Spg016114 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg016114
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAminoacyl-tRNA hydrolase
Locationscaffold9: 41411962 .. 41414903 (+)
RNA-Seq ExpressionSpg016114
SyntenySpg016114
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTTTTTTCCAGTCGAGCTTCAAGATTTCAGGCAAAAACACCCACAAGATAAGATTGAAATTGAAACAGCCGCGCTTTGCCCACTCTCTGTTTTTCTAAAAAGCTCATGTTGGGTTCTTCTAATTCTTCCAGAAAGGTATTCAATTTCATGGGTTGCTTTCTCTCTCCCTCTGTCCCTCTTCTCGATGATGTTAAAGCTTCATGGAAAATTGTTAACGTCGTGGATGTTGTTTGAATTAATTTATCGTTTTCTTTTCTGTGTTGTTAGAAGAAGCAAGTGAAGCCAGAAAAAGAATGGCTCTCAGTGAGTTTCAGACCCGAGAACTTCATACCAGGCCTCGTCATTGGTTTCATTCTTGGGTTGTTTTTGGATTTGTCAAAACCCAGTAAAGGCAATGTTAAGAAGGGTTATTTCCTGCCGTCCAAGTACCAACAGCTATCTCCAACCTCAAACGACGGCGATCAAGAGCTCAAATTGGTTAGTGTTCGTCTTTACCGGCTGGTTTATTTTAATTTTGCAGTTTGGTTTATTGTGACCTTTATCTGGAATTCTGTGGAGTTGGAAATGGTTTCTGTTCGTGATTGTGATGGATTATGATCACTAAAATAGAATGAAAGGGAAGAATGAAACGAGAATTTTTTAGTTTAGCCTCAACTGTGTTCGATAAAAGGGCATACAATAGTACTAGGGCTTTTAAAAATTAGCTTTCATATTGGGATGAGGTTTTCTGTTTAATGGCAATGAAAATGGTTCTCAATTGCTCAAATTTTGAGGCTTGAATTGCTTGTAAGACAGAAGAGTTTAGGGCCATGCTTGAGGTAGTTGTGTGATAATATTGGGAACAAAATTAGCTTTTGAGTGAACATGCCTATTTGCCACAACATTAAGTGATGAAGCAGAGTTTAGGTACGTTGCTTACACCATCTTTGCTCTTGCCCATCAAGTATCTACAGTCCGTCCTGCCAAGACGTGAAAAGGGAACCGATTAGTTGAGGATTAAAAGGGCCTACTGAAATATGATATATCAGTTTAGACGAGTAATCTTTGCTTTGCAACACTTAGTTATATGTATATTTCCAAATATGTTAAAATGTTGTATTATGGCTCGTTAGATTTTCCCCCATTAAGCGTTGAAGCACACTAGCAGTAACTGCCACACGAAGTCCTTGCATCGTGTAACTCGTATAATGTGTTTAGCTGTCGAGAAACATCTGACATTCTGACTTTTAATTGAAGGTCCTGGTTGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCGTAAGATTTAAGATGCTGCATTTCATATTTGTCATAGTGTGCATTTAATTCTAGTTTGATTGCTTTGGTCCATGTATCACTTCTCATGTCTTCCCGCCATCAATGCCAAATGTTTTTGCATGTTTTCTGTCAAATTATGAAGATGCTGCTACTGGCTTGTATGCAGAACTAATGCAAAGGTACTCCTTATACTATACTACTTCCGAATATCTGATTTGATCAAAGCATGTACTCAGCTAATTCTTCACCCGTCTCTCTTTTCTGCATCTATATCCATTTCTAATATCTAGTGCAGCCACCGAAACCTTTTGAGGCAGTGGGAGCTTTGTGGGCAGCCAAAAATAGTTGTAACATGCAAGAATCAACAAGAAATGTAAGCAGGTGATTCCTTTTTCTGAAAATGTAATCATCTAAGTGATAAACATGTGAAATCTTATGGTTTTTGAAAGACTTGTTGGCTTTTGTTGAGGATAGGGAGGAGGGGAAGAGAACGGTTGGGAAGGATTGTTTATTGAAGATACACTTTTGTTGTTAAATTTATTTCTTTTCTCAAATCAGGAACAAACTGAAGGATGCAGCTGAGACCATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTACAATCTTTGTCCTGTACTTACAAGCATTTTGATTAATGCATGAGAAATGATCGGGTAACCAGGGACGATAAATGCTTAACATGTTGAATTACTAATGTTTATGTTTTGGTACACGTAGCCGAATGATTTTTCACATGTTATGCGGGTCGGTATTATATTGTCCTATGAATGTGATGCATCAGCAAAATATGTTCAGCTCTCTGGAACCTGGTATCCTGAAATTGGTTCAATGATATGCAGGTTTCGGCTGGATCAAAGACCGTTCTTGCCATTGGACCCGGTAATTCTTCTTTACCCTGTGATAGAATTCTGTGGTTGCCTGGTTGATTTTCCTTATGTCACTCGTCATTCTTGTGTATATACCCTTGCACTCATGAGTTTGGAAATTAGCACTTGATCCAATGAATGATTCTCTCGATATAAATTGGAGATGAAATGACATTACTGGCATTTAAACACAACCTCTTGCTCTCACTGAATCTGAAAACTTAAGCGGATGAGTTATGGTAAATTTAATTATTTATTCATATTCTTAACAATATGCACGTTCCTCGACTCTTTTTTAAATAGGGCCAAAGGAGTCTGTTGATGCGGTAACGGGTAAACTTCGCCTACTCTAGTCGGGTTGGAATCAGGACAGACAGTCATACGCTTGATTTGATGCATTCAAGACGAAAATGATGAATCTGCGTCTTTCTCCTTTAGCATTCAATTTGTTGAGGTTACAAGAGGGGTTCGTGCACATGGTGGATAAGTTATAACGGCTGGTAAGAGAGAAGTGTTGTTTGTGGAGTATGGTTATGTCTGCAACATTGTTTAGTATTGTTATTAGTTGCTCTTAGTATGAGTGACATTTGAACTGACATGTTTAGGATGTAACCTTCGATCTTGAAGAATGTTTGTGACTTATTGACGTTTGTTTGGCCAAACAAATTTTTAAAACATTTGTACGGAGCTGTAGTTGGATGGCTGGTAAATTTTGGAGATTAATATGTTTTTTGTTCTCGAG

