Spg015855 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg015855
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold6: 42875416 .. 42875913 (+)
RNA-Seq ExpressionSpg015855
SyntenySpg015855
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTCGGATGCTTCGTGGAACGATGATTGGAGAATTGGTGGTGTTGGTTGGATCGTTCGTGATTCTTCCGAATCTCTAATTCAGGCGGGTTACAAAAGAGTCAATCGCAAATGACAAATTAAATCCCTAGAAGCTTTAGCTATTCGAGAAGGGCTAAAAGCAATTAAATTGAAGGATCAGCCAAGGAACTTGCCGTTAATTGCTGAAGCAAATGCCCTCGAGGTTGTGAAAGTGCTGAATCATGAAATCCAAGACATCTCGGACACCAAAGTGATAGTCAACGAGGTGGAAGAGCTTGCGTTTGAGGTAGGAGTCATCTCTTTCTCAAAATGCCCTAGGGTGGGCAATCGAATTGCGCATTCACTCGCGCGAGCTGCGGCAGGGTTCATGCCGGAGCATCCTCTGTCGTCCGTCGAAGTCGACAGCAACGTCCATGGTTTTGGGTCAACTAGTTTTGGTCTTCCTTCCACGCTGGAAGAAGAATTTTTTTTGTAG

mRNA sequence

ATGAACTCGGATGCTTCGTGGAACGATGATTGGAGAATTGGTGGTGTTGGTTGGATCGTTCAAGCTTTAGCTATTCGAGAAGGGCTAAAAGCAATTAAATTGAAGGATCAGCCAAGGAACTTGCCGTTAATTGCTGAAGCAAATGCCCTCGAGGTTGTGAAAGTGCTGAATCATGAAATCCAAGACATCTCGGACACCAAAGTGATAGTCAACGAGGTGGAAGAGCTTGCGTTTGAGGTAGGAGTCATCTCTTTCTCAAAATGCCCTAGGGTGGGCAATCGAATTGCGCATTCACTCGCGCGAGCTGCGGCAGGGTTCATGCCGGAGCATCCTCTGTCGTCCGTCGAAGTCGACAGCAACGTCCATGGTTTTGGGTCAACTAGTTTTGGTCTTCCTTCCACGCTGGAAGAAGAATTTTTTTTGTAG

Coding sequence (CDS)

ATGAACTCGGATGCTTCGTGGAACGATGATTGGAGAATTGGTGGTGTTGGTTGGATCGTTCAAGCTTTAGCTATTCGAGAAGGGCTAAAAGCAATTAAATTGAAGGATCAGCCAAGGAACTTGCCGTTAATTGCTGAAGCAAATGCCCTCGAGGTTGTGAAAGTGCTGAATCATGAAATCCAAGACATCTCGGACACCAAAGTGATAGTCAACGAGGTGGAAGAGCTTGCGTTTGAGGTAGGAGTCATCTCTTTCTCAAAATGCCCTAGGGTGGGCAATCGAATTGCGCATTCACTCGCGCGAGCTGCGGCAGGGTTCATGCCGGAGCATCCTCTGTCGTCCGTCGAAGTCGACAGCAACGTCCATGGTTTTGGGTCAACTAGTTTTGGTCTTCCTTCCACGCTGGAAGAAGAATTTTTTTTGTAG

Protein sequence

MNSDASWNDDWRIGGVGWIVQALAIREGLKAIKLKDQPRNLPLIAEANALEVVKVLNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIAHSLARAAAGFMPEHPLSSVEVDSNVHGFGSTSFGLPSTLEEEFFL
Homology
BLAST of Spg015855 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 60.1 bits (144), Expect = 1.8e-05
Identity = 35/132 (26.52%), Postives = 66/132 (50.00%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWI------------------------VQALAIREGLKAIKLK- 60
           +N+DA+W  D   GG+GWI                        ++ +AI EGL+AI+ + 
Sbjct: 89  LNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEH 148

Query: 61  ----DQPRNLPLIAEANALEVVKVLNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRV 104
                Q    P+  E+++LE + +L+ + QD ++   ++ E+ ++  ++ ++S     R 
Sbjct: 149 CRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISRE 208

BLAST of Spg015855 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 59.7 bits (143), Expect = 2.4e-05
Identity = 40/128 (31.25%), Postives = 63/128 (49.22%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWIV------------------------QALAIREGLKAIKLKD 60
           +N+DASW+D    GG+GWI+                        +A AI EGL+ +    
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 61  QPRNLPLIAEANALEVVKVLNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIA 105
             R  PL  E ++ EV  +LN + +D++ T  +V E+  L     +++F+K  R  N  A
Sbjct: 141 VLR--PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCA 200

BLAST of Spg015855 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 59.3 bits (142), Expect = 3.1e-05
Identity = 34/127 (26.77%), Postives = 65/127 (51.18%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWI------------------------VQALAIREGLKAIKLKD 60
           +N++A+W  D   GG+GWI                        ++ +AI EGL+AI+   
Sbjct: 89  LNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIR--- 148

Query: 61  QPRNLPLIAEANALEVVKVLNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIA 104
           Q    P+  E+++LE + +L+ + QD ++   ++ E+ ++  ++ ++S     R  N++A
Sbjct: 149 QEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVA 208

