Spg016034 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg016034 vs. NCBI nr
Match: XP_038876802.1 (IRK-interacting protein-like [Benincasa hispida])

HSP 1 Score: 798.9 bits (2062), Expect = 2.3e-227
Identity = 418/470 (88.94%), Postives = 430/470 (91.49%), Query Frame = 0

Query: 1   MKNITPSSSPSPLHHCQSSPFFSITQNSERT-EEEDEFNRATPSSRTDAGAKHNPTPLHH 60
           MKNITPS          SSPF S    +ERT EEEDEFNRATPSS T AGAKH+PTPLHH
Sbjct: 1   MKNITPS----------SSPFCSFIHKTERTEEEEDEFNRATPSSGTYAGAKHHPTPLHH 60

Query: 61  HSHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120
           H    GGDGILPKRSTKKRPESAASV  GGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF
Sbjct: 61  H----GGDGILPKRSTKKRPESAASV-SGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120

Query: 121 SMASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAIL 180
           S+ASPNGILKSIVSS TRKSPKSMNE TDW AREEHWKIALGEISHKLIQATRKRDEAIL
Sbjct: 121 SIASPNGILKSIVSSLTRKSPKSMNEGTDWAAREEHWKIALGEISHKLIQATRKRDEAIL 180

Query: 181 EASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFL 240
           EASRLKYSMAELEKKLNKLEIYCH+LKS LDECASNPRKPKQEV TNSD ISD+IIENFL
Sbjct: 181 EASRLKYSMAELEKKLNKLEIYCHSLKSELDECASNPRKPKQEVLTNSDSISDKIIENFL 240

Query: 241 SSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHLEAI 300
           SSVSESRSSVRQLSRSLAMQLR MGGKI+ERIQ LLQSHEIK   SK+PRTSL+FHLEAI
Sbjct: 241 SSVSESRSSVRQLSRSLAMQLRQMGGKIYERIQFLLQSHEIKIPLSKNPRTSLIFHLEAI 300

Query: 301 LNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFS 360
           LNRAFFEDFESIGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVL+KGTRHFSE+FS
Sbjct: 301 LNRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLNKGTRHFSEDFS 360

Query: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARF 420
           RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG RF
Sbjct: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRF 420

Query: 421 DGVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           DGVYMEDIAG+K REL+PA VRIMVSPGFYVFDNLIKCKVLCRYH NLNN
Sbjct: 421 DGVYMEDIAGDKARELSPAAVRIMVSPGFYVFDNLIKCKVLCRYHINLNN 455

BLAST of Spg016034 vs. NCBI nr
Match: XP_023552161.1 (IRK-interacting protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798.1 bits (2060), Expect = 4.0e-227
Identity = 409/470 (87.02%), Postives = 428/470 (91.06%), Query Frame = 0

Query: 1   MKNITPSSSPS-PLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHH 60
           MKNITPSSSPS PLHH QSSPFFSI  NSERTEEEDEFNRATPSS TDAGAKH+PTPLH 
Sbjct: 1   MKNITPSSSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPSSCTDAGAKHHPTPLHL 60

Query: 61  HSHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120
           HSH  GGD ILPKRSTKKR ES AS+GGGGSVSCSKCRPHAREKFSVVP+D NGVAKQFF
Sbjct: 61  HSH--GGDEILPKRSTKKRLESVASIGGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFF 120

Query: 121 SMASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAIL 180
           SMASPNGILKSIVSS T KSPKSM E  DW  REE WKIA GE+SHKLI+ATRKRDEAIL
Sbjct: 121 SMASPNGILKSIVSSLTGKSPKSMKEGGDWAVREEQWKIAFGEVSHKLIEATRKRDEAIL 180

Query: 181 EASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFL 240
           EASRLKYSMAELEKKLNKLEIYCH+LKS L +C SNPR P  EVPTNSD ISD+IIENFL
Sbjct: 181 EASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFL 240

Query: 241 SSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHLEAI 300
           SSVSESRSSVRQLSRSLAMQL H+GGKI+ERIQ LL+SHEIK   SK+ +TSL+FHLEAI
Sbjct: 241 SSVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAI 300

Query: 301 LNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFS 360
           LNRAFFEDFESIGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVL KGTRHFSE+FS
Sbjct: 301 LNRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFS 360

Query: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARF 420
           RFCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RF
Sbjct: 361 RFCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRF 420

Query: 421 DGVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           DGVYMEDIAGEK RELAPATVRIMV+PGFY FD LIKCKV+CRYH+NLNN
Sbjct: 421 DGVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYHTNLNN 468

BLAST of Spg016034 vs. NCBI nr
Match: XP_022923173.1 (IRK-interacting protein-like [Cucurbita moschata])

HSP 1 Score: 792.7 bits (2046), Expect = 1.7e-225
Identity = 406/469 (86.57%), Postives = 424/469 (90.41%), Query Frame = 0

Query: 1   MKNITPSSSPSPLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHHH 60
           MKNITPS SP PLHH QSSPFFSI  NSERTEEEDEFNRATP S TDAGAKH+PTPLH H
Sbjct: 1   MKNITPSPSP-PLHHYQSSPFFSIVPNSERTEEEDEFNRATPGSCTDAGAKHHPTPLHLH 60

Query: 61  SHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFS 120
           SH  GGD ILPKRSTKKR E  ASVGGGGSVSCSKCRPHAREKFSVVP+D NGVAKQFFS
Sbjct: 61  SH--GGDEILPKRSTKKRLEPVASVGGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFS 120

Query: 121 MASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAILE 180
           MASPNGILKSIVSS T KSPKSM E  DW  REE WKIA GE+SHKLI+ATRKRDEAILE
Sbjct: 121 MASPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFGEVSHKLIEATRKRDEAILE 180

Query: 181 ASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFLS 240
           ASRLKYSMAELEKKLNKLEIYCH+LKS L +C SNPR P  EVPTNSD ISD+IIENFLS
Sbjct: 181 ASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLS 240

Query: 241 SVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHLEAIL 300
           SVSESRSSVRQLSRSLAMQL H+GGKI+ERIQ LL+SHEIK   SK+ +TSL+FHLEAIL
Sbjct: 241 SVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAIL 300

Query: 301 NRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFSR 360
           NRAFFEDFESIGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVL KGTRHFSE+FSR
Sbjct: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSR 360

Query: 361 FCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFD 420
           FCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RFD
Sbjct: 361 FCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFD 420

Query: 421 GVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           GVYMEDIAGEK RELAPATVRIMV+PGFY FD LIKCKV+CRYH+NLNN
Sbjct: 421 GVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYHTNLNN 466

BLAST of Spg016034 vs. NCBI nr
Match: XP_022965043.1 (IRK-interacting protein-like [Cucurbita maxima])

HSP 1 Score: 792.3 bits (2045), Expect = 2.2e-225
Identity = 406/470 (86.38%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   MKNITPSSSPS-PLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHH 60
           MKNITPSSSPS PLHH QSSPFFSI  NSERTEEEDEFNRATPSS TD GAKH+PTPLH 
Sbjct: 1   MKNITPSSSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPSSCTDDGAKHHPTPLHL 60

Query: 61  HSHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120
           HSH  GGD ILPKRSTKKR E  ASVG GGSVSCSKCRPHAREKFSVVP+D NGVAKQFF
Sbjct: 61  HSH--GGDEILPKRSTKKRLEPVASVGDGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFF 120

Query: 121 SMASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAIL 180
           SMASPNGILKSIVSSFT KSPKSM E  DW  REEHWKIA GE+SHKLI+ATRKRDEAIL
Sbjct: 121 SMASPNGILKSIVSSFTGKSPKSMKEGADWAVREEHWKIAFGEVSHKLIEATRKRDEAIL 180

Query: 181 EASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFL 240
           EASRLKYSMAELEKKLNKLEIYCH+LKS L +C SNPR P  EVPTNSD ISD+IIENFL
Sbjct: 181 EASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFL 240

Query: 241 SSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHLEAI 300
           SSVSESRSS+RQLSRSLAMQL H+G KI+ERIQ LL+SH+IK   SK+ +TSL+FHLEAI
Sbjct: 241 SSVSESRSSMRQLSRSLAMQLHHIGDKIYERIQFLLRSHDIKISLSKNSKTSLIFHLEAI 300

Query: 301 LNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFS 360
           LNRAFFEDFESIGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVL KGTRHFSE+FS
Sbjct: 301 LNRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFS 360

Query: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARF 420
           RFCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLATAVHPSLPIFRVDSG RF
Sbjct: 361 RFCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLATAVHPSLPIFRVDSGVRF 420

Query: 421 DGVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           DGVYMEDIAG+K RELAPATVRIMV+PGFY FD LIKCKV CRYH+NLNN
Sbjct: 421 DGVYMEDIAGDKARELAPATVRIMVAPGFYAFDGLIKCKVTCRYHTNLNN 468

BLAST of Spg016034 vs. NCBI nr
Match: KAG6577593.1 (IRK-interacting protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 792.3 bits (2045), Expect = 2.2e-225
Identity = 405/469 (86.35%), Postives = 423/469 (90.19%), Query Frame = 0

Query: 1   MKNITPSSSPSPLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHHH 60
           MKNITPSS   PLHH QSSPFFSI  NSERTEEEDEFNRATPSS TDAGAKH+PTPLH H
Sbjct: 1   MKNITPSSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPSSCTDAGAKHHPTPLHLH 60

Query: 61  SHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFS 120
           SH  GGD ILPKRSTKKR E  ASVGGGGSVSCSKCRPHAREKFSVVP+D NGVAKQFFS
Sbjct: 61  SH--GGDEILPKRSTKKRLEPVASVGGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFS 120

Query: 121 MASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAILE 180
           MASPNGILKSIVSS T KSPKSM E  DW  REE WKIA  E+SHKLI+ATRKRDEAILE
Sbjct: 121 MASPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFDEVSHKLIEATRKRDEAILE 180

Query: 181 ASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFLS 240
           ASRLKYSMAELEKKLNKLEIYCH+LKS L +C SNPR P  EVPTNSD ISD+IIENFLS
Sbjct: 181 ASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLS 240

Query: 241 SVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHLEAIL 300
           SVSESRSSVRQLSRSLAMQL H+GGKI+ERIQ LL+SHEIK   SK+ +TSL+FHLEAIL
Sbjct: 241 SVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAIL 300

Query: 301 NRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFSR 360
           NRAFFEDFESIGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVL KGTRHFSE+FSR
Sbjct: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSR 360

Query: 361 FCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFD 420
           FCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RFD
Sbjct: 361 FCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFD 420

Query: 421 GVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           GVYMEDIAGEK RELAPATVRIMV+PGFY FD LIKCKV+CRYH+NLNN
Sbjct: 421 GVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYHTNLNN 467

BLAST of Spg016034 vs. ExPASy Swiss-Prot
Match: Q9LXU9 (IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.5e-64
Identity = 128/311 (41.16%), Postives = 189/311 (60.77%), Query Frame = 0

Query: 162 EISHKLIQATRKRDEAILEASRLKYSMAELEKKLNKLEIYCHTLKSGLDECA-----SNP 221
           ++  +L++A R RD A+ + S +K S+ EL +KL  LE YC  LK  L E        N 
Sbjct: 250 KLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNLKKALREATEVVSQENS 309

Query: 222 RKPKQEVPTNSDPISDRI-IENFLSSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILL 281
                    +  P+S+ + +E FL  VSE+R S++Q  ++L  ++      +   I  LL
Sbjct: 310 GGRSSGKKNSEMPVSEEVMVEGFLQIVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLL 369

Query: 282 QSHEIKSKSPRTSLM-FHLEAILNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNR 341
           Q H +   S  + ++ +HLEAI++++ ++DFE+  FQKN    +L+P    ++N +SF  
Sbjct: 370 QPHNLSFTSKYSKIIQYHLEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFAS 429

Query: 342 LHRLSWEEVLSKGTRHFSEEFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLV 401
           L  LSW EVL KGT+++S+EFSRFCD KMS I+  L W R W E +LQAFF AAK VWL+
Sbjct: 430 LRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVWLL 489

Query: 402 HLLATAVHPSLPIFRVDSGARFDGVYMEDIAGEKTREL---APATVRIMVSPGFYVFDNL 461
           HLLA + +P+L I RV+    F+  +MED+  ++ R      PA V++MV PGFYV D +
Sbjct: 490 HLLAFSFNPALGILRVEENREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFYVLDRV 549

Query: 462 IKCKVLCRYHS 463
           ++CKVLCRY S
Sbjct: 550 LRCKVLCRYKS 560

BLAST of Spg016034 vs. ExPASy Swiss-Prot
Match: F4KGE8 (Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.0e-07
Identity = 53/184 (28.80%), Postives = 74/184 (40.22%), Query Frame = 0

Query: 280 IKSKSPRTSLMFHLEAILNRAFFEDFESIGF-QKNSPNPILNPSDRTESNIASFNRLHRL 339
           ++S  P     F LE+ + R  F+ F+   F    S + ++NP        A F  +  +
Sbjct: 359 VQSSVPNQHAKFALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAM 418

Query: 340 SWEEVLS-KGTRHFSEEFSRFCDRKMSDIV------------AMLEWNRAWPEPLLQ--- 399
              E+L    T H    F +FC +K   I+               E   A   P  Q   
Sbjct: 419 DPMELLGILPTCH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYG 478

Query: 400 AFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIAG-EKTRELAPATVRIMV 446
            F   AK+VWL+HLLA ++ PS   F  + GA F   YME +      R  A   V   V
Sbjct: 479 EFLGLAKAVWLLHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPV 538

BLAST of Spg016034 vs. ExPASy TrEMBL
Match: A0A6J1E8U8 (IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430947 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 8.1e-226
Identity = 406/469 (86.57%), Postives = 424/469 (90.41%), Query Frame = 0

Query: 1   MKNITPSSSPSPLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHHH 60
           MKNITPS SP PLHH QSSPFFSI  NSERTEEEDEFNRATP S TDAGAKH+PTPLH H
Sbjct: 1   MKNITPSPSP-PLHHYQSSPFFSIVPNSERTEEEDEFNRATPGSCTDAGAKHHPTPLHLH 60

Query: 61  SHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFS 120
           SH  GGD ILPKRSTKKR E  ASVGGGGSVSCSKCRPHAREKFSVVP+D NGVAKQFFS
Sbjct: 61  SH--GGDEILPKRSTKKRLEPVASVGGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFS 120

Query: 121 MASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAILE 180
           MASPNGILKSIVSS T KSPKSM E  DW  REE WKIA GE+SHKLI+ATRKRDEAILE
Sbjct: 121 MASPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFGEVSHKLIEATRKRDEAILE 180

Query: 181 ASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFLS 240
           ASRLKYSMAELEKKLNKLEIYCH+LKS L +C SNPR P  EVPTNSD ISD+IIENFLS
Sbjct: 181 ASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLS 240

Query: 241 SVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHLEAIL 300
           SVSESRSSVRQLSRSLAMQL H+GGKI+ERIQ LL+SHEIK   SK+ +TSL+FHLEAIL
Sbjct: 241 SVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAIL 300

Query: 301 NRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFSR 360
           NRAFFEDFESIGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVL KGTRHFSE+FSR
Sbjct: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSR 360

Query: 361 FCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFD 420
           FCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RFD
Sbjct: 361 FCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFD 420

Query: 421 GVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           GVYMEDIAGEK RELAPATVRIMV+PGFY FD LIKCKV+CRYH+NLNN
Sbjct: 421 GVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYHTNLNN 466

BLAST of Spg016034 vs. ExPASy TrEMBL
Match: A0A6J1HMP3 (IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111465023 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.1e-225
Identity = 406/470 (86.38%), Postives = 426/470 (90.64%), Query Frame = 0

Query: 1   MKNITPSSSPS-PLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHH 60
           MKNITPSSSPS PLHH QSSPFFSI  NSERTEEEDEFNRATPSS TD GAKH+PTPLH 
Sbjct: 1   MKNITPSSSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPSSCTDDGAKHHPTPLHL 60

Query: 61  HSHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120
           HSH  GGD ILPKRSTKKR E  ASVG GGSVSCSKCRPHAREKFSVVP+D NGVAKQFF
Sbjct: 61  HSH--GGDEILPKRSTKKRLEPVASVGDGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFF 120

Query: 121 SMASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAIL 180
           SMASPNGILKSIVSSFT KSPKSM E  DW  REEHWKIA GE+SHKLI+ATRKRDEAIL
Sbjct: 121 SMASPNGILKSIVSSFTGKSPKSMKEGADWAVREEHWKIAFGEVSHKLIEATRKRDEAIL 180

Query: 181 EASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFL 240
           EASRLKYSMAELEKKLNKLEIYCH+LKS L +C SNPR P  EVPTNSD ISD+IIENFL
Sbjct: 181 EASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFL 240

Query: 241 SSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHLEAI 300
           SSVSESRSS+RQLSRSLAMQL H+G KI+ERIQ LL+SH+IK   SK+ +TSL+FHLEAI
Sbjct: 241 SSVSESRSSMRQLSRSLAMQLHHIGDKIYERIQFLLRSHDIKISLSKNSKTSLIFHLEAI 300

Query: 301 LNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFS 360
           LNRAFFEDFESIGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVL KGTRHFSE+FS
Sbjct: 301 LNRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFS 360

Query: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARF 420
           RFCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLATAVHPSLPIFRVDSG RF
Sbjct: 361 RFCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLATAVHPSLPIFRVDSGVRF 420

Query: 421 DGVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           DGVYMEDIAG+K RELAPATVRIMV+PGFY FD LIKCKV CRYH+NLNN
Sbjct: 421 DGVYMEDIAGDKARELAPATVRIMVAPGFYAFDGLIKCKVTCRYHTNLNN 468

BLAST of Spg016034 vs. ExPASy TrEMBL
Match: A0A1S3BLQ8 (IRK-interacting protein-like OS=Cucumis melo OX=3656 GN=LOC103490937 PE=4 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.5e-219
Identity = 404/473 (85.41%), Postives = 423/473 (89.43%), Query Frame = 0

Query: 1   MKNITPSSSPSPLHHCQSSPFFSITQNSERTEEE----DEFNRATPSSRTDAGAKHNPTP 60
           MKNITP           SSPFFSI   ++RTEEE    DEFN+ATP S TDAGAKH+PTP
Sbjct: 1   MKNITP-----------SSPFFSIIHKTDRTEEEEEEDDEFNKATPCSGTDAGAKHHPTP 60

Query: 61  LHHHSHGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAK 120
           LH H    G DG+LPKRSTKK P+SAASV   GSVSCSKCRPHAREKFSVVPLDNNGVAK
Sbjct: 61  LHLH----GSDGVLPKRSTKKHPDSAASV-STGSVSCSKCRPHAREKFSVVPLDNNGVAK 120

Query: 121 QFFSMASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDE 180
           QFFSMASPNG+LKSIVSS TRKSPK +NE TD  AREEHWKIALGEISHKLIQATRKRDE
Sbjct: 121 QFFSMASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDE 180

Query: 181 AILEASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIE 240
           AILEASRLKYSMAELEKKLNKLEIYCHTLKS LDECASNPRKPK EV  NSD ISD+IIE
Sbjct: 181 AILEASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKPKSEVQKNSDSISDKIIE 240

Query: 241 NFLSSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK---SKSPRTSLMFHL 300
           NFLSSVSESRSSVRQLSRSLAMQLR +GGK++ERIQ LLQSH+IK   SK+ +TSL+FHL
Sbjct: 241 NFLSSVSESRSSVRQLSRSLAMQLRQIGGKVYERIQFLLQSHDIKIPLSKNLKTSLIFHL 300

Query: 301 EAILNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSE 360
           EAILNRAFFEDFE+IGFQKNSPN ILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSE
Sbjct: 301 EAILNRAFFEDFETIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSE 360

Query: 361 EFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG 420
           +FSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG
Sbjct: 361 DFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG 420

Query: 421 ARFDGVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
            RFDGVYMEDIAGEK RELAPATVRIMVSPGFYVFDNLIKCKV+CRYH NLNN
Sbjct: 421 VRFDGVYMEDIAGEKARELAPATVRIMVSPGFYVFDNLIKCKVVCRYHINLNN 457

BLAST of Spg016034 vs. ExPASy TrEMBL
Match: A0A6J1FU57 (IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448164 PE=4 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 2.1e-218
Identity = 395/467 (84.58%), Postives = 423/467 (90.58%), Query Frame = 0

Query: 1   MKNITPSSSPSPLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHHH 60
           MKNI P  SPSPLHHC+SS   SIT NSERTEEEDEFNRATP SR       + TPLHH+
Sbjct: 1   MKNIAP--SPSPLHHCRSSALSSITHNSERTEEEDEFNRATPRSR-----PASQTPLHHY 60

Query: 61  SHGG-GGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120
           S GG GGDGILPKRSTKKR E AASV GGGSV C+KCRPH REKFSVVPLD+NGVAKQ F
Sbjct: 61  SLGGCGGDGILPKRSTKKRQELAASVNGGGSVLCTKCRPHVREKFSVVPLDSNGVAKQIF 120

Query: 121 SMASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAIL 180
           SMASP+G+LKSIV+S TRKSPKSM EETDW AREE WKIALGEISH+LI ATR+RDEAIL
Sbjct: 121 SMASPSGMLKSIVTSLTRKSPKSMKEETDWAAREEQWKIALGEISHRLIHATRRRDEAIL 180

Query: 181 EASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFL 240
           EASRLK SMAELEKKLNKLEIYCHTL+SGLDECASNPR PKQE  TNSDPI D++I NFL
Sbjct: 181 EASRLKNSMAELEKKLNKLEIYCHTLRSGLDECASNPRTPKQEFSTNSDPIPDKLITNFL 240

Query: 241 SSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIKSKSPRTSLMFHLEAILNR 300
           SSVSESRSS+RQLSRSLAMQLRH+GGK +ERIQILL     KSKSPRTSL+FHLEAILNR
Sbjct: 241 SSVSESRSSMRQLSRSLAMQLRHVGGKTYERIQILL-----KSKSPRTSLLFHLEAILNR 300

Query: 301 AFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFSRFC 360
           AFFEDFESIGFQKNSPN ILNPS+RTESNIASFN+LHRLSWEEVLSKGTRHFSEEFSRFC
Sbjct: 301 AFFEDFESIGFQKNSPNQILNPSNRTESNIASFNQLHRLSWEEVLSKGTRHFSEEFSRFC 360

Query: 361 DRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGV 420
           DRKMSDIVA+LEWNRAWPEPLLQAFFAAAKSVWLVHL+ATAVHPSLPIFRV++G +FD V
Sbjct: 361 DRKMSDIVAILEWNRAWPEPLLQAFFAAAKSVWLVHLIATAVHPSLPIFRVETGVKFDSV 420

Query: 421 YMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           YMEDIAG+KTRE+APATVRIMVSPGFYVFDNLIKCKVLCRYH+NL+N
Sbjct: 421 YMEDIAGDKTREVAPATVRIMVSPGFYVFDNLIKCKVLCRYHANLDN 455

BLAST of Spg016034 vs. ExPASy TrEMBL
Match: A0A6J1KZ33 (IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111498403 PE=4 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 1.1e-217
Identity = 394/467 (84.37%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 1   MKNITPSSSPSPLHHCQSSPFFSITQNSERTEEEDEFNRATPSSRTDAGAKHNPTPLHHH 60
           MKNI P  SPSPLHHC+SS   SIT NSERTEEEDEFNRATP SR       + TPLHH+
Sbjct: 1   MKNIAP--SPSPLHHCRSSVLSSITHNSERTEEEDEFNRATPRSR-----PASLTPLHHY 60

Query: 61  S-HGGGGDGILPKRSTKKRPESAASVGGGGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120
           S  G G DGILPKRSTKKR E AASV GGGSVSC+KCRPH REKFSVVPLD+NGVAKQ F
Sbjct: 61  SLDGCGSDGILPKRSTKKRQELAASVNGGGSVSCTKCRPHVREKFSVVPLDSNGVAKQIF 120

Query: 121 SMASPNGILKSIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAIL 180
           SMASP+G+LKSIV+S TRKSPKSM EETDW AREE WKIALGEISH+LI ATR+RDEAIL
Sbjct: 121 SMASPSGMLKSIVTSLTRKSPKSMKEETDWAAREEQWKIALGEISHRLIHATRRRDEAIL 180

Query: 181 EASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSDPISDRIIENFL 240
           EASRLK SMAELEKKLNKLEIYCHTLKSGLDECASNPR PKQE+ TNSDPI D++I NFL
Sbjct: 181 EASRLKNSMAELEKKLNKLEIYCHTLKSGLDECASNPRTPKQEISTNSDPIPDKLIPNFL 240

Query: 241 SSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIKSKSPRTSLMFHLEAILNR 300
           SSVSESRSS+RQLSRSLAMQLRH+GGK +ERIQILL     KSKSPRTSL+FHLEAILNR
Sbjct: 241 SSVSESRSSMRQLSRSLAMQLRHVGGKTYERIQILL-----KSKSPRTSLLFHLEAILNR 300

Query: 301 AFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFSRFC 360
           AFFEDFESIGFQKNSPN ILNPS+RTESNIASFNRLHRLSWEEVLSKGTRHFSEEFSRFC
Sbjct: 301 AFFEDFESIGFQKNSPNQILNPSNRTESNIASFNRLHRLSWEEVLSKGTRHFSEEFSRFC 360

Query: 361 DRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGV 420
           DRKMSDIVA+LEWNRAWPEPLLQAFFAAAKSVWLVHL++TA HPSLPIFRV++G +FD V
Sbjct: 361 DRKMSDIVAILEWNRAWPEPLLQAFFAAAKSVWLVHLISTAAHPSLPIFRVETGVKFDSV 420

Query: 421 YMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLCRYHSNLNN 467
           YMEDIAG+KTRE+APATVRIMVSPGFYVFDNLIKCKVLCRYH+NL+N
Sbjct: 421 YMEDIAGDKTREVAPATVRIMVSPGFYVFDNLIKCKVLCRYHANLDN 455

BLAST of Spg016034 vs. TAIR 10
Match: AT1G12330.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12900.1); Has 249 Blast hits to 249 proteins in 27 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 14; Plants - 217; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 463.4 bits (1191), Expect = 2.2e-130
Identity = 266/481 (55.30%), Postives = 338/481 (70.27%), Query Frame = 0

Query: 5   TPSSSPSPLHHCQSSPFFSITQNSERTEEEDEF-NRATPSSRTDAGAKHNPTPLHH---H 64
           +PS SP P  H QS  F  I +  E   +E+ + NRATPSS  D G+   P   HH   +
Sbjct: 8   SPSKSPVPSLH-QSLHFTPILECEEDDLQEERYKNRATPSS--DGGSSATPNHQHHRRTN 67

Query: 65  SHGGGGDGILP-----KRSTKKRPESAASVGGGGSVSCSKCRPH--AREKFSVVPLDNNG 124
           S+      + P     K  T+KR +       GG+VSC+KCRPH   R+KFSVVPL+++ 
Sbjct: 68  SNNNHQHTLTPLHHNGKPQTRKRHDD-NDEDDGGAVSCNKCRPHHSHRDKFSVVPLESHN 127

Query: 125 VAKQFFSMASPNGILKSIVSSFTRKSPK-----------SMNEETDWTAREEHWKIALGE 184
                  ++SPN I+KSI  S TR+SPK           S +      +REE W++A+ E
Sbjct: 128 NPS---FISSPNLIIKSIFQSLTRRSPKPSSATAALPPRSSSSSAADASREEQWRLAVAE 187

Query: 185 ISHKLIQATRKRDEAILEASRLKYSMAELEKKLNKLEIYCHTLKSGLDECASNPRKPKQE 244
           +SHKLIQAT+K+++A++EASRLK SMAELEKKLNKLEIYCH LKSGLDEC++     KQ 
Sbjct: 188 LSHKLIQATKKKEDAVIEASRLKSSMAELEKKLNKLEIYCHNLKSGLDECSNK----KQS 247

Query: 245 VPTNSDPISDRIIENFLSSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIK- 304
           VP   D  +DRII+ FL SVSESRSS+R LSRSLA QLR +GGK++ER+ +LLQ  ++K 
Sbjct: 248 VPIRKDGFNDRIIQQFLVSVSESRSSIRALSRSLASQLRTVGGKVYERLSLLLQPFDVKI 307

Query: 305 ---SKSPRTSLMFHLEAILNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRL 364
              +K+P+ SL+F+LEAIL+RAFFEDFE+ GFQKN    ILNP DR ESN ASFN L  L
Sbjct: 308 NSFAKNPK-SLIFYLEAILSRAFFEDFEAPGFQKNGSTRILNPIDRCESNYASFNVLMEL 367

Query: 365 SWEEVLSKGTRHFSEEFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLA 424
           +W+EVLS+GT+HFSEEFSRFCDRKMSD+V+ML WNRAWPEPLLQAFF A+KSVWLVHLLA
Sbjct: 368 TWDEVLSRGTKHFSEEFSRFCDRKMSDVVSMLSWNRAWPEPLLQAFFGASKSVWLVHLLA 427

Query: 425 TAVHPSLPIFRVDSGARFDGVYMEDIAGEKTRELAPATVRIMVSPGFYVFDNLIKCKVLC 460
            +V+P L IFRV+   RFD +YME+  GE+ + L    VR MV PGFYV+ +++KCKV+C
Sbjct: 428 NSVNPGLQIFRVEKDDRFDPIYMEETGGERFKSL----VRAMVQPGFYVYGSVVKCKVVC 472

BLAST of Spg016034 vs. TAIR 10
Match: AT5G12900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12330.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 246.9 bits (629), Expect = 3.2e-65
Identity = 128/311 (41.16%), Postives = 189/311 (60.77%), Query Frame = 0

Query: 162 EISHKLIQATRKRDEAILEASRLKYSMAELEKKLNKLEIYCHTLKSGLDECA-----SNP 221
           ++  +L++A R RD A+ + S +K S+ EL +KL  LE YC  LK  L E        N 
Sbjct: 250 KLKRELMEANRSRDAALTQVSEMKSSLGELSEKLQYLESYCDNLKKALREATEVVSQENS 309

Query: 222 RKPKQEVPTNSDPISDRI-IENFLSSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILL 281
                    +  P+S+ + +E FL  VSE+R S++Q  ++L  ++      +   I  LL
Sbjct: 310 GGRSSGKKNSEMPVSEEVMVEGFLQIVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLL 369

Query: 282 QSHEIKSKSPRTSLM-FHLEAILNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNR 341
           Q H +   S  + ++ +HLEAI++++ ++DFE+  FQKN    +L+P    ++N +SF  
Sbjct: 370 QPHNLSFTSKYSKIIQYHLEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFAS 429

Query: 342 LHRLSWEEVLSKGTRHFSEEFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLV 401
           L  LSW EVL KGT+++S+EFSRFCD KMS I+  L W R W E +LQAFF AAK VWL+
Sbjct: 430 LRNLSWNEVLKKGTKYYSDEFSRFCDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVWLL 489

Query: 402 HLLATAVHPSLPIFRVDSGARFDGVYMEDIAGEKTREL---APATVRIMVSPGFYVFDNL 461
           HLLA + +P+L I RV+    F+  +MED+  ++ R      PA V++MV PGFYV D +
Sbjct: 490 HLLAFSFNPALGILRVEENREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFYVLDRV 549

Query: 462 IKCKVLCRYHS 463
           ++CKVLCRY S
Sbjct: 550 LRCKVLCRYKS 560

BLAST of Spg016034 vs. TAIR 10
Match: AT3G60680.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-10
Identity = 80/358 (22.35%), Postives = 147/358 (41.06%), Query Frame = 0

Query: 130 SIVSSFTRKSPKSMNEETDWTAREEHWKIALGEISHKLIQATRKRDEAILEASRLKYSMA 189
           S +  F R   +S++ E+D         +A+G      +Q  + +  A+   S       
Sbjct: 166 SDLKQFYRNYRQSLDFESD---------LAIGSCLESRVQENQSKLRALETVSN------ 225

Query: 190 ELEKKLNKLEIYCHTLKSGLDECASNPRKPKQEVPTNSD---PISDRIIENFL----SSV 249
            L+ +++  ++   +L++ L E   +  K  + + +NS     +S R+ E+ L     + 
Sbjct: 226 RLQAEMDAKDLQVWSLRNKLGEIQKSTSKLSKRLSSNSSLDVLLSVRVFESLLYDAFKAT 285

Query: 250 SESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIKSKSPRTSLMFHLEAILNRAFF 309
            +    + +L       L  +   +H  +    + H            + L + +    F
Sbjct: 286 QKFTKILIELMEKAGWDLDLVAKSVHPEVDYAKERHN----------RYALLSYVCLGMF 345

Query: 310 EDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEE----FSRF 369
             F+  GF        LN +D  ES  +S +   R   + V S        +    FSRF
Sbjct: 346 RGFDGEGFD-------LNENDYEESERSSVDSSLRELMQHVSSNPMELLDRDKDCAFSRF 405

Query: 370 CDRK---------MSDIVAMLEWNRA----WP--EPLLQAFFAAAKSVWLVHLLATAVHP 429
           CD+K          S I + ++ N A    W       ++F   A S+W +H LA +  P
Sbjct: 406 CDKKYHELIHPNMASSIFSNMDENEAVLSSWRSLSTFYESFVTMASSIWTLHKLALSFDP 465

Query: 430 SLPIFRVDSGARFDGVYMEDI---AGEKTRELAP--ATVRIMVSPGFYVFDNLIKCKV 457
           ++ IF+V+SG  F  V+ME++     +K   ++P  A V   V PGF +   +I+C+V
Sbjct: 466 AVEIFQVESGVEFSIVFMENVLKRKQDKKFSMSPTRAKVGFTVVPGFKIGCTVIQCQV 491

BLAST of Spg016034 vs. TAIR 10
Match: AT2G45260.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-08
Identity = 59/243 (24.28%), Postives = 102/243 (41.98%), Query Frame = 0

Query: 232 DRIIENFLSSVSESRSSVRQLSRSLAMQLRHMGGKIHERIQILLQSHEIKSKSPRTSLMF 291
           D   E ++S+   +  +V   S+ L   ++  G  +       ++   + +K P     F
Sbjct: 181 DLTTELYVSTYEAAAKAVHDFSKPLINMMKAAGWDLDSAAN-SIEPDVVYAKRPHKKYAF 240

Query: 292 HLEAILNRAFFEDFESIGFQKNSPNPILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHF 351
             E+ + +  F  F+   F  NS +  +  +D T++    F  L  +   + L  GT   
Sbjct: 241 --ESYICQRMFSGFQQKNFSVNSESAAVMANDDTDTFFRQFLALKDMDPLDAL--GTNPD 300

Query: 352 SEEFSRFCDRKMSDIV------------AMLEWNRAWPEP---LLQAFFAAAKSVWLVHL 411
           S  F  FC  K   +V               ++      P     QAF   AKS+W++H 
Sbjct: 301 S-NFGIFCRSKYLLLVHPKMEASFFGNLDQRDYVTGGGHPRTAFYQAFLKLAKSIWILHR 360

Query: 412 LATAVHPSLPIFRVDSGARFDGVYMEDIAGE---KTRELAPATVRIMVSPGFYVFDNLIK 457
           LA +  P+  IF+V  G+ F   YME +        +E  P  V +MV PGF++  ++I+
Sbjct: 361 LAYSFDPAAKIFQVKKGSEFSDSYMESVVKNIVVDEKEENP-RVGLMVMPGFWIGGSVIQ 416

BLAST of Spg016034 vs. TAIR 10
Match: AT5G58960.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 58.2 bits (139), Expect = 2.1e-08
Identity = 53/184 (28.80%), Postives = 74/184 (40.22%), Query Frame = 0

Query: 280 IKSKSPRTSLMFHLEAILNRAFFEDFESIGF-QKNSPNPILNPSDRTESNIASFNRLHRL 339
           ++S  P     F LE+ + R  F+ F+   F    S + ++NP        A F  +  +
Sbjct: 359 VQSSVPNQHAKFALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAM 418

Query: 340 SWEEVLS-KGTRHFSEEFSRFCDRKMSDIV------------AMLEWNRAWPEPLLQ--- 399
              E+L    T H    F +FC +K   I+               E   A   P  Q   
Sbjct: 419 DPMELLGILPTCH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYG 478

Query: 400 AFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIAG-EKTRELAPATVRIMV 446
            F   AK+VWL+HLLA ++ PS   F  + GA F   YME +      R  A   V   V
Sbjct: 479 EFLGLAKAVWLLHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPV 538