mRNA sequence

ATGTTGGGTTCTTCTAATTCTTCCAGAAAGAAGAAGCAAGTGAAGCCAGAAAAAGAATGGCTCTCAGTGAGTTTCAGACCCGAGAACTTCATACCAGGCCTCGTCATTGGTTTCATTCTTGGGTTGTTTTTGGATTTGTCAAAACCCAGTAAAGGCAATGTTAAGAAGGGTTATTTCCTGCCGTCCAAGTACCAACAGCTATCTCCAACCTCAAACGACGGCGATCAAGAGCTCAAATTGGTCCTGGTTGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCATGCTGCTACTGGCTTGTATGCAGAACTAATGCAAAGGAACAAACTGAAGGATGCAGCTGAGACCATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTTTCGGCTGGATCAAAGACCGTTCTTGCCATTGGACCCGGGCCAAAGGAGTCTGTTGATGCGGTAACGGGTAAACTTCGCCTACTCTAG

Coding sequence (CDS)

ATGTTGGGTTCTTCTAATTCTTCCAGAAAGAAGAAGCAAGTGAAGCCAGAAAAAGAATGGCTCTCAGTGAGTTTCAGACCCGAGAACTTCATACCAGGCCTCGTCATTGGTTTCATTCTTGGGTTGTTTTTGGATTTGTCAAAACCCAGTAAAGGCAATGTTAAGAAGGGTTATTTCCTGCCGTCCAAGTACCAACAGCTATCTCCAACCTCAAACGACGGCGATCAAGAGCTCAAATTGGTCCTGGTTGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCATGCTGCTACTGGCTTGTATGCAGAACTAATGCAAAGGAACAAACTGAAGGATGCAGCTGAGACCATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTTTCGGCTGGATCAAAGACCGTTCTTGCCATTGGACCCGGGCCAAAGGAGTCTGTTGATGCGGTAACGGGTAAACTTCGCCTACTCTAG