BLAST of Spg015855 vs. NCBI nr
Match: XP_022148549.1 (uncharacterized protein LOC111017181 [Momordica charantia])

HSP 1 Score: 57.8 bits (138), Expect = 8.9e-05
Identity = 37/122 (30.33%), Postives = 61/122 (50.00%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWIVQ----ALAIREGLKAIKLK-DQPRNLPLIAEANALEVVKV 60
           +N DA+W D    GG+GWIV+       + E LKA+         + +  E++ LEVV +
Sbjct: 88  LNVDATWMDSLHAGGLGWIVRDSEGRFIMAECLKALSQSLTLEAGIKIEMESDCLEVVNI 147

Query: 61  LNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIAHSLARAAAGFMPEHPLSSV 118
           +N     +++  +IV ++ +    + +  F   P   N +AH +AR A  F+ EH L  V
Sbjct: 148 INKSSMVLTEVSLIVEDIWKEMESLPIEGFKHLPMKANGVAHGIARRACVFV-EHVLPYV 207

BLAST of Spg015855 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 8.7e-06
Identity = 35/132 (26.52%), Postives = 66/132 (50.00%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWI------------------------VQALAIREGLKAIKLK- 60
           +N+DA+W  D   GG+GWI                        ++ +AI EGL+AI+ + 
Sbjct: 89  LNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQEH 148

Query: 61  ----DQPRNLPLIAEANALEVVKVLNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRV 104
                Q    P+  E+++LE + +L+ + QD ++   ++ E+ ++  ++ ++S     R 
Sbjct: 149 CRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISRE 208

BLAST of Spg015855 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-05
Identity = 40/128 (31.25%), Postives = 63/128 (49.22%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWIV------------------------QALAIREGLKAIKLKD 60
           +N+DASW+D    GG+GWI+                        +A AI EGL+ +    
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 61  QPRNLPLIAEANALEVVKVLNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIA 105
             R  PL  E ++ EV  +LN + +D++ T  +V E+  L     +++F+K  R  N  A
Sbjct: 141 VLR--PLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAKVGRETNGCA 200

BLAST of Spg015855 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.5e-05
Identity = 34/127 (26.77%), Postives = 65/127 (51.18%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWI------------------------VQALAIREGLKAIKLKD 60
           +N++A+W  D   GG+GWI                        ++ +AI EGL+AI+   
Sbjct: 89  LNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIR--- 148

Query: 61  QPRNLPLIAEANALEVVKVLNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIA 104
           Q    P+  E+++LE + +L+ + QD ++   ++ E+ ++  ++ ++S     R  N++A
Sbjct: 149 QEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVA 208

BLAST of Spg015855 vs. ExPASy TrEMBL
Match: A0A6J1D4B6 (uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017181 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.3e-05
Identity = 37/122 (30.33%), Postives = 61/122 (50.00%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVGWIVQ----ALAIREGLKAIKLK-DQPRNLPLIAEANALEVVKV 60
           +N DA+W D    GG+GWIV+       + E LKA+         + +  E++ LEVV +
Sbjct: 88  LNVDATWMDSLHAGGLGWIVRDSEGRFIMAECLKALSQSLTLEAGIKIEMESDCLEVVNI 147

Query: 61  LNHEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIAHSLARAAAGFMPEHPLSSV 118
           +N     +++  +IV ++ +    + +  F   P   N +AH +AR A  F+ EH L  V
Sbjct: 148 INKSSMVLTEVSLIVEDIWKEMESLPIEGFKHLPMKANGVAHGIARRACVFV-EHVLPYV 207

BLAST of Spg015855 vs. ExPASy TrEMBL
Match: A0A5C7IQ65 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000112 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 6.3e-04
Identity = 33/104 (31.73%), Postives = 62/104 (59.62%), Query Frame = 0

Query: 1   MNSDASWNDDWRIGGVG---WIVQALAIREGLKAIKLKDQPRNLPLIAEANALEVVKVLN 60
           +N+DA+ N   ++ G G    I +ALAI EGL+    ++  +   +  E++AL VV+ +N
Sbjct: 134 INTDAALNFQLKVAGFGNKPPIAEALAILEGLRFAFSRNFSK---VCLESDALCVVQAIN 193

Query: 61  HEIQDISDTKVIVNEVEELAFEVGVISFSKCPRVGNRIAHSLAR 102
           ++    S+  V++N++  +      +SF+  PR+GN++AH LA+
Sbjct: 194 NKSPPSSEVGVVLNDIFLVLDRFSEVSFAFVPRLGNKVAHGLAK 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156777.11.8e-0526.52uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_022155262.12.4e-0531.25uncharacterized protein LOC111022403 [Momordica charantia][more]
XP_022143535.13.1e-0526.77uncharacterized protein LOC111013412 [Momordica charantia][more]
XP_022148549.18.9e-0530.33uncharacterized protein LOC111017181 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DSV18.7e-0626.52uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1DNV91.1e-0531.25uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1CP261.5e-0526.77uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1D4B64.3e-0530.33uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A5C7IQ656.3e-0431.73RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000112 ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 21..103
e-value: 8.6E-5
score: 22.3
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 2..101
e-value: 1.39162E-12
score: 58.4796

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg015855.1Spg015855.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity