTLEELLKEEETMWRQRSRALWLKDGDKNTKFFHGKASQRRKVNEIKKLKDEH 721

Query: 776 GVWIDDEDEIGNIASSYFKDLFSSSNPTIVRESFSAIDAEVILNTPVGGEGTRDEIIWNR 779
           G+W   E+++  +   YFK+LF+S++P+ V ++   +  ++ L          D +  + 
Sbjct: 722 GLWWRGEEKVEKLMLDYFKELFTSASPSNVEQTCGVVREKLSL---------EDRVRCDE 781

BLAST of Spg016126 vs. ExPASy TrEMBL
Match: A0A2Z6NZV1 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_140560 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.4e-16
Identity = 63/206 (30.58%), Postives = 101/206 (49.03%), Query Frame = 0

Query: 689  IASSYFKDLFSSSNPTIVRESFSAIDAEVILNTPVGGEGTRDEIIWNREKKGLFTVKSAY 748
            + S    D     N  I+ + F+  +A  I++TP+      D+IIW+ E+ G+++V+ AY
Sbjct: 1295 LVSELIDDQTRQWNRAIIFKFFTNDEALKIVSTPLSLNLPADKIIWHWERDGVYSVRPAY 1354

Query: 749  HLAVLLSNSKMESPSTVTSHDTFWRKFWKIKALPKAKICVWRIIHDSLPTRVNILKKGIP 808
            HL     +  +  PS+ +  D+ W+K WK     K K  + R+  + LPTR N+ KKGI 
Sbjct: 1355 HLLCDARDINLPGPSS-SGDDSLWKKIWKAPIPNKVKNFMSRLAKNILPTRDNLKKKGIS 1414

Query: 809  INYLCPFSRSSEESAKHILWDCKLSKNLWKIFIPLTNGLFALNRGSWIPR--DYWAWLMD 868
            ++  CP   +  E+A H+   C +          L   LFA   G   P   D   WL++
Sbjct: 1415 LDTSCPLCHNDSENAHHLFMHCNM----------LKLALFASPLGCHPPMNVDLNCWLLE 1474

Query: 869  NL-AEEEL--EIAITILWSIWEYRNK 890
             L   ++L  ++  TILW  W  RN+
Sbjct: 1475 WLNCSDKLGAQLFCTILWKFWFARNQ 1489

HSP 2 Score: 194.9 bits (494), Expect = 1.8e-45
Identity = 284/1244 (22.83%), Postives = 464/1244 (37.30%), Query Frame = 0

Query: 73   IEDEDLEKATEDLQGAIFCRILTPKLINPEVFKTFMPRIWNKEGRVRIKAMGRNTYLCNF 132
            I D  +++   D    +  R LT + +N    K  M  +W  E  + +K +G   Y+  F
Sbjct: 29   IGDSSIQQPAYDF--CLVGRFLTERPVNFVAMKNTMASLWRPEEGMVVKEVGAGLYIFQF 88

Query: 133  NNSFEKDRIKRGGPWNYDRALLVIEDTQGASRISHTSFRYVNFWVHIHDLPMVCMRRKWA 192
                E +R+    PW+++   L++E    +   S  S  ++  WV ++ L       + A
Sbjct: 89   GALAEMERVMEMCPWSFNNQALLLERLGRSKDPSEVSLHHLYVWVRVYGLKRGFFSERVA 148

Query: 193  EKLGNSLGEFVEAD-LDEGGSTENTLRIQVKIDVSEPLKRGLMVRIGSKAEETWVKVTYE 252
            ++LGN +G+FVEAD  +      + LRI+VK+DV +PLK+G  ++   K E   V   YE
Sbjct: 149  QRLGNEIGQFVEADPKNFSNPWSSYLRIRVKLDVQKPLKKGTRLKREGK-EWFHVDFAYE 208

Query: 253  RLPEFCYGCGIIGHVQQEC-EKISNEGE--ENLYGDFMRATPIVGGTPKQKTQENKRGNF 312
            RLP FC+ CG +GH ++ C E +   G   E  +G  +RA+         +   N  G  
Sbjct: 209  RLPTFCFVCGRLGHGERFCPETLRQWGRQVERKFGPELRAS--------TRRASNNIGAR 268

Query: 313  WGRERGRRGAYQFQNNRQNQKYNDEKEETWRRRDQSGKSNEENVGVLRPENSTGGRSSPE 372
            W RE             +     DE ++    +++ GK +       R   +  G SS  
Sbjct: 269  WLRE-------------ELPPRGDEAQQ----QEKIGKGDSR-----RSTPAYTGLSS-- 328

Query: 373  KETVGKSQGQPTASEVSEQSRMKTGKAVMETNCQAVEIGKIRDLGQKYVPDKKATKGIVL 432
               + K  G P ++E++   R +    +ME++        +++   + + DK+    ++L
Sbjct: 329  --VLCKQDGVPPSTELAVWQRNREATHMMESS-------NLQNRFDQNLMDKQPDVEMLL 388

Query: 433  --------KDQTSEMITRKEWASGTEKDKKGP----RAKNSQSGLEKEI-NKALEREKHL 492
                    K    E       + G     K P        S+  ++ E+ + AL R +H 
Sbjct: 389  IPTDPKKRKKSVDEKNGESSGSGGLALLWKSPLNVTLLSLSRFHIDVEVEDLALGRWRHT 448

Query: 493  ------EEKEISE-----MDTNQDSLM-----------FRGEGKNVRTWKRRARVE--NN 552
                  ++   SE      D +Q S +                K     K R  ++    
Sbjct: 449  GFYGHPDQSRRSESWDLLRDLSQASTLPWICGGDFNAIMEQHEKQGGPPKPRYLIQAFRE 508

Query: 553  SVDSESLKGATIANVGRKKPLKRKRRSKKFEEAWIRVSDSKKIVESSWKEIPGRSFTDYS 612
            +V+   L    +      KP   + R   FE AW      + ++   W+     S  +  
Sbjct: 509  AVEDAGLMDFPLGGAWVPKPYAHRFR---FENAWRSDPTLRPLLLDCWEVSHAASLEE-- 568

Query: 613  NKLNRCLSNLLKWN---KDRIGGSIRAAIDRKRDEILRMEGDDSTANLASIGLAEKELEN 672
             KLN C + L  W    KD+    I      +R    R  G     +      A+K L  
Sbjct: 569  -KLNFCSTRLHVWGMEWKDQFASEINIMRTIQR----RTRGRRDHQSRIQFQRAKKRLFE 628

Query: 673  LLNEEESYWKLRSREDWLQWGDRNTKWFHHKASERKKRNEIKGLLNSSG---VWIDD-ED 732
            L   +E++W+ ++++ WL  GDRNTK+FH +ASER++ N I  L +S+G    W +  ED
Sbjct: 629  LYALKEAFWRQQAKQFWLTQGDRNTKFFHAQASERQRLNRIDRLTDSNGQLRTWSNGLED 688

Query: 733  EI--------------GNIASS-------------------------------------- 792
             I              G+I  S                                      
Sbjct: 689  TIKEYFEELYHAQGNPGHIIQSLIPSLVSREDNAMLRQPYSYEEVRQAVFSMHPDKSPGG 748

Query: 793  ---------------------------YFKD----------------------------- 852
                                       YF+D                             
Sbjct: 749  DGFNPGKMAWKFLTQPDLLVSKVFKAKYFRDCSFLEAQIGSNSSFVWKSIFATKDLLHTG 808

Query: 853  ------------------LFSSSNPTIVRES--------------------------FSA 912
                              L  + NP I  E+                          F+A
Sbjct: 809  IRWKVGPGTNISIWEDPWLMDNENPFITTENPGSIALTRVSDLRSPNGWDWPTIDAIFNA 868

Query: 913  IDAEVILNTPVGGEGTRDEIIWNREKKGLFTVKSAYHLAVLLSNSKMESPSTVTSHD--- 972
             D + I   P+ G  + D +IW  +K G++TVKSAY          +   +TV  H    
Sbjct: 869  RDRQCIEAIPLTGSSSNDMLIWAHDKSGIYTVKSAY--------KALTWDATVLGHGMDR 928

Query: 973  TFWRKFWKIKALPKAKICVWRIIHDSLPTRVNILKKGIPINYLCPFSRSSEESAKHILWD 1032
              W+K W ++ LPK +  +WR  ++ LP   N++ K + +  +CP   +SEE+  HI   
Sbjct: 929  ALWKKLWAVRVLPKVRNLIWRAANNILPCLNNLVTKRVTVQDVCPLCHTSEETVLHIFVH 988

Query: 1033 CKLSKNLWKIFIPLTNGLFALNRGSWIPRDYWAWLMDNLAEEELEIAITILWSIWEYRNK 1092
            C  ++ +W        G +A   GS+   D+   ++    E +  +A  +LW IW  RN 
Sbjct: 989  CPFARQVWGASF---LGWYAPAVGSF--HDWLLAVLHLFNEYDKGLAFHLLWMIWSARNA 1048

Query: 1093 VTHTESKPIYQEISRIISSKIDFPKVVSRTYLPKSSGKNQPTVKNLASHAIWKPPPDLSL 1113
                                    K V R+ L                   W+ PP   L
Sbjct: 1049 KVW---------------------KGVRRSPLEG-----------------WERPPVNYL 1108

BLAST of Spg016126 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 1.1e-34
Identity = 107/400 (26.75%), Postives = 188/400 (47.00%), Query Frame = 0

Query: 705  IVRESFSAIDAEVILNTPVGGEGTRDEIIWNREKKGLFTVKSAYHLAVLLSNSKMESPST 764
            ++   F  ++ ++I     GG    D   W+    G +TVKS Y +   + N K  SP  
Sbjct: 186  VIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIIN-KRSSPQE 245

Query: 765  VT--SHDTFWRKFWKIKALPKAKICVWRIIHDSLPTRVNILKKGIPINYLCPFSRSSEES 824
            V+  S +  ++K WK +  PK +  +W+ + +SLP    +  + +     C    S +E+
Sbjct: 246  VSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSCKET 305

Query: 825  AKHILWDCKLSKNLWKI-FIPLTNGLFALNRGSWIP----RDYWAWLMDN---LAEEELE 884
              H+L+ C  ++  W I  IP+  G      G W        YW + + N     E+  +
Sbjct: 306  VNHLLFKCTFARLTWAISSIPIPLG------GEWADSIYVNLYWVFNLGNGNPQWEKASQ 365

Query: 885  IAITILWSIWEYRNKVTHTESKPIYQEISRIISSKIDFPKVVSRTYLPKSSGKNQPTVKN 944
            +   +LW +W+ RN++     +   QE+ R     ++  ++  RT       K Q    N
Sbjct: 366  LVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRI--RTEAESCGTKPQ---VN 425

Query: 945  LASHAIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICVGFKRMHNRWSIKAL 1004
             +S   W+PPP   +K N DA+WN    + G+GW+LR+  G    +G + +         
Sbjct: 426  RSSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALP-------- 485

Query: 1005 EAKAVLEGLISILSVADLHPSLSSISLLV-ESDASSVVNLLNEEDEDFTEISFLIQEISR 1064
            + K+VLE  +  +  A L  S    + ++ ESD+  ++ +LN  DE +  +   IQ++ R
Sbjct: 486  KLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEILN-NDEIWPSLKPTIQDLQR 545

Query: 1065 LKKNFKEISFLYCPRDQNVAADLLARVAISFPSLVPVLDS 1094
            L   F E+ F++ PR+ N  A+ +AR ++SF +  P L S
Sbjct: 546  LLSQFTEVKFVFIPREGNTLAERVARESLSFLNYDPKLYS 564

BLAST of Spg016126 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 1.8e-21
Identity = 81/317 (25.55%), Postives = 145/317 (45.74%), Query Frame = 0

Query: 774  KFWKIKALPKAKICVWRIIHDSLPTRVNILKKGIPINYLCPFSRSSEESAKHILWDCKLS 833
            K WK+K  PK K  +W+++  +L T  N+ ++ I  +  C      +E+++H+ +DC  +
Sbjct: 17   KIWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFYA 76

Query: 834  KNLWKIF-IP----LTNGLFALNRGSWIPRDYWAWLMDNLAEEELEIAITILWSIWEYRN 893
            + +W+   IP     T G+    +   +     A    N   +   +AI ILW +W+ RN
Sbjct: 77   QQVWRASGIPHQELRTTGITMETKMELLLSSCLA----NRQPQLFNLAIWILWRLWKSRN 136

Query: 894  KVTHTESKPIYQEISRIISSKID-----FPKVVSRTYLPKSSGKNQPTVKNLASHAIWKP 953
            ++   +    +Q   +   + +         V S      SS   QPT+    +   W+ 
Sbjct: 137  QLVFQQKSISWQNTLQRARNDVQEWEDTNTYVQSLNQQVHSSRHQQPTM----ARTKWQR 196

Query: 954  PPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICVGFKRMHNRWSIKALEAKAVLEGL 1013
            PP   +K N D ++N Q R    GW++RD +G  + +G  +     +  +LE++      
Sbjct: 197  PPSTWIKYNYDGAFNHQTRNAKAGWLMRDENG--VYMGSGQAIGSTTSDSLESE------ 256

Query: 1014 ISILSVADLHP-SLSSISLLVESDASSVVNLLNEEDEDFTEISFLIQEISRLKKNFKEIS 1073
               L +A  H  S     ++ E D+  V  L+N E  +F   ++ I+E    +K F+E  
Sbjct: 257  FQALIIAMQHAWSQGYRKVIFEGDSKQVEELMNNEKLNFGRFNW-IREGRFWQKRFEEAV 316

Query: 1074 FLYCPRDQNVAADLLAR 1080
            F + PR  N  AD+LA+
Sbjct: 317  FKWVPRTNNQPADILAK 316

BLAST of Spg016126 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 2.0e-20
Identity = 88/292 (30.14%), Postives = 143/292 (48.97%), Query Frame = 0

Query: 813  CPFSRSSEESAKHILWDCKLSKNLWKIFIPLTNGLFALNRGSWIPRDY--WAWLMDNLAE 872
            CP SR   E+  H+L+ C  ++ +W I     + + A   G W    Y    W+++   E
Sbjct: 15   CPDSR---ETVNHLLFKCCFARLVWAI-----SPIPAYPEGEWTDSLYANLYWVLN--LE 74

Query: 873  EEL-------EIAITILWSIWEYRNKVTHTESKPIYQEISRIISSKIDFPKVVSRTYLP- 932
             E+        +   +LW +W+ RN++     +    E+ R   +  DF +  +R  L  
Sbjct: 75   VEIPKLGKIGNLVPWLLWRLWKSRNELMFKGKEYDAPEVLR--RAMEDFEEWSTRRELEG 134

Query: 933  KSSGKNQPTVKNLASHAIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICVGF 992
            K+SG   P V+   S   WK PP   +K N DA+W  +  + G+GWILR+ SG  + +G 
Sbjct: 135  KASG---PQVERNLS-VQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGA 194

Query: 993  KRMHNRWSIKALEAKAVLEGLISILSVADLHPSLSSIS-LLVESDASSVVNLLNEEDEDF 1052
            + +           K VLE  +  L  A L  S  +   ++ ESDA ++VNLLN  D+ +
Sbjct: 195  RALP--------RTKNVLEAELEALRWAVLTMSRFNYKRIIFESDAQALVNLLN-SDDFW 254

Query: 1053 TEISFLIQEISRLKKNFKEISFLYCPRDQNVAADLLARVAISFPSLVPVLDS 1094
              +   +++I +L  +F+E+ F + PR  N  AD +AR +ISF +  P L S
Sbjct: 255  PTLQPALEDIQQLLHHFEEVKFEFTPRGGNKVADRIARESISFSNYDPKLFS 281

BLAST of Spg016126 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 4.6e-09
Identity = 45/204 (22.06%), Postives = 89/204 (43.63%), Query Frame = 0

Query: 879  ILWSIWEYRNKVTHTESKPIYQEISRIISSKIDFPKVVSRTYLPKSSGKNQPTVKNLASH 938
            ++W IW+  N +    ++  +Q  + +  +  D  + +  T   +    N+    + + +
Sbjct: 47   LMWRIWKSGNDLVFNHTRTKFQ--TTVEMALNDTKEWLDNTMTNEQQNGNRNA--DPSRN 106

Query: 939  AIWKPPPDLSLKLNVDASWNDQFRKGGVGWILRDSSGSSICVGFKRMHNRWSIKALEAKA 998
              W PP    LK N DAS +++    G+GWILR+S G+ I  G  +   R + +  E   
Sbjct: 107  TKWSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECST 166

Query: 999  VLEGLISILSVADLHPSLSSISLLVESDASSVVNLLNEEDEDFTEISFLIQEISRLKKNF 1058
            ++  +                 ++ E D  ++  ++N +  +   +   +  I     +F
Sbjct: 167  LIWAI-------QASYGFGHKKVIFEGDNQTITRMINTKSSN-PRLQHFLDTIQSWIPSF 226

Query: 1059 KEISFLYCPRDQNVAADLLARVAI 1083
            + I F +  R+QN  AD LA+ AI
Sbjct: 227  ESIEFSFKHREQNGCADFLAKQAI 238

BLAST of Spg016126 vs. TAIR 10
Match: AT3G42140.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 55.8 bits (133), Expect = 2.5e-07
Identity = 34/137 (24.82%), Postives = 56/137 (40.88%), Query Frame = 0

Query: 141 IKRGGPWNYDRALLVIEDTQGASRISHTSFRYVNFWVHIHDLPMVCMRRKWAEKLGNSLG 200
           I R GPW+++  + VI+  +     S   F+ + FW+ I  +P+  +  +    +G  +G
Sbjct: 75  ILRRGPWSFNDWMCVIQ--RWTKLHSDAEFKRIPFWIQIRGIPLRFLTARIITSIGERMG 134

Query: 201 EFVEADLDEGGSTENTLRIQVKIDVSEPLKRGLMVRIGSKAEETWVKVTYERLPEFCYGC 260
            F+E +L                DVS                   +K  YE+L  FC  C
Sbjct: 135 LFLETNLGR--------------DVSV------------------LKFQYEKLKNFCTTC 177

Query: 261 GIIGHVQQECEKISNEG 278
           G++ H   EC    N+G
Sbjct: 195 GMLSHDASECPTSGNQG 177