Protein sequence

MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFLPSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKESVDAVTGKLRLL
Homology
[Close]
BLAST of Spg016114 vs. NCBI nr
Match: XP_008466065.1 (PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 292.7 bits (748), Expect = 1.9e-75
Identity = 155/192 (80.73%), Postives = 161/192 (83.85%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGN KKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAAE IGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. NCBI nr
Match: XP_022157533.1 (peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 290.4 bits (742), Expect = 9.7e-75
Identity = 155/192 (80.73%), Postives = 159/192 (82.81%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           ML SSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI GLFLDLSKPSKGN K+GYFL
Sbjct: 1   MLKSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIFGLFLDLSKPSKGNAKRGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQL+P SNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLTPASNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAA+ IGLPTFVVADAGRTQVSAGSKTVLAIGPGPK 
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAADNIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKA 180

[Close]
BLAST of Spg016114 vs. NCBI nr
Match: XP_004136220.1 (peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis sativus] >KGN60321.1 hypothetical protein Csa_002634 [Cucumis sativus])

HSP 1 Score: 290.0 bits (741), Expect = 1.3e-74
Identity = 153/192 (79.69%), Postives = 161/192 (83.85%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGN KKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKD+AE IGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. NCBI nr
Match: XP_022941498.1 (peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita moschata] >XP_022981057.1 peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita maxima] >XP_023523204.1 peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >KAG6608093.1 Peptidyl-tRNA hydrolase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 288.1 bits (736), Expect = 4.8e-74
Identity = 152/192 (79.17%), Postives = 158/192 (82.29%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLGSSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI G  LDLSKPSKGN KKGYFL
Sbjct: 1   MLGSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIAGFLLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQ+SP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYA+LMQ        
Sbjct: 61  PSKYQQISPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAQLMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAAE +GLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENVGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. NCBI nr
Match: XP_038900235.1 (peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 287.3 bits (734), Expect = 8.2e-74
Identity = 155/192 (80.73%), Postives = 161/192 (83.85%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLGSSNSSRKK+Q+K EKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPS+GN KKGYFL
Sbjct: 1   MLGSSNSSRKKQQLKSEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSRGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSP-SNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAAE IGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. ExPASy Swiss-Prot
Match: Q9Y3E5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.0e-18
Identity = 60/161 (37.27%), Postives = 83/161 (51.55%), Query Frame = 0 

Query: 33  GLVIGFILGLFLDLS-KPSKGNVKKGYFLPSKYQQLSPTSNDGDQ-ELKLVLVVRQDLKM 92
           GL +G   G+ L  S +   G + K     +     S  S  GD  E K++LVVR DLKM
Sbjct: 18  GLAVGVACGMCLGWSLRVCFGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKM 77

Query: 93  GSGKIASQCAHAATGLYAELMQRN--------------------------KLKDAAETIG 152
           G GK+A+QC+HAA   Y ++ +RN                           L   A+ +G
Sbjct: 78  GKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLG 137

Query: 153 LPTFVVADAGRTQVSAGSKTVLAIGPGPKESVDAVTGKLRL 166
           L   ++ DAGRTQ++ GS+TVL IGPGP + +D VTG L+L
Sbjct: 138 LTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKL 178

[Close]
BLAST of Spg016114 vs. ExPASy Swiss-Prot
Match: Q3ZBL5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.6e-18
Identity = 48/115 (41.74%), Postives = 67/115 (58.26%), Query Frame = 0 

Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN---------------------- 136
           E K++LVVR DLKMG GK+A+QC+HAA   Y ++ +RN                      
Sbjct: 64  EYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPELLKEWEYCGQPKVVVKAPDE 123

Query: 137 ----KLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKESVDAVTGKLRL 166
               +L   A+ +GL   ++ DAGRTQ++ GS+TVL IGPGP + +D VTG L+L
Sbjct: 124 ETLVELLTHAKVLGLTVSLIQDAGRTQIAPGSRTVLGIGPGPADLIDKVTGHLKL 178

[Close]
BLAST of Spg016114 vs. ExPASy Swiss-Prot
Match: Q8R2Y8 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.9e-17
Identity = 49/115 (42.61%), Postives = 66/115 (57.39%), Query Frame = 0 

Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN---------------------- 136
           E K++LVVR DLKMG GK+A+QC+HAA   Y +  +R+                      
Sbjct: 66  EYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDE 125

Query: 137 ----KLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKESVDAVTGKLRL 166
               +L   A+T+GL   ++ DAGRTQ+  GS+TVL IGPGP E +D VTG L+L
Sbjct: 126 DTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKL 180

[Close]
BLAST of Spg016114 vs. ExPASy Swiss-Prot
Match: O97067 (Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-15
Identity = 60/179 (33.52%), Postives = 82/179 (45.81%), Query Frame = 0 

Query: 27  PENFIPGL--VIGFILGLFLDLSK-PSKGNVKKGYFLPSKYQQLSPTSNDG--------- 86
           P   I GL  ++ F +G    L +  +K +V +G   P   +    + ++          
Sbjct: 8   PTQIINGLAVMLSFFVGYRYALKRGDAKDSVTEGAATPFSQESSVSSGSEASVSDKGYGG 67

Query: 87  -DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN------------------- 146
            +   K+VLVVR DLKMG GKIA+QC H A G Y   + R                    
Sbjct: 68  LNDNFKMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRV 127

Query: 147 -------KLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKESVDAVTGKLRLL 167
                   +K  AE   L T ++ DAGRTQ+ A SKTVLA+GP     +D VTG L+LL
Sbjct: 128 ESEAELMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAVGPAAAADIDRVTGHLKLL 186

[Close]
BLAST of Spg016114 vs. ExPASy Swiss-Prot
Match: O13830 (Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC19A8.14 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-15
Identity = 66/195 (33.85%), Postives = 90/195 (46.15%), Query Frame = 0 

Query: 23  VSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFLPSKYQQLSP--TSNDGDQE--- 82
           +S  P   + G V+GF++G    ++  S+G   K     S  ++ SP   SND D E   
Sbjct: 10  ISSFPAAVLVGAVVGFMIGRKYSVADASRGYSSKNANKASNPEKESPVSVSNDEDSESET 69

Query: 83  ---------------------LKLVLVVRQDLKMGSGKIASQCAHAATGLY--------- 142
                                 K+VLVVR DL M  GKIA+QCAHAA   Y         
Sbjct: 70  ELLDMLKGNSSLAALALAEGQTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPD 129

Query: 143 ----------------AELMQRNKLKDA-AETIGLPTFVVADAGRTQVSAGSKTVLAIGP 166
                           A+  +  +L  A A ++GL   V+ DAGRTQ+++GS TVL IGP
Sbjct: 130 LVRIWENAGQAKITLQAQTEETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGP 189

[Close]
BLAST of Spg016114 vs. ExPASy TrEMBL
Match: A0A1S3CRS9 (Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 9.4e-76
Identity = 155/192 (80.73%), Postives = 161/192 (83.85%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGN KKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAAE IGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. ExPASy TrEMBL
Match: A0A6J1DTL2 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111024134 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.7e-75
Identity = 155/192 (80.73%), Postives = 159/192 (82.81%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           ML SSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI GLFLDLSKPSKGN K+GYFL
Sbjct: 1   MLKSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIFGLFLDLSKPSKGNAKRGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQL+P SNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLTPASNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAA+ IGLPTFVVADAGRTQVSAGSKTVLAIGPGPK 
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAADNIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKA 180

[Close]
BLAST of Spg016114 vs. ExPASy TrEMBL
Match: A0A0A0LJW4 (Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G895670 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 6.1e-75
Identity = 153/192 (79.69%), Postives = 161/192 (83.85%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLG+S SSRKK+QVKPEKEWLSVSF+PENFIPGLVIGFILGLFLDLSKPSKGN KKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQLSP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ        
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKD+AE IGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. ExPASy TrEMBL
Match: A0A6J1ISX6 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111480317 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.3e-74
Identity = 152/192 (79.17%), Postives = 158/192 (82.29%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLGSSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI G  LDLSKPSKGN KKGYFL
Sbjct: 1   MLGSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIAGFLLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQ+SP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYA+LMQ        
Sbjct: 61  PSKYQQISPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAQLMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAAE +GLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENVGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. ExPASy TrEMBL
Match: A0A6J1FS99 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111446781 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 2.3e-74
Identity = 152/192 (79.17%), Postives = 158/192 (82.29%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           MLGSSNSSRKK+QVKPEKEWLSVSFRPENFIPGLVIGFI G  LDLSKPSKGN KKGYFL
Sbjct: 1   MLGSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIAGFLLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ-------- 120
           PSKYQQ+SP SN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYA+LMQ        
Sbjct: 61  PSKYQQISPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAQLMQSHRNLLRQ 120

Query: 121 ------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 167
                              NKLKDAAE +GLPTFVVADAGRTQVSAGSKTVLAIGPGPKE
Sbjct: 121 WELCGQPKIVVTCKNQQEMNKLKDAAENVGLPTFVVADAGRTQVSAGSKTVLAIGPGPKE 180

[Close]
BLAST of Spg016114 vs. TAIR 10
Match: AT4G32900.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 192.2 bits (487), Expect = 3.3e-49
Identity = 107/193 (55.44%), Postives = 135/193 (69.95%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           M  SS +S + + ++ EK+ +SVSFR EN IPG+VIGFI+G+ LDLS+     VK+   L
Sbjct: 1   MWPSSRNSSQPQAMRQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLL 60

Query: 61  PSKYQQLSPTSNDG-DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQ------- 120
            SK Q+ S    +G DQELK+VLVVRQDLKM +GKIASQCAHAATG+YAELMQ       
Sbjct: 61  SSKVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLR 120

Query: 121 -------------------RNKLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPK 167
                               NK+ +AAE++GLPTFVVADAGRT+V+AGS+TVLA+GPGPK
Sbjct: 121 RWEENGQPKIVVTCKNQQEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLAVGPGPK 180

[Close]
BLAST of Spg016114 vs. TAIR 10
Match: AT4G32900.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 128.3 bits (321), Expect = 5.9e-30
Identity = 74/132 (56.06%), Postives = 93/132 (70.45%), Query Frame = 0 

Query: 1   MLGSSNSSRKKKQVKPEKEWLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFL 60
           M  SS +S + + ++ EK+ +SVSFR EN IPG+VIGFI+G+ LDLS+     VK+   L
Sbjct: 1   MWPSSRNSSQPQAMRQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLL 60

Query: 61  PSKYQQLSPTSNDG-DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRNK-LKD 120
            SK Q+ S    +G DQELK+VLVVRQDLKM +GKIASQCAHAATG+YAELMQ ++ L  
Sbjct: 61  SSKVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLR 120

Query: 121 AAETIGLPTFVV 131
             E  G P  VV
Sbjct: 121 RWEENGQPKIVV 132

[Close]
BLAST of Spg016114 vs. TAIR 10
Match: AT3G03010.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 90.5 bits (223), Expect = 1.4e-18
Identity = 58/143 (40.56%), Postives = 83/143 (58.04%), Query Frame = 0 

Query: 50  SKGNVKKGYFLPSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAE 109
           S GN K     P + ++L+    D  +  K+VLVVR DLKMG GKIA+QC+HA  GLY +
Sbjct: 42  SSGNKKTKSKEPLEIEKLA----DFRKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKK 101

Query: 110 LMQR-----NK---------------------LKDAAETIGLPTFVVADAGRTQVSAGSK 167
           L+QR     N+                     L++ A+T+ LPT +  DAG+TQ++  S+
Sbjct: 102 LLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSR 161

[Close]
BLAST of Spg016114 vs. TAIR 10
Match: AT3G03010.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 90.5 bits (223), Expect = 1.4e-18
Identity = 58/143 (40.56%), Postives = 83/143 (58.04%), Query Frame = 0 

Query: 50  SKGNVKKGYFLPSKYQQLSPTSNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAE 109
           S GN K     P + ++L+    D  +  K+VLVVR DLKMG GKIA+QC+HA  GLY +
Sbjct: 42  SSGNKKTKSKEPLEIEKLA----DFRKNFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKK 101

Query: 110 LMQR-----NK---------------------LKDAAETIGLPTFVVADAGRTQVSAGSK 167
           L+QR     N+                     L++ A+T+ LPT +  DAG+TQ++  S+
Sbjct: 102 LLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSR 161

[Close]
BLAST of Spg016114 vs. TAIR 10
Match: AT5G16870.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.0e-18
Identity = 66/174 (37.93%), Postives = 88/174 (50.57%), Query Frame = 0 

Query: 20  WLSVSFRPENFIPGLVIGFILGLFLDLSKPSKGNVKKGYFLPSKYQQLS-PTSNDGDQEL 79
           WLS        I G  +G+ +G     SKP    V K     S    L      D   + 
Sbjct: 5   WLSA------LIVGAALGYCIG--TRRSKPVVAAVDKDIKTQSSKSPLEIEKLADILDDF 64

Query: 80  KLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQRN------------------------ 139
           K+VLVVR DLKMG GKIA+QC+HA  GLY +L++R                         
Sbjct: 65  KMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLVRRAPKALDCWEECAQPKVVVKIEDEDE 124

Query: 140 --KLKDAAETIGLPTFVVADAGRTQVSAGSKTVLAIGPGPKESVDAVTGKLRLL 167
             +L++ A+++ LPT +  DAGRTQ++  S+TV+AI  GP E VD VTG L+LL
Sbjct: 125 MLELQERAKSLKLPTHITIDAGRTQIAPNSRTVMAI-LGPVEVVDEVTGGLKLL 169

The following BLAST results are available for this feature:
BLAST of Spg016114 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008466065.11.9e-7580.73PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis melo][more]
XP_022157533.19.7e-7580.73peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Momordica charantia][more]
XP_004136220.11.3e-7479.69peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis sativus] >KGN60321.... [more]
XP_022941498.14.8e-7479.17peptidyl-tRNA hydrolase 2, mitochondrial [Cucurbita moschata] >XP_022981057.1 pe... [more]
XP_038900235.18.2e-7480.73peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Benincasa hispida][more]
BLAST of Spg016114 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9Y3E53.0e-1837.27Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 S... [more]
Q3ZBL58.6e-1841.74Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=... [more]
Q8R2Y81.9e-1742.61Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 ... [more]
O970671.1e-1533.52Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 ... [more]
O138301.1e-1533.85Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
BLAST of Spg016114 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3CRS99.4e-7680.73Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1[more]
A0A6J1DTL24.7e-7580.73Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111024134 PE=4 SV=... [more]
A0A0A0LJW46.1e-7579.69Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G895670 PE=4 SV=1[more]
A0A6J1ISX62.3e-7479.17Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111480317 PE=4 SV=1[more]
A0A6J1FS992.3e-7479.17Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111446781 PE=4 SV=1[more]
BLAST of Spg016114 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G32900.23.3e-4955.44Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT4G32900.15.9e-3056.06Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT3G03010.11.4e-1840.56Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT3G03010.21.4e-1840.56Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
AT5G16870.13.0e-1837.93Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023476Peptidyl-tRNA hydrolase II domain superfamilyGENE3D3.40.1490.10Bit1coord: 73..113
e-value: 2.6E-14
score: 55.1
IPR023476Peptidyl-tRNA hydrolase II domain superfamilyGENE3D3.40.1490.10Bit1coord: 114..166
e-value: 3.4E-18
score: 67.6
IPR023476Peptidyl-tRNA hydrolase II domain superfamilySUPERFAMILY102462Peptidyl-tRNA hydrolase IIcoord: 77..166
IPR002833Peptidyl-tRNA hydrolase, PTH2PFAMPF01981PTH2coord: 115..166
e-value: 1.1E-15
score: 57.9
IPR002833Peptidyl-tRNA hydrolase, PTH2PANTHERPTHR12649PEPTIDYL-TRNA HYDROLASE 2coord: 114..166
IPR002833Peptidyl-tRNA hydrolase, PTH2PANTHERPTHR12649PEPTIDYL-TRNA HYDROLASE 2coord: 5..115
NoneNo IPR availablePANTHERPTHR12649:SF11PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIALcoord: 5..115
NoneNo IPR availablePANTHERPTHR12649:SF11PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIALcoord: 114..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg016114.1Spg016114.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity