Spg016068 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg016068
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Eukaryotic aspartyl protease family protein
Location	scaffold6: 44623258 .. 44624700 (-)
RNA-Seq Expression	Spg016068
Synteny	Spg016068

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTCCCCTGTTTTTCTCTTCCTCCTCTGTTTTCTCCTTCCTTCCTCTGTTTTCTCATCGGAGATTCTCCTTCTACCTCTCTCCCATTCCTTATCATTCTCTACAGATTTCAACAACACCCACAACCTCCTCAAATCCACCGCCGCCCGCTCCTCCGCCCGCTTCCACCACCGCCGCCATGCCCACCGCCGCAGCCACCTCTCTCTCCCCCTCTCCCCCGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCCGAAAATCACAAAATTTCCCTCTATATGGACACCGGCAGCGACCTTGTTTGGTTCCCCTGTTCCCCTTTTGAGTGTATTCTCTGTGAAGGCAAGCCAAAAATTCAATCCCCTTTGCCCAAAATCTCAAAACACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCCCACGGTGGCTCCCTCTCCTCCTCCCACCTCTGTGCAATTTCCCATTGCCCACTTGATTCCATTGAAATCTCTGAGTGCTCTTCCTTTTCTTGTCCGCCGTTTTATTATGCTTATGGCGATGGGAGTCTAATTGCTCAGCTTTACAGAGACAGTCTCAGTTTGCCGGCGCCGGCGACGGCACCGGCGATCAGTGTTCGGAATTTTACATTTGGGTGTGCCCACACTACCCTCGGCGAGCCGGTCGGGGTCGCCGGGTTCGGCCGGGGGACGTTGTCGATGCCGAGCCAACTCGCCACTTTCTCGCCCCAATTGGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCAGCAGAGCGGGTTCGTCGCCCGAGTCCGCTGATTCTCGGCCGCTACTACGGCGGCGAGACGGAGTTCGTTTACACTTCTTTGCTTGAGAATCCGAAGCATCCTTATTTTTACTCTGTTGGGTTGGCCGGAATTTCGGTGGGCACGGTGAGGATTCCGGCGCCGGAGTTTCTGAGAAGGGTGGATGAGGGCGGCAGCGGCGGGGTTGTGGTGGATTCCGGTACTACTTTCACTATGCTGCCGGCGGGTTTGTATGACTCGGTGGTGGCTCAGTTTGAGAACCGAACCGGTCGAGTTGCGTCGCGGGCGAGCCGGATTGAAGAAAATACCGGGTTGAGCCCTTGCTATTACTATGATGACTCAGTTGAAGTGCCACGTGTCGTGTTGCACTTCGTTGGGGAAAAATCCAGTGTGGTGCTGCCTAGGAAGAATTATTTTTACGAGTTTTTGGACGGTGGAGATGGAGTGGGGAGGAAGAGAAAAGTCGGGTGTTTGATGCTGATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCCGGAGCCACACTTGGGAACTATCAACAACAGGGTTTTGAGGTGCTTTATGATTTGGAGAAAAACCGGGTCGGGTTCGCCCGCCGGCAGTGTTCGACCCTTTGGGACAGTTTGAACCGGAATTAG

mRNA sequence

ATGACTTCCCCTGTTTTTCTCTTCCTCCTCTGTTTTCTCCTTCCTTCCTCTGTTTTCTCATCGGAGATTCTCCTTCTACCTCTCTCCCATTCCTTATCATTCTCTACAGATTTCAACAACACCCACAACCTCCTCAAATCCACCGCCGCCCGCTCCTCCGCCCGCTTCCACCACCGCCGCCATGCCCACCGCCGCAGCCACCTCTCTCTCCCCCTCTCCCCCGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCCGAAAATCACAAAATTTCCCTCTATATGGACACCGGCAGCGACCTTGTTTGGTTCCCCTGTTCCCCTTTTGAGTGTATTCTCTGTGAAGGCAAGCCAAAAATTCAATCCCCTTTGCCCAAAATCTCAAAACACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCCCACGGTGGCTCCCTCTCCTCCTCCCACCTCTGTGCAATTTCCCATTGCCCACTTGATTCCATTGAAATCTCTGAGTGCTCTTCCTTTTCTTGTCCGCCGTTTTATTATGCTTATGGCGATGGGAGTCTAATTGCTCAGCTTTACAGAGACAGTCTCAGTTTGCCGGCGCCGGCGACGGCACCGGCGATCAGTGTTCGGAATTTTACATTTGGGTGTGCCCACACTACCCTCGGCGAGCCGGTCGGGGTCGCCGGGTTCGGCCGGGGGACGTTGTCGATGCCGAGCCAACTCGCCACTTTCTCGCCCCAATTGGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCAGCAGAGCGGGTTCGTCGCCCGAGTCCGCTGATTCTCGGCCGCTACTACGGCGGCGAGACGGAGTTCGTTTACACTTCTTTGCTTGAGAATCCGAAGCATCCTTATTTTTACTCTGTTGGGTTGGCCGGAATTTCGGTGGGCACGGTGAGGATTCCGGCGCCGGAGTTTCTGAGAAGGGTGGATGAGGGCGGCAGCGGCGGGGTTGTGGTGGATTCCGGTACTACTTTCACTATGCTGCCGGCGGGTTTGTATGACTCGGTGGTGGCTCAGTTTGAGAACCGAACCGGTCGAGTTGCGTCGCGGGCGAGCCGGATTGAAGAAAATACCGGGTTGAGCCCTTGCTATTACTATGATGACTCAGTTGAAGTGCCACGTGTCGTGTTGCACTTCGTTGGGGAAAAATCCAGTGTGGTGCTGCCTAGGAAGAATTATTTTTACGAGTTTTTGGACGGTGGAGATGGAGTGGGGAGGAAGAGAAAAGTCGGGTGTTTGATGCTGATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCCGGAGCCACACTTGGGAACTATCAACAACAGGGTTTTGAGGTGCTTTATGATTTGGAGAAAAACCGGGTCGGGTTCGCCCGCCGGCAGTGTTCGACCCTTTGGGACAGTTTGAACCGGAATTAG

Coding sequence (CDS)

ATGACTTCCCCTGTTTTTCTCTTCCTCCTCTGTTTTCTCCTTCCTTCCTCTGTTTTCTCATCGGAGATTCTCCTTCTACCTCTCTCCCATTCCTTATCATTCTCTACAGATTTCAACAACACCCACAACCTCCTCAAATCCACCGCCGCCCGCTCCTCCGCCCGCTTCCACCACCGCCGCCATGCCCACCGCCGCAGCCACCTCTCTCTCCCCCTCTCCCCCGGCGGCGATTACACTCTCTCCTTCAACCTCGGCTCCGAAAATCACAAAATTTCCCTCTATATGGACACCGGCAGCGACCTTGTTTGGTTCCCCTGTTCCCCTTTTGAGTGTATTCTCTGTGAAGGCAAGCCAAAAATTCAATCCCCTTTGCCCAAAATCTCAAAACACAAATCAGTTTCCTGCAGCGCCGCCGCCTGCTCCGCCGCCCACGGTGGCTCCCTCTCCTCCTCCCACCTCTGTGCAATTTCCCATTGCCCACTTGATTCCATTGAAATCTCTGAGTGCTCTTCCTTTTCTTGTCCGCCGTTTTATTATGCTTATGGCGATGGGAGTCTAATTGCTCAGCTTTACAGAGACAGTCTCAGTTTGCCGGCGCCGGCGACGGCACCGGCGATCAGTGTTCGGAATTTTACATTTGGGTGTGCCCACACTACCCTCGGCGAGCCGGTCGGGGTCGCCGGGTTCGGCCGGGGGACGTTGTCGATGCCGAGCCAACTCGCCACTTTCTCGCCCCAATTGGGGAACCGGTTTTCTTATTGTTTGGTTTCTCACTCGTTTGCAGCAGAGCGGGTTCGTCGCCCGAGTCCGCTGATTCTCGGCCGCTACTACGGCGGCGAGACGGAGTTCGTTTACACTTCTTTGCTTGAGAATCCGAAGCATCCTTATTTTTACTCTGTTGGGTTGGCCGGAATTTCGGTGGGCACGGTGAGGATTCCGGCGCCGGAGTTTCTGAGAAGGGTGGATGAGGGCGGCAGCGGCGGGGTTGTGGTGGATTCCGGTACTACTTTCACTATGCTGCCGGCGGGTTTGTATGACTCGGTGGTGGCTCAGTTTGAGAACCGAACCGGTCGAGTTGCGTCGCGGGCGAGCCGGATTGAAGAAAATACCGGGTTGAGCCCTTGCTATTACTATGATGACTCAGTTGAAGTGCCACGTGTCGTGTTGCACTTCGTTGGGGAAAAATCCAGTGTGGTGCTGCCTAGGAAGAATTATTTTTACGAGTTTTTGGACGGTGGAGATGGAGTGGGGAGGAAGAGAAAAGTCGGGTGTTTGATGCTGATGAACGGTGGAGATGAGGCTGAGCTGGCAGGTGGGCCCGGAGCCACACTTGGGAACTATCAACAACAGGGTTTTGAGGTGCTTTATGATTTGGAGAAAAACCGGGTCGGGTTCGCCCGCCGGCAGTGTTCGACCCTTTGGGACAGTTTGAACCGGAATTAG

Protein sequence

MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRRHAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAYGDGSLIAQLYRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSVGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVASRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLNRN

Homology

[Close]

BLAST of Spg016068 vs. NCBI nr
Match: XP_023553227.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 892.1 bits (2304), Expect = 2.1e-255
Identity = 440/482 (91.29%), Postives = 461/482 (95.64%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRR 60
           M SPVFLFLLCFLL S VFSS++LLLPLS+SLS S+DFNNTHNLLKSTAARSSARFHHRR
Sbjct: 1   MASPVFLFLLCFLLSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  HAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120
             H RSHLSLPLSPGGDYTLSFNLGSE+ KISLYMDTGSDLVWFPCSPFECILCEGKPKI
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYA 180
           QSPLPKI+  KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIE+SECSSFSCPPFYYA
Sbjct: 121 QSPLPKIADKKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLSLPAPATA--PAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPS 240
           YGDGSLI +LYRDSLSLPAPA A  PAI+VRNFTFGCAH+ LGEP+GVAGFGRG LSMPS
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPS 240

Query: 241 QLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFY 300
           QLATFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYYG ETEF+YTSLLENPKHPYFY
Sbjct: 241 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSLLENPKHPYFY 300

Query: 301 SVGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGR 360
           SVGLAGISVG+VRIPAPEFL+RVDEGGSGGVVVDSGTTFTMLPAGLY+SVVAQFENRTGR
Sbjct: 301 SVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGR 360

Query: 361 VASRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGR 420
           VASRASRIEENTGLSPCYYY++SVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGV R
Sbjct: 361 VASRASRIEENTGLSPCYYYENSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVER 420

Query: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLN 480
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLN
Sbjct: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLN 480

[Close]

BLAST of Spg016068 vs. NCBI nr
Match: XP_023007805.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 891.7 bits (2303), Expect = 2.7e-255
Identity = 436/480 (90.83%), Postives = 459/480 (95.62%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRR 60
           M SPVFLFLLCFLLPS VFSS+ILLLPLS+SLS S+DFNNTHNLLKSTAARSSARFHHRR
Sbjct: 1   MASPVFLFLLCFLLPSPVFSSQILLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  HAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120
             H RSHLSLPLSPGGDYTLSFNLGSE+ KISLYMDTGSDLVWFPCSPFECILCEGKPKI
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYA 180
           QSPLPKIS  KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIE+SECSSFSCPPFYYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQL 240
           YGDGSLI +LYRDSLSLPAPA +PAI+VRNFTFGCAH+ LGEP+GVAGFGRG LSMP QL
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPIQL 240

Query: 241 ATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSV 300
           ATFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYYG ETEF+YTS+LENPKHPYFYSV
Sbjct: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFYSV 300

Query: 301 GLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVA 360
           GLAGISVG+V IPAPEFL++VDEGGSGGVVVDSGTTFTMLPAGLY+SVVAQFENRTGRVA
Sbjct: 301 GLAGISVGSVMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGRVA 360

Query: 361 SRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKR 420
           SRAS+IEENTGLSPCYYY+ SVEVPRVVLHFVGEKSSV+LPRKNYFYEFLDGGDGVGRK 
Sbjct: 361 SRASQIEENTGLSPCYYYEKSVEVPRVVLHFVGEKSSVMLPRKNYFYEFLDGGDGVGRKI 420

Query: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLNRN 480
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLNR+
Sbjct: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLNRS 480

[Close]

BLAST of Spg016068 vs. NCBI nr
Match: KAG6577689.1 (putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 889.0 bits (2296), Expect = 1.8e-254
Identity = 438/482 (90.87%), Postives = 459/482 (95.23%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRR 60
           M SPVFLFLLCFL+ S VFSS++LLLPLS+SLS S+DFNNTHNLLKSTAARSSARFHHRR
Sbjct: 1   MASPVFLFLLCFLISSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  HAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120
             H RSHLSLPLSPGGDYTLSFNLGSE+ KISLYMDTGSDLVWFPCSPFECILCEGKPKI
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYA 180
           QSPLPKIS  KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIE+SECSSFSCPPFYYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLSLPAPATA--PAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPS 240
           YGDGSLI +LYRDSLSLPAPA A  PAI+VRNFTFGCAH+ LGEP+GVAGFGRG LSMPS
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPS 240

Query: 241 QLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFY 300
           QLATFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYYG ETEF+YTS+LENPKHPYFY
Sbjct: 241 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFY 300

Query: 301 SVGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGR 360
           SVGLAGISVG+VRIPAPEFL+RVDEGGSGGVVVDSGTTFTMLPAGLY+SVVAQFENRTGR
Sbjct: 301 SVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGR 360

Query: 361 VASRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGR 420
           VASRASRIEENTGLSPCY Y+ SVEVPRVVLHFVGEKSSV LPRKNYFYEFLDGGDGVGR
Sbjct: 361 VASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDGVGR 420

Query: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLN 480
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLN
Sbjct: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLN 480

[Close]

BLAST of Spg016068 vs. NCBI nr
Match: XP_022923540.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])

HSP 1 Score: 888.3 bits (2294), Expect = 3.0e-254
Identity = 438/482 (90.87%), Postives = 458/482 (95.02%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRR 60
           M SPVFLFLLCFL  S VFSS++LLLPLS+SLS S+DFNNTHNLLKSTAARSSARFHHRR
Sbjct: 1   MASPVFLFLLCFLFSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  HAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120
             H RSHLSLPLSPGGDYTLSFNLGSE+ KISLYMDTGSDLVWFPCSPFECILCEGKPKI
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYA 180
           QSPLPKIS  KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIE+SECSSFSCPPFYYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLSLPAPATA--PAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPS 240
           YGDGSLI +LYRDSLSLPAPA A  PAI+VRNFTFGCAH+ LGEP+GVAGFGRG LSMPS
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPS 240

Query: 241 QLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFY 300
           QLATFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYYG ETEF+YTS+LENPKHPYFY
Sbjct: 241 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFY 300

Query: 301 SVGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGR 360
           SVGLAGISVG+VRIPAPEFL+RVDEGGSGGVVVDSGTTFTMLPAGLY+SVVAQFENRTGR
Sbjct: 301 SVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGR 360

Query: 361 VASRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGR 420
           VASRASRIEENTGLSPCY Y+ SVEVPRVVLHFVGEKSSV LPRKNYFYEFLDGGDGVGR
Sbjct: 361 VASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDGVGR 420

Query: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLN 480
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLN
Sbjct: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLN 480

[Close]

BLAST of Spg016068 vs. NCBI nr
Match: XP_038905814.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 883.2 bits (2281), Expect = 9.7e-253
Identity = 433/481 (90.02%), Postives = 457/481 (95.01%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFS-TDFNNTHNLLKSTAARSSARFHHR 60
           M S VF+ LLCFLL S VFSS++LLLPLSHSLS S +DFNNTHNLLKSTAARSSARFHHR
Sbjct: 1   MASSVFVLLLCFLLSSPVFSSQLLLLPLSHSLSSSISDFNNTHNLLKSTAARSSARFHHR 60

Query: 61  RHAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPK 120
           R     +HLSLPLSPGGDYTLSFNLGSE+HKISLYMDTGSDLVWFPCSPFECILCEGKPK
Sbjct: 61  RRTQHHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPK 120

Query: 121 IQSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYY 180
           +QSPLPKIS +KSVSCSA ACSAAHGGSLS+SHLCAIS CPL+SIEISECSSFSCPPFYY
Sbjct: 121 VQSPLPKISNNKSVSCSAPACSAAHGGSLSASHLCAISQCPLESIEISECSSFSCPPFYY 180

Query: 181 AYGDGSLIAQLYRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQ 240
           AYGDGSLIA+LYRDSLSLPAPA +PAI+VRNFTFGCAHT LGEPVGVAGFGRGTLSMPSQ
Sbjct: 181 AYGDGSLIARLYRDSLSLPAPAPSPAINVRNFTFGCAHTALGEPVGVAGFGRGTLSMPSQ 240

Query: 241 LATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYS 300
           LATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEF+YTSLLENPKHPYFYS
Sbjct: 241 LATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFIYTSLLENPKHPYFYS 300

Query: 301 VGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRV 360
           VGL GISVG + IPAPEFL++VDEGGSGGVVVDSGTTFTMLPAGLYDSVVA FENRTGRV
Sbjct: 301 VGLTGISVGNMMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAAFENRTGRV 360

Query: 361 ASRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRK 420
           A+RA RIEENTGLSPCYYY++SVEVPRVVLHFVGEKSSV+LP+KNYFYEFLDGGDGVG+K
Sbjct: 361 ANRARRIEENTGLSPCYYYENSVEVPRVVLHFVGEKSSVLLPKKNYFYEFLDGGDGVGKK 420

Query: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLNR 480
           RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDL KNRVGFARRQCSTLWDSLNR
Sbjct: 421 RKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLAKNRVGFARRQCSTLWDSLNR 480

[Close]

BLAST of Spg016068 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.8e-169
Identity = 299/477 (62.68%), Postives = 359/477 (75.26%), Query Frame = 0


Query: 24  LLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRRHAHRRSHLSLPLSPGGDYTLSFN 83
           LLL LSHSLS S   ++  +LLKS+++RSSARF    H  ++  LSLP+S G DY +S +
Sbjct: 29  LLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPISSGSDYLISLS 88

Query: 84  LGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKH-KSVSCSAAACSA 143
           +GS +  +SLY+DTGSDLVWFPC PF CILCE KP   SP   +S    +VSCS+ +CSA
Sbjct: 89  VGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSSATTVSCSSPSCSA 148

Query: 144 AHGGSLSSSHLCAISHCPLDSIEISEC--SSFSCPPFYYAYGDGSLIAQLYRDSLSLPAP 203
           AH  SL SS LCAIS+CPLD IE  +C  SS+ CPPFYYAYGDGSL+A+LY DSLSL   
Sbjct: 149 AH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSL--- 208

Query: 204 ATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQLATFSPQLGNRFSYCLVSHSF 263
              P++SV NFTFGCAHTTL EP+GVAGFGRG LS+P+QLA  SP LGN FSYCLVSHSF
Sbjct: 209 ---PSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSYCLVSHSF 268

Query: 264 AAERVRRPSPLILGRYYG--------------------GETEFVYTSLLENPKHPYFYSV 323
            ++RVRRPSPLILGR+                       + EFV+T +LENPKHPYFYSV
Sbjct: 269 DSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENPKHPYFYSV 328

Query: 324 GLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVA 383
            L GIS+G   IPAP  LRR+D+ G GGVVVDSGTTFTMLPA  Y+SVV +F++R GRV 
Sbjct: 329 SLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVH 388

Query: 384 SRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKR 443
            RA R+E ++G+SPCYY + +V+VP +VLHF G +SSV LPR+NYFYEF+DGGDG   KR
Sbjct: 389 ERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKR 448

Query: 444 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSL 478
           K+GCLMLMNGGDE+EL GG GA LGNYQQQGFEV+YDL   RVGFA+R+C++LWDSL
Sbjct: 449 KIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAKRKCASLWDSL 498

[Close]

BLAST of Spg016068 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.7e-34
Identity = 121/401 (30.17%), Postives = 171/401 (42.64%), Query Frame = 0


Query: 76  GDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKHKSVSC 135
           G+Y     +G+   ++ L +DTGSD+ W  C P  C  C  +          S +KS++C
Sbjct: 160 GEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP--CADCYQQSDPVFNPTSSSTYKSLTC 219

Query: 136 SAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAYGDGSL-IAQLYRDS 195
           SA  CS                      +E S C S  C  +  +YGDGS  + +L  D+
Sbjct: 220 SAPQCSL---------------------LETSACRSNKC-LYQVSYGDGSFTVGELATDT 279

Query: 196 LSLPAPATAPAISVRNFTFGCAHTTLG---EPVGVAGFGRGTLSMPSQLATFSPQLGNRF 255
           ++        +  + N   GC H   G      G+ G G G LS+ +Q+   S      F
Sbjct: 280 VTF-----GNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATS------F 339

Query: 256 SYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSVGLAGISVGTVR 315
           SYCLV            + + LG   GG+       LL N K   FY VGL+G SVG  +
Sbjct: 340 SYCLVDRDSGKSSSLDFNSVQLG---GGDAT---APLLRNKKIDTFYYVGLSGFSVGGEK 399

Query: 316 IPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVASRASRIEENTG 375
           +  P+ +  VD  GSGGV++D GT  T L    Y+S+   F   T  +   +S I   + 
Sbjct: 400 VVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSI---SL 459

Query: 376 LSPCYYYD--DSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKVGCLMLMN 435
              CY +    +V+VP V  HF G K S+ LP KNY     D G          C     
Sbjct: 460 FDTCYDFSSLSTVKVPTVAFHFTGGK-SLDLPAKNYLIPVDDSG--------TFCFAFAP 500

Query: 436 GGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQC 471
                 +       +GN QQQG  + YDL KN +G +  +C
Sbjct: 520 TSSSLSI-------IGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Spg016068 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 5.0e-34
Identity = 123/414 (29.71%), Postives = 185/414 (44.69%), Query Frame = 0


Query: 66  SHLSLPLSPG-GDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPL 125
           S +  P+  G G+Y ++  +G+ +   S  MDTGSDL+W  C P  C  C        P 
Sbjct: 83  SGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEP--CTQC-----FSQPT 142

Query: 126 PKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAYGDG 185
           P  +   S S S   C + +   L           P ++   +EC       + Y YGDG
Sbjct: 143 PIFNPQDSSSFSTLPCESQYCQDL-----------PSETCNNNECQ------YTYGYGDG 202

Query: 186 SLIAQLYRDSLSLPAPATAPAISVRNFTFGCAHTT----LGEPVGVAGFGRGTLSMPSQL 245
           S   Q Y  + +     T    SV N  FGC         G   G+ G G G LS+PSQL
Sbjct: 203 S-TTQGYMATETF----TFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQL 262

Query: 246 ATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETE-FVYTSLLENPKHPYFYS 305
                    +FSYC+ S+  ++     PS L LG    G  E    T+L+ +  +P +Y 
Sbjct: 263 GV------GQFSYCMTSYGSSS-----PSTLALGSAASGVPEGSPSTTLIHSSLNPTYYY 322

Query: 306 VGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRV 365
           + L GI+VG   +  P    ++ + G+GG+++DSGTT T LP   Y++V   F ++    
Sbjct: 323 ITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ---- 382

Query: 366 ASRASRIEENTGLSPCYYY---DDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGV 425
            +  +  E ++GLS C+       +V+VP + + F G    + L  +N      +G    
Sbjct: 383 INLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDG--GVLNLGEQNILISPAEG---- 435

Query: 426 GRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQC 471
                V CL +   G  ++L     +  GN QQQ  +VLYDL+   V F   QC
Sbjct: 443 -----VICLAM---GSSSQLG---ISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Spg016068 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.5e-33
Identity = 132/411 (32.12%), Postives = 183/411 (44.53%), Query Frame = 0


Query: 72  LSPG-GDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKH 131
           LS G G+Y     +G+    + + +DTGSD+VW  C+P  C  C  +       P     
Sbjct: 135 LSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAP--CRRCYSQSD-----PIFDPR 194

Query: 132 KSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFY-YAYGDGSL-IA 191
           KS + +   CS+ H   L S                + C++      Y  +YGDGS  + 
Sbjct: 195 KSKTYATIPCSSPHCRRLDS----------------AGCNTRRKTCLYQVSYGDGSFTVG 254

Query: 192 QLYRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVA---GFGRGTLSMPSQLATFSP 251
               ++L      T     V+    GC H   G  VG A   G G+G LS P Q      
Sbjct: 255 DFSTETL------TFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQT---GH 314

Query: 252 QLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSVGLAGI 311
           +   +FSYCLV  S ++    +PS ++ G          +T LL NPK   FY VGL GI
Sbjct: 315 RFNQKFSYCLVDRSASS----KPSSVVFGNAAVSRIA-RFTPLLSNPKLDTFYYVGLLGI 374

Query: 312 SVGTVRIP-APEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVASRAS 371
           SVG  R+P     L ++D+ G+GGV++DSGT+ T L    Y ++   F  R G  A    
Sbjct: 375 SVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--RVG--AKTLK 434

Query: 372 RIEENTGLSPCYYYD--DSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKV 431
           R  + +    C+     + V+VP VVLHF G  + V LP  NY                 
Sbjct: 435 RAPDFSLFDTCFDLSNMNEVKVPTVVLHFRG--ADVSLPATNYLIP-------------- 485

Query: 432 GCLMLMNGGDEAELAGGPG--ATLGNYQQQGFEVLYDLEKNRVGFARRQCS 472
              +  NG      AG  G  + +GN QQQGF V+YDL  +RVGFA   C+
Sbjct: 495 ---VDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Spg016068 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 4.2e-33
Identity = 120/404 (29.70%), Postives = 174/404 (43.07%), Query Frame = 0


Query: 76  GDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKHKSVSC 135
           G+Y ++ ++G+     S  MDTGSDL+W  C P  C  C          P  +   S S 
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQP--CTQC-----FNQSTPIFNPQGSSSF 152

Query: 136 SAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAYGDGSLIAQLYRDSL 195
           S   C         SS LC     P        CS+  C  + Y YGDGS      + S+
Sbjct: 153 STLPC---------SSQLCQALSSP-------TCSNNFC-QYTYGYGDGSET----QGSM 212

Query: 196 SLPAPATAPAISVRNFTFGCAHTT----LGEPVGVAGFGRGTLSMPSQLATFSPQLGNRF 255
                 T  ++S+ N TFGC         G   G+ G GRG LS+PSQL         +F
Sbjct: 213 GTET-LTFGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV------TKF 272

Query: 256 SYCLVSHSFAAERVRRPSPLILGRYYGGETE-FVYTSLLENPKHPYFYSVGLAGISVGTV 315
           SYC+     +      PS L+LG      T     T+L+++ + P FY + L G+SVG+ 
Sbjct: 273 SYCMTPIGSST-----PSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGST 332

Query: 316 RIPA-PEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVASRASRIEEN 375
           R+P  P         G+GG+++DSGTT T      Y SV  +F ++        S    +
Sbjct: 333 RLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGS----S 392

Query: 376 TGLSPCYYY---DDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKVGCLM 435
           +G   C+       ++++P  V+HF G    + LP +NYF    +G         + CL 
Sbjct: 393 SGFDLCFQTPSDPSNLQIPTFVMHFDG--GDLELPSENYFISPSNG---------LICLA 434

Query: 436 LMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQC 471
           + +      +        GN QQQ   V+YD   + V FA  QC
Sbjct: 453 MGSSSQGMSI-------FGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Spg016068 vs. ExPASy TrEMBL
Match: A0A6J1L3Z9 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.3e-255
Identity = 436/480 (90.83%), Postives = 459/480 (95.62%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRR 60
           M SPVFLFLLCFLLPS VFSS+ILLLPLS+SLS S+DFNNTHNLLKSTAARSSARFHHRR
Sbjct: 1   MASPVFLFLLCFLLPSPVFSSQILLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  HAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120
             H RSHLSLPLSPGGDYTLSFNLGSE+ KISLYMDTGSDLVWFPCSPFECILCEGKPKI
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYA 180
           QSPLPKIS  KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIE+SECSSFSCPPFYYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQL 240
           YGDGSLI +LYRDSLSLPAPA +PAI+VRNFTFGCAH+ LGEP+GVAGFGRG LSMP QL
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPIQL 240

Query: 241 ATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSV 300
           ATFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYYG ETEF+YTS+LENPKHPYFYSV
Sbjct: 241 ATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFYSV 300

Query: 301 GLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVA 360
           GLAGISVG+V IPAPEFL++VDEGGSGGVVVDSGTTFTMLPAGLY+SVVAQFENRTGRVA
Sbjct: 301 GLAGISVGSVMIPAPEFLKKVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGRVA 360

Query: 361 SRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKR 420
           SRAS+IEENTGLSPCYYY+ SVEVPRVVLHFVGEKSSV+LPRKNYFYEFLDGGDGVGRK 
Sbjct: 361 SRASQIEENTGLSPCYYYEKSVEVPRVVLHFVGEKSSVMLPRKNYFYEFLDGGDGVGRKI 420

Query: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLNRN 480
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLNR+
Sbjct: 421 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLNRS 480

[Close]

BLAST of Spg016068 vs. ExPASy TrEMBL
Match: A0A6J1EC44 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111431201 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 438/482 (90.87%), Postives = 458/482 (95.02%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRR 60
           M SPVFLFLLCFL  S VFSS++LLLPLS+SLS S+DFNNTHNLLKSTAARSSARFHHRR
Sbjct: 1   MASPVFLFLLCFLFSSPVFSSQLLLLPLSNSLSSSSDFNNTHNLLKSTAARSSARFHHRR 60

Query: 61  HAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120
             H RSHLSLPLSPGGDYTLSFNLGSE+ KISLYMDTGSDLVWFPCSPFECILCEGKPKI
Sbjct: 61  RTHHRSHLSLPLSPGGDYTLSFNLGSESQKISLYMDTGSDLVWFPCSPFECILCEGKPKI 120

Query: 121 QSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYA 180
           QSPLPKIS  KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIE+SECSSFSCPPFYYA
Sbjct: 121 QSPLPKISNQKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEVSECSSFSCPPFYYA 180

Query: 181 YGDGSLIAQLYRDSLSLPAPATA--PAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPS 240
           YGDGSLI +LYRDSLSLPAPA A  PAI+VRNFTFGCAH+ LGEP+GVAGFGRG LSMPS
Sbjct: 181 YGDGSLIGRLYRDSLSLPAPAPAPSPAINVRNFTFGCAHSALGEPIGVAGFGRGLLSMPS 240

Query: 241 QLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFY 300
           QLATFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYYG ETEF+YTS+LENPKHPYFY
Sbjct: 241 QLATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYGSETEFIYTSMLENPKHPYFY 300

Query: 301 SVGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGR 360
           SVGLAGISVG+VRIPAPEFL+RVDEGGSGGVVVDSGTTFTMLPAGLY+SVVAQFENRTGR
Sbjct: 301 SVGLAGISVGSVRIPAPEFLKRVDEGGSGGVVVDSGTTFTMLPAGLYNSVVAQFENRTGR 360

Query: 361 VASRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGR 420
           VASRASRIEENTGLSPCY Y+ SVEVPRVVLHFVGEKSSV LPRKNYFYEFLDGGDGVGR
Sbjct: 361 VASRASRIEENTGLSPCYSYEKSVEVPRVVLHFVGEKSSVELPRKNYFYEFLDGGDGVGR 420

Query: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLN 480
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV YDLE NRVGFARRQCSTLWDSLN
Sbjct: 421 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVAYDLENNRVGFARRQCSTLWDSLN 480

[Close]

BLAST of Spg016068 vs. ExPASy TrEMBL
Match: A0A0A0L5I7 (Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.1e-249
Identity = 429/480 (89.38%), Postives = 456/480 (95.00%), Query Frame = 0


Query: 3   SPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFS-TDFNNTHNLLKSTAARSSARFHHRRH 62
           SPVF+FLLCFLL S VFSS+I LLPLSHSLS S +DFNNTHNLLKSTA RSSARFH    
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNNTHNLLKSTATRSSARFHR--- 63

Query: 63  AHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 122
            HR +HLSLPLSPGGDYTLSFNLGSE+HKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ
Sbjct: 64  -HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 123

Query: 123 SPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAY 182
           SPLPKI+ +KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIEISECSSFSCPPFYYAY
Sbjct: 124 SPLPKIANNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAY 183

Query: 183 GDGSLIAQLYRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQLA 242
           GDGSL+A+LYRDSLSLP PA +P I+VRNFTFGCAHTTLGEPVGVAGFGRG LSMPSQLA
Sbjct: 184 GDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLA 243

Query: 243 TFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSVG 302
           TFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRYY GETEF+YTSLLENPKHPYFYSVG
Sbjct: 244 TFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYYTGETEFIYTSLLENPKHPYFYSVG 303

Query: 303 LAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVAS 362
           LAGISVG +RIPAPEFL +VDEGGSGGVVVDSGTTFTMLPAGLY+SVVA+FENRTG+VA+
Sbjct: 304 LAGISVGNIRIPAPEFLTKVDEGGSGGVVVDSGTTFTMLPAGLYESVVAEFENRTGKVAN 363

Query: 363 RASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDG-VGRKR 422
           RA RIEENTGLSPCYYY++SV VPRVVLHFVGEKS+VVLPRKNYFYEFLDGGDG VGRKR
Sbjct: 364 RARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSNVVLPRKNYFYEFLDGGDGVVGRKR 423

Query: 423 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLNRN 481
           KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV+YDLEKNRVGFARRQCSTLWD+LNR+
Sbjct: 424 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLNRS 479

[Close]

BLAST of Spg016068 vs. ExPASy TrEMBL
Match: A0A1S3BK28 (aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 3.5e-248
Identity = 429/482 (89.00%), Postives = 456/482 (94.61%), Query Frame = 0


Query: 3   SPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFS-TDFNNTHNLLKSTAARSSARFHHRRH 62
           SPVF+FLLCFLL S VFSS+I LLPLSHSLS S +DFN+THNLLKSTA RSSARFH    
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR--- 63

Query: 63  AHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 122
            HR +HLSLPLSPGGDYTLSFNLGSE+HKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ
Sbjct: 64  -HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 123

Query: 123 SPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAY 182
           SPLPKIS +KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIEISECSSFSCPPFYYAY
Sbjct: 124 SPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAY 183

Query: 183 GDGSLIAQLYRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQLA 242
           GDGSL+A+LYRDSLSLP PA +P I+VRNFTFGCAHTTLGEPVGVAGFGRG LSMPSQLA
Sbjct: 184 GDGSLVARLYRDSLSLPTPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQLA 243

Query: 243 TFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSVG 302
           TFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRY+ GETEF+YTSLLENPKHPYFYSVG
Sbjct: 244 TFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYSVG 303

Query: 303 LAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVAS 362
           LAGISVG VRIPAPEFLR+VDE GSGGVVVDSGTTFTMLP+GLY+SVVA+FENRTG+VA+
Sbjct: 304 LAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKVAN 363

Query: 363 RASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDG---VGR 422
           RA RIEENTGLSPCYYY++SV VPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDG   VGR
Sbjct: 364 RARRIEENTGLSPCYYYENSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEVGR 423

Query: 423 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSLN 481
           KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV+YDLEKNRVGFARRQCSTLWD+LN
Sbjct: 424 KRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDNLN 481

[Close]

BLAST of Spg016068 vs. ExPASy TrEMBL
Match: A0A5D3CP11 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00280 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.1e-247
Identity = 430/484 (88.84%), Postives = 456/484 (94.21%), Query Frame = 0


Query: 3   SPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFS-TDFNNTHNLLKSTAARSSARFHHRRH 62
           SPVF+FLLCFLL S VFSS+I LLPLSHSLS S +DFN+THNLLKSTA RSSARFH    
Sbjct: 4   SPVFIFLLCFLLSSPVFSSQIFLLPLSHSLSSSISDFNSTHNLLKSTATRSSARFHR--- 63

Query: 63  AHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 122
            HR +HLSLPLSPGGDYTLSFNLGSE+HKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ
Sbjct: 64  -HRHNHLSLPLSPGGDYTLSFNLGSESHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQ 123

Query: 123 SPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAY 182
           SPLPKIS +KSVSCSAAACSAAHGGSLS+SHLCAIS CPL+SIEISECSSFSCPPFYYAY
Sbjct: 124 SPLPKISNNKSVSCSAAACSAAHGGSLSASHLCAISRCPLESIEISECSSFSCPPFYYAY 183

Query: 183 GDGSLIAQLYRDSLSLPAPATAPA--ISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQ 242
           GDGSL+A+LYRDSLSLP PA AP+  I+VRNFTFGCAHTTLGEPVGVAGFGRG LSMPSQ
Sbjct: 184 GDGSLVARLYRDSLSLPTPAPAPSPPINVRNFTFGCAHTTLGEPVGVAGFGRGVLSMPSQ 243

Query: 243 LATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYS 302
           LATFSPQLGNRFSYCLVSHSFAA+RVRRPSPLILGRY+ GETEF+YTSLLENPKHPYFYS
Sbjct: 244 LATFSPQLGNRFSYCLVSHSFAADRVRRPSPLILGRYHTGETEFIYTSLLENPKHPYFYS 303

Query: 303 VGLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRV 362
           VGLAGISVG VRIPAPEFLR+VDE GSGGVVVDSGTTFTMLP+GLY+SVVA+FENRTG+V
Sbjct: 304 VGLAGISVGNVRIPAPEFLRKVDESGSGGVVVDSGTTFTMLPSGLYESVVAEFENRTGKV 363

Query: 363 ASRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDG---V 422
           A+RA RIEENTGLSPCYYY +SV VPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDG   V
Sbjct: 364 ANRARRIEENTGLSPCYYYQNSVGVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVVEV 423

Query: 423 GRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDS 481
           GRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEV+YDLEKNRVGFARRQCSTLWD+
Sbjct: 424 GRKRKVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVVYDLEKNRVGFARRQCSTLWDN 483

[Close]

BLAST of Spg016068 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 597.0 bits (1538), Expect = 1.3e-170
Identity = 299/477 (62.68%), Postives = 359/477 (75.26%), Query Frame = 0


Query: 24  LLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRRHAHRRSHLSLPLSPGGDYTLSFN 83
           LLL LSHSLS S   ++  +LLKS+++RSSARF    H  ++  LSLP+S G DY +S +
Sbjct: 29  LLLHLSHSLSTSKHSSSPLHLLKSSSSRSSARFRRHHHKQQQQQLSLPISSGSDYLISLS 88

Query: 84  LGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKH-KSVSCSAAACSA 143
           +GS +  +SLY+DTGSDLVWFPC PF CILCE KP   SP   +S    +VSCS+ +CSA
Sbjct: 89  VGSSSSAVSLYLDTGSDLVWFPCRPFTCILCESKPLPPSPPSSLSSSATTVSCSSPSCSA 148

Query: 144 AHGGSLSSSHLCAISHCPLDSIEISEC--SSFSCPPFYYAYGDGSLIAQLYRDSLSLPAP 203
           AH  SL SS LCAIS+CPLD IE  +C  SS+ CPPFYYAYGDGSL+A+LY DSLSL   
Sbjct: 149 AH-SSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSLVAKLYSDSLSL--- 208

Query: 204 ATAPAISVRNFTFGCAHTTLGEPVGVAGFGRGTLSMPSQLATFSPQLGNRFSYCLVSHSF 263
              P++SV NFTFGCAHTTL EP+GVAGFGRG LS+P+QLA  SP LGN FSYCLVSHSF
Sbjct: 209 ---PSVSVSNFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVHSPHLGNSFSYCLVSHSF 268

Query: 264 AAERVRRPSPLILGRYYG--------------------GETEFVYTSLLENPKHPYFYSV 323
            ++RVRRPSPLILGR+                       + EFV+T +LENPKHPYFYSV
Sbjct: 269 DSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEFVFTEMLENPKHPYFYSV 328

Query: 324 GLAGISVGTVRIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVA 383
            L GIS+G   IPAP  LRR+D+ G GGVVVDSGTTFTMLPA  Y+SVV +F++R GRV 
Sbjct: 329 SLQGISIGKRNIPAPAMLRRIDKNGGGGVVVDSGTTFTMLPAKFYNSVVEEFDSRVGRVH 388

Query: 384 SRASRIEENTGLSPCYYYDDSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKR 443
            RA R+E ++G+SPCYY + +V+VP +VLHF G +SSV LPR+NYFYEF+DGGDG   KR
Sbjct: 389 ERADRVEPSSGMSPCYYLNQTVKVPALVLHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKR 448

Query: 444 KVGCLMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQCSTLWDSL 478
           K+GCLMLMNGGDE+EL GG GA LGNYQQQGFEV+YDL   RVGFA+R+C++LWDSL
Sbjct: 449 KIGCLMLMNGGDESELRGGTGAILGNYQQQGFEVVYDLLNRRVGFAKRKCASLWDSL 498

[Close]

BLAST of Spg016068 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 202.6 bits (514), Expect = 7.1e-52
Identity = 164/510 (32.16%), Postives = 229/510 (44.90%), Query Frame = 0


Query: 2   TSPVFLFLLCFLL--------------PSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKS 61
           T  +FLFLL  LL              PSS  SS ++L     S+S  T  + T   +K 
Sbjct: 5   THVLFLFLLITLLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPKSQTQERIKK 64

Query: 62  TAARSSARFHHRRHAHRRSHLSLPLSPGGDYTLSFNLGSENHKISLYMDTGSDLVWFPCS 121
             +         R                 Y ++ N+G+    + +Y+DTGSDL W PC 
Sbjct: 65  PLSSVDVVMEPLREVR------------DGYLITLNIGTPPQAVQVYLDTGSDLTWVPCG 124

Query: 122 --PFECILCEG-------KPKIQSPLPKISKHKSVSCSAAACSAAHGGSLSSSHLCAISH 181
              F+CI C          P + SPL   +  +  SC+++ C   H  S +    CA++ 
Sbjct: 125 NLSFDCIECYDLKNNDLKSPSVFSPLHSSTSFRD-SCASSFCVEIH-SSDNPFDPCAVAG 184

Query: 182 CPLDSIEISECSSFSCPPFYYAYGDGSLIAQ-LYRDSLSLPAPATAPAISVRNFTFGCAH 241
           C +  +  S C    CP F Y YG+G LI+  L RD L       A    V  F+FGC  
Sbjct: 185 CSVSMLLKSTCVR-PCPSFAYTYGEGGLISGILTRDILK------ARTRDVPRFSFGCVT 244

Query: 242 TTLGEPVGVAGFGRGTLSMPSQLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGR-- 301
           +T  EP+G+AGFGRG LS+PSQL      L   FS+C +   F        SPLILG   
Sbjct: 245 STYREPIGIAGFGRGLLSLPSQLGF----LEKGFSHCFLPFKF-VNNPNISSPLILGASA 304

Query: 302 -YYGGETEFVYTSLLENPKHPYFYSVGLAGISVGTVRIP--APEFLRRVDEGGSGGVVVD 361
                     +T +L  P +P  Y +GL  I++GT   P   P  LR+ D  G+GG++VD
Sbjct: 305 LSINLTDSLQFTPMLNTPMYPNSYYIGLESITIGTNITPTQVPLTLRQFDSQGNGGMLVD 364

Query: 362 SGTTFTMLPAGLYDSVVAQFENRTGRVASRASRIEENTGLSPCYYY------------DD 421
           SGTT+T LP   Y  ++   ++       RA+  E  TG   CY              D 
Sbjct: 365 SGTTYTHLPEPFYSQLLTTLQSTI--TYPRATETESRTGFDLCYKVPCPNNNLTSLENDV 424

Query: 422 SVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKVGCLMLMNGGDEAELAGGP 471
            +  P +  HF+   ++++LP+ N FY      DG      V CL+  N  D      GP
Sbjct: 425 MMIFPSITFHFL-NNATLLLPQGNSFYAMSAPSDG----SVVQCLLFQNMEDGDY---GP 478

[Close]

BLAST of Spg016068 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 174.5 bits (441), Expect = 2.1e-43
Identity = 152/505 (30.10%), Postives = 224/505 (44.36%), Query Frame = 0


Query: 1   MTSPVFLFLLCFLLPSSVFSSEILLLPLSHSLSFSTDFNNTHNLLKSTAARSSARFHHRR 60
           M S +F F L FL  S V + ++ L P SHS     D    +  L+  A  S AR H  +
Sbjct: 1   MASSIFFFFLIFL--SVVSAVKLPLSPFSHSDQSPKD---PYLSLRRLAESSIARAHKLK 60

Query: 61  H---------------AHRRSHLSLPLSPG--GDYTLSFNLGSENHKISLYMDTGSDLVW 120
           H                   + +  PLS    G Y++S + G+ +  I    DTGS LVW
Sbjct: 61  HGTSIKPDEDALSSTTTASATVVKSPLSAKSYGGYSVSLSFGTPSQTIPFVFDTGSSLVW 120

Query: 121 FPC-SPFECILCEGKPKIQSPLPKI-----SKHKSVSCSAAACSAAHGGSLSSSHLCAIS 180
            PC S + C  C+      + +P+      S  K + C +  C   +G ++         
Sbjct: 121 LPCTSRYLCSGCDFSGLDPTLIPRFIPKNSSSSKIIGCQSPKCQFLYGPNV--------- 180

Query: 181 HCPLDSIEISECSSFSCPPFYYAYGDGSLIAQLYRDSLSLPAPATAPAISVRNFTFGCAH 240
            C         C +  CPP+   YG GS    L  + L        P ++V +F  GC+ 
Sbjct: 181 QCRGCDPNTRNC-TVGCPPYILQYGLGSTAGVLITEKLDF------PDLTVPDFVVGCSI 240

Query: 241 TTLGEPVGVAGFGRGTLSMPSQLATFSPQLGNRFSYCLVSHSFAAERVRRPSPLILGRYY 300
            +  +P G+AGFGRG +S+PSQ+         RFS+CLVS  F    V     L  G  +
Sbjct: 241 ISTRQPAGIAGFGRGPVSLPSQMNL------KRFSHCLVSRRFDDTNVTTDLDLDTGSGH 300

Query: 301 --GGETE-FVYTSLLENPK-----HPYFYSVGLAGISVGTVRIPAPEFLRRVDEGGSGGV 360
             G +T    YT   +NP         +Y + L  I VG   +  P         G GG 
Sbjct: 301 NSGSKTPGLTYTPFRKNPNVSNKAFLEYYYLNLRRIYVGRKHVKIPYKYLAPGTNGDGGS 360

Query: 361 VVDSGTTFTMLPAGLYDSVVAQFENRTGRVASRASRIEENTGLSPCYYYD--DSVEVPRV 420
           +VDSG+TFT +   +++ V  +F ++     +R   +E+ TGL PC+       V VP +
Sbjct: 361 IVDSGSTFTFMERPVFELVAEEFASQMSNY-TREKDLEKETGLGPCFNISGKGDVTVPEL 420

Query: 421 VLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKVGCLMLMNGGDEAELAG-GPGATLGN 472
           +  F G  + + LP  NYF  F+   D V       CL +++        G GP   LG+
Sbjct: 421 IFEFKG-GAKLELPLSNYF-TFVGNTDTV-------CLTVVSDKTVNPSGGTGPAIILGS 468

[Close]

BLAST of Spg016068 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 150.2 bits (378), Expect = 4.2e-36
Identity = 129/406 (31.77%), Postives = 174/406 (42.86%), Query Frame = 0


Query: 72  LSPG-GDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKH 131
           LS G G+Y +   +G+    + + +DTGSD+VW  CSP  C  C  +        K    
Sbjct: 128 LSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSP--CKACYNQTDAIFDPKKSKTF 187

Query: 132 KSVSCSAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAYGDGSLIAQL 191
            +V C +  C                     DS E     S +C  +  +YGDGS     
Sbjct: 188 ATVPCGSRLCRRLD-----------------DSSECVTRRSKTC-LYQVSYGDGS----- 247

Query: 192 YRDSLSLPAPATAPAISVRNFTFGCAHTTLGEPVGVA---GFGRGTLSMPSQLATFSPQL 251
           + +        T     V +   GC H   G  VG A   G GRG LS PSQ      + 
Sbjct: 248 FTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKN---RY 307

Query: 252 GNRFSYCLVSHSFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSVGLAGISV 311
             +FSYCLV  + +    + PS ++ G     +T  V+T LL NPK   FY + L GISV
Sbjct: 308 NGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTS-VFTPLLTNPKLDTFYYLQLLGISV 367

Query: 312 GTVRIP-APEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVASRASRI 371
           G  R+P   E   ++D  G+GGV++DSGT+ T L    Y ++   F  R G  A++  R 
Sbjct: 368 GGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAF--RLG--ATKLKRA 427

Query: 372 EENTGLSPCYYYD--DSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKVGC 431
              +    C+      +V+VP VV HF G    V LP  NY                 G 
Sbjct: 428 PSYSLFDTCFDLSGMTTVKVPTVVFHFGG--GEVSLPASNYLIPV----------NTEGR 483

Query: 432 LMLMNGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQC 471
                 G    L     + +GN QQQGF V YDL  +RVGF  R C
Sbjct: 488 FCFAFAGTMGSL-----SIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of Spg016068 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 149.1 bits (375), Expect = 9.4e-36
Identity = 125/402 (31.09%), Postives = 177/402 (44.03%), Query Frame = 0


Query: 76  GDYTLSFNLGSENHKISLYMDTGSDLVWFPCSPFECILCEGKPKIQSPLPKISKHKSVSC 135
           G+Y     +G    ++ + +DTGSD+ W  C+P  C  C  + +        S ++ +SC
Sbjct: 146 GEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP--CADCYHQTEPIFEPSSSSSYEPLSC 205

Query: 136 SAAACSAAHGGSLSSSHLCAISHCPLDSIEISECSSFSCPPFYYAYGDGS-LIAQLYRDS 195
               C+A                     +E+SEC + +C  +  +YGDGS  +     ++
Sbjct: 206 DTPQCNA---------------------LEVSECRNATC-LYEVSYGDGSYTVGDFATET 265

Query: 196 LSLPAPATAPAISVRNFTFGCAHTTLGEPVGVA---GFGRGTLSMPSQLATFSPQLGNRF 255
           L      T  +  V+N   GC H+  G  VG A   G G G L++PSQL T S      F
Sbjct: 266 L------TIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTS------F 325

Query: 256 SYCLVSH-SFAAERVRRPSPLILGRYYGGETEFVYTSLLENPKHPYFYSVGLAGISVGTV 315
           SYCLV   S +A  V   + L          + V   LL N +   FY +GL GISVG  
Sbjct: 326 SYCLVDRDSDSASTVDFGTSL--------SPDAVVAPLLRNHQLDTFYYLGLTGISVGGE 385

Query: 316 RIPAPEFLRRVDEGGSGGVVVDSGTTFTMLPAGLYDSVVAQFENRTGRVASRASRIEENT 375
            +  P+    +DE GSGG+++DSGT  T L   +Y+S+   F   T  +   A     +T
Sbjct: 386 LLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDT 445

Query: 376 GLSPCYYYD--DSVEVPRVVLHFVGEKSSVVLPRKNYFYEFLDGGDGVGRKRKVGCLMLM 435
               CY      +VEVP V  HF G K  + LP KNY        D VG      CL   
Sbjct: 446 ----CYNLSAKTTVEVPTVAFHFPGGK-MLALPAKNYMIPV----DSVG----TFCLAFA 483

Query: 436 NGGDEAELAGGPGATLGNYQQQGFEVLYDLEKNRVGFARRQC 471
                        A +GN QQQG  V +DL  + +GF+  +C
Sbjct: 506 PTASSL-------AIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

The following BLAST results are available for this feature:

BLAST of Spg016068 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023553227.1	2.1e-255	91.29	probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo]	[more]
XP_023007805.1	2.7e-255	90.83	probable aspartyl protease At4g16563 [Cucurbita maxima]	[more]
KAG6577689.1	1.8e-254	90.87	putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022923540.1	3.0e-254	90.87	probable aspartyl protease At4g16563 [Cucurbita moschata]	[more]
XP_038905814.1	9.7e-253	90.02	probable aspartyl protease At4g16563 [Benincasa hispida]	[more]

BLAST of Spg016068 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940R4	1.8e-169	62.68	Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656...	[more]
Q9LS40	1.7e-34	30.17	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C2	5.0e-34	29.71	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	1.5e-33	32.12	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	4.2e-33	29.70	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of Spg016068 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L3Z9	1.3e-255	90.83	probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111500303...	[more]
A0A6J1EC44	1.5e-254	90.87	probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114312...	[more]
A0A0A0L5I7	4.1e-249	89.38	Pepsin A OS=Cucumis sativus OX=3659 GN=Csa_3G020060 PE=3 SV=1	[more]
A0A1S3BK28	3.5e-248	89.00	aspartic proteinase nepenthesin-1 OS=Cucumis melo OX=3656 GN=LOC103490888 PE=3 S...	[more]
A0A5D3CP11	5.1e-247	88.84	Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of Spg016068 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16563.1	1.3e-170	62.68	Eukaryotic aspartyl protease family protein	[more]
AT5G45120.1	7.1e-52	32.16	Eukaryotic aspartyl protease family protein	[more]
AT3G52500.1	2.1e-43	30.10	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	4.2e-36	31.77	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	9.4e-36	31.09	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 297..466 e-value: 5.4E-27 score: 94.5
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 78..261 e-value: 6.8E-30 score: 104.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 57..263 e-value: 7.6E-35 score: 122.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 277..476 e-value: 2.4E-47 score: 162.9
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 73..475
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 2..474
None	No IPR available	PANTHER	PTHR47967:SF26	BNAA01G17170D PROTEIN	coord: 2..474
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 329..340
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 78..466 score: 29.930611
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 78..470 e-value: 5.70284E-69 score: 219.442

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg016068.1	Spg016068.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity